LOCUS       NZ_BMSI01000007        11410 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence07, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000007
VERSION     NZ_BMSI01000007.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 419478)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 69323
            Orig. end                         :: 80733
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11410
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11410
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11410
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6410](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6410)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(594..609)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.99"
                     /tool="antismash"
     gene            607..1338
                     /gene="hemQ"
                     /locus_tag="IE189_RS18470"
                     /old_locus_tag="GCM10010496_37760"
     CDS             607..1338
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="IE189_RS18470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37760"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_189922964.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRVPNKGKLAKDLNEVIRYTLWSVFRLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTVRGTYDVSGLRADADLMIWWHAETADQLQEAYNLFRRTR
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGYPDVRANTVASFSLGDYEWMLAFEADELYRIVDLMRHLRASEARMHVR
                     EEVPFYTGRRKSVADLVAGLA"
     gene            complement(1363..2961)
                     /locus_tag="IE189_RS18475"
                     /old_locus_tag="GCM10010496_37770"
     CDS             complement(1363..2961)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (114-488). E-value:
                     5.3e-24. Score: 77.1. Matches aSDomain:
                     nrpspksdomains_IE189_RS18475_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073952.1"
                     /locus_tag="IE189_RS18475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37770"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_189922966.1"
                     /sec_met_domain="PF00561 (E-value: 5e-29, bitscore: 96.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALHSAAGALLLTALSAVPAGAVPADGAAGAPGLSRSPGVVL
                     AAARAEAKGVDFTKCPGGQDLPGSMQCGKVSVPLDYADPSGRQIELTVSRVRATQRDPH
                     NSKRRVPRQGALVFNPGGPGGSGLYFPLIGLLPEWKRIGAAYDLVGYDPRGVGRSAPLS
                     CQDPKRFFKGPAPAPVHPSESYKRERIAQAKAYARGCAKRSGSALRHYNSINNARDLDV
                     VRAALGEDRLTFMGASYGTYFGAVYATLFPSHVRRMVFDAAVNPDPAQIWYRNNLDQSA
                     AFESRWADFREWIARHDDVYGLGRTADAVLRSYERARARLAAEPAGGKVGPGQLQSAFL
                     QAGYYDDYWPARAQALSEYLKGDPKQLIAQAGPVREAAAEAENANAVYTAVECNDASWP
                     TDWKVWDRDNTRLARVAPFETWDNVWANLPCAYWPAPRQRPVDVRTGPGELPPTLILAA
                     ERDAAAPYAGALEMHRRLSGSVLVTERDAGTHGIAGGPNRCVNGHLDAYLLEGRLPARR
                     AACAAHAEPEPTAAR"
     aSDomain        complement(1498..2619)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS18475_Abhydrolase_1.1"
                     /evalue="5.30E-24"
                     /label="IE189_RS18475_Abhydrolase_1.1"
                     /locus_tag="IE189_RS18475"
                     /protein_end="488"
                     /protein_start="114"
                     /score="77.1"
                     /tool="antismash"
                     /translation="ALVFNPGGPGGSGLYFPLIGLLPEWKRIGAAYDLVGYDPRGVGRS
                     APLSCQDPKRFFKGPAPAPVHPSESYKRERIAQAKAYARGCAKRSGSALRHYNSINNAR
                     DLDVVRAALGEDRLTFMGASYGTYFGAVYATLFPSHVRRMVFDAAVNPDPAQIWYRNNL
                     DQSAAFESRWADFREWIARHDDVYGLGRTADAVLRSYERARARLAAEPAGGKVGPGQLQ
                     SAFLQAGYYDDYWPARAQALSEYLKGDPKQLIAQAGPVREAAAEAENANAVYTAVECND
                     ASWPTDWKVWDRDNTRLARVAPFETWDNVWANLPCAYWPAPRQRPVDVRTGPGELPPTL
                     ILAAERDAAAPYAGALEMHRRLSGSVLVTERDAG"
     gene            complement(3093..3869)
                     /locus_tag="IE189_RS18480"
                     /old_locus_tag="GCM10010496_37780"
     CDS             complement(3093..3869)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997028.1"
                     /locus_tag="IE189_RS18480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37780"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_189922968.1"
                     /transl_table=11
                     /translation="MIWVLLLPLAWALAGAACTRLCLAAVRAASVDDDTGRGHDLTLYE
                     AAFLSGGPGRVADLTMVSMARQRRLLLARTGWATVVDPRGRDEMERSVIGAIGPAGQSR
                     IAPARAAAAAADAVGGLADRLVRAGLAVPDGSRTTVAAGVRQVRAAAAAVLALGATALL
                     APGPSDMPRDLVALWFALPLALTLGCLAIARVEAHPYTRWASPAGQRLLAALTRHTGAP
                     ADDGTCLTSVAVRGIRAVREPDLRAAFAHREQDTRG"
     gene            complement(3970..4758)
                     /locus_tag="IE189_RS18485"
                     /old_locus_tag="GCM10010496_37790"
     CDS             complement(3970..4758)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073954.1"
                     /locus_tag="IE189_RS18485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37790"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_189922970.1"
                     /transl_table=11
                     /translation="MRPRPPIQGRGIFSGTGLILTGLAATLAALVFPIWSYADRSGTGV
                     DVLNAETVSTQYGPLSALDREFVTKVRLAGLWELPAGRQAQAKGTTEAVRTAGAHLVEG
                     HTFLDERVRAVASRLGLALPNEPNDQQRGWLSELDAAQGVAYDREFANILRLAHGRVFS
                     VVAQVRAGTRNSLVRGLADDANTTVLDHIKILEATGYVDFDALARDLAADSTPPITRAP
                     APPGPTTNPAPAVPAESASASAASPTYPLPPAAASPPPGS"
     gene            complement(5001..6410)
                     /locus_tag="IE189_RS18490"
                     /old_locus_tag="GCM10010496_37800"
     CDS             complement(5001..6410)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125240.1"
                     /locus_tag="IE189_RS18490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37800"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_189922972.1"
                     /sec_met_domain="DUF692 (E-value: 7.2e-96, bitscore: 314.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADTVERMPGIDWVEAVAENVCPGHLPDSLLR
                     LRERGVTVVPHGVSLGLGGAERPDAGRLTALAERAEVLGSPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTRDALRVLCENVRIAQDALPVPLAVENIAALVAWPGEEMTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNRGEEPVKALAELPLEAIAYVHVAGGFERDGVWHDSH
                     AHPVPGPVLDILTDLASRTALPGVLLERDENFPGPGELERELDAVREAVGTGATARGTT
                     DGRERPAREGTRSGGAASEGAPGEGAGGEGAPGEGAGGGGSVPPAAEAAPAAPDEAARQ
                     RLALAEAALLSALVAGTPVPEGFDRVRLGVQARALAAKRADVVAKVAPELPLILGSGYR
                     TAFVAYAQGHPMRGGYRQDALDFAEELLMGAHPAQGRARRELRRWWLDRSGPAPRPASR
                     LGQVGRSLIRR"
     gene            complement(6550..7308)
                     /locus_tag="IE189_RS18495"
                     /old_locus_tag="GCM10010496_37810"
     CDS             complement(6550..7308)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993753.1"
                     /locus_tag="IE189_RS18495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37810"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_189922974.1"
                     /transl_table=11
                     /translation="MDLPPGRLEDVTSDPAVPGDSDSPFRSEPSTRDEAPQFVLPLVVR
                     IERAAPPARTDALETAARAVLTLLADERALGDGEWAAAVRDWQDARIRKVVRRARGAEW
                     LRAEALPGITVTGKAAQVRVFPPVPLDGWPRELAKLQVSGTDLDDPEPPPGVDLTAPVI
                     WQNPEVTMSAGKAMAQAGHGAQLAWWELSDEERTAWREAGFPLAVRTAGPRHWGELTAS
                     GLPLVRDAGFTEIAPGSCTVVADHPALRNR"
     gene            7336..8193
                     /locus_tag="IE189_RS18500"
                     /old_locus_tag="GCM10010496_37820"
     CDS             7336..8193
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675661.1"
                     /locus_tag="IE189_RS18500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37820"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_189922976.1"
                     /transl_table=11
                     /translation="MPGELRLEGVGRRYGVRGPWVLRDVHLHPAPGTLTRVGGPNGTGK
                     STLLRLLARLDAPTEGRVTGTPRTAYVPERFPAALPFTALRYLTHLGAVHGLSRTAAER
                     AAGYWLDRFGTGGHAHTPMSRLSKGGSQKVAVAQALLAEPELLVLDEAWTGLDTDARAE
                     LDRAVAERTAAGSAVVFVDHDPRRLAGLADAEYTVGDGTLRRRTAPEPAAAPVGPHLTV
                     EVRGPAGELPAQALRLAASAEETADGTHRLTVPVSHSDVLLRVLLTARPPWHVLSVSPR
                     GGAR"
     gene            8190..8861
                     /locus_tag="IE189_RS18505"
                     /old_locus_tag="GCM10010496_37830"
     CDS             8190..8861
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="IE189_RS18505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37830"
                     /product="ABC transporter"
                     /protein_id="WP_189922978.1"
                     /transl_table=11
                     /translation="MTALLRYQSALLVRSQRWLPPFLLYAVFLAVGVQAGEPLLDSLGY
                     TAAFLLPVAAWLVRICAGGEPPAARSVVAAAAGPARAHLACVLVATGAAAAFGTAATVV
                     VTLVSDPVGSDHRTRVPVLGAAGAGLLAVLTCALLGAAVGALTAWPVLRSPGRGVPALL
                     LAVLLALVGTGSPAQAAVSGLVGGSRSATVRPPVLPLVAAALVCAAATALACVLTSRRA
                     P"
     gene            complement(8898..9089)
                     /locus_tag="IE189_RS18510"
                     /old_locus_tag="GCM10010496_37840"
     CDS             complement(8898..9089)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523443.1"
                     /locus_tag="IE189_RS18510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37840"
                     /product="hypothetical protein"
                     /protein_id="WP_189922980.1"
                     /transl_table=11
                     /translation="MSDDDGDRDYCLIDATRPPRAEGPPYAECVLCRRPTEYPESYKGI
                     TMCPVCEWQEAQRTACSG"
     gene            complement(9127..10047)
                     /locus_tag="IE189_RS18515"
                     /old_locus_tag="GCM10010496_37850"
     CDS             complement(9127..10047)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325985.1"
                     /locus_tag="IE189_RS18515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_37850"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_189922982.1"
                     /transl_table=11
                     /translation="MITPVRRVAALCALAAALAPLTACGTAQDTRAVRTTDSATAPAAG
                     ARNAAPTPSRTPAAPSRTPAAPSRTPTLAPGPAGLTPVFRNGPRTAGKTVALTFDADMT
                     ADQGPRAAAGEHFDNPGLITALRTLKVPATVFMTGRWAEEYPDQARSLGRDPGFEIANH
                     SYSHHAFTDDCYGLPTVPEDAMRTDVERAYTAFRKAGVPSAMPYFRFPGGCYDQRALRA
                     VSGLGVTAVQWDVVSGDAFATDADAVARQVLDGVRPGSVVVMHCTRSAAPTTEQVVRTV
                     VPELRRRGYRFVKVSELIATAGGRR"
     misc_feature    complement(10078..10091)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.08"
                     /tool="antismash"
     misc_feature    complement(10502..10516)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 cgccctgctg gccgcggagt ccccggcggc ggccgccgcc ctgcgcacgg tggagtacgc
       61 ctccatggcc ctggtcaccc tcgcctaccg ccgcaccggc acggagctgc ccgagggcag
      121 cggcttcctg gtcccgcccg tcgacggacg caccatcaag gcgtccacct tcgcctcccg
      181 gaagtggggc tggatcgcgg acgagaaccc ggacaccgtc gtgctgcgca cctccgtggg
      241 gcgctacggc gagagcgaga tcctcgaacg ggacgacgcc gacctcgtcg ccgtctcccg
      301 acgcgacctg cgcgccgcca ccggcctgga cgccaccccc gtcgagacgc gcgtcacccg
      361 ctggaccgac ggcctgcccc agtacccggt cggccaccac gcgcgcgtgg cccgcatccg
      421 ggagcacgtc gccgcgctcc cggacctcgc ggtgtgcggc gcgcagtacg acggcgtcgg
      481 catccccgcc tgcatcgcga gcgcgtacgc cgccgtcgac cagctgggcg gcgacctccg
      541 cgccgtccgc gcgctcgcgg ccgacccggt gcagagtctg cacggcggag cgggagaatg
      601 aggagcatga gtgacgacgc ccccaccacc gagtccggca gggtcccgaa caagggcaag
      661 ctggccaagg acctcaacga ggtcatccgc tacacgctgt ggtccgtctt caggctgaag
      721 gacgtgctcc ccgaggaccg cgcgggctac gccgacgagg tccaggagct gttcgaccag
      781 ctcgccgcca aggacgtgac ggtccgcggc acgtacgacg tgtccggtct gcgcgccgac
      841 gccgacctca tgatctggtg gcacgccgag acggccgacc agctccagga ggcgtacaac
      901 ctcttccgcc gcaccaggct gggccgcgcc ctggagccgg tctggtcgaa catggcgctg
      961 caccgcccgg ccgagttcaa ccgctcgcac atcccggcgt tcctcgccga cgagacgccc
     1021 cgcaactacg tgagcgtgta ccccttcgtg cgctcctacg actggtacct gctgccggac
     1081 gaggaccgtc gccggatgct cgccgaccac gggaagatgg cccgcggcta ccccgacgtc
     1141 cgcgccaaca ccgtcgcctc gttctcgctg ggcgactacg agtggatgct ggccttcgag
     1201 gccgacgagc tgtaccgcat cgtcgacctc atgcgccacc tgcgggcctc cgaggcacgt
     1261 atgcacgtcc gcgaggaggt cccgttctac acgggccgtc gcaagtcggt ggccgacctg
     1321 gtcgccggac tggcctgacg cccccgcgag ggggcgcggg cctcagcgcg cggcggtggg
     1381 ttccggctcc gcgtgcgccg cgcaggcggc gcgccgggcc gggagccgcc cctccagcag
     1441 atacgcgtcc aggtgaccgt tgacgcaccg gttgggtccg cccgcgatgc cgtgggtacc
     1501 ggcgtcccgc tccgtcacca ggaccgagcc cgacagccgc cggtgcatct cgagggcccc
     1561 ggcgtacggt gcggcggcgt cccgctccgc cgccaggatc agcgtcggcg gcagctcgcc
     1621 cggcccggtc cgcacgtcga ccggccgctg ccggggcgcc ggccagtacg cgcagggcag
     1681 gttcgcccac acgttgtccc acgtctcgaa cggcgcgacc ctggccagcc gggtgttgtc
     1741 gcggtcccac accttccagt ccgtcggcca ggacgcgtcg ttgcattcca cggccgtgta
     1801 cacggcgttg gcgttctccg cctcggcggc ggcctcccgc accggcccgg cctgcgcgat
     1861 cagctgcttc gggtcgccct tgaggtactc cgacagcgcc tgtgcccggg ccggccagta
     1921 gtcgtcgtag tacccggcct ggaggaacgc gctctggagc tgtcccggcc ccaccttccc
     1981 gccggccggc tcggcggcca gccgggcgcg cgcccgctcg tagctgcgca gcaccgcgtc
     2041 ggccgtgcgc ccgaggccgt acacgtcgtc gtgccgggcg atccactccc ggaagtccgc
     2101 ccaccggctc tcgaacgcgg ccgactggtc gaggttgttg cggtaccaga tctgtgccgg
     2161 gtccgggttc acggccgcgt cgaacaccat ccgccgcacg tgggagggga acagcgtcgc
     2221 gtagaccgcc ccgaagtagg tgccgtacga cgcccccatg aacgtcagcc ggtcctcgcc
     2281 gagcgcggcg cgcaccacgt ccaggtcacg ggcgttgttg atcgagttgt agtgccgcag
     2341 ggcgctgccc gaccgcttcg cgcagccgcg cgcgtacgcc ttggcctgtg cgatccgctc
     2401 ccgcttgtac gactccgagg ggtggaccgg agcgggcgcc ggccccttga agaagcgctt
     2461 ggggtcctgg caggacagcg gtgccgaacg gccgaccccg cgcgggtcgt agccgacgag
     2521 gtcgtaggcc gcgccgatgc gcttccactc cggcagcaga ccgatcaggg ggaagtacag
     2581 gcccgagccg ccgggaccgc ccgggttgaa gaccagcgcg ccctgccggg gcacccgccg
     2641 cttgctgttg tgcgggtccc gctgggtggc ccgcacccgg ctgacggtca gctcgatctg
     2701 cctgccgctc gggtccgcgt agtcgagcgg cacgctgacc ttgccgcact gcatgctgcc
     2761 gggcaggtcc tggccgccgg ggcacttggt gaagtcgacg cccttcgcct cggcccgggc
     2821 ggcggcgagc acgacgccgg gcgagcggga gagtccgggg gcgcccgcgg cgccgtccgc
     2881 cgggacggcg cccgccggga ctgcggagag ggcggtcagg agcagggctc ctgcggccga
     2941 atggagggcg gctgctctca tcgggagtcc cttcggtgca cggcggcgac aaaggggatg
     3001 tttggttcgg tcggagggga aggcaagcac caccggcggg tgtcggcgcc aatgcctcct
     3061 gtgcgccccc cggcggtcgg gcgcgccctg cttcagccgc gcgtgtcctg ctcacggtgt
     3121 gcgaaggccg cccgcaggtc cggttcgcgc acggcgcgga tcccgcgtac ggcgacggag
     3181 gtcaggcagg tgccgtcgtc cgccggggcg ccggtgtgcc gggtcagggc ggccagcagc
     3241 cgctgtccgg cgggggaggc ccagcgcgtg taggggtgcg cttcgacccg cgctatggcc
     3301 agacagccca gggtgagggc caggggcagc gcgaaccaga gggcgaccag gtcacggggc
     3361 atgtccgagg ggccgggggc cagcagtgcg gtggcgccca gggcgaggac ggccgcggcg
     3421 gcggcccgta cctgccgcac cccggcggcg acggtcgtac gggagccgtc gggcaccgcg
     3481 aggccggcgc ggaccagccg gtcggcgagg ccgcccaccg cgtccgcggc ggccgcggcc
     3541 gcccgcgcgg gcgcgatacg cgactgtccc gcggggccga tcgccccgat gaccgaccgc
     3601 tccatctcgt cgcgcccgcg ggggtcgacg acggtcgccc agccggtgcg ggcgagcagc
     3661 agccggcgct ggcgcgccat cgagaccatg gtgaggtcgg cgacccggcc ggggccgccg
     3721 gacaggaagg cggcctcgta gagcgtcagg tcgtgccccc ggcccgtgtc gtcgtcgacg
     3781 gaggccgccc gcacggcggc gaggcacagc cgtgtgcacg ccgcgcccgc gagggcccag
     3841 gccagcggca ggagaagcac ccagatcatg cggtgtttct atgtcatgtg caccaccggg
     3901 cgccacggtc tgttcatcat ccggaccggg cgcgcgggac ggccggctcc ggccgccccg
     3961 gcctcgctct caggagcccg gcggtgggct ggccgcggcg ggcggaagcg gataggtggg
     4021 ggacgccgcc gacgcggacg ccgactccgc cggcacggcg ggcgcggggt tcgtcgtcgg
     4081 gcccggcggg gcgggggcgc gggtgatcgg cggtgtgctg tcggcggcca ggtcccgggc
     4141 cagggcgtcg aagtcgacgt acccggtggc ctccaggatc ttgatgtggt ccagcacggt
     4201 ggtgttggcg tcgtcggcga gcccgcgcac cagtgagttg cgggtgccgg cccgcacctg
     4261 ggcgacgacg gagaagaccc gcccgtgggc caggcgcagg atgttggcga actcgcggtc
     4321 gtacgccacg ccctgagccg cgtcgagttc gctcagccag ccccgctgct ggtcgttggg
     4381 ctcgttcggc agcgcgaggc ccagccggga ggcgacggcg cgcacccgct cgtccaggaa
     4441 ggtgtgcccc tcgaccaggt gcgcgcccgc cgtccgcacg gcctcggtcg tgcccttggc
     4501 ctgcgcctgc cggccggcgg gcagctccca cagccccgcc aggcggacct tcgtgacgaa
     4561 ctcccggtcc agcgcggaca gcggaccgta ctgcgtcgag acggtctcgg cgttcagcac
     4621 gtccactccg gtgcccgacc ggtcggcgta cgaccagatc gggaagacca gggccgccag
     4681 ggtcgccgcc aggccggtga ggatgagccc ggtgccactg aagatgcctc tgccctggat
     4741 cgggggtcgg ggccgcatgg ggtgcctcct gctcgcggca ctgcacgcta gtgcgcttcg
     4801 gttgcggaac tggcatatta ctgcgcctgc cccggcaacc cccttacagg ggcaaagagt
     4861 tgaaataccg ccaaaccggt cggagggtgg gcggtaagag ggtccgcaaa aggggcgaaa
     4921 ggcggaggaa cggcggggga agcggtcgcc gtgtgcccca tgcggggccc ggtgttaacc
     4981 ccggcacatt cgggccgccc tcaccgccgg atcaacgacc ggcccacctg ccccagccgc
     5041 gacgccggtc tcggcgcggg acccgagcgg tccagccacc agcggcgcag ctcgcgtcgg
     5101 gcccggccct gcgccggatg cgcgcccatg agcagctcct ccgcgaagtc gagggcgtcc
     5161 tgccggtagc cgccgcgcat cggatgcccc tgcgcgtagg ccacgaaggc ggtccggtac
     5221 ccgctgccga ggatcagcgg cagttccggg gccaccttcg ccacgacgtc cgcccgcttg
     5281 gccgccagcg cccgcgcctg cacgccgagc cgtacccggt cgaagccctc gggtacgggc
     5341 gtcccggcga ccagcgcgga cagcagggcc gcctcggcga gggccaggcg ctgccgggcc
     5401 gcctcgtcgg gagccgccgg tgccgcctcc gcggcgggcg ggaccgagcc gccgcccccc
     5461 gctccttcgc ccggcgcccc ttcgcccccc gctccttcgc ccggcgcccc ttcgctcgcc
     5521 gccccgccgc tccgtgtccc ctcgcgcgcc ggccgctccc ggccgtccgt cgtgccgcgg
     5581 gccgtcgcgc cggtcccgac cgcctcccgg accgcgtcca gctcccgctc cagctcgccc
     5641 ggcccgggga agttctcgtc ccgctccagc agcacacccg gcagcgccgt ccgggacgcg
     5701 agatcggtca ggatgtcgag gaccggcccg ggcaccgggt gggcgtgact gtcgtgccag
     5761 acgccgtccc gctcgaagcc gcccgcgaca tggacgtagg cgatggcctc caggggcagt
     5821 tcggcgagcg ccttgacggg ctcctcgccg cggttgacgt ggttggtgtg caggttcgcc
     5881 acgtcgatga gcagccgcac gccggtgcgg tcggccagct cgtacaggaa ctgcccctcg
     5941 gtcatctcct cgcccggcca ggcgacgagc gcggcgatgt tctcgaccgc gagcggtacg
     6001 ggcagcgcgt cctgtgcgat ccgcacgttc tcgcacagca cgcgcagggc gtcgcgggta
     6061 cgcgggacgg gcagcagatg tccggcctcc agccgcgggg acgccgtcag ggggccgccc
     6121 gcccggacga acgcgatgtg ctcggtgacc agcggcgagc ccaggacctc ggcccgctcg
     6181 gcgagggcgg tcagccggcc cgcgtcgggc cgctccgcgc cgccgagccc cagcgagaca
     6241 ccgtgcggga ccacggtcac cccgcgctcg cgcagccgca gcagggagtc gggcagatgc
     6301 ccggggcaca cgttctcggc gacggcctcg acccagtcga tgcccggcat ccgctccacg
     6361 gtgtccgcga tctccggccg ccagccgatc cccgtcccca gtcgcctcat ggtccccgcc
     6421 tccttcacgc cccctgcgtg gtgacggggg tatggccccg ccaccgccgc ccgaaccgcc
     6481 cgccgccctc cttcagagca acatttgagg ttcgcgccgc cccgcccgcc tggtccttgg
     6541 gaccgggggc taccggttcc tgagggccgg gtggtccgcg accaccgtgc agctgcccgg
     6601 ggcgatctcg gtgaagcccg cgtcccggac caggggcagt ccgctcgccg tcagctcgcc
     6661 ccagtgccgg ggcccggccg tgcgcacggc gagcgggaag cccgcctcgc gccaggcggt
     6721 ccgctcctcg tcggacagct cccaccaggc gagctgcgcg ccgtgcccgg cctgcgccat
     6781 cgccttgccc gcggacatcg tgacctcggg gttctgccag atcaccgggg ccgtcaggtc
     6841 gacgccgggc ggcggctccg gatcgtccag atcggttccg gagacctgga gcttcgccag
     6901 ctccctgggc cagccgtcga gcgggacggg cgggaagacc cgcacctgcg ccgccttgcc
     6961 ggtgaccgtg atgccgggca gggcctccgc ccgcagccac tcggcgcccc gcgcccgccg
     7021 caccaccttg cggatgcggg cgtcctgcca gtcccgcacg gccgcagccc actcgccgtc
     7081 gcccagcgcc cgctcgtcgg cgagcagggt cagcaccgcg cgagcggccg tctccagggc
     7141 gtccgtgcgg gccggcgggg cggcgcgctc gatacggacc accaggggca ggacgaactg
     7201 cggagcctcg tcccgggtcg acggctcgga acggaaaggg ctgtcgctgt cgccgggaac
     7261 ggccggatca ctggtcacgt cctccagtct gccaggtgga agatccatgg atccgcgagc
     7321 gggaggacgg tccgcatgcc aggtgagcta aggctggagg gggtgggccg ccgctacggc
     7381 gtgcgcggcc cgtgggtgct ccgcgacgtg catctgcacc cggcccccgg caccctgacc
     7441 cgcgtcgggg gccccaacgg cacgggcaag tccaccctgc tgcggctgct cgcccgcctc
     7501 gacgccccca ccgagggcag ggtcaccggc acgccccgga ccgcgtacgt ccccgaacgc
     7561 ttcccggcgg ccctcccctt caccgccctg cgctacctca cgcacctcgg cgccgtccac
     7621 ggcctgtccc gtacggcggc cgagcgggcg gcgggctact ggctggaccg cttcggcacc
     7681 ggcggccacg cccacacccc gatgagccgg ctctccaagg gcggcagcca gaaggtcgcc
     7741 gtggcgcagg cgctcctcgc cgaaccggag ctgctggtcc tggacgaggc ctggaccggg
     7801 ctcgacaccg acgcgcgcgc cgagctggac cgggcggtcg ccgagcggac cgccgccggg
     7861 tcggccgtcg tcttcgtcga ccacgatccc cgtcgcctcg ccggcctcgc ggacgccgag
     7921 tacacggtcg gcgacggcac cctgcgccgc cgtaccgcgc cggaaccggc ggccgccccc
     7981 gtcggaccgc acctgaccgt cgaggtgcga ggtccggccg gtgagctccc cgcgcaggcg
     8041 ctccggctgg cggcctccgc cgaggagacg gccgacggca cccaccggct caccgtcccc
     8101 gtctcccact cggacgtgct gctgcgcgtc ctgctcaccg cccgcccgcc gtggcacgtg
     8161 ctgagcgtca gcccgcgagg gggcgcccga tgaccgccct gctgcgctat cagagcgcgt
     8221 tgctcgtgcg ttcgcagcgc tggctgcccc cgttcctgct gtacgccgtg ttcctggccg
     8281 tcggggtgca ggccggcgag ccgctgctcg actcgctcgg ctacacggcc gcgttcctgc
     8341 tgcccgtggc cgcctggctg gtgcggatct gtgccggcgg tgaaccgcct gccgcccgca
     8401 gcgtcgtcgc ggccgcggcc ggtcccgcgc gggcgcacct ggcctgcgtc ctggtggcga
     8461 cgggggcggc cgccgcgttc ggcacggcgg cgaccgtggt cgtcacgctc gtcagcgatc
     8521 cggtcggcag cgaccaccgc acacgggtcc ccgtcctcgg ggccgcgggc gccgggctgc
     8581 tcgccgtcct gacctgcgcc ctgctcgggg cggccgtcgg cgcgctcacc gcctggcccg
     8641 tgctgcgctc accgggccgg ggggtgcccg cgctgctgct ggcggtcctg ctggcgctgg
     8701 tggggaccgg ctccccggca caggcggcgg tgagcggcct cgtcggcggc tcccgttcgg
     8761 cgaccgtccg tccgccggtg ctgccgctcg tggcggcggc cctggtgtgc gccgcggcga
     8821 ccgctctggc ctgcgtgctc acctcgcgcc gcgcaccgtg acgagttgtg cgtcgtcgcg
     8881 ccgggccacc cggcggatca ccccgagcag gcggtccgct gggcctcctg ccactcgcac
     8941 accgggcaca tcgtgatccc cttgtaggac tcgggatact cggtgggcct gcggcacagc
     9001 acgcactcgg cgtacggcgg cccctccgcc cggggcggcc gggtcgcgtc gatcaggcag
     9061 tagtcgcggt caccgtcgtc gtcgctcata cccccaccgt acgcagccgt ggacgacgcg
     9121 ggccgctcac cgccgtccgc ccgccgtcgc gatcagctcg gacaccttca cgaagcggta
     9181 gccgcgccgc cgcagctccg gcacgacggt ccgcaccacc tgctcggtcg tcggggccgc
     9241 gctgcgcgtg cagtgcatga cgacgacgga accgggccgg acgccgtcca gcacctgtct
     9301 cgccaccgcg tcggcgtcgg tcgcgaaggc gtccccgctc accacgtccc actgcaccgc
     9361 ggtgacgccg agaccgctca ccgcgcgcag cgcccgctgg tcgtaacagc cgccggggaa
     9421 gcggaagtac ggcatcgcgg agggcacccc ggccttgcgg aacgccgtgt acgcccgctc
     9481 cacgtccgtg cgcatcgcgt cctcggggac ggtcggcagg ccgtagcagt cgtcggtgaa
     9541 ggcgtggtgg ctgtaggagt ggttggcgat ctcgaagccg gggtcgcggc cgagggagcg
     9601 ggcctggtcg gggtactcct cggcccagcg gccggtcatg aacacggtgg ccggcacctt
     9661 gagcgtccgc agcgcggtga tcagccccgg gttgtcgaag tgctcgccgg ccgccgcgcg
     9721 gggcccctgg tcggcggtca tgtcggcgtc gaaggtgagg gcgaccgtct tgcccgcggt
     9781 gcggggcccg ttgcggaaga ccggggtcaa cccggccggc ccgggggcca gtgtcggagt
     9841 gcgggagggc gcggccgggg tccgggaggg cgcggccggg gtccgggaag gggtgggcgc
     9901 ggcgttccgc gcgccggcgg cgggggccgt cgcggagtcc gtggtccgga cggcgcgggt
     9961 gtcctgagcg gtgccgcagg cggtgagggg ggcgagggcg gcggcgaggg cgcagagggc
    10021 ggccacgcga cgtacgggag tgatcatcgt acgaaggtag gggcagtgcg ccgttggttg
    10081 tatgtatata tgcccgtgcc tggggcggtg tcacccgccc gggctctcac cacccgggcg
    10141 ggcgcggcgc ccgggctacc gccgccaggg tcccgtgacc gcgaacgtcg tccccggggt
    10201 gtagcagttg acgtacatcg tgcggccgtc gggggagaag gtgacgcccg cgaactcgcc
    10261 ccactcgggg gcctcggggg tgccgatgtt ctggcggccc cgcgccatcg cgtacacctc
    10321 gccggccctg gtgaccccga agacgtgctg cgcgccgttg ccgtcctcgc agaccatcag
    10381 accgccgctg ggcgccaggc agatgttgtc cggggactcc ccggggagct ggacgtcggt
    10441 gtcggggccg aagacgacga ccagggtcag gcgccgccgc tgcgggtcgt accgccagat
    10501 ctggccgaag tggtcggcag ccgaaccgtc cgagccgcgg gcgaacgacg acacgaagta
    10561 cacgcaccgc ccgccccagt aacagccctc cagcttctgc gcgtgcgtga tgcccttcgg
    10621 gccgaagtcc tggagccgga ccggggtcgt gacggcgagc gggtccggta cgtccaccca
    10681 ggtgatgccg tcgaaggagg cgcccgggtc ctggatcgag gacaggtcgg gcaccccggg
    10741 cacccgcatc gcctggagac ggccgcccgc gcgcagcgaa ccgaccccgc ccagcggctt
    10801 ctcgggcagg aagcggtaga agaggccgaa cggctccagg aacgcgtcct cggtctcgta
    10861 gacgacaccg cggtgcgggt cgacggcgat cgcctcgtgc tggaagcggc ccatcgcggt
    10921 cagcgggacg gccccgcccc ggcgcgggtc ggacgggtcg acctcgaaga cgaagccgtg
    10981 gtccttggtg tagccgttgg tgccggcctt gtcctcggtc tcctcgcagg tcagccaggt
    11041 gtgccaaggg gtgggcccgc cggcgcagtt gacggccgta ccggcgaggg cgacccgctc
    11101 ggagagcacg tcaccgcggg agtccagggt cagggccgta cagccgccct tgccggccgg
    11161 atcgtaggtc aggcccttca ccgtggggac ggagaacttc gccgtggggc ggttctcgtg
    11221 gttgcggacc aggtggacgc ggccgccctt gccgggcagg gccgtcatgc cgtcgtggtt
    11281 ggagggcacc ggaccctcac cggaccgcag cggatcgccc tcgcgggaga gcacccggta
    11341 gcggaacccc gcgggcaggt cgagcaggcc gtccgggtcc ggcacgagcg ggccgtagcc
    11401 ctcgtggccg
//
