LOCUS       NZ_BMRD01000010        10867 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence10, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000010
VERSION     NZ_BMRD01000010.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 283096)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 146561
            Orig. end                         :: 157428
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10867
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="2"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..10867
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10867
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5867](-)"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5867)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            complement(152..1327)
                     /locus_tag="IEZ14_RS23770"
                     /old_locus_tag="GCM10010177_48000"
     CDS             complement(152..1327)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1282:
                     extracellular ligand-binding receptor"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF024825.5"
                     /locus_tag="IEZ14_RS23770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48000"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_179835562.1"
                     /transl_table=11
                     /translation="MTRPIARRRRLAAFAVAAAAALPAAACNGGTQSGEIKIGFITANT
                     GPVAFAGQTFGNGTQLAIDQLNREGYLGGGRKIKLVRKEGAESPARAVEQAKQLAGDQG
                     VVGAICCILSPVAGAVKPITTAAKLPLDIYGATDLGLEDPPYVVRTATLPQDANKSLAQ
                     KVAQQAKPASVAYAVTQDNSGWVSQLKSFQQGFAGTAVRDLGTVNTLAAQTDFSGAAGQ
                     LMAKNPEAIVIAALQQSSISLIKALRSRGYKGLIVSAETIGSPGAFKAAGRTLADVPFP
                     VYFFSGKASAQGQKFAQTYKAKYGTDPDDYAAQGYMAAYMMATAVKDSGKDVTRESVAK
                     ALSDIKKLDGTIYGTVQFQQGQLHAPSSIVHITWTADGRQTEWAPSGPAAS"
     gene            complement(1413..3134)
                     /locus_tag="IEZ14_RS23775"
                     /old_locus_tag="GCM10010177_48010"
     CDS             complement(1413..3134)
                     /NRPS_PKS="Domain: Abhydrolase_1 (314-430). E-value:
                     2.5e-17. Score: 55.2. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS23775_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.5"
                     /locus_tag="IEZ14_RS23775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48010"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_218935213.1"
                     /sec_met_domain="PF00561 (E-value: 1.6e-12, bitscore: 41.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.9e-13, bitscore:
                     44.1, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGRHVDTGHGQIRVWTTDDRPGVLVLPGIRWSGRPLAEEIALGA
                     PELPVAVADLPGVAGSAPGAAAGVGPAADALAQVAGALGVHAIVGLDMSCDLAAAVAGR
                     VPHCGELVQVDPGYLAAAREMPPPDLTPRADGTHLSRLWQMVRNSHLFPPFSTRRAVPG
                     RAEAPSAAALDRTVVAFAEHPEAFTALWQTWCRAGEDPFAGTRPAAVVVNSEDTGPTPV
                     VRWTGRLSRAHGHPAPVQAPAGPPSAPAHDRARRSLPLAVEPSPATASSCAAASCATAP
                     SGPAIVRGTAIRRDFLDTSAGQVHVRVAGDPEAPPLIALHSAPGSARLLEPLMRGLAPD
                     FHVIAPDYPGNGESDKPWRDGVDIGRLADAMAEVAERRADGPVHLWGTHTGAVIALELC
                     VRRPDLVRRAVLEAPPLLPAEFTSDILENYLPPLRADRFGSHLVRAWGMRHDMFLFWPW
                     YRDRTAADRPLRVPRARELHDWTVGLLQSGPTYHLSYAAAFRYPTRDRLPELTVPALIC
                     AGPDDMLADNLPALRSAAPDGVTLASTPATCWYPDQDPAAVAETVRLYRDFLTAVP"
     aSDomain        complement(1845..2192)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS23775_Abhydrolase_1.1"
                     /evalue="2.50E-17"
                     /label="IEZ14_RS23775_Abhydrolase_1.1"
                     /locus_tag="IEZ14_RS23775"
                     /protein_end="430"
                     /protein_start="314"
                     /score="55.2"
                     /tool="antismash"
                     /translation="PPLIALHSAPGSARLLEPLMRGLAPDFHVIAPDYPGNGESDKPWR
                     DGVDIGRLADAMAEVAERRADGPVHLWGTHTGAVIALELCVRRPDLVRRAVLEAPPLLP
                     AEFTSDILENYL"
     gene            complement(3136..3675)
                     /locus_tag="IEZ14_RS23780"
                     /old_locus_tag="GCM10010177_48020"
     CDS             complement(3136..3675)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611200.1"
                     /locus_tag="IEZ14_RS23780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48020"
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="WP_179835561.1"
                     /transl_table=11
                     /translation="MAAGAPVLPDVLRGRVAWVFGDDFDVDLIVGVENIKYYDPDKLRA
                     VCMRSFDPEFVDRVRPGDVVVGGRNFGYGHPHYPPLVALRNAGISAVVAESFSPGFWRG
                     ETYNGMPLVTVPGIVAAVRRFDAVEVDWRKATVRLADGTVLAGSPPSDRVVKVLEAGGS
                     LNLLLREHGRPAPAER"
     gene            complement(3675..4991)
                     /locus_tag="IEZ14_RS23785"
                     /old_locus_tag="GCM10010177_48030"
     CDS             complement(3675..4991)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611201.1"
                     /locus_tag="IEZ14_RS23785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48030"
                     /product="aconitase family protein"
                     /protein_id="WP_179835560.1"
                     /transl_table=11
                     /translation="MTRFDDATRTAATARTATAQTITEKILARAAGLGTARAGQNHAFR
                     PDYMVAYDFPGYTDLMFKQMHDDFGITTVDDPERYLIFIDHMLSNRDGREREVHDVTRR
                     WGDFYGITVHEGRGIGHQVAAEMGYALPGTFLIHFDGHISGLGAFGALGWGVRRDLLEA
                     WVSGQIFLDVPASTRFHLTGAFAPGVDNRDLIHEIISRVGADGCAHQVMEFTGPGAEAM
                     SIGRRQGLCGMAMFTGAVSAIFNPDDLALEYARSVARKEFEPLTSDPDASYTATHHIDL
                     DALQPRIVLPGSARAANTLGVEDAAGTPITRAFIGSCASGRIEDIRAAAQILRGRRVAP
                     GVRLNVVPTSEVIHRQAEEEGLLDVLREAGADVAISSCDFCFGYAKPLQEGENCISTGV
                     LNISGRMGSPGADIYMGSAYTVAASAVAGRITDPREVMA"
     gene            complement(5001..5867)
                     /locus_tag="IEZ14_RS23790"
                     /old_locus_tag="GCM10010177_48040"
     CDS             complement(5001..5867)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611202.1"
                     /locus_tag="IEZ14_RS23790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48040"
                     /product="oxaloacetate decarboxylase"
                     /protein_id="WP_179835559.1"
                     /sec_met_domain="phosphonates (E-value: 1e-46, bitscore:
                     153.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.1e-52, bitscore:
                     172.0, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATRLRELMDDDVVFAPGVWDGLSARLAEQAGFPALCASGFAIS
                     AALGLPDAELYTMSENLDAVRRIIETSDLPVIADIDTGYGNAVNAARTARRFAAAGVAA
                     VFMEDQESPKRCPICVGDPVPVISLEEAAGKIRAVRDAVGDRLVVIARTDTAGEEALGR
                     AEAYAAAGAEMIMPVTKTFAGVDDWARCRERAGVPLMATLTASTWVERDFTPEVLREVG
                     VRLALLPTQILLSATGAMRETLRRLESGEPPAEVSADALPHHDFVDLIGFADIERAQEK
                     YLPVAGA"
     misc_feature    complement(5884..5899)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.01"
                     /tool="antismash"
     misc_feature    5890..5901
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     gene            6079..6981
                     /locus_tag="IEZ14_RS23795"
                     /old_locus_tag="GCM10010177_48050"
     CDS             6079..6981
                     /GO_function="GO:0004061 - arylformamidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016115.5"
                     /locus_tag="IEZ14_RS23795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48050"
                     /product="cyclase family protein"
                     /protein_id="WP_179835558.1"
                     /transl_table=11
                     /translation="MDTFWPGVGANWGRWGERDERGTLNLVGPEKIRSAAASVRSGRAI
                     SLARTLDDRLPAYYPAAGESAFEHRMITWWGSNAGGDVQAASDRVSSECHGLEITHMDA
                     LSHIGHRGRGYGGRDFASMAGPDGVAECDITAAAPVVTRAVLADIPRLRGVDHLPAGTA
                     VGHDELAEAAPGLGPGDALVVRTGRWNAPSPAPDPGDRYGHLSGLHPDALRFVADRDAA
                     VVGTDGPGDAFPVAVEHCRLPVHVLALVYLGVHLVHSMVLDELAAALAAEDRTDFMLVL
                     GPLVVPGATGSPITPVAVL"
     gene            7171..8799
                     /locus_tag="IEZ14_RS23800"
                     /old_locus_tag="GCM10010177_48060"
     CDS             7171..8799
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013086.5"
                     /locus_tag="IEZ14_RS23800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48060"
                     /product="FAD-binding protein"
                     /protein_id="WP_179835557.1"
                     /transl_table=11
                     /translation="MERTVDFLCIGGGLGGLAGAVRAHDLGADVLVVERSDMVGGVAAY
                     SGGFVWVGANDLTGSADSLAATEAYLDHVQGQGRPVDRPARRAYLELAVEATRWYREAG
                     VPFEVIRNCPDLYHPAPGSTPEGRLLECVVPGATLGPWRDRLRPSPYYVTGIPRNDLYS
                     ESSTDPNEVDRWQRRGADRDLLTHGSGLSAAFIRAALTERGIDCLPRHRAIRLLMDGDA
                     VKGAVLDGPDGRVTVRASRGVLIAAGGYGHAPDAWELEDVPALVESAPPVVEGDNLLLA
                     QQAGAATVRGADPFFSVGFHFPGETHPGGDLPLCRPLLEHLGLPHSMIVNRDGRRFGDE
                     AYYGALIGALRRFDGRSRQWSNYPCWFLADEEFRRRYRLGRYAPGEPWPDEIVRADSPE
                     DLARATGIDPDGLAATFASFNKGAERGEDPEFHRGSLPFVRRAYGDPLHEPNPNLGPLA
                     TPPFYALPLSIVGFGMGTLGLWIDGSARVRRRDGAVVPGLYATGNAAATKELKGYVTGL
                     ANARNYTYAYAAATHALGSSAALDR"
     gene            8843..9622
                     /locus_tag="IEZ14_RS23805"
                     /old_locus_tag="GCM10010177_48070"
     CDS             8843..9622
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611204.1"
                     /locus_tag="IEZ14_RS23805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48070"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_179835556.1"
                     /transl_table=11
                     /translation="MTPDDREGVDPKNVVVSVLKACQLMECFTREKPNLTLADLTVATG
                     MNKTTVHRLAATLIQAGWLTRGEGATYRLTMRPFRVGAVALAELSLRSEAVPFLRGLAD
                     RFGDTAYLMIPGPDGAVCVDIFQGNNPVRVNTVGVGTVLPYHTAAGPVVMLAFSDELRE
                     RWLARTLDQYTSNTLVDAPSLKDRLDKIVRDGYAISEEDYLYGVGAVAAPVFDAQGELV
                     ATLSLGGVNDGFRGSRLLEIIEAVLSSSRELSSRLGH"
     gene            9669..10097
                     /locus_tag="IEZ14_RS23810"
                     /old_locus_tag="GCM10010177_48080"
     CDS             9669..10097
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890532.1"
                     /locus_tag="IEZ14_RS23810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_48080"
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="WP_229810363.1"
                     /transl_table=11
                     /translation="MHEERKTMDARYTAVATANGRDGRAVTSDGRLDVSLAPPRELGGS
                     GEGTNPEQLFAAGYAACFASALSAVARASRHDVRDVSVTAEVGLHGDTEKGFGLGVVLR
                     VELPDDLADGEQLVERAHQICPYSNATRGNIPVDLVIE"
     misc_feature    complement(10357..10371)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
ORIGIN
        1 ccaggtggcg tagacgccct gcgcgaggtt caggatccgc atcgttgaga acacgacccc
       61 gaaacccacc gcgaacagcg cgtacacggc gcccagcgcg attccgttga cgagttgctg
      121 gaggaacagc atggctcagc tcacccggct ctcaggatgc ggccgggccg gacggcgccc
      181 attcggtctg gcggccgtcc gcggtccagg tgatgtggac gatgctgctg ggcgcgtgaa
      241 gctggccctg ctggaactgc accgtgccgt agatggtgcc gtcgagcttc ttgatgtccg
      301 acagcgcctt ggccacgctt tctcgcgtga cgtccttgcc cgaatccttc acggccgtgg
      361 ccatcatgta cgcggccatg tagccctggg cggcgtagtc atcgggatcc gtcccgtact
      421 tggccttgta ggtctgagcg aacttctggc cctgggcgct ggccttcccg ctgaagaagt
      481 acaccgggaa aggaacgtcc gccagcgtcc tgcccgcggc cttgaacgcc ccggggctgc
      541 cgatcgtctc ggccgacacg atcagcccct tgtagccgcg gctgcgcagt gccttgatca
      601 gggagatgga cgactgctgc agcgcggcga tgacgatggc ctcggggttc ttggccatga
      661 gctggcccgc cgcgccactg aagtcggtct gggcggcgag ggtgttgacc gtcccgaggt
      721 cccggacggc ggtgccggcg aagccctgct gaaagctctt caactgggag acccagccgg
      781 agttgtcctg ggtgaccgcg taggcgaccg acgcgggctt ggcctgctgg gccaccttct
      841 gcgcgaggct cttgttggcg tcctgcggaa gggtcgcggt gcggaccacg tacggcgggt
      901 cctccaggcc gaggtcggtg gcgccgtaga tgtcgagcgg cagcttggcg gccgtggtga
      961 tgggcttcac cgcgcccgcc accggcgaca ggatgcagca gatcgctccc acgacgccct
     1021 ggtcgcccgc gagctgcttc gcctgctcga cggcccgcgc cggggactcg gccccctcct
     1081 tccgcaccag cttgatcttg cgtccaccgc cgaggtagcc ctcccggttc agctggtcga
     1141 tcgcgagctg ggtgccgttg ccgaacgtct ggccggcgaa agcgacgggc ccggtgttcg
     1201 ccgtgatgaa cccgatcttg atctcgccgc tctgcgttcc gccgttgcac gccgcggccg
     1261 gcagggcggc ggccgcggcg acggcgaacg cggccagccg ccgtcggcgg gcaatgggtc
     1321 gtgtcatggg gtctcctcag aagggccgcc gagaaggcgg ggctgggaaa gaagcgctgg
     1381 tcatgggatg gatggaggcg acggggccgc cgtcagggga cggcggtgag gaagtcccgg
     1441 tagagccgga cggtctccgc gacggccgcg ggatcctggt cggggtacca gcaggtcgcg
     1501 ggtgtggagg cgagggtgac gccgtccggt gccgcggagc gcagggccgg gaggttgtcg
     1561 gccagcatgt cgtcggggcc ggcgcagatg agcgctggca ccgtgagctc cggcagccgg
     1621 tcccgggtgg ggtaacggaa ggccgccgcg tagctgaggt ggtaggtcgg gccgctctgg
     1681 agcaggccca cggtccagtc gtgcagttcg cgggcgcgcg ggacccgcag cggacggtcg
     1741 gcggcggtgc ggtcccggta ccacggccag aacaggaaca tgtcgtgccg catgccccac
     1801 gcccgcacga ggtgggaacc gaaccggtcc gcccggaggg gcgggaggta gttctcgagg
     1861 atgtcggagg tgaactccgc cggcagcagg ggcggtgcct caaggacggc gcgccgtacc
     1921 aggtcggggc ggcgcacgca cagttcgagc gcgatgaccg cgccggtgtg ggtgccccac
     1981 aggtgcaccg ggccgtccgc ccgccgctcg gcgacctccg ccatcgcgtc ggcgagccgt
     2041 ccgatgtcga ccccgtcgcg ccagggcttg tccgactcgc cgttgcccgg gtagtccggc
     2101 gcgatgacgt ggaagtccgg cgccagcccg cgcatcagcg gttcgaggag ccgcgcggat
     2161 ccgggcgccg aatgaagcgc gatcaggggt ggcgcctcgg ggtctccggc gacgcgcaca
     2221 tggacctggc ccgctgatgt gtccaggaag tcgcggcgga tcgccgttcc ccggacgatg
     2281 gccggacctg acggagccgt tgcgcacgag gccgccgcgc acgatgatgc cgtcgcgggc
     2341 gacggctcca ccgccagcgg cagcgaccgc cgggcgcggt cgtgcgcagg tgcggagggc
     2401 ggaccggccg gcgcctggac gggcgccggg tggccgtgag ccctggaaag cctgccggtc
     2461 caccggacga cgggcgtcgg gccggtgtcc tcggagttga ccacgacggc ggccggacgg
     2521 gtgcccgcga acgggtcctc acctgcacgg caccacgtct gccacagagc ggtgaaggcc
     2581 tccgggtgtt ccgcgaacgc gacgacggtc cggtcgagcg cggccgcact gggcgcttcg
     2641 gcccggcccg gcaccgccct gcgcgtcgag aacggcggga acaggtggga gttccgcacc
     2701 atctgccaca gccgcgacag gtgggtcccg tcggcccgcg gcgtgaggtc gggcggcggc
     2761 atctcccgcg ccgccgcgag gtagcccggg tccacctgga ccagttcccc gcagtgcggg
     2821 acgcgtccgg cgacggccgc ggccaggtcg cagctcatgt ccagcccgac gatggcgtgc
     2881 acgcccaggg ccccggcgac ctgcgcgagc gcgtcggccg ccgggccgac gccggccgcg
     2941 gctcccggag cggatcccgc cacacccggc aggtcggcca ccgccaccgg gagttccggg
     3001 gcgccgagcg cgatctcctc cgccaggggc ctgcccgacc agcggatccc cggcagtacc
     3061 aggacgcccg gacggtcgtc ggtggtccag acccggatct ggccgtgccc ggtgtcgaca
     3121 tggcgtcccc gcatgtcagc gctccgccgg ggccggccgg ccgtgttcgc gcagcagcag
     3181 gttgaggctg ccgccggcct ccaggacctt gacgacgcgg tcgctcggcg gggagccggc
     3241 cagtacggtg ccgtccgcga gtcgcaccgt ggccttgcgc cagtccacct cgacggcgtc
     3301 gaagcgacgc accgccgcga cgatgcccgg gacggtcacc agcggcatcc cgttgtaggt
     3361 ttcgccgcgc cagaacccgg gggagaagga ctcggcgacc acggcgctga tgcccgcgtt
     3421 acgcagggcg accagcggcg ggtagtgcgg atgcccgtag ccgaagttgc gtccgcccac
     3481 caccacgtcg ccgggccgca ccctgtcgac gaactccggg tcgaacgacc gcatgcagac
     3541 ggcgcgcagc ttgtccgggt cgtagtactt gatgttctcc acaccgacga tcaggtcgac
     3601 gtcgaagtcg tcgccgaaca cccaggcgac gcgtccgcgc aggacgtcgg gcaggaccgg
     3661 ggccccggcg gccatcacgc catcacctcc cgcgggtcgg tgatccgccc ggcgacggcg
     3721 ctggccgcga cggtgtaggc cgaacccatg tagatgtcgg cgccgggact gcccatgcgc
     3781 ccggagatgt tcagcacgcc cgtggagatg cagttctcgc cctcctggag cggcttggcg
     3841 tagccgaagc agaagtcgca actggagatg gccacgtccg cgccggcctc gcgcagcacg
     3901 tccagcagcc cctcctcctc ggcctgccgg tggatgacct ctgacgtcgg cacgacgttg
     3961 aggcgcacgc cgggcgcgac gcggcggccg cgcaggatct gggcggcggc cctgatgtcc
     4021 tcgatgcggc cgctggcgca ggagccgatg aacgcccgcg tgatgggcgt gcccgcggcg
     4081 tcctcgacac cgagcgtgtt ggcggcccgc gccgacccgg gcaggacgat ccggggctgg
     4141 agcgcgtcga ggtcgatgtg gtgggtggcg gtgtaggacg cgtccggatc gctggtcagc
     4201 ggctcgaact ccttgcgcgc gacgctccgg gcgtactcca gcgccaggtc gtcggggttg
     4261 aagatggcgg agaccgcgcc cgtgaacatg gccatcccgc acaggccctg gcggcggccg
     4321 atgctcatcg cctccgcgcc cggtccggtg aactccatga cctggtgggc gcaaccgtcc
     4381 gcgccgaccc gggagatgat ctcgtggatg aggtcgcggt tgtcgacgcc gggcgcgaag
     4441 gcgccggtga ggtggaagcg cgtcgaggcg ggcacgtcca ggaagatctg accgctgacc
     4501 caggcctcca gcaggtcgcg gcgcacgccc cagcccaggg ctccgaacgc gccgaggccg
     4561 ctgatgtggc cgtcgaaatg gatgaggaac gttccaggca gggcgtagcc catctcggcc
     4621 gccacctggt gaccgatgcc gcggccctcg tgcacggtga tcccgtagaa gtcgccccag
     4681 cggcgggtga cgtcgtgcac ctcccgttcc cgcccgtcgc ggttggacag catgtggtcg
     4741 atgaagatga ggtagcgctc gggatcgtcg accgtggtga tgccgaagtc gtcgtgcatc
     4801 tgcttgaaca tcaggtcggt gtagcccggg aagtcgtagg ccaccatgta gtccggccgg
     4861 aaggcgtggt tctgcccggc gcgggcggtg ccgagcccgg ccgcgcgggc gaggatcttc
     4921 tcggtgatgg tctgcgccgt ggcggtccgc gcggtcgccg cggtgcgggt ggcgtcgtcg
     4981 aatcgcgtca tcgtgccccc tcaggctccg gccacgggca ggtacttctc ctgcgccctc
     5041 tcgatgtcgg cgaagccgat cagatcgacg aagtcgtggt gggggagcgc gtccgcgctg
     5101 acctcggcgg gcggctcacc ggactccagc cgccggagcg tctcgcgcat cgcccccgtc
     5161 gccgacagca ggatctgcgt gggcagcagg gcgagcctca caccgacctc gcgcagcacc
     5221 tcgggcgtga agtcccgttc gacccaggtg gacgcggtga gcgtggccat cagcgggacg
     5281 ccggcgcgct cgcggcaccg tgcccagtcg tccacgcccg cgaaggtctt ggtgaccggc
     5341 atgatcatct cggccccggc ggcggcgtag gcctccgctc ggccgagtgc ctcctcgccc
     5401 gcggtgtccg tccgggcgat caccaccagc cggtcgccga ccgcgtcgcg gacggcacgg
     5461 atcttcccgg cggcctcctc cagggagatc accggaaccg ggtcgccgac gcagatcggg
     5521 cagcgcttcg gtgactcctg gtcctccatg aagaccgcgg ccacccccgc ggcggcgaac
     5581 cggcgggccg tccgcgcggc gttcacagcg ttgccgtagc cggtgtcgat gtcggcgatg
     5641 accggcaggt cactggtctc gatgatccgc cggaccgcgt cgaggttctc cgacatcgtg
     5701 tacagctccg cgtcgggcag gccgagtgcg gcggagatcg cgaagccgga ggcgcacagg
     5761 gccgggaagc cggcctgctc ggccaggcgc gcgctcagcc cgtcccacac gccgggggcg
     5821 aagaccacgt cgtcgtccat cagttcacgg agccgcgtcg ctgtcatgcg ctcacccttt
     5881 ccggatagtg aaacggattt cggagaagac attaggtatg agcctcctgc ctgtcaatga
     5941 gtccttcctg ccgggatctc gtcctatgaa ggctggtcaa ggtgattcgc cgcggatgtg
     6001 ttgactgcgc cgaaccctca tgtgatagtc cgtaatccgg aacatcttcc gagagcttcg
     6061 catcggctgg aggatcacat ggacacgttc tggccggggg tcggcgccaa ctggggacgc
     6121 tggggcgagc gcgacgagcg cggaacgctg aacctcgtcg gcccggagaa gatccgttcg
     6181 gccgcggcga gtgtgcggag cggtcgcgcg atctctcttg ccagaacgct cgacgaccgc
     6241 ctgccggcct actatcccgc agccggggag agcgccttcg agcaccggat gatcacctgg
     6301 tggggctcca acgcgggcgg cgacgtccag gccgcgagcg accgggtgag ttccgagtgc
     6361 cacggcctgg agatcacgca catggacgcg ctgagccata tcgggcaccg gggccgcggt
     6421 tacggaggac gcgacttcgc ctcgatggcc ggtccggacg gcgtggcgga gtgcgacatc
     6481 acggccgccg cccccgtggt gacacgcgcc gttctggccg acatcccgcg gctgcgcggc
     6541 gtggaccacc tcccggccgg caccgccgtc ggccacgacg aactcgccga ggccgcgccc
     6601 ggactcgggc cgggcgacgc cctcgtcgtc cgcaccggcc gttggaacgc gccaagtccc
     6661 gcgcccgatc ccggcgaccg gtacgggcac ctgtcgggcc tgcatcccga cgccctgcgc
     6721 ttcgtggcgg accgggacgc cgccgtggtc ggaacggacg gccccgggga cgccttcccc
     6781 gtcgcggtcg agcactgccg gctcccggtg cacgtgctcg cgctggtgta cctgggcgtc
     6841 caccttgtgc acagcatggt gctcgacgag ctcgccgccg cgctcgccgc cgaggaccgc
     6901 accgatttca tgctcgtcct cggaccgctg gtggtccccg gcgcgacggg ctccccgatc
     6961 acccccgtgg ccgtcctctg acaagcatcc gatgcgcctc gtgagacccc gcgacaggcc
     7021 cccggcccgg ccccggcgac gcgtagtgcg aaccgggggc ctcgcaccgc aagggccggt
     7081 gcatcacgtc cgaaccagag cccgtggacg cagcctgtcg aggaggcatg tgtccgaggc
     7141 gactcccgct accgcgaccg agggatgaac atggagcgca ccgttgactt cctgtgcata
     7201 ggcggcggcc tcggcggcct ggccggcgcc gtccgcgcac acgacctggg cgcggacgtc
     7261 ctggtcgtgg aacgatccga catggtgggc ggcgtggccg cctacagcgg cggcttcgtc
     7321 tgggtcggtg ccaacgacct caccggctcg gccgactcgc tcgccgcgac cgaggcgtac
     7381 ctggaccacg tgcaggggca gggccgtccg gtcgatcgcc cggcccggcg cgcctacctg
     7441 gaactggccg tcgaggcgac tcggtggtac cgcgaggccg gcgtcccgtt cgaggtgatc
     7501 cggaactgcc ccgacctcta ccacccggcc cccggctcga cccccgaggg gcggctgctg
     7561 gagtgcgtcg tacccggcgc cacgctcggc ccctggcgcg acaggctgcg tccgagtccc
     7621 tattacgtga ccgggatacc ccggaacgac ctttactccg agagttcgac ggacccgaac
     7681 gaagtcgacc gctggcaacg gcgcggcgcc gaccgggacc tgctgacgca cggctccggg
     7741 ctgtcggcgg ccttcatccg cgcggcgctc accgaacgcg gcatcgactg cctgccccgg
     7801 caccgggcga tccggctgct catggacggc gacgcggtca agggcgccgt cctcgacgga
     7861 cccgacgggc gcgtgacggt ccgggcgagc cgcggcgtgc tgatcgccgc aggcggatac
     7921 ggccacgccc ccgacgcctg ggaactggag gacgtcccgg cgctcgtcga gtcggccccg
     7981 cccgtcgtcg agggtgacaa cctgctgctc gcccagcagg cgggcgccgc gaccgtccgc
     8041 ggtgccgacc cgttcttcag cgtcggattc cactttcccg gcgagaccca cccgggcggc
     8101 gacctgcccc tctgccggcc cctgctggag catctcgggc taccgcactc gatgatcgtc
     8161 aaccgggacg ggcgccggtt cggcgacgag gcctactacg gtgcactgat cggggccctg
     8221 cggcgcttcg acggccggag caggcagtgg tccaactacc cctgctggtt cctcgccgac
     8281 gaggagttcc gccggcgcta ccggctgggg aggtacgccc ccggcgaacc ctggccggac
     8341 gagatcgtcc gcgcggactc gccggaggac ctcgcgcggg ccacgggcat tgatcccgac
     8401 gggctggccg cgacgttcgc gtccttcaac aagggcgcgg aacgcggtga ggacccggag
     8461 ttccaccgcg gctccctgcc cttcgtccgc cgcgcctatg gcgaccccct gcacgagccg
     8521 aatccgaacc tcggaccgct cgccacgccg ccgttctacg ctcttcccct ctcgatcgtc
     8581 ggcttcggga tgggcacgct cggactctgg atcgacggca gcgcgcgcgt acgacggcgc
     8641 gacggcgcgg tggtgcccgg gctctacgcc accggcaacg ccgccgccac gaaggaactc
     8701 aaggggtatg tcaccggcct cgccaacgcg cgcaactaca cctacgccta cgccgccgcc
     8761 acccacgccc tgggctcgtc cgcggccctg gatcgataga atcggaatat ggaccggatt
     8821 acggaacgcg cagggaggtc ggatgacccc cgatgaccgg gagggcgtcg accccaagaa
     8881 cgtcgtggtg tcggtgctca aggcctgcca gctcatggag tgcttcaccc gggagaaacc
     8941 gaacctgaca ctcgccgacc tgaccgtggc caccggcatg aacaagacca cggtccaccg
     9001 gctggccgcg acgctgatcc aggccggctg gctgacccgc ggcgagggcg ccacctaccg
     9061 gctcaccatg cgccccttcc gggtcggcgc cgtcgcgctc gccgaactca gcctccgcag
     9121 cgaagcggtc ccgtttctgc gcggcctggc cgaccggttc ggtgacaccg cctacctcat
     9181 gatccccggt cccgacggcg ccgtctgcgt cgacatcttc cagggcaaca acccggtccg
     9241 ggtcaacacg gtcggcgtcg ggacggtgct gccgtaccac accgcggcgg gcccggtggt
     9301 gatgctcgcc ttctcggacg aactgcggga acgctggctc gcccggacgc tcgaccagta
     9361 cacctcgaac acgctcgtcg acgcgccgtc cctcaaggac cggctcgaca agatcgtccg
     9421 cgacggctac gcgatcagcg aggaggacta cctctacggc gtcggcgccg tcgccgcccc
     9481 cgtcttcgac gcgcagggcg aactcgtcgc gaccctgagc ctggggggcg tcaacgacgg
     9541 cttccgcggc agccgtctcc tggagatcat cgaggccgtc ctgtcctcct cccgcgaact
     9601 gtccagccgt ctgggccact gaaggacaca ccgcactctc aacccgtcgc tcaccccccg
     9661 atccccgcat gcatgaggaa aggaagacca tggacgcccg ctacaccgcc gtcgccaccg
     9721 ccaacggccg ggacggccga gccgtgacct ctgatggacg cctggacgtc tccctggccc
     9781 cgccccgtga gctgggcggc agcggcgagg gcaccaaccc cgagcaactc ttcgcggccg
     9841 gctacgcggc ctgtttcgcc agtgcgctca gcgcggtcgc gcgggcctcc cgtcacgacg
     9901 tccgtgacgt ctcggtgacc gccgaggtcg gcctccacgg cgacaccgag aagggcttcg
     9961 gcctcggtgt ggtcctccgc gtagaactgc cggacgacct cgccgacggc gaacaactcg
    10021 tagagcgcgc ccaccagatc tgcccctact ccaacgcgac ccgaggcaac atcccggtcg
    10081 acctggtgat cgagtagagc gaccacggat cgggcgtcgg ccccggcggc cggagtcccc
    10141 caggacgact tggatgccgc cttccgtcgg tgcgtcgggc gacccatttc gcggtggtcc
    10201 gccgacatag gattgggcat tcggggcgga cggggaaagg gcgttctttg gggctgggcg
    10261 gcggtgccgg ggagatcggt gagtttccgg tggcattgcg ggagaaattc cggccggtcc
    10321 ggatcgaggc ggtcggcgcc gagtcggtcg tccacctggc cgagccgctc ggcggcgggt
    10381 gcgcgggcgc cgacctggtc gtcgtcaagg tgcaccggga ctcccggctg gagaccgacc
    10441 agatcgtggt gctggggcgg ctggaggcga cccggcacgt cccccggatc catgattacg
    10501 ggaacctcgc cgtggacggc gccgaggtcg gctggatggc gcaggagtac tgcgcgcacg
    10561 gctcgctgga tgcgctgatc cgggacgccc tcccgctcgg cgacgcggaa ctgcgggcga
    10621 tcgtcgccga actcgccgaa tgcctgaact tctggctggg cgccatgggg ttgacgcaca
    10681 cggacgtgaa accggccaac atcctggtgc ggtcgcggaa cccctatgaa ctgctgatcg
    10741 gcgacttcgg aggcatagcc cgcaatgtcc gggtccatgg gcgggatccg ggcgggctgc
    10801 tgaccacctg ggcctacgcc gcgcccgaga ccgcgcgggg ccgccgcggc gctcccgccg
    10861 cctggtg
//
