LOCUS       NZ_BMRD01000049         4221 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence49, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000049
VERSION     NZ_BMRD01000049.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4221
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..4221
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1669:>4221](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1670..>4221
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..1677
                     /locus_tag="IEZ14_RS41130"
     CDS             <1..1677
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (0-60). E-value: 1.4e-18. Score:
                     58.9. Matches aSDomain: nrpspksdomains_IEZ14_RS41130_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (78-384). E-value: 1e-105.
                     Score: 346.1. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41130_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (390-529). E-value: 9.2e-30.
                     Score: 95.5. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41130_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IEZ14_RS41130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_229810787.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-13, bitscore:
                     38.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2e-55, bitscore:
                     175.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AGLWSEVLGVSEIGVHDNFFTLGGDSITSIVVMSRAARRGLRITP
                     WQFFENQTVAGQARVAVPVVAVEDRAGSAAPAAGPAPLTPVQRWFFDQAFSHPGHYNQG
                     WLFRVPATVDADRLAAAITGLLERHDALRLRFPRDTRAAGGRRAEYTAPGAAPAPAAFR
                     RVDLSGSAQWEPALARALADAQDIDLERDPPLRALLIDGGREDRCRLALIAHHLVIDAV
                     SWRILLDDLREAYRALAAGGEAGITDATTSFASWARALTELPGAVADDLEFWRAQRPSG
                     RWEVPLDRQASGAGTYGDAEDVEGVLGAEETTVLLRDLPAIHHSRVDEVLLTALALAMT
                     KSFGITRLYVDVEGHGREQHLVEGADLSRTVGWFTAIAPLLLRLPDGSAPGDALAAVKE
                     QMRAVPHGGLGHGILRHLDPVRGQALAEPPAPQVSFNYLGQFDASAETRTTRPPGDHLD
                     LAAAPEPMPPPQHPGNRRTHLLDVTAAVVGGRLRIRVGYSRAHHDRATIDRLTGDVVAR
                     LGELIDHCGRSGRTRFHTLLDKALALAPETTADVTEARPDE"
     source          1..4221
                     /culture_collection="JCM:3295"
                     /db_xref="taxon:46158"
                     /mol_type="genomic DNA"
                     /organism="Actinomadura citrea"
                     /strain="JCM 3295"
                     /submitter_seqid="sequence49"
                     /type_material="type strain of Actinomadura citrea"
     region          1..4221
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..4221
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        4..183
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41130_PCP.1"
                     /evalue="1.40E-18"
                     /label="IEZ14_RS41130_PCP.1"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="60"
                     /protein_start="0"
                     /score="58.9"
                     /tool="antismash"
                     /translation="AGLWSEVLGVSEIGVHDNFFTLGGDSITSIVVMSRAARRGLRITP
                     WQFFENQTVAGQARV"
     aSModule        4..1590
                     /domains="nrpspksdomains_IEZ14_RS41130_PCP.1"
                     /domains="nrpspksdomains_IEZ14_RS41130_Epimerization.1"
                     /domains="nrpspksdomains_IEZ14_RS41130_TIGR01720.1"
                     /incomplete
                     /locus_tags="IEZ14_RS41130"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        238..1155
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41130_Epimerization.1"
                     /evalue="1.00E-105"
                     /label="IEZ14_RS41130_Epimerization.1"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="384"
                     /protein_start="78"
                     /score="346.1"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVIDA"
                     /tool="antismash"
                     /translation="GPAPLTPVQRWFFDQAFSHPGHYNQGWLFRVPATVDADRLAAAIT
                     GLLERHDALRLRFPRDTRAAGGRRAEYTAPGAAPAPAAFRRVDLSGSAQWEPALARALA
                     DAQDIDLERDPPLRALLIDGGREDRCRLALIAHHLVIDAVSWRILLDDLREAYRALAAG
                     GEAGITDATTSFASWARALTELPGAVADDLEFWRAQRPSGRWEVPLDRQASGAGTYGDA
                     EDVEGVLGAEETTVLLRDLPAIHHSRVDEVLLTALALAMTKSFGITRLYVDVEGHGREQ
                     HLVEGADLSRTVGWFTAIAPLLLRL"
     CDS_motif       250..279
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41130_0001"
                     /evalue="2.30E-04"
                     /label="NRPS-E1"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="92"
                     /protein_start="82"
                     /score="14.0"
                     /tool="antismash"
                     /translation="LTPVQRWFFD"
     CDS_motif       298..402
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41130_0002"
                     /evalue="2.80E-09"
                     /label="C2_DCL_024-062"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="133"
                     /protein_start="98"
                     /score="29.3"
                     /tool="antismash"
                     /translation="GHYNQGWLFRVPATVDADRLAAAITGLLERHDALR"
     CDS_motif       631..696
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41130_0003"
                     /evalue="5.60E-11"
                     /label="NRPS-E2"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="231"
                     /protein_start="209"
                     /score="34.6"
                     /tool="antismash"
                     /translation="LALIAHHLVIDAVSWRILLDDL"
     CDS_motif       1099..1149
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41130_0004"
                     /evalue="2.70E-08"
                     /label="NRPS-E5"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="382"
                     /protein_start="365"
                     /score="25.8"
                     /tool="antismash"
                     /translation="DLSRTVGWFTAIAPLLL"
     aSDomain        1174..1590
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41130_TIGR01720.1"
                     /evalue="9.20E-30"
                     /label="IEZ14_RS41130_TIGR01720.1"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="529"
                     /protein_start="390"
                     /score="95.5"
                     /tool="antismash"
                     /translation="GDALAAVKEQMRAVPHGGLGHGILRHLDPVRGQALAEPPAPQVSF
                     NYLGQFDASAETRTTRPPGDHLDLAAAPEPMPPPQHPGNRRTHLLDVTAAVVGGRLRIR
                     VGYSRAHHDRATIDRLTGDVVARLGELIDHCGRSG"
     CDS_motif       1192..1254
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41130_0005"
                     /evalue="3.70E-10"
                     /label="NRPS-E6"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="417"
                     /protein_start="396"
                     /score="31.9"
                     /tool="antismash"
                     /translation="VKEQMRAVPHGGLGHGILRHL"
     CDS_motif       1294..1323
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41130_0006"
                     /evalue="2.60E-03"
                     /label="NRPS-E7"
                     /locus_tag="IEZ14_RS41130"
                     /protein_end="440"
                     /protein_start="430"
                     /score="10.3"
                     /tool="antismash"
                     /translation="PQVSFNYLGQ"
     gene            1670..>4221
                     /locus_tag="IEZ14_RS41135"
     CDS             1670..>4221
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (16-42). E-value:
                     3.1e-13. Score: 40.7. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41135_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (19-319). E-value:
                     6.2e-99. Score: 322.8. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41135_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (485-851). E-value: 1.6e-96.
                     Score: 315.3. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41135_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IEZ14_RS41135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_189269995.1"
                     /sec_met_domain="Condensation (E-value: 6.6e-57, bitscore:
                     179.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 8.6e-97, bitscore:
                     311.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTVEPAQRQRPAAADVIEDIHPLSPLQQGLLFHTLRESDSAVY
                     LSQTTALLSGLDPVAFRRSWEQTVAWHPALRSAFLWEGADEPVQVVYRRAPVRFHTQDW
                     RDLPPEVRQRRLTAFLDRDRDEPFRLSHAPLARYALLRLGEDDYQFVWTYHAILLDGWS
                     TAMVMRDLVRHGAAARAGEVMPSPDRAPYRDFVEWTRLRDHDEAHRHWRETLRGFTKPT
                     PLPVAGPGGSDRGGRTGVAEHTARLSPADTERVAAAARTYRVPLGVMVQGAWALTLMRH
                     TGVDDVVFGQVVSGRGGDLPGVQDMVGLLTNTVPVRAAAAHDDRVGTWLRALHARQSAT
                     LQYEHTPLSQVQRCSEMLAGQELFQTVLVVQNYPRLDETGQIDPGYRPSDLRGFETTHY
                     PITVFAVPGERLEIRVVHDQATVDGGSAALLAESVLTALNGLAAGADRPLGTVPVFGTG
                     TAATRGLRDRVNATERRFPPPHTVAELFEAQVGRTPDAPAVLQADSRLSYRELDRRTDR
                     LARTLVEQGARAEERVAVLLERSVEFVTAAMAILKSGAAYVPIDPGYPAARIAWMIEDG
                     DCRTVITRSDLLDRLPADCGAAVRIVCLDSPPAEGGSTGPARPLPAPARPGNPAYVIYT
                     SGSTGTPKGVVVDHRAVLNTLRWLREELGCGPGDVVAFKTPVSFTDSVCEVLWPLCHGA
                     ALAVFDDDTVRDPDALYEALDRHDVTYCQFVPRQLDLFMAAAARRGDDDPLPRLRRVFN
                     GGEPLPQSFAVEWDRQFRNGRIANIYGMTESAIYATYQALPRGFAESRGAVPIGRPIAN
                     TRVHVLNRELEPCPPGVVGELFVGGAGLARGYLGRPGLT"
     aSDomain        1718..1795
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41135_NRPS-COM_Nterm.1"
                     /evalue="3.10E-13"
                     /label="IEZ14_RS41135_NRPS-COM_Nterm.1"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="42"
                     /protein_start="16"
                     /score="40.7"
                     /tool="antismash"
                     /translation="DVIEDIHPLSPLQQGLLFHTLRESDS"
     aSDomain        1727..2626
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41135_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="6.20E-99"
                     /label="IEZ14_RS41135_Condensation_DCL.1"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="319"
                     /protein_start="19"
                     /score="322.8"
                     /specificity="C activity: inactive"
                     /specificity="Active site motif: HAILLDG"
                     /tool="antismash"
                     /translation="EDIHPLSPLQQGLLFHTLRESDSAVYLSQTTALLSGLDPVAFRRS
                     WEQTVAWHPALRSAFLWEGADEPVQVVYRRAPVRFHTQDWRDLPPEVRQRRLTAFLDRD
                     RDEPFRLSHAPLARYALLRLGEDDYQFVWTYHAILLDGWSTAMVMRDLVRHGAAARAGE
                     VMPSPDRAPYRDFVEWTRLRDHDEAHRHWRETLRGFTKPTPLPVAGPGGSDRGGRTGVA
                     EHTARLSPADTERVAAAARTYRVPLGVMVQGAWALTLMRHTGVDDVVFGQVVSGRGGDL
                     PGVQDMVGLLTNTVPVRAA"
     aSModule        1727..>4221
                     /domains="nrpspksdomains_IEZ14_RS41135_Condensation_DCL.1"
                     /domains="nrpspksdomains_IEZ14_RS41135_AMP-binding.1"
                     /incomplete
                     /locus_tags="IEZ14_RS41135"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1739..1777
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0001"
                     /evalue="1.80E-04"
                     /label="C1_DCL_004-017"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="36"
                     /protein_start="23"
                     /score="13.6"
                     /tool="antismash"
                     /translation="PLSPLQQGLLFHT"
     CDS_motif       1799..1912
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0002"
                     /evalue="1.80E-11"
                     /label="C2_DCL_024-062"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="81"
                     /protein_start="43"
                     /score="36.3"
                     /tool="antismash"
                     /translation="VYLSQTTALLSGLDPVAFRRSWEQTVAWHPALRSAFLW"
     CDS_motif       2138..2188
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0003"
                     /evalue="9.00E-06"
                     /label="C3_DCL_135-156"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="173"
                     /protein_start="156"
                     /score="18.3"
                     /tool="antismash"
                     /translation="ILLDGWSTAMVMRDLVR"
     CDS_motif       2525..2620
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0004"
                     /evalue="1.90E-16"
                     /label="C5_DCL_263-294"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="317"
                     /protein_start="285"
                     /score="52.4"
                     /tool="antismash"
                     /translation="DVVFGQVVSGRGGDLPGVQDMVGLLTNTVPVR"
     CDS_motif       2669..2791
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0005"
                     /evalue="2.10E-15"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="374"
                     /protein_start="333"
                     /score="49.1"
                     /tool="antismash"
                     /translation="HARQSATLQYEHTPLSQVQRCSEMLAGQELFQTVLVVQNYP"
     CDS_motif       2933..2971
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0006"
                     /evalue="8.00E+00"
                     /label="C3_starter_134-155"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="434"
                     /protein_start="421"
                     /score="-0.2"
                     /tool="antismash"
                     /translation="VDGGSAALLAESV"
     aSDomain        3125..>4221
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41135_AMP-binding.1"
                     /evalue="1.60E-96"
                     /label="IEZ14_RS41135_AMP-binding.1"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="851"
                     /protein_start="485"
                     /score="315.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEAQVGRTPDAPAVLQADSRLSYRELDRRTDRLARTLVEQGARAE
                     ERVAVLLERSVEFVTAAMAILKSGAAYVPIDPGYPAARIAWMIEDGDCRTVITRSDLLD
                     RLPADCGAAVRIVCLDSPPAEGGSTGPARPLPAPARPGNPAYVIYTSGSTGTPKGVVVD
                     HRAVLNTLRWLREELGCGPGDVVAFKTPVSFTDSVCEVLWPLCHGAALAVFDDDTVRDP
                     DALYEALDRHDVTYCQFVPRQLDLFMAAAARRGDDDPLPRLRRVFNGGEPLPQSFAVEW
                     DRQFRNGRIANIYGMTESAIYATYQALPRGFAESRGAVPIGRPIANTRVHVLNRELEPC
                     PPGVVGELFVGGAGLARGYLGRPGLT"
     CDS_motif       3311..3352
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0007"
                     /evalue="8.50E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="561"
                     /protein_start="547"
                     /score="18.8"
                     /tool="antismash"
                     /translation="MAILKSGAAYVPID"
     CDS_motif       3557..3616
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0008"
                     /evalue="4.30E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="649"
                     /protein_start="629"
                     /score="34.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGTPKGVVVDH"
     CDS_motif       4145..>4221
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41135_0009"
                     /evalue="1.10E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="IEZ14_RS41135"
                     /protein_end="851"
                     /protein_start="825"
                     /score="43.3"
                     /tool="antismash"
                     /translation="PPGVVGELFVGGAGLARGYLGRPGLT"
ORIGIN
        1 gcggggttgt ggagcgaggt tctgggcgtt tcggagatcg gggtccacga caacttcttc
       61 accctcggcg gagactcgat cacgagcatc gtggtgatgt cgcgggccgc ccgccgcggt
      121 ctgcgcatca ctccctggca gttcttcgag aaccagaccg tcgcggggca ggcccgggtg
      181 gccgtgccgg tggtggccgt cgaagaccgg gccggctccg cggcgccggc cgccggcccc
      241 gcaccgctga cgcccgtcca gcgctggttc ttcgaccagg cgttctccca tcccggccac
      301 tacaaccagg ggtggttgtt ccgcgtcccc gcgacggtgg acgccgatcg gctcgccgcc
      361 gcgatcacgg gcctgctgga gcggcacgac gcgctccggc tgcggttccc gcgggacacc
      421 cgggcggccg ggggccggcg ggcggagtac accgcgccgg gcgcggcccc ggcgcccgcc
      481 gcgttccgtc gcgtggacct gtccggctcg gcgcagtggg aacccgcgct cgcccgcgcc
      541 ctcgccgatg cccaggacat cgacctggaa cgggacccgc ccctgcgagc gctgctgatc
      601 gacggcggcc gggaggaccg gtgtcgcctc gcgttgatcg cccatcatct cgtcatcgac
      661 gcggtctcct ggcggatcct cctcgacgac ctgcgggagg cgtaccgggc gctcgccgcc
      721 ggcggtgaag ccgggatcac cgacgccacg acctcgttcg cgtcctgggc ccgcgcgctc
      781 accgaactgc ccggcgcggt cgccgacgac ctggagttct ggcgtgccca gcggccttcc
      841 gggcggtggg aggtgcccct ggaccgtcag gcgagcggtg ccggaacgta cggcgacgcc
      901 gaggacgtcg agggagtcct cggcgccgag gagaccacgg tcctgctgcg ggacctgccg
      961 gcgatccacc acagccgcgt cgacgaagtc ctgctcaccg cgctcgccct ggcgatgacc
     1021 aagtcgttcg gcatcactcg gctgtacgtc gacgtggaag ggcacggccg ggagcagcac
     1081 ctcgtggagg gggcggacct gtcccgcacg gtcgggtggt tcacggcgat tgctccgctg
     1141 ctgctgcgcc tgccggacgg ctcggccccg ggcgacgcgc tcgccgccgt caaggagcag
     1201 atgcgcgccg tcccgcacgg cggtctcggc cacggcatcc tccgccatct cgatccggtg
     1261 cgcggtcagg cactggccga accaccggct ccccaggtga gcttcaacta cctcggccag
     1321 ttcgacgcga gcgccgagac gcggacgaca cggccgcccg gcgatcacct ggacctggcc
     1381 gccgcccccg agccgatgcc cccgccgcag catccgggca accggcgcac gcacctgctg
     1441 gacgtcacgg ccgccgtggt cggcggccgg ctccggatac gcgtcggcta cagccgtgcc
     1501 caccatgacc gggcgaccat cgaccggctg accggcgatg tggtcgcccg gctcggcgag
     1561 ctgatcgacc actgcgggcg ctcgggcagg acgcgattcc acacgttgct cgacaaggcg
     1621 ctggccctgg cgccggagac cacggccgat gtgacggagg cgcgaccaga tgagtgacac
     1681 ggtggaaccg gcgcagcggc agcggccggc ggcggccgac gtcatcgagg acatccatcc
     1741 gctgtccccg ctgcagcagg gactgctgtt ccacacgttg cgcgagtcgg actcggcggt
     1801 ctatctcagc cagaccacgg ccctgttgtc cgggctcgat ccggtcgcct tccggcgcag
     1861 ctgggagcag acggtggcct ggcacccggc gctgcgttcg gccttcctgt gggagggcgc
     1921 ggacgagccg gtccaggtgg tctacaggag agcgccggtc cggttccaca cgcaggactg
     1981 gcgcgacctg ccccccgagg tcaggcaacg gcgcctcacc gccttcctcg accgggaccg
     2041 cgacgagccg ttccggctgt cccacgcgcc gctggcccgt tacgccctcc tgcggctcgg
     2101 cgaggacgac taccagttcg tctggaccta ccacgccatc ctgctcgacg gctggagcac
     2161 ggcgatggtg atgcgggacc tggtccgcca cggtgcggcg gcccgcgccg gcgaggtgat
     2221 gccgtcaccg gaccgggccc cgtaccggga cttcgtcgag tggacccgcc tgcgcgacca
     2281 cgacgaagcc caccgccact ggcgggagac gctgcgcggt ttcacgaagc cgacgccgct
     2341 gccggtcgcc gggccgggcg gctcggaccg cggcggccgg acgggggtgg ccgagcacac
     2401 cgcacggctc tcgcccgcgg acacggaacg cgtcgccgcg gccgcccgga cgtaccgggt
     2461 tccgctcggc gtcatggtgc agggcgcatg ggcgctgacg ctgatgcggc acaccggggt
     2521 cgacgacgtc gtcttcggcc aggtcgtctc cggccgcggc ggtgaccttc ccggagtgca
     2581 ggacatggtg ggacttctca ccaacaccgt cccggtccgc gcggcggccg cgcacgacga
     2641 ccgggtgggg acgtggctgc gggcgctcca cgcgcggcag tccgccaccc tgcagtacga
     2701 gcacaccccg ctgtcccagg tgcagcggtg cagcgagatg ctcgccgggc aggagctctt
     2761 ccagaccgtg ctggtcgtcc agaactatcc gcggctggac gagaccgggc agatcgaccc
     2821 cggataccgt cccagcgacc tgcgcgggtt cgagaccacc cattacccga tcacggtgtt
     2881 cgcggtgccc ggcgagcggc ttgagatacg ggtggtgcac gatcaggcga cggtcgacgg
     2941 cggaagtgcc gcgctcctcg ccgagtccgt gctcacggcg ctgaacggcc tggccgccgg
     3001 cgcggatcgg cccctcggca cggtcccggt cttcggcacg ggaacggcgg cgactcgcgg
     3061 cctgcgcgac cgggtcaacg ccaccgagcg ccggttccca cccccgcaca ccgtcgcgga
     3121 gctcttcgag gcgcaggtcg gccggacgcc cgacgcgccg gccgtcctcc aagccgattc
     3181 ccggctgagc taccgggagc tggaccggcg gaccgaccgg ctggcgcgca cgctcgtcga
     3241 gcagggcgcg cgggccgagg aacgggtggc ggtcctgctg gagcgttccg tggaattcgt
     3301 gacggcggcc atggcgattc tgaagtccgg ggccgcctac gtcccgatcg atcccgggta
     3361 tccggcggcg cgcatcgcct ggatgatcga ggacggcgac tgccgcaccg tgatcacccg
     3421 tagtgacctt ctcgaccggc tcccggccga ctgcggagcc gcggtccgga tcgtctgcct
     3481 ggactcgcct ccggccgagg gcggctcgac cgggcccgcg cgcccgctgc cggcgccggc
     3541 gcgccccggc aaccccgcct acgtgatcta cacgtccggt tccaccggaa cgccgaaggg
     3601 cgtggtggtc gatcaccggg cggtgctgaa cacgctgcgg tggctgcggg aggaactcgg
     3661 ctgcggtccc ggcgacgtgg tcgccttcaa gacccccgtc agcttcaccg actccgtctg
     3721 cgaggtgctg tggccgctct gccacggagc cgcactggcc gtcttcgacg acgacaccgt
     3781 acgcgacccg gacgcgctgt acgaggcact ggaccggcac gacgtcacct actgccaatt
     3841 cgtgccgcgc caactcgatc tgttcatggc cgccgcggcg cggcgcggcg acgatgatcc
     3901 cctgccacgc ctgcgcaggg tcttcaacgg cggtgagcca ttgccgcagt ccttcgccgt
     3961 cgaatgggac cggcagttcc gcaacggcag gatcgccaac atctacggca tgaccgagtc
     4021 ggcgatctat gcgacctacc aggctctgcc gcgggggttc gccgagagcc gaggggccgt
     4081 ccccatcggc cgtccgatcg ccaacacgcg agtgcacgtg ctcaatcgcg aactggagcc
     4141 atgcccgcct ggtgtggtgg gggagttgtt cgtgggtggt gctggtttgg cgcgtgggta
     4201 tttggggcgg ccgggtttga c
//
