LOCUS       NZ_BMQY01000025         4576 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Couchioplanes caeruleus subsp. azureus strain JCM 3246 sequence25,
            whole genome shotgun sequence.
ACCESSION   NZ_BMQY01000025
VERSION     NZ_BMQY01000025.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Couchioplanes caeruleus subsp. azureus
  ORGANISM  Couchioplanes caeruleus subsp. azureus
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Couchioplanes.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 64X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,801
            CDSs (total)                      :: 6,731
            Genes (coding)                    :: 6,630
            CDSs (with protein)               :: 6,630
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 20 of 101
            Pseudo Genes (incomplete)         :: 93 of 101
            Pseudo Genes (internal stop)      :: 0 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:23:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4576
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMQY01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4576
                     /culture_collection="JCM:3246"
                     /db_xref="taxon:56428"
                     /mol_type="genomic DNA"
                     /organism="Couchioplanes caeruleus subsp. azureus"
                     /strain="JCM 3246"
                     /sub_species="azureus"
                     /submitter_seqid="sequence25"
                     /type_material="type strain of Couchioplanes caeruleus
                     subsp. azureus"
     gene            <1..>4576
                     /locus_tag="IEY99_RS33625"
     region          1..4576
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..4576
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4576
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4:>4576](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>4576
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS_motif       7..102
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0001"
                     /evalue="2.10E-17"
                     /label="PKSI-KR_m4"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="32"
                     /protein_start="0"
                     /score="55.7"
                     /tool="antismash"
                     /translation="MFSSVAGVFGAAGQGNYAAGNAYLDALAAHRH"
     CDS             5..>4576
                     /NRPS_PKS="Domain: PKS_PP (134-220). E-value: 1.5e-30.
                     Score: 97.4. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33625_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (241-661). E-value:
                     5.3e-180. Score: 590.8. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33625_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (739-1002). E-value: 4.4e-90.
                     Score: 294.0. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33625_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (1067-1225). E-value: 5.9e-62.
                     Score: 200.9. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33625_PKS_DH.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018828305.1"
                     /locus_tag="IEY99_RS33625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189208167.1"
                     /sec_met_domain="PP-binding (E-value: 3.6e-16, bitscore:
                     45.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 1.9e-226, bitscore:
                     737.9, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.5e-96, bitscore:
                     308.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 7.4e-92, bitscore: 294.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFSSVAGVFGAAGQGNYAAGNAYLDALAAHRHEQGLPATSLAWGP
                     WAQGMTETLSGADADRIARSGMPELSTDRGLALFDSAVAAGGPAYVTARLDLTAMRAHG
                     AVPALMRGLIRTRTRRTAAAGAVAQNLTQRLSGRSDAEQQDILLDLVRAQVAVVLGHTG
                     ADMIDPERAFQDLGFDSLTAIELRNRLGDASGMRLPATAIFDYPSARQLAGFLRDELLG
                     AHEAVLLPTAALPSVADDPIVIVGMACRFPGGVTSPEGLWELVRDGVDAIGDFPADRGW
                     DVDNLYSSDPDEAGTSYTRHGGFLHDAADFDPEFFGMSPREALATDAQQRLLLETTWEA
                     LERTGIDPATLKGSPTGVFAGVMYNDYAGTLDPAQFEGQLGSGSAGSIASGRVSYTFGF
                     EGPAVTVDTACSSSLVALHLAAQSLRSGECDLAVAGGVTVMSTPTTFVEFSRQGGLAPD
                     GRCKPFADAADGVGWAEGVGILVVERRSDALRHGHEILAVVRGSAVNSDGASNGLTAPN
                     GPSQQRVIRQALASAGLSAQDVDVVEAHGTGTRLGDPIEAQALFATYGQHRSQPLLLGS
                     VKSNIGHTQAAAGVAGVIKMVMAMRHGVVPRSLHVDVPSSHVDWRAGAVRVAAEQCVWP
                     GSGRRRAGVSSFGISGTNAHVILEQPDPTVPAQRSAPSGVVPWVVSAKSPDALREQLAR
                     LDAVDADPHDVGWSLATTRSRFAHRAVRIGDQLVQGTARPGRTAFMFTGQGSQRAGMGQ
                     GLYARYPVFAEAFDAVTAHLDIDWDDLDATGNAQPAIFAVEVALYRLLESWGVVPDVLL
                     GHSVGEIAAAHVAGVLCLEDACTLISARGRLMQALPSGGAMVAVEASEQDVLPLLTDGV
                     SLAAVNGPAAVVLSGVEEAVLAVVSRFERTKRLTVSHAFHSALMDPMLADFGRVVAGLD
                     LHTPTVPLVSNVTGRVETDLFTDPAYWVRHVRETVRFADGVEAATADRHLEVGPDAVLS
                     AITGGIAALRRERDEVTTLLTAVGTVFSQGHPVDWAAVIGRGHRTDLPTYPFQRQRFWP
                     APFAANVAGLGAAGLRELTHPLLGAAVRLPDDGGLIATGRLSIRTQPWLADHTVHGSVL
                     VPGTALVELALCAGAEVGTPGLEELTLAAPLVLPADDAVQIQISVGPQDDGRRSITVHS
                     EHDGQWTRHAQGFLTAATAAAPRDLAEWPPSGAQPIPVDGAYATFRAHGYSYGPAFQGL
                     RAVWAVGDDLYAEVALQGPAADDADGFGIHPALLDACLHAVILSSGAQQTMIPFAWNDV
                     TLHAAGAATVRVRLTKPGGDATTVEVADTTGRPVLSVRSMTGRPVTADQLAATTTPALY
                     TVEWQPSAARAQVIPSVVHEVVTPAGDVPSAVRALTGEVLEVLQRHLASDLPERLVVVT
                     RDAVAAEMPDVAQTPVWGLVRAAQAENPGRIVLVDSDGSVAVDTVDEPEFAVRAGTVLV
                     PRLVRTDVTADPPVLAGPVLITGGTGGIGAEIARRLVAENGVRSLLLTSRRGPDAPGAG
                     ELAA"
     misc_feature    68..70
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        409..666
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33625_PKS_PP.1"
                     /evalue="1.50E-30"
                     /label="IEY99_RS33625_PKS_PP.1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="220"
                     /protein_start="134"
                     /score="97.4"
                     /tool="antismash"
                     /translation="LSGRSDAEQQDILLDLVRAQVAVVLGHTGADMIDPERAFQDLGFD
                     SLTAIELRNRLGDASGMRLPATAIFDYPSARQLAGFLRDEL"
     misc_feature    617..619
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        730..1989
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33625_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="5.30E-180"
                     /label="IEY99_RS33625_PKS_KS.1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="661"
                     /protein_start="241"
                     /score="590.8"
                     /tool="antismash"
                     /translation="IVIVGMACRFPGGVTSPEGLWELVRDGVDAIGDFPADRGWDVDNL
                     YSSDPDEAGTSYTRHGGFLHDAADFDPEFFGMSPREALATDAQQRLLLETTWEALERTG
                     IDPATLKGSPTGVFAGVMYNDYAGTLDPAQFEGQLGSGSAGSIASGRVSYTFGFEGPAV
                     TVDTACSSSLVALHLAAQSLRSGECDLAVAGGVTVMSTPTTFVEFSRQGGLAPDGRCKP
                     FADAADGVGWAEGVGILVVERRSDALRHGHEILAVVRGSAVNSDGASNGLTAPNGPSQQ
                     RVIRQALASAGLSAQDVDVVEAHGTGTRLGDPIEAQALFATYGQHRSQPLLLGSVKSNI
                     GHTQAAAGVAGVIKMVMAMRHGVVPRSLHVDVPSSHVDWRAGAVRVAAEQCVWPGSGRR
                     RAGVSSFGISGTNAHVILEQP"
     aSModule        730..3681
                     /domains="nrpspksdomains_IEY99_RS33625_PKS_KS.1"
                     /domains="nrpspksdomains_IEY99_RS33625_PKS_AT.1"
                     /domains="nrpspksdomains_IEY99_RS33625_PKS_DH.1"
                     /incomplete
                     /locus_tags="IEY99_RS33625"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1207..1254
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0002"
                     /evalue="7.30E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="416"
                     /protein_start="400"
                     /score="21.0"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       1630..1674
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0003"
                     /evalue="2.60E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="556"
                     /protein_start="541"
                     /score="13.1"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       1729..1773
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0004"
                     /evalue="5.60E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="589"
                     /protein_start="574"
                     /score="12.3"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       1903..1941
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0005"
                     /evalue="4.90E+00"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="645"
                     /protein_start="632"
                     /score="0.8"
                     /tool="antismash"
                     /translation="VWPGSGRRRAGVS"
     CDS_motif       2221..2274
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0006"
                     /evalue="2.90E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="756"
                     /protein_start="738"
                     /score="16.6"
                     /tool="antismash"
                     /translation="FMFTGQGSQRAGMGQGLY"
     aSDomain        2224..3012
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33625_PKS_AT.1"
                     /evalue="4.40E-90"
                     /label="IEY99_RS33625_PKS_AT.1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="1002"
                     /protein_start="739"
                     /score="294.0"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="MFTGQGSQRAGMGQGLYARYPVFAEAFDAVTAHLDIDWDDLDATG
                     NAQPAIFAVEVALYRLLESWGVVPDVLLGHSVGEIAAAHVAGVLCLEDACTLISARGRL
                     MQALPSGGAMVAVEASEQDVLPLLTDGVSLAAVNGPAAVVLSGVEEAVLAVVSRFERTK
                     RLTVSHAFHSALMDPMLADFGRVVAGLDLHTPTVPLVSNVTGRVETDLFTDPAYWVRHV
                     RETVRFADGVEAATADRHLEVGPDAVLSAITGGIAALRRER"
     CDS_motif       2362..2409
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0007"
                     /evalue="3.20E-03"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="801"
                     /protein_start="785"
                     /score="10.9"
                     /tool="antismash"
                     /translation="AQPAIFAVEVALYRLL"
     CDS_motif       2419..2541
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0008"
                     /evalue="3.50E-23"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="845"
                     /protein_start="804"
                     /score="73.9"
                     /tool="antismash"
                     /translation="GVVPDVLLGHSVGEIAAAHVAGVLCLEDACTLISARGRLMQ"
     CDS_motif       2626..2664
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0009"
                     /evalue="1.90E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="886"
                     /protein_start="873"
                     /score="11.6"
                     /tool="antismash"
                     /translation="AAVNGPAAVVLSG"
     CDS_motif       2872..2907
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0010"
                     /evalue="4.40E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="967"
                     /protein_start="955"
                     /score="16.8"
                     /tool="antismash"
                     /translation="YWVRHVRETVRF"
     aSDomain        3208..3681
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33625_PKS_DH.1"
                     /evalue="5.90E-62"
                     /label="IEY99_RS33625_PKS_DH.1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="1225"
                     /protein_start="1067"
                     /score="200.9"
                     /tool="antismash"
                     /translation="HPLLGAAVRLPDDGGLIATGRLSIRTQPWLADHTVHGSVLVPGTA
                     LVELALCAGAEVGTPGLEELTLAAPLVLPADDAVQIQISVGPQDDGRRSITVHSEHDGQ
                     WTRHAQGFLTAATAAAPRDLAEWPPSGAQPIPVDGAYATFRAHGYSYGPAFQGL"
     CDS_motif       3292..3366
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0011"
                     /evalue="1.60E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="1120"
                     /protein_start="1095"
                     /score="33.4"
                     /tool="antismash"
                     /translation="WLADHTVHGSVLVPGTALVELALCA"
     CDS_motif       4447..4542
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33625_0012"
                     /evalue="4.60E-09"
                     /label="PKSI-KR_m1"
                     /locus_tag="IEY99_RS33625"
                     /protein_end="1512"
                     /protein_start="1480"
                     /score="28.9"
                     /tool="antismash"
                     /translation="LITGGTGGIGAEIARRLVAENGVRSLLLTSRR"
ORIGIN
        1 tcatgttctc ctccgtcgcg ggcgtcttcg gcgctgccgg acagggcaac tacgccgccg
       61 gcaacgctta cctggacgcc ctcgccgcgc accgccacga gcagggcctg cccgccacct
      121 cactcgcctg gggcccctgg gcgcagggca tgaccgagac cctcagcggc gccgacgccg
      181 accgcatcgc ccgctccggc atgcccgaac tcagcacgga tcgcggcctc gccctgttcg
      241 acagtgccgt cgcggccggg gggccggcgt acgtcacggc tcgcctcgac ctgaccgcga
      301 tgcgcgccca cggcgccgtg cccgcattga tgcgtggcct catccgtacc cgtacccgcc
      361 gtaccgcggc ggccggtgcc gtcgcccaga acctcactca gcgtctgtcg ggacgttccg
      421 acgccgagca gcaggacatc ctgctcgatc tggttcgtgc ccaggtcgct gtggtcctcg
      481 gccacaccgg tgccgacatg atcgacccgg aacgcgcctt ccaggacctc gggttcgact
      541 cgctcaccgc gatcgagctg cgcaaccggc tcggcgacgc ctccgggatg cggctgcccg
      601 ccaccgcgat cttcgattac ccgagtgccc ggcaactcgc cggattcctg cgggacgagc
      661 tgctcggcgc ccacgaagcc gtgctgctgc cgaccgcggc gctcccgtcc gtggccgacg
      721 atccgatcgt catcgtgggt atggcctgcc ggttcccggg tggcgtcacc tcgcccgagg
      781 gactgtggga gctggtccgc gacggcgtcg acgcgatcgg cgacttcccg gccgaccgcg
      841 gctgggacgt ggacaacctc tacagcagcg acccggacga ggcaggcacc tcgtacaccc
      901 gccacggtgg cttcctgcac gatgccgccg acttcgaccc ggagttcttc gggatgagtc
      961 cgcgcgaggc gctggccacc gacgcccagc aacggctgct gctggagacc acctgggagg
     1021 cgctggagcg gacgggcatc gacccggcca cgctcaaggg cagtcccacc ggcgtgttcg
     1081 ccggtgtcat gtacaacgac tacgccggca cgctcgaccc cgctcagttc gaggggcaac
     1141 tcggcagcgg cagcgccggc agcatcgcct cgggccgcgt ctcctacacg ttcgggttcg
     1201 agggtccggc ggtcaccgtc gacacggcgt gttcgtcgtc gctggtggcc ctgcatctgg
     1261 cggcccagtc gctgcggtcg ggggagtgcg acctcgctgt cgcgggcggt gtgacagtca
     1321 tgtccacgcc gaccacgttc gtcgagttct cccgccaggg cgggttggcg cccgacgggc
     1381 ggtgcaagcc gttcgccgat gcggcggacg gtgtgggctg ggccgaaggt gtcggcatcc
     1441 tcgtcgtcga gcggcgctcg gacgcgctgc ggcacggtca cgagatcctc gccgtggtgc
     1501 ggggttcggc ggtgaattcc gatggtgcgt cgaatggtct gacggcgccc aacgggccgt
     1561 cgcagcagcg ggtgatccgg caggcgttgg cctcggccgg gctgtcggcg caggacgtcg
     1621 atgtggtgga ggcgcacggg actggtacga ggttgggtga tccgatcgag gcgcaggcgt
     1681 tgttcgccac gtacgggcag catcgtagtc agccgctcct tctcggctcg gtgaagtcga
     1741 acatcggtca tacgcaggcg gcggcgggtg tcgctggtgt gatcaagatg gtgatggcga
     1801 tgcggcatgg tgtggtgccg cggtcgttgc atgtggatgt gccgtcttcg catgtggatt
     1861 ggcgtgcggg tgcggtgcgg gtggctgcgg agcagtgtgt gtggccgggg tcgggtcggc
     1921 gtcgtgcggg ggtgtcgtcg ttcggtatct cggggacgaa cgcccacgtc atcctcgaac
     1981 agccggatcc cacggttccc gcgcagcggt ccgccccgag cggggtcgtg ccgtgggtcg
     2041 tgtcggccaa gtcgccggac gccctgcgcg agcagctcgc gcgactcgac gccgtggacg
     2101 ccgacccgca cgacgtcggc tggtccctgg ccaccaccag gagcagattc gcgcaccgcg
     2161 cggtccgcat cggcgaccag ctcgttcagg gcaccgcccg gccgggccgc accgccttca
     2221 tgttcaccgg tcagggttct cagcgcgcgg ggatgggcca gggtttgtac gcccggtatc
     2281 cggtgttcgc cgaggccttc gacgcggtga ccgcacatct ggacatcgac tgggacgacc
     2341 tggacgccac cggtaacgcc cagccggcca tcttcgccgt cgaggtcgcc ctctaccggc
     2401 tcctggaatc gtggggtgtc gtcccggacg tgttgctcgg ccactcggtc ggggagatcg
     2461 ccgccgcgca cgtggccggg gtcctctgtc tggaagacgc ctgcacgctg atctccgctc
     2521 gcggccggct catgcaggcg cttccgtccg gcggcgccat ggtcgccgtc gaggcgtccg
     2581 agcaggacgt gctgccgctg ctgaccgacg gtgtctcgct cgccgccgtc aacggtccgg
     2641 ccgcggtcgt gctgtccggc gtcgaggagg cggtcctcgc cgtggtctcc cgcttcgagc
     2701 ggaccaagcg gctcacggtc agccacgcct tccactccgc gctcatggac ccgatgctgg
     2761 ccgacttcgg ccgggtcgtc gccggtctcg acctgcacac gccgaccgtg ccgctcgtct
     2821 ccaacgtcac cggacgggtg gagaccgacc tgttcaccga cccggcctac tgggtgcggc
     2881 acgtgcggga gacggtgcgc ttcgccgacg gggtcgaggc cgccaccgcc gaccggcacc
     2941 tcgaggtcgg cccggacgcc gtgctgtccg cgatcaccgg cggcattgcg gccctgcgcc
     3001 gggaacgcga cgaggtcacc accctgctga ccgccgtcgg taccgtcttc agccagggtc
     3061 acccggtcga ctgggctgcc gtcatcggcc gcggccaccg caccgacctg cccacctacc
     3121 cgttccagcg gcagcgcttc tggcccgccc cgttcgcggc gaacgtcgca ggtctcggcg
     3181 cggccggcct gcgcgagctc acccatccgc tgctcggcgc ggcggtacgc ctgcccgacg
     3241 acggcggtct gatcgccacc gggcgcctca gcatccggac gcagccctgg ctggccgacc
     3301 acaccgtgca cggctccgtg ctcgttccgg gcaccgcctt ggtcgagctg gccctgtgcg
     3361 ccggcgccga ggtgggcacc cccgggctgg aggagctgac cctggccgcg cccctggtgc
     3421 tgccggccga cgacgcggtg cagatccaga tcagcgtcgg cccgcaggac gacggtcgcc
     3481 gcagcatcac cgtgcactcc gagcacgacg gccagtggac ccgccacgcg cagggcttcc
     3541 tcaccgcggc caccgcggcc gccccgcgcg acctggccga gtggccgcca tccggcgcgc
     3601 agccgatacc ggtggacggc gcgtacgcca cgttccgcgc gcacgggtac tcgtacgggc
     3661 ctgccttcca aggcctgcgc gccgtctggg cggtcggcga cgacctgtac gccgaggtcg
     3721 ccctgcaagg gccggccgcc gacgacgccg acggcttcgg catccacccg gccctgctgg
     3781 acgcctgcct gcacgcggtc attctcagca gcggcgcaca gcagacgatg atccccttcg
     3841 cctggaacga cgtcaccctg cacgccgccg gtgctgccac ggtccgggtg cggctcacca
     3901 aaccgggcgg cgacgccacc accgtcgagg tcgcggacac caccggccgt ccggtgctgt
     3961 cggtccgctc gatgaccggc cgtccggtca ccgcggacca gctcgccgcc accaccacac
     4021 ccgcgctcta caccgtcgag tggcagccct ccgcagcccg cgcccaggtg atcccgtcgg
     4081 tggtgcacga ggtcgtcacc cccgcgggtg acgtgccgtc cgcggtgcgg gcgttgaccg
     4141 gtgaggttct ggaggtcttg cagcggcatc tggcttcgga tctgccggag cggttggtcg
     4201 tggtgacccg cgatgcggtg gccgcggaga tgccggatgt ggcgcagacg ccggtgtggg
     4261 gtctggtccg ggccgcgcag gcggagaacc cgggccgcat cgtgctggtg gattccgacg
     4321 gtagcgtcgc ggtggacacc gtggacgagc ccgagttcgc cgttcgcgcg ggcactgtgc
     4381 tggtgccacg gctggtccgc accgatgtca ccgccgaccc gccggttctc gccggcccgg
     4441 tcctgatcac cggtggcacc ggcggcatcg gcgccgagat cgcccgccgg ctcgtcgccg
     4501 agaacggcgt gcgcagcctg ctgctcacca gccgtcgcgg gccggacgcg ccgggcgccg
     4561 gtgagctggc ggccga
//
