LOCUS       NZ_BMRD01000051         3110 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence51, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000051
VERSION     NZ_BMRD01000051.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3110
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3110
                     /culture_collection="JCM:3295"
                     /db_xref="taxon:46158"
                     /mol_type="genomic DNA"
                     /organism="Actinomadura citrea"
                     /strain="JCM 3295"
                     /submitter_seqid="sequence51"
                     /type_material="type strain of Actinomadura citrea"
     gene            complement(<1..>3110)
                     /locus_tag="IEZ14_RS41165"
     region          1..3110
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3110
                     /SMILES="NC(C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3110
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3110](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3110)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..3110)
                     /NRPS_PKS="Domain: AMP-binding (1-309). E-value: 2.2e-71.
                     Score: 232.5. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41165_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (413-482). E-value: 3.5e-31. Score:
                     99.2. Matches aSDomain: nrpspksdomains_IEZ14_RS41165_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (498-813). E-value:
                     4.4e-115. Score: 375.7. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41165_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852241.1"
                     /locus_tag="IEZ14_RS41165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_189269996.1"
                     /sec_met_domain="AMP-binding (E-value: 6.6e-86, bitscore:
                     274.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.8e-19, bitscore:
                     56.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.1e-68, bitscore:
                     217.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVAYMLEDAAPACVITTSGADLPGTTPRVLLDALDLAEHPAVSPS
                     DADRVRPLRPGNAAYVIYTSGSTGRPKGVVVSHANAVDLCAWAGKDFGPERLARVLFST
                     SLNFDVSVFEWLTPLTAGGHIEVVRDLLEVAERGGWSGTLISGVPSAMSALLATGALTL
                     DAGDVVLAGEALPARLVQDLRALLPDARISNIYGPTEATVYAAAWFDDGNAAGHAPIGG
                     PIANTRAYVLDASLRPVPAGVPGELYLAGEGLARGYLRRPGLTADRFVACPFGEPGGRM
                     YRTGDLVRWDERGRIEYLGRLDHQVKVRGFRIEPGEIETALSAHPAVTQSVVVARRDNA
                     GDTVLVAYVVSDGADPADLKRFVAAVLPEYMVPSAIVVLDAMPLNPNGKLDRAALPEPD
                     LAAPAGGRRPRDAREEALCAVFAEVLGVERVGIDDNFFDLGGDSLKATRVVSRARAALG
                     ADLGVRALFEAPTVAALAAVLKEADGDARPRLVPAERPPLVPVSYAQRRLWFIHDLEGP
                     NATYHIPIPLRLTGELDVAAVRAAMRDMMARHEALRTVFVQVEGEPYQRILTPDAMARE
                     PEFTDLSPFRGAGEGTPPQGDVTPEDLHAAVFEAATRGFDLAAEPPVRAHVFRLAEDDH
                     LLLLVMHHIAGDGWSMEPLAADLITAYAARAAGDAPRWEPLPVQYADYALWQREVLGGE
                     DDPGSVIAAQLAYWREALAGLPEELALPADRPRPPRATYRGAQHPFTLPPALHEALAAL
                     GRDHRASLFMVLQAALAGLFTRLGAGTDVPIGSPIAGRTDEALNDLVGVFVNTLVLRTD
                     TSGDPTFAELLARVREADLAAFAHQDVPFERLVEVLNPQRSMARHPLFQAMISIQNDPS
                     ARVALPGLTVALEPVDPGVSRFDLALLVDETRDASGAPAGIACRLEYALDLFDPETAAS
                     MAARLERLLAQVAADPSLPLSAVDLLDDAERDTLLRGWNATDLPVAPAVVPELVEAQAA
                     RTPEAAAVVAGGARLTYAELNARANRLARHLIARGVGAEDFVALALPR"
     CDS_motif       complement(522..632)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0009"
                     /evalue="4.90E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="863"
                     /protein_start="826"
                     /score="67.2"
                     /tool="antismash"
                     /translation="READLAAFAHQDVPFERLVEVLNPQRSMARHPLFQAM"
     aSDomain        complement(672..1616)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41165_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.40E-115"
                     /label="IEZ14_RS41165_Condensation_LCL.1"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="813"
                     /protein_start="498"
                     /score="375.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAGDG"
                     /tool="antismash"
                     /translation="VPVSYAQRRLWFIHDLEGPNATYHIPIPLRLTGELDVAAVRAAMR
                     DMMARHEALRTVFVQVEGEPYQRILTPDAMAREPEFTDLSPFRGAGEGTPPQGDVTPED
                     LHAAVFEAATRGFDLAAEPPVRAHVFRLAEDDHLLLLVMHHIAGDGWSMEPLAADLITA
                     YAARAAGDAPRWEPLPVQYADYALWQREVLGGEDDPGSVIAAQLAYWREALAGLPEELA
                     LPADRPRPPRATYRGAQHPFTLPPALHEALAALGRDHRASLFMVLQAALAGLFTRLGAG
                     TDVPIGSPIAGRTDEALNDLVGVFVNTLVLRTDT"
     CDS_motif       complement(681..770)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0008"
                     /evalue="1.80E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="810"
                     /protein_start="780"
                     /score="42.8"
                     /tool="antismash"
                     /translation="DVPIGSPIAGRTDEALNDLVGVFVNTLVLR"
     CDS_motif       complement(1038..1076)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0007"
                     /evalue="2.00E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="691"
                     /protein_start="678"
                     /score="20.2"
                     /tool="antismash"
                     /translation="QYADYALWQREVL"
     CDS_motif       complement(1125..1190)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0006"
                     /evalue="9.00E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="662"
                     /protein_start="640"
                     /score="31.0"
                     /tool="antismash"
                     /translation="MHHIAGDGWSMEPLAADLITAY"
     CDS_motif       complement(1440..1556)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0005"
                     /evalue="1.80E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="557"
                     /protein_start="518"
                     /score="55.7"
                     /tool="antismash"
                     /translation="ATYHIPIPLRLTGELDVAAVRAAMRDMMARHEALRTVFV"
     aSDomain        complement(1665..1871)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41165_PCP.1"
                     /evalue="3.50E-31"
                     /label="IEZ14_RS41165_PCP.1"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="482"
                     /protein_start="413"
                     /score="99.2"
                     /tool="antismash"
                     /translation="EEALCAVFAEVLGVERVGIDDNFFDLGGDSLKATRVVSRARAALG
                     ADLGVRALFEAPTVAALAAVLKEA"
     aSModule        complement(1665..3107)
                     /complete
                     /domains="nrpspksdomains_IEZ14_RS41165_AMP-binding.1"
                     /domains="nrpspksdomains_IEZ14_RS41165_PCP.1"
                     /locus_tags="IEZ14_RS41165"
                     /monomer_pairings="Ala -> Ala"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2154..2219)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0004"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="319"
                     /protein_start="297"
                     /score="39.4"
                     /tool="antismash"
                     /translation="YLGRLDHQVKVRGFRIEPGEIE"
     aSDomain        complement(2184..3107)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41165_AMP-binding.1"
                     /evalue="2.20E-71"
                     /label="IEZ14_RS41165_AMP-binding.1"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="309"
                     /protein_start="1"
                     /score="232.5"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="VAYMLEDAAPACVITTSGADLPGTTPRVLLDALDLAEHPAVSPSD
                     ADRVRPLRPGNAAYVIYTSGSTGRPKGVVVSHANAVDLCAWAGKDFGPERLARVLFSTS
                     LNFDVSVFEWLTPLTAGGHIEVVRDLLEVAERGGWSGTLISGVPSAMSALLATGALTLD
                     AGDVVLAGEALPARLVQDLRALLPDARISNIYGPTEATVYAAAWFDDGNAAGHAPIGGP
                     IANTRAYVLDASLRPVPAGVPGELYLAGEGLARGYLRRPGLTADRFVACPFGEPGGRMY
                     RTGDLVRWDERGRIEYLGRLDHQVKVR"
     CDS_motif       complement(2304..2393)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0003"
                     /evalue="3.70E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="269"
                     /protein_start="239"
                     /score="51.2"
                     /tool="antismash"
                     /translation="PAGVPGELYLAGEGLARGYLRRPGLTADRF"
     CDS_motif       complement(2850..2888)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0002"
                     /evalue="1.30E+01"
                     /label="NRPS-E2"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="87"
                     /protein_start="74"
                     /score="-1.0"
                     /tool="antismash"
                     /translation="VVSHANAVDLCAW"
     CDS_motif       complement(2877..2936)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41165_0001"
                     /evalue="2.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="IEZ14_RS41165"
                     /protein_end="78"
                     /protein_start="58"
                     /score="35.1"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVVSH"
ORIGIN
        1 cgcggcagag ccagcgcgac gaagtcctcc gcgccgacac cccgcgcgat caggtggcgg
       61 gcgaggcggt tcgcgcgggc gttcaactcg gcgtaggtga gccgcgcccc gcccgcgacc
      121 acggcggccg cctcgggcgt ccgggcggcc tgcgcctcga ccagttcggg gacgacggcc
      181 ggggcgacgg gcaggtcggt cgcgttccag ccgcgcagga gggtgtcgcg ctcggcgtcg
      241 tccagcaggt cgacggcgct gagcggcagg gacgggtccg cggcgacctg cgccagcagc
      301 cgctccagcc gggccgccat cgacgcggcg gtctcggggt cgaacaggtc gagcgcgtac
      361 tccagccggc aggcgatccc ggcaggggcg ccggaggcgt cccgcgtctc gtccaccagg
      421 agcgcgaggt cgaagcggga cacgcccggg tcgaccggct ccagcgcgac cgtcaggccg
      481 ggcagcgcga cccgcgcgga cgggtcgttc tggatcgaga tcatcgcctg gaacagcggg
      541 tgccgggcca tcgaccgctg cgggttcagc acctcgacca gccgctcgaa cgggacgtcc
      601 tggtgggcga acgcggcgag gtcggcctcg cggacgcggg cgagcagctc ggcgaacgtc
      661 gggtcccccg aggtgtcggt gcgcaggacg agcgtgttga cgaacacgcc gaccaggtcg
      721 ttcagggcct cgtcggtgcg gcccgcgatg ggcgagccga tcggcacgtc cgtcccggcg
      781 ccgagccggg tgaacaggcc ggcgagcgcg gcctgcagca ccatgaacag gctggcgcgg
      841 tggtcgcggc cgagcgcggc cagcgcctcg tgcagggcgg gcgggagcgt gaaggggtgc
      901 tgggcgcccc ggtaggtggc gcgcggcggg cgcggccggt cggcggggag ggccagctcc
      961 tcgggcaggc ccgcgagggc ctcgcgccag taggccagct gcgcggcgat gacgctgccc
     1021 gggtcgtcct cgccgccgag gacctcccgc tgccagaggg cgtagtcggc gtactggacg
     1081 ggcagcggct cccagcgcgg cgcgtccccg gcggcgcggg cggcgtaggc ggtgatcagg
     1141 tcggcggcga gcggctccat cgaccagccg tccccggcga tgtggtgcat caccagcagg
     1201 agcaggtggt cgtcctcggc gaggcggaac acgtgcgcgc ggacgggcgg ctcggcggcc
     1261 aggtcgaacc cgcgcgtcgc ggcctcgaac accgccgcgt ggaggtcctc cggcgtgacg
     1321 tctccctgtg gaggggtgcc ctcccccgcg ccccggaagg gggacaggtc ggtgaactcc
     1381 ggttcacgcg ccatcgcgtc cggcgtgagg atccgctggt acggctcccc ctcgacctgc
     1441 acgaacaccg tgcgcagcgc ctcgtggcgc gccatcatgt cgcgcatcgc ggcgcggacc
     1501 gcggcgacgt ccagctcgcc ggtcagccgc agcgggatcg ggatgtggta ggtcgcgttc
     1561 gggccttcga ggtcgtggat gaaccacagc cgccgctgcg cgtacgacac gggcacgagg
     1621 ggcgggcgct cggcggggac gagcctcggc cgggcgtcac cgtcggcctc cttgagcacg
     1681 gcggcgagcg cggcgacggt cggcgcctcg aacagcgcgc ggacgcccag gtcggcgccc
     1741 agcgcggcgc gggcccgcga caccacgcgg gtcgccttga gggagtcgcc gccgaggtcg
     1801 aagaagttgt cgtcgatgcc cacccgctcg acgccgagga cctcggcgaa cacggcgcac
     1861 agcgcctcct cgcgggcgtc gcgcggccgg cgcccgcccg cgggcgcggc caggtcgggc
     1921 tccgggagcg cggcccggtc gagcttgccg ttcgggttca gcggcatcgc gtccaggacg
     1981 acgatcgccg acgggaccat gtactcgggc agaacggcgg ccacgaaccg cttaaggtcg
     2041 gcggggtccg ccccgtccga cacgacgtag gcgacgagga cggtgtcgcc cgcgttgtcc
     2101 cggcgcgcga cgaccacact ctgcgtgacg gccgggtgcg cggacagcgc ggtctcgatc
     2161 tcgcccggct cgatccggaa gccgcgcacc ttgacctggt ggtcgaggcg gccgaggtac
     2221 tcgatccggc cccgctcgtc ccagcggacg aggtcgcccg tccggtacat ccgcccgccc
     2281 ggctccccga aggggcaggc gacgaaccgg tcggcggtca ggccggggcg gcgcaggtag
     2341 ccgcgcgcga ggccctcgcc cgcgaggtac agctcgccgg gcacgcccgc cggaacgggc
     2401 ctcagggacg cgtccagcac gtaggcgcgg gtgttggcga tcgggccgcc gatcggggcg
     2461 tgtccggcgg cgttgccgtc gtcgaaccag gccgcggcgt agacggtcgc ctcggtcggg
     2521 ccgtagatgt tggagatccg cgcgtccggc aggagggcgc gcagatcctg gacgagccgc
     2581 gccggcagcg cctcccccgc cagcaccacg tcccccgcgt ccagagtgag cgcgccggtg
     2641 gccagcagcg cggacatcgc cgacgggaca ccgctgatca gcgtcccgga ccagccgccg
     2701 cgctcggcga cctccagcag gtcgcggacg acctcgatgt ggccgcccgc cgtgagcggc
     2761 gtcagccact cgaacaccga cacgtcgaag ttgagcgacg tggagaacag cacgcgggcg
     2821 agccgctccg ggccgaagtc cttccccgcc caggcgcaca ggtcgacggc gttggcgtgg
     2881 gagacgacga cgcccttggg gcgtcccgtc gacccggacg tgtagatgac gtaggcggcg
     2941 ttgcccggcc ggagcggccg gacacggtcg gcgtcggacg ggctcaccgc cgggtgctcg
     3001 gccaggtcga gggcgtccag caggacgcgc ggggtggtgc cgggcaggtc ggcgccggac
     3061 gtcgtgatga cgcaggccgg agccgcgtcc tccagcatgt aggcgacacg
//
