LOCUS       NZ_BMQY01000004        10435 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Couchioplanes caeruleus subsp. azureus strain JCM 3246 sequence04,
            whole genome shotgun sequence.
ACCESSION   NZ_BMQY01000004
VERSION     NZ_BMQY01000004.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Couchioplanes caeruleus subsp. azureus
  ORGANISM  Couchioplanes caeruleus subsp. azureus
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Couchioplanes.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 770393)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 64X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,801
            CDSs (total)                      :: 6,731
            Genes (coding)                    :: 6,630
            CDSs (with protein)               :: 6,630
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 20 of 101
            Pseudo Genes (incomplete)         :: 93 of 101
            Pseudo Genes (internal stop)      :: 0 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:23:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 101298
            Orig. end                         :: 111733
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMQY01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10435
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10435
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10435
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5435](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5435)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(533..1948)
                     /locus_tag="IEY99_RS15565"
                     /old_locus_tag="GCM10010166_31250"
     CDS             complement(533..1948)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510582.1"
                     /locus_tag="IEY99_RS15565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31250"
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="WP_189203811.1"
                     /transl_table=11
                     /translation="MTAVLARPAPPATRPLAAVLAAVGIPTFMVTLDNLVVTTALPVIR
                     TELGASLTDLQWFVNAYTLPFAAFLLTAAAVGDRLGRRRMFVAGIVVFTLASAAAALAG
                     EPWMLTAARAVQGLAGAAVAPLSLTLLAQAVPERLRNAAVGIWGGINGLGVAVGPVVGG
                     AVVEGLHWSWIFWLNVPVGALAVVLATTVLRESRGGAPRLDPLGLLLSAAGMLSLVWGV
                     VDGPDHGWTSARVLGMLGAGGVLLALFVAWQRRNPTPMLPLTLFRSRGFGLVSVVTLTF
                     SAGTFGAVFLLAQFFQVVQGLSPLASGVRTLPWTMAPMVVAPLAGIFADRLGLRNLIVA
                     GQALLAAGVLWIAVATTTTVSYGDLVTAFVLAGVGMGLTFAPISTMALASVRPEQNGVA
                     SGANNTIRELGIAVGVAVLASVFSEYGGYTSAQSFVDGIVPAVLVGAAVIAAGAAVATL
                     LPRRPAGTPPRAQ"
     misc_feature    1962..1979
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.3"
                     /tool="antismash"
     misc_feature    1966..1980
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.8"
                     /tool="antismash"
     gene            2034..2576
                     /locus_tag="IEY99_RS15570"
                     /old_locus_tag="GCM10010166_31260"
     CDS             2034..2576
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622678.1"
                     /locus_tag="IEY99_RS15570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31260"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_189203813.1"
                     /transl_table=11
                     /translation="MARTRLTAEQRQEQLLRAALTAFSTGGYAGTTTDQVARLAGVSQP
                     YVIRIFGTKQQLFLATVRRAGDRIEQRFRQAAAEDPTLASLGAAYDDLLAERELITVLL
                     HGFTASADPAIGPVVRDCFGRIYRTVRELTGAGEEEARGFLAMGMLLTCLGAMRVVGPD
                     AVPREPWMTELLGGDGS"
     gene            complement(2573..3457)
                     /gene="folP"
                     /locus_tag="IEY99_RS15575"
                     /old_locus_tag="GCM10010166_31270"
     CDS             complement(2573..3457)
                     /EC_number="2.5.1.15"
                     /GO_function="GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="folP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622679.1"
                     /locus_tag="IEY99_RS15575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31270"
                     /product="dihydropteroate synthase"
                     /protein_id="WP_229798751.1"
                     /transl_table=11
                     /translation="MSDYAPPLISAERVIGGRRYDFSRRVAVMAIVNRTPDSFHDRGRT
                     FALDRATEAVRAAAEAGADWIDIGGVPFAPGPEVSASEELDRVLPVVEAAQGLAVVSVD
                     TFRPEVAREVVKAGAGVVNDTSGLRDPAMADVVAGTDAALVITHSLAAPRTPYPGPTYG
                     DVTAEVADFLRRRVQLALDRGVRPEQIIIDPGHDLNKNTLHTLEVTRRLHEIADLGHPL
                     LAAVSNKDFIGETLDLPQRERLSGTLAAVVFCILRGARIVRVHDVRPAVEAVRMTEAML
                     GLREPAYTRHNMP"
     misc_feature    3423..3436
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.28"
                     /tool="antismash"
     gene            3606..4103
                     /locus_tag="IEY99_RS15580"
                     /old_locus_tag="GCM10010166_31280"
     CDS             3606..4103
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEY99_RS15580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010166_31280"
                     /product="hypothetical protein"
                     /protein_id="WP_189203815.1"
                     /transl_table=11
                     /translation="MWAWLRGRVLAPVDIGFIADRSAVAYEGAALAGEPAVVAALARAP
                     EVFLAVADDPGNAEMAVDREDLPGLLEVVTADGRAWELLIDCTVKGIVDTGGYTGVEDD
                     LVELALARGPGVISTAHLEREVYWLRLTRPEPPERVLARFVGAVVDAHRELARRLDIAL
                     PG"
     misc_feature    3643..3657
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.13"
                     /tool="antismash"
     gene            complement(4140..4946)
                     /locus_tag="IEY99_RS15585"
                     /old_locus_tag="GCM10010166_31290"
     CDS             complement(4140..4946)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691977.1"
                     /locus_tag="IEY99_RS15585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31290"
                     /product="tyrosinase family protein"
                     /protein_id="WP_189203817.1"
                     /transl_table=11
                     /translation="MAVARKSVAALTSTEKTAFVNALRQFKTTSANGRNYNWYIDQHIA
                     FFARTSDNISHAHQSPSFFPWHREYLRLLEFDLQTVSGNPDIFIPYWDWTGTATPFTTD
                     FCGAITSGSVSSGNFSPAWGWNIYRSSIATSYLQRRLGQNATRPSASTVSTVQSYTVYD
                     QWPWNSTVSNSYRNRNEIDLHNRVHNYVGGHMGTREAPNDPVFWLHHCNVDRLWWLWQT
                     SRGVDTYQPRTGTSGVIDNTETMRPFASGRTPSAVRDISTLNYSYA"
     gene            complement(5001..5435)
                     /locus_tag="IEY99_RS15590"
                     /old_locus_tag="GCM10010166_31300"
     CDS             complement(5001..5435)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017995.5"
                     /locus_tag="IEY99_RS15590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31300"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_189203819.1"
                     /sec_met_domain="melC (E-value: 6.2e-14, bitscore: 47.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRRDVLRYGAAAAVSATTVAVATQTVTAARSDAAPQRAAADPRD
                     FDEVYKGKKIKGKHHSNGKHELHINNKKLALTPIDTLFVPEDGSAPHVGVGYISALNHY
                     DPVEIDESQHKDGLKKLAKKAVDTLGEFELGDEAGLAHHH"
     misc_feature    5489..5503
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.93"
                     /tool="antismash"
     misc_feature    complement(5507..5527)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.67"
                     /tool="antismash"
     misc_feature    complement(5517..5533)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.1"
                     /tool="antismash"
     gene            5676..6071
                     /locus_tag="IEY99_RS15595"
                     /old_locus_tag="GCM10010166_31310"
     CDS             5676..6071
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEY99_RS15595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010166_31310"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_189203821.1"
                     /transl_table=11
                     /translation="MRAIPILAAVLAAGAGLIATAGPAVAAPAPQPAAQAGADLAGFFA
                     QRYGGHHIMGWGRDESACSYIDGVQLVLYPLGRNRYVSALQAYEEERGVKNITKASADV
                     LGEARLVPPADPVAHCPVFVTAAPEGS"
     misc_feature    complement(6182..6195)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.73"
                     /tool="antismash"
     misc_feature    6277..6290
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.15"
                     /tool="antismash"
     misc_feature    6277..6291
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.87"
                     /tool="antismash"
     misc_feature    complement(6278..6291)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.46"
                     /tool="antismash"
     gene            6379..7281
                     /locus_tag="IEY99_RS15600"
                     /old_locus_tag="GCM10010166_31320"
     CDS             6379..7281
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622674.1"
                     /locus_tag="IEY99_RS15600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31320"
                     /product="hypothetical protein"
                     /protein_id="WP_189203822.1"
                     /transl_table=11
                     /translation="MSDPYTGARAPDATPGYGEVPVQPVVEAYPATTGPATGTGLTTGD
                     DRAFGASSSGGGTAEGAKEQAKHVAGEGAHAAGQAAGEIKDTAVEQAQRVGAEAKTQMR
                     NVASDVRDRVSTQARDQNDKLVGTLRQAADQLDEMRGERQDSPAAQVVSRIAEGGRQMA
                     DYLDRNGPEGVLREVTDFARRRPGAFLATALAAGFVAGRLGKSVMKADDTAGKPRTDTF
                     VSGADAGYGSGFADTTAGYPAAGTTTGYPAAGATAGYPSAGTTAGYPAAGTTAAGTEYA
                     STGTGTPAVVDEQYPGGTR"
     gene            7278..7700
                     /locus_tag="IEY99_RS15605"
                     /old_locus_tag="GCM10010166_31330"
     CDS             7278..7700
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622673.1"
                     /locus_tag="IEY99_RS15605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31330"
                     /product="phage holin family protein"
                     /protein_id="WP_189203823.1"
                     /transl_table=11
                     /translation="MTAPYPTGHDRAEEVSATSVGELIGNISNDLSQLFRQEVELAKAE
                     LKQEAGKAGKAAGMLGGAGFAGYLAVVLLSLALVFGLGNVMDLGWAALIVAVIWGAAGA
                     VLYAAGRKRLKSVDPVPRRTVDTIKEDAQWLKNPTG"
     gene            7676..8314
                     /locus_tag="IEY99_RS15610"
                     /old_locus_tag="GCM10010166_31340"
     CDS             7676..8314
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622672.1"
                     /locus_tag="IEY99_RS15610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31340"
                     /product="DUF3618 domain-containing protein"
                     /protein_id="WP_189203824.1"
                     /transl_table=11
                     /translation="MAEEPDRLRQEIEQTRASLTRDVDLLAEKTSPKQVARRRWTAVKE
                     KVMGTTEHARHSSSGAAHAAASTVQDKASRLGDKASELSDRAGEKAHDVADAVKGAPQA
                     MTQQAQGNPLAAGIIAFGVGMLAATLIPVTEAEKRAGEQLKEHGGEVTDRVKDVAMELK
                     DEVTGTVQQAAGQLKETARDAAETTKDQARSSAQDAKEQSKQAVADARS"
     gene            8385..9341
                     /locus_tag="IEY99_RS15615"
                     /old_locus_tag="GCM10010166_31350"
     CDS             8385..9341
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622671.1"
                     /locus_tag="IEY99_RS15615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31350"
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="WP_189203825.1"
                     /transl_table=11
                     /translation="MRIGYKLASEGFGPKELIAQAVAAERAGFDFVEISDHYHPWLDAQ
                     GHSPFAWTVLGAIAARTDRIGLATGVTCPSVRYHPAIIAQAAATLAITSDHRFTLGVGS
                     GERLNEHVVGQGFGNVRERQERLREALEIIKLLWRGGYQSYEGKHLRLEDARVFDLPDE
                     PPVIAVAAGGRDAATMAAELGDGLFATEPRADLVETYRGAGGAGPRYAEVPVAWAPTAE
                     EGIREAHRTTRWALTGWKVMSELPNPVNFEAASQTVREEDVREKFAAGPDPEPYLEAVR
                     TYVAAGFDHIVLQNAGPDPDGFIDFFQKELGDRLRSA"
     gene            9404..10264
                     /locus_tag="IEY99_RS15620"
                     /old_locus_tag="GCM10010166_31360"
     CDS             9404..10264
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510846.1"
                     /locus_tag="IEY99_RS15620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_31360"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_189203826.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 9.2e-15,
                     bitscore: 50.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMIQTGRAAAPVPPETADVTVVVATRNRRDRLRETLPRHAAPVV
                     LVDNGSDDGGPDAVAGVDVVRLGENRGAAARTVGVERAATKYVAFADDDSWWEPGSLAR
                     AAALLDAYPRTALISARVLVGRQRREDPISAAMAAAPLGVRDGAPGPEILGFLACAVVL
                     RRDAYLRAGGFHPALHVYGEEALLAMDLAAAGHRLAYAPSLTVRHMPLPAGRDTGLRHR
                     REVRNRVLTALLRRPPAVVGRTVAAAWRADRAGTVDALRMLPRILGQRRTLPQDVERDL
                     RVLGC"
ORIGIN
        1 cggaacgacc tgtccccggg tggtgctgca ggccgcggac gcaccggacc tcaacccggg
       61 cagcaagaac ctgaagtacg gcgcgtacgt cctgctctcc ccggcggaga cctcgacggg
      121 agagaacatc atgcagaagg gctactcgac cgccggcggc cagtacaaga tgcagatcga
      181 cggcgtgtcc ggcaagccga gctgcgtgat gagcgacaag gaggccacga ccatccacgt
      241 cgccaagagc ggcaggtcga tggcggacgg ctcctggcac accatcgagt gccgccgggc
      301 cggcaccacg ctgaccatcc tggtggacga caaggtggag ggcagcacga cgctgcccgc
      361 ggccctgtcg gtgatcacca cccagccgct cagcctgggc ggcaagggta cgggcgtcaa
      421 caacgaccag ttccacggca gcctcgacga cgtgtgggtc agcatcggct gaaccccgga
      481 gacgacgacg gccgggaccc ggtgtcccgg ccgtcgtggc gtctgcgggc agtcactgcg
      541 cgcgcggtgg tgtccctgcc gggcgccggg ggagcagggt ggccaccgcc gcaccggccg
      601 cgatcacggc cgcgccgacc aggacggccg ggacgatgcc gtcgacgaag gactgtgccg
      661 aggtgtagcc gccgtactcg ctgaagaccg aggccaggac cgcgacgccg accgcgatgc
      721 ccagctcgcg gatggtgttg ttggcgccgg aggcgacccc gttctgctcg ggccgcacgc
      781 tcgccagggc catcgtgctg atcggcgcga aggtcaggcc catgccgacg ccggccagga
      841 cgaaggcggt caccaggtcg ccgtaggaga cggtggtcgt ggtcgccacg gcgatccaga
      901 gcacgcccgc ggcgaggagc gcctgcccgg cgacgatgag gttccgcagc ccgaggcggt
      961 cggcgaagat gcccgccagc ggtgcgacga ccatcggggc catggtccag ggcagcgtgc
     1021 gcacccccga ggccagcggg ctgagcccct ggacgacctg gaagaactgg gcgagcagga
     1081 acaccgcgcc gaacgtgccc gccgagaagg tcagggtgac cacgctgacc agaccgaacc
     1141 cgcgggagcg gaacagggtg aggggcagca tcggcgtggg gttgcgccgt tgccaggcga
     1201 cgaagagggc gagcaggacg ccgccggcgc cgagcatgcc gagcacccgg gccgacgtcc
     1261 agccgtggtc cggcccgtcc accacccccc agaccagcga cagcatgccg gccgcggaca
     1321 gcagcaggcc gagcgggtcg agccggggcg cgccgccgcg ggactcgcgc agcacggtgg
     1381 tcgccaggac gacggcgagc gccccgacgg gcacgttgag ccagaagatc cacgaccagt
     1441 gcaggccctc gaccacggcg ccgccgacga ccgggccgac ggcgacgccg aggccgttga
     1501 tgccacccca gatgccgacc gcggcgttgc gcaggcgctc gggtacggcc tgggccagca
     1561 gcgtgagcga caggggtgcg acggccgcgc cggccagccc ctgcaccgcc cgcgcggcgg
     1621 tgagcatcca gggctcgccg gccagcgcgg cggcggcgga cgccagggtg aagacgacga
     1681 tcccggcgac gaacatccgc cggcggccga gccggtcgcc cacggcggcg gcggtgagca
     1741 ggaaggcggc gaagggcagc gtgtacgcgt tgacgaacca ctgcaggtcg gtgagggagg
     1801 cgccgagctc ggtgcggatc accggcagcg ccgtggtgac cacgaggttg tcgagggtga
     1861 ccatgaaggt cgggatgccg acggcggcca gcaccgcggc cagcgggcgg gtggccgggg
     1921 gtgccggccg ggcgaggacg gcggtcatcg cgcgctccta gttgttattc actgataact
     1981 agcgaccgcc accttagtta tcaaccgatg ccttggcaag gtagcgtcgg gacgtggccc
     2041 gcacccgcct gaccgccgaa caacgccagg agcagctgct ccgcgccgcg ctgaccgcct
     2101 tctccacggg cggctacgcc ggcaccacca ccgaccaggt cgcccggctg gccggcgtct
     2161 cccagccgta cgtgatcagg atcttcggca ccaagcagca gctcttcctc gccaccgtgc
     2221 ggcgcgcggg cgaccgcatc gagcagcggt tccggcaggc cgccgccgag gaccccaccc
     2281 tggccagcct cggcgccgcc tacgacgacc tgctggccga gcgggagctg atcaccgtgc
     2341 tgctgcacgg cttcaccgcg agcgccgacc ccgcgatcgg gccggtggtg cgcgactgct
     2401 tcggccggat ctaccgcacg gtccgggagc tgaccggcgc cggcgaggag gaggcccgcg
     2461 ggttcctcgc gatggggatg ctgctcacct gcctgggggc gatgcgggtg gtgggcccgg
     2521 acgcggtgcc ccgggaaccg tggatgaccg agctgctcgg cggcgacggg tcctagggca
     2581 tgttgtgccg ggtgtacgcc ggctcgcgca gcccgagcat cgcctcggtc atccggaccg
     2641 cctcgaccgc cgggcgcacg tcgtgcacgc ggacgatgcg ggcgccgcgc aggatgcaga
     2701 agacgaccgc cgccagggtg ccggagaggc gctcgcgctg cggcaggtcg agggtctcgc
     2761 cgatgaagtc cttgttgctc accgccgcca gcaggggatg gccgaggtcc gcgatctcgt
     2821 gcaggcgccg ggtcacctcc agcgtgtgca gcgtgttctt gttgaggtcg tggccgggat
     2881 cgatgatgat ctgctccggg cgtacgcccc ggtcgagcgc gagctgcacc cgccgccgca
     2941 ggaagtccgc gacctcggcg gtcacgtccc cgtacgtggg gcccgggtac ggcgtccgcg
     3001 gcgcggccag gctgtgcgtg atcaccaggg cggcgtccgt gccggccacc acgtccgcca
     3061 tcgcgggatc gcgcaggccc gacgtgtcgt tcacgacccc ggccccggcc ttgacgacct
     3121 cgcgggcgac ctccggccgg aaggtgtcga ccgagaccac cgcgagcccc tgcgccgcct
     3181 cgaccaccgg cagcacccgg tccagctcct cgctcgccga cacctcgggc cccggcgcga
     3241 acggcacgcc gccgatgtcg atccagtccg cacccgcctc ggcggccgcc cgcaccgcct
     3301 cggtggcccg gtccagggcg aaggtgcggc cccggtcgtg gaacgagtcc ggcgtgcggt
     3361 tcacgatcgc catcaccgcg acccggcggg agaagtcgta ccggcggccg ccgatgaccc
     3421 gttcggcgct gatcaacggg ggtgcgtagt cactcacgcc cgggagagta gcccggatcc
     3481 cgggcccgca ggacgggatc ggcggtgacg gtgatcatga tcaggggcgc cggcccggcg
     3541 cccggccgca ggagattccg gcgaatcccg aagggtcggg gcgggtccgg cgcgaagatg
     3601 gctacgtgtg ggcgtggctg cggggccggg tgctggcgcc ggtggacatc gggttcatcg
     3661 ccgaccgctc ggccgtcgcg tacgagggtg cggcgctggc cggcgagccg gccgtggtgg
     3721 cggcgctcgc ccgggcgccg gaggtgttcc tcgccgtcgc cgacgacccc gggaacgcgg
     3781 agatggccgt cgaccgcgag gatctccccg gcctgctgga ggtggtcacc gccgacgggc
     3841 gggcgtggga gctcctgatc gactgcaccg tcaagggcat cgtcgacacc ggcggctaca
     3901 ccggcgtcga ggacgacctc gtggagctgg cgctcgcgcg cgggcccggc gtcatctcca
     3961 ccgcgcacct cgagcgggag gtgtactggc tgcgcctgac ccggcccgag ccgcccgagc
     4021 gggtgctggc ccgcttcgtc ggcgccgtcg tcgacgcgca ccgggagctg gcccggcggc
     4081 tggacatcgc gctgcccggc tgaggcggca cccgctcggg tgccgcctca gcggcgggct
     4141 caggcgtagc tgtagttcag ggtgctgatg tcacgcaccg ccgagggcgt ccggccactg
     4201 gcgaacgggc gcatcgtctc ggtgttgtcg atgacgccgg aggtcccggt ccgcggctgg
     4261 taggtgtcga cgccgcggct ggtctgccac agccaccaga gccggtcgac attgcagtgg
     4321 tgcagccaga agaccgggtc gttgggtgcc tcgcgggtgc ccatgtgccc gcccacgtag
     4381 ttgtggaccc ggttgtgcag gtcgatctcg ttgcggttgc ggtagctgtt gctcacggtg
     4441 gagttccacg gccactggtc gtacaccgtg tagctctgca ccgtgctcac cgtgctggcg
     4501 ctgggacgcg tggcgttctg gccgagccgg cgctgcaggt aggaggtggc gatgctgctg
     4561 cggtagatgt tccagcccca cgccgggctg aagttgccgc tggacacgct cccgctggtg
     4621 atggcaccac agaagtccgt ggtgaacggc gtggcggtgc cggtccagtc ccagtacggg
     4681 atgaagatgt cggggttgcc gctgacggtc tgcaggtcga actccagcag ccgcaggtac
     4741 tcgcggtgcc acgggaagaa cgacggtgac tggtgggcgt ggctgatgtt gtccgaggtg
     4801 cgggcgaaga atgcgatgtg ctggtcgatg taccagttgt agttccgtcc attcgcggac
     4861 gtcgtcttga actggcgcag ggcgttgacg aacgcggtct tctcggtcga ggtcagagcg
     4921 gcgacactct tgcgggcgac tgccattgtt cggttacctt tcgaatggtg gggaagggca
     4981 ggggcgcggg accaccgtgg tcagtggtgg tgcgccagcc ccgcctcgtc gccgagctcg
     5041 aactcgccca gggtgtcgac cgccttcttg gccagcttct tgaggccgtc cttgtgctgg
     5101 ctctcgtcga tctccaccgg gtcgtagtgg ttgagggcgc tgatgtagcc gacccccacg
     5161 tgcggtgccg acccgtcctc cggcacgaac agcgtgtcga tcggggtgag cgccagcttc
     5221 ttgttgttga tgtgcagctc gtgcttgccg ttcgagtggt gtttaccctt gatcttcttg
     5281 cccttgtaga cctcgtcgaa gtcgcgcggg tccgcggcgg cgcgctgggg ggccgcgtcg
     5341 gagcgggcgg cggtgacggt ctgcgtggcc accgcgacgg tggtggcgct caccgcggcg
     5401 gcggcgccgt agcgcaggac gtcacgtcgg ctcatgtcgg ccataggaat cctcttctgg
     5461 aagaaagcca ttcggtacgg gcagctcgtg gttgccgttt ccatcgacat cgtcgactgg
     5521 aggaaaacta ctggcgcgag ccgcgcgctc gcaattggac ggtcgtggcg gatgacgagc
     5581 ctttcagcca ttggggatcg acattcgtca ctgataggtg gacgatggac tacccgtgcc
     5641 tggacgggaa acaggcaaga atccgaggag tttccatgcg agcaatcccc atcctggcgg
     5701 ccgtgctggc cgccggcgcc ggcctgatcg ccaccgccgg ccccgccgtc gccgcccccg
     5761 cgccgcaacc ggccgcgcag gccggcgccg acctggccgg attcttcgcg cagcgctacg
     5821 ggggacacca catcatgggc tggggccgcg acgagtcggc ctgctcctac atcgacggcg
     5881 tgcagctcgt gctctacccc ctcggccgca accggtacgt gagtgcgctg caggcctacg
     5941 aggaggagcg cggcgtcaag aacatcacca aggcctcggc ggacgtcctc ggcgaggccc
     6001 gcctcgtccc gcccgcggac cccgtcgcgc actgccccgt cttcgtcacc gctgcccccg
     6061 aggggagcta gccacccggg tcccctcgcg ggggcgcagg cactcggcgg ccgtcctgac
     6121 gagggcggcc gtcgactgct tttgcgccac cgcttccacc ggcgacagtc tcttgtgtgc
     6181 gatacgcagg gtgatttcgg gcgcgcccgg gccgccgccg cgggcgggcg cgcgcgaacc
     6241 ggggcgaata cccggggaca ggcaatctat gcaggggttt ttccattaat tgtgcgaggg
     6301 cgatcaggtt cgccgacccc gcttggcggg tagcggaagg ggcgaagcac ccacggacga
     6361 accatcatgg aggtagcgat gagtgacccc tacacgggcg cccgcgcacc ggacgccacc
     6421 ccgggctacg gcgaggtgcc ggtccagccg gtggtcgagg cgtaccccgc cacgacgggc
     6481 cccgccaccg ggaccggcct gaccaccggc gacgaccgtg ccttcggcgc gtcctcgtcc
     6541 ggcgggggca ccgcggaagg cgccaaggag caggccaagc acgtcgccgg agagggcgcg
     6601 cacgccgccg ggcaggccgc cggcgagatc aaggacaccg ccgtggagca ggcccagcgg
     6661 gtcggcgccg aggcgaagac gcagatgcgc aacgtcgcct ccgacgtccg ggaccgggtc
     6721 agcacccagg cccgggacca gaacgacaag ctcgtcggca ccctgcgaca ggccgccgac
     6781 cagctcgacg agatgcgcgg cgagcggcag gactcgccgg ccgcgcaggt cgtctcccgc
     6841 atcgccgagg gcggccggca gatggccgac tacctggacc gcaacggtcc cgagggcgtc
     6901 ctgcgcgagg tcaccgactt cgcccgccgc cgtcccggcg cgttcctcgc cacggcgctg
     6961 gccgccggtt tcgtggccgg acggctcggc aagagcgtca tgaaggcgga cgacaccgcc
     7021 ggcaagccgc ggaccgacac cttcgtctcg ggtgcggacg ccggctacgg ctccggcttc
     7081 gcggacacga cggcgggtta cccggccgcc ggcacgacca ccggataccc ggccgcgggc
     7141 gcgaccgcgg gctacccgag tgccggcacg accgccggct acccggccgc cggcacgacg
     7201 gccgcgggca ccgagtacgc ctccaccggc acgggcacgc ccgccgtcgt ggacgagcag
     7261 tatccgggcg gcacccgatg accgccccgt acccgaccgg ccacgaccgg gccgaggagg
     7321 tctccgcgac ctccgtgggc gagctcatcg gcaacatcag caacgacctg tcccagctgt
     7381 tccgccagga ggtggagctg gccaaggccg agctcaagca ggaggccggc aaggccggca
     7441 aggcggcggg catgctcggc ggcgccgggt tcgccggcta cctcgcggtg gtgctgctga
     7501 gcctggccct ggtcttcggg ctgggcaacg tcatggacct gggctgggcc gcgctcatcg
     7561 tcgcggtgat ctggggcgcc gccggcgccg tcctctacgc ggccggccgc aagcggctga
     7621 agtccgtgga ccccgtgccc cgccgcaccg tcgacaccat caaggaggac gcgcaatggc
     7681 tgaagaaccc gaccggctga ggcaggagat cgagcagacg cgggcctcgc tgacgcgcga
     7741 cgtggacctg ctggccgaga agacgagccc caagcaggtg gcccggcggc gctggaccgc
     7801 ggtgaaggag aaggtcatgg gtacgaccga gcacgcccgc cacagctcgt ccggggccgc
     7861 gcacgccgcg gcgagcacgg tgcaggacaa ggcgtcgcgg ctgggtgaca aggcgtccga
     7921 gctgagcgac cgggccggcg agaaggcgca cgacgtcgcc gacgcggtca agggcgcgcc
     7981 gcaggcgatg acgcagcagg cccagggcaa cccgctcgcg gccgggatca tcgccttcgg
     8041 cgtgggcatg ctggcggcca cgctgatccc ggtcaccgag gccgagaagc gcgccggcga
     8101 gcagctcaag gagcacggcg gcgaggtgac cgaccgggtc aaggacgtcg ccatggagct
     8161 caaggacgag gtcaccggca cggtgcagca ggccgcgggc cagctcaagg agacggcccg
     8221 cgacgccgcg gagaccacca aggaccaggc ccgttcctcg gcgcaggacg ccaaggagca
     8281 gagcaagcag gccgtcgcgg acgcccgttc ctgaggtgct tccgccacgt ttgccgcttc
     8341 ctgccggggg caaccggcag gaagcggcaa acgagggagc gcccatgagg atcgggtaca
     8401 agctggcgtc ggagggattc gggcccaagg agctgatcgc gcaggcggtc gccgccgagc
     8461 gcgccgggtt cgacttcgtc gagatcagcg accactacca cccctggctg gacgcccagg
     8521 ggcattcgcc gttcgcctgg acggtgctcg gggccatcgc ggcgcgcacc gaccggatcg
     8581 gcctggccac cggcgtgacc tgcccgtcgg tgcgctacca cccggcgatc atcgcgcagg
     8641 ccgccgccac cctcgcgatc acctcggacc accgcttcac cctgggcgtc ggctcggggg
     8701 agcggctcaa cgagcacgtc gtcggacagg gcttcggcaa cgtgcgcgag cgccaggagc
     8761 ggctgcgcga ggcgctggag atcatcaagc tgctgtggcg gggtggctac cagtcgtacg
     8821 agggcaagca cctgcggctc gaggacgccc gcgtcttcga cctgcccgac gagcctccgg
     8881 tgatcgcggt ggccgccggc ggtcgcgacg ccgcgacgat ggcagccgag ctgggcgacg
     8941 gcctgttcgc caccgagccg cgcgccgacc tggtcgagac ctaccgcggg gccggcggcg
     9001 ccggaccccg ctacgccgag gtgccggtgg cctgggcccc caccgccgag gagggcatcc
     9061 gggaggcgca ccggaccacc cgctgggcgc tgaccggctg gaaggtgatg agcgagctgc
     9121 cgaacccggt caacttcgag gcggcgtcgc agaccgtccg cgaggaggac gtccgggaga
     9181 agttcgccgc cgggccggac ccggaaccgt acctcgaggc cgtccggacg tacgtggcgg
     9241 cgggcttcga ccacatcgtg ctgcagaacg cggggccgga cccggacggc ttcatcgact
     9301 tcttccagaa ggaactgggc gaccggctgc gcagcgcgtg accgcgcgac ggtcactcga
     9361 agtggtggct tcgtcacggg caatcggggt acccaccgcg gccatgacga tgatccagac
     9421 cggccgggcc gccgcgccgg tgccgcccga gaccgccgac gtcaccgtgg tggtcgccac
     9481 ccgcaaccgg cgcgaccgcc tccgggagac ccttccccgg cacgcggccc cggtcgtcct
     9541 cgtggacaac ggctccgacg acggcggccc ggacgccgtc gccggcgtcg acgtcgtccg
     9601 cctcggcgag aaccggggcg cggccgcgcg caccgtcggc gtcgagcgcg ccgccacgaa
     9661 gtacgtggcc ttcgccgacg acgactcctg gtgggagccg ggctcgctgg cccgggcggc
     9721 cgcgctgctg gacgcgtacc cccggaccgc cctgatctcg gcccgggtgc tggtcgggcg
     9781 gcagcggcgc gaggacccca tctcggcggc catggccgcc gcgccgctcg gcgtgcgcga
     9841 cggcgcaccc ggccccgaga tcctcggctt cctcgcctgc gccgtcgtgc tgcggcgcga
     9901 cgcctacctg cgggccggcg gcttccatcc ggcgctgcac gtgtacggcg aggaggcgct
     9961 gctcgccatg gacctcgccg ccgcgggcca ccggctggcg tacgcgccgt cgctgaccgt
    10021 gcggcacatg ccgctgccgg cgggccggga caccggcctg cggcaccgcc gcgaggtgcg
    10081 caaccgggtg ctgaccgcgc tgctgcgccg cccgcccgcg gtggtgggac ggaccgtcgc
    10141 ggccgcctgg cgcgcggacc gggcgggaac ggtcgacgcc ctcaggatgc tgccccggat
    10201 cctgggacag cggcgcacgc tgccccagga cgtcgagcgg gatctgcgcg tcctcggctg
    10261 ctgagccggg tcaccagccg gacggcggcg cctcgatgtg caccgccttc gtcacggtca
    10321 gctcgtcgag cagctccggg ccgtacccga agccctgccc gctgccgcgc cgcgggtgcg
    10381 cggcaccgcc cggggcgccg ccgaagaccg cgttcacctt caccgtgccg acgcc
//
