LOCUS       NZ_BMRD01000005        12857 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence05, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000005
VERSION     NZ_BMRD01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 553484)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 105933
            Orig. end                         :: 118790
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12857
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12857
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12857
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7857](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7857
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(199..214)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 24.49"
                     /tool="antismash"
     misc_feature    846..863
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.2"
                     /tool="antismash"
     misc_feature    857..874
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.75"
                     /tool="antismash"
     gene            complement(923..1651)
                     /locus_tag="IEZ14_RS14245"
                     /old_locus_tag="GCM10010177_28860"
     CDS             complement(923..1651)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024701.5"
                     /locus_tag="IEZ14_RS14245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28860"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_179831614.1"
                     /transl_table=11
                     /translation="MSKTSAGSREKLIEAGMRLLEERGPEALNARGLAAEIGASTMAVY
                     THFGGMAGLYEAIGREAFARFERHLRQVPDTEDPVTDLLVLGLAYREFAMASPQRYRLM
                     FGIASPGALPSFGHDLTAEGTPATAAEVNDAFALLPRLVRRAMNAGRIRRDDPVLVAGQ
                     IWCMIHGYVLLESTGVFGQEGRGVLHILGPHVINLLVGLGDDRAAAEHSAGQSLLNVEA
                     AAAVAPEPAPRRGRRPRPAQ"
     gene            complement(1740..2594)
                     /locus_tag="IEZ14_RS14250"
                     /old_locus_tag="GCM10010177_28870"
     CDS             complement(1740..2594)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631002.1"
                     /locus_tag="IEZ14_RS14250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28870"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_179831615.1"
                     /transl_table=11
                     /translation="MGLVRSIAVVVAAVAGLGLVGPVASASGPEYYLALGDSLSVGVQA
                     GAGRTDQGYTDRLHAALKARNPRLRLVKLGCPGETTTTMLKGGICRYAAGSQTGAAEAF
                     LKAHAGHVRYVTLDIGANDTNGCLPGGGVDAGCALQGVGSLIVNLPQITARLRLAGGGS
                     PVYAAMNYYNPGLASWVSGGRAAALASIPVIDTFNAYEHGVYLAAGFRVADVNGAFAGH
                     DLTEVPAPPYGTVPRAVARICAWTYQCSDDDGHATAAGYQEIADAFLKVVPTMSTERAP
                     GTA"
     gene            complement(2837..3865)
                     /locus_tag="IEZ14_RS14255"
                     /old_locus_tag="GCM10010177_28880"
     CDS             complement(2837..3865)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012742.5"
                     /locus_tag="IEZ14_RS14255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28880"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_179831616.1"
                     /transl_table=11
                     /translation="MSVTLKDVAAAAGVSRSTASRALSGSSLIAAETRALVEDAARRLG
                     YRPNRAASSLRSRQSHLIGLVVGNLVNASFHTVAEVVQRRAAAEDYQMMLYITDADPRR
                     EETILKTLGEHGVDGLILIGTGSNAAASNALLASGTAVVNLIRGAPESAAPTVLAADRD
                     GAHEATRHLLDLGHRHIGYIGGPSDTNSGQERFAGYEQALRERGLEVDERLVERGPFEP
                     AFGSDATDRLLRRAPETTALLAANHEAVFGVLPALVAAGVSVPDDLSLVCYEDISWLRW
                     WHPPVTVVDNGARELGELAMDLLLQQLERGRGNVPPARNGRTYRVGAQLVQRGSCAPPR
                     AR"
     gene            4039..5004
                     /locus_tag="IEZ14_RS14260"
                     /old_locus_tag="GCM10010177_28890"
     CDS             4039..5004
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012904.5"
                     /locus_tag="IEZ14_RS14260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28890"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_179831617.1"
                     /transl_table=11
                     /translation="MDRDDVSWHGYWPAAPTPFAADGALDEDAWRALLRLYLRQGVHGV
                     LVNGSTGEWFGQSPAERRRVAEIAVAEVAGRVPVVVGVTAYTPAEAIGLARHAADAGAD
                     GVLATPPPYVHPGPEELLAFFAEVSAATDLPFMVYNWPRGVAVDIGDHPDLVRRLADLP
                     HVAAIKDSTGDWLRMLATVEAVADRVRVFGSFLHRRGLAVLLELGGDGNIDGGGLGAPF
                     AVPYYEAVARRDGAAARTWADRYTGLSGRLIRPDYSGVFGSPIAQLKGAMSLLGQPGGH
                     VRPPLRPVTGVALEAIGAVLEESGLTKALARERDAAGARP"
     gene            5001..5258
                     /locus_tag="IEZ14_RS14265"
                     /old_locus_tag="GCM10010177_28900"
     CDS             5001..5258
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024900.5"
                     /locus_tag="IEZ14_RS14265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28900"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_179831618.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.4e-23, bitscore: 77.9,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTGTRITIDGEPVDAAAGASLTAVFVAAGRWRLRDNPVSGEPRG
                     PFCGMGVCFECEVTVDGRPGVRACLTRVLPGMAIETGTVR"
     gene            5255..6682
                     /locus_tag="IEZ14_RS14270"
                     /old_locus_tag="GCM10010177_28910"
     CDS             5255..6682
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF019604.5"
                     /locus_tag="IEZ14_RS14270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28910"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_179831619.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.5e-29, bitscore:
                     97.5, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 3.3e-08, bitscore: 28.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.7e-09, bitscore:
                     32.0, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTASRDVLVVGGGPAGLSAAARLAAAGLGVTLVDEQPELGGQYYR
                     RPSPAVLREHGDHRPEGGRLIAHVRSLGVDCLTGHLVWGVDDDGRTLLATGPDGTPVRL
                     RARHVIVATGAYERTHPFPGWQLPGVTTPGFAQHLAAADGTPPGRRVLVAGSGPFLLPV
                     ACSLLAAGADVVGVAEAGHPYRPSLRAAPALRHPARLRELAGYVARLARARVPLWQGRV
                     VNGAAGGDRVTSVTLAATVDPGRPLRTLDVDALCVGYGFRPQTELARLLGCEISADAVT
                     GDAEPVTDAFGRTSRPGVYVVGEAAGVGGVHRARVRGAMAAAHILLREGSPAAPGPREI
                     ARLAAQGRALTRFTAMSDALYPSPARLQEVLAATLDDDTIVCRCEAVTAAAVRSAAAER
                     AGGDLNAAKSWSRAGMGPCQGRFCGFAVAGLVRDAAEAPPAGGARCGVPVFPARQPVRP
                     VPVETLLALGEQDGGDG"
     gene            6679..7857
                     /locus_tag="IEZ14_RS14275"
                     /old_locus_tag="GCM10010177_28920"
     CDS             6679..7857
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013434.5"
                     /locus_tag="IEZ14_RS14275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28920"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_179831620.1"
                     /sec_met_domain="DAO (E-value: 1.2e-46, bitscore: 155.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAQTADVVVIGAGVLGGAAAFHLALGGHRVIVLDRGAPGREGSG
                     TTAGNLHVQAVHTRRPGQEVPLDSARFLPLQRAASDRWNSVEAELEASVELRRSGGFTI
                     AETPEQRAELIAKHAWEERAGIRTEVLDGDGARAALPLLGPTVTAATWCPLDGYANPLL
                     TTPAYLAAAVRHGARLHPFCPVRAVERSGSGWRVTAGDLTVEAPHVVDVAGPWLGEIAA
                     LAGIGLRMTPVAIQMHVTVRLPETLRYLVQHIGEGLSVKQVTAGNLLIGGGWPALRLDP
                     DGRSPVSLSSVLGNVAQAARVLPFVRDLRLLRTWAGPLAATPDEMPVIGEVPGAPGFLV
                     AGGTYAFTFAPLWGETLRCLVEGKPAPVDVRDLGPDRLLEPAAPAAPYEEAR"
     gene            7854..9182
                     /locus_tag="IEZ14_RS14280"
                     /old_locus_tag="GCM10010177_28930"
     CDS             7854..9182
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014081.5"
                     /locus_tag="IEZ14_RS14280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28930"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_179831621.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.3e-37, bitscore:
                     124.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREETAVPTTTTARRPVPREGTLWLTGGHVVDVLGGTVRRNANVA
                     VAAGRIEKITTEAPEPGAQRVDLGGRYLLPGLISVHTHLSVVYPFSATDEAESPGLTAM
                     RALSRAQDALHAGVTSIRCVHEQNRADLLVRTGVEQGWVDAPRIIGAGRAVSTTGGHGH
                     GSACSYADGHDGFLRAARTELAAGADLIKVFITGGIAHEGESFTGAQMTLDEMRGVVRA
                     AQEHGTYVTAHAGSGSAIREALAAGVRGYEHAYELDDATAKEMAERRVFLTPTLCVTRC
                     PDWMAEHSFSEWQIQRALEVGPGHLASIRRAVAAGIADPRDPGAPGITMLAGTDYPPGE
                     PIEDTVVAVREMEFLTEAGLSPEQALRAGTSDAARLVGLHGKAGAVEEGLLADLIVTDR
                     DPLSDLSALRRIPFVMQGGRVVRDDLPAAPAGRAAADAPGGTL"
     gene            9179..11233
                     /locus_tag="IEZ14_RS14285"
                     /old_locus_tag="GCM10010177_28940"
     CDS             9179..11233
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014071.5"
                     /locus_tag="IEZ14_RS14285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_28940"
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="WP_179831622.1"
                     /transl_table=11
                     /translation="MTSSPRTRLAVDIGGTFVDAIAYDEGTGELRLHKAPTTPDAPARG
                     VLDAVGGLAGRLDDVEAFVHGTTLGLNAILQRRGADVGIITNAGFRDLLEIARANVPGQ
                     HMYDFAYAPPPPLVPRRHRTGVPGRIDAQGAEVEPLDEDAVREAGRILVEEHGLRSLAV
                     CFLHSYAEPAHERRAAQILRDAHPDVSISVSTDISREYREYERTSTATLDAYIRPVLSD
                     YIGALEGRLGDAGLTRPLHIMRSGGGAMTAELARRAPLMTVLSGPAGGVVGASFLAREL
                     GRDKLISFDVGGTSVDCCVIEDGSPGEVHEAAIDGFPLLIPIFDIRTVGAGGGSIAWMD
                     HGLLKVGPQSAGAVPGPVAYGAGGTEPTVTDAALVLGYLDPAAFLGGDMSIDADAARAA
                     VADRLARPLGVGVTEAAASVLRVLLARTVGALREITVERALDPREFALLAFGGAGPLLG
                     PMLAREMGITETVVPQVPAAFSAFGMLMSDLEYEFATTVLKPLSDATLAELEPAFADLE
                     NQGEEVLSLQGVKPEDRTLVRRLDVRYHGQEHTLAIDLRPGDDAAAILDRFHELHRARY
                     GHAMPDGGQILTLRVRAVGVLPKPGLRRLSGSGSGTPDPAGSRPAFDVATGETVPFPVY
                     ERQALAPGHVVPGPAIVEEGTSTTVIFSDQRLTVDVHGHLLVSSPETAQ"
     misc_feature    complement(11791..11804)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     misc_feature    complement(11915..11930)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.33"
                     /tool="antismash"
ORIGIN
        1 cgccgcagcc gctcgcccgg ctccaccccg agatcctcca ccagtcggtg gcgcaggtgc
       61 gagtacgcgg ccagcgcgtc ggcctggcgg ccgctcccgt acagcgcgcg catgagcagc
      121 gaggtcaggg actcgctgtg cgggtgcttg ggcatcaggg cgtgcagctc accgacggcg
      181 tccacgaacc tgccgagctg gaactggcat tcgatcttct cctgcaggag ggagagcctg
      241 cgctcctcca gccgcagacg ttccgcctcc gcgaacggcc ccggaagccc ggccagcggc
      301 tcgccctgga acagcgcgag ggcatcgagg caggcgctcg ccgcggccgc cagatcgccg
      361 gacctcctgg cggcggccgc ctcgtccgcg gtctcgtcga gccgcatcag gtcgaccgcc
      421 gtcccccggc tgacgaagcg gtagccgccc cgctcccggg cgatcaccga ctcctgcgcg
      481 tgctcgccga gcgtgcccag gcagcggcgc agccggtgga cgtacaccgg cacgatcttg
      541 cttcccgagc ccggcggttc gacgccccag acgccgtcga gcagctcccg ctggctgacg
      601 gtcatgtccg ggcgcagcag cagggcggcc aggagggcct gctgcctgag ggggccgagg
      661 tcgagccggg tcgccccgcg ccacgcccgg accgggccca gcacggtgaa gtcgagcagg
      721 cggggggccg ggtccggcgt cgtgtccgag gtcatcgcca tcgccatcat cagaccgatc
      781 tcttcgaact gctgatcaag gacgatccgc gcgtacaggg gagcgcaacg gaccttggca
      841 taactttgta atgggtaggt tattactatg tgatggcccg gtgcggcgat tggttcggac
      901 catcggcgtg gcgatcgtca cgtcactgtg cggggcgcgg ccgccggccc cggcggggcg
      961 cgggctcggg tgcgacggcc gcggccgcct cgacgttcag cagcgactgc ccggcggagt
     1021 gctcggcggc cgcgcggtcg tccccgagcc ccaccagcag attgatcacg tgcgggccca
     1081 ggatgtgcag gacgccgcgg ccctcctggc cgaacacccc cgtgctctcc agcagcacgt
     1141 agccgtggat catgcaccag atctgcccgg ccaccaggac cgggtcgtcc cggcggatcc
     1201 gcccggcgtt catggcgcgc cgcaccagcc gcggcagcag cgcgaaggcg tcgttcacct
     1261 cggcggcggt cgccggcgtg ccctcggcgg tgaggtcgtg cccgaacgat ggaagcgccc
     1321 ccggcgaggc gatcccgaac atcagccggt agcgctgcgg gctggccatc gcgaactccc
     1381 ggtaggccag ccccaggacc agcaggtcgg tcaccgggtc ctcggtgtcg ggcacctgcc
     1441 gcaggtgccg ctcgaagcgc gcgaacgcct cccgcccgat cgcctcgtac agcccggcca
     1501 tcccgccgaa gtgggtgtag acggccatgg tggacgcccc gatctcggcg gcgagccccc
     1561 gcgcgttcag cgcctcggga ccccgttcct ccagcagccg catcccggct tcgatcagct
     1621 tctcgcggga ccccgccgac gtcttggaca tgacaatgtt atagcgcgcc cagaagggtc
     1681 cgctatcacc tgaacggcca tgtctttctg ttgcgacggt ggcgacgccc tccgcggggt
     1741 caggcggtac cgggggcccg ctcggtggac atggtcggca cgaccttgag gaaggcatcg
     1801 gcgatctcct ggtaccccgc cgccgtcgcg tggccgtcgt cgtcgctgca ctggtacgtc
     1861 cacgcgcaga tccgtgccac ggcccggggg acggtgccgt acggaggggc cggcacctcg
     1921 gtgaggtcgt gcccggcaaa ggcgccgttg acgtccgcga ccctgaaccc ggcggccagg
     1981 tagacgccgt gctcgtaggc gttgaaggtg tcgatgaccg gaatcgaggc gagggcggcg
     2041 gccctgccgc ccgacaccca ggacgccagg ccggggttgt agtagttcat ggcggcgtag
     2101 acgggggagc cgcctcccgc cagccgcagc cgcgcggtga tctggggcag attgacgatc
     2161 agcgagccca cgccctggag cgcgcagccc gcgtcgacgc caccgccggg caggcacccg
     2221 ttggtgtcgt tggccccgat gtccagagtc acgtaccgga cgtggcccgc gtgcgccttc
     2281 aggaaggctt cggcggcgcc cgtctgggag ccggccgcgt accggcagat gccgcccttg
     2341 agcatcgtcg tggtggtctc cccggggcag ccgagcttca ccagtctcaa ccggggattc
     2401 cgcgccttca acgcggcatg gagccggtcg gtgtaaccct gatcggtcct ccccgcaccg
     2461 gcctgcacgc ccacggacaa cgagtcgccg agcgccaggt agtactccgg gccgctcgcc
     2521 gaggcgaccg gccccaccag ccccagcccc gccacggcgg cgaccacgac ggcgatcgat
     2581 ctgaccagcc ccatccccgc tccagcgacc tcggcacaag cgcaccccac ccggcacgca
     2641 cgcaacagac ggccaagcta acaaacatcg ctgtctattt aaagggcccc ggcatcgtcg
     2701 gtgccgagcc ggcggggtcg acatgagagc ccaccggggc tccgtccgaa tgcccgggac
     2761 gaattccgac accaggcgca tccatttcga ctgacgccgg gccccgcgtg tcacgtcgcg
     2821 cgggctgtct ggcgggtcag cgagctcggg gtggggcgca ggagcctcgc tggacgagct
     2881 gggctcctac gcggtaggtg cggccgtttc gggcgggggg cacgttgccc cggccgcgtt
     2941 ccagctgctg gaggagcagg tccatggcga gttcgcccag ttcccgggcg ccgttgtcga
     3001 cgacggtcac gggtgggtgc caccagcgca gccagctgat gtcctcgtag cagacgaggg
     3061 agaggtcgtc ggggacgctg accccggcgg ccacgagggc gggcaggacc ccgaagacgg
     3121 cctcgtggtt ggccgccagc agggcggtcg tctccggggc gcggcgcagc agccggtcgg
     3181 tcgcgtcgga gccgaacgcc ggctcgaacg ggccgcgctc gaccagccgc tcgtcgacct
     3241 ccaggccgcg ctcgcgcagg gcctgctcgt atcccgcgaa gcgctcctgc ccggagttgg
     3301 tgtccgacgg cccgccgatg tagccgatgt ggcggtggcc gaggtccagc aggtgccggg
     3361 tcgcctcgtg ggcgccgtcg cggtcggccg cgagcacggt cggggcggcg ctctcgggcg
     3421 cgccgcggat gaggttgacc acggcggtgc cgctcgccag cagggcgttg gaggcggcgg
     3481 cgttggaacc ggtgccgatc aggatgaggc cgtccacgcc gtgctcgccg agcgtcttga
     3541 ggatggtctc ctcgcggcgc gggtcggcgt cggtgatgta gagcatcatc tggtagtcct
     3601 cggccgcggc gcggcgctgg acgacctcgg cgacggtgtg gaacgaggcg ttcaccaggt
     3661 tgccgacgac gagcccgatc aggtgcgact gccgggaccg cagcgaactg gccgcgcggt
     3721 tcggccggta gcccagccgc cgggcggcgt cctcgacgag ggcgcgcgtc tcggcggcga
     3781 tcaaggacga gccgctcagc gcccgggagg cggtgctgcg ggacacgccg gcggcggcgg
     3841 cgacgtcctt cagggtgacg ctcatcgtgc tccgcttctg caatcgatcc cactggcggg
     3901 ccaatcatgc cacaggacga cgggggaggg cagggcaaca aaaaggaaga agacgactct
     3961 tgacacccga gttggctctg ccgcaacctt gtgcaaccga tcccagcggg atgagctcta
     4021 cagactcggg gaaagatctt ggatcgcgac gacgtctcat ggcacggcta ctggccggcg
     4081 gcgcccacgc cgttcgccgc ggatggcgcg ctggacgagg acgcctggcg tgcgctgctg
     4141 cggctgtacc tgcggcaggg cgtgcacggc gtgctggtca acggcagcac cggcgagtgg
     4201 ttcggccaga gcccggccga gcgccggcgg gtcgccgaga tcgccgtcgc cgaggtggcc
     4261 ggccgggttc cggtggtcgt cggcgtcacc gcctacaccc cggccgaggc gatcgggctg
     4321 gccaggcacg ccgccgacgc gggggccgac ggcgtgctcg ccaccccgcc gccctacgtc
     4381 cacccggggc cggaggagct tctcgccttc ttcgccgagg tcagtgcggc cacggacctg
     4441 ccgttcatgg tctacaactg gccgcgcggc gtcgccgtcg acatcggcga ccacccggac
     4501 ctggtccggc ggctggcgga cctgccccac gtcgccgcga tcaaggacag caccggcgac
     4561 tggctgcgga tgctcgcgac ggtcgaggcg gtcgccgacc gggtccgcgt cttcggcagc
     4621 ttcctgcacc gccgcggcct ggccgtcctg ctggagctcg gcggcgacgg caacatcgac
     4681 gggggcgggc tcggcgcccc gttcgccgtc ccgtactacg aagcggtcgc gcgccgcgac
     4741 ggcgccgccg cccgcacctg ggcggaccgc tacaccggcc tgtcgggacg gctcatccgc
     4801 cccgactaca gcggcgtgtt cggctcgccg atcgcgcagc tcaagggggc catgtccctg
     4861 ctcgggcagc cgggcgggca cgtccgcccc ccgctgcggc cggtcaccgg cgtcgcgctg
     4921 gaggcgatcg gcgcggtcct ggaggagtcc ggcctgacga aggccctcgc tcgggagcgg
     4981 gacgcggccg gggcgcggcc gtgaccaccg gcacccggat caccatcgac ggggaacccg
     5041 tggacgccgc cgcgggcgcg tccctgacgg ccgtgttcgt cgccgccggg cgctggcggc
     5101 tgcgcgacaa cccggtcagc ggggagccgc gcgggccgtt ctgcgggatg ggcgtgtgct
     5161 tcgagtgcga ggtcaccgtc gacggacggc ccggtgtgcg ggcgtgcctg accagggtcc
     5221 tgcccggcat ggcgatcgag acggggacgg tccgatgacc gcctcgcgcg acgtgctggt
     5281 cgtgggcggc ggcccggcgg ggctgtcggc cgcggcccgg ctggccgccg ccgggctcgg
     5341 cgtgaccctc gtcgacgagc agcccgagct cggcggccag tactaccgcc gcccgtcgcc
     5401 cgccgtgctg cgcgagcacg gcgatcaccg cccggagggc gggcggctga tcgcgcacgt
     5461 gcggagcctc ggcgtcgact gcctgacggg ccacctggtc tggggagtcg acgacgacgg
     5521 acggacgctc ctcgccaccg gcccggacgg gaccccggtc cggctgcgcg cacgccacgt
     5581 gatcgtggcc accggcgcct acgaacggac ccacccgttc cccggctggc agcttcccgg
     5641 cgtcacgacg cccgggttcg cccagcacct ggccgccgcc gacggcaccc cgccgggcag
     5701 gcgggtgctg gtcgccgggt cgggcccctt cctgctgccc gtcgcctgct cgctgctggc
     5761 cgccggcgcc gacgtggtcg gcgtggcgga ggccgggcac ccctaccggc cgtcgctgcg
     5821 ggccgccccg gcgctgcggc atcccgcccg gctccgcgag ctggcgggat acgtggcgcg
     5881 cctggcccgt gcgcgcgttc cgctctggca ggggcgcgtg gtgaacgggg ccgcgggcgg
     5941 tgaccgcgtc acgtcggtca cgctcgccgc caccgttgat ccgggacggc cgttgcggac
     6001 gctggacgtc gacgccctct gcgtcggcta cggcttccgg ccgcagaccg agctggcccg
     6061 cctgctcggc tgcgagatca gcgccgacgc cgtgaccggc gacgccgaac ccgtcaccga
     6121 cgccttcggc cgcacgagcc gccccggcgt gtacgtggtc ggtgaggcgg cgggcgtcgg
     6181 cggcgtccac cgggcccgcg tccgcggggc gatggccgcc gcgcacatcc tcctccgaga
     6241 ggggagcccc gccgccccgg gcccccggga gatcgcccgg ctcgccgcgc agggccgcgc
     6301 gctcacccgg ttcacggcga tgagcgacgc cctgtacccg agccctgccc ggttgcagga
     6361 ggtgctcgcg gccaccctgg acgacgacac gatcgtgtgc cgctgcgagg cggtgaccgc
     6421 cgccgccgtg cgctccgccg ccgcggagcg ggccggtggc gatctgaacg ccgcgaagtc
     6481 ctggtcgcgc gccgggatgg ggccctgcca gggccgcttc tgcgggttcg ccgtcgccgg
     6541 tctcgtccgc gacgccgcgg aggccccgcc cgcgggcggg gcgcggtgcg gcgtaccggt
     6601 cttcccggcg cgccagcccg tccgccccgt gcccgtcgag acgctgctcg cgctcggcga
     6661 gcaggacggg ggcgacggat gagcgcgcag accgccgacg tggtcgtgat cggcgccggg
     6721 gtgctgggcg gcgcggccgc cttccatctc gccctgggcg ggcaccgggt gatcgtcctg
     6781 gaccgcggcg ctccgggccg ggagggatcg ggcacaacag cgggtaacct gcacgtccag
     6841 gccgtccaca cccggcggcc ggggcaggag gtcccgctcg acagcgcccg gttcctgcct
     6901 ctccagcgtg ccgcgagcga ccggtggaac tcggtcgagg cggagctgga ggcctcggtg
     6961 gaactgcgcc gcagcggcgg tttcaccatc gccgagacgc ccgagcagcg tgccgagctg
     7021 atcgccaagc acgcgtggga ggaacgggcc gggatccgca ccgaggtcct ggacggcgac
     7081 ggggcccgcg ccgcgctgcc gctgctcggc cccaccgtca ccgcggccac ctggtgtccg
     7141 ctggacgggt acgccaaccc gctgctgacc acccccgcct acctggcggc ggcggtgcgg
     7201 cacggagccc ggctgcaccc gttctgcccc gtccgcgccg tggaacgttc cggatcgggc
     7261 tggcgggtca ccgccgggga cctcaccgtc gaggccccgc acgtggtcga cgtggccggg
     7321 ccctggctcg gggagatcgc cgcgctggcg gggatcggcc tgcggatgac gcccgtcgcc
     7381 atccagatgc acgtgaccgt gcggctgccc gagacgctgc ggtacctcgt ccagcacatc
     7441 ggcgagggcc tgtcggtcaa gcaggtcacc gcggggaacc tgctgatcgg aggcggctgg
     7501 cccgccctgc ggctggaccc cgacggccgg agcccggtca gcctgtccag cgtcctcggc
     7561 aacgtcgccc aggccgcccg tgtcctgccg ttcgtgcggg acctgcgcct gctgcggacg
     7621 tgggccggcc ccctggccgc caccccggac gagatgccgg tgatcgggga ggtgccgggc
     7681 gcgcccggct tcctggtcgc gggcggaacc tacgccttca cgttcgcccc gctgtggggc
     7741 gagaccctgc gctgcctggt cgaggggaag ccggccccgg tcgacgtccg cgacctcggg
     7801 ccggaccggc tcctggagcc cgccgcgccc gccgcgcctt atgaggaggc ccgatgagag
     7861 aggagacagc ggtgccgaca acgaccaccg cgcggcggcc cgtgccgcgg gaggggaccc
     7921 tctggctgac cggcgggcac gtcgtcgacg tgctcggcgg gacggtccgc cgcaacgcca
     7981 acgtcgcggt cgcggcgggc cggatcgaga agatcaccac cgaggcgccg gagccgggcg
     8041 cccagcgcgt cgatctcggc ggccggtacc tgctgcccgg cctgatctcg gtgcacacgc
     8101 acctgtcggt cgtgtacccg ttctccgcca ccgacgaggc cgagagtccc ggcctgaccg
     8161 cgatgcgagc cctgtcacgg gcgcaggacg cgctgcacgc gggcgtcacg tcgatccgct
     8221 gcgtccacga gcagaaccgc gccgacctgc tcgtccgcac cggcgtcgag cagggctggg
     8281 tggacgcgcc ccgcatcatc ggcgcgggcc gcgccgtctc caccaccggc ggccacggcc
     8341 acggctccgc ctgctcctac gccgacggcc acgacggctt cctgcgcgcg gcgcgcacgg
     8401 agctggccgc gggcgcggac ctgatcaagg tgttcatcac cggcggcatc gcgcacgagg
     8461 gcgagtcgtt caccggcgcc cagatgacgc tggacgagat gcgcggggtg gtgcgggccg
     8521 cgcaggagca cggcacctac gtcaccgcgc acgcggggtc ggggtcggcg atccgggagg
     8581 cgctcgccgc cggggtccgc ggctacgagc acgcctacga gctggacgac gccaccgcga
     8641 aggagatggc cgagcggcgc gtcttcctca cccccaccct gtgcgtcacc cgctgccccg
     8701 actggatggc cgagcactcc ttcagcgagt ggcagatcca gcgcgccctg gaggtcggcc
     8761 ccggccacct cgccagcatc cgccgggcgg tcgccgcggg catcgccgac ccgcgggacc
     8821 cgggcgcacc cgggatcacc atgctcgccg gcacggacta cccgccgggc gagcccatcg
     8881 aggacaccgt cgtcgccgtc cgcgagatgg agttcctcac cgaggccggg ctgagcccgg
     8941 aacaggcgct gcgcgccggc accagcgacg ccgcccggct cgtcggcctg cacggcaagg
     9001 ccggggcggt cgaggagggc ctgctcgccg acctgatcgt caccgaccgc gacccgctgt
     9061 cggacctgtc cgcgctgcgc cgcatcccct tcgtcatgca gggcggacgc gtcgtccgcg
     9121 acgacctgcc cgccgcgccg gccggacggg ccgccgccga cgcccccgga ggcaccctgt
     9181 gacgtcctcg ccccgtaccc ggctcgcggt cgacatcggc ggcacgttcg tcgacgccat
     9241 cgcctacgac gaggggaccg gcgagctgcg gctgcacaag gccccgacca ccccggacgc
     9301 ccccgcgcgg ggtgtcctgg acgccgtcgg cgggctcgcc ggccggctgg acgacgtcga
     9361 ggcgttcgtg cacggcacca cgctcgggct caacgccatc ctgcagcgcc gcggcgccga
     9421 cgtcgggatc atcaccaacg ccgggttccg ggacctgctg gagatcgccc gcgccaatgt
     9481 gcccggccag cacatgtacg acttcgccta cgcgcccccg ccgccgctcg tcccgcgccg
     9541 gcaccggacg ggagtgcccg gccggatcga cgcccagggc gccgaggtgg agccgctcga
     9601 cgaggacgcc gtccgcgagg cgggccggat cctggtcgag gagcacgggc tgcgctccct
     9661 cgccgtctgc ttcctgcact cctacgccga gcccgcgcac gagcggcgcg cggcgcagat
     9721 cctgcgcgac gcccacccgg acgtgtcgat ctcggtgtcg accgacatca gccgggagta
     9781 ccgggagtac gagcggacga gcaccgcgac cctggacgcc tacatccggc ccgtcctcag
     9841 cgactacatc ggggccctgg agggacgcct cggcgacgcc gggctcaccc gcccgctgca
     9901 catcatgcgg tcgggcggcg gcgcgatgac cgccgagctg gcccgccgcg ccccgctgat
     9961 gacggtcctg tcgggtccgg cgggcggcgt ggtcggcgcc tcgttcctgg cccgcgagct
    10021 cggccgggac aagctgatct ccttcgacgt cggcggcacc agcgtggact gctgcgtgat
    10081 cgaggacggc tcccccggcg aggtgcacga ggccgcgatc gacggcttcc cgctgctcat
    10141 cccgatcttc gacatccgca cggtcggcgc gggcggcggc tccatcgcct ggatggacca
    10201 cgggctgctc aaggtcgggc cgcagagcgc cggcgcggtg cccggccccg tcgcctacgg
    10261 ggccggcggc accgaaccga ccgtgaccga cgccgcgctg gtgctcggct acctcgatcc
    10321 ggccgcgttc ctcggcggcg acatgagcat cgacgcggac gcggcccgcg ccgccgtggc
    10381 ggacaggctc gcccgcccgc tcggcgtcgg ggtgaccgag gccgccgcga gcgtcctgcg
    10441 cgtcctgctc gcccggacgg tcggcgcgct ccgcgagatc acggtcgagc gggccctgga
    10501 cccccgcgag ttcgcgctgc tggcgttcgg cggcgccggc ccgctgctcg gcccgatgct
    10561 ggcccgcgag atgggcatca ccgagaccgt cgtcccgcag gtcccggcgg cgttctcggc
    10621 gttcgggatg ctcatgtcgg acctggagta cgagttcgcc accaccgtgc tcaagccgct
    10681 cagcgacgcc accctcgccg agctggaacc ggccttcgcc gatctggaga accagggcga
    10741 ggaggtgctg tcgctccagg gcgtcaagcc ggaggaccgc accctcgtcc gccgcctgga
    10801 cgtccgctac cacggccagg agcacacgct ggccatcgac ctgcggcccg gggacgacgc
    10861 cgccgcgatc ctcgaccggt tccacgagct gcaccgcgcc cgctacgggc acgccatgcc
    10921 ggacggcggg cagatcctca cgctgcgggt acgggcggtc ggcgtcctgc cgaagcccgg
    10981 cctgcgcagg ctctccggca gcggctccgg cacgcccgac cccgccggga gccgccccgc
    11041 cttcgacgtc gccaccggcg agaccgtgcc gttccccgtc tacgagcggc aggccctcgc
    11101 gcccggccac gtggtgccgg ggcccgcgat cgtcgaggag ggcacctcca ccaccgtgat
    11161 cttcagtgac cagcggctca ccgtggacgt ccacggccac ctgctggtga gcagcccgga
    11221 gacggcccag tgagccagac aggagatggt ccgatgagca cgacccggcc cggcggagag
    11281 gcggcggccg cgctggacgg cgcgaccgtc gaggtgatcc gcaactacct ggtctccgtc
    11341 ggcgagcaga tgcgccgcac gctggtgcgg gccgcgttca accccgtgat ctacgaggtc
    11401 ctcgacttcg gcatctcgat ctacgacggg gacctggagc tgatggccga ggccgccggg
    11461 atcacctcgt tcctcggcgc caacgaccac gcgatcgtca agggcgtcga gtacgtgggc
    11521 cgggagaacc tcgcgcccgg cgacgtctac ctgctcaact acccgtactg gagcggcgcg
    11581 cactcctacg acgcgatgct gttcgcgccg gtgttccggg agggccgcga cgagccgtcc
    11641 gcctacctcg ccgtccgcgc ccactggatg gacctcggcg cgaaggcccc gggctacgtc
    11701 ctcgactcca ccgacatgca ccaggaaggg ctgatcttcc ccggcacgag gatcgtgcac
    11761 gggggcgagg tcgtccgcga cgtcgtcgag ctgatccgct tcaactcgcg gctgccggac
    11821 ctcaccgtcg gcgacttcca cgcccaactc gccgccctgc gcaccgggga gaaccggctc
    11881 gcgcaggtgt gggacaagtt cggcggcgag gtcgtggacc gggccgtcaa gcagatcatc
    11941 gagcacggtg agcgcgtcgc gcgcacggcg gtcgccgccc tgccggacgg cacctggacc
    12001 gccgccgact actgcgacga cgacggcatc accgacgacc tgatccgcat ggaggtcagg
    12061 gtcaccatcg acggcgaccg gatggaggtc gacttcaacg gctcggacgg cgccgtgctc
    12121 ggcccggtga accttccgat cggggccacc gagagcctcg ccaagtccac cttcaaggag
    12181 ctgaccacgc cggacgagcc gtccaacgcc ggccactacc gggccctgac cgtcaaggcg
    12241 gacccgggga acttcttcca cgccgtctac ccgtccgcga ccttcaccca gtggagcggc
    12301 atcgtcgcct tcgagctgat ccacaaggcg ctcgcgggcg tcctcgactc gatcccggcc
    12361 tcctccgggg gcgacgagcc cggattcatg gccctcggcc acgacccgcg caccggcgac
    12421 gacttcgtca tcagcaacaa cgagggcatc ggctggggcg cgcggcggga ccacgacggc
    12481 gccaacgcgc agcagcaccc gtcgcagagc gtggtccgca acaccccgat cgaggtgctg
    12541 gagcacaagt cgacggtctt ccacgagcgg ctggagctga tccccgactc cggcggcgcg
    12601 ggccgctacc gcggcggcgt cggcgtcctg cgcgaggtcc gctacctcgc cgacggcgag
    12661 gtgctgtcga tgaagaagaa gagcaagacg cggccgtggg cgctgcacgg cgggctggag
    12721 cccgagccca gcgggatgac gctgtggccc ggaaccgacc gcgagaagcg ggtcggcatg
    12781 taccgcgccg cgatgcgggc cggcgaccgg ttcgtcaacc gcaccgcggg cgggggcggc
    12841 cacggcgacc ccctcga
//
