LOCUS       NZ_BMQY01000022         8527 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Couchioplanes caeruleus subsp. azureus strain JCM 3246 sequence22,
            whole genome shotgun sequence.
ACCESSION   NZ_BMQY01000022
VERSION     NZ_BMQY01000022.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Couchioplanes caeruleus subsp. azureus
  ORGANISM  Couchioplanes caeruleus subsp. azureus
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Couchioplanes.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 64X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,801
            CDSs (total)                      :: 6,731
            Genes (coding)                    :: 6,630
            CDSs (with protein)               :: 6,630
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 20 of 101
            Pseudo Genes (incomplete)         :: 93 of 101
            Pseudo Genes (internal stop)      :: 0 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:23:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8527
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMQY01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..3109
                     /locus_tag="IEY99_RS33595"
     source          1..8527
                     /culture_collection="JCM:3246"
                     /db_xref="taxon:56428"
                     /mol_type="genomic DNA"
                     /organism="Couchioplanes caeruleus subsp. azureus"
                     /strain="JCM 3246"
                     /sub_species="azureus"
                     /submitter_seqid="sequence22"
                     /type_material="type strain of Couchioplanes caeruleus
                     subsp. azureus"
     region          1..8527
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..8527
                     /SMILES="C=CC=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8527
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3145:>8527](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3146..>8527
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             <3..3109
                     /NRPS_PKS="Domain: PKS_AT (1-157). E-value: 1.1e-50. Score:
                     164.6. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33595_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (232-360). E-value: 2.1e-36.
                     Score: 117.8. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33595_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (612-775). E-value: 2.1e-55.
                     Score: 179.4. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33595_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (879-952). E-value: 1.5e-34. Score:
                     110.1. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33595_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (963-1031). E-value:
                     2.1e-19. Score: 62.1. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33595_PKS_Docking_Cterm.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012901.5"
                     /locus_tag="IEY99_RS33595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_189208164.1"
                     /sec_met_domain="PKS_AT (E-value: 3.7e-52, bitscore: 164.6,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.3e-55, bitscore:
                     175.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 9.2e-09, bitscore:
                     21.8, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.4e-17, bitscore:
                     50.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SARARLMQALPPGGAMIAVQGHESDVPEGVSVAAVNGPDSLVLSG
                     AEAAVEAVAAGFARSRRLAVSHAFHSALMDPMLAEFRAVVATLTLHEPTLPMVSTVTGS
                     RVEPGSVTDPDYWVRHVRETVRFADGVAAMRDLGVDTVVEVGPDAALSALIDADVVVPT
                     LRRGRDEVEAILAAAATLYVHGIDLDWTPWYPGAHRIALPTYAFQRAHYWPRPASSGGD
                     VTSLGQTAARHPLLGAAVAVAGADEVIFTGLLSLRVHPWLAEHDTVPGATWLELALQAG
                     DQVGCDRVARLEIGASLALTEQSAVALQVRVGAPGEDGGRAITVHSRTGDGPWIEHASG
                     RLAEDGGAPYEIGDTPVEAALPDGVADDARYFGLHPELLTALLDDDLEPAVFTGVTLHA
                     AGAGGLRATLTRRGTNGYRIAAVDPAGAPVLSVDSVRLREPVSVSGDQGPRGSLLDLRW
                     AAAEAAPAGRTVTLTGSLDDLRGDVDLVLVPIAGAGEDVVAEAHRLGAYALDLVQRWLA
                     TERFGDARLAFVTAGAVGTGDSDRVRDVAASVVWGLVRAAQSEHPDRFLLIDHEAGAPV
                     DALPSDEPQFAIREGRVLVPRLARTAEREPGGWTPRGTVLITGGTGGLGSALARHLAAR
                     GVDLVLVSRRGPDAPGADRLPGRVVACDLTDRAAVDELVAGIPDLCAVVHTAGVLDDGV
                     VTALTPERLDAVLAPKVDAAWNLHAATRDLDAFVLFSSISGVMGSAGQANYAAGNVFLD
                     ALAAYRQSRGLAAQSLAWPAWAGDVGMTASLGEKDMHRASSAMPPLTVEQGLALFDSAV
                     AAGSPYLVPVSMSSTKVRMPGPVPAVLRDLVKGGRARAAGNAGPAATLAALTQQLAGRR
                     EDERLRHVTDLVRAEAAAVLGHASAAAVDARREFRDLGFDSLTAVELRNRLTAVTGLRL
                     SATMVFDHPTPAALAEHLTKELVSEHTAQEGPALLADLDRLGAALRASEPDDVTRAAVT
                     ARLRRLLDAWRADEPGRADVAERLGAASTDDLFAFIDNELGRQSDR"
     aSDomain        8..475
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: False, scaffold matched
                     GHGE: False"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33595_PKS_AT.1"
                     /evalue="1.10E-50"
                     /label="IEY99_RS33595_PKS_AT.1"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="157"
                     /protein_start="1"
                     /score="164.6"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: (unknown)"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="ARARLMQALPPGGAMIAVQGHESDVPEGVSVAAVNGPDSLVLSGA
                     EAAVEAVAAGFARSRRLAVSHAFHSALMDPMLAEFRAVVATLTLHEPTLPMVSTVTGSR
                     VEPGSVTDPDYWVRHVRETVRFADGVAAMRDLGVDTVVEVGPDAALSALIDA"
     aSModule        8..2860
                     /complete
                     /domains="nrpspksdomains_IEY99_RS33595_PKS_AT.1"
                     /domains="nrpspksdomains_IEY99_RS33595_PKS_DH.1"
                     /domains="nrpspksdomains_IEY99_RS33595_PKS_KR.1"
                     /domains="nrpspksdomains_IEY99_RS33595_ACP.1"
                     /locus_tags="IEY99_RS33595"
                     /monomer_pairings="mal -> ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       101..139
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33595_0001"
                     /evalue="8.40E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="45"
                     /protein_start="32"
                     /score="12.6"
                     /tool="antismash"
                     /translation="AAVNGPDSLVLSG"
     CDS_motif       350..385
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33595_0002"
                     /evalue="2.80E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="127"
                     /protein_start="115"
                     /score="17.3"
                     /tool="antismash"
                     /translation="YWVRHVRETVRF"
     aSDomain        701..1084
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33595_PKS_DH.1"
                     /evalue="2.10E-36"
                     /label="IEY99_RS33595_PKS_DH.1"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="360"
                     /protein_start="232"
                     /score="117.8"
                     /tool="antismash"
                     /translation="HPLLGAAVAVAGADEVIFTGLLSLRVHPWLAEHDTVPGATWLELA
                     LQAGDQVGCDRVARLEIGASLALTEQSAVALQVRVGAPGEDGGRAITVHSRTGDGPWIE
                     HASGRLAEDGGAPYEIGDTPVEAA"
     aSDomain        1841..2329
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33595_PKS_KR.1"
                     /evalue="2.10E-55"
                     /label="IEY99_RS33595_PKS_KR.1"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="775"
                     /protein_start="612"
                     /score="179.4"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLITGGTGGLGSALARHLAARGVDLVLVSRRGPDAPGADRLPG
                     RVVACDLTDRAAVDELVAGIPDLCAVVHTAGVLDDGVVTALTPERLDAVLAPKVDAAWN
                     LHAATRDLDAFVLFSSISGVMGSAGQANYAAGNVFLDALAAYRQSRGLAAQSLAWPAWA
                     "
     CDS_motif       1844..1939
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33595_0003"
                     /evalue="1.30E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="645"
                     /protein_start="613"
                     /score="33.8"
                     /tool="antismash"
                     /translation="TVLITGGTGGLGSALARHLAARGVDLVLVSRR"
     CDS_motif       2129..2158
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33595_0004"
                     /evalue="2.10E-03"
                     /label="PKSI-KR_m3"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="718"
                     /protein_start="708"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PKVDAAWNLH"
     CDS_motif       2174..2284
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33595_0005"
                     /evalue="4.60E-21"
                     /label="PKSI-KR_m4"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="760"
                     /protein_start="723"
                     /score="67.4"
                     /tool="antismash"
                     /translation="LDAFVLFSSISGVMGSAGQANYAAGNVFLDALAAYRQ"
     aSDomain        2642..2860
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33595_ACP.1"
                     /evalue="1.50E-34"
                     /label="IEY99_RS33595_ACP.1"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="952"
                     /protein_start="879"
                     /score="110.1"
                     /tool="antismash"
                     /translation="TDLVRAEAAAVLGHASAAAVDARREFRDLGFDSLTAVELRNRLTA
                     VTGLRLSATMVFDHPTPAALAEHLTKEL"
     aSDomain        2894..3097
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33595_PKS_Docking_Cterm.
                     1"
                     /evalue="2.10E-19"
                     /label="IEY99_RS33595_PKS_Docking_Cterm.1"
                     /locus_tag="IEY99_RS33595"
                     /protein_end="1031"
                     /protein_start="963"
                     /score="62.1"
                     /tool="antismash"
                     /translation="LLADLDRLGAALRASEPDDVTRAAVTARLRRLLDAWRADEPGRAD
                     VAERLGAASTDDLFAFIDNELGR"
     gene            3146..>8527
                     /locus_tag="IEY99_RS33600"
     CDS             3146..>8527
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (4-30). E-value:
                     8.4e-17. Score: 52.4. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33600_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-453). E-value:
                     3e-177. Score: 581.7. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33600_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (536-794). E-value: 5.4e-90.
                     Score: 293.7. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33600_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (867-994). E-value: 6.9e-33.
                     Score: 106.3. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33600_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1248-1424). E-value: 6.6e-60.
                     Score: 194.1. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33600_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1528-1601). E-value: 8.5e-31.
                     Score: 98.1. Matches aSDomain:
                     nrpspksdomains_IEY99_RS33600_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013477280.1"
                     /locus_tag="IEY99_RS33600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189208165.1"
                     /sec_met_domain="mod_KS (E-value: 1.3e-219, bitscore:
                     716.3, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.1e-90, bitscore:
                     288.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.8e-91, bitscore: 293.7,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1e-62, bitscore:
                     198.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 8.6e-15, bitscore:
                     42.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDDSKLIDYLKWVTADLHETRRRLQEVESGRQEPIAIVGMACRL
                     PGGVRGAEALWQLVDEGRDGVTAFPDDRGWDAAALGGDGEGSSATGEGGFIAAGDFDAG
                     FFGISPREAVAMDPQQRLLLETSWEAFEHAGINPASLRGTRTGVFTGASGVDYIGVVMG
                     SREDAEGHATTGLTSSVISGRVAYTLGLEGPALTVDTACSSSLVALHLAAQALRSGECE
                     LALAGGVTVMSTPMGFSGFTRQGGLAPDGRCKAFADAADGTGWSEGVGVLVVEKLSDAR
                     RNNHRILAVVRGSAVNSDGASNGLTAPNGPSQQRVIRQALAGAGLTPRDVDVVEAHGTG
                     TTLGDPIEAQALLATYGQDRSEPLYLGSVKSNIGHTQAAAGVAGVIKMVMAMRHGTVPR
                     TLHVDAPTSHVDWSQGAVELLTEPRPWPAADRPARAGVSSFGISGTNAHVIIEQGETPA
                     YEPSPAPAAAPWPVSARSETALDAQIARLRAWVAQRPELSAADVGRSLAVTRAQLAHRA
                     VLIGDTVVRGVATRRRLGVVFSGQGSQRVGMGRALYQRFPAYRAAFDEVAAHLAIDWDD
                     LDSTGNAQPAIFAVEVALYRLLESWGVRADAVAGHSVGEITAAHVAGVLSLPDACALIS
                     ARARLMQALPAGGAMIAVQAAESDLSLGADVSIAAVNGPDSVVLSGDEAAVTAAAAQFA
                     RTKRLTVSHAFHSALMDPMLDAFADAIAGITLDPPSCDLVSNVTGRIESDLFTDPAYWV
                     RHVRETVRFADGLAAMSGSGVDAFLEVGPDSVLSALIDAELVVPTLRRDRDENESMLAA
                     AAALHVGGVDVDFAAWYPDATVVPLPTYAFEHQRFWPRPAAGHGAVSAAGLIAAQHPLL
                     GAAVAVADADEVILTGLLSPRAHPWLAGRATMPGAAWLELAIRAGDQVGCDHVRLLDIG
                     AALAVDERPVAIQVRVHAPGPDGDRRITMHARADDAPWVQHAEGVLSAAAAEPPADTDG
                     AGAEVSLGDADAADARYFGLHPALLTMLLDEQSEPASFAGVTLHAAGARTLHARLRRSG
                     DTVRVVAVDPADAPVLTIDALTLREPLAVAGGRTRPGSLLTLDWVAPRAAERRPGLLVV
                     EGTDDVPAGADLVLIRIRTGDDVVADAHRHAATALESMQRWLADSHGAPLVFVTSGAVT
                     GADPAAAAVWGLVRSAQTENPGRFALLDAGSAGEADAVLPLLPGLIATGDGQFVVRDGE
                     LTVGRLDRVPADVHAGPSPWTGGGTVLITGGTGSLGSALARHLVVDHGVARLVLVSRRG
                     PDAPGAAELTRELRAAGAEVLVAACDLSDARAVRALVDAEPDLSAVVHTAGVLDDGILT
                     SLTPQRLSAVLAPKVDGAWNLHLATASRRLDAFVLFSSISGVMGAPGQANYAAGNVFLD
                     ALARHRRAAGLPAQSLAWAAWAPGAGMTSTLSDADLQRVSSGMPPLTPEQGLALFDAAV
                     TVDEPYLVPVSMSSGNLRMPGEVPAVLRALIRTGRRPAATTAGGAATFAGISRELLSRP
                     AAQRLAYVLDLVRAEVAAVLAHACAEAVDAQREFRDLGFDSLTAVELRNKLAAATGLRL
                     SPTMVFDYPTPTVLAGFLLGELLGEEDDAEMPATVTADVTADPIVIVGMACRLPGGVTS
                     PDELWDLVAGGRDGISPFPADRGWDLTELRQGGEGGRGRSATDRGGFLERVADFDAGFF
                     GISPREALAMDPQQRLLLEASWEAFERAGIAPGGLRGSRTGVFVGCGGQDYTQVVMNSR
                     EDIEGHAQTGLAASVVSGRVSYTLGLEGPAVTVDTACS"
     aSDomain        3158..3235
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33600_PKS_Docking_Nterm.
                     1"
                     /evalue="8.40E-17"
                     /label="IEY99_RS33600_PKS_Docking_Nterm.1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="30"
                     /protein_start="4"
                     /score="52.4"
                     /tool="antismash"
                     /translation="SKLIDYLKWVTADLHETRRRLQEVES"
     aSDomain        3251..4504
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33600_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="3.00E-177"
                     /label="IEY99_RS33600_PKS_KS.1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="453"
                     /protein_start="35"
                     /score="581.7"
                     /tool="antismash"
                     /translation="IAIVGMACRLPGGVRGAEALWQLVDEGRDGVTAFPDDRGWDAAAL
                     GGDGEGSSATGEGGFIAAGDFDAGFFGISPREAVAMDPQQRLLLETSWEAFEHAGINPA
                     SLRGTRTGVFTGASGVDYIGVVMGSREDAEGHATTGLTSSVISGRVAYTLGLEGPALTV
                     DTACSSSLVALHLAAQALRSGECELALAGGVTVMSTPMGFSGFTRQGGLAPDGRCKAFA
                     DAADGTGWSEGVGVLVVEKLSDARRNNHRILAVVRGSAVNSDGASNGLTAPNGPSQQRV
                     IRQALAGAGLTPRDVDVVEAHGTGTTLGDPIEAQALLATYGQDRSEPLYLGSVKSNIGH
                     TQAAAGVAGVIKMVMAMRHGTVPRTLHVDAPTSHVDWSQGAVELLTEPRPWPAADRPAR
                     AGVSSFGISGTNAHVIIEQ"
     aSModule        3251..7948
                     /complete
                     /domains="nrpspksdomains_IEY99_RS33600_PKS_KS.1"
                     /domains="nrpspksdomains_IEY99_RS33600_PKS_AT.1"
                     /domains="nrpspksdomains_IEY99_RS33600_PKS_DH.1"
                     /domains="nrpspksdomains_IEY99_RS33600_PKS_KR.1"
                     /domains="nrpspksdomains_IEY99_RS33600_ACP.1"
                     /locus_tags="IEY99_RS33600"
                     /monomer_pairings="mal -> ccmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       3494..3520
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0001"
                     /evalue="2.90E-02"
                     /label="PKSI-KS_m2"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="125"
                     /protein_start="116"
                     /score="7.6"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       3722..3769
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0002"
                     /evalue="9.40E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="208"
                     /protein_start="192"
                     /score="20.7"
                     /tool="antismash"
                     /translation="GPALTVDTACSSSLVA"
     CDS_motif       4145..4189
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0003"
                     /evalue="3.80E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="348"
                     /protein_start="333"
                     /score="12.7"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       4244..4288
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0004"
                     /evalue="6.80E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="381"
                     /protein_start="366"
                     /score="12.1"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     aSDomain        4754..5527
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33600_PKS_AT.1"
                     /evalue="5.40E-90"
                     /label="IEY99_RS33600_PKS_AT.1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="794"
                     /protein_start="536"
                     /score="293.7"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="VFSGQGSQRVGMGRALYQRFPAYRAAFDEVAAHLAIDWDDLDSTG
                     NAQPAIFAVEVALYRLLESWGVRADAVAGHSVGEITAAHVAGVLSLPDACALISARARL
                     MQALPAGGAMIAVQAAESDLSLGADVSIAAVNGPDSVVLSGDEAAVTAAAAQFARTKRL
                     TVSHAFHSALMDPMLDAFADAIAGITLDPPSCDLVSNVTGRIESDLFTDPAYWVRHVRE
                     TVRFADGLAAMSGSGVDAFLEVGPDSVLSALIDAEL"
     CDS_motif       4757..4804
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0005"
                     /evalue="9.80E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="553"
                     /protein_start="537"
                     /score="18.1"
                     /tool="antismash"
                     /translation="FSGQGSQRVGMGRALY"
     CDS_motif       4889..4939
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0006"
                     /evalue="3.30E-03"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="598"
                     /protein_start="581"
                     /score="10.8"
                     /tool="antismash"
                     /translation="NAQPAIFAVEVALYRLL"
     CDS_motif       4949..5071
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0007"
                     /evalue="1.00E-19"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="642"
                     /protein_start="601"
                     /score="62.7"
                     /tool="antismash"
                     /translation="GVRADAVAGHSVGEITAAHVAGVLSLPDACALISARARLMQ"
     CDS_motif       5150..5188
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0008"
                     /evalue="3.00E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="681"
                     /protein_start="668"
                     /score="14.0"
                     /tool="antismash"
                     /translation="AAVNGPDSVVLSG"
     CDS_motif       5396..5431
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0009"
                     /evalue="5.30E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="762"
                     /protein_start="750"
                     /score="16.5"
                     /tool="antismash"
                     /translation="YWVRHVRETVRF"
     aSDomain        5747..6127
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33600_PKS_DH.1"
                     /evalue="6.90E-33"
                     /label="IEY99_RS33600_PKS_DH.1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="994"
                     /protein_start="867"
                     /score="106.3"
                     /tool="antismash"
                     /translation="HPLLGAAVAVADADEVILTGLLSPRAHPWLAGRATMPGAAWLELA
                     IRAGDQVGCDHVRLLDIGAALAVDERPVAIQVRVHAPGPDGDRRITMHARADDAPWVQH
                     AEGVLSAAAAEPPADTDGAGAEV"
     aSDomain        6890..7417
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33600_PKS_KR.1"
                     /evalue="6.60E-60"
                     /label="IEY99_RS33600_PKS_KR.1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1424"
                     /protein_start="1248"
                     /score="194.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLITGGTGSLGSALARHLVVDHGVARLVLVSRRGPDAPGAAEL
                     TRELRAAGAEVLVAACDLSDARAVRALVDAEPDLSAVVHTAGVLDDGILTSLTPQRLSA
                     VLAPKVDGAWNLHLATASRRLDAFVLFSSISGVMGAPGQANYAAGNVFLDALARHRRAA
                     GLPAQSLAWAAWA"
     CDS_motif       6893..6994
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0010"
                     /evalue="4.10E-10"
                     /label="PKSI-KR_m1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1283"
                     /protein_start="1249"
                     /score="32.3"
                     /tool="antismash"
                     /translation="TVLITGGTGSLGSALARHLVVDHGVARLVLVSRR"
     CDS_motif       7211..7240
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0011"
                     /evalue="3.70E-04"
                     /label="PKSI-KR_m3"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1365"
                     /protein_start="1355"
                     /score="13.7"
                     /tool="antismash"
                     /translation="PKVDGAWNLH"
     CDS_motif       7262..7372
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0012"
                     /evalue="8.40E-23"
                     /label="PKSI-KR_m4"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1409"
                     /protein_start="1372"
                     /score="73.0"
                     /tool="antismash"
                     /translation="LDAFVLFSSISGVMGAPGQANYAAGNVFLDALARHRR"
     aSDomain        7730..7948
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS33600_ACP.1"
                     /evalue="8.50E-31"
                     /label="IEY99_RS33600_ACP.1"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1601"
                     /protein_start="1528"
                     /score="98.1"
                     /tool="antismash"
                     /translation="LDLVRAEVAAVLAHACAEAVDAQREFRDLGFDSLTAVELRNKLAA
                     ATGLRLSPTMVFDYPTPTVLAGFLLGEL"
     CDS_motif       8267..8293
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0013"
                     /evalue="2.90E-02"
                     /label="PKSI-KS_m2"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1716"
                     /protein_start="1707"
                     /score="7.6"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       8495..>8527
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS33600_0014"
                     /evalue="6.30E-03"
                     /label="PKSI-KS_m3"
                     /locus_tag="IEY99_RS33600"
                     /protein_end="1794"
                     /protein_start="1783"
                     /score="9.4"
                     /tool="antismash"
                     /translation="GPAVTVDTACS"
ORIGIN
        1 gtcggcgcgg gcccggttga tgcaggcgtt gccgcccggc ggcgcgatga tcgcggtgca
       61 gggtcacgag agcgatgtgc ccgaaggggt gtcggtcgcc gccgtcaacg ggccggactc
      121 gctggtgttg tccggtgcgg aagccgcggt ggaagccgtc gcggcgggct tcgcgcgcag
      181 cagacgcctc gctgtcagtc atgccttcca ctccgcgttg atggacccga tgctggccga
      241 gttccgggcg gtcgtggcga cgctgacgct gcacgagccg accctgccga tggtctccac
      301 cgtcaccggc tcgcgggtcg aacccggttc ggtgacggac ccggactact gggtgcggca
      361 cgtgcgcgag acggtccggt tcgcggacgg ggttgccgcc atgcgcgacc tcggcgtgga
      421 caccgtcgtc gaggtcgggc ccgacgcggc actgtccgcg ctgatcgacg ccgacgtcgt
      481 ggtgccgacc ctgcgccgcg gccgggacga ggtggaggcg atcctcgccg cggccgccac
      541 cctgtacgtg cacggcatcg acctggactg gaccccctgg tatccgggtg cgcaccgcat
      601 cgccctgccc acgtacgcgt tccagcgcgc gcactactgg ccgcgcccgg cctcctccgg
      661 gggcgacgtc acctcgctcg ggcagaccgc cgcccgccat ccgctgctcg gcgccgcggt
      721 cgcggtggcc ggcgccgacg aggtcatctt cacgggactg ctgtcgctgc gcgtccaccc
      781 gtggctcgcc gagcacgaca ccgtgcccgg cgccacctgg ctggaactgg ccctgcaggc
      841 cggcgaccag gtcggctgcg accgggtggc acgcctggag atcggtgcgt ccctcgcact
      901 caccgagcag tcggcggtgg ccctgcaggt acgggtcggt gcgcccggcg aggacggcgg
      961 ccgcgcgatc acggtccact cgcgtaccgg cgacgggccg tggatcgagc atgcctccgg
     1021 ccggctcgcc gaggacggcg gtgcgccgta cgagatcggc gacacgccgg tggaggcggc
     1081 cctgccggac ggtgtcgccg acgatgcgcg ctacttcggt ctgcacccgg aactgctcac
     1141 cgccctgctc gacgacgacc tcgaaccggc cgtgttcacc ggcgtcaccc tccacgcggc
     1201 cggggccggc ggcctgcgcg ccacgctgac ccggcggggt acgaacggtt accggatcgc
     1261 cgccgtcgac ccggccggtg caccggtgct gtccgtcgac tcggtgcgac tacgcgaacc
     1321 ggtatccgtc tccggtgacc aggggccgcg cggatcgctg ctggacctgc gctgggccgc
     1381 cgccgaggcg gcccccgcgg gccgcacggt caccctcacc ggctccctgg acgacctgcg
     1441 cggtgacgtc gatctggtgc tcgtgccgat cgccggcgcc ggcgaggacg tggtggccga
     1501 ggcgcaccgg ctcggcgcgt acgccctcga tctggtccag cggtggttgg ccaccgagcg
     1561 gttcggcgac gcgcggctcg cgttcgtcac cgccggcgcg gtcggcaccg gcgacagtga
     1621 ccgggtccgc gacgtcgcgg cgagcgtcgt ctggggtctg gtccgggccg cccagtccga
     1681 gcacccggac cggttcctgt tgatcgacca cgaggccggc gcccccgtgg acgcgctgcc
     1741 ctccgacgag ccgcagttcg ccatccgcga gggtcgggtg ctggtgccgc ggctggcccg
     1801 tacggccgag cgggagcccg gtgggtggac gccgcggggc acggtgttga tcaccggtgg
     1861 taccggtggt ctgggttcgg cgctggcgcg tcaccttgct gctcgtggtg tggatctggt
     1921 gctggtcagc cgccgtggtc cggacgcgcc gggcgcggac cggttgccgg gccgggtggt
     1981 ggcctgtgat ctgacggatc gtgcggcggt ggatgagctc gtcgcaggca ttccggatct
     2041 gtgcgcggtg gtccataccg cgggtgtgct cgacgacggg gtggtgaccg cgctgacccc
     2101 cgagcggctc gacgcggtgc tcgctcccaa ggtcgacgcg gcgtggaatc tgcacgcggc
     2161 gacgcgggac ctggatgcct tcgtgctgtt ctcctcgatc tcgggggtga tgggcagcgc
     2221 cgggcaggcg aactatgcgg ccggcaacgt gttcctggat gcactggcgg cgtaccggca
     2281 gtcgcggggg ctcgccgcgc agtcgctggc ctggccggcc tgggccggtg acgtcggtat
     2341 gaccgcgtca ctcggtgaga aggacatgca ccgcgcctcc tccgccatgc cgccgctcac
     2401 cgtggagcag gggctcgcgc tcttcgacag cgccgtggcg gcggggtcgc cgtacctcgt
     2461 gccggtcagc atgtcgtcca ccaaggtccg gatgcccggt ccggtcccgg ccgtcctgcg
     2521 cgacctggtc aagggcgggc gcgcccgggc ggccgggaac gccggaccgg cagcgaccct
     2581 ggccgcgctc acccagcaac tcgccggacg ccgcgaggac gagcgcctgc gccacgtcac
     2641 cgacctcgtc cgtgccgagg ccgccgcggt gctcggccac gcctcggccg ccgccgtcga
     2701 cgcgcgccgt gagttccgtg acctcggctt cgactcgctc accgcggtgg agctgcgcaa
     2761 ccggctgacc gccgtcaccg gcctgcggct cagcgccacc atggtcttcg accacccgac
     2821 ccccgccgcg ctcgccgagc acctcaccaa ggagctggtc agcgagcaca ccgcgcagga
     2881 gggccccgcc ctgctcgcgg atctggaccg gctcggcgcc gccctgcggg ccagcgagcc
     2941 cgacgacgtc acccgggccg cggtcaccgc ccggctccgc cggcttctcg acgcgtggcg
     3001 cgccgacgag cccggccgcg ccgacgtggc cgaacggctc ggcgccgcgt ccaccgacga
     3061 tctgttcgcc ttcatcgaca acgaactcgg ccgccagagc gaccgctgaa ctcgcgcact
     3121 ccggtacgca cggaaggttg ctcgcatgtc cgacgacagc aagctcatcg actacctcaa
     3181 gtgggtcacg gccgacctgc acgagacccg ccgccgcctg caagaggtgg agtcgggccg
     3241 gcaggagccg atcgcgatcg tcggcatggc ctgccgcctc cccggcgggg tgcgcggggc
     3301 ggaagcgctg tggcagctgg tcgacgaggg acgcgacggc gtcaccgcct tccccgacga
     3361 ccgcggctgg gatgcggcgg cactcggcgg cgacggtgag ggcagcagcg ccaccgggga
     3421 gggcggcttc atcgcggcgg gcgacttcga cgccggcttc ttcggcatct cgccgcgcga
     3481 ggcggtcgcc atggaccccc agcagcgact gctactggag acctcgtggg aggcattcga
     3541 acacgccggg atcaacccgg cgagcctgcg tggtacccgc accggggtct tcaccggcgc
     3601 gtccggggtg gactacatcg gtgtcgtcat ggggtcgcgg gaagacgccg agggacatgc
     3661 caccaccggt ctgacctcca gcgtgatctc cggtcgggtg gcgtacacgc tcgggctcga
     3721 gggccccgcc ctgacggtgg acaccgcctg ctcgtcgtcg ctggtcgcat tgcacctggc
     3781 cgcgcaggcg ctgcgcagcg gcgagtgcga gctggcgctg gcgggcggcg tgacggtgat
     3841 gtccacaccg atgggcttct cgggcttcac ccggcagggc gggctcgctc cggacggtcg
     3901 ctgcaaggcg ttcgcggacg cggcggacgg cacgggctgg tccgaaggcg tcggcgtgct
     3961 ggtggtcgag aagctctccg acgcccggcg caacaaccac cgcatcctcg cggtggtccg
     4021 gggctcggcg gtcaactccg acggcgcctc gaacggcctg accgcgccga acgggccctc
     4081 gcagcagcgg gtcatccggc aggccctcgc cggcgccggc ctgaccccgc gcgacgtgga
     4141 cgtggtggag gcgcacggca ccggcacgac gctcggtgac ccgatcgagg cgcaggcgtt
     4201 gctcgccacg tacgggcagg atcgcagcga gccgctctac ctcggctcgg tgaagtcgaa
     4261 catcgggcac acgcaggccg cggccggggt ggccggtgtc atcaagatgg tcatggcgat
     4321 gcgacacggc acggtgccgc gcaccctgca cgtggatgcg cccacgtccc acgtggactg
     4381 gtcccagggt gcggtcgagc tgctcaccga gccccggccg tggcctgccg cggaccgccc
     4441 ggcccgcgcc ggagtgtcgt cgttcggcat ctccggcacc aacgcccacg tcatcatcga
     4501 gcagggcgag acgcccgcct acgagccgtc cccggccccc gcggccgcgc cgtggccggt
     4561 gtcggcgcgc agcgaaaccg ctctggacgc ccagatcgcc cggttgcggg cctgggtcgc
     4621 ccagcgcccc gagctgtccg cggccgacgt cggacgctcg ctcgccgtca cccgcgccca
     4681 gctggcgcac cgcgcggtac tcatcggcga caccgtcgtc cgcggtgtcg ccacgcgccg
     4741 ccgcctcggc gtggtcttct ccgggcaggg ctcgcagcgg gtcggaatgg gacgcgcgct
     4801 gtaccagcgg ttcccggcgt accgcgcggc cttcgacgag gtggcggcgc atctcgcgat
     4861 cgactgggac gacctggact ccaccgggaa cgcgcagccg gccatcttcg ccgtcgaggt
     4921 cgccctttac cgcctgctcg agtcgtgggg ggtgcgggcg gatgcggtgg ccggccactc
     4981 cgtcggcgag atcacggccg cgcatgtagc cggagtcctg tccctgccgg acgcctgcgc
     5041 gctgatcagt gcgcgggcga gactcatgca ggcattgccg gccggtggcg cgatgatcgc
     5101 cgtacaggcg gccgagtcgg acctctcgct cggtgcggac gtgtcgatcg cggcggtcaa
     5161 cggcccggac tccgtcgtcc tgtcgggcga cgaggctgcc gtcacggccg ccgccgcgca
     5221 gttcgcccgg accaagcggc tcaccgtcag ccacgccttc cactcggcgc tgatggatcc
     5281 gatgctggac gcgttcgccg acgccatcgc ggggatcacc ctcgacccgc cctcctgtga
     5341 tctggtctcc aacgtcaccg ggcgcatcga gtcggacctg ttcaccgacc ccgcctactg
     5401 ggtccggcac gtgcgcgaga ccgtccgctt cgccgacggc ctcgccgcga tgtccggatc
     5461 gggcgtggac gccttcctgg aagtcggccc ggactcggtg ctcagcgcgc tgatcgacgc
     5521 cgagctggtc gtgccgacgc tgcgccggga ccgcgacgag aacgagtcga tgctcgccgc
     5581 cgcggccgcc ctgcacgtcg gcggcgtcga cgtcgacttc gccgcctggt acccggatgc
     5641 gaccgtcgtg ccgctgccga cctacgcctt cgagcatcaa cgtttctggc cgcgcccggc
     5701 cgccggtcac ggcgccgtct ccgcggccgg cctgatcgcc gcccagcacc cgctgctcgg
     5761 ggcggccgtc gccgtggccg acgccgacga ggtcatcctc accggcctgc tcagcccgcg
     5821 cgcccacccc tggctcgcgg ggcgcgcgac catgcccggc gccgcctggc tggaactcgc
     5881 catccgggcc ggcgaccagg tcggctgcga ccacgtgcgc ctgctcgaca tcggtgccgc
     5941 cctggccgtc gacgagcggc cggtggcgat ccaggtccgg gtgcacgcgc ccggcccgga
     6001 cggcgaccgg cgcatcacca tgcatgcccg cgccgacgac gctccctggg tgcagcacgc
     6061 cgagggtgtg ctgtccgcag ccgccgccga accaccggcg gacaccgacg gcgcgggtgc
     6121 ggaggtgtcg ttgggtgacg ccgacgccgc cgacgcccgt tacttcggtc tgcatccggc
     6181 gctgctcacc atgctcctcg acgaacagtc ggaacccgcc tcgttcgccg gagtcacgct
     6241 gcacgcggcc ggcgcccgga ccctgcatgc ccgcctgcgg cgcagtggtg acaccgtgcg
     6301 cgtcgtcgcc gtcgacccgg ccgacgcgcc cgtgctcacc atcgacgccc tcaccctgcg
     6361 agagccgctc gcggtggccg gcgggcgtac ccgccccggc tcgctgctca ccctggactg
     6421 ggtcgcgccg cgcgccgccg aacgccgtcc cggtctcctc gtggtcgagg ggaccgatga
     6481 cgtcccggcc ggcgccgacc tggtcctgat ccgcatccgt accggggacg acgtggtcgc
     6541 ggacgcccac cggcacgccg ccacggcgct ggagtccatg cagcgctggc tggcggacag
     6601 ccacggcgcc ccgctggtct tcgtcacctc cggcgcggtc accggcgccg acccggccgc
     6661 cgcggcggtc tggggcctgg tccgctccgc ccagaccgag aacccgggcc ggttcgcgct
     6721 gctcgacgcc ggctccgctg gcgaggcgga cgcggtcctg ccgctgttgc ccggcctcat
     6781 cgccacgggc gacggccagt tcgtggtccg cgacggcgag ctcaccgtgg gccgcctcga
     6841 ccgggtcccg gccgacgtcc acgccggtcc ctcgccgtgg accggcggcg gcacggtgct
     6901 gatcaccggc ggcaccggca gtctcggctc ggccctggcc cgccacctgg tcgtcgacca
     6961 cggggtggcc cggctcgtgc tggtcagccg caggggcccg gacgcgccgg gtgccgccga
     7021 gctgacccgg gagctgcgcg ccgcgggcgc cgaggtgctg gtcgccgcct gcgacctgtc
     7081 cgacgcccgt gccgtccggg cgctggtgga tgccgagccg gacctgtccg cggtggtaca
     7141 caccgccggg gtgctggacg acgggatcct cacctcgctc accccgcagc ggctgtccgc
     7201 cgtcctggcg cccaaggtgg acggtgcgtg gaacctgcat ctcgccaccg cgtcccgccg
     7261 cctcgacgcg ttcgtgctgt tctcgtccat ctccggtgtc atgggcgcgc ccggacaggc
     7321 caactacgcg gcgggcaacg tcttcctcga cgccctcgcc cggcaccgca gagccgccgg
     7381 gctgccggcg cagtcgctcg cctgggccgc ctgggcgccc ggtgccggca tgacctccac
     7441 gctgtcggac gccgacctgc aacgcgtctc ctccgggatg ccgccgctca ccccggagca
     7501 gggtctcgcc ctcttcgacg cggccgtcac cgtcgacgag ccgtacctgg tgccggtcag
     7561 catgtcgtcc ggcaacctgc gcatgcccgg cgaggtgccg gcggtgctgc gtgccctgat
     7621 ccgtacggga cgccggccgg ctgcgacgac cgccggcggt gcggccacct tcgccggcat
     7681 ctcccgtgag ctgctgtcgc ggcccgccgc ccagcgcctc gcgtacgtgc tcgacctggt
     7741 ccgcgccgag gtcgccgccg tgctcgcgca cgcgtgcgct gaagccgtcg atgcgcagcg
     7801 cgagttccgt gacctcggct tcgactcgct caccgcggtg gagctgcgga acaagctcgc
     7861 cgcggcgacc ggcctgcggt tgagcccgac catggtgttc gactatccga cccccaccgt
     7921 gctggcgggg ttcctactcg gcgaactcct cggcgaggag gacgacgcgg agatgccggc
     7981 cacggtgacc gcggacgtca ccgccgaccc gatcgtcatc gtcggcatgg cgtgccgcct
     8041 gcccggcggt gtcacctcgc cggacgagct gtgggacctg gtcgccggcg gccgggacgg
     8101 catctcgccc ttccccgccg accgcggctg ggacctcacc gagctgcggc agggtggcga
     8161 gggcggccgc ggacgcagcg ccaccgatcg gggtggtttc ctggagcggg tggccgactt
     8221 cgacgccggc ttcttcggca tctcgccgcg cgaggcgctc gccatggacc cccagcagcg
     8281 actgctgctc gaagcctcct gggaggcgtt cgagcgggcc gggatcgccc cgggcgggct
     8341 gcgcggcagc cggaccggtg tcttcgtcgg ctgcggtgga caggactaca cccaggtggt
     8401 gatgaactcc cgggaggaca tcgagggcca cgcccagacc gggctcgcgg ccagcgtggt
     8461 ttccggccgg gtctcgtaca cgttgggtct ggagggtccg gcggtcaccg tggacacggc
     8521 gtgttcg
//
