LOCUS       NZ_BMRD01000047         6091 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence47, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000047
VERSION     NZ_BMRD01000047.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6091
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000047.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1524
                     /locus_tag="IEZ14_RS41110"
     CDS             <1..1524
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (36-342). E-value:
                     1.1e-124. Score: 408.8. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41110_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (347-499). E-value: 8.2e-37.
                     Score: 118.4. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41110_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852241.1"
                     /locus_tag="IEZ14_RS41110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_229810784.1"
                     /sec_met_domain="Condensation (E-value: 1.4e-40, bitscore:
                     126.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ITPREVFQHQTVEELAATATPVGEGEEIEAEAPGAGVGPVPITPI
                     IAWLRDRADGDASLISGFHQAMLLRTPPGLGTERLTAALATLLDHHDVLRLRLDADGGR
                     WQPVVRPPGSADAAALLTRVDVAGLDGDKVQAVVAEQAAAARDRLDPAAGTVAQLVWFD
                     ADREQGRLLVVLHHLVVDGVTWRILLPDLVTAWAGGELQPVGTSFRRWAQKLTAAERGG
                     QDLEDWLDIVDGPPDGLADRPLDPRADIAARARSLTLDLPADVTGPLLTDVPAAFHGRA
                     NDVLLTGLAVAVAQWRRRRGGRGTGVLVDLEGHGREDSVPGVDVSRTAGWFTSIHPVRL
                     DAGGATGGAAVKKVKEQLRAVPDVLGYGLLRHVDGDEELAEVPPAQIAFNYLGRVADGG
                     DGGDWTLAPEELPAGEDPRMPMAHALEVNALTRDLPAGPVLSATWTWPGGLLDSADVRE
                     LAEGWFAALRGLVADVAGGAAGGFTPSDLLVDLDQGEIDKLQTAWRQKK"
     source          1..6091
                     /culture_collection="JCM:3295"
                     /db_xref="taxon:46158"
                     /mol_type="genomic DNA"
                     /organism="Actinomadura citrea"
                     /strain="JCM 3295"
                     /submitter_seqid="sequence47"
                     /type_material="type strain of Actinomadura citrea"
     region          1..6091
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6091
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6091
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1505:>6091](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1506..>6091
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        112..1029
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41110_Epimerization.1"
                     /evalue="1.10E-124"
                     /label="IEZ14_RS41110_Epimerization.1"
                     /locus_tag="IEZ14_RS41110"
                     /protein_end="342"
                     /protein_start="36"
                     /score="408.8"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="VGPVPITPIIAWLRDRADGDASLISGFHQAMLLRTPPGLGTERLT
                     AALATLLDHHDVLRLRLDADGGRWQPVVRPPGSADAAALLTRVDVAGLDGDKVQAVVAE
                     QAAAARDRLDPAAGTVAQLVWFDADREQGRLLVVLHHLVVDGVTWRILLPDLVTAWAGG
                     ELQPVGTSFRRWAQKLTAAERGGQDLEDWLDIVDGPPDGLADRPLDPRADIAARARSLT
                     LDLPADVTGPLLTDVPAAFHGRANDVLLTGLAVAVAQWRRRRGGRGTGVLVDLEGHGRE
                     DSVPGVDVSRTAGWFTSIHPVRLDA"
     aSModule        112..1500
                     /domains="nrpspksdomains_IEZ14_RS41110_Epimerization.1"
                     /domains="nrpspksdomains_IEZ14_RS41110_TIGR01720.1"
                     /incomplete
                     /locus_tags="IEZ14_RS41110"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       514..579
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41110_0001"
                     /evalue="1.70E-10"
                     /label="NRPS-E2"
                     /locus_tag="IEZ14_RS41110"
                     /protein_end="192"
                     /protein_start="170"
                     /score="33.1"
                     /tool="antismash"
                     /translation="LLVVLHHLVVDGVTWRILLPDL"
     CDS_motif       973..1023
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41110_0002"
                     /evalue="2.10E-08"
                     /label="NRPS-E5"
                     /locus_tag="IEZ14_RS41110"
                     /protein_end="340"
                     /protein_start="323"
                     /score="26.2"
                     /tool="antismash"
                     /translation="DVSRTAGWFTSIHPVRL"
     aSDomain        1045..1500
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41110_TIGR01720.1"
                     /evalue="8.20E-37"
                     /label="IEZ14_RS41110_TIGR01720.1"
                     /locus_tag="IEZ14_RS41110"
                     /protein_end="499"
                     /protein_start="347"
                     /score="118.4"
                     /tool="antismash"
                     /translation="GAAVKKVKEQLRAVPDVLGYGLLRHVDGDEELAEVPPAQIAFNYL
                     GRVADGGDGGDWTLAPEELPAGEDPRMPMAHALEVNALTRDLPAGPVLSATWTWPGGLL
                     DSADVRELAEGWFAALRGLVADVAGGAAGGFTPSDLLVDLDQGEIDKL"
     CDS_motif       1063..1119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41110_0003"
                     /evalue="7.50E-07"
                     /label="NRPS-E6"
                     /locus_tag="IEZ14_RS41110"
                     /protein_end="372"
                     /protein_start="353"
                     /score="21.6"
                     /tool="antismash"
                     /translation="VKEQLRAVPDVLGYGLLRH"
     gene            1506..>6091
                     /locus_tag="IEZ14_RS41115"
     CDS             1506..>6091
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (3-31). E-value: 2.1e-09.
                     Score: 28.4. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41115_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (11-301). E-value:
                     3.7e-113. Score: 369.5. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41115_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (461-852). E-value:
                     2.2e-105. Score: 344.5. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41115_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (953-1022). E-value: 9e-28. Score:
                     88.3. Matches aSDomain: nrpspksdomains_IEZ14_RS41115_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1036-1337). E-value:
                     1.2e-117. Score: 384.1. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41115_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852241.1"
                     /locus_tag="IEZ14_RS41115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_229810785.1"
                     /sec_met_domain="AMP-binding (E-value: 6.1e-108, bitscore:
                     348.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.1e-19, bitscore:
                     57.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.5e-71, bitscore:
                     226.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAEEVNRGDLEDILPLSPLQQGFFFHALFDAGDGDVYTAQFVLD
                     LEGPLDVAALRTAAATLLRRHPNLRAAFWHEDLSRPVQVVPRTVELPWEEVTAAGAEAD
                     AVVLRERARGFDMTAPPLLRFVLVRTGPESHRLIFTNHHILLDGWSTPIMATELFLLYM
                     QGGHDAGMPRVTPYKNYLAWLSQQDRDAAEDAWRRALDGVGEPSLVAPAAAARAPEPPE
                     RTITGLDERTTRRLTRAARRHGVTLSTVLQGAWGLVLGRFLGTGDVVFGATVSGRPPEL
                     PGVEQMIGLFINTLPVRVRYRLDEPLGALMERLQDEQTELLAHHHLGLTDIQRAAGHGG
                     ALFDTMTVLENYPFDPSSMDGPLNGVRIAAADSYDATHFPLSFVAVPGESLSLRLHYRP
                     DVFEAPTVEALLARVRRFLEAFAEDADQVIGAVDLAGDADRAALAAWNDTAADVPSGTL
                     PALFEAQAARTPDDTALVCGEARLTYADLNARANRLAHELIARGVGPEDRVALVLPRTP
                     EIVVAILGVLKAGAAYVPVDPEYPADRIASTLDDCRPALTITAGDFPDAAGRPSHDPSD
                     GERIRSLRADNAAYVIYTSGSTGRPKGVTVPHVAVLNYFEAHRDAFFDPAIEAAGGRRM
                     RFAHLASFSFDTSWMGLLWMTYGHELHLIDDETRRDVEAYTAYVARARIDLVNTTPSHF
                     TSLREAGLLDADGRHLPSQLLLGGEAISENLWTELRAVPGLTTRNFYGPTESTIDTMNA
                     VVARSGRPTVGAPYRNTRTYVLDRALQPVPAGVPGELYLAGVQLARGYHDRPGLSAERF
                     VADPFGAPGERMYRTGDLGRWRPDGTVEYLGRADDQVKIRGFRVEPGEIENVLAGCPGV
                     ARAAVVVREDRPGDRRLAAYLVGPDVDAAAVRRYAAERLPDHMVPSFTVLEALPLNVNG
                     KLDRAALPAPDAGAAGRAPRSPQEEILCGLFAEILGVPRAGVDDGFFDLGGDSLTATRL
                     VSRIRSALGAELPVRALFEAPTVAGLAERLAEASGDVRPPLVRAQRPDVVPLSYAQQRL
                     WFLNRFEGAPATFNMPIALRLDGALDLAALRAALADVVARHEPLRTIFPDRSGTARQLV
                     LGAADARPRLDVAEVEESALPAALAATVGQGFDLSVEPPLRARLFALGGGTHVLMLVLH
                     HIAADGWSMAPLARDVILAYAARAEGREPEWAPLPVQYADYTLWQRELFGSEDDPGSLI
                     SQQIAYWREALAGLPEELPLPADRPRPAEASYRGGAHGFELDAELHAELLALARQSGAS
                     LFMVMQAAFAALLTRLGAGTDVPIGSPIAGRTDEALDDLVGMFVNMLVLRTDTSGDPSF
                     RELVGRVKETDLAAYAHQDLPFERLVEVLNPPRSMARHPLFQVVLSFQNNPEARLELDG
                     LTGGAEPLAAGAAKYDLSLYLEERHGGDGAPAGIEAGLEYALDLFDPATAQAIARRFER
                     LVRELVADPDAPITTAEILDRGERRTLLRRWAGGKGGDVPRTTIPALFEEQAAVRPGAV
                     AVSFEGVS"
     aSDomain        1515..1598
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41115_NRPS-COM_Nterm.1"
                     /evalue="2.10E-09"
                     /label="IEZ14_RS41115_NRPS-COM_Nterm.1"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="31"
                     /protein_start="3"
                     /score="28.4"
                     /tool="antismash"
                     /translation="EEVNRGDLEDILPLSPLQQGFFFHALFD"
     aSDomain        1539..2408
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41115_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.70E-113"
                     /label="IEZ14_RS41115_Condensation_DCL.1"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="301"
                     /protein_start="11"
                     /score="369.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILLDG"
                     /tool="antismash"
                     /translation="EDILPLSPLQQGFFFHALFDAGDGDVYTAQFVLDLEGPLDVAALR
                     TAAATLLRRHPNLRAAFWHEDLSRPVQVVPRTVELPWEEVTAAGAEADAVVLRERARGF
                     DMTAPPLLRFVLVRTGPESHRLIFTNHHILLDGWSTPIMATELFLLYMQGGHDAGMPRV
                     TPYKNYLAWLSQQDRDAAEDAWRRALDGVGEPSLVAPAAAARAPEPPERTITGLDERTT
                     RRLTRAARRHGVTLSTVLQGAWGLVLGRFLGTGDVVFGATVSGRPPELPGVEQMIGLFI
                     NTLPVRVRY"
     aSModule        1539..4571
                     /complete
                     /domains="nrpspksdomains_IEZ14_RS41115_Condensation_DCL.1"
                     /domains="nrpspksdomains_IEZ14_RS41115_AMP-binding.1"
                     /domains="nrpspksdomains_IEZ14_RS41115_PCP.1"
                     /locus_tags="IEZ14_RS41115"
                     /monomer_pairings="X -> X"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1551..1589
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0001"
                     /evalue="4.50E-03"
                     /label="C1_DCL_004-017"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="28"
                     /protein_start="15"
                     /score="9.7"
                     /tool="antismash"
                     /translation="PLSPLQQGFFFHA"
     CDS_motif       1611..1724
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0002"
                     /evalue="4.20E-16"
                     /label="C2_DCL_024-062"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="73"
                     /protein_start="35"
                     /score="51.2"
                     /tool="antismash"
                     /translation="DVYTAQFVLDLEGPLDVAALRTAAATLLRRHPNLRAAF"
     CDS_motif       1926..1991
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0003"
                     /evalue="7.50E-10"
                     /label="C3_DCL_135-156"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="162"
                     /protein_start="140"
                     /score="31.3"
                     /tool="antismash"
                     /translation="NHHILLDGWSTPIMATELFLLY"
     CDS_motif       2031..2069
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0004"
                     /evalue="4.30E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="188"
                     /protein_start="175"
                     /score="9.9"
                     /tool="antismash"
                     /translation="PYKNYLAWLSQQD"
     CDS_motif       2304..2399
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0005"
                     /evalue="1.00E-22"
                     /label="C5_DCL_263-294"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="298"
                     /protein_start="266"
                     /score="72.5"
                     /tool="antismash"
                     /translation="DVVFGATVSGRPPELPGVEQMIGLFINTLPVR"
     CDS_motif       2448..2564
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0006"
                     /evalue="1.10E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="353"
                     /protein_start="314"
                     /score="56.4"
                     /tool="antismash"
                     /translation="QDEQTELLAHHHLGLTDIQRAAGHGGALFDTMTVLENYP"
     aSDomain        2889..4061
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41115_AMP-binding.1"
                     /evalue="2.20E-105"
                     /label="IEZ14_RS41115_AMP-binding.1"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="852"
                     /protein_start="461"
                     /score="344.5"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEAQAARTPDDTALVCGEARLTYADLNARANRLAHELIARGVGPE
                     DRVALVLPRTPEIVVAILGVLKAGAAYVPVDPEYPADRIASTLDDCRPALTITAGDFPD
                     AAGRPSHDPSDGERIRSLRADNAAYVIYTSGSTGRPKGVTVPHVAVLNYFEAHRDAFFD
                     PAIEAAGGRRMRFAHLASFSFDTSWMGLLWMTYGHELHLIDDETRRDVEAYTAYVARAR
                     IDLVNTTPSHFTSLREAGLLDADGRHLPSQLLLGGEAISENLWTELRAVPGLTTRNFYG
                     PTESTIDTMNAVVARSGRPTVGAPYRNTRTYVLDRALQPVPAGVPGELYLAGVQLARGY
                     HDRPGLSAERFVADPFGAPGERMYRTGDLGRWRPDGTVEYLGRADDQVKIR"
     CDS_motif       3075..3116
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0007"
                     /evalue="9.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="537"
                     /protein_start="523"
                     /score="18.7"
                     /tool="antismash"
                     /translation="LGVLKAGAAYVPVD"
     CDS_motif       3270..3329
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0008"
                     /evalue="4.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="608"
                     /protein_start="588"
                     /score="31.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVTVPH"
     CDS_motif       3852..3941
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0009"
                     /evalue="1.10E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="812"
                     /protein_start="782"
                     /score="49.7"
                     /tool="antismash"
                     /translation="PAGVPGELYLAGVQLARGYHDRPGLSAERF"
     CDS_motif       4026..4091
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0010"
                     /evalue="4.10E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="862"
                     /protein_start="840"
                     /score="41.1"
                     /tool="antismash"
                     /translation="YLGRADDQVKIRGFRVEPGEIE"
     aSDomain        4365..4571
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41115_PCP.1"
                     /evalue="9.00E-28"
                     /label="IEZ14_RS41115_PCP.1"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1022"
                     /protein_start="953"
                     /score="88.3"
                     /tool="antismash"
                     /translation="EEILCGLFAEILGVPRAGVDDGFFDLGGDSLTATRLVSRIRSALG
                     AELPVRALFEAPTVAGLAERLAEA"
     aSDomain        4614..5516
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41115_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.20E-117"
                     /label="IEZ14_RS41115_Condensation_LCL.1"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1337"
                     /protein_start="1036"
                     /score="384.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAADG"
                     /tool="antismash"
                     /translation="DVVPLSYAQQRLWFLNRFEGAPATFNMPIALRLDGALDLAALRAA
                     LADVVARHEPLRTIFPDRSGTARQLVLGAADARPRLDVAEVEESALPAALAATVGQGFD
                     LSVEPPLRARLFALGGGTHVLMLVLHHIAADGWSMAPLARDVILAYAARAEGREPEWAP
                     LPVQYADYTLWQRELFGSEDDPGSLISQQIAYWREALAGLPEELPLPADRPRPAEASYR
                     GGAHGFELDAELHAELLALARQSGASLFMVMQAAFAALLTRLGAGTDVPIGSPIAGRTD
                     EALDDLVGMFVNMLVLRTDT"
     CDS_motif       4629..4661
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0011"
                     /evalue="3.10E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1052"
                     /protein_start="1041"
                     /score="10.1"
                     /tool="antismash"
                     /translation="SYAQQRLWFLN"
     CDS_motif       4680..4796
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0012"
                     /evalue="1.70E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1097"
                     /protein_start="1058"
                     /score="59.0"
                     /tool="antismash"
                     /translation="ATFNMPIALRLDGALDLAALRAALADVVARHEPLRTIFP"
     CDS_motif       4998..5063
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0013"
                     /evalue="3.40E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1186"
                     /protein_start="1164"
                     /score="29.2"
                     /tool="antismash"
                     /translation="LHHIAADGWSMAPLARDVILAY"
     CDS_motif       5112..5147
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0014"
                     /evalue="3.80E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1214"
                     /protein_start="1202"
                     /score="16.2"
                     /tool="antismash"
                     /translation="QYADYTLWQREL"
     CDS_motif       5418..5507
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0015"
                     /evalue="1.10E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1334"
                     /protein_start="1304"
                     /score="46.7"
                     /tool="antismash"
                     /translation="DVPIGSPIAGRTDEALDDLVGMFVNMLVLR"
     CDS_motif       5556..5675
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEZ14_RS41115_0016"
                     /evalue="2.20E-22"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IEZ14_RS41115"
                     /protein_end="1390"
                     /protein_start="1350"
                     /score="71.5"
                     /tool="antismash"
                     /translation="KETDLAAYAHQDLPFERLVEVLNPPRSMARHPLFQVVLSF"
ORIGIN
        1 atcactcccc gcgaggtctt ccagcaccag accgtcgaag aactcgccgc caccgccaca
       61 ccggtcggcg aaggcgagga gatcgaagcg gaggcgccgg gtgccggggt cggccccgtc
      121 cccatcaccc cgatcatcgc ctggctgcgc gaccgggcgg acggcgacgc gtcgctcatc
      181 agcggcttcc accaggcgat gctgctgcgc accccgcccg gcctcggcac ggagcggctg
      241 accgccgcgc tggcgaccct gctcgaccac cacgacgtcc tgcgcctccg cctggacgcc
      301 gacggcggcc ggtggcagcc ggtcgtccgc ccgccgggtt cggccgacgc cgccgccctc
      361 ctcacccgcg tcgacgtcgc gggcctggac ggcgacaagg tccaggccgt cgtcgccgag
      421 caggccgccg ccgcccgcga ccgcctcgac ccggccgccg ggacggtcgc gcagctcgtc
      481 tggttcgacg cggaccgcga gcaggggcgc ctcctcgtcg tcctgcacca cctggtcgtg
      541 gacggcgtga cctggcgcat cctgctcccc gacctcgtca ccgcctgggc cgggggagag
      601 ctccagccgg tcgggacgtc cttccgccgc tgggcgcaga agctcaccgc cgccgagcgg
      661 ggcgggcagg acctggagga ctggctcgac atcgtcgacg gcccgccgga cgggctcgct
      721 gaccgaccgc tcgacccgcg cgcggacatc gcggcccgcg cccgctcgct caccctcgac
      781 ctgcccgccg acgtcacggg gccgctgctc accgacgtgc ccgccgcgtt ccacgggcgc
      841 gccaacgacg tgctgctcac cggcctcgcc gtcgccgtcg cccagtggcg caggcgccgc
      901 ggcgggcgcg gcaccggcgt gctggtcgac ctggagggcc acggccgcga ggactccgtg
      961 ccgggcgtgg acgtctcgcg cacggccggc tggttcacct cgatccaccc cgtccggctg
     1021 gacgcgggcg gcgcgaccgg cggcgcggcc gtcaagaagg tgaaggagca gctgcgcgcc
     1081 gtcccggacg tcctcggcta cgggctgctg cgccacgtgg acggggacga ggagctggcc
     1141 gaggtcccgc ccgcgcagat cgcgttcaac tacctcggcc gggtcgcgga cggcggcgac
     1201 ggaggcgact ggacgctcgc cccggaggag ctgccggccg gggaggaccc ccgcatgccg
     1261 atggcgcacg cgctggaggt caacgccctc acccgggacc tgcccgcggg cccggtgctg
     1321 tcggcgacct ggacgtggcc cggcgggctg ctggactccg ccgacgtccg ggagctggcc
     1381 gagggctggt tcgcggcgct gcgcggcctc gtcgcggacg tcgccggcgg cgcggccggc
     1441 ggattcaccc cgtccgacct gctggtggac ctggaccagg gcgagatcga caagctgcag
     1501 accgcgtgga ggcagaagaa gtgaaccggg gcgacctcga ggacatcctg ccgctgtcgc
     1561 cgctgcagca gggcttcttc ttccacgccc tcttcgacgc cggcgacggc gacgtctaca
     1621 cggcccagtt cgtcctggac ctggagggcc ccctcgacgt cgccgcgctg cggaccgccg
     1681 ccgcgacgct gctgcgccgc caccccaacc tgcgcgccgc gttctggcac gaggacctgt
     1741 cgcggcccgt gcaggtcgtg ccgcgcaccg tcgagctgcc gtgggaggag gtcaccgccg
     1801 ccggcgccga ggccgacgcc gtcgtgctgc gggaacgggc gcgcggcttc gacatgaccg
     1861 cgccgccgct gctgcggttc gtcctcgtcc gcacgggccc tgagagccac cgcctgatct
     1921 tcaccaacca ccacatcctg ctggacggct ggtccacgcc gatcatggcg accgagctgt
     1981 tcctgctgta catgcagggc ggccacgacg cggggatgcc gcgcgtcacc ccctacaaga
     2041 actacctggc ctggctctcc caacaggacc gcgacgccgc cgaggacgcc tggcgccgcg
     2101 ccctggacgg cgtcggggag cccagcctcg tcgcccccgc ggccgccgcc cgcgcccccg
     2161 aaccccccga gcgcacgatc accgggctgg acgagcgcac cacccgccgc ctcacccgcg
     2221 ccgcgcgccg ccacggcgtc acgctgagca ccgtcctgca gggcgcctgg ggactcgtcc
     2281 tcggacggtt cctcggcacg ggcgacgtgg tgttcggcgc gaccgtgtcc ggccgtcccc
     2341 ccgagctgcc gggcgtcgag cagatgatcg ggctgttcat caacacgctg ccggtccgcg
     2401 tccggtaccg gctggacgag ccgctcggcg cgctgatgga gcggctgcag gacgagcaga
     2461 ccgaactcct cgcccatcac catctcggcc tcaccgacat ccagcgggcg gcgggccacg
     2521 gcggcgcgct gttcgacacg atgacggtgc tggagaacta cccgttcgac ccgtcctcga
     2581 tggacggccc cctcaacggc gtccgcatcg ccgcggccga ctcctacgac gccacccact
     2641 tccccctgtc gttcgtcgcc gtccccggcg agagcctctc gctccgcctg cactaccggc
     2701 ccgacgtctt cgaggcgccc accgtcgagg cgctgctcgc ccgcgtccgc cgcttcctgg
     2761 aggcgttcgc cgaggacgcg gaccaggtga tcggcgcggt ggacctcgcc ggcgacgccg
     2821 atcgggcggc cctcgccgcc tggaacgaca ccgccgccga cgtcccgtcc ggcaccctcc
     2881 cggccctgtt cgaggcgcag gccgcccgca ccccggacga caccgccctc gtctgcggcg
     2941 aggcccggct gacctacgcc gacctgaacg cgcgcgccaa ccgcctcgcc cacgagctga
     3001 tcgcccgggg cgtcggcccc gaggaccgcg tcgcgctcgt cctgccgcgc actcccgaga
     3061 tcgtcgtcgc gatcctcggc gtcctgaagg ccggcgccgc gtacgtgccg gtcgaccccg
     3121 agtacccggc cgatcgcatc gcctccacgc tcgacgactg ccgcccggcg ctgaccatca
     3181 ccgccggcga cttcccggac gccgccggac ggccctccca cgacccctcc gacggcgagc
     3241 ggatccggag cctccgggcg gacaacgccg cctacgtcat ctacacgtcg ggatccaccg
     3301 ggcgccccaa gggcgtgacc gtcccgcacg tggccgtgct caactacttc gaggcgcacc
     3361 gggacgcgtt cttcgaccct gcgatcgagg cagccggtgg ccgccggatg cggttcgccc
     3421 acctggcgtc gttctcgttc gacacgtcct ggatgggcct gctgtggatg acgtacggcc
     3481 acgagctgca cctcatcgac gacgagaccc gccgcgacgt ggaggcctac accgcgtacg
     3541 tggcgcgcgc gcggatcgac ctggtgaaca ccaccccgtc gcacttcacc tcgctccgcg
     3601 aggccggcct cctggacgcc gacggccggc acctgccctc gcagctcctc ctcggcggcg
     3661 aggcgatcag cgagaacctg tggacggagc tgcgcgcggt ccccgggctc accacgcgca
     3721 acttctacgg gcccaccgag tcgaccatcg acaccatgaa cgcggtggtc gcccgcagcg
     3781 ggcgcccgac cgtcggcgcc ccctaccgga acacccggac ctacgtgctg gaccgcgcgc
     3841 tccagcccgt cccggccggc gtccccggcg agctgtacct cgcgggcgtg cagctcgcgc
     3901 gcggctacca cgaccggccc ggcctgtccg ccgagcggtt cgtcgccgac ccgttcggcg
     3961 cgcccggcga gcgcatgtac cgcaccggtg acctcggccg gtggcggccg gacgggacgg
     4021 tggagtacct cggccgcgcc gacgaccagg tcaagatccg cggcttccgg gtggagccag
     4081 gcgagatcga gaacgttctc gccggatgcc cgggcgtcgc gcgggccgcc gtcgtcgtcc
     4141 gcgaggaccg gccgggcgac cggcgcctcg ccgcctacct cgtcgggccg gacgtcgacg
     4201 ccgccgccgt ccggcggtac gcggccgagc ggctgcccga ccacatggtc ccctcgttca
     4261 ccgtcctgga ggcgctgccg ctcaacgtga acggcaagct cgaccgggcc gcgctgcccg
     4321 cccccgacgc cggcgccgcc ggccgcgcgc cgcgctcgcc gcaggaggag atcctctgcg
     4381 ggctgttcgc cgagatcctc ggcgtgccga gggccggcgt cgacgacggc ttcttcgacc
     4441 tcggcggcga ctccctcacc gcgacccgcc tcgtcagccg gatccggtcc gcgctcggcg
     4501 ccgagctgcc cgtccgggcg ctgttcgagg cgccgaccgt cgcgggcctc gccgagcggc
     4561 tcgccgaggc ctccggtgac gtccgcccgc cgctggtccg ggcccagcgt ccggacgtcg
     4621 tcccgctgtc gtacgcgcag cagcggctct ggttcctcaa ccggttcgag ggcgcccccg
     4681 cgacgttcaa catgccgatc gcgctgcgcc tggacggcgc cctcgacctc gccgcgctgc
     4741 gcgccgcgct ggccgacgtc gtggcgcgcc acgagccgct tcgcacgatc ttccccgacc
     4801 ggtccggaac cgcccgccag ctcgtcctgg gcgcggccga cgcccgcccc cgcctcgacg
     4861 tcgcggaggt ggaggagagc gcgctgccgg cggcgctggc cgcgacggtc ggacagggct
     4921 tcgacctgtc ggtggagccg ccgctgcggg cccgcctgtt cgcgctcggc ggcggcacgc
     4981 acgtcctcat gctcgtcctg caccacatcg cggcggacgg ctggtcgatg gcgccgctcg
     5041 cccgcgacgt gatcctcgcc tacgcggccc gagcggaggg ccgcgagccg gagtgggcgc
     5101 cgctgcccgt ccagtacgcc gactacacgc tctggcagcg cgagctgttc ggttccgagg
     5161 acgatcccgg cagcctgatc tcgcagcaga tcgcgtactg gcgcgaggcg ctggcggggc
     5221 tgccggagga gctgccgctg cccgccgacc ggccgcgccc ggcggaggcg tcctaccggg
     5281 gcggggcgca cgggttcgag ctggacgccg aactccacgc cgagctcctg gccctcgccc
     5341 ggcagagcgg cgcgagcctc ttcatggtca tgcaggccgc gttcgcggcg ctgctcacgc
     5401 ggctcggcgc ggggacggac gtgccgatcg gctcgccgat cgcgggccgg accgacgagg
     5461 ctctggacga tctggtcggc atgttcgtca acatgctcgt cctgcgcacc gacacctcag
     5521 gggacccgtc gttccgggag ctggtgggac gggtgaagga gaccgacctc gccgcctacg
     5581 cgcaccagga cctgccgttc gagcggctcg tcgaggtgct caacccgccc cggtcgatgg
     5641 cgcggcaccc gctgttccag gtcgtcctgt cgttccagaa caaccccgag gcgcggctcg
     5701 aactggacgg cctgaccggc ggcgccgagc ccctggcggc gggcgccgcc aagtacgacc
     5761 tctccctcta cctggaggag cggcacggcg gcgacggcgc gcccgccggc atcgaggccg
     5821 gactcgaata cgccctcgac ctcttcgacc ccgccaccgc ccaggcgatc gcccgccggt
     5881 tcgagcgcct cgtccgcgaa ctcgtcgccg accccgacgc gcccatcacc accgccgaga
     5941 tcctcgaccg cggcgaacgg cgcaccctgc tgcgccgctg ggcgggcgga aagggcggcg
     6001 acgtcccgcg cacgacgatc cccgcgctgt tcgaggagca ggcggcggtt cgtccgggtg
     6061 cggtggcggt gtcgttcgag ggtgtgtcgt g
//
