LOCUS       NZ_BMSI01000001        10483 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence01, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000001
VERSION     NZ_BMSI01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1356280)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1002270
            Orig. end                         :: 1012753
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10483
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10483
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10483
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5483](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5483)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(65..80)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.75"
                     /tool="antismash"
     gene            345..2180
                     /locus_tag="IE189_RS04485"
                     /old_locus_tag="GCM10010496_09110"
     CDS             345..2180
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132945.1"
                     /locus_tag="IE189_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09110"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_189917994.1"
                     /transl_table=11
                     /translation="MLTEVAEAVRLQEGPPGVRSVLRALRRLEPASTKDLSRATGLPVP
                     IVAALGNELRRRGFVTAQRPSRLTEDGRDLVAELGMDLSLDATCRTCDGRELVIPDVLD
                     EAVRRLRALMESGPAVDMAIDQSHCTAETKVRRVLALLRAGALPGGSLLLIGDDDLVSL
                     AVAVVGDVLGGPIVERVTVVDISPEILDYIQKVSAGLGTRVETVRHDLRLPLPEALHQQ
                     HDVAMTDPPYTPEGARLFLSRAVEGLRPGPAHSIFFSFGGKSPDEMLEVQREIIGLGLI
                     TNGYIRNFNEYEGSGILGGVGFFQHLLTTTSTAPSQDGEFSGPLYTGDKRTRQREYTCV
                     ACDARLRVGPGARWASVGALREEGCPNCGKGPFRPGRLVPAEDPAPSAEQPATPVTAAG
                     GPADAGPPVTPTTPATPDAPVSASPGASAPAPSVGRAHGWRAPSEAERAALAERARPYV
                     TRGADERDLPAIGRFEAEIARVSFGEDAIDDPQRWASRLGRAMEKSKEGMIVADAPGGE
                     PVGWCWVSINQNAMTGDRYANFRSLAVSPVDNRGDVAELLLTAGLEFCLANGITEVVGR
                     VHVGNVPMRTVYRKFGFDPTSLSMKLFLPQGTDGR"
     gene            2177..3331
                     /locus_tag="IE189_RS04490"
                     /old_locus_tag="GCM10010496_09120"
     CDS             2177..3331
                     /NRPS_PKS="Domain: FkbH (12-133). E-value: 2.6e-16. Score:
                     51.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS04490_FkbH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1256: FkbH like protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132944.1"
                     /locus_tag="IE189_RS04490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09120"
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="WP_189917996.1"
                     /transl_table=11
                     /translation="MSVADGFDPDGVKCVVWDLDGTLWDEIAVESPTDDLPAPRPATLA
                     AIDDLAARGVLSSIASRSAPSVLDRLDAVPGLRARFLAPQVSWQDKSESLRRIAEELGI
                     AVEALVLVDDSPYERAEVEALLPGVRTLDPADVPALLAAFEGREITPESRARVGRYRTE
                     ETRRAEGARFRGSREEFLRWCDMRLTVGAATADELPRALELAARTHRLNSSGLTPDRLR
                     ELAAADGHELYTARMTDRFGDYGIIGAALVDRGPDTPGVRDGAGTASATWAVPLLALSC
                     RVAGRGAAAAFLFRLMERAREAGAEEFRVTLRPTDANLEMRILLRQAGLRRTDAAVPGP
                     ADASGAPVARTAPAEAAVLGRTLGGDVPGPPAWLHVSDREDTEA"
     aSDomain        2213..2575
                     /aSDomain="FkbH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS04490_FkbH.1"
                     /evalue="2.60E-16"
                     /label="IE189_RS04490_FkbH.1"
                     /locus_tag="IE189_RS04490"
                     /protein_end="133"
                     /protein_start="12"
                     /score="51.7"
                     /tool="antismash"
                     /translation="KCVVWDLDGTLWDEIAVESPTDDLPAPRPATLAAIDDLAARGVLS
                     SIASRSAPSVLDRLDAVPGLRARFLAPQVSWQDKSESLRRIAEELGIAVEALVLVDDSP
                     YERAEVEALLPGVRTLD"
     gene            3328..3600
                     /locus_tag="IE189_RS04495"
                     /old_locus_tag="GCM10010496_09130"
     CDS             3328..3600
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (12-81). E-value: 1.3e-09.
                     Score: 30.3. Matches aSDomain:
                     nrpspksdomains_IE189_RS04495_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132943.1"
                     /locus_tag="IE189_RS04495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09130"
                     /product="acyl carrier protein"
                     /protein_id="WP_189917998.1"
                     /sec_met_domain="PP-binding (E-value: 7.3e-07, bitscore:
                     25.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTRERAAVEREIRSMIAEAARLDDTVVAGLPADTDLFGPEIGL
                     TSLAGVTLLGGIDRRYGVDVAALDLSLDSLQSIATLTDFVAAHLQ"
     aSDomain        3364..3570
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS04495_PP-binding.1"
                     /evalue="1.30E-09"
                     /label="IE189_RS04495_PP-binding.1"
                     /locus_tag="IE189_RS04495"
                     /protein_end="81"
                     /protein_start="12"
                     /score="30.3"
                     /tool="antismash"
                     /translation="EIRSMIAEAARLDDTVVAGLPADTDLFGPEIGLTSLAGVTLLGGI
                     DRRYGVDVAALDLSLDSLQSIATL"
     gene            3747..4043
                     /locus_tag="IE189_RS04500"
                     /old_locus_tag="GCM10010496_09140"
     CDS             3747..4043
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132942.1"
                     /locus_tag="IE189_RS04500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09140"
                     /product="chaplin"
                     /protein_id="WP_189917999.1"
                     /transl_table=11
                     /translation="MSRIAKVAAVALGTSAVVVSGAGLAMADAGAQGAAVGSPGVLSGN
                     VVQVPVNIPVNVCGNTISVIGLLNPAFGNTCANVSDHHKGGDKHGEHGGGYGG"
     gene            complement(4151..5017)
                     /locus_tag="IE189_RS04505"
                     /old_locus_tag="GCM10010496_09150"
     CDS             complement(4151..5017)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="IE189_RS04505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09150"
                     /product="tyrosinase family protein"
                     /protein_id="WP_189918001.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTAAERRRFVKALLEIKRRGEYDEFVRMHIDHYVS
                     DGDGGLRSAHMAPSFLPWHRRFLLDLERALRRVDPSVTVPYWDWTRDRSTTAAPWTKDL
                     LGGNGRRSDHQVTTGPFAYSTGQWTIRENVTDGKFLTRDLGRAAAPIALPTGSDVEGAL
                     QDPVYDTAPWDSTATRGFRNKLEGWGTGSGSAAWRNHNRVHRWVGGVMLGGASVNDPVF
                     WLHHAFIDLLWTRWQTRHREHRYLPAAAPGPRDRQYRRVVARQEKLPPWDVTPAQLEDV
                     SRIYRYA"
     gene            complement(5001..5483)
                     /locus_tag="IE189_RS04510"
                     /old_locus_tag="GCM10010496_09160"
     CDS             complement(5001..5483)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136068.1"
                     /locus_tag="IE189_RS04510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09160"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_189918003.1"
                     /sec_met_domain="melC (E-value: 8.1e-49, bitscore: 161.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVDIDAGPVTAVPVGAGRRAAARATRRRDLLRGLLGATAALATV
                     PVVTASRPTRPADGGPADSSFDETYRGRHIHGVLAPTGQTAAGARWDITVDGRPLHLMR
                     RADGTWLSMVDHYRSYATPLEAARAAVDELGPGERLREADQPHQHTHMGGHHGLHP"
     gene            complement(5663..6184)
                     /locus_tag="IE189_RS04515"
                     /old_locus_tag="GCM10010496_09170"
     CDS             complement(5663..6184)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="IE189_RS04515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09170"
                     /product="hypothetical protein"
                     /protein_id="WP_189918005.1"
                     /transl_table=11
                     /translation="MKPTTRGTLAAVVTCVAAAAAAAASVTPAAAIGTVPVPVPLDGVE
                     KSLNVELPRLGAELPLPTPGAPEGPRFVEGRLLPERVVPQLPVAAGLPGVGLRAPLPEI
                     LGNDFNHLGLDAPASDVRTLGPGLTLDAPLTAPNPDRFGLPDLQLPAAGVLTPVLQAVP
                     GADLGLGQGL"
     misc_feature    6247..6258
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.43"
                     /tool="antismash"
     gene            complement(6320..6916)
                     /locus_tag="IE189_RS04520"
                     /old_locus_tag="GCM10010496_09180"
     CDS             complement(6320..6916)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132938.1"
                     /locus_tag="IE189_RS04520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09180"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_189918007.1"
                     /transl_table=11
                     /translation="MREPYPAPAGGGRGYALLLVLTGAAGLLAAWVITLDKFKLLEDPG
                     FTPGCSLNPVVSCGSVMTSHQASVFGFPNPMLGLVAYGIVVCVGMSLLAGAVFPRWYWL
                     TFAAGCLFGVVFVSWLQFQSLYRINALCLWCCLAWVATILMFWYVVSFAVRHAFLPAPA
                     WARTFLTEFTWVPPLLHIGVIGMLILTRWWDFWTS"
     gene            complement(6916..7416)
                     /locus_tag="IE189_RS04525"
                     /old_locus_tag="GCM10010496_09190"
     CDS             complement(6916..7416)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065676.1"
                     /locus_tag="IE189_RS04525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09190"
                     /product="DUF5949 family protein"
                     /protein_id="WP_189918009.1"
                     /transl_table=11
                     /translation="MTSTPSATRPFRVTDLGTLVVMPWSGEAPDGSDMPYLLAYSLGDT
                     QEGPEGTAAAVARLLTDNGMSVGGEVVDGTERPSLPFGLLVEAGAAVLNMPGLNAQCLP
                     PAEWLTAAEQRGYAYLVFTTRAWPEATPGRPVPPEALAAFAGAPETLTAAAHIVLPARR
                     LRG"
     gene            complement(7583..7870)
                     /locus_tag="IE189_RS04530"
                     /old_locus_tag="GCM10010496_09200"
     CDS             complement(7583..7870)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116485.1"
                     /locus_tag="IE189_RS04530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09200"
                     /product="hypothetical protein"
                     /protein_id="WP_189918010.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVLAGAAAPAFATPADLTPTSLNGAVDTLAKG
                     PINLRDAMPLQHQSDALDTENKDSVLNTVNGAAAALNAPGSLLGGLPLRG"
     gene            complement(8082..8948)
                     /locus_tag="IE189_RS04535"
                     /old_locus_tag="GCM10010496_09210"
     CDS             complement(8082..8948)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE189_RS04535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010496_09210"
                     /product="hypothetical protein"
                     /protein_id="WP_229901031.1"
                     /transl_table=11
                     /translation="MVCPHGVPLPAALCATLVLGIAVPAAMAADGAAAHERTAAVSDSP
                     VADADALLAQAARLGGFGTVLAPVTGLLDTVLKADDGRLTTDQAARLGAAVAAAVAKIT
                     ATAPATDPAPPADPAAQTPAQPAAPAHPAAQAPADPAVQAAAEPAASLSSSSSGGKSGA
                     AGKSTAAAGRAKAAADPVSDAVAALDKAVTALLAAATSPDVGQVLPAVSGVVSGLVDLL
                     TATPAGAGLTLPELPVQAPAVAPPALPAEASLLPAAPAAPAAPAVPEAPAAPAAPAAPA
                     APAAPAA"
     gene            complement(9231..9470)
                     /locus_tag="IE189_RS04540"
                     /old_locus_tag="GCM10010496_09220"
     CDS             complement(9231..9470)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="IE189_RS04540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_09220"
                     /product="chaplin"
                     /protein_id="WP_189918012.1"
                     /transl_table=11
                     /translation="MSRIAKGLALTSVAAAAVVGASGLAAADSGANGVAEHSPGVLSGN
                     VVQVPVHIPVNVCGNTINVVGLLNPAFGNTCVND"
     misc_feature    complement(9493..9502)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: medium, score: 15.86"
                     /tool="antismash"
     misc_feature    9665..9680
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.02"
                     /tool="antismash"
ORIGIN
        1 accgtcccgg aatatcgtaa agctcctcgc taaagcaccg agaaactcgc cgcagaacag
       61 caagacgaac aggcagccga aggggcagcc gaacaggtag ccgaaggggc agccgaacag
      121 gtagcagaac gggcaggaga accctccgcc gcatatccga atgcgccgcg agcgatttcc
      181 ctttcccgcg actccctttc cgtatttccc cgctcccgga gcgggtcctg atggctgctc
      241 accagcccgt atactcgata gtcccggaat ccttcaatca gtttgcctgg agcagcattg
      301 tgacgaacgc gcgtcggcac cctcggagca ccaccgcgga ttccgtgctc acggaagtcg
      361 cggaagccgt ccgtctccag gagggcccgc cgggagtacg gtccgtcctt cgtgccctgc
      421 gtcggctgga gccggcgtcc accaaggacc tcagccgcgc gaccggactg cccgtcccga
      481 tcgtggccgc gctgggcaac gaactgcgcc gccgggggtt cgtcaccgcg cagcgtccgt
      541 cccggctgac cgaggacggt cgcgacctgg tcgccgagct cggcatggac ctctcgctcg
      601 acgccacctg ccgcacgtgc gacgggcgcg agctcgtgat cccggacgtg ctggacgagg
      661 cggtgcgccg gctgcgggcg ctgatggagt cgggtcccgc cgtcgacatg gcgatcgacc
      721 agtcgcactg cacggccgag accaaggtgc gccgggtgct cgcgctgctc agggcgggcg
      781 cgctgccggg cggttcgctg ctgctgatcg gcgacgacga cctggtgtcc ctcgccgtcg
      841 ccgtggtggg tgatgtgctc ggcggtccga tcgtcgagcg ggtgaccgtc gtggacatct
      901 ccccggagat cctcgactac atccagaagg tgtcggccgg tctcggcacc cgtgtggaga
      961 ccgtgcgcca cgacctgcgg ctgccgctgc ccgaggcact ccaccagcag cacgacgtgg
     1021 cgatgaccga cccgccgtac acccccgaag gcgcccgcct gttcctgtcg cgggccgtgg
     1081 aggggctgcg gcccggtccc gcgcacagca tcttcttctc cttcggcggc aagagccccg
     1141 acgagatgct ggaggtgcag cgggagatca tcgggctcgg cctgatcacc aacgggtaca
     1201 tccgcaactt caacgagtac gagggcagcg gcatcctcgg gggtgtcggc ttcttccagc
     1261 acctgctcac caccacctcg acggccccct cccaggacgg cgagttcagc ggcccgctct
     1321 acaccgggga caagcggacc cggcagcgcg agtacacgtg cgtggcgtgc gacgcgcggc
     1381 tgcgcgtcgg gcccggcgcg cgctgggcct cggtcggcgc cctgcgcgag gaaggctgcc
     1441 cgaactgcgg caagggcccc ttccgtccgg gccgcctggt cccggcggag gatccggccc
     1501 cctcggccga gcagccggcc accccggtca cggccgctgg cggccccgcc gatgccggcc
     1561 cccctgtcac tcccaccacg cccgccaccc ccgacgctcc cgtctccgcg tctcccgggg
     1621 cctccgcccc cgcgccttcc gtcgggcggg cccacggctg gcgggcgccg agcgaggccg
     1681 agcgggccgc gctcgccgag cgggcacggc cgtacgtcac ccgtggggcc gacgagcgcg
     1741 acctcccggc catcgggcgg ttcgaggccg agatcgcccg ggtctccttc ggggaggacg
     1801 ccatcgacga cccgcagcgg tgggcgtccc ggctcgggcg cgccatggag aagtcgaagg
     1861 agggcatgat cgtcgccgac gctccgggcg gggagcccgt gggctggtgc tgggtgagca
     1921 tcaaccagaa cgcgatgacc ggcgaccgtt acgccaactt ccgttccctc gcggtgtccc
     1981 cggtggacaa ccgcggtgac gtcgccgaac tcctcctcac cgcgggcctg gagttctgcc
     2041 tggccaacgg catcaccgag gtcgtcggcc gggtccacgt gggcaacgtc ccgatgcgca
     2101 cggtgtaccg gaagttcggg ttcgacccca cgagcctgtc gatgaagctg ttcctgcccc
     2161 aggggaccga cggccgatga gcgtggcgga cggtttcgat cccgacggcg tgaagtgcgt
     2221 cgtctgggac ctcgacggca ccctgtggga cgagatcgcc gtcgagtcgc ccaccgacga
     2281 cctgccggcg ccccgccccg cgacgctcgc cgcgatcgac gacctggcgg cccgcggtgt
     2341 gctcagcagc atcgcgagcc gcagcgctcc ctcggtgctc gaccggctgg acgccgtacc
     2401 ggggctgcgg gcgcgtttcc tcgcgccgca ggtgtcctgg caggacaaga gcgagtcgct
     2461 gcgcaggatc gccgaagaac tcggcatcgc ggtggaggcc ctcgtcctgg tcgacgactc
     2521 cccctacgag cgcgccgagg tcgaggcgct cctgcccggg gtgcgcaccc tggaccccgc
     2581 ggacgtgccc gcgctgctgg ccgcgttcga ggggcgggag atcacccccg agtcccgcgc
     2641 ccgcgtgggc cgttaccgca cggaggagac ccgcagggcc gagggcgcgc ggttccgggg
     2701 ctcgcgtgag gagttcctgc gctggtgcga catgcggctg accgtcggcg cggccaccgc
     2761 cgacgagctc ccgcgcgccc tggaactcgc cgcccgcacc caccgcctga actcctccgg
     2821 gctcaccccg gaccggctgc gcgagctggc cgcggcggac ggacacgagc tgtacaccgc
     2881 ccgcatgacg gaccgtttcg gcgactacgg catcatcggc gccgccctcg tcgaccgcgg
     2941 cccggacacc cccggcgtgc gcgacggcgc cgggacggct tccgccacct gggccgtccc
     3001 gctgctcgcg ctctcctgcc gggtcgcggg gcggggcgcg gcggccgcct tcctgttccg
     3061 gctgatggaa cgggcccgcg aggccggcgc cgaggagttc cgggtgacgc tgcgcccgac
     3121 ggacgccaac ctggagatgc gcatcctgct ccgccaggcc ggcctgcgcc gcacggacgc
     3181 cgccgtgccc ggcccggccg acgcgtccgg tgcccccgtc gctcggacgg cccccgccga
     3241 ggcggcggtc ctgggccgga cactcggggg cgatgtgccc gggccgcccg cctggctgca
     3301 cgtatccgac cgagaggaca ccgaagcgtg acggacaccc gagagcgggc cgcggtggaa
     3361 cgggagatcc gttcgatgat cgccgaggcc gcccgtctgg acgacaccgt ggtcgccgga
     3421 ctgcccgccg acaccgacct gttcggaccg gagatcggac tgacctcgct ggccggggtg
     3481 accctgctgg gcgggatcga ccggcgctac ggcgtcgacg tggccgcgct ggacctcagc
     3541 ctggacagtc tccagtccat cgccaccctg acggacttcg tggcggcgca cctccagtag
     3601 cactagccac ccggctgtac gtccgtcctg ttcaaaaagg gtgatccggc cggacgcacc
     3661 gtgacccgcc cggggtggcc gcgttgaacg gggagtgcgg tggcgacccc tgccgccgtg
     3721 cgaaccgatt ccctaaggag aacgtgatgt ctcgcatcgc gaaggtggcc gctgttgccc
     3781 tcggcaccag cgccgtggtg gtcagcggag ccggcctggc catggcggac gcgggtgcgc
     3841 agggcgcggc cgtgggttcg ccgggtgtcc tgtcgggcaa cgtcgtccag gttccggtca
     3901 acatcccggt caacgtctgc ggcaacacca tcagcgtcat cggcctgctg aacccggcct
     3961 tcggcaacac ctgcgccaac gtcagcgacc accacaaggg tggcgacaag cacggcgagc
     4021 acggcggcgg ctacggcggc tgacccccgc ccgtacgtcc cgtacgtaca ccccggtcgt
     4081 accccgtgta cggaccacca ggagagcccc ggcacctcga gcgccggggc tctccccgtc
     4141 cgtcaccggt tcacgcgtac cggtagatcc gggagacgtc ctcgagctgg gccggggtca
     4201 cgtcccacgg ggggagtttc tcctgccggg cgacgacccg gcggtactgg cggtcgcgcg
     4261 ggcccggcgc cgcggcgggc aggtagcggt gctcgcggtg gcgggtctgc cagcgcgtcc
     4321 acagcaggtc gatgaaggcg tggtgcagcc agaacaccgg gtcgttgacg gaggcgccgc
     4381 cgagcatgac cccgcccacc caccggtgca cccggttgtg gttgcgccag gcggcgctgc
     4441 ccgagccggt cccccagccc tccagcttgt tgcggaagcc ccgggtggcc gtcgagtccc
     4501 agggcgcagt gtcgtagacc gggtcctgga gcgccccttc cacgtcgctc ccggtgggca
     4561 gggcgatcgg ggcggcggca cggccgaggt cgcgggtgag gaacttcccg tcggtgacgt
     4621 tctcccggat ggtccactgg ccggtggagt aggcgaacgg ccccgtggtc acctggtggt
     4681 cggagcgccg tccgtttccg ccgaggaggt ccttggtcca gggcgccgcg gtcgtggagc
     4741 ggtcgcgcgt ccagtcccag tagggcacgg tcaccgaggg gtccacccgg cgcagcgccc
     4801 tttccaggtc gagcaggaac cggcggtgcc agggcaggaa ggagggcgcc atgtgggcgg
     4861 accgcaggcc tccgtcgccg tcggagacgt agtggtcgat gtgcatgcgg acgaactcgt
     4921 cgtactcccc cctgcgtttg atctccagca gcgccttcac gaagcgccgc cgctcggcgg
     4981 cggtgagtgt gctgacgtcc ttacgggtgt aggccatggt gtccccccat gtgcgtgtgc
     5041 tggtgcggtt ggtccgcctc gcgcagccgt tccccggggc cgagttcgtc cacggcggca
     5101 cgggccgcct ccaggggggt ggcgtacgag cggtagtggt cgaccatgct gagccaggtg
     5161 ccgtcggcgc ggcgcatgag gtgcagcggc cggccgtcca ccgtgatgtc ccagcgggcg
     5221 cccgccgccg tctgtcccgt cggggcgagg accccgtgga tgtgccgtcc gcggtaggtc
     5281 tcgtcgaagg aggagtccgc ggggccgccg tccgccggtc gggtgggccg ggaggcggtg
     5341 acgacgggga cggtcgcgag ggccgcggtg gcgccgagca gcccgcgcag caggtcccgt
     5401 cgccgggtgg cgcgcgccgc ggctcgtctg ccggctccca ccggtaccgc ggtcaccggt
     5461 cctgcgtcga tgtcgacggc catggtgtgc tcctcgtccg gtctgcgtgg cgggtgtccg
     5521 tggtggtaac cgtccgacgg ccctccgggt caccgcccgg gggccggacg gtcggcgcgg
     5581 gaagcggatc cgggcatggc gacgggcccg gacgacgccg tccgggcccg tggtcgtggc
     5641 ggtggggccg ccggtcggcc ggttagagac cctggcccag gccgaggtcc gcgccgggca
     5701 ccgcctggag caccggggtg agcacgcccg ccgcgggcag ctggaggtcc ggcaggccga
     5761 agcggtcggg gttgggcgcc gtcagcgggg cgtccagggt gagccccggg ccgagggtgc
     5821 gcacgtcgga ggcgggggcg tccagtccga ggtggttgaa gtcgttgccg aggatctccg
     5881 gcaggggtgc gcgcagcccc acgccgggca gcccggcggc gacgggcagc tgcgggacga
     5941 cgcgttcggg gagcagccgg ccctcgacga aacgcggtcc ctcgggcgcg cccggcgtcg
     6001 gcagggggag ctcggcgccg agcctgggca gttccacgtt gagggacttc tcgactccgt
     6061 ccagcgggac ggggacgggc accgtgccga tcgcggcggc gggggtcacg gaggcggcgg
     6121 cagcggcggc ggccgcgaca caggtgacga cggcggcgag ggttcctcgg gtggtcggct
     6181 tcatctcggg tacgggacct ttcgcaggga ttcgggggcg ttgcgtccgt accgggtaac
     6241 gagccggaaa acggcatcag ttcacaattc ccccgggtgc cgcaatagtg ctacggccgc
     6301 cggtacgacg gtcggaaggt cagctcgtcc agaagtccca ccaccgggtc aggatcagca
     6361 tgccgatcac cccgatgtgc agcagcggcg gcacccaggt gaactcggtg aggaaagtcc
     6421 gtgcccaggc aggcgcgggc aggaacgcgt ggcgtacggc gaacgagacg acgtaccaga
     6481 acatgaggat cgtggcgacc caggccagac agcaccacag gcacagcgcg ttgatccggt
     6541 acagggactg gaactggagc caggacacga acacgacgcc gaagaggcag cccgcggcga
     6601 aggtcagcca gtaccagcgg gggaagacgg cgccggccag caggctcatg ccgacgcaga
     6661 cgacgatccc gtaggccacc aggcccagca tcgggttggg gaacccgaac accgaggcct
     6721 ggtggctcgt catgacgctg ccgcaggaga cgacggggtt caggctgcac ccgggcgtga
     6781 agccgggatc ctccagcaac ttgaacttgt cgagggtgat gacccaggcg gccagcagcc
     6841 cggccgcacc ggtcaggacg agcagcaggg cgtacccgcg gccgccgccc gccggcgccg
     6901 ggtacggctc gcgcatcagc cgcgcagtcg gcgcgcgggc aggacgatgt gggccgcggc
     6961 cgtcagggtc tcgggggcac ccgcgaacgc ggccagcgcc tcgggcggga cgggccggcc
     7021 gggcgtggcc tcgggccagg cgcgggtggt gaagacgagg taggcgtagc cgcgctgctc
     7081 ggcggcagtc agccactccg cgggcggcag gcactgggcg ttcaggcccg gcatgttgag
     7141 gacggcggca ccggcctcga cgagcaggcc gaacggcagg ctgggccgct cggtgccgtc
     7201 gacgacttcg ccgccgacgg acatgccgtt gtcggtgagc aggcgcgcga cggcggctgc
     7261 cgtcccctcc ggtccttcct gggtgtcgcc gagggagtag gccagcaggt agggcatgtc
     7321 actgccgtcg ggggcctcgc cgctccacgg catcacgacg agggtgccca ggtcggtcac
     7381 gcggaagggg cgggttgcgc tgggggttga ggtcaccgcg cgaccctacc ggcgcggccg
     7441 ggcgccttcc ggtgtgttct cacccgaccg gatgacgtgg gcgggttgct ccacacgaac
     7501 gagggacgcg ccgctcgacc cgcctcgaac agcgacgggg ccggtcccgt cggtgacgga
     7561 ccggccccgc gcgaggcgcg tctcagccgc ggagcggcag cccgccgagg agggagccgg
     7621 gggcattcag ggcggcggcc gcgccgttga cggtgttgag gacggagtcc ttgttctcgg
     7681 tgtcgagggc gtccgactgg tgctggagcg gcatcgcgtc ccgcaggttg atcggaccct
     7741 tggcgagggt gtccacggcg ccgttgaggc tggtgggcgt gaggtcggcg ggggtcgcga
     7801 acgcgggtgc ggcggcaccg gcgaggacca gggatccggc aacgacagcg gcagccttca
     7861 gggacttcat cgggttcctt tcttcggcga cccgccgacc cggcccggta cggcggctcg
     7921 gccgtccgcg tgtcgggtcg tgcggcggct cggcgcggca cgggtcgcca gggacgttct
     7981 gggcgccgtg cctaacgagc cccgccggat gcggaaaccg tgtgcgcaga aatttccgca
     8041 ccccggcgca gcggccgggg tgcggggaca cggggcgggg ctcaggcggc gggcgcggcc
     8101 ggtgctgcgg gggctgccgg tgctgcgggg gctgccggtg cctcggggac cgcaggggcc
     8161 gcgggggcgg ccggggccgc ggggaggagc gacgcctcgg ccggaagcgc gggcggcgcc
     8221 accgccgggg cctggaccgg cagttcggga agggtcaggc cggctccggc cggcgtggcg
     8281 gtgagcaggt cgacgaggcc ggacaccacg ccggacacgg ccgggaggac ctggccgacg
     8341 tcgggggagg tggcggcggc gagcagggcg gtgaccgcct tgtcgagcgc ggcgacggcg
     8401 tcggacaccg ggtcggcggc cgccttcgcc cggccggcgg ctgcggtgct cttgcccgcc
     8461 gcaccgctct tgccgcccga ggaggacgag gacagcgagg ccgcgggctc ggcggcggcc
     8521 tgcaccgcgg ggtcggcggg cgcctgcgcc gccgggtggg ccggggcggc gggctgggcg
     8581 ggcgtctggg ccgcggggtc ggcgggtggg gccgggtccg tcgccggggc cgtcgccgtg
     8641 atcttggcga cggccgccgc cacggccgcg ccgagccggg cggcctggtc ggtggtgagc
     8701 cggccgtcgt cggccttgag gacggtgtcg agcaggccgg tgaccggggc gaggaccgtg
     8761 ccgaagccac cgagccgggc ggcctgggcg agcagcgcgt ccgcgtcggc gacgggcgag
     8821 tccgacaccg cggcggtgcg ctcatgggca gcggccccgt cggccgccat ggcggccggg
     8881 acggcgatgc cgaggacgag ggtggcgcag agtgcggctg gaagcggtac gccgtgcggg
     8941 cagaccacgc atgggagttc ctctcggtcg cgtcgggtct cccgcccacc gtgcgaacgg
     9001 gtgtagccct ccgcaaccgt tcgaccgctc gaggtaatca ccgcaggccg gtcccgcctg
     9061 catcacccct ggtggggcgg catggcaaga ccccgcgcgg ggtgcggccg ccttgccccg
     9121 ttcgaggcga tcaccccgac gggcgcccgc acgaaggcga acggccgccc tcccgactgg
     9181 agggcggccg ttcggctcat gacgtgtcgt gacccctggg ggaggtgacg tcagtcgttg
     9241 acgcaggtgt tgccgaacgc cgggttcagc aggccgacca cgttgatcgt gttgccgcag
     9301 acgttgaccg ggatgtgcac gggaacctgg acgacgttgc cggacagcac gcccggggag
     9361 tgctcggcga cgccgttcgc gccactgtcg gcggcggcga ggccggaggc gccgacgacg
     9421 gccgcggcgg caacggaggt cagggccagg cccttcgcga tacgcgacat ggagaggtgc
     9481 tccttggggt gatgaaaaaa cacccgggcg gggatgcccg gcgctgaatc aacgcgcggg
     9541 gctcgcgggg gttgtgccgc cgaacgagtg atgtcaccgg agcccggctt caccattccg
     9601 acacacttgt ccggtttcga cggattagtc cggtcagcga ttagagttgc gccccatccg
     9661 acgcacgcct atagcaagca aatcgcacga aagggccccg ccggtcatgc gcaattccct
     9721 tggtccgctg ctacgccgcg cgaccgtcgg cgtcctcgcc ctcgcgtcgc tctgggcgcc
     9781 cgccggagcc ctcgcggccg gcgcgccgcc cgccgccgag gctccgcgcc tcgccttcac
     9841 ccagcgcttc cacgcgacac agcacggcgg gatcgtccgc gccgccaacg cggccatcag
     9901 ctgccgcacc acgacgacgc ccaccgggaa ggcggcgccg acctgcgctt ccgtgcgcca
     9961 gggcacggcc ggcgtcaacc aggccttcaa catgttctac gtcgacgtcg acaccgaccc
    10021 gaacacctac aactcctccc gcgcggaggt ccgtctgccc cggggcgcgc gggtgtcgta
    10081 cgcgcggctg tactggggcg gcaatctcct ggtcggcgag cagaagccgc cgaaggacaa
    10141 cgggcgcgtg ctgatcgccg aacccggtgg cgcgtacaag gcggtcctcg cggacacggt
    10201 cgtcggccac cgcgtggagc ggggcgccga cgccttccag gcctccgcgg acgtcaccgg
    10261 actggtccgc cgcagcggcg gagggctgta cacggtcgcg caggtcaatg tggccatggg
    10321 caggtccgcg gccggcgcgt ggggcggctg gaccctggtc gtggcctacg agaacccggc
    10381 cgagccgctg cggcaccttt ccctgtggga cggcttcgac accgtcggcg ccgccggcaa
    10441 ggagctccgg ctgcgcggac tgcgcatccc ccagggggcg ggc
//
