LOCUS       NZ_BMQY01000023         7010 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Couchioplanes caeruleus subsp. azureus strain JCM 3246 sequence23,
            whole genome shotgun sequence.
ACCESSION   NZ_BMQY01000023
VERSION     NZ_BMQY01000023.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Couchioplanes caeruleus subsp. azureus
  ORGANISM  Couchioplanes caeruleus subsp. azureus
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Couchioplanes.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 64X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,801
            CDSs (total)                      :: 6,731
            Genes (coding)                    :: 6,630
            CDSs (with protein)               :: 6,630
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 20 of 101
            Pseudo Genes (incomplete)         :: 93 of 101
            Pseudo Genes (internal stop)      :: 0 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:23:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7010
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMQY01000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7010
                     /culture_collection="JCM:3246"
                     /db_xref="taxon:56428"
                     /mol_type="genomic DNA"
                     /organism="Couchioplanes caeruleus subsp. azureus"
                     /strain="JCM 3246"
                     /sub_species="azureus"
                     /submitter_seqid="sequence23"
                     /type_material="type strain of Couchioplanes caeruleus
                     subsp. azureus"
     gene            <1..>7010
                     /locus_tag="IEY99_RS33605"
     region          1..7010
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..7010
                     /SMILES="C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7010
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4:>7010](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>7010
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS_motif       7..102
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0001"
                     /evalue="3.80E-17"
                     /label="PKSI-KR_m4"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="32"
                     /protein_start="0"
                     /score="54.8"
                     /tool="antismash"
                     /translation="MFSSVAGVFGAAGQGNYAAANSALDALAEHRH"
     CDS             5..>7010
                     /NRPS_PKS="Domain: ACP (140-211). E-value: 3.2e-32. Score:
                     102.7. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (232-652). E-value:
                     2.1e-179. Score: 588.8. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (730-986). E-value: 2.4e-91.
                     Score: 298.2. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (1055-1219). E-value: 1.1e-61.
                     Score: 200.0. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1457-1622). E-value: 7.8e-58.
                     Score: 187.4. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1710-1796). E-value: 9.9e-31.
                     Score: 97.9. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (1817-2237). E-value:
                     8.4e-182. Score: 596.7. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33605_PKS_KS.2"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018828305.1"
                     /locus_tag="IEY99_RS33605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189208166.1"
                     /sec_met_domain="PP-binding (E-value: 8.2e-17, bitscore:
                     47.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 9.1e-98, bitscore:
                     312.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 3.9e-93, bitscore: 298.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.3e-58, bitscore:
                     183.9, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 4e-226, bitscore: 736.8,
                     seeds: 217, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFSSVAGVFGAAGQGNYAAANSALDALAEHRHALGLPATSMAWGP
                     WARDGGMIGTLADVDVARIARNGLPELSAAEGTALFDAALAAGRPVVAPVRLDLAAIQA
                     SGTVPPLLRGLIRPRTRRAVAASAAAVRADLGDLVSTVRAEVATVLGHQSAAGIDPQRA
                     FQDLGFDSLTAVELRNRLGAVTGLRLSATVVFDYPSVAALAGHLREELRGGAPDERIPV
                     AALPPVADDPIVIVGMACRFPGGVTSPEGLWELVRDGVDAIGDFPADRGWDLDRLYSAD
                     RTVAGTSYTRHGGFLHDAARFDPEFFGMSPREALATDAQQRLLLETTWEAVERTGIDPL
                     TLRGSRTGVFAGVMYTDYGNLLTEDSFEGFRGNGSAASIASGRVSYAFGFEGPAVTVDT
                     ACSSSLVALHLAAQSLRSGECDLALAGGVTVMSTPTTFVEFSRQGGLAPDGRCKPFADA
                     ADGVGWAEGVGLVVLERLSDARANGHEVLAVVRGSAVNSDGASNGLTAPNGPSQQRVIR
                     QALASAGLSAQDVDVVEAHGTGTSLGDPIEAQALVAAYGRDRDVPLLLGSVKSNIGHTQ
                     AAAGVAGVIKMVMAMRHGVVPRSLHVDVPSSHVDWRAGAVRVAAEQCVWPGSGRRRAGV
                     SSFGISGTNAHVILEQSTEAPAAVVEHGDAVLPLVLSAKSAGALSELIDRLGTLDAHPA
                     DVAAALARRSTSFPHRAVKVGDILLEGVATDTPLAMMFTGQGSQRAGMGRDLYARYPVF
                     AGAFDAVTAHLDIDWEDLDATGNAQPAIFAVEVALYRLLESWGVVPDVLLGHSVGEIAA
                     AHLAGVLSLEDACTLISARGRLMQELPAGGAMVAVEASEGDVRAALVDGVDIAAVNGPD
                     AVVLSGAAAAVEAVAAGFRRSKRLTVSHAFHSALMDPMLEAFRQVVDGLTLHRPQIPLV
                     SNVTGRIETDLFTDPAYWVRHVRETVRFADAVGAAGDVWLLEVGPDAVLSAMAGGSPTL
                     RRDRDEVTTLLTAVGALYTQGRSVDWAAVVDSGRRIDLPTYPFQHQRFWPASMILGGAA
                     PGLDAAGHPLLSGVTTLPGSDALLFTGRLSTSAQPWLADHEMQGRVMVPGTALVELALH
                     AGAEAGTPGLDQLTLTAPLVLPEHGGTLQVQVWAGAPDGSGRRAVTVHSRPDGDPDAVW
                     TEHATGLLGMAPTPVTFDAAAWPPPGARPVGIDGLYDRLADGGFGYGPAFQGLRGAWRD
                     GEHMYAEVTLPADAGTERYGLHPALFDTALHALLLDTADSPAGIPFAWHGVALHAAGAT
                     DARVRLTRNREGFAIALADPTGAPIASADSLLVRAPQPRTATDSLYRVDWVPVTLPAAT
                     AAATVVVVEAGEEPRTAVLEALARVQEFLAGADPEQRLVFVTRPGDLAGAAVSGLIRSA
                     QSEHPGRFGLIETGGDVPDDALAADEPRLALHDGVATVPRLVRVPAAPVAADPLSGTVL
                     ITGGTGGLGALIARHLAAQGVTDLVLVSRRGPDAPGAGELPGRVVACDVADRSAVDALV
                     AGLPELCAVIHAAGVLDDGVLASLDAERVDAVLAPKITAAWNLHAATRDRDLTAFVLFS
                     SIAGLFGNPGQASYAAGNAYLDALAETRRAAGLPGTSLAWGPWVRSGGMTAELTDGDVA
                     RMTRAGLPPLTQEQGLALFDAALAIPAATLVPVRLDLGALRARGDVPPLLRGLVRGPSR
                     RAAAVGSAALVRRLAALGPQERTEAVLDVVRTQIAQVLGHTGTDAVEPGKAFQDLGFDS
                     LTAIELRNRLGAATGLGLSATLVFDYPNAAALAAHLRDELLGTVAETLTRTPVVSPVSD
                     DPVVIVGMACRYPGGVTSPEDLWRLVHDGVDAVGDFPSDRGWDVDSLYSTDRNALGTSY
                     TRWGGFLDDAAGFDPEFFGMSPREALATDSQQRLLLESVWEAVERAGIDPHTLRGSQTG
                     VFAGVMYNDYANLLGGGQYEGYQGSGSAGSIASGRVSYTFGFEGPAVTVDTACSSSLVA
                     LHLGAQALRAGECDLALAGGVTVMSTPSTFVEFSRQGGLSADGRCKPFADAADGVGWGE
                     GVGVLVLERLSDARRHGHQILAVVRGSAVNSDGASNGLTAPNGPSQQRVIRQAVANAGL
                     SFRDVDVVEAHGTGTTLGDPIEAQALLATYGRDRDEPLLLGSVKSNIGHTQAAAGVAGV
                     IKMVVAMRHGVVPRTLHVDAPSSHVDWEAGAVALARENTPWPDSPKRRAGVSSFGISGT
                     NAHVILEQPDPPAVPAERPATDEVVPLVVSAMSAGALRDQVARLADVAGDPRDIGWSLA
                     TTRSEFAHRAVRLGPTLIEGEARTRRTAFMFTGQGSQRAGMGRGLYERF"
     aSDomain        427..639
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_ACP.1"
                     /evalue="3.20E-32"
                     /label="IEY99_RS33605_ACP.1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="211"
                     /protein_start="140"
                     /score="102.7"
                     /tool="antismash"
                     /translation="TVRAEVATVLGHQSAAGIDPQRAFQDLGFDSLTAVELRNRLGAVT
                     GLRLSATVVFDYPSVAALAGHLREEL"
     aSDomain        703..1962
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.10E-179"
                     /label="IEY99_RS33605_PKS_KS.1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="652"
                     /protein_start="232"
                     /score="588.8"
                     /tool="antismash"
                     /translation="IVIVGMACRFPGGVTSPEGLWELVRDGVDAIGDFPADRGWDLDRL
                     YSADRTVAGTSYTRHGGFLHDAARFDPEFFGMSPREALATDAQQRLLLETTWEAVERTG
                     IDPLTLRGSRTGVFAGVMYTDYGNLLTEDSFEGFRGNGSAASIASGRVSYAFGFEGPAV
                     TVDTACSSSLVALHLAAQSLRSGECDLALAGGVTVMSTPTTFVEFSRQGGLAPDGRCKP
                     FADAADGVGWAEGVGLVVLERLSDARANGHEVLAVVRGSAVNSDGASNGLTAPNGPSQQ
                     RVIRQALASAGLSAQDVDVVEAHGTGTSLGDPIEAQALVAAYGRDRDVPLLLGSVKSNI
                     GHTQAAAGVAGVIKMVMAMRHGVVPRSLHVDVPSSHVDWRAGAVRVAAEQCVWPGSGRR
                     RAGVSSFGISGTNAHVILEQS"
     aSModule        703..5394
                     /complete
                     /domains="nrpspksdomains_IEY99_RS33605_PKS_KS.1"
                     /domains="nrpspksdomains_IEY99_RS33605_PKS_AT.1"
                     /domains="nrpspksdomains_IEY99_RS33605_PKS_DH.1"
                     /domains="nrpspksdomains_IEY99_RS33605_PKS_KR.1"
                     /domains="nrpspksdomains_IEY99_RS33605_PKS_PP.1"
                     /locus_tags="IEY99_RS33605"
                     /monomer_pairings="mal -> ccmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1180..1227
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0002"
                     /evalue="1.20E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="407"
                     /protein_start="391"
                     /score="20.4"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       1603..1647
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0003"
                     /evalue="4.60E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="547"
                     /protein_start="532"
                     /score="12.4"
                     /tool="antismash"
                     /translation="VEAHGTGTSLGDPIE"
     CDS_motif       1702..1746
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0004"
                     /evalue="9.50E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="580"
                     /protein_start="565"
                     /score="11.7"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       1876..1911
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0005"
                     /evalue="8.50E+00"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="635"
                     /protein_start="623"
                     /score="0.1"
                     /tool="antismash"
                     /translation="VWPGSGRRRAGV"
     CDS_motif       1972..2046
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0006"
                     /evalue="4.70E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="680"
                     /protein_start="655"
                     /score="-2.6"
                     /tool="antismash"
                     /translation="PAAVVEHGDAVLPLVLSAKSAGALS"
     aSDomain        2197..2964
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_PKS_AT.1"
                     /evalue="2.40E-91"
                     /label="IEY99_RS33605_PKS_AT.1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="986"
                     /protein_start="730"
                     /score="298.2"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="MFTGQGSQRAGMGRDLYARYPVFAGAFDAVTAHLDIDWEDLDATG
                     NAQPAIFAVEVALYRLLESWGVVPDVLLGHSVGEIAAAHLAGVLSLEDACTLISARGRL
                     MQELPAGGAMVAVEASEGDVRAALVDGVDIAAVNGPDAVVLSGAAAAVEAVAAGFRRSK
                     RLTVSHAFHSALMDPMLEAFRQVVDGLTLHRPQIPLVSNVTGRIETDLFTDPAYWVRHV
                     RETVRFADAVGAAGDVWLLEVGPDAVLSAMAGGS"
     CDS_motif       2200..2247
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0007"
                     /evalue="8.60E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="747"
                     /protein_start="731"
                     /score="18.2"
                     /tool="antismash"
                     /translation="FTGQGSQRAGMGRDLY"
     CDS_motif       2392..2514
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0008"
                     /evalue="3.10E-24"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="836"
                     /protein_start="795"
                     /score="77.3"
                     /tool="antismash"
                     /translation="GVVPDVLLGHSVGEIAAAHLAGVLSLEDACTLISARGRLMQ"
     CDS_motif       2599..2637
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0009"
                     /evalue="7.70E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="877"
                     /protein_start="864"
                     /score="12.8"
                     /tool="antismash"
                     /translation="AAVNGPDAVVLSG"
     CDS_motif       2779..2811
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0010"
                     /evalue="3.20E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="935"
                     /protein_start="924"
                     /score="10.3"
                     /tool="antismash"
                     /translation="PQIPLVSNVTG"
     CDS_motif       2845..2880
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0011"
                     /evalue="7.10E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="958"
                     /protein_start="946"
                     /score="16.1"
                     /tool="antismash"
                     /translation="YWVRHVRETVRF"
     aSDomain        3172..3663
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_PKS_DH.1"
                     /evalue="1.10E-61"
                     /label="IEY99_RS33605_PKS_DH.1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1219"
                     /protein_start="1055"
                     /score="200.0"
                     /tool="antismash"
                     /translation="HPLLSGVTTLPGSDALLFTGRLSTSAQPWLADHEMQGRVMVPGTA
                     LVELALHAGAEAGTPGLDQLTLTAPLVLPEHGGTLQVQVWAGAPDGSGRRAVTVHSRPD
                     GDPDAVWTEHATGLLGMAPTPVTFDAAAWPPPGARPVGIDGLYDRLADGGFGYGPAFQG
                     L"
     CDS_motif       3256..3330
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0012"
                     /evalue="4.30E-08"
                     /label="PKSI-DH_m2"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1108"
                     /protein_start="1083"
                     /score="25.7"
                     /tool="antismash"
                     /translation="WLADHEMQGRVMVPGTALVELALHA"
     aSDomain        4378..4872
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_PKS_KR.1"
                     /evalue="7.80E-58"
                     /label="IEY99_RS33605_PKS_KR.1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1622"
                     /protein_start="1457"
                     /score="187.4"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLITGGTGGLGALIARHLAAQGVTDLVLVSRRGPDAPGAGELP
                     GRVVACDVADRSAVDALVAGLPELCAVIHAAGVLDDGVLASLDAERVDAVLAPKITAAW
                     NLHAATRDRDLTAFVLFSSIAGLFGNPGQASYAAGNAYLDALAETRRAAGLPGTSLAWG
                     PW"
     CDS_motif       4381..4479
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0013"
                     /evalue="1.10E-12"
                     /label="PKSI-KR_m1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1491"
                     /protein_start="1458"
                     /score="40.5"
                     /tool="antismash"
                     /translation="TVLITGGTGGLGALIARHLAAQGVTDLVLVSRR"
     CDS_motif       4726..4830
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0014"
                     /evalue="9.60E-21"
                     /label="PKSI-KR_m4"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1608"
                     /protein_start="1573"
                     /score="66.4"
                     /tool="antismash"
                     /translation="AFVLFSSIAGLFGNPGQASYAAGNAYLDALAETRR"
     aSDomain        5137..5394
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_PKS_PP.1"
                     /evalue="9.90E-31"
                     /label="IEY99_RS33605_PKS_PP.1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1796"
                     /protein_start="1710"
                     /score="97.9"
                     /tool="antismash"
                     /translation="LAALGPQERTEAVLDVVRTQIAQVLGHTGTDAVEPGKAFQDLGFD
                     SLTAIELRNRLGAATGLGLSATLVFDYPNAAALAAHLRDEL"
     aSDomain        5458..6717
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33605_PKS_KS.2"
                     /domain_subtypes="Modular-KS"
                     /evalue="8.40E-182"
                     /label="IEY99_RS33605_PKS_KS.2"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="2237"
                     /protein_start="1817"
                     /score="596.7"
                     /tool="antismash"
                     /translation="VVIVGMACRYPGGVTSPEDLWRLVHDGVDAVGDFPSDRGWDVDSL
                     YSTDRNALGTSYTRWGGFLDDAAGFDPEFFGMSPREALATDSQQRLLLESVWEAVERAG
                     IDPHTLRGSQTGVFAGVMYNDYANLLGGGQYEGYQGSGSAGSIASGRVSYTFGFEGPAV
                     TVDTACSSSLVALHLGAQALRAGECDLALAGGVTVMSTPSTFVEFSRQGGLSADGRCKP
                     FADAADGVGWGEGVGVLVLERLSDARRHGHQILAVVRGSAVNSDGASNGLTAPNGPSQQ
                     RVIRQAVANAGLSFRDVDVVEAHGTGTTLGDPIEAQALLATYGRDRDEPLLLGSVKSNI
                     GHTQAAAGVAGVIKMVVAMRHGVVPRTLHVDAPSSHVDWEAGAVALARENTPWPDSPKR
                     RAGVSSFGISGTNAHVILEQP"
     CDS_motif       5935..5982
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0015"
                     /evalue="1.20E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="1992"
                     /protein_start="1976"
                     /score="20.4"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       6358..6402
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0016"
                     /evalue="5.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="2132"
                     /protein_start="2117"
                     /score="12.3"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       6457..6501
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0017"
                     /evalue="9.50E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="2165"
                     /protein_start="2150"
                     /score="11.7"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       6952..7005
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33605_0018"
                     /evalue="1.60E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IEY99_RS33605"
                     /protein_end="2333"
                     /protein_start="2315"
                     /score="17.3"
                     /tool="antismash"
                     /translation="FMFTGQGSQRAGMGRGLY"
ORIGIN
        1 tcatgttctc ctccgtcgcg ggcgtcttcg gcgctgccgg acagggcaac tacgccgccg
       61 ccaacagcgc cctcgacgcc ctcgccgagc accgccacgc cctcgggctc cccgccacct
      121 cgatggcctg gggaccctgg gcgcgcgacg gcggcatgat cggcaccctc gccgacgtgg
      181 acgtggcccg catcgcccgc aacggcctgc cggaactcag cgccgccgag ggcaccgccc
      241 tgttcgacgc cgccctcgcc gccggccgtc cggtcgtcgc cccggtgcgc ctcgacctcg
      301 ccgcgatcca ggcgtccgga accgtcccgc cgctgctgcg cggcctgatc cgtccgcgta
      361 cccgccgggc cgtcgccgcg agcgctgccg ccgtccgggc ggacctcggc gacctggtga
      421 gcacggtccg cgccgaggtc gccaccgtcc tcggtcacca gagcgcggcc ggcatcgatc
      481 cgcagcgcgc cttccaggac ctgggcttcg actcgctcac cgccgtcgag ctgcgcaacc
      541 gcctcggtgc cgtcaccggg ctccggctgt ccgccaccgt cgtcttcgac tacccgtcgg
      601 tcgccgccct cgccgggcat ctgcgcgagg aactgcgggg tggggcaccg gatgagcgga
      661 tcccggtcgc cgccctgccg cccgtggccg acgatccgat cgtcatcgtg ggtatggcct
      721 gccggttccc gggcggcgtc acctcgcccg agggactgtg ggagctggtc cgcgacggcg
      781 tcgacgcgat cggcgacttc ccggccgacc gcggctggga cctcgaccgc ctctacagcg
      841 cggaccgtac ggtcgccggt acctcgtaca cccgccacgg tggcttcctg cacgacgccg
      901 cccggtttga tccggagttc ttcgggatga gtccgcgcga ggcgctggcc accgacgccc
      961 agcaacggct gctgctggag accacgtggg aggccgtgga gcggacgggc atcgatccgc
     1021 tgaccctgcg cggcagccgg accggtgtct tcgccggcgt catgtacacc gactacggca
     1081 acctgctcac cgaggactcg ttcgagggct tccgcggcaa cgggagcgcg gcgagcatcg
     1141 cctccggacg ggtctcgtac gccttcgggt tcgagggtcc ggcggtcacc gtcgacacgg
     1201 cgtgttcgtc gtcgctggtg gccctgcatc tggcggccca gtcgctgcgg tcgggggagt
     1261 gcgatctggc tctcgcgggt ggtgtgacag tcatgtccac gccgaccacg ttcgtcgagt
     1321 tctcccgcca gggtgggttg gcgcccgacg ggcggtgcaa gccgttcgcc gatgcggcgg
     1381 acggtgtggg ctgggccgag ggcgtggggc tggtggtcct ggaacggctt tcggatgcgc
     1441 gggccaacgg tcacgaggtc ctcgccgtgg tgcggggttc ggcggtgaat tccgatggtg
     1501 cgtcgaatgg tctgacggcg cccaacgggc cgtcgcagca gcgggtgatc cggcaggcgt
     1561 tggcctcggc cgggctgtcg gcgcaggacg tcgatgtggt ggaggcgcac gggactggta
     1621 cgtcgttggg tgatccgatc gaggcgcagg cgttggtggc ggcgtatggc cgggatcgtg
     1681 atgtgccgtt gttgctgggc tcggtgaagt cgaacatcgg tcatacgcag gcggcggcgg
     1741 gtgtcgctgg tgtgatcaag atggtgatgg cgatgcggca tggtgtggtg ccgcggtcgt
     1801 tgcatgtgga tgtgccgtct tcgcatgtgg attggcgtgc gggtgcggtg cgggtggctg
     1861 cggagcagtg tgtgtggccg gggtcgggtc ggcgtcgtgc gggggtgtcg tcgttcggta
     1921 tctcggggac gaacgcccac gtcatcctcg aacagtccac cgaagcgccg gccgcggtgg
     1981 tcgagcacgg tgacgccgtc ctcccactcg tgctgtccgc caagtcggcc ggcgccctgt
     2041 ccgagctgat cgaccggctc ggcaccctcg acgcgcaccc ggccgacgtc gccgccgctc
     2101 tggcccgccg atcgacgtcg ttcccgcatc gcgcggtcaa ggtcggcgac atcctcctcg
     2161 aaggcgtcgc cactgacacg ccgctggcca tgatgttcac cggccagggt tcgcagcgcg
     2221 ccgggatggg ccgcgacttg tacgcccggt atccggtgtt cgccggggcc ttcgacgcgg
     2281 tgaccgcgca tctggacatc gactgggagg acctggacgc caccggtaac gcccagccgg
     2341 ccatcttcgc cgtcgaggtc gccctctacc ggctcctgga atcgtggggt gtcgtcccgg
     2401 acgtgttgct cggccactcg gtgggtgaga tcgccgccgc gcatctggcc ggggtcctct
     2461 ccctggagga cgcctgcacg ctgatctcgg cgcgcgggcg gctgatgcag gagttgccgg
     2521 ccggtggggc gatggtcgcg gtggaggcct cggaagggga cgtcagagcg gctctggtcg
     2581 atggtgtgga catcgcggcg gtcaacggtc cggacgcggt cgtgctgtcc ggtgcggcgg
     2641 ccgcggtgga ggcggtggcc gccggcttta ggcggagcaa gcggctgacc gtcagccatg
     2701 ccttccattc ggcactgatg gacccgatgt tggaggcctt ccggcaggtg gtcgacggcc
     2761 tcaccctgca ccggccgcag atcccgctgg tctccaacgt caccgggcgg atcgagaccg
     2821 acctgttcac cgacccggcg tactgggtgc ggcacgtgcg tgagacggtg cggttcgcgg
     2881 acgcggtggg tgccgcgggt gacgtgtggt tgctggaggt gggcccggac gcggtgctgt
     2941 ccgccatggc cggcggcagc cccaccctgc ggcgggaccg cgacgaggtc accaccctgc
     3001 tgaccgccgt gggtgcgttg tacacccagg gccggagtgt cgactgggcc gccgtcgtgg
     3061 acagcggacg ccgtatcgac ctgcccacgt acccgttcca gcaccagcga ttctggcccg
     3121 ccagcatgat cctcggcggt gccgctccgg gcctcgacgc agccgggcac ccgctgctca
     3181 gcggcgtgac caccctgccc ggctcggacg cgttgctgtt caccggccgc ctctccacct
     3241 cggcgcagcc gtggctcgcc gaccacgaga tgcagggcag ggtcatggtg cccggcaccg
     3301 ccctggtcga gctggccctg cacgccggcg ccgaggccgg cacgcccggc ctggaccagc
     3361 tcacccttac cgctccactg gtgctgcccg aacacggcgg caccctgcag gtgcaggtat
     3421 gggccggtgc cccggacggc tccggccgcc gcgcggtgac ggtgcactcg cgcccggacg
     3481 gcgaccccga cgcggtctgg acggaacacg ccaccgggct gctcggcatg gcccccaccc
     3541 cggtcacctt cgacgcggcc gcctggccac cgcccggcgc ccggccggtc ggcatcgacg
     3601 gactctacga ccggctcgcc gacggtggat tcggctacgg ccccgccttc cagggactgc
     3661 gcggcgcctg gcgcgacggc gagcacatgt acgccgaggt caccctcccc gccgatgccg
     3721 gcaccgagcg gtacggcctg cacccggccc tgttcgacac cgccctgcac gccctcctgc
     3781 tggacaccgc cgacagcccg gcgggcatcc cgttcgcctg gcacggcgtc gccctgcacg
     3841 ccgccggcgc gaccgacgcc cgcgtccggc tcacccggaa ccgggagggt ttcgccatcg
     3901 cgctggccga cccgaccggc gcgccgatcg cctcggccga ttcgctgctc gtccgcgccc
     3961 cccagccccg caccgccacc gactcgctgt accgcgtgga ctgggtaccg gtgacgctgc
     4021 cggctgccac cgccgcggcc accgtggtcg tcgtcgaggc aggcgaggag ccgcggacgg
     4081 cggtcctgga ggcgctcgcc cgggtgcagg agttcctggc cggcgcggat ccggagcagc
     4141 ggctggtctt cgtgacccgc cccggtgacc tggccggcgc ggcggtgtcc ggtctgatcc
     4201 gctcggccca gtccgagcac cccggccggt tcgggctgat cgagaccggc ggcgacgtac
     4261 cggacgacgc cctggccgcc gacgaaccgc ggctggccct gcacgacggc gtggccacgg
     4321 ttccgcgcct ggtgcgcgta ccggcggcgc ccgtggcggc ggatccgctg agcggcaccg
     4381 ttctgatcac cggcggcacc ggtggtctgg gcgcgctcat cgcccggcac ctggcggcgc
     4441 agggggtcac cgatctggtc ctggtgagcc gccgcggccc cgacgccccg ggcgccggcg
     4501 agctgcccgg ccgcgtggtg gcctgtgacg tcgccgatcg ctcggcggtg gacgccttgg
     4561 tggccgggct gcccgagctg tgcgcggtca tccatgccgc cggtgtcctc gacgacggtg
     4621 tcctcgcgtc gctcgatgcc gagcgggtcg acgcggtcct ggcacccaag atcacggcag
     4681 cctggaacct gcacgcggcc acccgggacc gtgacctcac cgcgttcgtg ctgttctcct
     4741 cgatcgccgg cctgttcggc aaccccggac aggcgagcta tgcggccggc aacgcctacc
     4801 tggacgcgct cgccgagacc cggcgcgcgg ccgggctgcc cggcacgtcg ctcgcctggg
     4861 ggccgtgggt ccggtccggc ggaatgaccg cggaactgac cgacggggac gtggcccgga
     4921 tgacccgcgc cggcctgccg ccgctcacgc aggagcaggg cctggccctg ttcgacgccg
     4981 cgctggcgat ccccgccgcg accctcgttc cggtccgcct cgacctcggc gcgctccgcg
     5041 ctcggggcga cgttccgccc ctgctgcgcg gcctggtgcg cgggccgtcg cgccgggcgg
     5101 ccgccgtggg tagcgccgcc ctggttcggc ggctcgccgc cctcgggccg caggaacgca
     5161 cggaggcggt gctcgacgtg gtgcgtaccc agatcgccca ggtgctcggc cacaccggca
     5221 ccgatgcggt ggagcccggc aaagccttcc aggatctcgg tttcgactcg ctcaccgcga
     5281 tcgagctgcg caaccgtctc ggcgccgcca ccggcctcgg tctgagcgcc accctggtgt
     5341 tcgactatcc gaacgccgcc gccctggccg cccacctgcg cgacgaactg ctcggcacgg
     5401 tcgccgagac gctcactcgc accccggtgg tgtcgccggt ctccgacgac ccggtggtca
     5461 tcgtgggcat ggcctgccgg taccccggcg gcgtcacctc tcccgaggat ctgtggcggc
     5521 tcgtgcacga cggggtggac gcggtcgggg acttcccgtc cgaccgcggc tgggacgtgg
     5581 acagcctcta cagcaccgac cggaacgcgc tgggcacctc gtacacccgg tggggcggct
     5641 tcctcgacga cgcggccggc ttcgacccgg agttcttcgg gatgagtccc cgggaggcgc
     5701 tggccaccga ctcgcagcag cggctgctcc tcgagtcggt gtgggaggcg gtggagcggg
     5761 ccggcatcga tccgcacacc ctgcgcggct cgcagaccgg cgtcttcgcc ggcgtcatgt
     5821 acaacgacta cgcgaacctg ctcggcggtg gccagtacga gggctaccag ggcagtggca
     5881 gcgccggcag catcgcctcg ggccgggtct cgtacacgtt cggcttcgag ggtccggcgg
     5941 tcaccgtgga cacggcctgt tcctcgtcgc tggtggcgtt gcacctgggg gcgcaggcgc
     6001 tgcgggcggg cgaatgtgat ctggcgttgg cgggcggcgt gacggtgatg tcgacgccca
     6061 gcaccttcgt ggagttctcc cggcagggcg gcctgtcggc ggacgggcgg tgcaagccgt
     6121 tcgcggacgc ggccgacggt gtcggctggg gcgagggcgt cggtgtgctg gtgctggagc
     6181 ggctctccga cgcccggcgt cacggccacc agatcctcgc ggtcgtccgc ggttcagcgg
     6241 tcaactccga cggcgcgtcg aacgggttga cggcgccgaa cgggccgtcg caacagcggg
     6301 tcatccggca ggccgtggcg aacgcgggac tgagcttccg ggacgtggac gtggtggagg
     6361 cgcacggcac gggtacgacg ctgggcgacc cgatcgaggc ccaggcgctg ctggccacgt
     6421 acggccggga ccgcgacgag ccgttgctgc tgggttcggt caagtcgaac atcgggcaca
     6481 cccaggccgc cgcgggcgtc gccggcgtga tcaagatggt cgtggccatg cgccacgggg
     6541 tcgtaccgcg caccctgcat gtggacgcgc cgtcgtcaca cgtggactgg gaggccggcg
     6601 cggtcgcgct ggcgcgggag aacaccccct ggccggactc cccgaagcgc cgggccggtg
     6661 tctcgtcgtt cggcatctcc ggcaccaacg cgcacgtcat cctggaacag ccggacccgc
     6721 ccgcggtgcc cgcggagcgg cccgccaccg acgaggtcgt gccgctggtc gtctccgcca
     6781 tgtcggcggg cgccctgcgc gaccaggtcg cccgtctcgc cgacgtcgcc ggcgacccgc
     6841 gcgacatcgg ctggtcgctt gccaccaccc ggtccgagtt cgcgcaccgg gcggtacggc
     6901 tcggtcccac cctgatcgag ggcgaggccc gtacccggcg cacggccttc atgttcaccg
     6961 gtcagggttc gcagcgggcg ggcatgggtc gtggcttgta tgagcggttt
//
