LOCUS       NZ_BMQY01000007        10423 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Couchioplanes caeruleus subsp. azureus strain JCM 3246 sequence07,
            whole genome shotgun sequence.
ACCESSION   NZ_BMQY01000007
VERSION     NZ_BMQY01000007.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Couchioplanes caeruleus subsp. azureus
  ORGANISM  Couchioplanes caeruleus subsp. azureus
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Couchioplanes.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 358185)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 64X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,801
            CDSs (total)                      :: 6,731
            Genes (coding)                    :: 6,630
            CDSs (with protein)               :: 6,630
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 20 of 101
            Pseudo Genes (incomplete)         :: 93 of 101
            Pseudo Genes (internal stop)      :: 0 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:23:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 303861
            Orig. end                         :: 314284
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMQY01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5423)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(311..1831)
                     /locus_tag="IEY99_RS24975"
                     /old_locus_tag="GCM10010166_50260"
     CDS             complement(311..1831)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="IEY99_RS24975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50260"
                     /product="MFS transporter"
                     /protein_id="WP_189206486.1"
                     /transl_table=11
                     /translation="MTTTIAAATAQGRPTRWLGFGALILAVLLVSVDGTVLALAMPSIG
                     RDLAATGTQMLWIGDVYSFVLAGLLVTMGTLGDRIGRRKLLLYGAAGFGLVSTAIAYAP
                     SAPWLIAGRVLLGVAGATLMPATLALIRTLFPDSRERSTAIGIWGAAMSAGAALGPVLG
                     GFLLEHFWWGSAFLINAPVMAILLAVGGKLLPESRNPEPGPWDLPSVALSMVGMLGVVY
                     AVKEIAAHGPGTGTVAAAMLGVAALLAFARRQLSLPHPLIDVRLFRNRTFGGVIAAGML
                     SVLGLSGLLYFLSQYFQLVVGYRPLEAGLAELPAALGSMLFGILAGATTRRTSVRTVLG
                     GGMALSGLAMATLTVVEASTPYLLLGTALFLMGAGMGLAFTVASDVILTVVPPHQAGAA
                     SAVSETAYELGMALGIATLGTVVTGAYRDAALPAGTPAHLAAAAHDSLAGAAAAAETLP
                     AAQAAALLDAARTAFTDGLALATTAGSILLLAAAAAVVALVRTPRPEK"
     gene            complement(1828..2367)
                     /locus_tag="IEY99_RS24980"
                     /old_locus_tag="GCM10010166_50270"
     CDS             complement(1828..2367)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.5"
                     /locus_tag="IEY99_RS24980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50270"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_229799215.1"
                     /transl_table=11
                     /translation="MKERDDILRAAAEFLGRRPNATQDEIARAAGVSRATLHRHFAGRT
                     ALFAALEQLAVAQMRQALATARLADGPATDALARLVAASQDASAYLALMYSQSQEYDPE
                     HPHEAWAEIDAEIAALFRRGQEAGEFRLDLGPAWLADAFYSLAAGAAWSVQTGRAAARD
                     FTHMVTTLLLHGAVRR"
     gene            complement(2420..3469)
                     /gene="mgrA"
                     /locus_tag="IEY99_RS24985"
                     /old_locus_tag="GCM10010166_50280"
     CDS             complement(2420..3469)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mgrA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080305.1"
                     /locus_tag="IEY99_RS24985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50280"
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="WP_189206487.1"
                     /transl_table=11
                     /translation="MIYAAAPDRYTSGMQYRRTGRSGLDLPVLSLGFWHNFGDDKPFEQ
                     QRAITRRAFDLGVTHFDLANNYGPPYGAAEVSFGRLMREDLRPYRDEIVVSTKAGWDMW
                     PGPYGRGGGGRKYVLASLDQSLGRMGLDYVDIFYSHRYDPTTPLEETASALDAAVRAGK
                     ALYVGISSYDAEHTRRMAGLLRELGTPLLIHQPSYSMLNRWIETEGLLDAAAEVGAGVI
                     GFTALAQGLLTGRYLNGIPEGSRATQGKSLDPGSIDEALVSRLRALDEVARARGQNLAQ
                     LALAWALRDPRVTSLVIGASSVRQLDENVATLGNLSFTDDELGLIDKILDADFSSVDLW
                     AGARQGKVM"
     gene            complement(3551..3979)
                     /locus_tag="IEY99_RS24990"
                     /old_locus_tag="GCM10010166_50290"
     CDS             complement(3551..3979)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014689422.1"
                     /locus_tag="IEY99_RS24990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50290"
                     /product="phage holin family protein"
                     /protein_id="WP_229799216.1"
                     /transl_table=11
                     /translation="MSDTRMRLDPSADASTGVLVQQMAEQVSTLVRDELALARTEMVEK
                     GKRAGAGAGMLGGAGVLALYGVSTLLLTIGAALALAWPAWLAALTVMVVVFAAAGVAAL
                     VGKKQVEQAVPPAPTAAAASAKRDVDTVATAVREGRRG"
     gene            complement(4140..4964)
                     /locus_tag="IEY99_RS24995"
                     /old_locus_tag="GCM10010166_50300"
     CDS             complement(4140..4964)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348413.1"
                     /locus_tag="IEY99_RS24995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50300"
                     /product="tyrosinase family protein"
                     /protein_id="WP_189206488.1"
                     /transl_table=11
                     /translation="MGVRKDQRNLTATEKKAFVDAVLELKRSGRYDAFVRTHNQFIVSD
                     TDNGNRVGHRSPSFLPWHRQFLLDFERALQSINPAVNLPYWNWTVDRTATASLFAADFM
                     GGTGRSSDGQVTTGPFAFSTGNWTINVRSDSRNYLRRALGAGGRSLPTATQLQTVMAQR
                     VYDVAPWNSSSTTGFRNALEGWRNGPAMHNLVHVWVGGDMGTGTSPNDPLFWLHHCHID
                     KIWSDWQRANPSSGYLPTGNTTNVVDLNDTMTPWGTVRPADMLNHTAWYTYE"
     gene            complement(5001..5423)
                     /locus_tag="IEY99_RS25000"
                     /old_locus_tag="GCM10010166_50310"
     CDS             complement(5001..5423)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.5"
                     /locus_tag="IEY99_RS25000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50310"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_189206489.1"
                     /sec_met_domain="melC (E-value: 4.1e-38, bitscore: 125.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNTKRRDLLKFAGAATVAAGTGALIVQVTTAGGSQAAVDAPVAA
                     AQPKQITETYRGRRIEIGAASPAQRQAAAHHLSATGGAADLGIRIDGVPLHVMQNADGT
                     YTSVSNHYESFKSLREVARAAVDELDGARLLPPQHG"
     misc_feature    complement(5506..5520)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.55"
                     /tool="antismash"
     misc_feature    complement(5603..5616)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 21.49"
                     /tool="antismash"
     gene            complement(5760..6386)
                     /locus_tag="IEY99_RS25005"
                     /old_locus_tag="GCM10010166_50320"
     CDS             complement(5760..6386)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441100.1"
                     /locus_tag="IEY99_RS25005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50320"
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="WP_189206490.1"
                     /transl_table=11
                     /translation="MTRILLISGDTREDSLHTAALRTAVRHAPAGIAVSLYDGLRALPA
                     FVPGEPVPPAAVALLRHRAGAAGAVLVSTPEYAGSLPGSLKNLLDWLVDSGELGGKPAA
                     WLSLSPPAQDQAALATLETVLEHARARVLKPACIRVPLGPRAVAGGVVTDPRLPTALQD
                     MLRALDRAVALPAPRRAPSWQVHSSVYPVVPRRDGAGFDPGRLGS"
     gene            6610..8007
                     /locus_tag="IEY99_RS25010"
                     /old_locus_tag="GCM10010166_50330"
     CDS             6610..8007
                     /NRPS_PKS="Domain: AMP-binding (12-374). E-value: 2.5e-62.
                     Score: 202.7. Matches aSDomain:
                     nrpspksdomains_IEY99_RS25010_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018738255.1"
                     /locus_tag="IEY99_RS25010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50330"
                     /product="acyl-CoA synthetase"
                     /protein_id="WP_189206491.1"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-70, bitscore:
                     231.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLLRALHDPADDERAVATPGLTLSRHDLLGRAAALAAELRGLPM
                     AAVHAVPAAPTVVAVTAGLLAGVPIAPVPPDAGDVERAHVLRDSGAAVMLSDDPGAAGS
                     SGLPVVPVSGPARGTVPAEPDAGAPALVLYTSGTTGAPKGVVLSRRAIAAGLDALAEAW
                     QWTAGDTLVHGLPLYHVHGLVLGVLGALRTGSRLVHTGRPTPQAYAQAAGSLYFGVPTV
                     WSRICAAPQEARALRGARLLVSGSAPLPVPVFTDLATLAGQAPVERYGMTETLITVSAR
                     ADGERRAGHVGLPVAGVQTRLAGDSGEPLPADGSTIGHLEVRGATLFDGYLRPDGTAAA
                     PLTADGWYRTGDVATVGPDGWHRIVGRASTDLIKTGGFRVGAGEVEDALLRHPAVREAA
                     VIGTPHPDLGEQVTAFVVADGRPDPDELTAFVAARLAAHKRPRLVRLVAELPRNAMGKV
                     QKARLRE"
     aSDomain        6646..7731
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS25010_AMP-binding.1"
                     /evalue="2.50E-62"
                     /label="IEY99_RS25010_AMP-binding.1"
                     /locus_tag="IEY99_RS25010"
                     /protein_end="374"
                     /protein_start="12"
                     /score="202.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="DERAVATPGLTLSRHDLLGRAAALAAELRGLPMAAVHAVPAAPTV
                     VAVTAGLLAGVPIAPVPPDAGDVERAHVLRDSGAAVMLSDDPGAAGSSGLPVVPVSGPA
                     RGTVPAEPDAGAPALVLYTSGTTGAPKGVVLSRRAIAAGLDALAEAWQWTAGDTLVHGL
                     PLYHVHGLVLGVLGALRTGSRLVHTGRPTPQAYAQAAGSLYFGVPTVWSRICAAPQEAR
                     ALRGARLLVSGSAPLPVPVFTDLATLAGQAPVERYGMTETLITVSARADGERRAGHVGL
                     PVAGVQTRLAGDSGEPLPADGSTIGHLEVRGATLFDGYLRPDGTAAAPLTADGWYRTGD
                     VATVGPDGWHRIVGRASTDLIK"
     CDS_motif       6997..7053
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS25010_0001"
                     /evalue="5.80E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="IEY99_RS25010"
                     /protein_end="148"
                     /protein_start="129"
                     /score="25.0"
                     /tool="antismash"
                     /translation="ALVLYTSGTTGAPKGVVLS"
     gene            8279..9226
                     /locus_tag="IEY99_RS25015"
                     /old_locus_tag="GCM10010166_50340"
     CDS             8279..9226
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622220.1"
                     /locus_tag="IEY99_RS25015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50340"
                     /product="DUF5995 family protein"
                     /protein_id="WP_189206492.1"
                     /transl_table=11
                     /translation="MTTTVAFDRGGVQSSAKLLREERRRIAPDEPGLVPAGRGWAPAAE
                     QMAELCADCPGTIGDVLRMLTRAQQIMNELPPPAANRVAAFNSLYFTITDRVEAALRGT
                     TVRDADFLELLDVEFAKRYFEALRLWGDENRATPDAWEILFRRAQDHRLSKLAAAMLGV
                     NAHINHDLALALIATWERLGPPRDDLVHPDYLLVNDIFYAEIPALRRRYSTSWQLRIDG
                     LVGDLDDWSQRVLVAATRARAWEQAERLWALRDDEEDLARALLVMDRASAYVGELLITG
                     DGVVNRVGAALRAGRDTVRTGVGRLLGRRSAAVR"
     gene            complement(9508..9999)
                     /locus_tag="IEY99_RS25020"
                     /old_locus_tag="GCM10010166_50350"
     CDS             complement(9508..9999)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622218.1"
                     /locus_tag="IEY99_RS25020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_50350"
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="WP_189206493.1"
                     /transl_table=11
                     /translation="MVLTFDPFRELDRLAGQVLGAAPGAGGAATAAMPMDLYRSGDHFV
                     LHCDLAGVDPGSVQVDVDNRILTIRAERSPRTDSDVQWLRRERVTGTFERRLTLGDGLD
                     LDRIAATWQDGVLTLTIPIAQAAKPRRIDISTTTGAPALKSESGPVVEGSAEERPAVSS
                     "
     misc_feature    10130..10139
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.77"
                     /tool="antismash"
ORIGIN
        1 gccgagcagc tcggcgccgt cccacacggt ccagaacgac acctcgggcg cccgcagctc
       61 cgtcaggtcc agggcgtgca cgctctccgc gggggaggtg gcgtgcatgt cggccagatg
      121 gtccgcgatc agccgccgga cctcctcccg gcccaggtcg tcgacctcga tcctcagctc
      181 cgccgcactc ccttgcgtga tcatgtgacg agtatgcggc acccacgcca cctgccggac
      241 cgctgcccgg gccgagccgt ccggtgcgca ggccggcggg tgcctgaacc ccggccagga
      301 cggttccggt ctatttctcg ggccgggggg tcctgacgag ggcgacgacg gcggccgcgg
      361 cggcgaggag caggatgctg ccggccgtcg tggccagggc gaggccgtcg gtgaaggcgg
      421 tccgggcggc gtccagcagc gccgcggcct gcgctgcggg cagtgtctcg gcggccgcgg
      481 cggcgccggc cagggagtcg tgcgcggccg cggcgaggtg ggccggcgtg ccggcgggca
      541 gggcggcgtc gcggtaggcg ccggtgacga ccgtgccgag agtggcgatg cccagggcca
      601 tgccgagctc gtaggcggtc tcggagaccg cggaggcggc gccggcctgg tgtggcggca
      661 cgacggtgag gatgacgtcg ctggccaccg tgaaggccag gcccatgccc gcgcccatga
      721 ggaacagcgc cgtgccgagc agcaggtacg gcgtggacgc ctcgaccacg gtcagcgtgg
      781 ccatggcgag gccgctgagc gccatgccgc cgccgagcac cgtgcgcacg gaggtgcggc
      841 gggtggtagc gccggccagg atgccgaaga gcatggagcc gagcgcggcg ggcagctccg
      901 cgaggcccgc ctcgaggggg cggtatccca cgacgagctg gaagtactgg gacaggaaat
      961 agagcagtcc ggagagcccc aggacggaga gcatgccggc cgcgatcacg ccgccgaagg
     1021 tgcggttgcg gaacagccgg acgtcgatga gcgggtgcgg caggctgagc tggcgccggg
     1081 cgaaggcgag gagggccgcc acgccgagca tggcggcggc caccgtgccg gtgcccggcc
     1141 cgtgcgcggc gatctccttg acggcgtaga ccacgccgag catgccgacc atggacagtg
     1201 cgacgctggg caggtcccac ggcccgggct cggggttcct ggactcgggc agcagcttgc
     1261 cgccgaccgc cagcaggatc gccatgaccg gcgcgttgat gaggaaggcg gagccccacc
     1321 agaagtgctc gagcaggaac ccgccgagca cggggccgag cgccgcgccc gcggacatgg
     1381 cggcgcccca gatgccgatc gccgtgctgc gttcccggct gtccgggaag agcgtacgga
     1441 tcagcgccag cgtggcgggc atcagcgtgg cgccggcgac gcccagcagg acgcggcccg
     1501 cgatcagcca cggtgcgctg ggggcgtacg cgatggccgt ggagacgagg ccgaaccccg
     1561 cggccccgta cagcaggagt ttccggcggc cgatccggtc gccgagggtg cccatggtga
     1621 ccagcaggcc ggcgaggacg aaggagtaga cgtcgccgat ccacagcatc tgcgtgccgg
     1681 tcgcggcgag gtcgcggccg atggacggca tcgccagggc caggacggtg ccgtcgaccg
     1741 agacgaggag caccgcgagg atcagcgcgc cgaagccgag ccagcgcgtc ggccggccct
     1801 gcgcggtggc ggcggcgatg gtggtggtca tcggcgcacc gcgccgtgca gcaggagcgt
     1861 ggtgaccatg tgggtgaagt cgcgggcggc ggcccgtccg gtctgcacgg accacgccgc
     1921 gccggccgcc aggctgtaga acgcgtcggc cagccaggcc gggccgaggt cgagccggaa
     1981 ctcgccggcc tcctgccccc gccggaagag cgcggcgatc tccgcgtcga tctcggccca
     2041 ggcctcgtgc gggtgctcgg ggtcgtactc ctggctctgg ctgtacatca gcgccaggta
     2101 ggcggacgcg tcctggctgg ccgccacgag gcgggccagg gcgtcggtcg ccgggccgtc
     2161 cgcgagccgg gcggtggcca gcgcctgccg catctgtgcc acggcgagct gttcgagggc
     2221 cgcgaagagc gccgtccgtc cggcgaagtg ccggtgcagc gtcgcccggc tcaccccggc
     2281 ggccctggcg atctcgtcct gggtggcgtt ggggcggcgg cccaggaact cggccgccgc
     2341 ccgcaggatg tcgtcccgtt ccttcacgag acgaagatag ctcagatgag acagaaacgt
     2401 ctcacctgag tgcgtgcggt cacatgacct tgccctgccg ggcgccggcc cagagatcca
     2461 cggaggagaa gtcggcgtcg aggatcttgt cgatcaggcc gagctcgtcg tcggtgaagg
     2521 agaggttgcc gagcgtggcc acattctcgt cgagctgccg cacgctgctg gcgccgatca
     2581 ccagcgaggt gacgcgcggg tcgcgcagcg cccaggcgag cgcgagctgg gccaggttct
     2641 gcccgcgggc ccgggcgacc tcgtcgagcg cgcgcagccg cgagaccagc gcctcgtcga
     2701 tcgatcccgg gtccagcgac ttgccctggg tggcgcgcga gccctccggg atgccgttca
     2761 ggtacctgcc ggtgagcagc ccctgggcca gcgcggtgaa gccgatcacc ccggcgccca
     2821 cctcggccgc cgcgtcgagc aggccctcgg tctcgatcca gcggttcagc atcgagtacg
     2881 acggctggtg gatgagcaac ggcgtgccca gctcgcgcag cagcccggcc atccggcggg
     2941 tgtgctcggc gtcgtacgag gagatgccca cgtagagggc cttgccggcg cggaccgcgg
     3001 cgtccagcgc cgaggcggtc tcctccagcg gcgtcgtggg gtcgtagcgg tgcgagtaga
     3061 agatgtcgac gtagtccagg cccatccggc ccagcgactg gtcgaggctg gcgagtacgt
     3121 acttgcgtcc gccgccgccc cggccgtacg ggcccggcca catgtcccag ccggccttcg
     3181 tcgagaccac gatctcgtcc cggtacggcc gcaggtcctc gcgcatcagc cggccgaagc
     3241 tgacctcggc ggcgccgtac ggcgggccgt agttgttggc gaggtcgaag tgcgtgacgc
     3301 cgaggtcgaa ggcccgccgg gtgatggcgc gctgctgctc gaacggcttg tcgtcgccga
     3361 agttgtgcca gaaaccgagc gagagcacgg gcaggtcgag gcccgaacgc ccggtgcggc
     3421 ggtactgcat tccactggtg tatcggtccg gtgcggcggc gtagatcatg acgccactat
     3481 cccggccgtg atcatggacg tccaccaccg gcggcggacg ccgccggtgt gtcccccggt
     3541 cgcgcgcggc tcacccgcgg cgaccctcgc gcacggccgt cgcgacggtg tccacgtcac
     3601 gcttggcgct ggccgccgcg gccgtcggcg cgggcggcac cgcctgctcc acctgcttct
     3661 tgccgaccag ggcggcgacg cctgccgccg cgaagaccac gaccatgacc gtaagggcgg
     3721 ccagccaggc gggccaggcc agcgccagcg cggcaccgat cgtcagcagc agggtggaga
     3781 cgccgtagag ggcgaggacg ccggccccgc cgagcatccc ggccccggct ccggcccgct
     3841 tgcccttctc caccatctcc gtacgcgcga gcgccagctc gtcgcgcacg agggtggaga
     3901 cctgctcggc catctgctgg acgagcaccc ccgtggaggc gtccgccgag gggtccagcc
     3961 tcatccgtgt gtcgctcatg gccaccactt accccagctc aggcgggtta tgcggccgga
     4021 aggctcaggt agcccgatcg gctgccgatg gctgcgctca cggaagggga tgatcaaggt
     4081 ggggatcccg gcccgggcaa ctggcgtcgc ccgggccggt gccactcatc cggcacgggt
     4141 cactcgtagg tgtaccaggc cgtgtggttc agcatgtcgg ccggccgcac ggtgccccac
     4201 ggggtcatgg tgtcgttgag gtccacgacg ttggtggtgt tgccggtcgg caggtagccg
     4261 gagctcgggt tcgcccgctg ccagtccgac cagatcttgt cgatgtggca gtggtgcagc
     4321 cagaacagcg ggtcgttggg cgaggtgccg gtgcccatgt cgccgcccac ccagacgtgc
     4381 acgaggttgt gcatggccgg accgttccgc cagccctcca gcgcgttgcg gaagccggtg
     4441 gtggagctgc tgttccaggg cgcgacgtcg tacacccgct gcgccatgac cgtctgcagc
     4501 tgggtggccg tcggcaggct gcgcccgccg gcacccagcg cgcggcgcag gtagttacgg
     4561 ctgtcgctgc ggacgttgat cgtccagttg ccggtgctga acgcgaacgg gccggtggtc
     4621 acctgaccgt ccgagctgcg tccggtgccg cccatgaagt ccgccgcgaa cagcgacgcg
     4681 gtggcggtgc ggtcgacggt ccagttccag tacggcaggt tcaccgccgg gttgatcgac
     4741 tgcagcgccc gctcgaagtc gagcaggaac tgccggtgcc acggcagaaa cgacggcgag
     4801 cggtggccga cgcggttgcc gttgtcggtg tcgctgacga tgaactggtt gtgggtacgg
     4861 acgaaagcgt cgtagcggcc gctgcgcttg agctcaagca cggcgtcgac gaacgccttc
     4921 ttctccgtgg cggtcaggtt gcgctggtcc ttgcgtacgc ccatgagtgg tgcagtcgcc
     4981 tttcgggtaa gtaggaggga tcagccgtgc tgcggcggca gcaggcgggc accgtcgagc
     5041 tcgtcgacgg cggcgcgggc gacctcgcgc agggacttga agctctcgta gtggttcgac
     5101 acgctcgtgt aggtgccgtc ggcgttctgc atcacgtgca gcgggacgcc gtcgatgcgg
     5161 atgccgaggt cggccgcgcc acccgtggcg gacaggtggt gggctgcggc ctgcctctgc
     5221 gccggcgagg cggcgccgat ctcgatccgc cggccccggt aggtctcggt gatctgcttc
     5281 ggctgcgcgg cggcgaccgg cgcgtcgacg gcggcctgcg agccaccggc ggtggtgacc
     5341 tgcacgatga gtgctcccgt gccggcggcc acggtggcgg cgccggcgaa cttgagcaga
     5401 tcgcgacgtt tggtgttgga catttcctcc cccttctccg gacgccgggc agcaaaaatt
     5461 cacgccacaa aggaagatga acgtaaaaat tgctgcgctg ctcggcattt cctgagtatt
     5521 tccggcgccg gaagcggggc gaaggtaaca gcggcgctcg gcgccgggca aggcgctcga
     5581 tgcaagcagt cacggtgcag attcgcgcag agtgactctg cacggcccgt gcaccgggca
     5641 ccgcacgggg atcggcctcg ataagatcaa taagtgatcg tcaacggtcg atcaccggcg
     5701 tgcgcgctgc gtcatcgccg gtccgccggg gtgagctggt tcatagccga acggacagct
     5761 cagctgccga gtcggccggg gtcgaagccg gcaccgtcgc ggcgcggcac gaccggatag
     5821 acgctcgagt gcacctgcca ggacggagcc cgccgcggcg cgggcagcgc gaccgcccgg
     5881 tccagcgcgc gcagcatgtc ctgcagcgcg gtcggcagcc gcgggtcggt gaccacgccg
     5941 ccggcgacgg cgcgcgggcc gagcgggacg cggatgcacg ccggcttgag cacccgggcc
     6001 cgggcgtgct ccagcaccgt ctccagcgtc gccagggccg cctggtcctg cgccggcggg
     6061 gagagcgaga gccacgcggc cggcttgccg ccgagctcgc cgctgtcgac gagccagtcc
     6121 aggaggttct tcaggctgcc cggcagcgag ccggcgtact cgggggtgct caccagcacg
     6181 gccccggccg cgccggcccg gtgtcgcagc agggccaccg cggcgggcgg gaccggctcg
     6241 ccggggacga acgcgggcag tgcgcgcagg ccgtcgtaga gactcacggc gatgcccgcg
     6301 ggcgcgtgcc gcaccgccgt gcgcagcgct gcggtgtgca gggagtcctc tcgcgtgtca
     6361 cccgagatca gcaggatgcg cgtcatgacg ccgacgatag ggcccccggc cgcgcacggc
     6421 cgggcccgcg cccgcgacgc cgggcccggc gtcgagtcgg atcgaggacg tttcccgggt
     6481 gaaaccgcgg cacctccagg ccctccaccg agcgcaaggg cgccacccgc catgccgtga
     6541 ccgggcactg ggcgccgcgt ggcggggtgg ccgtcgtcga tggccggcgc ggtctgcgat
     6601 catcgcggca tgcgcctgct gcgagctctg cacgaccccg cggacgacga gcgggccgtc
     6661 gccacgcccg ggctgacgct gtcccggcac gatctgctgg gccgtgccgc cgcgctcgcc
     6721 gccgagctgc gggggctgcc gatggcggcc gtgcacgcgg tccccgccgc gccgacggtc
     6781 gtggccgtga ccgccgggct gctggccggg gtgccgatcg ctccggtgcc acccgacgcc
     6841 ggcgacgtgg aacgcgcgca cgtcctgcgg gactccggcg cggccgtgat gctctccgac
     6901 gatcccgggg ccgccgggag cagcgggctg cccgtcgtgc cggtctccgg gcccgcgcgc
     6961 gggaccgttc ccgccgagcc ggacgccggc gccccggccc tcgtgctcta cacgagcggg
     7021 acgaccggcg cgccgaaggg cgtcgtgctg tcgcgccggg cgatcgccgc cgggctcgac
     7081 gcgctcgccg aggcatggca gtggacggcc ggcgacactc tcgtgcacgg gttgccgctc
     7141 taccatgtgc acgggctcgt gctcggcgtg ctcggcgcgc tgcggaccgg gtcgcggctc
     7201 gtgcacaccg gacggcccac cccgcaggcg tacgcgcagg ccgcgggcag cctctacttc
     7261 ggagtgccca ccgtctggtc gcgaatctgc gccgccccgc aggaagcgcg cgccctgcgg
     7321 ggagcgcggc tgctcgtgtc gggcagcgcg ccgctgccgg tgccggtctt caccgacctg
     7381 gccaccctgg ccgggcaggc gccggtggag cggtacggca tgaccgagac cctcatcacg
     7441 gtcagcgccc gggcggacgg cgagcggcgc gcgggccacg tcggcctgcc cgtcgccggc
     7501 gtgcagaccc gcctggccgg tgactcgggc gagccgctgc cggccgacgg cagcaccatc
     7561 ggccacctcg aggtccgcgg cgccacgctc ttcgacggct acctgcggcc ggacggcacc
     7621 gcggccgccc cgctcaccgc cgacggctgg taccgcaccg gcgacgtcgc gaccgtcggg
     7681 cccgacggct ggcaccgcat cgtcggccgc gcctccaccg atctgatcaa gaccggcggg
     7741 ttccgggtcg gcgccggtga ggtcgaggac gcgctgttgc ggcaccctgc cgtccgggaa
     7801 gccgcggtga tcgggacccc gcacccggac ctgggggagc aggtgaccgc cttcgtggtc
     7861 gccgacggcc gacccgaccc ggacgagctg acggcgttcg tggccgcccg gctggccgcc
     7921 cacaagcgtc cccggctggt gcgcctcgtc gcggaactgc cccgcaacgc catgggcaag
     7981 gtccagaagg cgcgcctgag ggagtgagaa ggttccggac cgggccggcg ggcggcaccg
     8041 gcgagcacgg tggtgccacc cgagtcgccg ccatgctccg cggtcgcggg ggccccaccg
     8101 gtcgcgaccg ccgtggtagt ctccggcggc gccatcgatg tggcccgatt caataatctg
     8161 ctttttcgct gaccgcgacg ctactagtgt cgggtagccg ccgggaagct ggtcaatcac
     8221 ggtgatcttc agcactgtgg acgaccgacg gggaagctac gggggctggg gggccaccat
     8281 gaccacgact gtcgccttcg accgcggtgg agtgcagagc agcgccaagc tgttgcgcga
     8341 ggaacgccgg cggatcgcac cggacgaacc gggcctggtg cccgccggcc gcggctgggc
     8401 gcccgccgcc gagcagatgg ccgagctgtg cgccgactgc ccgggcacca tcggcgacgt
     8461 gctgcggatg ctcacccggg cgcagcagat catgaacgag ctgccgccac ccgcggccaa
     8521 ccgggtcgcc gcgttcaact cgctctactt cacgatcacc gaccgggtcg aggcggcgct
     8581 gcgcggcacg acggtgcgcg acgccgactt cctcgaactg ctcgacgtgg agttcgccaa
     8641 gcgctacttc gaggcgctgc gcctctgggg cgacgagaac cgcgccaccc cggacgcctg
     8701 ggagatcctc ttccggcggg cccaggacca ccggctcagc aagctcgccg ccgcgatgct
     8761 gggcgtgaac gcgcacatca accacgacct ggcgctggcc ctgatcgcca cgtgggaacg
     8821 cctcggcccg ccgcgcgacg acctggtcca cccggactac ctgctggtca acgacatctt
     8881 ctacgccgag atcccggccc tgcgccgccg ctactcgacg agctggcagc tgcgcatcga
     8941 cggcctggtc ggcgacctgg acgactggag tcagcgggtc ctcgtggccg cgacccgcgc
     9001 ccgcgcctgg gagcaggccg agcggctctg ggcgctgcgc gacgacgagg aggacctggc
     9061 gcgggccctg ctcgtgatgg accgcgcctc ggcgtacgtg ggggagctgc tgatcaccgg
     9121 cgacggcgtg gtgaaccggg tgggcgcggc actgcgcgcc ggccgcgaca ccgtgcggac
     9181 gggcgtcggc cgcctgctgg gccgccggag cgccgcggtc cgctgagcgc aagcgccgcc
     9241 gagcgccgcg gcccgctgag cgcaagcgcc gccggagcgc cgcggcccgc tgagcgcaag
     9301 cgccgccgga gcgccgcggc ccgctgagcg caagcgccgc cggggcctcg tgcggaagtg
     9361 gccgccgggg cgtcgcggtg cgctgagggc aggggcgcgt tcgtcgaccg tgggggcccg
     9421 ccgggcgcgg aagtggagga ttccgcgccc ggcgaggcca cccgtgcatg ccccgcagcg
     9481 gcgaaccgcc ggctccgggg cggcgggtca cgacgagacg gccggcctct cctcggcgct
     9541 gccctcgacg accgggccgc tctcgctctt cagcgccggc gcgccggtcg tggtggagat
     9601 gtcgatgcgg cgcggcttgg cggcctgcgc gatcgggatg gtgagcgtca gcacgccgtc
     9661 ctgccaggtc gccgcgatgc ggtcgaggtc gagaccgtcg ccgagggtca ggcggcgctc
     9721 gaaggtgccg gtgacgcgtt cgcggcgcag ccactggacg tcggagtcgg tgcgcggcga
     9781 gcgctcggcg cggatggtga ggatgcggtt gtcgacgtcg acctggaccg agccggggtc
     9841 gacaccggcc aggtcacagt gcaggacgaa gtggtcaccg gagcggtaga ggtccatcgg
     9901 catcgcggcg gtcgcggccc cgccggcccc gggggcggcg ccgagaacct ggccggccag
     9961 gcggtcgagc tcgcggaaag gatcgaaggt cagcaccaca atccacctcc tcgtgcggcc
    10021 cgccggacga tccggcgggc atgtggcgct cggcgatctg atcttctctg ccgaggtcgc
    10081 acagcctgcc ccgaggtggt catcaccgcg gttgagctgc agcgacagtt cattttttag
    10141 cactgtcccc tggcgagtgc caagaggcgc cgcaagattt ttctgcgagg atggcgccat
    10201 gcccgctctc gtcgaccgct ccggccaccg ctacccgatc gatgagccgc ggtggcgcgg
    10261 cgacgacggt tcgccgctca tggtggcgcc gctgcccggc atcacccgcg ccgacgtcga
    10321 cacctccgtg cgctcgctgt ggcgctatgc cgccgccctg ccggcaccga tcgcgcgccc
    10381 ggtgaccctc ggcgaggggc ggacgccgct gctgcccctc gag
//
