LOCUS       NZ_BMSI01000012        12865 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence12, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000012
VERSION     NZ_BMSI01000012.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 316734)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 263822
            Orig. end                         :: 276687
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12865
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="2"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12865
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12865
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7865](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7865)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(553..1839)
                     /locus_tag="IE189_RS27350"
                     /old_locus_tag="GCM10010496_55690"
     CDS             complement(553..1839)
                     /EC_number="1.14.13.-"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FMO-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358093.1"
                     /locus_tag="IE189_RS27350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55690"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_189926032.1"
                     /sec_met_domain="FMO-like (E-value: 2e-73, bitscore: 241.6,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.1e-12, bitscore:
                     41.8, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLCVIGAGLSGLAVAHALKSAGVDFVCLEQASDVGGLWRRPQAG
                     ERGPGYLSLHLNTAKALTGYSDYPMPASYPLYPRHSQIAAYLRSFAEWAGVLDSVELGT
                     TVESVRQEADGGWTVVGRDAEGTVSSRAFSHVVVASGHNSEPSMPALPRGAETFTGTIH
                     HSVDYRDGSDYAGQRVVVVGLGNSAVDIASDLSRHAGQTVLSVRRGLHVMPKQLFGMAL
                     DEIADAPWWISMSLEEQRRFIEQTLLVARGKLRDHGLPEPDHPVFSSAVTISDEILSRI
                     RHGAVTPKPAIEAVDGDRVSFTDGTSVRADALVYCTGYRMTFPFLEPGCPVGAGGSVEL
                     YKRVVAPDRPGLFFAGLIRPVGSITRLVEAQARWIGRLATGAAVLPEAEVMHKEIAAYL
                     SGVAGQYGRREGASTQVNVAPYLQELREE"
     gene            complement(2041..2742)
                     /locus_tag="IE189_RS27355"
                     /old_locus_tag="GCM10010496_55700"
     CDS             complement(2041..2742)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385591.1"
                     /locus_tag="IE189_RS27355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55700"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_189925927.1"
                     /transl_table=11
                     /translation="MAALPESSSQQPAPLSLPVLGGRPSSYRERVADALRAALIAGELR
                     PGEVYSAPALATRFGVSATPVREAMLDLAKEGLVHAVPNKGFRVTEVSDRQLDEYTHVR
                     ALIEIPTVVALATTADPVSLEALRPAAREIVTAAVAGDLIAYVEADTRFHLGLLALAGN
                     THLVEVVRGLRGRARLYGLTALAASGRLLSSAEEHLELLDALLARDERAVHEVMTRHLG
                     HVRHLWASAPD"
     misc_feature    2841..2851
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            complement(2871..3872)
                     /locus_tag="IE189_RS27360"
                     /old_locus_tag="GCM10010496_55710"
     CDS             complement(2871..3872)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358096.1"
                     /locus_tag="IE189_RS27360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55710"
                     /product="proline racemase family protein"
                     /protein_id="WP_189925929.1"
                     /transl_table=11
                     /translation="MRSKLVLHAVDSHTEGMPTRVITGGIGTVPGATMNERRLYFREHR
                     DDVKRLLMNEPRGHAAMSGAILQPSTRADCDWGVVYIEVSGYLPMCGHGTIGVATVLVE
                     TGMVEVVEPVTTVRLDTPAGPVVAEVAVRDGAATSVTLRNVPSFVVALDRRATLPDGRT
                     VTYDLAYGGNFYAILPLSEFGLPFDRARKDDILRAGLSLMEVIAANDEPVHPEDASIRG
                     CHHVHLYAPGSTARRSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLHTEFVNES
                     FIGTHFTGRLLGTTEVAGRPAVLPSFTGRAWITGTAQYLLDPTDPFPAGFVL"
     gene            complement(3914..4897)
                     /locus_tag="IE189_RS27365"
                     /old_locus_tag="GCM10010496_55720"
     CDS             complement(3914..4897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042987.1"
                     /locus_tag="IE189_RS27365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55720"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_229901452.1"
                     /transl_table=11
                     /translation="MTASASASAPDFPAPGSPAAGSPAPAPAAAAAAAPRPWRGVLVAT
                     ALPLREDLSVDHDRYAEHCAWLVANGCDGVVPNGSLGEYQVLTPEERAAVVRTAVAAIG
                     GARVMPGVAAYGSAEARRWAEQAAEAGCGAVMLLPPNAYRADERAVVAHYAEVAGAGLP
                     VVAYNNPIDTKVDLVPELLARLHGEGHVQGVKEFSGDVRRAYRIAELAPELDLLVGADD
                     VLLELAVAGAKGWVAGYPNALPRASVELYRAAVAGDLTTALPLYRRLHPLLRWDSRVEF
                     VQAIKLSMDVVGRHGGRCRPPRVPLTPDQEATVRSATEKAVAAGLA"
     misc_feature    complement(4987..4997)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            complement(5001..6407)
                     /locus_tag="IE189_RS27370"
                     /old_locus_tag="GCM10010496_55730"
     CDS             complement(5001..6407)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358099.1"
                     /locus_tag="IE189_RS27370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55730"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_189925931.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 9.4e-27, bitscore:
                     88.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.9e-13, bitscore:
                     43.2, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEDRPVLAVIGAGPAGLAAALAAAAHGVQVVLVDSEPGPGGQFH
                     RQPAPGTAARRPPALRHRSRTWERLRAGLDAQLGIGRVTYLSDRHVWCVERASHGFTVH
                     ALAGPDQEESVAVRVDGVLLATGGYEKVLPFPGWTLPGVVTAGGAQALLKGGLAVPGRT
                     AVVAGTGPLLLPVATGLAAAGVDVVALVESAGAAGFVRRAGTWAAAPAKVTEAIAYATR
                     LLGRRVPVMTRHTVLAAHGDERLEAVSVAALDREGRPVPGTRRRVVCDTLAVGHGLLPH
                     TDLADGLGCRLDGPRVVVDEEQRTDVPGVWAAGETTGIGGAALSLAEGHIAGRSAAARL
                     TGAVPDPRAWAPAARARTRARAFFAALDAAYAARPVRWTEQVTDGTVVCRCEEVTAGAV
                     REAVDTLGAGDLRTVKLLTRAGMGWCQGRMCEPGVAGVAGCELRPARRLLARPVPLGVL
                     AAPDDPMDSS"
     gene            complement(6400..6699)
                     /locus_tag="IE189_RS27375"
                     /old_locus_tag="GCM10010496_55740"
     CDS             complement(6400..6699)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125680.1"
                     /locus_tag="IE189_RS27375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55740"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_189925933.1"
                     /sec_met_domain="Fer2_4 (E-value: 5e-29, bitscore: 94.4,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPREPLRDHPGDPFTLTFDGREIEALPGQTIAAALWTSGVTAWR
                     TTRGSGRARGVFCGIGVCFDCLVTVDGRPGRRACLVPAVPGAEVRTQHGTGHDD"
     gene            complement(6696..7865)
                     /locus_tag="IE189_RS27380"
                     /old_locus_tag="GCM10010496_55750"
     CDS             complement(6696..7865)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118956.1"
                     /locus_tag="IE189_RS27380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55750"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_189925935.1"
                     /sec_met_domain="DAO (E-value: 3.5e-62, bitscore: 205.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKRSCGDVVVVGAGMVGAACALYAARAGLDVILVDRGPVAGGTT
                     GAGEGNLLVSDKEPGPELRLALLSARLWGELAEETGPAVEYEAKGGLVVAATPEALGSL
                     TGLAAGQRAAGVTAVPVGADELHDLEPDLARGLAGGVLYPQDAQVMPTLAAARLVRASG
                     ARLETGRTVTRVLRDTTGAVRGVRTDRGDILAPAVVNAAGTWGAEVAALAGVGLPVLPR
                     RGFVLVTEPLPRRIRHKVYAADYVADVASDSAALQTSPVVEGTAAGPVLIGASRERVGF
                     DRSFSLPVVRALAAGATRLFPFLARVRAMRTYLGFRPYLPDHLPAVGPDPRVPGLFHAC
                     GHEGAGIGLATGTGHLIAQVLAGERPDLDLTPLRPDRFDDDRHDEEDTP"
     gene            8027..8731
                     /locus_tag="IE189_RS27385"
                     /old_locus_tag="GCM10010496_55760"
     CDS             8027..8731
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125683.1"
                     /locus_tag="IE189_RS27385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55760"
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="WP_189925937.1"
                     /transl_table=11
                     /translation="MTVTNILLSGIVGSTAYGLAREGSDVDRLGMFAAPTEQLHGLHQP
                     KESYVSTAPDRTLHEAAKWCRLALGGNPTVMELVWLPDELYEVRTPLGDELIALRETLL
                     SARRVRDAYLGYATQQFRKLQNRTGGTPSEDTRGRIAKNARHLKRLCTQGYELYSTGRL
                     TIRVDDPESYHRFGEQVADDPEAALPLLRGFEEAFAGTRSVLPEQPDETAADAWLRRVR
                     EEFYPAARTTPV"
     gene            9186..11273
                     /gene="fusA"
                     /locus_tag="IE189_RS27390"
                     /old_locus_tag="GCM10010496_55770"
     CDS             9186..11273
                     /GO_function="GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_process="GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="fusA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320074.1"
                     /locus_tag="IE189_RS27390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_55770"
                     /product="elongation factor G"
                     /protein_id="WP_189925939.1"
                     /transl_table=11
                     /translation="MRNNLDRQLSTVRNLGILAHVDAGKTTVTERILYATGTTHKRGEV
                     HDGTTVTDFDPQERDRGITIFAAAVSCEWAGHRINLIDTPGHVDFADEVERSLRVLDGA
                     VAVFDAVAGVEPQSESVWRQADRHGVPRIAFVNKLDRVGADLDTAVASIRDRLHPVPLV
                     VHLPIGAEDGFTGVVDLLRMRALTWSDGSDAAVEDDVPDDLLAEALRRRRLLEETVAER
                     HPAALEEFCDRETLSAGTLAAALRDLTRTGDGVVVLCGSAYRNRGVEPLLDAVVAYLPS
                     PLDVPPVRGVSGAAGGVPGGEERTADPSAPFAALAFKVTATATGRLTHLRVYSGTIGKG
                     DAVWDSGAGRTERIGRILRVQADRHAGLERAVAGDIVAVVGLKSARAGSTLSAPDAPVV
                     LEPPGVATPVVSVAVEARTGGDTDRLAVALARLAEEDPSLVVRTDPETGQTVLSGMGEL
                     HLEVAVEKIRRGLGLDVQVGRPRVTYRETVGRGVSGAVFRHVKQDGGAGQFAHVVLDVE
                     PLGADEAGDGAAAGFAFRSTVVGGRVPHEYVRAVEAGCRDALAEGPIGGHPVTGLRVTL
                     TDGATHVRDSSETAFRTAGRLGLREALRACAMVLLEPVVEVAVTVPEDAVGGVLGDLAA
                     RRGRVTDSAVRGGSAVVTATVPLAELFGYATRLRSRTQGRGTFSVRPTGYAPAPTPVTP
                     R"
     misc_feature    complement(11528..11547)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 26.14"
                     /tool="antismash"
     misc_feature    complement(12000..12020)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.44"
                     /tool="antismash"
     misc_feature    12143..12157
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.33"
                     /tool="antismash"
     misc_feature    12563..12576
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
ORIGIN
        1 cgccgccaac accggcccca cgctgtcggc ggcccgcaag gcgggcagca accgtgcgca
       61 cctcgcctgg agcgccggac cgcacgcctg cccctcgaag gagcccgccc ggcagatcgt
      121 cgtgacggcg atcgagaacc tcctcaaccg gctgcccgac gtcaacctga gcgttcccga
      181 ggagagcctg gcctggcgcc agggagcctt cagccgcggc ctcgtgacgc tcccggcgcg
      241 gttcacgccg accaagccgg tgagccggcc gggccgccag agcgcggtcc aggagccgcc
      301 gcgcgtgagc acgggagccc cgcgccaggc cgaacagtcc ggtgtgtgga gcaagttcct
      361 caactggctg accaagtgac actcgcgtcg cccacggcgt cgccgacgac cgcctgacgc
      421 cttcgccgca ccggcgaggc cacggcaccg gctgcgggga gccgggtccc ggtgccaccc
      481 gatacccgcc ctccggggcg ggtatctccg tcttcggagg ggtccgcggt ttcggcgcgg
      541 gccctcccgc cctcactcct cgcgcagctc ctggaggtag ggcgccacgt tgacctgcgt
      601 cgacgccccc tcgcgccggc cgtactggcc cgctacgccg ctcaggtacg cggcgatctc
      661 cttgtgcatg acctccgcct ccggcagtac ggcggcgccg gtggcgagcc gtccgatcca
      721 ccgtgcctgg gcctccacca gccgggtgat cgaaccgacg gggcggatca gcccggcgaa
      781 gaacagaccg ggacggtccg gggcgacgac ccgcttgtac agctcgaccg aacccccggc
      841 gcccaccggg cagcccggct ccaggaaggg gaacgtcatc cggtagccgg tgcagtacac
      901 gagggcgtcc gccctgaccg acgtgccgtc ggtgaaggag acccggtcgc cgtcgacggc
      961 ctcgatcgcc ggcttcggcg tgaccgcccc gtggcggatc cggctgagga tctcgtcgga
     1021 gatggtgacg gccgaggaga agaccggatg gtccggctcg ggcagcccgt gatcacggag
     1081 cttgccgcgg gcgacgagca gggtctgctc gatgaaacgg cgctgctcct ccagggacat
     1141 gctgatccac cagggcgcgt ccgcgatctc gtcgagggcc atgccgaaca gctgcttggg
     1201 catgacgtgc agtccgcggc gcaccgagag aacggtctgc ccggcgtgcc gggagaggtc
     1261 cgacgcgatg tccaccgcgg agttcccgag ccccacgacg acgacgcgct gtccggcgta
     1321 gtcgctgccg tcgcggtagt cgacggagtg gtgaatcgtt ccggtgaacg tctcggcgcc
     1381 tcgcgggagc gccggcatcg acggttccga gttgtgcccg gaggcgacga cgacatgcga
     1441 gaaggcccgg gacgagaccg taccctcggc gtcccggccg acgaccgtcc acccgccgtc
     1501 ggcctcctgc cgtaccgact ccaccgtcgt ccccagttcg acgctgtcga ggacaccggc
     1561 ccactcggcg aacgagcgca gataggcggc tatctgactg tgccgggggt acagcgggta
     1621 ggaggcgggc atcgggtagt ccgagtaacc cgtcagcgcc ttggcggtgt tgaggtggag
     1681 ggacagataa ccgggcccgc gctcgcccgc ctgcggccgg cgccacagcc cgccgacgtc
     1741 gctcgcctgc tccaggcaga cgaagtcgac accggcgctc ttcagggcgt gcgccacagc
     1801 cagccccgac agccccgcac cgatcacgca cagccgcacg gattccccca cagttcggcc
     1861 acccactcct catactccgg cacatggctg cccatcccgc gcgtacgtca ctcttggccc
     1921 gttcgcgaca agaatcacag cgtggtccgg gaacggacga ggtagggcag gcgggaggcg
     1981 ggaggcggga ggcgggaggc gggaggcggg gcggggcggg cggcccggcc gccgggcggg
     2041 ctaatccggt gcggaggccc acaggtgccg gacatggccg agatgcctgg tcatgacctc
     2101 gtggacggcc cgttcgtcgc gggccagcaa agcgtccagc agctccaggt gttcctcggc
     2161 ggaggagagc agccggccgg acgcggcgag cgcggtcagc ccgtacaggc gggcgcggcc
     2221 gcgcagcccg cggaccacct cgaccagatg cgtgttgccc gcgagcgcga gcagtcccag
     2281 gtggaagcgg gtgtccgcct cgacgtaggc gatgaggtcg cccgcgacgg ccgccgtgac
     2341 gatctcccgg gcggccggac gcagcgcctc cagggacacc gggtccgcgg tcgtcgccag
     2401 cgcgaccacg gtggggatct cgatgagcgc gcggacgtgc gtgtactcgt cgagctgccg
     2461 gtcggagacc tccgtgacac ggaacccctt gttgggcacg gcgtgcacca ggccctcctt
     2521 ggccaggtcg agcatcgcct cgcgcaccgg ggtcgccgag acgccgaagc gggtcgccag
     2581 ggcgggcgcc gagtacacct cgcccggccg cagttccccc gcgatcagcg ccgcgcgcag
     2641 cgcgtcggcg acccgctcgc gatagctgct gggccggccg ccgagcaccg gcagcgacaa
     2701 gggggcgggc tgctggctgc tgctctccgg cagggcggcc atgggggatc tcctcggatg
     2761 cgggctgtgc cttctgcggc ctctcgacgc ggaccgtgcc gtgtcgaaag gccgacgtgt
     2821 ggcggtgacg caggcacctg tgccatatca cgtgccacca ttgtccacgg tcacaggacg
     2881 aagcccgcgg ggaacgggtc ggtcggatcc agcaggtact gcgccgtacc cgtgatccag
     2941 gcgcggccgg tgaagctcgg cagcaccgcc gggcgccccg cgacctccgt ggtgccgagc
     3001 agccggcccg tgaagtgcgt cccgatgaac gactcgttga cgaactcggt gtgcaacggc
     3061 agctcgcccc gcgcgtgcag ctgcgccatg cgggcgctcg tccccgtgcc gcagggcgaa
     3121 cggtcgaacc agcccgggtg gatggccatc gcgtgcctgg accgccgggc ggtggagccc
     3181 ggcgcgtaca ggtgcacgtg gtgacagcca cggatggagg cgtcctcggg atggaccggc
     3241 tcgtcgttcg cggcgatcac ctccatcagc gacagcccgg cccgcaggat gtcgtccttg
     3301 cgtgcgcggt cgaagggcag cccgaactcc gacagcggca ggatcgcgta gaagttgccg
     3361 ccgtacgcga ggtcgtacgt caccgtgcgt ccgtcgggca gggtcgcccg gcggtccagg
     3421 gcgaccacga aggaggggac gttgcgcaag gtcacggacg tggccgcgcc gtcccggacg
     3481 gccacctcgg cgacgaccgg tccggccggg gtgtccaggc ggacggtggt gaccggttcg
     3541 accacctcga ccatgccggt ctccaccagt accgtcgcga ccccgatggt gccgtgcccg
     3601 cacatcggga ggtatcccga gacctcgatg tagacgacac cccagtcgca gtcggcccgt
     3661 gtcgacggct ggaggatcgc gccgctcatg gcggcgtgtc cgcgcggctc gttcatcagc
     3721 aaccgcttga cgtcgtcgcg gtgttcacgg aagtacagcc gccgctcgtt catcgtcgcg
     3781 ccgggcacgg tgccgatccc gccggtgatc acgcgggtgg gcatgccctc ggtgtgcgag
     3841 tcgacggcgt gcaggacgag cttgctgcgc acggagtgtc cctctctgtc gtgctcggtc
     3901 gtgggccggg cggtcaggcc aggcccgccg cgacggcctt ctcggtggcc gagcggacgg
     3961 tcgcctcctg gtccggcgtc agcggcaccc gtggcgggcg gcagcgaccg ccgtgacggc
     4021 ccacgacgtc catcgagagc ttgatcgcct ggacgaactc gacgcgcgag tcccagcgca
     4081 gcagcgggtg cagtcggcgg tagaggggaa gcgcggtggt gagatcaccg gccacggccg
     4141 cccggtagag ctcgaccgag gccctcggaa gcgcgttggg gtagccggcc acccagccct
     4201 tggcgccggc caccgccagt tccagcagga cgtcgtcggc cccgaccaac aggtcgagtt
     4261 ccggagcgag ttcggcgatc cggtaggcgc ggcgtacatc gcccgagaac tccttgaccc
     4321 cttggacgtg tccctcgccg tgcagccggg ccagcagttc gggcacgagg tcgaccttgg
     4381 tgtcgatcgg gttgttgtac gccaccaccg gcaggcccgc cccggcgacc tccgcgtagt
     4441 gggccacgac ggcccgttcg tcggcgcggt aggcgttcgg cgggagcagc atcaccgccc
     4501 cgcagcccgc ctcggccgcc tgctcggccc agcggcgggc ctccgccgag ccgtaggcgg
     4561 ccacgcccgg catcacccgc gccccgccga tcgcggcgac cgcggtccgc accaccgcgg
     4621 cccgttcctc gggcgtgagc acctggtact cgcccaggga tccgttcggt accacgccgt
     4681 cgcagccgtt cgccaccagc caggcgcagt gttcggcgta gcggtcgtgg tcgacggaga
     4741 ggtcctcccg gagcgggaga gcggtcgcga cgaggacacc gcgccagggc cgcggcgcgg
     4801 cggcggccgc cgcggccgga gcgggagccg gggagccggc agccggggag ccgggtgcgg
     4861 ggaagtccgg ggcggatgcg gatgcggacg cggtcatgag gcgggcccct tccgtgggag
     4921 gacatacggg atgtgcggga tgtgcgggat gtgcgtggcc cgagcgggac gcgcaccgta
     4981 cgaggtgtga cattgcactg tcatgagctg tccatggggt cgtccggcgc ggccagcacg
     5041 cccaggggca cgggccgggc cagcagccgt cgggccggcc ggagttcgca gcccgcgacg
     5101 cccgcgacac cgggctcgca catccttccc tggcaccagc ccatgccggc ccgggtcagc
     5161 agtttcacgg tgcgcagatc gcccgcgccg agcgtgtcca cggcctcgcg gacggcgccg
     5221 gcggtgacct cctcgcagcg gcacaccacc gtcccgtcgg tcacctgctc cgtccaccgc
     5281 acgggccggg cggcgtacgc ggcgtccagt gccgcgaaga acgcccgcgc gcgggtacgc
     5341 gcccgggcgg ccggagccca ggcgcgcggg tcggggacgg cgcccgtcag ccgggccgct
     5401 gccgatcgcc cggcgatgtg gccctcggcg agcgacagtg ccgcgccgcc gatgcccgtg
     5461 gtctcacccg cggcccacac gccggggacg tcggtgcgct gttcctcgtc gacgacgacg
     5521 cgcggcccgt ccagacggca gccgagcccg tccgcgaggt cggtgtgcgg cagcagcccg
     5581 tggccgacgg cgagggtgtc gcagacgacg cgccgccgcg tgccgggcac cggccggccc
     5641 tcgcggtcga gcgcggcgac ggacacggcc tccagacgct cgtcgccgtg cgccgccagc
     5701 accgtgtgcc gggtcatcac cggcacacga cggcccagga gccgggtcgc gtaggcgatt
     5761 gcctccgtga ccttcgcggg ggcggctgcc caggtcccgg cgcggcgcac gaaaccggcg
     5821 gcccccgccg actcgaccag ggcgacgacg tcgacgcccg cggccgcgag gccggtggcc
     5881 acgggcagca gcaggggccc ggtgccggcc acgaccgcgg tgcggccggg cacggcgagc
     5941 ccgcccttga gcagggcctg ggcgccgccc gcggtgacca cgccgggcag cgtccagccg
     6001 gggaacggca ggaccttctc gtagccgccc gtcgccagca gaactccgtc gacgcgtacc
     6061 gcgacggact cctcctggtc gggaccggcc agcgcgtgca cggtgaatcc gtgtgaagcc
     6121 cgttccacgc accacacatg acggtcactc aggtacgtga cccggccgat gccgagctgg
     6181 gcgtcgagcc cggcgcgcag tcgctcccag gtgcgcgacc ggtggcggag ggccggtggg
     6241 cgccgggcgg ccgttcccgg tgcgggctgc cggtggaact gtccgcccgg cccgggctcg
     6301 gagtcgacca gcaccacctg tacgccgtgc gcggcggccg ccagggcggc ggcgaggccg
     6361 gccgggcccg cgccgatcac cgcgagtacc ggacggtcct cagtcatcgt gacccgtccc
     6421 gtgctgggtg cggacctcgg cgcccggcac cgccggcacc aggcaggcgc gtcggccggg
     6481 acggccgtcg acggtgacca ggcagtcgaa gcacacgccg atcccgcaga agacgccgcg
     6541 cgcccgcccc gaaccccggg tggtgcgcca ggccgtgacg ccggacgtcc acagcgcggc
     6601 ggcgatcgtc tgcccgggca gtgcctcgat ctcccggccg tcgaaggtga gggtgaacgg
     6661 gtcgccgggg tggtcccgga gcggctcgcg cggattcacg gggtgtcctc ctcgtcatgg
     6721 cggtcgtcgt cgaagcggtc gggccgcagc ggtgtcaggt cgaggtccgg gcgctcgccg
     6781 gcgaggacct gcgcgatgag gtggccggtg ccggtcgcga gcccgatgcc cgcaccctcg
     6841 tgcccgcagg cgtggaagag ccccggcacc cggggatcgg gtcccacggc cgggagatgg
     6901 tcgggcaggt acgggcggaa gccgaggtac gtgcgcatgg cccggacccg ggccaggaag
     6961 ggaaagaggc gggtcgcgcc cgccgccagc gcccgcacga ccggcaggga gaaggaccgg
     7021 tcgaaaccca cccgctcacg gctcgcgccg atcagcaccg gtccggccgc ggtcccctcg
     7081 acgaccggtg aggtctgcaa cgcggcggaa tcgctcgcca cgtcggccac gtagtcggcg
     7141 gcgtacacct tgtgccggat ccggcgcggc agcggctcgg tgaccaggac gaagccccgg
     7201 cgcgggagca cgggcagccc gaccccggcc agcgcggcga cctcggcacc ccaggtcccc
     7261 gccgcgttca ccacggccgg ggcgaggatg tcgccgcggt ccgtgcggac gccccgcacc
     7321 gcgccggtgg tgtcgcgcag cacccgcgtc accgtgcggc cggtctccag gcgcgcgccg
     7381 ctcgcccgga cgagacgggc cgcggccagc gtcggcatga cctgggcgtc ctgcggatac
     7441 agcacaccgc ccgcgaggcc ccgcgccagg tccggctcca ggtcgtggag ttcgtcggcg
     7501 ccgaccggta cggccgtcac cccggccgcg cgctgtccgg ccgcgagacc ggtgagggag
     7561 ccgagcgcct cgggcgtggc ggcgacgacg agcccgccct tggcctcgta ctcgacggcc
     7621 ggacccgtct cctcggcgag ctcgccccac aaccgggccg acagcagggc gagtcggagt
     7681 tccggcccgg gctccttgtc ggacacgagc aggttgccct caccggcgcc ggtcgtgccc
     7741 ccggccaccg gaccccggtc gaccaggatc acgtccagcc ctgctcgggc cgcgtacagg
     7801 gcgcaggcgg cgcccaccat gccggcaccg acgaccacga cgtcaccaca ggatcgcttg
     7861 gtcacgacag taatatgtca catggcgccg atgccgaaaa gaaggccgac ccggggccga
     7921 cccgggaaaa tgcgaggtcg tgcgggcccc ggccggggca tcatggcggt cccgccaccc
     7981 ggccgtcggg ggcgggacca gcaccaggga acgaccgggg gcacctgtga ccgtcaccaa
     8041 catcctgctg tccggcattg tcggctcgac cgcctacgga ctcgcgcgcg aaggctccga
     8101 cgtggaccgc ctcggcatgt tcgccgcccc caccgagcaa ctgcacgggc tgcaccagcc
     8161 gaaggagtcg tacgtcagca cggcgcccga ccgcaccctg cacgaggcgg ccaagtggtg
     8221 caggctcgcc ctcggcggca accccacggt gatggaactc gtctggctgc ccgacgagtt
     8281 gtacgaggtc cgcaccccgc tcggcgacga actcatcgcc ctgcgcgaga cgctgctcag
     8341 cgcccgtcgg gtgcgcgacg cctacctcgg ttacgccacc cagcagttcc gcaagctcca
     8401 gaaccgcacc ggcggcaccc cctccgagga cacccgcggc cggatcgcca agaacgcccg
     8461 ccacctcaag cggctgtgca cccagggcta cgagctgtac tccaccgggc ggttgacgat
     8521 ccgcgtcgac gacccggaga gctaccacag gttcggtgag caggtcgccg acgaccccga
     8581 ggccgcgctg ccgctgctgc gcggcttcga ggaggcgttc gccgggaccc gcagcgtgct
     8641 gcccgaacag cccgacgaga cggcggcgga cgcctggctg cggcgggtac gcgaggagtt
     8701 ctacccggcg gcgcggacca cgcccgtgtg atccgcccgc caggcggcgc gcaccccgcg
     8761 cccgccgccg tgcaccccgt gtccgccgcc gcgcccgggg acacccccgg cgcgggtgcg
     8821 acaaaatcat gtgcggtgcc cgcgccggcg cccctacact cgccgtcacc gagcgcggcc
     8881 cacgagtggg gcggcgcgta ccgagacgaa cgaggggagc ccggccatgc gtgaccacac
     8941 cgctgccgtc gctccccggt cccgctacga ggtgatcctc gtgtcttcgt ccgtccggtg
     9001 ctcgctgcgc ggccggcacc gggcctcctg acgaaggtcc gaccacagca gacccacccg
     9061 tgacctggtg cgtcgccctg cgcggcgcac cgacgccgac tgcacgccgg cgtccgtcac
     9121 gtgtcacgac ctcgtccggg aaatcgcgcc gcccgttccc gcaacacccc gaaaggccaa
     9181 gggccgtgcg caacaacctc gaccgtcagc tctccaccgt gcgcaacctg ggcatcctcg
     9241 cccacgtcga cgcgggcaag accaccgtca ccgaacggat cctgtacgcc accgggacca
     9301 cgcacaagcg cggcgaggtc catgacggca cgaccgtcac cgacttcgac ccccaggagc
     9361 gcgaccgtgg catcaccatc ttcgccgcgg ccgtcagctg cgagtgggcg gggcaccgca
     9421 tcaacctgat cgacaccccc gggcacgtcg acttcgccga cgaggtcgaa cggtccctgc
     9481 gggtgctcga cggcgcggtc gccgtcttcg acgccgtcgc cggcgtggag ccgcagagcg
     9541 agtccgtgtg gcgtcaggcg gaccggcacg gcgtgccgcg gatcgcgttc gtcaacaagc
     9601 tggaccgggt gggcgccgac ctcgacaccg cggtggcgtc gatccgtgac cgcctccatc
     9661 cggttccgct ggtcgtccac ctgccgatcg gcgcggagga cggcttcacc ggcgtcgtcg
     9721 acctgctgag gatgcgcgcg ctgacctgga gcgacggcag cgacgcggcc gtcgaggatg
     9781 acgtcccgga cgaccttctg gcggaagccc tgcggcggcg ccggctgctg gaggagaccg
     9841 tcgcggagcg gcatccggcc gccctggagg agttctgcga ccgggagacc ctctccgccg
     9901 ggaccctggc ggccgccctg cgcgacctga cccggacggg cgacggcgtg gtcgtgctgt
     9961 gcggttcggc ctaccgcaac cgcggcgtcg aaccgctgct cgacgccgtc gtcgcctatc
    10021 tgccgtcccc gctggacgtg ccgcctgtac gcggtgtgtc tggggctgcg ggcggtgtgc
    10081 ccggcggtga ggagcggacg gccgacccgt ccgcgccgtt cgccgccctc gcgttcaagg
    10141 tcaccgccac ggcgaccggc cgtctcaccc atctgcgggt gtactcgggc acgatcggga
    10201 agggagacgc ggtatgggac tcgggcgcgg gacgcaccga gcggatcggc cggatcctgc
    10261 gggtgcaggc cgaccggcac gccgggctgg agcgcgcggt cgccggggac atcgtggccg
    10321 tggtcgggct gaagtcggcc cgtgcgggct cgaccctcag cgcgccggac gcacccgtcg
    10381 tcctcgaacc gcccggcgtc gccacgcccg tggtctccgt cgcggtcgag gcgcgcaccg
    10441 gcggggacac cgaccggctg gccgtggccc tggcccggct ggcggaggag gatccgtcgc
    10501 tcgtcgtccg gaccgacccg gagaccggtc agaccgtgct gtcgggcatg ggtgaactgc
    10561 acctggaggt cgcggtggag aagatccgcc gcggtctcgg cctggacgtc caggtgggcc
    10621 gccccagggt gacctaccgc gagacggtcg gccgcggcgt gtccggcgcg gtcttccggc
    10681 acgtcaaaca ggacggcgga gcaggccagt tcgcgcatgt cgtcctcgac gtcgagccgc
    10741 tcggcgcgga cgaggcggga gacggcgcgg cggccgggtt cgccttccgg tccaccgtcg
    10801 tcgggggccg ggtgccgcac gagtacgtcc gcgcggtcga ggccggctgc cgggacgccc
    10861 tcgccgaagg acccatcggc ggccatccgg tcaccgggtt gcgggtcacc ctgacggacg
    10921 gggcgaccca tgtgagggac tcctccgaga cggccttccg cacggccggc cggctcgggc
    10981 tgcgagaggc gctgcgcgcc tgtgcgatgg tcctgctgga acccgtcgtc gaggtcgcgg
    11041 tcaccgtccc cgaggacgcc gtcggcgggg tcctcggtga cctggccgcc cggcgcggcc
    11101 gggtcaccga ctcggccgtc cgcggcggct cggcggtggt caccgcgacc gtgccgctgg
    11161 ccgaactgtt cggctatgcg acccggttgc gcagccggac gcagggccgg ggcaccttct
    11221 cggtccgccc gacgggctac gccccggccc cgacaccggt gacgccccgc tagccccgct
    11281 ccgccgcccc acggccccgc acggccgagg gagggcggcc gtgctcacgg tctccgaccc
    11341 cggaggccat gggcccggcg gtcacttctt gccgacgggc ggacccgcgg ggtcggggtg
    11401 caggcccagg gccggtgccc ggcgtcacgg aggtgtcggc cgagccacgg ccctgcccgt
    11461 cgctccgtgt tgacgggcca gggcgcagat tccgggccgg gtccggccgg cgcccggacc
    11521 cgtgggtccc gggtcgcagg tccccgaccc ggtccacagc cggtcgtagg gcaggcagcg
    11581 ggtgcgcccg tcgcctcgcg cggacgggcg gcgggcccgc ggtcccgggg accggccccc
    11641 gaggtccagg ggccgcggcc cggtactcac gaaggcgtcg gtcgagcctc ggacccgccc
    11701 gtcgctccgt gcggacgggt gggcccgtgg ccgtgcttgc gggcgcgggg attccggccc
    11761 tcgggccccg gccacggcat ggcccccgtg gccgtcgggg acctcaccga gggcggcggg
    11821 ccggtggacg ggtcaggggt aggacaccac ggtcgagggg accgtggagg tgccggaggt
    11881 cggggccccg aggtcgttga tcacgtgctc gtactggccc tggccgccca gcgagaccac
    11941 cagcaggtcg tggaacttca cccccggcac gtccggggcg gcgaacccgt ggtgctggac
    12001 gatcgtcggg tcgacgttgt agaagcagta gctgccgagt ccccagccct cgtgcgtggt
    12061 gacgcccgcg ccgaccttgt acgcggcgta ccccttgacc gggccgttct ggatcgcggc
    12121 ctggtccggc gcgtcgtagg ccttctcgtt ctggaagaag atcgtgcggc cgcgctgtcc
    12181 gttccactgc acgtcgtact tgttgaagtg ctccacgaac aggcccgtgg ccagcacgtc
    12241 gtcaccgttg acgacgaccc cgtagtcggc ccggttggtc tcccacccga cgccctcccc
    12301 gtggtcggcg cgccagaccc aggtgtggtc gacgatcgtg tgacggttgt tgaccaccat
    12361 gctggtggtg gcccgtccgg cgccggcccc gccgatccgg acgaacacgt cctggacggt
    12421 ggtcgggtcg gcgctgtggt cgcgccgtgc cccgggcgga ccgatctcca gcagggtcct
    12481 ggagttgacc ggtcccgcgt cgacgaggaa gcccgcgagg cggaccccgt cgacgtcggc
    12541 caccttcagc gcggtgacgc cgttgtcggg gatcagggtg gcgtacccga gcccgaggac
    12601 gaccgtgccc gccctgttca cccggaccgg ccggtcgaga tggtagacgc cgggcgtcag
    12661 cagcaggtgg aggccctgcg cgagcgcctg gttgagcgtg gccgcggaca ctcccggccg
    12721 ggcgacgtag aaccgcgaca ggggcagcga actgccgcgc ggggtgccct tgccccacgt
    12781 gaccccgcgg gcgcctgtcc gcttcgccgg caggaacacc cggaagccgc cgcggccgtc
    12841 gacgtacagg aacggcttct cccgg
//
