LOCUS       NZ_BMSI01000002        11989 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence02, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000002
VERSION     NZ_BMSI01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 655051)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 351125
            Orig. end                         :: 363114
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11989
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11989
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11989
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6989](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6989)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(375..1883)
                     /locus_tag="IE189_RS07560"
                     /old_locus_tag="GCM10010496_15300"
     CDS             complement(375..1883)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126525.1"
                     /locus_tag="IE189_RS07560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15300"
                     /product="MFS transporter"
                     /protein_id="WP_189919070.1"
                     /transl_table=11
                     /translation="MSASATNEGPSTRESGSGQDPNRWRALWVTLVAGFMSLLDVTIVA
                     VALPTVQRELHASAAEVQWVVSGYALTFALALVTAGRLGDALDRRRIFLLALSCFVVFS
                     AACGAAPDITWLVVARLAQGLAAGFMAPQNSALIQQMFRGAERGRAFGFFGATVGISSA
                     VGPLTGGAILALASGSEGWRWIFYVNVPIGVVAVLLGRRLLPRTRPSGRAHVDVPGVVL
                     LGLGVLALMYPLVQADSGGLRRLWWLFPLAAALLAVFVWWQRRLVARDTPPLLDPRLFT
                     TVRGYAVGAGVGTLYFIGFSGVWLVFALFYQRGLGFSPLRSGLAVTPFALGSASAAVVA
                     GRLVDRFGRLLTVCGLTGVIAGLGGAVLLLRLLPLGTGPWAAAPLLFLGGIGGGFVVSP
                     NITMTLRDVPVRMAGAAGGALQTGQRLGAAVGTAALPGLYYLALGSGAGYRGALTVALA
                     AALAGMGASLVLAGADWRRDRRTVPAHERCPDEVAHDPLHSRQS"
     misc_feature    2351..2364
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.71"
                     /tool="antismash"
     misc_feature    2354..2367
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.58"
                     /tool="antismash"
     misc_feature    2367..2382
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.92"
                     /tool="antismash"
     misc_feature    complement(2368..2381)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            2433..4229
                     /locus_tag="IE189_RS07565"
                     /old_locus_tag="GCM10010496_15310"
     CDS             2433..4229
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657524.1"
                     /locus_tag="IE189_RS07565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15310"
                     /product="chitinase"
                     /protein_id="WP_189919072.1"
                     /transl_table=11
                     /translation="MNRSRPAVALLTAVALAVPAITALSSAAEAADAELARNGGFEAGL
                     DGWTCTAARSVTSPVRSGSSALEATPAGNDHAQCSQTVTVRPDSQYTLSGYVRGAYVYL
                     GASGTGTTDVSTWTQSAPDWQRLGTTFRTGASTTRVTIYTHGWYGTGAYQADDISLLGP
                     GGSTGVPPATPTGLKAGAPSATSVALSWSAASGATSYALYRDGVKTQTVNGTSAVVTGL
                     SASMAYTFQVAGVNDAGESAKSAAVTVTTAASGGGGPSDLPAHALVGYLHASFANGAGY
                     TRLADVPDSWDVIDLAFGEPTTTTSGDIRFTRCPVTECPNVESDADFKAAIKAKQAAGK
                     KVLISIGGQNGQVQLTTAAARDTFVSSVSKIIDTYGLDGLDVDFEGHSLSLNADDTDFR
                     NPRTPVIVNLISALRTLKARYGSGFVLTMAPETFFVQMGHQFYGTGKWGGQDPRCGAYL
                     PVIHALRDDLTLLHVQDYNSGPIMGLDNQYHSMGGADFHTAMTDMLLTGFPVAGDAGAV
                     FPPLRPDQVAIGMPASTNAGNGYVPPAEVVKTLSCLTERTGCGSYAPHGTWPALRGLMT
                     WSINWDRFAGGEFQRTFDGYFG"
     gene            complement(4285..4944)
                     /locus_tag="IE189_RS07570"
                     /old_locus_tag="GCM10010496_15320"
     CDS             complement(4285..4944)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129142.1"
                     /locus_tag="IE189_RS07570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15320"
                     /product="DUF5825 family protein"
                     /protein_id="WP_189919073.1"
                     /transl_table=11
                     /translation="MTTIAPHFTIRAWRDLDPQARGLPGMSLGTLPVTAGAPQADRLWD
                     LGVRHAELDTAVDLATSDPAAHRGVVDRLCLVRDLTARAVSVDWDLRLSPQDAAHQWKV
                     LSHLQPPRSLTGLENGEEALHAWRTRHYLCKLVWRQGPGFIQIRDRRWGDLRRFTADEP
                     GYREAIARLDQGAGRADVPADVLAEFTAEHLVLSVGELAWWLPYRVSRWLQEAMAV"
     misc_feature    4930..4945
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.73"
                     /tool="antismash"
     gene            complement(5001..6989)
                     /locus_tag="IE189_RS07575"
                     /old_locus_tag="GCM10010496_15330"
     CDS             complement(5001..6989)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088743.1"
                     /locus_tag="IE189_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15330"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_189919074.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.5e-188, bitscore:
                     622.4, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 4.1e-12, bitscore: 42.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDEHVQDATCEVLHANLEF
                     TDWITSRTEFTAEDYEYYALSSYFLGCGDWVFSSALYDDPEWREPEFVEVMSSKLRRAR
                     MRMTRELHRVVPEFVAEVARKVVELRPDVVGFTSTFQQNTAALAAARQIKRLAPHVVTV
                     MGGANCDAEQGAAVHRNFPFVDYVVRGEGEDAFPRLLTALRDGDPAAAASIPGLCHRAA
                     GSAHTANPMPSGPLPPAAILPPDYSGYFERLAASVARGWVEPKLVVEGARGCWWGEKHH
                     CTFCGLNGSFMEFRSKSPDTFYREIMELAERHQVLDMYVVDNILDMRYLSTVLPRIVDS
                     GYDLRMHIEIKANMRRPQLQVLADAGLIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLR
                     DAAETGLSVSWNYLHGFPGETAEDYEPVIRQLPALEHLNPPVDLSARIAIERFSPYFKR
                     PELGFTGLRPEEHYLFTYALPESELHDLAYVFQAPERGIGEPTVTLLNDGIAAWKKHHA
                     DARLTHTDLGERIVLVSRRASFAWRTMQLTDPLHTALFRLLDQPHTVVALHRKLTSADD
                     TPDGAGPAPHDLEALLEEWVARGIVFTDAGQYVHIAPAAVNQDLLRLDYMRHLHRRTAS
                     DDGSGPEADARAGAAVGVEAGAVPAPS"
     gene            complement(7095..7217)
                     /locus_tag="IE189_RS39500"
                     /old_locus_tag="GCM10010496_15340"
     CDS             complement(7095..7217)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993374.1"
                     /locus_tag="IE189_RS39500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15340"
                     /product="hypothetical protein"
                     /protein_id="WP_267928680.1"
                     /transl_table=11
                     /translation="MQKDIIQNDPLAGDEENRKPGIGITITIPFRNAAESDDEE"
     gene            complement(7295..9106)
                     /locus_tag="IE189_RS07580"
                     /old_locus_tag="GCM10010496_15350"
     CDS             complement(7295..9106)
                     /EC_number="3.4.-.-"
                     /NRPS_PKS="Domain: Abhydrolase_1 (358-499). E-value:
                     3.8e-10. Score: 31.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS07580_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357534.1"
                     /locus_tag="IE189_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15350"
                     /product="S9 family peptidase"
                     /protein_id="WP_229901121.1"
                     /sec_met_domain="PF00561 (E-value: 7.4e-08, bitscore: 27.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 7.7e-40, bitscore:
                     131.8, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADTFKVFLSALPHVCPGDPRRMVLTADADGRCEVFTWNAATRTT
                     RQVTDRPQGTVHCAVDADAYVWWFDEDPHGRGHWWFQPFDGGCRLPGLPGAPAGLQRGL
                     AVTACGTVAAALAPPHGGTRLLVGRRGRVPREILRVPGEGRLAGITPEGDLVALATAAG
                     ADAAVTVVTSTGRVAARLPGNDDPAGRLWCRDFSPRPGRRELLLVHELEDGYRLATWTP
                     RRGLVTHAWCAFDTEITAGWYPDGDDVLVRQERHGRSLLHRVSLRARTRTVVPTPPGTL
                     LDAAPRAGGALHYLWTDTAHPPVPRTTDGTALPTPGLLPRVPGRHTELWTPTPHGPVHT
                     LLSLPDPADDGPRGAAAPPTVFAVHGGPADHDRDAYDATVHSLVASGYAVARVNYRGST
                     GYGPRWRRALEAGVGHPQVDDLAAVRADLVRRGLADARATALWGVSWGAYLVLLALGTR
                     PDLWQAGVAVKPVADWVTAHRTTTPALRALDVRWFGGTPDEVPERYARSSPLTFAADVR
                     APLLVVGADKDAKCPPEQIRSYLDALTRAGVPHERMWLDTGHDGYDGAAHVAVLRRALT
                     FLRTHLPAPAPGAPSRPPARPGGPRSR"
     aSDomain        complement(7610..8032)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS07580_Abhydrolase_1.1"
                     /evalue="3.80E-10"
                     /label="IE189_RS07580_Abhydrolase_1.1"
                     /locus_tag="IE189_RS07580"
                     /protein_end="499"
                     /protein_start="358"
                     /score="31.7"
                     /tool="antismash"
                     /translation="PTVFAVHGGPADHDRDAYDATVHSLVASGYAVARVNYRGSTGYGP
                     RWRRALEAGVGHPQVDDLAAVRADLVRRGLADARATALWGVSWGAYLVLLALGTRPDLW
                     QAGVAVKPVADWVTAHRTTTPALRALDVRWFGGTPDE"
     gene            complement(9099..10511)
                     /locus_tag="IE189_RS07585"
                     /old_locus_tag="GCM10010496_15360"
     CDS             complement(9099..10511)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569708.1"
                     /locus_tag="IE189_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_15360"
                     /product="MFS transporter"
                     /protein_id="WP_229901122.1"
                     /transl_table=11
                     /translation="MLVLGGVGNVDASPIRRPPGGPDPAHDEHSPTAGRPQDTERPRHT
                     KRPDPAAGPSGRADRDLRILWWVNAVDGLGSQASGLVLPLLLLELGHSPGTAGSLAGAA
                     ALTGVVLGPLVAIPADRGRRRRIMTGSAALAALATGVLALTCLGRPALWLVVSLALAER
                     LFAVTYEAASRGALVHLAAADELPRATAGMQVGDQVALIAGPALGGALFQLARPVPFLA
                     DALSYTAAAVGIRAIRTPLDTPAPPPDAPPDKASEGRGRWARRFDAARAGVVTVAASAV
                     LRLVLVWTSAAGGALTLLFYTALFRLGGDGRGAATGLVLAAAGAAGLAGSLVAAPLVRR
                     LGARRLLTTAAWLLPLPCGSLLWADGATAWGVAFAGLSLLVPLITVVLSSAAVACTPAA
                     LQSRTASVLGSVSALAAAGAPALAALLVTAWNLRTPALLCTVLFVVLAAYTQFKAPAAL
                     RAAAAGRVDGRG"
     misc_feature    complement(10056..10058)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    10545..10560
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.96"
                     /tool="antismash"
     misc_feature    complement(11092..11105)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.77"
                     /tool="antismash"
     misc_feature    complement(11506..11521)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.16"
                     /tool="antismash"
     misc_feature    complement(11587..11602)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
ORIGIN
        1 ggatacgcgg ccgatctcca ggccgccgac cccggctcga tgctggtcct ctaccgcacc
       61 gcgctgcggc tgcgacgcgg cgaggccgcc ctcggtgacg gcgtgctgga atggctgccc
      121 gcccccgccg gcgtcctcgc gttccggcgg acacccggcc tgatctgcgt ggtcaacctg
      181 gcggccgaac cggccgtgct gccggaacac gatcagctcc tgctcgccag cggacccctg
      241 gactccgcgg gccggctgcc ccgcgacacg gcggtgtggc tgcgcgagtg accggtccgc
      301 acctcaccgc acggcctgct ccccaccgat cgagggccct cgtccgccgc gcccggcgcg
      361 gagggtccgc ccgatcacga ctggcgggag tgcaacgggt catgggcgac ctcgtcggga
      421 cagcgctcgt gggccgggac cgtccgccgg tcgcgccgcc agtcggcgcc ggcgaggacc
      481 agcgacgcgc ccatgcccgc cagggccgcc gccagcgcga cggtcagggc gccgcggtag
      541 ccggcgcccg acccgagcgc caggtagtac aggccgggca gcgccgccgt tccgacggcg
      601 gcgccgagac gctgtccggt ctggagcgcg ccgccggccg cgccggccat ccgcacgggt
      661 acgtcccgca gggtcatggt gatgttcggc gagaccacga agccgccgcc gatcccgccg
      721 aggaagagca gcggagcggc ggcccagggg ccggtaccga ggggcaggag acgaaggagc
      781 agcacggctc cgcccaggcc ggcgatcaca ccggtgagac cgcacaccgt gagcagccgt
      841 ccgaaccggt cgaccagcct gcccgcgacg acggccgcgc tcgccgatcc cagggcgaag
      901 ggcgtcacgg ccaggccgga gcgcagcggc gagaagccga gcccgcgctg gtagaagagg
      961 gcgaagacca gccagacccc gctgaacccg atgaagtaca gggtgccgac cccggccccg
     1021 acggcgtagc cgcgcacggt ggtgaacaga cggggatcga gcagcggcgg ggtgtcacgg
     1081 gcgaccagac ggcgctgcca ccacacgaag acggcgagca gcgcggcggc gagcgggaag
     1141 agccaccaca ggcggcgcag cccgccggag tccgcctgga ccagcggata catgagcgcg
     1201 aggaccccga ggccgagcag cacgacgccg ggcacgtcca cgtgggcccg gccggacggc
     1261 cgggtgcggg gcagcaggcg acggccgagc agcacggcca ccacgccgat cgggacgttg
     1321 acgtagaaga tccagcgcca gccctcggag ccggaggcca gcgcgaggat ggcgccgccg
     1381 gtgagcgggc cgacggcgga ggagatgccg accgtggcgc cgaagaaacc gaaggcccgt
     1441 ccgcgctcgg cgccgcggaa catctgctgg atgagcgcgg agttctgcgg ggccatgaaa
     1501 ccggccgcca gcccctgggc gagccgggcc accaccagcc aggtgatgtc gggggcggcg
     1561 ccgcaggccg cgctgaagac gacgaagcag ctcagggcca gcaggaagat ccggcgccgg
     1621 tccagcgcgt caccgagccg gccggcggtc accagggcca gggcgaaggt gagcgcgtag
     1681 ccggagacca cccattgcac ctctgccgcg gaggcgtgca gctcacgctg gacggtgggc
     1741 agggcgaccg ccacgatcgt cacgtccagc aggctcatga aaccggccac cagagtgacc
     1801 cacagggccc gccaccggtt cggatcctgc ccggatccgc tctcccgcgt ggacgggccc
     1861 tcgttcgtcg ccgacgccga cacaaggcct cctctcgccg ggccgcctcc gagaagcacg
     1921 tgatcacgcg gtggacgcgg gcccgctgtg ccccagtacc caccctgccg ccgtccggca
     1981 caccgatcgc ccgcagtctc cctgagcgca ttctcgacgg catcaccccc gcaccgcatg
     2041 ccgaggggcc gccggcgccg cgccgggggt caggccgaaa ctccgatgcc ccgttcgcgg
     2101 tggctcggta gggtcggggc cgtgcaggtg cgcaggcacc ggacacttcg cctcttcagc
     2161 gggaaacgcg ggttcgaatc ccgtcgccgg ctccggccgg tgtcgtccag cggcccagga
     2221 cacctatggt ccgtcgccga cctcgatctc tgcacacctc agtgaacccg gcagggccac
     2281 cgccccgccg ggttccgtgc gtcgcgcccg cacggcgctc gctcctgctt cccctccggg
     2341 cctcctctcg aggtcttgac aaaagaattg gtctggacca agttgggcgc gccccgcccc
     2401 gcagtccgaa cccccactgt ccggaggcac ttgtgaaccg ctccagaccg gccgtcgccc
     2461 tgctcacggc ggtggccctc gccgtacccg caatcaccgc actgtcgtcg gccgcagagg
     2521 ccgccgacgc cgaactcgcc cgcaacggcg gcttcgaggc gggactcgac ggctggacgt
     2581 gcaccgcggc caggtccgtc accagcccgg tgcgcagcgg cagttcggcc ctcgaggcga
     2641 cccccgccgg caacgaccac gcccagtgct cccagacggt caccgtgcgg cccgactccc
     2701 agtacacgct gtccggttac gtccgtgggg cgtacgtgta cctcggcgcg agcggcacgg
     2761 gcaccaccga cgtctccacg tggacccagt ccgcccccga ctggcagcgg ctcggcacca
     2821 ccttccgcac gggcgcgtcc acgaccaggg tgacgatcta cacgcacggc tggtacggca
     2881 ccggcgcgta ccaggccgac gacatctcgc tgctcgggcc gggcggctcc accggtgtac
     2941 cgcccgccac gccgaccggc ctgaaggccg gggccccctc ggccacgagc gtcgccctct
     3001 cctggtcggc ggcgagcggc gcgacgagct acgcgctcta ccgtgacggc gtgaaaacac
     3061 agacggtgaa cggtacttcg gccgtcgtga ccggtctgtc cgcctcgatg gcctacacct
     3121 tccaggtcgc cggcgtgaac gacgccggag agtccgccaa gtcggccgcc gtgacggtga
     3181 cgaccgccgc gtccggcggc ggaggcccct ccgacctgcc cgcccacgcc ctggtcggct
     3241 acctgcacgc gagcttcgcg aacggtgccg gatacacccg actggccgat gtccccgaca
     3301 gctgggacgt catcgacctg gccttcggcg aacccacgac caccacctcc ggcgacatcc
     3361 gcttcacgcg ctgccccgtg accgaatgcc cgaacgtcga gagcgacgcc gacttcaagg
     3421 cggcgatcaa ggccaagcag gcggccggca agaaggtgct gatctcgatc ggcgggcaga
     3481 acggtcaggt ccagctcacc acggcggcgg cccgcgacac cttcgtctcc tccgtctcca
     3541 agatcatcga cacgtacggc ctggacggcc tggacgtcga cttcgaaggc cactccctgt
     3601 cgctgaacgc cgacgacacg gacttcagga accccaggac acccgtgatc gtgaacctca
     3661 tctcggcgct caggaccctc aaggccaggt acggctccgg gttcgtgctc accatggcgc
     3721 ccgagacgtt cttcgtgcag atgggccacc agttctacgg cacggggaag tggggcgggc
     3781 aggacccccg gtgcggcgcg tacctgccgg tgatccacgc cctgcgcgac gacctgaccc
     3841 tcctgcacgt ccaggactac aactcgggcc cgatcatggg cctcgacaac cagtaccact
     3901 ccatgggcgg cgccgacttc cacaccgcca tgaccgacat gctcctgacc ggcttcccgg
     3961 tggccggcga cgccggcgcg gtcttcccgc cgctgcgccc cgaccaggtc gccatcggca
     4021 tgccggcctc caccaacgcg ggaaacggct acgtcccccc ggccgaggtg gtcaagacgc
     4081 tgtcgtgcct gaccgagcgg accggctgcg gctcgtacgc cccccacggc acctggccgg
     4141 ccctgcgcgg cctcatgacg tggtcgatca actgggaccg cttcgccggc ggggagttcc
     4201 agcggacgtt cgacggctac ttcggatgac ccggagccac gagaacccgg tgcgcccacc
     4261 ggaccggtgg gcgcaccggg gtgctcagac ggccatggcc tcctggagcc agcgggacac
     4321 ccggtagggc agccaccacg ccagttcgcc gacgctcagg accaggtgct cggcggtgaa
     4381 ctccgccagc acgtccgcgg gcacgtcggc ccggccggca ccctggtcga gccgcgcgat
     4441 ggcctcgcgg tacccgggtt cgtccgccgt gaagcggcgc aggtcccccc agcggcggtc
     4501 gcggatctgg atgaagcccg gcccctggcg ccagaccagc ttgcacaggt agtgccgggt
     4561 ccgccaggcg tgcagcgcct cctcgccgtt ctcgaggccg gtgagactgc ggggcggctg
     4621 gaggtgggac agcaccttcc actggtgggc cgcgtcctgt ggtgacagcc gcaggtccca
     4681 gtcgacggag acggcccgcg cggtgaggtc gcggaccagg cacaaccggt cgacgacccc
     4741 gcggtgggcc gcgggatcgc tcgtggcgag gtcgacggcg gtgtccagtt cggcgtggcg
     4801 cacgcccagg tcccacagcc ggtcggcctg cggggccccg gccgtcacag ggagcgtccc
     4861 cagcgacatg cccggcagcc cgcgggcctg cgggtccaga tcacgccagg ccctgatcgt
     4921 gaagtgcggt gcgatggtgg tcacggtgcg gtccttcgtg ggtcgggcag ccggtggccg
     4981 cgggatgagg ggaccggcgt tcaggacggc gcgggcacgg cgccggcctc gacgccgacg
     5041 gcggccccgg cccgggcgtc ggcctcgggc ccgctcccgt catcggacgc cgtacggcgg
     5101 tggagatggc gcatgtagtc caggcgcagc aggtcctggt tcacggcggc gggggcgatg
     5161 tgcacgtact ggcccgcgtc ggtgaagacg atgccccgcg cgacccactc ctccaggagg
     5221 gcctccagat cgtgcggtgc ggggcccgcg ccgtccgggg tgtcgtcggc cgacgtcagt
     5281 ttgcggtgga gcgccaccac ggtgtgcggc tggtccagca gccggaagag cgccgtgtgc
     5341 aacggatcgg tgagctgcat ggtccgccag gcgaacgagg cccgcctgct gaccagcacg
     5401 atccgttcac cgagatcggt gtgggtgaga cgggcgtcgg cgtggtgctt cttccaggcg
     5461 gcgatgccgt cgttgaggag ggtgacggtc ggctcgccga tgccgcgctc cggggcctgg
     5521 aagacatagg ccaggtcgtg cagttcggac tcgggcagcg cgtaggtgaa caggtagtgc
     5581 tcctcggggc gcagtcccgt gaagcccagt tcggggcgct tgaagtaggg actgaaccgt
     5641 tcgatggcga tgcgtgcgga caggtcgacc ggcgggttga ggtgttccag ggcgggcagc
     5701 tggcggatga cgggttcgta gtcctccgcg gtctccccgg ggaatccgtg caggtagttc
     5761 caggacaccg acaggccggt ctccgccgcg tcgcggagca tgcggacgtt ctggcagccg
     5821 ctgaccccct tgtccatgag gtcgaggacg cggctgttga ggctctcgat gcccggctgg
     5881 acgtagatga gcccggcgtc cgccaggacc tggagctgag ggcggcgcat gttcgccttg
     5941 atctcgatgt gcatgcgcag gtcgtagccg ctgtcgacga tgcggggcag cacggtggac
     6001 aggtagcgca tgtccaggat gttgtcgacc acgtacatgt cgagcacctg gtggcgttcc
     6061 gccagctcca tgatctcccg gtagaacgtg tcggggctct tgctgcggaa ctccatgaag
     6121 gagccgttca gtccgcagaa cgtgcagtgg tgcttctctc cccaccagca gccgcgtgcg
     6181 ccctcgacca ccagtttcgg ctccacccag ccgcgcgcca cggacgcggc gagccgctcg
     6241 aagtagccgc tgtagtcggg aggcaggatc gccgccggcg gcagcggccc gctcggcatc
     6301 gggttggcgg tgtgcgccga cccggccgcg cggtggcaca gccccgggat cgaggcggcg
     6361 gccgccgggt ccccgtcgcg cagcgcggtc agcagccggg gaaaggcgtc ctcgccctcg
     6421 ccgcgcacca cgtagtccac gaaggggaag ttgcggtgga ccgccgcgcc ctgctccgcg
     6481 tcgcagttgg ccccgcccat gacggtcacg acgtgcggtg ccaggcgctt gatctggcgg
     6541 gccgcggcca gcgcggcggt gttctgctgg aaggtcgagg tgaacccgac cacgtcgggc
     6601 cgcagttcca ccaccttgcg ggccacttcg gcgacgaact ccggtaccac acggtgcagt
     6661 tcgcgcgtca tgcgcatccg ggcgcgccgc agtttggacg acatcacctc gacgaactcc
     6721 ggttccctcc actcggggtc gtcgtagagc gcggaggaga acacccagtc gccgcagccg
     6781 aggaagtagg acgacagggc gtagtactcg tagtcctcgg cggtgaactc ggtgcggctg
     6841 gtgatccagt cggtgaactc cagattggcg tgcagcacct cgcacgtggc gtcctggacg
     6901 tgttcgtcga cgctgcgctt gaggatgccg agcgccaggg agggcaggtc tatcggggac
     6961 cagggcatgt tgacgagaag aacgcgcacg gtgggctcct tgcgcacggc ggcctgcgga
     7021 cgggaagtgc ggtacgggaa gtgcgggagg gagcccggcc ggcccgtgtc gagtcgggcc
     7081 ggccgggccg tgggtcactc ctcgtcgtcg ctctcggcgg cgttgcggaa cgggatcgtg
     7141 atggtgatgc cgatgcccgg cttcctgttc tcctcgtcgc cggcgagcgg atcgttctgg
     7201 atgatgtcct tctgcacggt gttcctcctt ctcggattca tgacgtgttc ttcgggcggg
     7261 tggtgctgtg cgcttcccgg tcccgctgag tcggtcagcg ggaccggggt cctcccggac
     7321 gtgccggggg acgcgagggg gcgccgggcg ccggggcggg cagatgcgta cgcaggaagg
     7381 tcagggcgcg gcgcagcacg gcgacgtggg ccgcgccgtc gtagccgtcg tggccggtgt
     7441 ccagccacat ccgctcgtgg ggcacgcccg cgcgggtcag ggcgtccagg tagctgcgga
     7501 tctgctcggg cgggcacttc gcgtccttgt cggcgccgac cacgagcagc ggtgctcgca
     7561 cgtcggccgc gaaggtcagg ggcgaactac gggcgtaacg ttccggcacc tcgtcgggag
     7621 tgcctccgaa ccagcggacg tccaaggcgc gcagtgcggg cgtggtcgtg cggtgggcgg
     7681 tcacccagtc ggcgacgggt ttcacggcga ccccggcctg ccacaggtcg ggccgggtcc
     7741 cgagggcgag caggaccagg tacgcgcccc acgagacccc ccacagggcc gtcgcgcggg
     7801 cgtcggcgag tccgcggcgc acgaggtcgg cccggacggc ggcgaggtcg tccacctggg
     7861 ggtggccgac cccggcctcc agggcccgcc gccagcgggg gccgtagccc gtcgagccgc
     7921 ggtagttgac gcgtgcgacg gcatagccgg aggcgacgag cgagtgcacc gtggcgtcgt
     7981 aggcgtcccg gtcgtggtcg gccgggccgc cgtggacggc gaacacggtc ggcggcgcgg
     8041 cggcccctcg gggcccgtcg tcggcggggt ccggcaggct cagcagggtg tggaccggtc
     8101 cgtggggcgt cggggtccac agctccgtgt gccgtccggg gacacgcggc aggagtccgg
     8161 gcgtcggcag ggcggttccg tcggtggtgc ggggcaccgg tggatgcgcg gtgtccgtcc
     8221 acaggtagtg cagtgccccg cccgcacgcg gggccgcgtc gagcagggtc cccgggggag
     8281 tggggacgac ggtccgggta cgggcgcgca gggacacccg gtgcagcagg gaacggccgt
     8341 ggcgttcctg gcgtaccagg acgtcgtcgc cgtcggggta ccagcccgcg gtgatctccg
     8401 tgtcgaaggc gcaccacgcg tgggtgacga ggccgcgccg cggggtccac gtggccagcc
     8461 ggtagccgtc ctccaactcg tgcaccagca gcagttcccg ccgtccgggc cgtggcgaga
     8521 agtcccggca ccagagccgg cccgccgggt cgtcgttccc gggcagccgg gcggcgaccc
     8581 ggcccgtgga cgtgacgacg gtgacggccg cgtccgcgcc ggccgcggtc gcgagggcca
     8641 ccaggtcacc ctccggggtg atgccggcca gccggccctc gccggggacg cgcaggatct
     8701 cgcgcggtac ccggccgcgt cgtccgacga gcagccgcgt gccgccgtgc gggggtgcca
     8761 gtgccgcggc gaccgtgccg caggcggtga ccgccaggcc gcgttgcagc ccggcggggg
     8821 cgcccggcag gccgggcagc cggcagccgc cgtcgaacgg ctggaaccac cagtgcccgc
     8881 ggccgtgcgg gtcctcgtcg aaccaccaca cgtacgcgtc ggcgtcgacg gcgcagtgca
     8941 ccgtgccctg tggccggtcg gtgacctgcc gggtggtccg ggtggccgcg ttccacgtga
     9001 acacctcgca gcggccgtcg gcgtcggccg tcagcaccat gcgacgcggg tcgcccgggc
     9061 agacgtgcgg cagcgcgctg aggaagacct tgaacgtgtc agccacggcc gtccaccctt
     9121 ccggcggcgg ccgcgcgcag ggccgcgggc gccttgaact gtgtgtacgc ggccagcacg
     9181 acgaacagca cggtgcacag cagcgcgggc gtgcgcaggt tccaggcggt gaccaggagg
     9241 gccgcgagcg ccggagcgcc cgccgcggcc agcgccgaga ccgagccgag cacgctcgcg
     9301 gtacgcgact ggagtgccgc cggtgtgcac gcgacggcgg cactggacag caccacggtg
     9361 atcagcggca ccagcaggga cagcccggcg aacgccactc cccaggccgt cgcaccgtcc
     9421 gcccacagca gggagccgca cggcagcggc agcagccacg cggccgtggt cagcaggcgg
     9481 cgggcgccca accgccgtac caggggcgcc gcgaccaatg agcccgccag ccccgccgct
     9541 cccgccgcgg ccaggaccag gcccgtcgcg gcgccacgcc cgtcgccgcc gagccggaac
     9601 agggccgtgt agaacagcag ggtcagggcg ccgccggccg ccgacgtcca gaccaggacg
     9661 agacgcagca ccgcagacgc ggccaccgtg accacgcccg ctcgcgcggc gtcgaaccgc
     9721 cgggcccacc tcccgcgtcc ttcggaggcc ttgtccggcg gtgcgtccgg gggcggggcg
     9781 ggggtgtcga gcggggtgcg gatggcccgt attcccaccg cggcggcggt gtaggagagc
     9841 gcgtcggcga ggaacggcac cggccgggcg agttggaaca gggctccgcc gagcgcgggg
     9901 cccgcgatca gcgcgacctg gtcaccgacc tgcatccccg cggtcgcccg gggcagttcg
     9961 tcggcggcgg ccaggtggac gagggcgccg cgcgacgcgg cctcgtaggt caccgcgaac
    10021 agccgttccg ccagggcgag ggacaccacg agccataacg ccgggcggcc caggcaggtc
    10081 agcgccaaca cccctgtggc cagggccgcc agggcggccg agccggtcat gatgcgccgc
    10141 cgccgtcccc ggtcggccgg tatcgcgacg agcgggccga ggacgacccc ggtgagcgcg
    10201 gcggcgccgg ccagcgatcc ggcggtgccg gggctgtggc ccagttccag gagcagcagc
    10261 gggaggacca gcccggaggc ctgactgccg agcccgtcga ccgcgttgac ccaccacagg
    10321 atcctgaggt cccggtccgc ccgcccggac gggccggccg ccgggtccgg ccgcttcgtg
    10381 tgccgcggcc gctcggtgtc ctgcggccgt ccggcggtcg gggagtgctc gtcgtgcgct
    10441 ggatcgggcc cgccgggagg ccggcggatc ggtgacgcgt cgacgttccc cacccctccc
    10501 agtacgagca cgggaggcca cggcgcgggg ccgtctctcc tgtgtcgaaa gtgtgtgagc
    10561 cgtgaaagtg agcgggagcc taaccatggc ggaacgtcag cgacaagtgc cgttcagtgc
    10621 gcaatcgtca cctgatacgc ggacatatgg ccgacggccg actggtcacg aaacgtcggc
    10681 ccgtgcagcc ggaaggtgcc ggtcggcgga cgcggcccac cgccggcggg agtggttccg
    10741 cttcgcatcc gcatatcggc ggcgttgcat tccgggctcg ccgacggggc gtgcgaaccg
    10801 tgtccacgac accgcacgcg ttcccgtgtg ccgtgctccc gcctgcggtc cggcccgtgt
    10861 tgccggcccc ggaaccggtt gcgctgccga acgcggactc cgccgggcgg cgcccacgac
    10921 gcggctcggg ggtctccgtc gcgtcgcggg taccgcgcgg ggaccctcgc gcgtccgtgg
    10981 cgtccgagcg gcgcattggc cgacaatccg ctctccccgc cctcgtcgcc gaggacgggc
    11041 gggggctccg cggggtgctt tggagcgaag cgtggcgatg tatcgattcg gcgaacgaat
    11101 gtgcgcaatg tgaaggcgca gtgcgaatgg cgtgattgaa tcgtgcacaa cttggtcagt
    11161 tggcttgatc tggtcgttcg gcaccccttg gcctgcccgt tcgccgggac cgcggggcgt
    11221 cgtaccgcct gcgcccggcg gcgcgcggac gcgtgcgccg gaccgcttcg gctgagccgt
    11281 cggccgtcac ccaccccgaa ggagcgtcga tgaccgccac cgtctccaac ttctactacg
    11341 gggccgcgac gccgatcgcg gcctacctga tggcctgtct cggcgcggcg ctcggactgc
    11401 gctgcacgac acgctccctg cgccgcccgc accgcagagc cagatggctg gccctgggcg
    11461 cggtgtccat cggctgcggc atctggacca tgcacttcat cgccatgatc ggcttcagcg
    11521 tccagggcgc actggtcgag tacgacgcga cgaagaccgt cctcagcctc gtcgtcgcca
    11581 tcgtcgtcgt cgccatcggc gtcttcctgg tgggctaccg gggcggctcg gcctccaacc
    11641 tcgccgtcgc gggcacgatc accggactcg gggtcgcggc catgcactac ctcggcatgg
    11701 ccgccatcca caccaacggc ggcctgcact acgacacgcc gaccgtggcg ctctccgtcg
    11761 ccatcgccgt agcggccgcc acggcggcgc tgtgggcggc ggtctccatc cgcggtctgt
    11821 ggtccagcct gggagcgagc ctggtcatgg gcgtggccgt gaccggtatg cactacaccg
    11881 gcatggccgc ggtctccgtg cacctcaccg gcggttccgc cgtctcgcag tcctccaccg
    11941 gtctgctgtc gttcctcctg ctgatgctgg ccggaccgct cgtggtcct
//
