LOCUS       NZ_BMRD01000001        10810 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Actinomadura citrea strain JCM 3295 sequence01, whole genome shotgun
            sequence.
ACCESSION   NZ_BMRD01000001
VERSION     NZ_BMRD01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Actinomadura citrea
  ORGANISM  Actinomadura citrea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 994668)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 127X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648455.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:26:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,339
            CDSs (total)                      :: 8,265
            Genes (coding)                    :: 8,175
            CDSs (with protein)               :: 8,175
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 12 of 90
            Pseudo Genes (incomplete)         :: 83 of 90
            Pseudo Genes (internal stop)      :: 3 of 90
            Pseudo Genes (multiple problems)  :: 8 of 90
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:33:41
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 641465
            Orig. end                         :: 652275
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMRD01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10810
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10810
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10810
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5810](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5810
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(97..972)
                     /locus_tag="IEZ14_RS03050"
                     /old_locus_tag="GCM10010177_06180"
     CDS             complement(97..972)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630858.1"
                     /locus_tag="IEZ14_RS03050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06180"
                     /product="hypothetical protein"
                     /protein_id="WP_179833761.1"
                     /transl_table=11
                     /translation="MNRRGNGLSADTYAPLVDLAPHLADAMLSALGEAGVAAYAASPSG
                     LPEIAEAAAEPVAGDVLDRLYVDVEMKPAAEGILRVHLARLREASARGSDGEREGAELA
                     DADPLGTDPLGTDRPDTAERAGTGLADPGKGKAGGDAERDEDAIWAEIVAGYDAVPEGD
                     EVPWPDEENIREPADPPGEGEDRTGRLPRARVIKPVEETAFPAGDDDDDEGHYVPPPPP
                     PLPSADPLTKGAWIALIGGPVYLLVTVILDWEVPGWAAFLAVAAFIGGFVTLVLRMGDE
                     PRDPDDGAVV"
     gene            1341..2075
                     /locus_tag="IEZ14_RS03055"
                     /old_locus_tag="GCM10010177_06200"
     CDS             1341..2075
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654832.1"
                     /locus_tag="IEZ14_RS03055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06200"
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="WP_179833762.1"
                     /transl_table=11
                     /translation="MLWFWILLRIVLSPVMYLLWWPRNRGAGNVPKRGPALMVSNHLSF
                     ADHFFGPLPLMRPIYFIGKGEYFTGRGIKGLVSKAFFTGVGVVPVDRTGGSAAEAALQT
                     GLRILGEGKLLGIYPEGTRAPDNRLYRGKTGVARLALKSRVPVIPMAMVNTFELMPPGR
                     PYPRLGIRPGVRFGEPLDFSRHYGREDDHEVLREITDEIMMAIGELSGQEYVDRYAAEV
                     KAEMAGREVRPVEDDGGLSDGG"
     gene            2062..3246
                     /gene="macS"
                     /locus_tag="IEZ14_RS03060"
                     /old_locus_tag="GCM10010177_06210"
     CDS             2062..3246
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="macS"
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017557771.1"
                     /locus_tag="IEZ14_RS03060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06210"
                     /product="MacS family sensor histidine kinase"
                     /protein_id="WP_229809899.1"
                     /transl_table=11
                     /translation="MTAAEPRGTGLETALWRAVAVYRGLALCYAAVVIAMNSGGYAHPL
                     GGWAVLAVMAGWTGYASFAFGPAAFASGGPGRRGRALAAADLAVAAGCVLATAWVETPA
                     NIAAGRPTLPVSWVAAAVLAWAVVGGRRAGIAAAAVLAAANLLVHALAGESGGIGGTTF
                     NGIVLLFLAGLVVGHVVRLAREAEARLARAVELEAATRERERLARRIHDSVLQVLAMVQ
                     RRGGELGGEAAELGRLAGEQEAALRSLVGAGPAAPPAAAPGAGTDLRALLTGHASTSVT
                     VSTPATEVRLPAHAAGEVEAAVAAALDNVRRHCGDEARAWVLLEDGEGGVTVSVRDDGP
                     GMPPGRLDRAAADGRLGVAQSIRGRIADLGGTVSIVSAEGDGTEVEMTVPRARV"
     gene            3282..3920
                     /locus_tag="IEZ14_RS03065"
                     /old_locus_tag="GCM10010177_06220"
     CDS             3282..3920
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630855.1"
                     /locus_tag="IEZ14_RS03065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06220"
                     /product="response regulator transcription factor"
                     /protein_id="WP_179838538.1"
                     /transl_table=11
                     /translation="MVVDDHPMWREAVARDLAEAGHDVVATAGEGHAAVRIAAAARPQV
                     AVVDLQLPDLSGVEVTRRLAAADPPVRVLVLSASGEQQDVLEAVKAGATGYLLKSAARE
                     EFLDAVRRTGEGDAVFTPGLAGLVLGEYRRLAAAPAREDDDAPRLTERETEVLRLVAKG
                     LTYKQIAQRLVLSHRTVQNHVQNTLGKLQLHNRVELVRYAIEKGLDEEQ"
     gene            3989..4999
                     /locus_tag="IEZ14_RS03070"
                     /old_locus_tag="GCM10010177_06230"
     CDS             3989..4999
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="IEZ14_RS03070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06230"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_179833763.1"
                     /transl_table=11
                     /translation="MADEILGPALGFLAAHVLVGLVLLVILRRMRAAVLRGRPPARELH
                     PYEVAYLNGGGRHAIAASVTALRLDGAADAYADGRLLAREPSADAPRAAGTPLDTAVHW
                     AIERLRAETLAQITADPDVRAALRRLRDGLAAQGMVLGQAGRRRLRNLQLVMYGWILLG
                     FAAFVLSDAFDGFPAKLIPLAALVGLGFGALALGQGAERTREGERAVERLKESHSALDP
                     ANGPSYTGLPAPAALMGVALFGAAALMALDPVFVQTVGLGRYLEMAGVVAASGGSSGAS
                     GSSTSFVCSTTAAVCSSSSCGGGGSCGGGSGCGGGSGCGGGGGGGGGGCGGGGGS"
     misc_feature    complement(3990..4004)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.71"
                     /tool="antismash"
     gene            5001..5810
                     /locus_tag="IEZ14_RS03075"
                     /old_locus_tag="GCM10010177_06240"
     CDS             5001..5810
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="IEZ14_RS03075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06240"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_179833764.1"
                     /sec_met_domain="DUF692 (E-value: 4e-78, bitscore: 258.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGVGIGWRPEIAGFVGALPGLRFTEVIAESVHPAGPATELLAL
                     RDRGAAIVPHGVRLSLGGAEPVDAERVAHLAACAEALGAPLASEHVAFVRAGGVEAGHL
                     LPVPRTRAALDALTANIRRTQAELPVPLAVEPIAALFEWPDAEYAEADFLTELLERTGA
                     LLLLDVANVYANARNRGEDPADLLDRLPLDRIAYCHVAGGSQEGGRYHDTHAAAVPAEV
                     LDLVAELCERHRPPGLLLERDGRYPPAAELRAELDAIAAASGLAPVT"
     gene            5824..6270
                     /locus_tag="IEZ14_RS03080"
                     /old_locus_tag="GCM10010177_06250"
     CDS             5824..6270
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEZ14_RS03080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010177_06250"
                     /product="hypothetical protein"
                     /protein_id="WP_179833765.1"
                     /transl_table=11
                     /translation="MSEFDAGLAAAQEALVRAMTAGGPLPEGFDAEAVGAAAHGILLKR
                     AGEVARAWPALAASYGTSWKAAFAAWAAERPTRGSFRDGWDFARDRGRLAGDAARELAL
                     AEVRWSYDGQDPPRPRGAAARRVPGGAAVVFRGRVRVFGRGRSG"
     misc_feature    complement(6317..6332)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 25.87"
                     /tool="antismash"
     gene            6439..7464
                     /locus_tag="IEZ14_RS03085"
                     /old_locus_tag="GCM10010177_06260"
     CDS             6439..7464
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017565081.1"
                     /locus_tag="IEZ14_RS03085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06260"
                     /product="6-phosphofructokinase"
                     /protein_id="WP_179833766.1"
                     /transl_table=11
                     /translation="MRVGVLTGGGDCPGLNAVIRAVVRKGVQVYGYEFVGFRAGWRGPL
                     EGDTVALDVQAVRGILPRGGTILGSSRTNPVKVEGGVERIKDNLAGLGVDALIAIGGED
                     TLGVASELYANGVNVVGVPKTIDNDLNATDYTFGFDTAVNIGMEAVDRLHTTAESHHRA
                     LICEVMGRHAGWIALHVGMAAGANVILIPERPFDIEKVCQYVESRFKTRYAPIIVVSEG
                     AHPVDGQMQLQTGERDAFGHVRLGGIGQALADEIEKRTGKEARATVLGHIQRGGTPTAF
                     DRVLATRFGLQAIDAVKDGDYGKMVALQGTDIIRVGLDEATKELKTVPLERYSEAEVFF
                     G"
     gene            7565..8821
                     /gene="thiI"
                     /locus_tag="IEZ14_RS03090"
                     /old_locus_tag="GCM10010177_06270"
     CDS             7565..8821
                     /EC_number="2.8.1.4"
                     /GO_function="GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thiI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654826.1"
                     /locus_tag="IEZ14_RS03090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06270"
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="WP_179833767.1"
                     /transl_table=11
                     /translation="MTTLDTAPDAAPGTAAADVREQSPVNGEPCVLLKLGEVVLKGKNR
                     EQFERRLADNVRTAVRPIARVDVVRRHGVFIVRRPGADLATMERVAQRITDVMGIVWAH
                     RAWQVGKDLASVERAALELMDGRTGTFAVRSRRRDKRFPMTSTELDRHIGTLVTARYGQ
                     PVRLKNPEHTLSIEVDRDEVFVYSGGLPGQGGLPVGMSGRGLVLMSGGIDSPVAAYRMM
                     RRGLRVDYLHFSGMPFTGPESIYKAYALVRELDKFQTGSRLFVVPFGKAQQQIKSSGAD
                     RLAVIAQRRLMLRTGEVLARRLRGSALITGDALGQVSSQTLANITALDDAVELPILRPL
                     VGMDKVEIMDQARRIRTLTISELPDEDCCTLLAPRRAETRAKIGDLRQIEKRLDVGELA
                     DQLAASVQEHRPAYGDPAT"
     gene            complement(8846..9748)
                     /locus_tag="IEZ14_RS03095"
                     /old_locus_tag="GCM10010177_06280"
     CDS             complement(8846..9748)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039603.4"
                     /locus_tag="IEZ14_RS03095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06280"
                     /product="DUF5954 family protein"
                     /protein_id="WP_179833768.1"
                     /transl_table=11
                     /translation="MTTGDGDAGGRLFPEDLDGVDPVAAVLLADARRALAAYPELVALG
                     AVYTAAERVPGGWQVVCPFDPLPQGAREILADHLEDRAAPGSGARARDLAAAARTLRRE
                     PADEVTAGGRRFRIVRIEQLARTGPDGPEPPRPTDLDPLPACGRVPPARPHELLPDDGP
                     ASDLATAELLCQVLDAAAATGNEPSGAFLTPVALAPAFTVAERGERRWRPVGRLYDAPQ
                     QARDSLATYFRHVVPAVESPAATDVARFAEAAELMEDDSRRNGIAVAGRRFRIVRIERI
                     TLMGPDGPEPPRPTDFDAR"
     misc_feature    complement(9783..9798)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.63"
                     /tool="antismash"
     misc_feature    9784..9798
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.24"
                     /tool="antismash"
     gene            complement(9789..10775)
                     /locus_tag="IEZ14_RS03100"
                     /old_locus_tag="GCM10010177_06290"
     CDS             complement(9789..10775)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630851.1"
                     /locus_tag="IEZ14_RS03100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010177_06290"
                     /product="DMT family transporter"
                     /protein_id="WP_179833769.1"
                     /transl_table=11
                     /translation="MSQNTSLGRAPEKSSRSRARRGGADVLLAATLWGTTGTVRTFADG
                     ASPYSVAAIRIIIGGLVLLALAALTRGGAGLRHLVADRRNLPLVGLGAVAITVYQTAFF
                     VAAGRTGVAVGTIVTIGSAPVFTGVIGLLARRSAPTGRWTLATAGAVAGCALLVGGGRD
                     AGAEPLGIGLALLSGLAYAAYATVASVLITRGEQDRAVAAALFGTAALPLVPVLLAGPT
                     GWLVTGSGALIALYLGVITTGGGYLLFARGLRTTPATTATTLTLAEPAVAAVLGTVLLG
                     ERLGGAALGGLVLLATSLVVLVAPRRRGRGEGAQGRGEGAQGGNEMV"
     misc_feature    10763..10772
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.04"
                     /tool="antismash"
ORIGIN
        1 cgtgcggatc agcgcgtgcg gggtgccgcc ggacgacgcc ggcgaccatc ccctggccac
       61 cgaccaccgg cccctgctgg ccgagctggc gctgccctag acgacggccc cgtcgtcggg
      121 gtcgcggggc tcgtcgccca tgcgcaggac gagggtgacg aagccgccga tgaacgcggc
      181 gacggccagg aacgcggccc agccggggac ctcccagtcg aggatgacgg tgaccagcag
      241 gtagacgggg ccgccgatga gcgcgatcca ggcgcccttg gtgagggggt cggcgctcgg
      301 cagcggcggg ggagggggcg ggacgtagtg cccctcgtcg tcatcgtcgt caccggccgg
      361 gaacgccgtt tcctcgaccg gcttgatcac gcgggcgcgg ggcagccgcc cggtgcggtc
      421 ctcgccctcg cccggggggt cggcgggctc gcggatgttc tcctcgtcgg gccacgggac
      481 ctcgtcgccc tcggggacgg cgtcgtaccc ggcgacgatc tcggcccaga tggcgtcctc
      541 gtcgcgctcg gcgtcgccgc ccgccttgcc cttgcccggg tcggcgaggc cggtcccggc
      601 gcgctcggcg gtgtccggcc ggtcggtgcc gagcgggtcg gtgccgagcg ggtcggcgtc
      661 ggcgagctca gcgccctcgc gctcgccgtc ggagccgcgg gcggaggcct cgcgcagccg
      721 ggcgaggtgc acgcgcagga tgccctcggc ggccggcttc atctcgacgt ccacgtacag
      781 ccggtcgagg acgtcgccgg cgaccggctc agcggccgcc tcggcgatct ccggcaggcc
      841 ggacggggac gccgcgtagg ccgcgacgcc cgcctcgccg agcgccgaca gcatcgcgtc
      901 cgcgagatgc ggcgcgaggt cgaccagtgg ggcgtaggtg tccgctgaca gtccattgcc
      961 acggcgattc acggggccgg ctccttcgac gactccggcg tgaccgacag caccggcatg
     1021 gcggcctccg ctccccccat tgacatgtgg tgcccggtcg cccccgtgcg gaggcactcc
     1081 cggacacccc gaaacgtgtg tcccacggta ttcgctgcgc cgcccgggac aagcccccgg
     1141 gcgcacccga ttgagattta ccgctccgag cctacggtta ttcccggacc gccgccgggg
     1201 cgcggccggg ttccggtgac gggtaccggc cggtagatcg ctgggacgga gagtgggagg
     1261 atgtcccatc atcccgtcgg ccaggaccgg ggcacgcccg cgcgggggcg tccggtccga
     1321 gggaaaggta cccgcatacg atgctctggt tctggatcct gctgaggatc gtcctctccc
     1381 cggtcatgta cctgctgtgg tggccgcgga accggggggc gggcaacgtc ccgaagcgcg
     1441 ggccggcgct gatggtgagc aaccacctgt cgttcgccga ccacttcttc ggcccgctgc
     1501 ccctgatgcg cccgatctac ttcatcggca agggggagta cttcaccggc agggggatca
     1561 agggcctggt gagcaaggcc ttcttcaccg gcgtcggggt ggtcccggtc gaccggaccg
     1621 gcggctcggc ggccgaggcg gcgctgcaga ccgggctgcg gatcctcggc gagggcaagc
     1681 tgctcggcat ctaccccgag ggcacccggg cccccgacaa ccggctgtac cgcggcaaga
     1741 cgggggtcgc gcggctggcg ctgaagtcgc gggtgccggt gatcccgatg gcgatggtca
     1801 acacgttcga gctgatgccg cccggcaggc cgtacccgcg gctcgggatc cggccgggcg
     1861 tccggttcgg cgagcccctg gacttctccc gccactacgg ccgcgaggac gaccacgagg
     1921 tgctccgcga gatcaccgac gagatcatga tggcgatcgg ggagctgtcg gggcaggagt
     1981 acgtggaccg gtacgccgcc gaggtgaagg ccgagatggc cggcagggaa gtgcgtccgg
     2041 tcgaggacga cggagggctg agtgacggcg gctgagccgc gcgggacggg cctggagaca
     2101 gcgctgtggc gggccgtggc cgtctaccgc gggctcgccc tgtgctacgc ggcggtcgtg
     2161 atcgccatga actccggcgg ctacgcccac ccgctcggcg gatgggccgt cctcgccgtc
     2221 atggccggat ggaccggcta cgcgtcgttc gccttcgggc ccgccgcgtt cgcctccggc
     2281 ggcccgggac gccgcggccg ggcactggcc gcggccgacc tcgccgtcgc ggcggggtgc
     2341 gtgctggcga cggcgtgggt ggagacgccc gcgaacatcg ccgccgggcg cccgacgctg
     2401 ccggtgtcct gggtggcggc cgcggtgctg gcgtgggcgg tcgtgggcgg gcggcgggcc
     2461 ggcatcgccg ccgcggccgt cctggccgcc gcgaacctgc tggtccacgc gctcgccggg
     2521 gagagcggcg gcatcggcgg cacgacgttc aacggcatcg tgctgctgtt cctcgccggc
     2581 ctcgtcgtcg ggcacgtcgt gcggctggcg cgggaggcgg aggcgcggct ggcgcgggcc
     2641 gtcgagctgg aggcggcgac ccgtgagcgg gagcggctgg cgcgccgcat ccacgactcg
     2701 gtgctgcagg tcctggcgat ggtgcagcgg cgcggcggcg agctcggcgg cgaggcggcc
     2761 gagctcggcc gcctcgcggg cgagcaggag gcggcgctgc gctccctcgt cggcgcgggc
     2821 ccggcggcgc cgcccgcggc cgcccccggc gccgggacgg acctgcgtgc gctgctgacc
     2881 gggcatgcct ccacctccgt gacggtctcg acgcccgcca cagaggtgcg gctgccggcg
     2941 cacgccgccg gggaggtcga ggcggcggtg gcggcggccc tggacaacgt ccggcggcac
     3001 tgcggcgacg aggcccgcgc gtgggtcctg ctggaggacg gtgagggcgg ggtcaccgtc
     3061 agcgtccgcg acgacggccc gggcatgccc cccgggcggc tggaccgggc ggcggcggac
     3121 gggcggctcg gcgtcgccca gtccatccgc ggccgcatcg ccgacctcgg cggcaccgtg
     3181 tcgatcgtct ccgcggaggg cgacgggacc gaggtcgaga tgaccgtgcc gcgggctagg
     3241 gtgtgaccgt gagcgacgtt cccctccgac ccctccgggt catggtcgtg gacgaccatc
     3301 cgatgtggcg cgaggcggtg gcccgcgacc tggccgaggc cggccacgac gtggtcgcga
     3361 cggcggggga gggccacgcc gccgtccgca tcgccgccgc cgcgcgccct caggtagccg
     3421 tggtcgacct ccagctgccc gacctcagcg gggtggaggt gacccggcgc ctcgccgcgg
     3481 ccgacccgcc cgtccgcgtg ctggtgctgt cggcgagcgg cgagcagcag gacgtcctgg
     3541 aggcggtgaa ggcgggcgcg accggctacc tgctcaagtc cgccgcccgc gaggagttcc
     3601 tcgacgccgt ccggcggacc ggcgagggcg acgccgtctt cacccccggg ctcgccggtc
     3661 tcgtcctcgg cgagtaccgc cgcctcgccg ccgcccccgc ccgcgaggac gacgacgcgc
     3721 cgcggctgac cgagcgcgag accgaggtgc tgcgcctcgt cgcgaagggg ctgacgtaca
     3781 agcagatcgc gcagcgcctc gtcctgtccc atcggacggt gcagaaccac gtgcagaaca
     3841 ccctcggcaa gctccagctc cacaaccgcg tcgaactcgt ccgctacgcc atcgagaagg
     3901 gcctcgacga ggagcagtag cgcggggcga accgccgcgg cggcgcctgc gtctcatcgc
     3961 caaaggcgca tagcgacggg agcgcgtcat ggcggacgag atcctgggtc ccgctctggg
     4021 gttcctcgcg gcccacgtcc tggtcgggct cgtactgctg gtgatcctcc ggagaatgcg
     4081 ggccgcggtg ctgcggggaa ggcctcccgc gcgcgagctg cacccgtacg aggtcgccta
     4141 cctcaacggc ggcggccgcc acgcgatcgc cgcctcggtc acggcgctgc ggctggacgg
     4201 cgcggccgac gcctacgccg acggccggct gctggcacgc gaaccgtccg cggacgcccc
     4261 gcgcgcggcc ggaacgcccc tggacacggc ggttcactgg gcgatcgagc gtctgcgggc
     4321 cgagacgctc gcccagatca ccgccgaccc ggacgtgcgc gcggcgctcc ggcggctccg
     4381 cgacgggctc gccgcgcagg gcatggtgct cgggcaggcc gggcgccgcc ggctgcgcaa
     4441 cctccagctg gtcatgtacg ggtggatcct gctcggcttc gccgccttcg tcctgagcga
     4501 cgcgttcgac gggttcccgg ccaagctgat tccgctggcc gcgctggtcg gcctcggctt
     4561 cggagccctc gccctcggtc agggcgccga gcggacgcgg gagggcgagc gggccgtgga
     4621 gcggctcaag gagtcccact ccgccctcga cccggcgaac gggccctcct acaccgggct
     4681 gccggccccc gccgcgctga tgggagtggc gctgttcggc gcggccgcgc tcatggcgct
     4741 cgacccggtg ttcgtccaga ccgtcggact cggccggtac ctggagatgg ccggggtcgt
     4801 ggccgcctcc ggcggctcct cgggggcatc cggctcgtcg acctccttcg tgtgctcgac
     4861 gaccgccgcg gtgtgctcgt cctcctcctg cggtggcggt ggctcgtgcg gcggagggag
     4921 cggctgcggc ggggggagcg gatgtggagg aggtggcggt ggtggtggtg gcggctgcgg
     4981 cggcggagga gggagctgag atgcggctgg gcgtggggat cggctggcgc cccgagatcg
     5041 ccgggttcgt cggcgcgctg ccggggctgc gcttcaccga ggtgatcgcc gagtcggtgc
     5101 acccggcggg ccccgccacc gagctgctcg cgctgcgcga ccgcggcgcg gcgatcgtcc
     5161 cgcacggggt gcggctgtcg ctcggcggcg ccgagcccgt ggacgccgag cgcgtcgcgc
     5221 acctggccgc ctgcgccgag gcgctcggcg cgcccctggc gagcgagcac gtcgcgttcg
     5281 tccgcgcggg cggcgtcgag gcggggcacc tgctgccggt gccgcgcacc cgggccgcgc
     5341 tggacgcgct gaccgccaac atccgccgga cgcaggcgga gctgcccgtc ccgctggccg
     5401 tcgagcccat cgccgcgctc ttcgagtggc ccgacgccga gtacgccgag gccgacttcc
     5461 tgaccgagct gctggagcgc accggcgccc tcctgctgct cgacgtcgcc aacgtgtacg
     5521 ccaacgcgcg caaccggggc gaagaccccg cggacctcct cgaccgcctg cccctggacc
     5581 gcatcgccta ctgccacgtc gcgggcggct cgcaggaggg cgggcgctac cacgacacgc
     5641 acgccgccgc cgttcccgcc gaggtgctgg atctggtcgc ggagctgtgc gagcggcacc
     5701 gcccgcccgg cctcctgctg gagcgcgacg gccgctaccc gcccgcggcg gagctgcgcg
     5761 ccgagctgga cgccatcgcc gccgcctccg gactggcgcc cgtcacatga cgggggacgg
     5821 ggcatgagcg aattcgacgc cgggctcgcg gcggcgcagg aggcgctcgt gcgggccatg
     5881 acggcgggcg ggccgttgcc ggagggattc gacgccgagg cggtgggggc cgccgcgcac
     5941 ggaatcctgc tcaagcgggc gggcgaggtc gcccgcgcct ggccggcgct ggccgcctcg
     6001 tacggtacgt cctggaaggc ggccttcgcc gcatgggccg ccgagcgccc gacgcgcggc
     6061 tccttccggg acggctggga cttcgcgcgc gatcggggcc ggctcgccgg cgacgcggcg
     6121 cgggaactag ccctggccga agtccgatgg tcgtatgacg ggcaggaccc gccgcgcccg
     6181 cgcggcgcgg cggcgcggcg cgtgccgggg ggagcggcgg tggtcttccg ggggcgggtc
     6241 cgcgtcttcg gccggggcag gtccggctga ggccgccgtc cccgcgaacg aagtgccgcg
     6301 ggaccccgcc acgggctatg gtctagacca atggcggcgc gcggggacag gtggcggagg
     6361 cccgtgccgc cgcgtgcgac gatctagtct gtgaacgtgg accgagggag gagcccggtg
     6421 gcgggagaag gtgacggcat gcgcgtcgga gtgctgaccg gaggcgggga ctgccccggc
     6481 ctgaacgcgg tgatccgcgc ggtcgtccgc aagggcgtcc aggtctacgg ctacgagttc
     6541 gtcggcttcc gggccggctg gcgcgggccc ctcgaaggtg acaccgtggc cctcgacgtc
     6601 caggccgtgc gcgggatcct gccccgcggc gggacgatcc tcggctcctc gcggaccaac
     6661 ccggtcaagg tcgagggcgg cgtcgagcgg atcaaggaca acctcgcggg cctcggcgtg
     6721 gacgcgctga tcgccatcgg cggggaggac accctcggtg tcgcgagcga gctgtacgcc
     6781 aacggcgtca acgtcgtcgg cgtcccgaag acgatcgaca acgacctgaa cgcgaccgac
     6841 tacaccttcg ggttcgacac cgcggtgaac atcggcatgg aggccgtcga ccggctgcac
     6901 accaccgccg agagccacca ccgcgcgctg atctgcgagg tcatgggccg gcacgcgggc
     6961 tggatcgcgc tgcacgtcgg catggccgcc ggcgccaacg tcatcctcat ccccgagcgg
     7021 cccttcgaca tcgagaaggt ctgccagtac gtggagagcc ggttcaagac ccgctacgcc
     7081 ccgatcatcg tggtgtcgga gggcgcgcac cccgtcgacg ggcagatgca gctgcagacc
     7141 ggggagcggg acgcgttcgg gcacgtccgg ctcggcggca tcgggcaggc cctcgccgac
     7201 gagatcgaga agcgcaccgg caaggaggcc cgcgccaccg tgctcgggca catccagcgc
     7261 ggcggcaccc ccaccgcgtt cgaccgcgtc ctcgccaccc gcttcggtct ccaggcgatc
     7321 gacgcggtca aggacggcga ctacggcaag atggtcgccc tccagggcac cgacatcatc
     7381 cgcgtcggcc tcgacgaggc gacgaaggag ctcaagaccg tcccgctgga gcgctactcc
     7441 gaggccgagg tcttcttcgg ctgatcgccc ccgggcggga acagggaatg cgggtcgcgg
     7501 tgttggcttc tctgggccac actggaggtt ccctctttac ctgttcccgc cgggaggatc
     7561 acgcatgacc acgctcgaca ccgcgcccga cgccgcgccc ggcaccgcgg cggcggacgt
     7621 ccgggagcag tcgccggtca acggcgagcc gtgcgtgctg ctcaaactgg gcgaggtcgt
     7681 cctcaagggc aagaaccgcg agcagttcga gcgccgcctc gccgacaacg tgcgcaccgc
     7741 cgtccggccg atcgcccggg tggacgtcgt ccggcggcac ggcgtgttca tcgtccgccg
     7801 acccggtgct gatcttgcca ccatggagcg ggtcgcgcag cggatcaccg acgtcatggg
     7861 catcgtgtgg gcgcaccgcg cctggcaggt cggcaaggac ctcgccagcg tcgagcgcgc
     7921 cgccctggag ctcatggacg ggcggaccgg cacgttcgcc gtccggtccc ggcgccgcga
     7981 caagcggttc ccgatgacct ccaccgagct cgaccggcac atcggcacgc tcgtcaccgc
     8041 ccgctacggc cagcccgtcc ggctgaagaa ccccgagcac accctgtcga tcgaggtcga
     8101 ccgcgacgag gtgttcgtct actccggcgg cctgcccggg cagggcggcc tgcccgtcgg
     8161 gatgagcggc cgcggcctcg tgctgatgtc gggcggcatc gactcgcccg tcgccgcgta
     8221 ccggatgatg cgccgcggcc tgcgcgtcga ctacctgcac ttctccggca tgccgttcac
     8281 cggccccgag tcgatctaca aggcgtacgc gctggtccgc gagctggaca agttccagac
     8341 cgggtcccgg ctgttcgtcg tgccgttcgg caaggcgcag cagcagatca agtcctccgg
     8401 cgccgaccgg ctcgcggtga tcgcgcagcg gcggctgatg ctgcgcaccg gggaggtcct
     8461 cgcccggcgg ctgcgcggct ccgcgctgat caccggcgac gcgctcggcc aggtgtccag
     8521 ccagaccctc gccaacatca ccgcgctgga cgacgccgtc gagctgccca tcctgcggcc
     8581 cctcgtcggc atggacaagg tcgagatcat ggaccaggcg cgccgcatcc gcaccctgac
     8641 gatctccgag ctgcccgacg aggactgctg caccctgctc gcgccccgcc gcgccgagac
     8701 ccgggccaag atcggcgacc tgcggcagat cgagaagcgc ctcgacgtcg gcgagctcgc
     8761 cgaccagctc gccgcgtccg tccaggagca ccgccccgcc tacggcgacc cggccacctg
     8821 atcggccgct cgcgcccgcc gctggctagc gggcgtcgaa gtcagtcggg cgcggcggct
     8881 cggggccgtc cgggcccatc agggtgatcc gctcgatgcg gacgatgcgg aagcggcggc
     8941 ccgcgacggc gatgccgttg cggcggctgt cgtcctccat cagctccgcc gcctcggcga
     9001 accgggcgac gtcggtcgcg gcggggctct ccaccgccgg caccacgtgc ctgaagtagg
     9061 tggccagcga gtcgcgggcc tgctgcggcg cgtcgtacag gcgcccgacg ggccgccagc
     9121 gccgctcgcc gcgctcggcg acggtgaacg cgggcgccag cgcgaccggg gtgaggaacg
     9181 cgccggacgg ctcgttgccc gtggccgccg cggcgtccag cacctggcac agcagctcgg
     9241 cggtcgcgag gtcggacgcc gggccgtcgt ccggcagcag ctcgtgcggc cgggcgggcg
     9301 ggacgcggcc gcacgccggc agcgggtcca ggtccgtcgg gcggggcggc tcgggcccgt
     9361 ccgggccggt gcgggccagt tgctcgatcc gcacgatccg gaaccgtcgc ccgcccgcgg
     9421 tcacctcgtc ggccggctcg cgccgcagcg tccgggccgc ggccgccagg tcgcgcgccc
     9481 gcgcgccgga gcccggcgcg gcccggtcct ccagatggtc ggcgaggatc tcgcgcgcgc
     9541 cctgcggcag cgggtcgaac gggcacacga cctgccagcc gcccggaacc cgttccgcgg
     9601 cggtgtacac ggcgccgagg gccacaagtt ccgggtaggc ggcgagggcg cggcgggcgt
     9661 ccgccagtag caccgcggcg accgggtcga cgccgtccag gtcctccggg aacagccgcc
     9721 ccccggcgtc accatctccc gtcgtcatgc cggtcatcat cctcgccgcg ccgcgcccgg
     9781 ccaatggttc agaccatttc gttaccgccc tgtgcgcctt ccccgcggcc ctgtgcgcct
     9841 tccccgcggc cgcggcggcg gggcgccacg aggaccacca ggctcgtcgc cagcagcacg
     9901 aggccgccca gcgccgcgcc gcccagccgc tcgcccagca ggaccgtgcc gagcaccgcc
     9961 gcgaccgccg gctccgcgag cgtcagggtc gtcgccgtcg tcgcgggcgt cgtgcgcagg
    10021 ccccgggcga agagcaggta gccgccgccc gtggtgatca ccccgaggta gagcgcgatg
    10081 agcgcgccgc tcccggtgac aagccagccc gtcggtccgg cgagcagcac gggcaccagc
    10141 gggagggccg ccgtgccgaa cagcgccgcc gcgacggccc ggtcctgctc gccccgggtg
    10201 atgaggacgg acgccaccgt cgcgtacgcc gcgtacgcca gcccggacag cagggcgagc
    10261 ccgatgccga gcggctccgc gcccgcgtcc cggccgccgc cgacgagcag cgcgcagccc
    10321 gcgacggccc ccgccgtggc gagcgtccac cgcccggtcg gcgcggacct gcgcgccagc
    10381 agcccgatca cgccggtgaa cacgggcgcg ctgccgatcg tgacgatggt gccgaccgcg
    10441 acgccggtgc gcccggcggc gacgaagaac gccgtctggt acacggtgat ggcgaccgcc
    10501 ccgaggccga cgagcggcag gttgcggcgg tccgccacca ggtggcgcag ccccgcgccg
    10561 ccgcgcgtga gagcggcgag ggccagcagc accaggccgc cgatgatgat gcggatcgcg
    10621 gcgaccgagt acggggacgc gccgtccgcg aacgtgcgga cggtgccggt cgtcccccac
    10681 agggtcgccg ccagcaggac gtcggcgccg ccgcgccggg cacgggaccg cgaggacttt
    10741 tcaggcgcgc ggccaaggga ggtgttctgt gacacggaga gaggctcctg ggctcgaatc
    10801 ggaaggagag
//
