LOCUS       NZ_BMMP01000001        10984 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces daqingensis strain CGMCC 4.7178 sequence01, whole
            genome shotgun sequence.
ACCESSION   NZ_BMMP01000001
VERSION     NZ_BMMP01000001.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces daqingensis
  ORGANISM  Streptomyces daqingensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 591356)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 66X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014645895.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:14:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,602
            Genes (coding)                    :: 5,509
            CDSs (with protein)               :: 5,509
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 93
            CDSs (without protein)            :: 93
            Pseudo Genes (ambiguous residues) :: 0 of 93
            Pseudo Genes (frameshifted)       :: 35 of 93
            Pseudo Genes (incomplete)         :: 69 of 93
            Pseudo Genes (internal stop)      :: 2 of 93
            Pseudo Genes (multiple problems)  :: 13 of 93
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:17:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 425980
            Orig. end                         :: 436964
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMMP01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5984)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            1535..2104
                     /locus_tag="IEY56_RS01895"
                     /old_locus_tag="GCM10012287_03830"
     CDS             1535..2104
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEY56_RS01895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10012287_03830"
                     /product="hypothetical protein"
                     /protein_id="WP_189035250.1"
                     /transl_table=11
                     /translation="MPAADPVAATPTRTARPAPVRTRDVLVLGTAEVAYCVTDALRQAG
                     LSVGSWLAPADACHHRTREHIRRDLADPATLRSWSSAAVVISVWHGSARHSHPGEVVEP
                     LVDAALERQAPHWLRLTRGVTDGGPQEAQAAVDHDPYRSTCYLPPGARAHLTRLRDQAD
                     PDGVYARTLTALLNVLPLTPGCSPRF"
     gene            2303..3808
                     /locus_tag="IEY56_RS01900"
                     /old_locus_tag="GCM10012287_03840"
     CDS             2303..3808
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546261.1"
                     /locus_tag="IEY56_RS01900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10012287_03840"
                     /product="cytosine permease"
                     /protein_id="WP_268241311.1"
                     /transl_table=11
                     /translation="MEANGLNVIGDAERKGRPAQLFWPWFGSNVSVLGLSYGSFALGFG
                     IDFWQALAAGVAGIVFSFLLCGFIAVAGKRGSAPTMVLSRAAFGVRGNRLPSAVSWVLT
                     VGWETVLVILATLATSTVFDRLGWGGGTSTKLAALVVVSALTVLTGVVGFDLIMRMQSA
                     ITVVTAALTVVYIALVAGEIRWETAGSLPAGSAQEVVGALVFLMTGFGLGWVNAAADYA
                     RYLPRRTSGAAVAGWTTFGASLAPLVLLVFGLLLAGSSPQLSEAIAADPIGALTTLLPT
                     WFLVPFALVAVLGLVGGAVLDIYSSGLALLALGVRVPRYAAAALDGLLMILGTVYVVFF
                     SEDFLGSFMGFLTTLGVPVAAWCGIMLADVALRSRPYDDADLFSPSGRYGDVRWLPAAV
                     LCAATAAGWGLVTNTAAGWLSWQGYLLGPLGLGGTSGAWASANLGVLAALVSAFAVTLV
                     CHRARVKEQEAAPPSPSGPHGNAPAGKAPAAEDGEPGTAPTGS"
     gene            complement(3959..4797)
                     /locus_tag="IEY56_RS01905"
                     /pseudo=""
     CDS             complement(3959..4797)
                     /GO_function="GO:0008276 - protein methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571805.1"
                     /locus_tag="IEY56_RS01905"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTSPLPTPLPRSALVARLRAAGCVFAEDEADLLISTAGSPRELAA
                     MAGRRTSGEPLEHVLGWAWFCGMRTAVGTGVFVPRRRTEFLVRQAAEALRRSPDAAGKR
                     RPSPCSSTCAAVRARSGRRSPPRRTGTSNCTPATSIRPPYGAPGATSPPTAGTSTTATC
                     TSRCPPGCADAWTFSWPTCPTSRRTRCPSFRRRPASTNPAWRWTAARTGSTCSAGSPPV
                     LPPGWRRAVPCWWRRANGRRPALWRRSRTAGWRPDR"
     gene            complement(5001..5984)
                     /locus_tag="IEY56_RS01910"
                     /old_locus_tag="GCM10012287_03860"
     CDS             complement(5001..5984)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="IEY56_RS01910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10012287_03860"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_189035251.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-19, bitscore: 65.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATPAVPDLPESTAYSAPVPREFVHKAAHTEVLLTGWRRLGERE
                     FEVGAQWPRDHGYWRTGRGGTQDPMLLVETTRQVLPLLSHAVFRVPPGHQLIWSRHHCA
                     FAAPLLRVTDRPAEVVLRLSVSRPPLPGGRLRRLAVRMTAACEGRTLGTASTVFSAHSP
                     EVYARLRGPRTGPAEAMSAALPPPEPVVPALVARESERDVVLAPAEDPERRQLRVVTSS
                     PWLFDHPVDHVPGMLLMEAARQCAHLVLRGRQSRLLHLVRMEAEFERYVDLDEPAWVDW
                     SQPSSRPDSPAEAGEETLTVAITQRAVRAFTAVVGFAAGSILPGTG"
     misc_feature    complement(5655..5657)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6087..6704
                     /locus_tag="IEY56_RS01915"
                     /old_locus_tag="GCM10012287_03870"
     CDS             6087..6704
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986960.1"
                     /locus_tag="IEY56_RS01915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10012287_03870"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_189035252.1"
                     /transl_table=11
                     /translation="MQQRAVETRQLILEKAAEVFDEGGYRAARIADIMHRAGITQGALY
                     FHFSSKLDLARTVMLAQRDAVVLPPGPDGLQRLIDTTLLLAFELQTNKLLRAGVRLAVE
                     QEEIGFVDDAPYREWAQVFRGQLEAAAKRGELRSHVEVDRLAVLLVGCYTGVQHFSKLA
                     NARADLPDRIADLWRYMLLGIAPPQVAAEMVVNPEHPMLAAG"
     gene            6716..7669
                     /locus_tag="IEY56_RS01920"
                     /old_locus_tag="GCM10012287_03880"
     CDS             6716..7669
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133276.1"
                     /locus_tag="IEY56_RS01920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10012287_03880"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_229711466.1"
                     /transl_table=11
                     /translation="MLSGATGFIGSAVLARLSTHAAPGNRERPSAAGELAVRALTRRHV
                     ALPAGVEQVHADLARPESLAGVCEGADVLVSAASHVGPDEQRCGLVNDVGTAALMEEAR
                     RAGVQRVLHLSTTAVYGRGPHRGANVTELEPAPGSAASRSRLAGETHALRQGALVLRAG
                     LVTGEGDRWVVPALAELARRVPGLWLGGTGRLSLVAVEDLARLVTAAALRLPAAGGAVL
                     HAVHPQPVRLRDLLRTLAGLGLLPPLSGELTWPECLSLMRANPGRIRERQLELLAQDHF
                     YGGESLWDACRCDPGPGPLARLPGAAAWYRRQLGLS"
     misc_feature    7751..7771
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.62"
                     /tool="antismash"
     gene            7859..8518
                     /locus_tag="IEY56_RS01925"
                     /old_locus_tag="GCM10012287_03890"
     CDS             7859..8518
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.5"
                     /locus_tag="IEY56_RS01925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10012287_03890"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_189035253.1"
                     /transl_table=11
                     /translation="MRQERAELTRHALTMAAARSFDRSGYQRTTLFEVSRAAAMSKGAL
                     SFHFGSKEELADTVRREACTRTFQATELLRAKAQPALQTLIDLTHLVAEQLANDHLARA
                     AVKLSRELETPPDPEVNPLACWRQVFTEVAGEAEKDGTLRPETTAETVAEVAFGLASAA
                     HTDQTDGAADPHERLSAHWQLLLYGLVSQPGPEHLWIAGTESDWLRVSGCELQKTE"
     misc_feature    complement(8645..8661)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.42"
                     /tool="antismash"
     misc_feature    complement(8803..8816)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.09"
                     /tool="antismash"
     misc_feature    complement(8825..8834)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.42"
                     /tool="antismash"
     gene            8860..9678
                     /locus_tag="IEY56_RS01930"
                     /old_locus_tag="GCM10012287_03900"
     CDS             8860..9678
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037627.5"
                     /locus_tag="IEY56_RS01930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10012287_03900"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_189035254.1"
                     /transl_table=11
                     /translation="MPRLDGPPHRRQDLQTFLREARARVDPAEHGLERPSDRRGRQVPG
                     LTQPQMDVLLHSRTGTYGSFERGVKTNPSTDYLDRLARYLKLNPQEWESLWRHTYGHNP
                     PYALDPGAGLDVAPVWRRVVHEMTTMAFLSNICWDLLDCNSAFAEIFPDGKPPENVMRW
                     MLLDDVARDEVLMDWEDEWAPVIIPQLVAVRRAYPADPTLQELENDLHGDPRAARVRRR
                     IAAAFTRPDSDDRPLRHPVLGPGRARLGAAEPLVNPGARLMVVEFEPDHP"
     gene            9832..10491
                     /locus_tag="IEY56_RS01935"
                     /old_locus_tag="GCM10012287_03910"
     CDS             9832..10491
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEY56_RS01935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10012287_03910"
                     /product="hypothetical protein"
                     /protein_id="WP_189035255.1"
                     /transl_table=11
                     /translation="MRTRTRCKAAGASCGEPKGLLPGEPGGWHRGGRCPRCRTARWAEL
                     ERATRLGPDRREAVLAALRAGVTLDAPAGALGVPIEHLAAFALRDSEVQSALAGHSLEQ
                     QLRARRHDFLTALRETEGDRELAAWAVVLDVLEAVEWLADPAYAAEEALLLTAVAERVS
                     RPRRRIADELLDRAAELLETGATITEAARRVGVATGTLRSRSGQHPRLAAALPPKR"
     misc_feature    complement(10843..10856)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.76"
                     /tool="antismash"
     misc_feature    complement(10862..10876)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.1"
                     /tool="antismash"
ORIGIN
        1 gacctgcccg atcaggagcg ggaggacctg ctgcacctgg caggggagac gctgtggtgg
       61 gggccgacag aggccgcgcg gctcgtcggc tggcaccggc agaccctcgc ccagcggctg
      121 gaccgcctcg ccgcgctcac cggcctggac ccccggcggc gctggcagca gagcgcgctg
      181 tatctcgcgg tgcgtctggc cgctctgccg cccccaccgg tgctcgaccg caccgtcacc
      241 ctccggcagg tcctggacca cccgggggcc cgcgcgtggg cggacgaaag gctggacggc
      301 ctgaccccgg acctgaggga gacgctcgcc gcctgggtcg cggccgacat ggacaaggac
      361 gcggcagccg ccgcgctggg catcactccg cgcgccgcgt accggcggct ggggcgggcg
      421 gtcaccgcga cgcggcagga gctgacgcgg tacccggggc ccgcgtcgga gacggccctg
      481 gcgctctgcg tcaccggaga cgtcatgatg ggagatgtcc cggcgatgga ggaccggcat
      541 gtgagcgggc aggccggggg aggggagcgc acggtggacg tatccgaggt ggacaccggc
      601 cgtccgcacg tcgcccgggt ctacgactac ctcctcggcg ggaactgcaa ctggcccgcc
      661 gaccgggagg ccgccgaacg gactctcgcg atcaacccgc acaccaccac ggcggtacgg
      721 gagaaccggc tcttcatgca gcggtccgtc cgcaccctca ccgccgaggt gggcatccgc
      781 cagttcctcg acctgggctc gggcatcccc acctccccga atctccacga gatcgcgcag
      841 ggggccgcgc ccggcgcccg cgtcgtctac gtcgaccacg acccgatcgt cctcgcccac
      901 gccgcggccc tgctgcgcag caccgcccgc ggccggacgg cgtacatcga ggccgacgtc
      961 ctcgacacgc gctccctgtt ctccgcgccc gggctgcgcg acgtcctcga cctggacgag
     1021 cccgtcgccg tctgcatcaa cgctctcgtc cacttcctgg acgacgacga ggcgggaaag
     1081 ctcatccgcg acgtgcggcg gcacgtcgcc cccggcagct acgtcaccct cacctcgatg
     1141 accgccgact acgcctggaa cctcatcggc agcgtgcagg cgtactacag ctccacggtc
     1201 agtccgctga tcatccgtac gcacgaggag atcgaggcgc tgttcgcggg catggagctg
     1261 ctggaccccg gcgtcgtctc cctccccgac tggcggcccc tgcccggcgc gggcgtgccg
     1321 ccgccgcccg acgaggtcaa ctgctacggg ggcgtcgccc gcgtcaagta gcggttcgca
     1381 gcgctcctcc cgggccggcc cggatatcga ccggtgtacc ggccccggat cgaccgatcc
     1441 tcccttctcc ggcctccgcc gggtgcctac ggtcgtagga gaccgattgc cccgagatgc
     1501 ctttccccga caccaggagc ggagggacga gaccatgccg gctgcagacc ccgtagccgc
     1561 cacccccacc cgcaccgcgc ggcccgcacc ggtgcgtacg cgggacgtgc tcgtgctggg
     1621 gaccgcggag gtcgcctact gcgtcaccga cgcgctgcgc caggccgggc tcagcgtcgg
     1681 cagctggctc gccccggccg acgcgtgcca ccaccggacc cgtgagcaca tccggcgcga
     1741 cctggcggac cccgccaccc tgcgctcgtg gtcctcggcc gcggtcgtca tcagcgtctg
     1801 gcacggctcc gcgcggcact cccacccggg tgaggtcgtg gagccgctcg tcgacgccgc
     1861 cctggagcgc caggcgcccc actggctgcg gctcacgcgc ggtgtcaccg acggcgggcc
     1921 gcaggaggcg caggccgccg tcgaccacga tccgtaccgg tcgacctgct acctgccgcc
     1981 cggggcccgg gcgcacctga ccaggctgcg cgaccaggcc gatccggacg gcgtctacgc
     2041 gcggacgctg accgcgctgc tgaacgtcct gccgctgacc cccgggtgca gcccgcgctt
     2101 ctgagccctg ccccgccggc ccgcccccgc gcccccgtcc ctcaacttcc ctgcgcggag
     2161 gcccacttga cggcatgctc agcgcacgcg acgctccctc tgacttcttg atccggcgat
     2221 acgggggacc actgtgcctg gggcaggagc ggacgtaccg ggcgcggcgg cggacggtga
     2281 ccgtcccgcg aggaagatca ccgtggaggc caacgggctc aacgtcatcg gggacgccga
     2341 acgcaagggc cgccccgcgc agctgttctg gccctggttc gggtcgaacg tctcggtgct
     2401 gggcctgagt tacggctcgt tcgcactggg attcggcatc gacttctggc aggcactggc
     2461 cgccggggtc gcggggatcg tcttctcgtt cctgctgtgc ggcttcatcg ccgttgcggg
     2521 caagcgcgga tccgcgccga cgatggtgct cagccgggcg gcgttcggcg tgcggggcaa
     2581 caggctgccg tccgcggtct cctgggtgct gaccgtcggc tgggagaccg tgctggtgat
     2641 cctcgcgacg ctcgccacgt ccacggtctt cgacaggctg ggctggggcg gcggcacgtc
     2701 gacgaagctc gccgcgctgg tcgtcgtctc ggcgctgacc gtcctgaccg gagtcgtcgg
     2761 attcgacctc atcatgcgga tgcagtcggc gatcacggtc gtgacggctg ccctgaccgt
     2821 cgtctacatc gccctggtcg ccggggagat ccgctgggag acggccggga gcctgcccgc
     2881 cggctcggcg caggaggtcg tcggggcgct ggtgttcctg atgacgggct tcggtctggg
     2941 ctgggtcaac gccgccgcgg actacgcccg ttacctgccg cgccggactt cgggcgccgc
     3001 cgtggcgggc tggaccactt tcggggcgtc cctcgcgccg ctcgtccttc tggtcttcgg
     3061 gctgctgctc gccggttcct ccccgcagct cagcgaggcg atcgccgccg accccatcgg
     3121 cgccctgacg acgctgctgc cgacctggtt cctcgtgccg ttcgcgctgg tcgccgtgct
     3181 gggcctggtc ggcggcgcgg tgctggacat ctactcctcg ggcctcgccc tgctcgccct
     3241 gggggtgcgg gtgccgcgct acgcggccgc ggcgctggac gggctgctca tgatcctcgg
     3301 aacggtctac gtcgtcttct tctccgagga cttcctcggc tccttcatgg gtttcctcac
     3361 cacgctcggc gtgcccgtgg ccgcctggtg cggcatcatg ctggccgacg tggcgctgcg
     3421 ctcccgtccc tacgacgacg cggacctctt ctcgccctcc ggccgctacg gcgacgtgcg
     3481 gtggcttccg gccgcggtgc tgtgcgcggc cactgccgcc ggatggggcc tggtgaccaa
     3541 caccgccgcc ggctggctgt cctggcaggg ctatctgctg gggccgctgg gcctcggcgg
     3601 cacctcgggg gcgtgggcgt ccgccaacct cggggtgctc gcggctctcg tctcggcgtt
     3661 cgccgtgacg ctcgtctgcc accgcgcccg ggtcaaggag caggaggccg cgccgccttc
     3721 gccgtccggg ccgcacggga acgcgcctgc ggggaaggcc cctgcggccg aggacgggga
     3781 gcccggaacg gccccgaccg gcagctgacg acgccgagtt cgggactcca cggagggacc
     3841 ggggctacgg cgggaacgcg gccctcttgc ggcagccggg aacggcgccc gccgccgcgt
     3901 aagaagaacg ggtgccgata agcgcacgtc gccgtccggg ccggcgagtt cgacgtgttc
     3961 aggcgcggtc gccgtcccgc cgcgtcgcgg tgatgacagt cgcgtcgaac tcctcgcacg
     4021 tcaccgatcg ggccgccagc ccgcagtccg cgaccgcctc cagagcgcgg ggcgcctgcc
     4081 gttcgctcgt ctccaccagc agggaaccgc ccggcgccag ccaggcggga gcaccggcgg
     4141 cgacccggcg gagcacgtcg agcccgtccg ggccgccgtc cagcgccacg cggggttcgt
     4201 ggaggcgggc ctccggcgga aggaggggca cctcgtccgc cgggacgtag ggcacgttgg
     4261 ccacgagaac gtccacgcgt ccgcgcagcc cggcgggcag cggctcgtac aggtcgccgt
     4321 ggtggacgtg cccgccgtag gcggcgacgt tgcgccgggc gcaccgtacg gcggccggat
     4381 cgacgtcgcc ggcgtgcagt tcgacgtgcc cgtccgcctg ggaggcgagc gccgccccga
     4441 ccgcgcccga accgcagcac aggtcgacga gcacggggac gggcgccgct tcccggcggc
     4501 gtccggtgac cggcggagag cctcggcggc ctgacgtacg aggaactccg tgcgccgccg
     4561 gggcacgaac acgcccgtcc cgaccgccgt ccgcatcccg cagaaccagg cccagcccag
     4621 gacgtgttcg aggggttcgc ccgatgtccg gcggccggcc atcgcggcca gttcgcgcgg
     4681 cgaaccggcg gtggagatca ggaggtccgc ctcgtcctcg gcgaacacgc agccggcggc
     4741 gcggaggcgg gcgacgagag cggaacgtgg gagaggagtg gggagcggcg aagtcatgcg
     4801 ggcctttcga ctggtgccac gggggctccc tgccgcctac gccagtcccg gccgggcggc
     4861 gggggagagg accccttgtc gtgctgcggt gatcggtccc acctcctcat tcgcgccgta
     4921 caggaagcac ggacgatacc cgagcgtgcg gctctgcgtc cggggtgggc ccggccgcct
     4981 ccgcccgtcc ccgggcgtcc tcaccctgtg ccgggaagga tcgatccggc agcgaagccc
     5041 accacggcgg tgaaggcccg cacggcgcgc tgggtgatcg cgacggtcag cgtctcctcg
     5101 ccggcctccg cgggggagtc cgggcgggag gagggctgcg accagtcgac ccacgcgggt
     5161 tcgtccaggt cgacgtagcg ttcgaactcg gcctccatgc gcaccagatg gagcagacgg
     5221 gactgcctgc cccggagcac gagatgagcg cactgcctgg ctgcctccat cagcagcatg
     5281 ccgggtacgt gatcgaccgg atggtcgaag agccacgggc tggaggtgac gacgcgcagc
     5341 tgccggcgct ccgggtcctc ggcgggggcg agaacaacgt cccgttcgct ctccctcgcc
     5401 acgagcgcgg ggaccaccgg ctccggtggt ggcagggcgg ccgacatcgc ctcggccggg
     5461 ccggtgcggg gaccgcgcag ccgcgcgtac acctcggggg agtgggcgct gaagacggtc
     5521 gaggccgtgc ccagggtgcg tccctcgcat gcggcggtca tcctcaccgc cagtctccgc
     5581 agccggccgc cgggcagcgg cggccgggac acggacaggc gcaggacgac ctcggcgggt
     5641 cggtccgtca cacgtaacaa gggtgcggcg aacgcgcagt ggtggcggct ccagatcaac
     5701 tggtggcccg ggggcacgcg gaagaccgca tgggaaagca gcggcagcac ctgccgggtc
     5761 gtctccacca gcagcatcgg atcctgcgtt ccgccgcggc ccgtccgcca gtagccgtgg
     5821 tcccgcggcc actgggcccc cacctcgaac tccctctctc cgagccgacg ccagccggtc
     5881 agcagcacct ccgtatgcgc cgccttgtgc acgaactcgc gggggacggg tgccgagtag
     5941 gctgtgcttt ccggcaggtc gggcactgcg ggggtggcgg tcatcggcgg tcctccgggt
     6001 gggatggtgt ccagagtgag tgacctgtgc cggaaaccat agaactttgg tggcgaggcc
     6061 ggatggtggc agcgagaacg aaaccgatgc agcaacgcgc cgtcgaaacg cggcagttga
     6121 tcctggagaa agcggcggag gtcttcgacg aggggggcta cagggcggcg aggatcgccg
     6181 acatcatgca ccgcgccggc atcacccagg gcgccctgta cttccacttc tcctcgaagc
     6241 tcgacctcgc ccgcacggtg atgctcgccc agcgcgacgc cgtggtgctt cccccggggc
     6301 ccgacggtct ccagcggctc atcgacacca cgctcctgct cgccttcgag ttgcagacca
     6361 acaagctgct ccgcgccggg gtgcggctcg ccgtggagca ggaggagatc ggcttcgtcg
     6421 acgacgcgcc gtaccgcgaa tgggcgcagg tcttccgcgg ccagctcgaa gcggccgcga
     6481 aacgcgggga gttgcgcagc catgtcgagg tggaccgcct cgcggtgctg ctggtgggct
     6541 gctacaccgg cgtgcagcac ttctcgaagc tcgccaacgc ccgcgccgac cttcccgacc
     6601 gcatcgccga tctgtggcgc tacatgctgc tgggtatcgc cccgccgcag gtcgccgcgg
     6661 agatggtcgt caatccggag cacccgatgc tcgcggcagg ctgatcgtgc gcgtcgtgct
     6721 gagcggtgcc acggggttca tcggctcggc tgtcctcgca cggctcagca cgcacgcagc
     6781 gcccggcaac cgcgaacgtc ccagtgcagc aggggagttg gccgtacgcg ccctcacccg
     6841 caggcacgtt gcgctgcccg ccggggtgga gcaggtccac gccgacctgg ctcggcccga
     6901 gtccctggcc ggcgtctgcg agggagcgga cgtactcgtg agcgccgcct cccacgtcgg
     6961 tccggacgag caacggtgcg gcctggtcaa cgacgtgggt acggccgcgc tcatggaaga
     7021 ggcgcgcagg gccggcgtcc agcgggtcct gcacctgtcg acgacggccg tctacggccg
     7081 gggcccccac cgtggcgcga acgtcaccga gttggagccg gctcccggat ccgccgccag
     7141 ccggagccgg ctcgccgggg agacgcacgc gctgcgtcag ggcgccctcg tactgcgtgc
     7201 cggactcgtg accggtgaag gcgaccgatg ggtcgtgccg gcgctggccg aactcgcccg
     7261 ccgtgtgccg gggttgtggc tgggcggaac ggggcgcctc tcgctcgtgg ccgtggagga
     7321 cctggcacgg ctcgtcacgg ccgccgcgct ccgtctcccg gcggcgggcg gagccgtcct
     7381 ccacgcggtc catcctcagc ccgtacgtct gcgggacctt ctccggaccc ttgccggcct
     7441 ggggctgttg ccgccgctga gtggggagtt gacctggccg gaatgcctct ccctgatgcg
     7501 tgcgaacccc ggacggatcc gtgaacggca gctcgaactg ctcgcacagg accacttcta
     7561 cggcggggaa tcgctctggg acgcatgccg atgcgacccg ggacccggcc cgttggcgag
     7621 actgcccggc gcagctgcct ggtaccgccg ccaactcggt ctcagctgag gcatgttgcg
     7681 caagcacgtc gacggggcat cactcttcca ctccttctcc tgcggctctg tagtcccttc
     7741 cagcgcatga aaacagaaca ctttgtatgt ttgtctgggg cggcagttct ccccgtcagc
     7801 tgctgcccct gacggtccgt ttccccgcgg acccgtacgc acgaagggaa caggctccat
     7861 gaggcaagag cgcgcagaac tcacgcggca cgcactgacg atggccgcgg cgagatcctt
     7921 cgaccgctcc ggctaccagc gcaccacact cttcgaggtg agccgtgccg cggcgatgag
     7981 caagggcgcc ctgtccttcc acttcggcag caaggaggaa ctggccgaca ccgtccggcg
     8041 cgaggcgtgc acccgtacct tccaggcgac cgaactgctg cgggccaagg cccagccggc
     8101 cctgcagacg ctcatcgacc tcacacacct cgtggccgag cagctggcca acgaccatct
     8161 ggcccgggcc gcggtgaagc tgtcccgcga gctggagaca ccgccggacc ccgaggtcaa
     8221 cccgctcgcc tgctggcggc aggtcttcac cgaagtggcc ggggaggccg agaaggacgg
     8281 gacgctgcgc cccgagacga cggcggagac ggtggcggaa gtggccttcg gccttgcctc
     8341 ggcggcccat accgaccaga cggacggagc agccgatccg catgaacgcc tctcggcgca
     8401 ctggcagttg ctgctgtacg gactggtctc ccagccgggt cccgagcatc tgtggatcgc
     8461 gggcaccgag tcggactggc tgcgtgtctc cgggtgcgaa ctgcagaaga ccgagtgatc
     8521 cggccgggct tggctgaagc gatgtccaag cggtggcttg cggggtcgac tccggccggc
     8581 cgtggtccgg gagccggggg aggggcgtca gccgggcagg ccgccccgcg gcggatggga
     8641 gacctgccga cctcaggtgc aggcgttccc ctgacccctc ttcgccctga agagccgagg
     8701 agtcccgcca agcatcacgt ctcgcgccac gctgtccccc gacggcagcg tggtgccctc
     8761 cgatcatgcc gtgtgtgact ggcacctttc caggcgcgac cttttacgtg taagaacggg
     8821 gtactcaaaa aaagcccgca cagtccgagg agcgccagaa tgccccggct cgacggcccg
     8881 ccccaccgcc ggcaggacct gcagacgttc ctcagggagg cccgggcacg ggtcgatccc
     8941 gccgaacacg ggctggagcg gccgagcgac cgccggggac gccaggttcc cggcctcacc
     9001 cagccgcaga tggacgtgct gctgcacagt cggacgggca cctacggcag cttcgaacgc
     9061 ggcgtgaaga cgaacccgtc gaccgactac ctcgaccgtc tggcgcgcta tctgaagctg
     9121 aacccgcagg agtgggaatc gctttggcgg cacacctacg ggcacaaccc tccctacgcg
     9181 ctggaccccg gcgccggtct ggacgtcgcg cccgtgtggc gccgcgtcgt ccacgagatg
     9241 acgacgatgg ccttcctgag caacatctgc tgggacctgc tggactgcaa ctcggccttc
     9301 gcggagatct tcccggacgg caaacccccc gagaacgtca tgcggtggat gctgctggac
     9361 gacgtggcgc gcgacgaagt cctcatggac tgggaggacg agtgggcgcc ggtgatcatc
     9421 ccgcagctcg tggccgtccg gcgcgcctat ccggccgatc cgacgcttca ggagctggag
     9481 aacgacctgc acggcgaccc gcgtgccgca cgcgtccggc gcaggatcgc cgctgccttc
     9541 acccggcccg acagcgacga ccgcccgctg cggcaccccg tcctcgggcc cggcagggca
     9601 cggctcggtg cggccgaacc gctcgtcaac cccggggcgc ggctcatggt cgtcgagttc
     9661 gagccggacc acccctgacc tgccccggag ggcgggcccg accgtgctct gccgtgcgcc
     9721 ggagggcgag cgtccgcttg ccgtggcggc ggtgggtagc atgtccgcca tgaggagaag
     9781 ggggaggttc gcggccccgg ccggcccgga ccgcggtcca gcggagcccc tgtgagaacg
     9841 cgtacacggt gcaaagcggc tggagccagc tgcggtgagc cgaagggcct gctccccggc
     9901 gagcccgggg gatggcaccg gggcggacgc tgccccaggt gccgtaccgc gcgctgggcg
     9961 gagctggagc gtgcgacccg cctcggtccg gaccggcgtg aggccgtgct cgcggcgctc
    10021 cgcgcaggcg tcaccctcga cgcaccggca ggcgcactgg gagttcccat cgagcacctc
    10081 gccgcgttcg ccctccgcga cagcgaggtc cagtccgccc tcgcgggaca ctcgctcgaa
    10141 cagcaactcc gggccaggcg gcacgacttc ctgacggcgc tcagggagac cgaaggcgac
    10201 agggagctcg ccgcctgggc cgtcgtcctg gacgtgctgg aggctgtcga gtggctcgcc
    10261 gatccggcct acgcggccga agaggcgctg ctgctgaccg cggtcgccga gcgggtgagc
    10321 cgcccccggc gccggatcgc cgacgaacta ctggacaggg cggccgagtt gctcgagaca
    10381 ggtgccacca tcaccgaggc ggcacgcagg gtgggcgtcg ccacgggtac gctgcgcagc
    10441 cgcagcgggc agcatccccg cctggcggcg gccctcccgc cgaaacgctg agcgccccca
    10501 attcgcagcc cgcagtccgc aacccggggc ggcccgcagc tcgcggtcgg cagcgcccgg
    10561 ggacggcgcg ccgcagggcg gcgcgatgcc cgtccaggac gccggtggcc gcgcaccggc
    10621 gccgcttccc cgtccgaagg ggcaggtcgt ggcggcggcc cgtacgcgaa gtgttcgttg
    10681 aacaggatgc gcaactgtct gcggtacgcg cagcgcccgt gatacgcaca tggtggaacc
    10741 cacggcacac gtgttcgcag ggcgtcgccg cctcgctgcc gctgcggccg ggggttccgc
    10801 cgcgggtctc cggcggcaga aggccggccg ccgggtccgt catgaaagtg catgcgcacc
    10861 gtctgcagaa ggagaccacc atggcgcacg cgagcggcat gccgccgacc tctcacgcca
    10921 acccctcagc caggcgttca cgccgcaaga gccccgcccg gatcgccctc gaagtggccc
    10981 tgtg
//
