LOCUS       NZ_BMRD01000001        10396 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence01, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000001
VERSION     NZ_BMRD01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 994668)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 921855
            Orig. end                         :: 932251
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10396
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10396
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10396
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5396](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5396)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(237..252)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.88"
                     /tool="antismash"
     misc_feature    361..375
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(743..1690)
                     /gene="ehuA"
                     /locus_tag="IEZ14_RS04340"
                     /old_locus_tag="GCM10010177_08810"
     CDS             complement(743..1690)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621335.1"
                     /locus_tag="IEZ14_RS04340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08810"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_229809926.1"
                     /transl_table=11
                     /translation="MPPIDTDAVDPGGEADRPPSEGANAAQDPAPGEAAADAGVSTQKG
                     TATKADPAAGLPRGEAPGIRFEKVVKRFGSNVVLRELDFTVAPGERVTLIGPSGSGKTT
                     ILRLLMTLEKLDDGLIWIGDETLWHMDRGGKRVPANQKHLHEMRKQVGMVFQQFNLFPN
                     MNVLRNLTEGPVRVLGVSKDEAVERARGLLDMVGLSDKENAHPTQLSGGQQQRVAIARA
                     LAMQPRVLLLDEVTSALDPELVVGVQDLLRDIARQSDLTLLCVTHEMAFAKDISDRVLM
                     FDQGQIVEEGPPDQIFGEPSEQRTRDFLQAVLAS"
     gene            complement(1677..2330)
                     /gene="ehuD"
                     /locus_tag="IEZ14_RS04345"
                     /old_locus_tag="GCM10010177_08820"
     CDS             complement(1677..2330)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630633.1"
                     /locus_tag="IEZ14_RS04345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08820"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_179833974.1"
                     /transl_table=11
                     /translation="MSWDWHYSGEILPDLLDGLRYTLTATLLGYLIALVLGLVWTLLRR
                     SPSRVFNQVIRWVTEFIRSTPLIPQLYFVFFVLPAWGITIGSLTAGTITLGIHYSTYTA
                     EVYRAGIADVPKGQWEACTALNLPRSRVWLDVVLPQAIRRVIPTLGNYLIAMFKDSPLL
                     LAINVSELLFSAYQVGSKTLQFLEPITMVGLLFVVVSVISSILVRRLERRYATN"
     gene            complement(2330..3043)
                     /gene="ehuC"
                     /locus_tag="IEZ14_RS04350"
                     /old_locus_tag="GCM10010177_08830"
     CDS             complement(2330..3043)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598655.1"
                     /locus_tag="IEZ14_RS04350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08830"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_179833975.1"
                     /transl_table=11
                     /translation="MSEVLDSYPQWLDGAWVTIRLTLLGGALALVLALVFGMAGTSRHA
                     WVRVLNRVYVEFFRGNATLVLLYWFFYALPLIGLRFSTMFAAVLALGLNVGAYGAEVVR
                     GSINAVPKAQYEATIALNFTPFQRMRRVLLPQAFALMLPPFGNLIIELMKGTAIVSLVG
                     LVDLTRVSKNIQGSTGQTVAAFTVVLVLYFVIAQVLQLFVRYAERRVDRMLGRRLPAEP
                     SLGTIAAGAPGAGVG"
     gene            complement(3047..3940)
                     /gene="ehuB"
                     /locus_tag="IEZ14_RS04355"
                     /old_locus_tag="GCM10010177_08840"
     CDS             complement(3047..3940)
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630631.1"
                     /locus_tag="IEZ14_RS04355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08840"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_179833976.1"
                     /transl_table=11
                     /translation="MTSSRRDFLRTAVLLSAAAPLAAAGCSTTDPEKEKTGGGTLQKAK
                     DSGTITVGFANEAPYGYTDKSGKLTGEAPELARAIFKNLGIDEIKGVQVDFGGLIGGLN
                     AKRFDAIAAGMFITPERCASAAFANPEYVAKSAFMVPEGNPKGLKAAEDPGKKGVKVGV
                     LTGAVEAGYAEKTGVKKGDIKTFADQASAYEGLKAGRIDCIWLTRISLADLLTKHKGEG
                     FEVTDAFTPVIDGKEQLGAGAFAFRKADTDLVNAWNGELAKLQQGNKLLPILQPFGFTQ
                     AEMPGPDDTAAKFCQG"
     misc_feature    complement(3978..3992)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.03"
                     /tool="antismash"
     gene            complement(4039..5004)
                     /gene="thpD"
                     /locus_tag="IEZ14_RS04360"
                     /old_locus_tag="GCM10010177_08850"
     CDS             complement(4039..5004)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630630.1"
                     /locus_tag="IEZ14_RS04360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08850"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_179833977.1"
                     /sec_met_domain="PhyH (E-value: 3.5e-35, bitscore: 118.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIIESHPTIDDAYPTRKASEAALLYRQDPVVYGEAKDGPIDGAT
                     LDSFEANGFLSVDQLIAPEEVEDYRAELRRLSSDPQILADERTVTERGSDEVRSIFEVH
                     KISEVFAELVRDPRVVGRARQILGSEVYIHQSRVNYKPGFTGKDFYWHSDFETWHAEDG
                     MPRMRAVSISIALTENFVHNGGLMIMPGSHKTFVACVGETPDDHYKESLRGQEIGTPDP
                     NSLSILADKHGIELFTGPAGSATMFDCNCMHGSNGNITPFPRSNVFIVFNSVENTCVEP
                     FSAPAPRPSFIGARDFTPVGAGSVPEAAADRPPAEGTAGA"
     gene            complement(5001..5396)
                     /locus_tag="IEZ14_RS04365"
                     /old_locus_tag="GCM10010177_08860"
     CDS             complement(5001..5396)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657163.1"
                     /locus_tag="IEZ14_RS04365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08860"
                     /product="ectoine synthase"
                     /protein_id="WP_089312209.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-53, bitscore:
                     176.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLGEIIGTERDVQAPTWCSRRFVLAKEGVGFSLHETILYAG
                     TETKMWYANHIEAVYCVEGQGELTNDETGEKHKIEPGVMYLLDGHEHHTLRAHTDVRTV
                     CVFNPPCTGREVHDENGVYPLLTEEDA"
     gene            complement(5431..6693)
                     /gene="ectB"
                     /locus_tag="IEZ14_RS04370"
                     /old_locus_tag="GCM10010177_08870"
     CDS             complement(5431..6693)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 1.1e-72.
                     Score: 236.8. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS04370_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616167.1"
                     /locus_tag="IEZ14_RS04370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08870"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_179833978.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9e-74, bitscore:
                     244.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVFDRMESEVRGYCRSWPTVFTTAQGSHMTDENGKSYLDFFAGA
                     GTLNYGHNNPALKRRLLDYLAGDAVVHGLDMYTSAKREFLERFNEYVLAPRNLDYKVQF
                     PGPAGNNSVEAALKLARKYTGRETVVSFTNAFHGMTLGALAVTGNSMKRTGAGVPLGHG
                     VAMPYDNYLDGRTPDFLLFETMLDDSGSGLDKPAAVIVETVQGEGGINVATAEWLRGLQ
                     DLCHRQDILLIVDDVQMGCGRTGPFFSFEEAGITPDIVCLSKSLSGYGLPFAITLMKRE
                     LDVWEPGEHNGTFRGFNPAFVTAVGAMEDYWTGDDFEKQTLAKGQQVQAALNEIAITHA
                     GAIDSVRGRGLAWGLVMKDAEAAPRVCGEAFARGLLMETSGPEGEVVKLLPPLTTTESE
                     LATGLEIIADSVEAVRQKITA"
     aSDomain        complement(5620..6630)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS04370_Aminotran_3.1"
                     /evalue="1.10E-72"
                     /label="IEZ14_RS04370_Aminotran_3.1"
                     /locus_tag="IEZ14_RS04370"
                     /protein_end="358"
                     /protein_start="21"
                     /score="236.8"
                     /tool="antismash"
                     /translation="FTTAQGSHMTDENGKSYLDFFAGAGTLNYGHNNPALKRRLLDYLA
                     GDAVVHGLDMYTSAKREFLERFNEYVLAPRNLDYKVQFPGPAGNNSVEAALKLARKYTG
                     RETVVSFTNAFHGMTLGALAVTGNSMKRTGAGVPLGHGVAMPYDNYLDGRTPDFLLFET
                     MLDDSGSGLDKPAAVIVETVQGEGGINVATAEWLRGLQDLCHRQDILLIVDDVQMGCGR
                     TGPFFSFEEAGITPDIVCLSKSLSGYGLPFAITLMKRELDVWEPGEHNGTFRGFNPAFV
                     TAVGAMEDYWTGDDFEKQTLAKGQQVQAALNEIAITHAGAIDSVRGRGLAWGLVMK"
     gene            complement(6746..7294)
                     /gene="ectA"
                     /locus_tag="IEZ14_RS04375"
                     /old_locus_tag="GCM10010177_08880"
     CDS             complement(6746..7294)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657161.1"
                     /locus_tag="IEZ14_RS04375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08880"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_179833979.1"
                     /transl_table=11
                     /translation="MAAQPLESPTRRPGKNETNAEVQLQEPSLADGPALWRLARDSRVL
                     DVNSPYSYALWCRDFADTSVVARDGARPCGFITGYVRPSRPDTFFVWQVAVDRDHRGQG
                     LARRMLDSLADRLAPRGLEYMEATVTPDNTASTAMFESFAKARGCEISRSPLFAAEHFP
                     EGGHEPEVLFRIGPIAAAN"
     misc_feature    complement(7494..7512)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.71"
                     /tool="antismash"
     misc_feature    complement(7557..7572)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    complement(7692..7707)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.24"
                     /tool="antismash"
     gene            complement(7911..8879)
                     /gene="pip"
                     /locus_tag="IEZ14_RS04380"
                     /old_locus_tag="GCM10010177_08900"
     CDS             complement(7911..8879)
                     /EC_number="3.4.11.5"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (39-301). E-value:
                     1.5e-38. Score: 124.7. Matches aSDomain:
                     nrpspksdomains_IEZ14_RS04380_Abhydrolase_1.1"
                     /codon_start=1
                     /gene="pip"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657160.1"
                     /locus_tag="IEZ14_RS04380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_08900"
                     /product="prolyl aminopeptidase"
                     /protein_id="WP_179833980.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.7e-10, bitscore:
                     37.8, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 3.8e-34, bitscore:
                     114.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELRTLYPPIEPYDSGLLDVGDGNRIYWEVCGNPDGKPAVMLHGG
                     PGGGCSPAHRRQFDPEAYRIVLFDQRNCGRSLPHAKDPEVSLENNTTWNLVADMERLRE
                     HLGIERWLVFGGSWGSALSLAYAQTHPDRVTELVLRGIFTLRPFELYWFYQEGASLLFP
                     DLWEKYVEPIPEDEREDLISAFRERLDSPDLQVRIAAAKAWATWEGSTLTLRPDPEMAD
                     AFGEPEYAVAFARIENHYFSNEGFLEEDQLIRDVDRIRHIPAVIVQGRYDVCTPAATAW
                     DLHRAWPEADFHIVDDAGHAYSEPGILHRLIEATDRFAGKM"
     aSDomain        complement(7977..8762)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEZ14_RS04380_Abhydrolase_1.1"
                     /evalue="1.50E-38"
                     /label="IEZ14_RS04380_Abhydrolase_1.1"
                     /locus_tag="IEZ14_RS04380"
                     /protein_end="301"
                     /protein_start="39"
                     /score="124.7"
                     /tool="antismash"
                     /translation="VMLHGGPGGGCSPAHRRQFDPEAYRIVLFDQRNCGRSLPHAKDPE
                     VSLENNTTWNLVADMERLREHLGIERWLVFGGSWGSALSLAYAQTHPDRVTELVLRGIF
                     TLRPFELYWFYQEGASLLFPDLWEKYVEPIPEDEREDLISAFRERLDSPDLQVRIAAAK
                     AWATWEGSTLTLRPDPEMADAFGEPEYAVAFARIENHYFSNEGFLEEDQLIRDVDRIRH
                     IPAVIVQGRYDVCTPAATAWDLHRAWPEADFHIVDDAGHA"
     misc_feature    complement(9518..9532)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
ORIGIN
        1 cgagcgggtt gccggcggcc caccgccggg tcgccacggc gatcgcgccg agctgctcgc
       61 tcgtcgtgcc gtagcgggcc atgtgccgct gggcggcgag ggcgtagtgg gagttcacgc
      121 tgcgaaggcc gagcgccgcc gtcatcccgc cgaacccgta ccactccgcc ttcccggcgg
      181 gggcgtcgcg gttgtaggcc gatcccgccg actgcttcgg cttgagcggc gcgtccgcga
      241 acacgcacgc gaccgtggtc gccgaaccgt tcagcaccgc catcgccgcg taggacacca
      301 tcgccccggc cgtcgccccg taggagttga ccagggccag cagccgcagg tcgcgcaggc
      361 cgagcgtgtc ggccagctcg atcccgggcg aaccgctcat gccgtggctg accagcaggc
      421 cgtccaggtc gccgagcgcc aggcccgcgt cggcggcggc cagccgcacc gcgtcggccg
      481 cgagccgccg ggggctgcgc ccgtacaccc tgccgagctc ggtcatgccg agccccacga
      541 tcgaagtgcc catcgcctcg ctctcctcgt ccgtggggcc ccacggtaca gaatcgcgtt
      601 cggtacggga gcgggcgggg tgatcgggag ctggacgccg ccccgcgcgg gcggccccgg
      661 aacgggagtc gcccgcgtcc ccggggcgac ggggacgcgg gcgacgcgcg ggacacggtc
      721 ccgcccggcg gggccggacc ggtcaggagg cgaggacggc ctgcaggaag tcgcgggtgc
      781 gctgctcgga cggctcgccg aagatctggt ccggcggccc ctcctcgacg atctggccct
      841 ggtcgaacat caggacccgg tcggagatgt ccttggcgaa ggccatctcg tgggtgacgc
      901 agagcagggt caggtcgctc tgccgggcga tgtcgcgcag caggtcctgg acgccgacca
      961 cgagctcggg gtcgagcgcc gaggtgacct cgtccagcag cagcaccctc ggctgcatgg
     1021 ccagcgcccg cgcgatcgcc acccgctgct gctggccgcc cgacagctgg gtggggtgcg
     1081 cgttctcctt gtccgacagg ccgaccatgt ccagcaggcc gcgggcccgc tcgacggcct
     1141 cgtccttgga caccccgagc acgcgcaccg gcccctcggt gaggttgcgc agcacgttca
     1201 tgttcgggaa caggttgaac tgctggaaca ccatgccgac ctgcttgcgc atctcgtgca
     1261 ggtgcttctg gttcgccggg acgcgcttgc cgccgcggtc catgtgccac agcgtctcgt
     1321 cgccgatcca gatcagcccg tcgtcgagct tctccagcgt catcagcagc cgcaggatcg
     1381 tggtcttgcc ggacccgctc ggcccgatga gcgtcacccg ttcgccgggc gcgacggtga
     1441 agtccagctc gcggagcacg acgttggagc cgaaccgctt caccaccttc tcgaagcgga
     1501 tccccggcgc ctcgccgcgc ggcagccccg cggcgggatc ggccttcgtg gcggtgccct
     1561 tctgcgtgga gacgcccgcg tcggcggccg cctcgccggg ggcggggtcc tgcgccgcgt
     1621 tggcgccttc cgagggcggg cggtccgcct cgcccccggg gtcgactgcg tcggtgtcaa
     1681 ttggtggcat aacgcctctc caagcggcgg acgagtatcg aggatatgac gctgaccacg
     1741 acgaacaaca ggccgaccat ggtgatcggc tccaggaact gcagggtctt ggacccgacc
     1801 tggtacgcgg agaacagcag ctccgagacg ttgatcgcca gcagcagcgg cgaatccttg
     1861 aacatcgcga tgaggtagtt gccgagcgtc gggatcaccc gccggatcgc ctgcggcagg
     1921 acgacgtcca gccacacccg cgaacgcggc aggttcagcg cggtgcacgc ctcccactgc
     1981 cccttgggga cgtcggcgat cccggcccgg tacacctcgg ccgtgtaggt cgagtagtgg
     2041 atcccgagcg tgatggtgcc cgcggtgagg gacccgatgg tgatgcccca ggcgggcagc
     2101 acgaagaaca cgaagtacag ctgcgggatc agcggcgtgg acctgatgaa ctcggtgacc
     2161 caccggatca cctggttgaa gacccggctc ggggagcggc gcagcagcgt ccacaccagc
     2221 ccgaggacca gggcgatcag atagcccagc agcgtcgcgg tcagcgtgta gcgcagcccg
     2281 tccagcaggt cggggaggat ctcgccggag tagtgccagt cccagctcat cagcccaccc
     2341 ccgccccggg cgcgccggcc gcgatcgtcc cgagggacgg ctcagcgggc agcctgcggc
     2401 ccagcatccg gtccacgcgc cgctcggcgt agcggacgaa cagctgcaga acctgcgcga
     2461 tcacgaagta caggacgagc acgaccgtga acgcggcgac ggtctggccg gtgctgccct
     2521 ggatgttctt ggagacgcgg gtcaggtcga ccaggcccac cagggacacg atggcggtgc
     2581 ccttcatcag ctcgatgatc aggttgccga acgggggcag catcagcgcg aacgcctgcg
     2641 gcagcaggac ccggcgcatg cgctggaacg gagtgaagtt cagcgcgatc gtcgcctcgt
     2701 actgcgcctt cgggacggcg ttgatcgatc cgcgcaccac ctcggcgccg taggcgccga
     2761 cgttcaggcc gagggccagc acggccgcga acatcgtgga gaaccggaga ccgatcaggg
     2821 gaagggcgta gaagaaccag tacagcagca ccagcgtggc gttgccgcgg aagaactcca
     2881 cgtacacgcg gttgagcacg cgcacccacg cgtggcgcga cgtgcccgcc atcccgaaga
     2941 cgagcgcgag caccaacgcc agcgcgccgc cgaggagggt gagccggatg gtgacccacg
     3001 ccccgtccag ccactgggga tagctgtcga ggacctcgga cacgggtcag ccctggcaga
     3061 acttggcggc ggtgtcgtcc gggccgggca tctccgcctg ggtgaagccg aacggctgca
     3121 ggatcggcag cagcttgttg ccctgctgga gcttggcgag ctcgccgttc caggcgttca
     3181 ccaggtccgt gtcggccttg cggaaggcga aggcgccggc gccgagctgc tccttgccgt
     3241 cgatgacggg ggtgaacgcg tcggtgacct cgaagccctc gcccttgtgc ttggtcagca
     3301 ggtcggccag cgagatgcgg gtcagccaga tgcagtcgat gcgcccggcc ttcaggccct
     3361 cgtaggcgct cgcctggtcg gcgaacgtct tgatgtcgcc cttcttgacg ccggtcttct
     3421 cggcgtaccc ggcctcgacg gcgccggtga gcacgccgac cttgacgccc ttcttgcccg
     3481 ggtcctcggc ggccttgagg cccttcgggt tgccctcggg caccatgaag gcgctcttgg
     3541 cgacgtactc ggggttggcg aacgccgcgg aggcgcaccg ctcgggcgtg atgaacatgc
     3601 cggcggcgat cgcgtcgaag cgcttggcgt tgagcccgcc gatgagcccc ccgaagtcga
     3661 cctggacgcc cttgatctcg tcgatgccga ggttcttgaa gatggcgcgc gcgagctcag
     3721 gggcctcacc ggtcagcttc ccggacttgt cggtgtagcc gtagggcgcc tcgttggcga
     3781 acccgaccgt gatcgtgccg gagtccttgg ccttctgcag cgtgccgccg ccggtcttct
     3841 ccttctccgg atccgtcgtg gagcagcccg ccgcggccag cggcgccgcg gcggacagca
     3901 gcacggcggt gcggaggaag tcgcgtctcg aagaagtcat cgttgaactc ctgatgggtg
     3961 agctccgcgc ccgcagggag gccggacggc ccccgtgcgg gcgcggagcg ggtgggtcgg
     4021 gttatcgaat tgttaggctc aggcgccggc ggtgccctcc gcggggggac ggtctgccgc
     4081 ggcctccggg acgctcccgg ctccgacggg ggtgaagtca cgggcgccga tgaaggaggg
     4141 acggggcgcg ggtgcggaga acggctcgac gcacgtgttc tccacgctgt tgaacacgat
     4201 gaacacgttg gagcgcggga acggggtgat gttcccgttc gagccgtgca tgcagttgca
     4261 gtcgaacatg gtcgccgagc ccgcggggcc ggtgaacagc tcgatcccgt gcttgtcggc
     4321 caggatcgac aggctgttgg ggtcgggtgt accgatctcc tggccgcgca gggactcctt
     4381 gtagtggtcg tccggggtct ccccgacaca ggccacgaag gtcttgtgcg agccaggcat
     4441 gatcattagc ccgccattgt gcacgaaatt ctcggtgagc gcgatggata tgctcaccgc
     4501 gcgcatccgg ggcatgccgt cctccgcgtg ccaggtctcg aagtccgagt gccagtagaa
     4561 gtccttgcca gtgaagcctg gcttgtagtt gacccggctc tggtggatgt aaacctccga
     4621 gccgaggatc tgcctggccc ggcccactac gcggggatcg cggacgagct cggcgaacac
     4681 ctcgctgatc ttgtgcacct cgaagatgga gcggacctcg tccgagccgc gctcggtcac
     4741 cgtgcgctcg tcggccagga tctgcggatc cgacgacagc cggcgcagct cggcccggta
     4801 gtcctcgacc tcctccggcg cgatcagctg gtcgacggac aggaacccgt tggcctcgaa
     4861 ggagtcgagg gtcgcgccgt cgatgggccc gtccttcgcc tcgccgtaaa cgaccgggtc
     4921 ctggcggtac agcaatgcgg cctcggaggc cttccgggtg gggtaggcgt cgtcgatcgt
     4981 cggatgggac tcgatgatgc tcatgcgtcc tcctcggtca gcagggggta gacaccgttc
     5041 tcgtcatgga cctcccggcc ggtgcagggc gggttgaaca cgcagacggt ccgcacgtcg
     5101 gtgtgggcgc gcagagtgtg gtgctcgtgg ccgtcgagca ggtacatcac gcctggctcg
     5161 atcttgtgct tctcgcccgt ctcgtcgttg gtcagctcgc cctggccctc gacgcagtac
     5221 acggcctcga tgtggttggc gtaccacatc ttcgtctcgg tccccgcgta caggatcgtc
     5281 tcgtgcagcg agaacccgac gccctctttg gccagcacga agcggcggct gcaccaggtc
     5341 ggcgcctgca cgtccctttc cgtgccgatg atctctccga gggaacgaac gatcattgaa
     5401 actcctttgt tctttcggtg ccggaccggc tcaggccgtg atcttctggc ggacggcctc
     5461 caccgagtcc gcgatgatct ccaggccggt ggccagctcg gactcggtcg tggtgagcgg
     5521 cggcagcagc ttcacgacct cgccttcggg gccggaggtc tccatcagca gcccgcgggc
     5581 gaacgcctcg ccgcacaccc tcggcgccgc ctccgcgtcc ttcatcacca ggccccacgc
     5641 caggccccgg ccgcggacgg agtcgatggc gccggcgtgc gtgatcgcga tctcgttcag
     5701 ggccgcctgg acctgctggc ccttggcgag ggtctgcttc tcgaagtcgt cgccggtcca
     5761 gtagtcctcc atcgcgccga cggcggtgac gaacgcgggg ttgaagccgc ggaaggtgcc
     5821 gttgtgctcg cccggctccc acacgtccag ctcgcgcttc atcagcgtga tcgcgaaggg
     5881 cagcccgtag ccgctcaggg acttggacag gcagacgatg tccggggtga tcccggcctc
     5941 ctcgaagctg aagaacggcc cggtgcggcc gcagcccatc tgcacgtcgt cgacgatgag
     6001 caggatgtcc tgccggtggc acaggtcctg caggccgcgg agccactcgg cggtggccac
     6061 gttgatgccg ccctcgccct gcacggtctc cacgatgacg gcggcgggct tgtccaggcc
     6121 gctgccgctg tcgtccagca tcgtctcgaa gagcaggaag tcgggcgtcc tgccgtcgag
     6181 gtagttgtcg tagggcatcg ccacgccgtg cccgagcggc acgcccgcgc cggtgcgctt
     6241 catcgaattg ccggtgacgg cgagcgcgcc gagcgtcatc ccgtggaagg cgttggtgaa
     6301 gctgacgacg gtctcgcggc cggtgtactt gcgcgccagt ttcagcgccg cctcgaccga
     6361 gttgttcccc gcgggcccgg ggaactggac cttgtagtcg aggttgcggg gcgccaggac
     6421 gtactcgttg aagcgttcca ggaactcccg cttggccgag gtgtacatgt cgagaccgtg
     6481 cacgacggcg tcgccggcga gatagtccag cagccggcgt ttcagcgccg ggttgttgtg
     6541 cccgtagttg agggttcctg ctccggcgaa gaagtcgagg tagcttttgc cgttctcgtc
     6601 ggtcatgtgg ctgccctgtg ccgtggtgaa caccgtgggc cagcttctgc agtagccgcg
     6661 gacttccgat tccatgcggt cgaaaacgtc catgtctgcc tccaggcagg gtcggagagg
     6721 agcggatggg ggcgtggagc gtggatcagt tcgcggcggc gatcgggccg atcctgaaga
     6781 gcacctcggg ctcgtgtccc ccctcgggga agtgctccgc ggcgaacagc gggctgcgag
     6841 agatctcgca cccgcgggcc ttggcgaagg attcgaacat cgcggtggag gcggtgttgt
     6901 ccggcgtgac cgtggcctcc atgtactcga gcccgcgggg ggcgaggcgg tccgccaggc
     6961 tgtcgagcat gcggcgggcg aggccctggc cacggtggtc gcggtcgacc gcgacctgcc
     7021 atacgaagaa cgtgtccggg cgggaagggc ggacgtagcc ggtgatgaag ccgcacgggc
     7081 gggcgccgtc gcgtgcgacg acggaagtgt ccgcgaaatc gcggcaccag agcgcgtagc
     7141 tgtacggcga gttgacgtcc aggactcttg agtcccgggc gagccgccaa agcgcgggtc
     7201 catcggcgag gctcggctcc tggagttgca cttccgcgtt cgtctcgttc ttcccggggc
     7261 gtcgggtcgg tgattccagg ggttgcgctg ccatgtccag gcaatttacc gaatctcgcg
     7321 cgatgatcgc agatagatgt tatcgacgag atgtttagcc cgatcatcag tgggtatgat
     7381 tgagggcttt cccgattcgg gtgcaatttg ggcggtcttc aggcgggccg cgatccgctt
     7441 ttccccggga tacgcaggag gtactgccgg gtgcgccgga gatgttgccg gcatgcttcc
     7501 tgaatgcaac cttaacgttt cgccgtggtg ccaggagtgc ttttctgtat cggttatgtg
     7561 aggtaagtca catatatccg gtgtcaacgg ggcgggtgcg gcgggcgtca cttcgcatcg
     7621 cgggaggtcg ggccgcgacg gggcgaggtg gggcgggtgt gggccgaagc actccgcgag
     7681 gccgtctgcg ctacccctca ggggcaggtg cgctatccag agggtaaggc cagggttgct
     7741 aacaggacag ggaatgtggg ttttggtccg cttccggggg gccggggccg ggcaaggaaa
     7801 ccggaccccg gcccggttgc gggcatgtcg aagctcgtta ttggtgaggc cgattcagat
     7861 ccggccttcc gccgtccgat gacgtaatgt tatcaggcga tgacgaaggg tcacatcttc
     7921 ccggcgaacc ggtcggtcgc ctcgatcagg cggtgcagga tccccggctc gctgtaggcg
     7981 tgccccgcgt cgtcgacgat gtggaagtcc gcctcgggcc acgcgcggtg caggtcccac
     8041 gccgtcgcgg ccggcgtgca cacgtcgtag cggccctgca cgatcacggc ggggatgtgg
     8101 cggatcctgt ccacgtcccg gatcagctgg tcctcctcga ggaacccctc gttgctgaag
     8161 tagtggttct cgatgcgggc gaacgccacc gcgtactccg gctcgccgaa ggcgtcggcc
     8221 atctccgggt ccggccgcag cgtgagcgtc gagccctccc acgtcgccca cgccttcgcg
     8281 gccgcgatcc gcacctgcag gtccggcgag tcgagccgct cgcggaacgc ggagatgagg
     8341 tcctcgcgct cgtcctcggg gatcggctcg acgtacttct cccagagatc ggggaacagc
     8401 agcgacgcgc cctcctggta gaaccagtac agctcgaacg gccgcagggt gaagatgccg
     8461 cgcagcacca gctcggtcac ccggtcgggg tgcgtctgcg cgtacgccag cgacagggcg
     8521 ctgccccagc tcccgccgaa caccagccag cgctcgatgc cgaggtgctc gcggagccgt
     8581 tccatgtcgg cgaccaggtt ccaggtggtg ttgttctcca gcgacacctc cgggtccttc
     8641 gcgtgcggca ggctgcgacc gcagttgcgc tggtcgaaca ggacgatccg gtacgcctcg
     8701 gggtcgaact gccggcggtg ggccgggctg cacccgccgc ccggcccgcc gtgcagcatc
     8761 accgcgggct tgccgtcagg gttcccgcag acctcccagt agatacggtt tccgtcgccg
     8821 acgtcgagca gcccggagtc gtagggctcg atcggcgggt acagcgtgcg aagctccatg
     8881 gggcgcgatt gttccacccg gcccgggccg cggccggatg tgacccacgg cacctcgcgg
     8941 acgggccgcg cggacggcga tcaggcgtcc ggcgtgcgcg aacgccgggc gggtgtcgag
     9001 aaggcaaacc ccaaactggg caaacgttcc gctggtcggg actagagtgc tcctagtgag
     9061 cgaagcgagg agccacaggg ccggcagcgg agcgagcgag atgtgcacaa cgacccccgc
     9121 gaacgcggcc gcgcgccgcc gggcgaggag acacgtgagc gaagtgacga gccgtcgggc
     9181 cgggaacgct gcgagcaggg cgagcggcat gccgccggtc ggcccgctcc cgtccgggag
     9241 gcgaggagac cggtgagcga gctgaacgga tccggcggtt cccgaggagt ccccgacgcg
     9301 gcgactccct ggcccgccat gggacgcgac ccgggcggcg cgcagcaggc gctgccgtcc
     9361 gcccagcagc ccatccagat gtccaagcca gggcagcccg cgcaggccgg ccagcccgcg
     9421 cagccgggcc ggcacgggca gacgggggag atcgtgcgcc ccgacgtcga gcagggcgag
     9481 gcccagccct gggacatcgt cgagcggctc cgccagatgg ccgacctcat cggtgacgcg
     9541 ctcgccgccg gggagcgcaa ggtcaacgag gcgcagaccg agacggcgac gcagatcgcg
     9601 gccgtgcagg cggagaagga gtcggcgcag cgcgacgcgg ccgccgcctg gggcgaggtg
     9661 cagcgcgccc gcggccaggc cgagcaggcc gaccgccgcg ccgtggaggc cgagcgccgc
     9721 gtcaccgagg cccagaacga gaccgcggca cagatcgcgg ccgtgcaggc ggagaaggag
     9781 tcggcgcagc gcgacgcggc cgccgcctgg ggcgaggtgc agcgcgcccg cggccaggcc
     9841 gagcaggccg accgccgcgc cgtggaggcc gagcgccagg tcgccgacgc cgagcgccgc
     9901 gccgccgagg tgcagaacga ggccgtcacc cagatcgcgg ccctccagac cgagaaggag
     9961 aacgcccagc gcgacgcggc cgccgcctgg aaccaggccc agcagggccg cgtccaggcc
    10021 gaccaggccc gcggccaggc cgagcaggcg cgcgtccagg ccgaccaggc gcgcgcacag
    10081 gtcgagcagg cccgcgcgca gatggagcag ttgcaggccc aggccgagca ggccgagcgg
    10141 cgcgtcgccg aggccgaggc gaccgtccgg caggcgatcc agcagcgcga cgccatgagc
    10201 gaggcccgcg acgacgccct gcgcgccgtc gaggacgccg tcggcggccg ccgctccgcc
    10261 gagaccgagc gcgaccgcgt cgccgagcag gccggggagc tgtcgcgcgc gctggagacc
    10321 gcgcacgccg agctgtcggc gctgcgcgcc aaggtcaccg acgccgagat gagcgcgcag
    10381 aacctgcagc acgccg
//
