LOCUS       NZ_BMRD01000046         7432 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence46, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000046
VERSION     NZ_BMRD01000046.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7432
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..139
                     /locus_tag="IEZ14_RS43020"
     source          1..7432
                     /culture_collection="JCM:3295"
                     /db_xref="taxon:46158"
                     /mol_type="genomic DNA"
                     /organism="Actinomadura citrea"
                     /strain="JCM 3295"
                     /submitter_seqid="sequence46"
                     /type_material="type strain of Actinomadura citrea"
     region          1..7432
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="PKS-like"
                     /region_number="1"
                     /rules="(t2pks2 or ksIII)"
                     /tool="antismash"
     cand_cluster    1..7432
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2pks2 or ksIII)"
                     /kind="single"
                     /product="PKS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7432
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3223:4273](-)"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3224..4273)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
     CDS             <3..139
                     /codon_start=2
                     /inference="COORDINATES: protein motif:HMM:NF037656.5"
                     /locus_tag="IEZ14_RS43020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_189269972.1"
                     /transl_table=11
                     /translation="GNGREVDKLVRSAMTAHARRTEQLASTGAQLTTEQLATLLPEDIT
                     "
     gene            complement(136..1797)
                     /locus_tag="IEZ14_RS41075"
                     /old_locus_tag="GCM10010177_82950"
     CDS             complement(136..1797)
                     /EC_number="3.2.1.-"
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Alpha-amylase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1257: Alpha-glucosidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893359.1"
                     /locus_tag="IEZ14_RS41075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_82950"
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="WP_179837918.1"
                     /sec_met_domain="Alpha-amylase (E-value: 3.3e-73, bitscore:
                     236.1, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLSSTSPSPTRGVRRPAESSSPLVGDTRWWRDAVIYQIYVRSFA
                     DADGDGIGDLAGVRDRLPYLASLGVDALWITPFYESPMVDGGYDVTNYRSVDPTFGTLA
                     DAADLIEDAHGLGLRVIVDIVPNHTSDQHVWFQEALAAGISGPARERYVFRPGRGAAGE
                     LPPNDWESVFGGPAWTRLPDGQWYLHLFAPQQPDLNWEHPDVRAEFDDVLRFWLDLAVD
                     GFRIDVAHGMVKAAGLPDVGDAPQSRLLGRGKVPYFDQDGVHEIHRGWRRLLDSYPGER
                     IGVAEAWAPSLDRLANYVRPDELHQVFNFDYMRTDWDAGALRSVIEKSLASAGSVGAPT
                     TWVLSNHDVQRHVTRYGDGDLGQRRARAAALLTLALPGSAYVYQGEELGLPEVLDIPAE
                     LLTDPQLESIKGGRDGCRVPLPWSGYEPPFGFGSGVGWLPSPAEWKSLTVEAQRDDDAS
                     MLTLYRQALRIRRDHPALGDGELRWLDEPPGCLVFERTGHSGGPRMLCAVNTGDQSIRF
                     SAPGSLLLSSGPIRTAGRDAILPPDTAAWWSVTPED"
     gene            complement(1878..3173)
                     /locus_tag="IEZ14_RS41080"
                     /old_locus_tag="GCM10010177_82960"
     CDS             complement(1878..3173)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851795.1"
                     /locus_tag="IEZ14_RS41080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_82960"
                     /product="cytochrome P450"
                     /protein_id="WP_229810666.1"
                     /sec_met_domain="p450 (E-value: 4.7e-15, bitscore: 44.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHLDAQTSDRQQSAPIVTIDPLGADPHAEMARLRAAGPVVRTVL
                     PGLPDTVHAWMVTRQDLLSRLSRHPAVSRVAHRHWAAYRDGLVPADWPLAGMFIGLTNM
                     FFLDDPEHQHHRRMISSVFTAKRVEQLSADITRIVRHALDALPGHRGPDGAVDLRAHYA
                     YTVPMGVICHLLGIPESMRPQFRNLVDTLLRTDAKTAQDAAALEQGRLDLLDELVELRL
                     ARPGADLTSSLITVRDNGGGALLHRQLLDTLWSLVIAGHETTLNLITNAVRALLTHPDQ
                     LAHARALPPDGWSAVVEETLRWDGVINYLVAGYTTDTIPIGGVSIPPGQLLVSLYAGIG
                     RDPDLYGSTADRYDITRVVSQQVAFGTGSHYCLGAPLARLESRIALCELFSRHPEVRLA
                     VPDRDLRQVPSMFTNSPRALPVHIDVHADSHR"
     misc_feature    3198..3212
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.0"
                     /tool="antismash"
     gene            complement(3224..4273)
                     /locus_tag="IEZ14_RS41085"
                     /old_locus_tag="GCM10010177_82970"
     CDS             complement(3224..4273)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     PKS-like: ksIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291676.1"
                     /locus_tag="IEZ14_RS41085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_82970"
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="WP_179837917.1"
                     /sec_met_domain="ksIII (E-value: 7.6e-92, bitscore: 296.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEPNIYIAACATWLPPLLPVAQAVADGQCDEKLAQRTGMVSVAV
                     AGTEAAPEMAGRAARIALRRARYDGADIALILHANFFYQGHDVWAPASYVQRVAVGNDC
                     PAVEIRQASNGGMAAVDLARAFLIAGPGREAALVTTGDKFSPPAFDRWRSDPGTVYADG
                     GTALALSRRRGFGLLRSLVTVSDPELEGMHRGDDSFGPAPLSARSIVDLDACQRDFLAS
                     AGMRRVAARVCAGQEAVLAKALAQAEVKLADIDRVVLPHLGLHRLNGSYLRRLGLKPEI
                     TTWPWSRHIGHLGAGDPFAGLEHLMLTGALGAGDRCLVISVGAGFTWTAAVIDILERPT
                     WADHAARSP"
     gene            complement(4488..5507)
                     /locus_tag="IEZ14_RS41090"
                     /old_locus_tag="GCM10010177_82980"
     CDS             complement(4488..5507)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013087.5"
                     /locus_tag="IEZ14_RS41090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_82980"
                     /product="methyltransferase"
                     /protein_id="WP_179837916.1"
                     /transl_table=11
                     /translation="MEPAQDSIAAATIILDQTVGMYRTAALAVAARAGVADHLDRPRTV
                     VELAERTGVNAAALRRVLRMLASQDIFREDDQGLYHLTPAAEFLRSGASRSLRDAVIMF
                     SEDYMWRSASHLTAAVETGSSSFEQLYSKPFFEWLGANPDAASTFHASMVQLSETGIER
                     IIDTYDFPATGTVVDVGGGRGALLREILEKNQHLNGVLQDDRSALREHVLDTPQTADRW
                     RLEEGSFFESVPVGGNVYVLKHIIHNWGDEQSTAILRNCRSAMKDDARVLIIDPVLPEP
                     GNPHFGWALDLVMLSTLTGKERTRQEFEAILNDAGLRISNIFPVQTLGEYSIIEAVAA"
     gene            complement(5568..6650)
                     /locus_tag="IEZ14_RS41095"
                     /old_locus_tag="GCM10010177_82990"
     CDS             complement(5568..6650)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013087.5"
                     /locus_tag="IEZ14_RS41095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_82990"
                     /product="methyltransferase"
                     /protein_id="WP_218935430.1"
                     /transl_table=11
                     /translation="METQPPGSIKDLMEICTGFWAFKSIVSAQELGLFDILHEAGAQPI
                     SDLARRLDVQERPAETLVTALASLGLLKVDDGRYGNSPLAETYLVRGGRYYFGGWIAHL
                     DQRNYPGWAHLTEAIRTNRPMGWQSGHEAETPFDIVDPELTSQFQDGMHSLSRHSAASA
                     AQRIDLSGARTLLDVGGGTGAWAIELASANPQLTITIYDLPPVCDLARAKIEAEGLSSR
                     ISTISGDFRTQELPSGYDTVLLSMILHDWTPDECLRLLNKCHRALAPGGRAIISELLVD
                     DDKSGPADAALMSLNMLVATQGRNYTAAEYNAWLLEAGFEAPELEPIDTPSCNGLISAR
                     KKAPVDGSPAERHVVDTSAS"
     gene            complement(6776..7048)
                     /locus_tag="IEZ14_RS41100"
                     /old_locus_tag="GCM10010177_83000"
     CDS             complement(6776..7048)
                     /GO_function="GO:0000035 - acyl binding [Evidence IEA]"
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (30-66). E-value: 1.5e-07.
                     Score: 23.6. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS41100_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.5"
                     /locus_tag="IEZ14_RS41100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_83000"
                     /product="acyl carrier protein"
                     /protein_id="WP_179837914.1"
                     /sec_met_domain="PP-binding (E-value: 1.4e-07, bitscore:
                     21.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METDARLNEKDLIDMLPTDGAPAPAHDSETTGSRSLADFGYDSIA
                     MADLMSQIELRYEVKLPDPLLGELLKVSITQATSLINQHITAAAG"
     aSDomain        complement(6851..6958)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS41100_PP-binding.1"
                     /evalue="1.50E-07"
                     /label="IEZ14_RS41100_PP-binding.1"
                     /locus_tag="IEZ14_RS41100"
                     /protein_end="66"
                     /protein_start="30"
                     /score="23.6"
                     /tool="antismash"
                     /translation="TGSRSLADFGYDSIAMADLMSQIELRYEVKLPDPLL"
     gene            complement(7087..7335)
                     /locus_tag="IEZ14_RS41105"
                     /old_locus_tag="GCM10010177_83010"
     CDS             complement(7087..7335)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2clf"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014819.5"
                     /locus_tag="IEZ14_RS41105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_83010"
                     /product="hypothetical protein"
                     /protein_id="WP_229810783.1"
                     /sec_met_domain="t2clf (E-value: 8e-30, bitscore: 92.9,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSPKTLTGKMGAGGAALDVATALLALHHQVIPPTARRGSIPQPR
                     GIDMVYDRPRETRLSNTLVLARGHGGHNSAIVVGALD"
ORIGIN
        1 aggcaacggc cgcgaagtcg acaaactggt ccgctcggcc atgaccgccc acgcacgccg
       61 caccgaacaa ctcgccagca ccggcgccca actcaccacc gaacaactcg ccaccctcct
      121 ccccgaagac atcacctagt cctcgggtgt gacgctccac caggcggccg tgtcgggcgg
      181 gaggatcgcg tcccggcctg ccgtacggat cggcccgctg ctcagcagga gcgagccggg
      241 ggccgagaac cggatgctct ggtcgccggt gttcaccgcg cacagcattc gcgggccgcc
      301 ggaatgaccg gtgcgctcga acaccaggca acccggcggc tcgtccagcc agcgcagctc
      361 gccgtcaccc aaagccggat ggtcgcgccg gatccggagg gcctggcggt agagggtcag
      421 catggaagcg tcgtcgtccc gctgcgcctc cacggtgagc gacttccatt cggccggcga
      481 cggcagccag ccaacgcccg acccgaaccc gaacgggggc tcgtagccgg accacggcag
      541 cggaacccgg caaccgtccc ggccgccctt gatgctctcc agctgcgggt cggtcagcag
      601 ctcggccggg atgtcgagca cctcgggcag accgagctcc tctccctggt acacgtacgc
      661 cgaaccgggc aacgccaggg tgagcagcgc cgccgctcgg gcccgccgct gtccgaggtc
      721 gccgtcgccg tagcgggtga catgccgctg gacgtcgtgg ttggacagca cccaggtggt
      781 gggtgcgccg acggagccgg ccgaggccag ggacttctcg atcaccgagc gcagtgcacc
      841 ggcgtcccag tcggtcctca tgtagtcgaa gttgaaaacc tggtgcagct cgtccggccg
      901 tacgtagttg gccagccgat cgagcgaggg cgcccatgcc tcagcgaccc cgatccgctc
      961 tccggggtag gagtcgagca gccgtcgcca gcctcgatgg atctcgtgca ccccgtcctg
     1021 gtcgaagtac ggcaccttgc cgcgtcccag cagccgcgac tgcggcgcgt caccgacgtc
     1081 gggcagccct gccgccttga ccatgccgtg cgccacgtcg atgcggaatc cgtcgaccgc
     1141 caggtccagc cagaatcgca ggacgtcgtc gaactcggcg cgcacgtccg ggtgttccca
     1201 gttcaggtcg ggctgctgcg gagcgaacag gtgcaggtac cactgcccgt caggcagccg
     1261 ggtccacgcg ggaccaccga agaccgactc ccagtcgttg ggcggcagtt cgccggcggc
     1321 tcctcgtccc ggccggaaga cgtagcgctc ccgcgccgga ccggagatcc cggcggccag
     1381 cgcctcctgg aaccagacat gctggtcgga ggtgtggttg ggcacgatgt caacgatgac
     1441 gcgcagcccc aggccgtgcg cgtcctcgat caggtccgcg gcgtcggcga gcgtgccgaa
     1501 cgtcgggtcc accgagcggt agttcgtgac gtcgtagccg ccgtcgacca tcggcgactc
     1561 gtagaacggc gtgatccaca atgcgtccac gccgagcgag gccagatagg gaagccggtc
     1621 tcggacgccg gccaggtcgc cgatgccgtc cccgtcggcg tcggcgaagc tgcgtacata
     1681 gatctggtag atgaccgcgt cacgccacca gcgtgtatcg cccaccagag ggctggagga
     1741 ctccgcaggg cgtcgcaccc cgcgcgtcgg tgaaggagat gtcgatgaca gatccatacg
     1801 agggaccgtc tggagatcca tttgatacgc actggaggtg accgatcaac gtgatctccg
     1861 cggcggccgc ctgccgctca acgatgcgag tctgcgtgaa cgtcgatgtg gacgggcagt
     1921 gcgcgggggc tgttggtgaa catggagggg acttgccgga ggtcgcggtc ggggacggcg
     1981 agccgcacct cgggatggcg gctgaacagt tcgcacagcg cgatgcggga ttcgaggcgg
     2041 gccagaggcg cgccgaggca gtagtgactc ccagtaccga aggccacctg ctgggagacg
     2101 acgcgggtga tgtcgtaccg gtcggccgtg gaaccgtaga ggtcggggtc gcggccgatg
     2161 ccggcgtaca agctgaccag gagctgtccg ggcggaatgc tgacgccgcc gatggggatc
     2221 gtgtcggtcg tgtagccggc caccagatag ttgatcacac cgtcccaccg cagggtctcc
     2281 tccaccacgg cgctccagcc gtcgggtggg agtgctctgg cgtgagccag ctgatcgggg
     2341 tgggtgagca gggcgcggac cgcgttggtg atcagattga gggtggtctc gtgcccggcg
     2401 atcaccagtg accacagggt gtcgagcagc tgccggtgga gcagagcgcc gccgccgttg
     2461 tcgcgcacag tgatcaggct gctggtcaga tcggcaccgg ggcgggcgag gcggagttcg
     2521 accagttcgt ccagcagatc caggcggccc tgttcgaggg cggcggcgtc ttgcgcggtc
     2581 ttggcgtcgg tgcggagcag cgtgtcgacc aggttccgga attgcggccg catggattcg
     2641 gggatgccga gcaggtggca gatgacaccc atggggaccg tgtaggcgta gtgggcgcgc
     2701 aggtcgacgg cgccgtccgg gccgcggtgg ccgggcaggg cgtccaaggc gtgccggacg
     2761 atgcgggtga tgtcggcgct caactgttcc acgcgtttgg cggtgaacac cgagctgatc
     2821 attcggcggt gatgctggtg ttcggggtcg tccaggaaga acatgttggt caggccgatg
     2881 aacatgcccg ccaggggcca gtcggcgggg acgaggccgt cgcggtaggc ggcccagtgc
     2941 cggtgcgcga cccgggacac ggcggggtgc cggctgagcc ggctgagcag gtcctggcgg
     3001 gtgaccatcc aggcgtgcac ggtgtcgggc aggccgggca gcaccgttcg cacgaccgga
     3061 ccggcggcgc gcagccgggc catctcggca tgcgggtcgg cgccgagggg gtcgatggtg
     3121 acgatcggag ccgactgctg ccggtcgctg gtctgggcgt cgaggtgtgt cactggcttc
     3181 ctccaatgct gtggaccgga tggcgtcgga cacgcggggg gcttcacggc gatctcgccg
     3241 cgtggtcggc ccaggtcggg cgctccagga tgtcgatcac ggcggccgtc cacgtgaagc
     3301 cggcgccgac gctgatgacc aggcaccggt ccccggcgcc gagggcgccg gtcagcatca
     3361 ggtgctccag tccggcgaag gggtctccgg cgccgaggtg cccgatgtgc cgactccacg
     3421 gccaggtggt gatctccggc ttgaggccga gtcttcgcag gtagctgccg ttgagccggt
     3481 ggaggcccag gtggggaagg acgacgcggt cgatgtctgc gagcttgacc tccgcttgcg
     3541 ccagcgcctt ggcgagcacc gcttcctggc ccgcgcacac gcgcgccgcc acgcgtcgca
     3601 ttccggcgct cgcgaggaag tcacgctggc atgcgtcgag gtcgacgatg gaacgcgcgc
     3661 tcaagggggc gggcccgaag gagtcgtcgc cgcggtgcat gccctccagc tctgggtcgc
     3721 tgaccgtcac caggcttcgc agcagcccga atcccctccg ccgggacagc gcgagcgcgg
     3781 tcccgccgtc ggcgtacacg gttcccgggt cgcttcgcca ccggtcgaag gcgggcgggc
     3841 tgaacttgtc gcccgtggtc accagcgcgg cctcgcggcc gggaccggcg atcaggaagg
     3901 cgcgcgccag atcgaccgcg gccatgccgc cgttggacgc ctggcgtatc tccaccgccg
     3961 ggcagtcatt ccccacggcg acacgttgga cgtacgacgc cggggcccac acgtcatggc
     4021 cctggtagaa gaagttcgca tgcaggatca acgcgatgtc cgcaccgtcg tagcgcgccc
     4081 ggcgcagcgc gatccgagcg gccctgcccg ccatttccgg tgccgcctcg gtaccggcca
     4141 cagcgaccga caccattccc gtgcgctgtg cgagtttctc gtcgcattga ccgtcggcga
     4201 cggcctgcgc gaccggcagc aatggcggca gccaggtcgc gcaggccgcg atgtagatgt
     4261 ttggctcgct caccgagcac cctccatgcc ctttccaggg agtaccggga agtgcggccc
     4321 ttgcggatgg ccgtgcctgc aaggaccagg ttctcccggg actcggccct cccgggcgga
     4381 cgaacgtcgt gacggcgggt gcggtcaggg ccgatggggc cggccggaaa cgcgatgacc
     4441 tctgcgaggt cgggtgtggc ctggccgcag ggcctgggct gcccttccta tgccgcgacg
     4501 gcctcgatga tcgagtactc tccgagcgtc tgcacgggga atatgttgct gatgcgcagg
     4561 cccgcgtcgt tcagtatcgc ttcgaattcc tgccgggtgc gttccttgcc ggtgagggtg
     4621 ctcaacatga ccaggtcgag ggcccagccg aagtgagggt tgccgggttc cggcagaacc
     4681 gggtcgatga tcagtacgcg ggcgtcgtcc ttcatcgccg accggcagtt cctcaggatg
     4741 gcggtggact gctcgtcgcc ccagttgtgg atgatgtgct tgaggacgta gacgtttccg
     4801 ccgacaggga ccgactcgaa gaagcttccc tcttccaggc gccagcggtc ggccgtctgc
     4861 ggcgtgtcga ggacgtgctc gcgcagcgcg ctccggtcgt cttggagaac gccgttcagg
     4921 tgctggttct tctcgaggat ctcgcggagc agcgcgccac gtcccccgcc gacgtcgacc
     4981 acggtgcccg tggcaggaaa gtcataggta tcgatgattc tttcgatgcc cgtttcggag
     5041 agttgaacca tcgacgcgtg gaatgtcgag gcggcgtcgg ggttcgcgcc cagccattcg
     5101 aagaagggct tgctgtacag ctgttcgaag gaactggatc ccgtctccac cgcggcggtc
     5161 aggtggcttg ccgatctcca catgtagtct tcggagaaca tgatcacggc gtcgcggagt
     5221 gaccggctcg cgccggagcg aaggaattcg gccgcgggcg tcaggtggta caggccctgg
     5281 tcgtcctcac ggaagatgtc ctgggaggcg agcatgcgga ggacccggcg cagcgccgcg
     5341 gcgttcacgc cggtcctctc ggccagttcg acgaccgttc gcggccggtc cagatggtcg
     5401 gcgacgccgg ctctggccgc gacggcgagg gctgcggtcc ggtacatccc gacggtctga
     5461 tcgaggatga tcgtcgcggc ggcgatgctg tcctgagctg gttccattcc ggtcctccgt
     5521 actcgtcttc tcggatttgc cctggctctc gggctggtct gcgcctgcta ggaggcggac
     5581 gtgtcgacga cgtgtcgttc cgccggcgaa ccgtcgacgg gggccttctt acgcgccgag
     5641 atcaggccgt tgcaggaagg ggtgtcgatc ggctccagtt ccggcgcttc gaaccccgct
     5701 tccagcagcc aggcgttgta ctcggcggcg gtgtagttgc ggccctgggt ggccacgagc
     5761 atgttgaggc tcatcagcgc cgcgtcggcg gggccggatt tgtcgtcgtc gacgagcagc
     5821 tcgctgatga tcgcccggcc gccgggtgcc agcgcccggt ggcatttgtt caggagccgg
     5881 aggcattcgt cgggcgtcca gtcgtgcagg atcatcgaga gcagaacggt gtcgtatccc
     5941 gacggcagct cctgggtgcg gaagtccccg gagatcgtgc tgatccggct gctcagcccc
     6001 tccgcctcga tcttggcccg ggccaggtcg cacacgggcg gcaggtcgta gatggtgatc
     6061 gtcagctgcg ggttcgccga ggcgagctcg atggcccagg ccccggttcc gccgccgacg
     6121 tcgagcagcg tccgggcgcc cgacaggtcg atccgctggg cggctgaggc cgccgagtgc
     6181 cgggagaggg agtgcatccc gtcctggaac tgggaggtca gctccgggtc gacgatgtcg
     6241 aagggggttt ccgcctcgtg gccggattgc cagcccatgg ggcggttggt tcgtatcgct
     6301 tcggtcagat gggcccaccc ggggtagttg cgttggtcga gatgggcgat ccagccgccg
     6361 aagtagtacc tcccgcccct gaccaggtag gtctcggcga gcgggctgtt gccgtaccgg
     6421 ccgtcgtcga ccttgagcag tccgagcgag gccaaggcgg tcaccagcgt ttccgcgggc
     6481 cgttcctgca cgtcgagccg ccgtgccagg tcactgatcg gttgggcgcc ggcctcgtgc
     6541 aggatgtcga agaggccgag ttcctgcgcg ctgacgatgg acttgaatgc ccagaagccg
     6601 gtgcagatct ccatcaggtc cttgatggag ccgggcggtt gtgtttccac ggtcactgat
     6661 ttcctctggt tcgcgacgtc ccgttcggcg atggcactgt gggtcatcgc ctccgggagc
     6721 cgttgccggg tgtgctcgca tggccgcgag cgccgcacgc gggtcgggcc cggtgtcacc
     6781 cggccgcggc cgtgatgtgc tggttgatca ggctggtggc ctgggtgatg ctgaccttga
     6841 gcagttcgcc gagcagggga tcggggagct tgacctcgta gcggagctcg atctgggaca
     6901 tcaggtcggc catcgcgatc gagtcgtatc cgaaatcggc gaggctgcgg ctgccggtcg
     6961 tctcgctgtc gtgggcgggc gcgggtgcgc cgtccgtcgg cagcatgtcg atgaggtctt
     7021 tctcgttcag ccgggcgtct gtttccatgg ccggtgccct ttctaggcgc agacgtggtg
     7081 tgcgggtcag tccagggcgc cgaccacgat ggccgagttg tggccgccgt ggccgcgggc
     7141 caggaccagg gtgttgctca gccgggtctc gcggggacgg tcgtagacca tgtcgatgcc
     7201 ccgcggctgc ggaatgctcc cccgcctcgc ggtcgggggg atgacctggt ggtgcagcgc
     7261 cagaagcgcg gtggcgacgt cgagggcggc tccgccggcg cccatcttcc cggtgagggt
     7321 cttgggtgag gtcaccggca ccgggtggta aaaaagtcca ggtaggggac ggtgccgtcg
     7381 gcgaagatca cgtcgatgtc ggcggggtcg acctgcgcgt cggcgagggc gg
//
