LOCUS       NZ_BMQY01000002        10816 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Couchioplanes caeruleus subsp. azureus strain JCM 3246 sequence02,
            whole genome shotgun sequence.
ACCESSION   NZ_BMQY01000002
VERSION     NZ_BMQY01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Couchioplanes caeruleus subsp. azureus
  ORGANISM  Couchioplanes caeruleus subsp. azureus
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Couchioplanes.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 909631)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 64X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014648355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:32:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,801
            CDSs (total)                      :: 6,731
            Genes (coding)                    :: 6,630
            CDSs (with protein)               :: 6,630
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 101
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 101
            Pseudo Genes (frameshifted)       :: 20 of 101
            Pseudo Genes (incomplete)         :: 93 of 101
            Pseudo Genes (internal stop)      :: 0 of 101
            Pseudo Genes (multiple problems)  :: 12 of 101
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:23:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 592591
            Orig. end                         :: 603407
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMQY01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1237..1482
                     /locus_tag="IEY99_RS09735"
                     /old_locus_tag="GCM10010166_19550"
     CDS             1237..1482
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287422.1"
                     /locus_tag="IEY99_RS09735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19550"
                     /product="DUF2630 family protein"
                     /protein_id="WP_189201565.1"
                     /transl_table=11
                     /translation="MDDKSVLDRIHGLVDEEHKLREQLRRGEISGEEERTRLQEVEESL
                     DQCWDLLRRRRAAREAGNDPDAERAHSTGEVESYLQ"
     misc_feature    1256..1269
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(1556..2473)
                     /locus_tag="IEY99_RS09740"
                     /old_locus_tag="GCM10010166_19560"
     CDS             complement(1556..2473)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583044.1"
                     /locus_tag="IEY99_RS09740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19560"
                     /product="serine protein kinase RIO"
                     /protein_id="WP_373293309.1"
                     /transl_table=11
                     /translation="MREHDSSGPSTMRRGRRKFDDDDSDFVRRGRLEPRLAEDPDLPET
                     GDRWSTWDGALHGPAPRPAWVRTEHGAVDTELGVLKTGKEADVHLVRRAVPGTGEVSML
                     AAKRYRDGEHRLFHRDAGYLEGRRVRRSREMRAMTNRTAFGKELIAGQWAAAEFGALSR
                     LWQAGQEGGTISVPYPVQLIGTELMLEFIGDWETGEAAPRLAQVRPDRDGLEDLWGQMV
                     DALSVLARAQLAHGDLSPYNTLVHDGRLVLIDLPQVVDVIANPQGGEFLARDVRNVAAW
                     FTSRGLPVDVDEVIERLCYEAGLR"
     misc_feature    complement(2503..2517)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.03"
                     /tool="antismash"
     gene            complement(2626..3276)
                     /locus_tag="IEY99_RS09745"
                     /old_locus_tag="GCM10010166_19570"
     CDS             complement(2626..3276)
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014441765.1"
                     /locus_tag="IEY99_RS09745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19570"
                     /product="HAD family hydrolase"
                     /protein_id="WP_189201567.1"
                     /sec_met_domain="HAD_2 (E-value: 1.2e-13, bitscore: 47.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLVFLALDDTLLDRSGAFKLWAKGFLDEIAAPQEDLDWLLSVDA
                     DGLTSRWDLADLIRDRYQLDIPSIDLLEELHEGPLAYERLDPLVACALQIAGDAGWVPV
                     VVTNGPHEQQESRIRRTGLDRYIADWVISEQAGVSKPNPRIFALAAQRVRMRLSGAWIC
                     GDSPEADIGGAAAMGLPSVWLHRGREWIDNRFAPTRVVGNVIQGLSAIMATTK"
     misc_feature    complement(3340..3356)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.64"
                     /tool="antismash"
     gene            3438..4013
                     /locus_tag="IEY99_RS09750"
                     /old_locus_tag="GCM10010166_19580"
     CDS             3438..4013
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513137.1"
                     /locus_tag="IEY99_RS09750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19580"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_189201569.1"
                     /transl_table=11
                     /translation="MPRVSQDQLEARRHEILAAARGCFARYGYEGATVRRLEEATGLSR
                     GAIFHHFRDKDSLFLAVAEDDAAAMVETVARNGLVQVMRDLLERAGASGDTAGWLGTQL
                     EVSRRLRTDPEFAQRWSERAAAVEAATRERLQRQRDARVLRDDVPVDVLTQFLELAYDG
                     LVLHLATGRPPGELGRVLDVVEEAVRRP"
     gene            4075..4998
                     /locus_tag="IEY99_RS09755"
                     /old_locus_tag="GCM10010166_19590"
     CDS             4075..4998
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018802925.1"
                     /locus_tag="IEY99_RS09755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19590"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_189201571.1"
                     /transl_table=11
                     /translation="MALAAQGDTWGISGPAFLLLYVAAMVAVAVIAVVHRRLLFSGPRD
                     ASVDTLGPQQVAYLNGGDKLAVYASLGGLRGAGAIDSSAGSKLKQSGPMPAGVTPLDTA
                     VYNAAGRRIPVRQVLADSWVRSALDQLRAGLEANGLAVSAAQTRAARSWLFAGLALIAL
                     GAARLAAGIANDKPVGFLVVALIPAVVLTIFLGRANRRATAVARKGMADLRRRYDHLAP
                     HQSPSYATYGAAGAAMGVALFGAASLYTMDPAFAAEAEIQRLNATGSPSTWTSTSSCGS
                     SSSSCSSGSSSCGGGGGCGGGGGCGG"
     gene            5001..5816
                     /locus_tag="IEY99_RS09760"
                     /old_locus_tag="GCM10010166_19600"
     CDS             5001..5816
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722901.1"
                     /locus_tag="IEY99_RS09760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19600"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_373293326.1"
                     /sec_met_domain="DUF692 (E-value: 3.8e-79, bitscore: 261.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAYGVGIGWRPEISGYVAALPGLRFTEVVAESLHAHAELPRGLA
                     ELRARGVSVVPHGVKLSLGGAEPVDPARVGHLAAVARRLDAPLVSEHIAFVRAGGVEAG
                     HLLPVPRSREAVDAVVANVRRTREELDVPLALEPIAALFDWPDDELDEGAFLTEILERT
                     DALLLLDVANVYANARNRGTDPAALLDRLPLDRIAYCHVAGGSAHDGVYHDTHTDAVPR
                     EVLDLVAELCRRHRPPALMLERDGAYPPAAELSAELDAIAAASGYPVVT"
     gene            5813..6250
                     /locus_tag="IEY99_RS09765"
                     /old_locus_tag="GCM10010166_19610"
     CDS             5813..6250
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018722900.1"
                     /locus_tag="IEY99_RS09765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19610"
                     /product="hypothetical protein"
                     /protein_id="WP_189201575.1"
                     /transl_table=11
                     /translation="MTSLAERQAALVAALTSGAPVPPGFDARLVGIARVALLRKRAGEV
                     ARQWPELAAALGPRWPDVWAGWAAHRPTQGSLRDGWDLARDLAGRGDLPPAAAAELATR
                     EAAMRYDGRSAPQTRRLPALRRAGGCLALQAGGRVRVLRRP"
     gene            complement(6236..7615)
                     /locus_tag="IEY99_RS09770"
                     /old_locus_tag="GCM10010166_19620"
     CDS             complement(6236..7615)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEY99_RS09770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010166_19620"
                     /product="hypothetical protein"
                     /protein_id="WP_189201577.1"
                     /transl_table=11
                     /translation="MLDDDGVDQAALTGTDPEARRAALGELTARCQAARSAPARLVPLL
                     EPLVHDPRSRDDAVVALRRCGRAAARYAGFLGEAAAAAPEPATRRRAVETLMLIGDPRW
                     VDPVVCEGAALLPHALMPLPCPPEVRDTVRRRLATLIARAESGRGEAAGESAGEAAGGA
                     GALATLCEIVARWGTDARETVPELLDALPHAPGAATRALLAAGHLGAPMVPGLRAAASR
                     PGGLPAATALFRLTGDPTPALRALLTARPPVPPGVVALLGPLALPLVPEFELLLTGEAA
                     AAPPQCAAQVLAAQVVIAATGDTTAALPTLHAVLTAGGHRAVLAAAVVADLAAAGPGAS
                     GGGWRGEMAVWVPELRALLDDPWAGVAAAGALWRLGAAAGELVPVAARAVEEGRRPAAA
                     VALLHELGATAEAARLAARDERLTVGGADDDIVVNDELLRDRLLATARATATGSGGSGA
                     A"
     gene            7703..8458
                     /locus_tag="IEY99_RS09775"
                     /old_locus_tag="GCM10010166_19630"
     CDS             7703..8458
                     /NRPS_PKS="Domain: PKS_KR (8-148). E-value: 1.2e-08. Score:
                     27.0. Matches aSDomain:
                     nrpspksdomains_IEY99_RS09775_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018817324.1"
                     /locus_tag="IEY99_RS09775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19630"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_189201579.1"
                     /transl_table=11
                     /translation="MDLGLADRVYVLTGASRGLGFATAQALVADGARVVISSRSPAGVE
                     AATAALGDAAAGVAADLADPGTPELLLRTATERFGRCDGALISVGGPAQGTAASRTDDE
                     WRAAFETVFLGAVRAARTFAATLPAGGAIGFVLSTSARTPIPGLGLSNGLRPGLAGVAK
                     DMADEYGPRGVRVLSLLPGRVLTDRNRELFAATGDPERAAAEAAAAVPLGRLGEPEEFG
                     RVAAFLLSPAASYLTGISVPVDGGALRTL"
     CDS_motif       7727..7822
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IEY99_RS09775_0001"
                     /evalue="1.30E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="IEY99_RS09775"
                     /protein_end="40"
                     /protein_start="8"
                     /score="27.5"
                     /tool="antismash"
                     /translation="VYVLTGASRGLGFATAQALVADGARVVISSRS"
     aSDomain        7727..8146
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY99_RS09775_PKS_KR.1"
                     /evalue="1.20E-08"
                     /label="IEY99_RS09775_PKS_KR.1"
                     /locus_tag="IEY99_RS09775"
                     /protein_end="148"
                     /protein_start="8"
                     /score="27.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VYVLTGASRGLGFATAQALVADGARVVISSRSPAGVEAATAALGD
                     AAAGVAADLADPGTPELLLRTATERFGRCDGALISVGGPAQGTAASRTDDEWRAAFETV
                     FLGAVRAARTFAATLPAGGAIGFVLSTSARTPIPGL"
     gene            complement(8565..9203)
                     /locus_tag="IEY99_RS09780"
                     /old_locus_tag="GCM10010166_19640"
     CDS             complement(8565..9203)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474098.1"
                     /locus_tag="IEY99_RS09780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19640"
                     /product="M15 family metallopeptidase"
                     /protein_id="WP_189202170.1"
                     /transl_table=11
                     /translation="MILLSDPRVAAVPSEDGGEPMIDVRDVPELRLDGRAADGEGSYAR
                     LRAEVASRLVDAQRKLPEGLRLLIVEGYRPYATQHAIWTRYRDELRRLHPSWPDGRLLV
                     ETSKFVSPVEVAPHSTGGAVDLTLCTADGEELDMGTPIDATPVECDNACFTGARNIPAA
                     AVENRRLLSDALSAVGMVNYPTEWWHWSYGDRYWALLTGASHAPYGPVH"
     gene            complement(9394..10419)
                     /locus_tag="IEY99_RS09785"
                     /old_locus_tag="GCM10010166_19650"
     CDS             complement(9394..10419)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012512.5"
                     /locus_tag="IEY99_RS09785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010166_19650"
                     /product="threonine/serine dehydratase"
                     /protein_id="WP_189201581.1"
                     /sec_met_domain="PALP (E-value: 8.7e-72, bitscore: 237.5,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGMAVTRRLARSDVEAAARFLDGRVVRTPVVRSAGLDARAGTRLL
                     FKAENLQDGGSYKMRGATVAVGRLAAAGGATGVVCQSTGNHALAVALAARRHGLAAVVV
                     LPPDAPATKVRQAEAAGARVVVGGAGAEERLRLVEGIATETGYAVIDAYDHPDVVAGQG
                     TAALELIEEAERAGTPLDALVVPVGGGGGAAGASLAATGRRIDLYGVEPVGCDSLARSL
                     EAGRRVPVVPAPTLADGLRPTCVGALPFGILHEAGIGAVRVGDDAIAEAFRLVMSELRL
                     LAEPSGAAALAGALRVAERGIHRTVGVLLTGGNVAPALVADLLTTGRDLTRARRAHPRW
                     A"
     misc_feature    complement(10658..10674)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.2"
                     /tool="antismash"
ORIGIN
        1 gatctccgcg gcgatcaacg aggccgacct caccgcggtc agcgtgctct ccggcaaccg
       61 caacttcgag ggccggatca acccggacgt caagatgaac tacctggcgt ccccgccgct
      121 ggtcgtcgcg tacgccctgg ccggctcgat ggacatcgac atcaccaccg agccgctggg
      181 catcggctcg gacggcaagc cggtcttcct gcacgagatc tggccgagcg ccaaggagat
      241 cgacgacgtc atcgccaccg cgatcggcgc cgagggcttc agcacggcgt accaggacgt
      301 cttcgccggc gacgagcagt ggcagtcgct ccccaccccg accggcaaga cgttcgagtg
      361 gtccgaggac tccacctacg tccgcaagcc cccgtacttc gagggcatgg ccgccgaccc
      421 gagcccggtg aacgacatct ccggcgcgcg ggtgctggcc aagctcggcg actcggtgac
      481 caccgaccac atctcgccgg ccagctcgat caagccggac tcccccgcgg gcaagtacct
      541 cgcggagcac ggggtgcccc ggcacgagtt caactcgtac ggctcccgcc gtggcaacca
      601 cgaggtgatg atccgcggca ccttcgccaa catccggctg cgcaaccagc tggtgccggg
      661 cgtcgagggc ggcttcacgg tcaaccacct gaccggcgag cagaccacca tctacgacgc
      721 gtcggtggcc tacaaggagg ccggtgtccc gctggtcatc ctggccggca aggagtacgg
      781 ctcgggctcg tcgcgcgact gggcggccaa gggcacgatg ctgctgggcg tccgcgccgt
      841 catcgccgag agctacgagc gcatccaccg ctccaacctg atcggcatgg gcgtgctgcc
      901 cctgcagttc ccgcagggcc agaacgccga gtcgctcggc ctcaccggca ccgagacctt
      961 caccttcacc ggcgtcaccg agctcaacga gggccgcacg ccgcgcacgg tcaaggtcac
     1021 cacggacacc ggcgtggagt tcgacgccgt cgtccgcatc gacacccccg gcgaggcgga
     1081 ctactaccgc cacggcggca tcctgcagta cgtcctgcgc aagatgctga ccagctgacg
     1141 cgtacgctcg cggaaacgcc ccgccgtccc ggatggacgg cggggcgttt tccgtcggcg
     1201 tacccgcggc ctcgtcacag gtcgtaagct cacgccatgg atgacaagtc ggtgctggac
     1261 cgcatccacg gcctggtgga cgaggagcac aagctgcgcg agcagctccg ccgcggcgag
     1321 atcagcggcg aggaggagcg cacccggctc caggaggtcg aggagtccct cgaccagtgc
     1381 tgggacctgc tgcgccggcg gcgggccgcc cgggaggccg gaaacgaccc ggacgccgag
     1441 cgggcccaca gcaccggcga ggtggagagc tacctccagt gacgcggcac cggccgcggc
     1501 cgccgggtgg ccggcggccg ggggccggat cgcgcccggc gcgatgccgc gcggctcatc
     1561 gcaggcccgc ctcgtagcag agccgctcga tcacctcgtc gacgtccacg ggcaggcccc
     1621 gcgaggtgaa ccaggccgcc acgttgcgca cgtcgcgggc gaggaactcg ccgccctgcg
     1681 ggttggcgat cacgtcgacc acctggggca ggtcgatgag caccaaccgg ccgtcatgga
     1741 ccagcgtgtt gtacggcgac aggtccccgt gggccagctg cgcacgggcg agcacgctga
     1801 gcgcgtccac catctggccc cacaggtcct ccagaccgtc gcggtccggg cgcacctggg
     1861 ccaggcgcgg tgccgcctcg ccggtctccc agtcgccgat gaactcgagc atcagctcgg
     1921 tgccgatcag ctgcaccggg tacggcaccg agatcgtgcc gccctcctga ccggcctgcc
     1981 agaggcggga cagcgccccg aactcggcgg ccgcccactg gccggcgatc agttccttgc
     2041 cgaaggccgt acggttggtc atcgcccgca tctcgcggga ccggcgcacc cggcgccctt
     2101 cgagatagcc ggcgtcgcgg tggaacaggc ggtgctcgcc gtcgcggtag cgcttggccg
     2161 cgagcatgct gacctcgccg gtgccgggga ccgcccggcg gaccaggtgg acgtcggctt
     2221 ccttgccggt cttgagcacg cccagctcgg tgtccacggc gccgtgctcg gtgcggaccc
     2281 aggcgggtcg cggtgcgggg ccgtgcaggg cgccgtccca ggtcgaccag cggtcgccgg
     2341 tctcgggcag atcggggtcc tcggcgagcc gcggctcgag ccggccgcgc cttacgaagt
     2401 cggagtcgtc gtcgtcgaac ttgcggcggc cgcggcgcat ggtggacggg ccggaagagt
     2461 cgtgctcgcg cactggaggg tctcctcgga gaaggaagaa ggaagtttcg gaacctgggc
     2521 acggcgaagg acaacgacag ccatgaggtc cacccccttc tcttccgagg aatctcgcgg
     2581 aacacgtacg ggagcactct gtcgggtgat cccgggtcac gcaacctatt tagtggtcgc
     2641 catgatggca gacagtccct ggatgacgtt gcccaccacg cgggtcggcg cgaagcggtt
     2701 gtcgatccac tcgcgtcccc ggtgcaacca cacgctgggt agtcccatcg cggccgcccc
     2761 gccgatgtcg gcctccgggc tgtcgccgca gatccaggcc ccgctcagcc gcatccggac
     2821 ccgctgggcg gcgagcgcga agatgcgcgg gttgggcttg ctcaccccgg cctgctcgga
     2881 gatgacccag tcggcgatgt agcggtccag accggtgcgc cggatccggg attcctgctg
     2941 ctcgtggggg ccgttggtga ccacgaccgg cacccagccg gcgtcgccgg cgatctgcag
     3001 cgcgcaggcg accagagggt cgaggcgctc gtaggcgagc ggcccctcgt gcagctcctc
     3061 cagcaggtcg atggagggga tgtcgagctg gtagcggtcg cggatcaggt cggcgaggtc
     3121 ccaccgcgag gtgagcccgt cggcatccac ggacagtagc cagtcgagat cctcctgtgg
     3181 tgccgcgatc tcgtcgagga agcccttcgc ccacaacttg aacgcaccgc tgcgatcgag
     3241 cagggtgtcg tccagggcga ggaagacgag aggcacccgg gcactgtacg tgagtgaata
     3301 cggcagcgaa cagcccacga gtgctatcca gggtccgtct gccgggtctt tttgcaccat
     3361 caggaggctg aggctccggc tgccaagccg tacgtacgga ttgcaagcgc gccgggcggc
     3421 gtgacaccat cgcttccgtg cccagggtaa gccaggacca gctagaggcc cgccgccacg
     3481 aaatcctcgc cgcggcccgc ggatgcttcg cgcggtacgg ctacgagggc gccaccgtgc
     3541 gccgcctcga ggaggccacg ggcctgtccc ggggcgcgat cttccaccat ttccgggaca
     3601 aggactcgct gttcctcgcg gtcgccgagg acgacgccgc cgcgatggtc gagaccgtcg
     3661 cccgcaacgg tctcgtccag gtgatgcgcg acctgctgga gcgggccggg gccagcggcg
     3721 acaccgcggg ctggctcggc acccagctgg aggtctcccg gcggctgcgc accgaccccg
     3781 agttcgccca gcgctggagc gagcgggccg cggcggtcga ggccgccacc cgggagcgcc
     3841 tgcagcgcca gcgcgacgcc cgcgtgctgc gcgacgacgt gccggtcgac gtgctgacgc
     3901 agttcctcga gctggcgtac gacgggctcg tgctgcacct ggccaccggc cgcccgccgg
     3961 gcgagctggg ccgggtcctc gacgtcgtgg aggaagccgt caggcggccg tgacctgaat
     4021 gttgcactga tctgcacaat gagggccagc aaccctgcga acacgggagg tcacatggcc
     4081 ctcgcagcac aaggcgacac ctggggcatc tccggcccgg ccttcctcct gctctacgtc
     4141 gccgccatgg tcgcggtggc ggtcatcgcg gtcgtccacc gccgcctcct gttctccggt
     4201 ccccgcgacg cctccgtcga cacgctcggt ccgcagcagg tggcatacct gaacggcggc
     4261 gacaagctcg ccgtctacgc atccctcggc gggctgcgcg gtgcgggagc catcgacagc
     4321 tcggcgggca gcaaactgaa gcagagcggt cccatgcccg ccggcgtcac cccgctcgac
     4381 accgccgtct acaacgccgc gggccggcgg atcccggtcc gtcaggtcct cgccgactcg
     4441 tgggtgcgct cggcactcga ccagctgcgc gccggcctgg aggcgaacgg cctcgccgtc
     4501 agcgcggcac agacgcgcgc ggcccggtcc tggctcttcg ccggcctggc cctgatcgcc
     4561 ctgggcgccg cccggctggc cgccggaatc gccaacgaca agccggtcgg cttcctcgtc
     4621 gtcgccctga tccccgccgt cgtcctcacg atcttcctgg gccgggccaa ccgccgggcc
     4681 accgcggtgg cgcgcaaggg gatggccgac ctgcggcgcc ggtacgacca cctggcgccc
     4741 caccagtcgc cgtcctacgc gacctacggc gcggcgggtg cggcgatggg cgtggccctg
     4801 ttcggcgcgg cgtcgctcta caccatggac ccggccttcg cggcggaggc ggagatccag
     4861 cgcctcaacg ccaccggcag ccccagcacc tggacctcca ccagctcctg cggcagcagc
     4921 agctcctcct gcagcagcgg cagcagctcc tgcggtggcg gcggcgggtg cggaggcggc
     4981 ggcgggtgcg gcgggtgaac ctgcccgcgt acggcgtcgg catcggctgg cgccccgaga
     5041 tctccggcta cgtggccgcg ctgcccgggc tgcgcttcac cgaggtcgtc gccgagtcgc
     5101 tgcacgcgca cgccgaactg ccccgaggcc tcgccgagct gcgcgcacgc ggcgtctcgg
     5161 tggtgccgca cggcgtcaag ctctccctcg gcggcgcgga gccggtcgac cccgcccggg
     5221 tcggccacct ggcggcggtc gcccggcgcc tcgatgcccc gctggtcagc gagcacatcg
     5281 cgttcgtacg ggcgggcggg gtggaggccg ggcacctgct ccccgtgccc cgcagccggg
     5341 aggccgtcga cgcggtggtc gccaacgtgc ggcgtacccg cgaggagctg gacgtgccgc
     5401 tcgccctcga accgatcgcc gccctgttcg actggccgga cgacgagctc gacgagggtg
     5461 cgttcctgac cgagatcctc gagcgtacgg acgccctgct gctgctcgac gtggctaacg
     5521 tgtacgccaa cgcgcgtaac cgcggcaccg acccggccgc cctgctcgac cggctgccgc
     5581 tcgaccggat cgcctactgc cacgtggccg gcggctcggc gcacgacggc gtctaccacg
     5641 acacgcacac cgacgcggtg ccgcgggagg tgctcgacct cgtcgcggag ctctgccgac
     5701 ggcaccggcc cccggcgctg atgctggaac gcgacggcgc ctacccgccc gccgccgagc
     5761 tgtctgccga gctggacgcg atcgccgccg cgtccggata tccggtggtc acatgacgtc
     5821 cctggccgaa cggcaagcgg cgctcgtcgc ggcgctgacc tcgggcgcgc ccgtaccccc
     5881 gggtttcgac gcccggctgg tcgggatcgc ccgggtcgcg ctgctgcgca agcgggccgg
     5941 cgaggtcgca cggcagtggc ccgagctggc cgcggccctc ggcccgcggt ggccggacgt
     6001 gtgggccggg tgggccgcgc accgcccgac ccagggttcg ctgcgcgacg gctgggacct
     6061 ggcccgcgac ctcgccggcc ggggcgacct gcccccggcc gccgcggccg agctcgccac
     6121 ccgcgaggcg gcgatgcgct acgacggccg gtcggcgccg cagacccgcc gcctgcccgc
     6181 actgcgccgc gccggcggct gcctcgctct ccaggccggc ggccgggtac gcgtcctacg
     6241 ccgcccctga gccgccgctg cccgtcgccg tggcgcgggc cgtcgcgagc agccggtcgc
     6301 gcagcagctc gtcgttgacg acgatgtcgt cgtcggcgcc gccgacggtc agccgctcgt
     6361 cgcgcgcggc cagccgggcc gcctccgccg tcgcgcccag ctcgtgcagc agggcgaccg
     6421 ccgcggccgg gcgccggccc tcctcgaccg cccgcgccgc gaccggcacc agctcgccgg
     6481 ccgcggcgcc gagccgccac agcgcaccgg ccgccgccac accggcccac gggtcgtcca
     6541 gcagggctcg cagctccggc acccagacgg ccatctctcc ccgccatccg ccgccgctcg
     6601 ccccgggacc ggccgcggcc aggtcggcca ccacggcggc ggccagcacc gcgcggtgcc
     6661 caccggcggt gaggacggcg tgcagggtcg gcagcgccgc ggtggtgtcc cccgtcgcgg
     6721 cgatcaccac ctgggccgcg aggacctgcg cggcgcactg gggcggggcg gcggcggcct
     6781 ccccggtcag cagcagctcg aactcgggca ccagcggcag cgccagcggg ccgagcagcg
     6841 cgaccacgcc gggcggcacg ggcggccgcg cggtcagcag ggcccgcagg gcgggcgtgg
     6901 ggtcaccggt gagccggaac aacgcggtgg cggccggcag tcccccgggc cgggacgcgg
     6961 ccgcccgcag gccgggaacc atcggcgcac cgagatgacc cgcggcgagc agcgcccggg
     7021 tcgccgcccc cggcgcatgc ggcagggcat cgagcagttc cggcaccgtc tcccgggcgt
     7081 cggttcccca ccgggccacg atctcgcaca aggttgcgag ggcgcccgct cccccggcag
     7141 cctcgccggc gctttccccg gcagcctccc cgcggccact ctccgcgcgg gcgatgaggg
     7201 tggccaggcg gcggcggacg gtgtcacgga cctcgggcgg gcacggcagc ggcatcaggg
     7261 cgtgtggcag cagggcggcg ccctcgcaaa cgaccgggtc gacccagcgc gggtcaccga
     7321 tcagcatgag cgtctcgacc gcccggcgcc gcgtcgccgg ctccggcgcc gcggccgccg
     7381 cctcgccgag gaagccggca taccgcgccg cggcccggcc gcaccggcgc agggccacca
     7441 ccgcgtcgtc ccgcgagcgc ggatcgtgca ccaagggctc cagcaacggt acgagccgcg
     7501 cgggcgccga ccgggccgcc tggcagcgcg cggtcagctc cccgagggcg gcacggcggg
     7561 cctcggggtc cgtcccggtc aatgccgcct ggtccacccc gtcgtcgtcg agcaccgggg
     7621 caccctaccc gtcacgaccc gcatgtcgtc gcactcgtgc cgccgcggtg agcctccccg
     7681 gcggtgatgg caggatcggg gtatggatct gggcctcgcc gaccgcgtct acgtcctcac
     7741 cggagcctcg cggggcctcg gcttcgccac cgcgcaagcc ctcgtggccg acggcgcgcg
     7801 ggtcgtgatc tcctcccggt cgcccgccgg ggtcgaggcg gccaccgccg cgctcggcga
     7861 cgccgccgcc ggggtggccg ccgacctcgc cgatccgggc acgccggagc tgctgctgcg
     7921 caccgccacc gaacggttcg gccgctgcga cggcgcgctg atctcggtgg gcggcccggc
     7981 ccagggcacc gccgcctccc gcaccgacga cgagtggcgc gccgccttcg agacggtctt
     8041 cctcggcgcg gtgcgggccg cgcgcacctt cgcggcaacg ctgccggccg gaggcgcgat
     8101 cggcttcgtc ctgtccacgt cggcgcggac gccgatcccg ggcctgggcc tgtccaacgg
     8161 tctgcgcccc ggcctggccg gcgtcgccaa ggacatggcc gacgagtacg gcccccgcgg
     8221 cgtccgcgtc ctcagcctgc tccccggccg cgtactgacc gaccgcaacc gggaactgtt
     8281 cgcggccacc ggcgacccgg agcgcgcggc ggcggaggcg gccgcggccg tccccctcgg
     8341 ccggctcggc gagcccgagg agttcggccg ggtggcggcg ttcctgctct ccccggcggc
     8401 gagctacctg accggcatct cggtccccgt cgacggcggc gcgctgcgca cgctctgatc
     8461 cggccgatgg ccggcggcgc cgggccggag cgccgggcca gggcgcgaag gacgcgccgg
     8521 tgctcagtgc accggtccgt agggcgcgtg ggacgcgccg gtgctcagtg caccggtccg
     8581 tagggcgcgt gggacgcgcc ggtgagcagg gcccagtagc ggtcgccgta ggaccagtgc
     8641 caccactcgg tcggatagtt caccatgccc accgcggaga gcgcgtcgct cagcagccgc
     8701 cggttctcga ccgccgcggc cgggatgttg cgggcgccgg tgaagcaggc gttgtcgcac
     8761 tcgaccgggg tcgcgtcgat cggcgtaccc atgtcgagct cttcgccgtc cgccgtgcac
     8821 agggtgagat cgacggcgcc gccggtgctg tgcggggcca cctcgaccgg ggagacgaac
     8881 ttgctcgtct ccaccagcag ccgcccgtcc ggccaggacg ggtggagccg gcgcagctcg
     8941 tcgcggtacc gggtccagat ggcgtgctgc gtcgcgtacg gccgatatcc ctcgacgatc
     9001 agcaggcgca gcccctcggg cagtttgcgc tgggcgtcca ccagccgcga cgcgacctcg
     9061 gcgcgcaacc gggcgtagga gccctcgccg tcggcggcgc ggccgtcgag ccgcagctcc
     9121 ggcacgtcgc gcacgtcgat catcggctcg ccgccgtcct cgctgggcac cgcggcgacg
     9181 cgggggtcgg agagcaggat catcgcgcgc acgccgttcc cgcgaggcgt ccgggcgcgg
     9241 tcccgtcgtg cgccgtgcca cccggcatgg tgtcgagctc gttcacgtcc accgtcccgc
     9301 cgcctcaggc ccgcgaccag cgtcgtgcgg agcgtcggtc gggcgccgcg tttgctcctc
     9361 gaaccacccg tacggggtgg cacgccgcct cgttcatgcc caccgcggat gcgccctacg
     9421 cgcccgggtc aggtccctgc cggtggtcag caggtcggcg acgagcgcgg gtgcgacgtt
     9481 gccgccggtg agcaggacgc cgaccgtacg gtggatgccg cgctcggcca cccgcagcgc
     9541 gccggcgagc gcggccgcgc cggacggttc ggccaggagc cgcagctccg acatgaccag
     9601 ccggaacgcc tccgcgatgg cgtcgtcgcc gacccgtacc gcgccgatcc ccgcctcgtg
     9661 caggatgccg aacggcagcg cgccgacgca cgtgggccgc aggccgtcgg cgagggtcgg
     9721 ggccgggacg accggcaccc ggcgaccggc ctcgaggctg cgggccagcg agtcgcagcc
     9781 gaccggctcg acgccgtaca ggtcgatgcg ccggccggtc gccgccaaac tcgccccggc
     9841 ggcgcctccc ccgccgccga ccgggacgac gagggcgtcc agcggcgtgc cggcccgctc
     9901 cgcctcctcg atcagctcga gggcggccgt gccctgcccg gcgacgacgt ccggatggtc
     9961 gtacgcgtcg atcacggcgt acccggtctc ggtggcgatg ccctcgacca gccgcagccg
    10021 ctcctcggcg cccgcaccac ccaccacgac gcgcgcaccg gccgcctccg cctgccgcac
    10081 cttggtcgcg ggcgcatccg gcggcagcac cacgaccgcg gcgagcccgt gccggcgggc
    10141 ggcgagcgcg acggccagcg cgtggttgcc ggtgctctgg cagacgacgc cggtcgcacc
    10201 cccggccgcg gccagccgcc ccacggcgac ggtcgcgccc cgcatcttgt acgagccgcc
    10261 gtcctgcagg ttctccgcct tgaacagcag ccgtgtcccg gcccgggcgt cgaggcccgc
    10321 cgagcgcacc acgggcgtac gcacgacccg cccgtcgagg aaccgcgccg ccgcctccac
    10381 gtcggagcgc gcgagccgcc gcgtgaccgc catccccatg cccgttcccc cgctcgatcg
    10441 accgacgggc ccgagtctca cagcgaacgc ccggcgcggc ctcggtccgc gggtggatca
    10501 tgggcccggc gggtctgcga ctttcggcgg atacgctcgg cgcatgacgc cccggcccgc
    10561 accgcctccc tccccgcggg tccggcccac cgccgcggag gtcgcggcgg ccgcgggccg
    10621 gacgatcccg gacctggtcg gcccgggtct gcgggtgctg ttctccggta tcaacccgag
    10681 cctgtactcg gccgcgaccg gccaccactt cgcgcgaccc ggcaaccgct tctggccggc
    10741 cctgcaccgc tccggcttca caccgcggct gctgcacccg tccgagcagt ggtcgctgcc
    10801 ggaggccggg ctcggc
//
