LOCUS       NZ_BMMM01000007        10405 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces albiflavescens strain CGMCC 4.7111 sequence07, whole
            genome shotgun sequence.
ACCESSION   NZ_BMMM01000007
VERSION     NZ_BMMM01000007.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces albiflavescens
  ORGANISM  Streptomyces albiflavescens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 472558)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 100X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014645835.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:24:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,828
            CDSs (total)                      :: 9,745
            Genes (coding)                    :: 9,386
            CDSs (with protein)               :: 9,386
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 359
            CDSs (without protein)            :: 359
            Pseudo Genes (ambiguous residues) :: 0 of 359
            Pseudo Genes (frameshifted)       :: 102 of 359
            Pseudo Genes (incomplete)         :: 321 of 359
            Pseudo Genes (internal stop)      :: 17 of 359
            Pseudo Genes (multiple problems)  :: 80 of 359
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:54:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 93756
            Orig. end                         :: 104161
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMMM01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(365..1213)
                     /locus_tag="IEY95_RS20225"
                     /old_locus_tag="GCM10011579_040970"
     CDS             complement(365..1213)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="IEY95_RS20225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_040970"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_189187468.1"
                     /transl_table=11
                     /translation="MNSVLGRRTRILAATTATAGLLLVAGCSSDGGGGKATAKGVPVVK
                     AGQLTTCTHLPYPPFQSEIGGKVQGFDVSLIDLVAKDLGVKQAIVDTPFENFKTGAFLN
                     SGQCDLAAAGMTITEERKKNVDFSDPYFDATQAVLVDKKSGINSLADVKSKGKKLGAQA
                     QTTGEDYVTKKGFDPVSFESSDAVLNGLRTGQVQAVVIDYPVVQGWLKDKANADAFKVV
                     DNLNTGEQYGFTVKKGNTKLLAAINKAIKGAKADGTYKKLYEQWIGPYDQSAASAAPSA
                     S"
     gene            1486..2586
                     /locus_tag="IEY95_RS20230"
                     /old_locus_tag="GCM10011579_040980"
     CDS             1486..2586
                     /NRPS_PKS="Domain: Aminotran_5 (74-299). E-value: 1.9e-27.
                     Score: 88.0. Matches aSDomain:
                     nrpspksdomains_IEY95_RS20230_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="IEY95_RS20230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_040980"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_189187783.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.3e-21, bitscore:
                     71.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSATHFASIEDRVARLLSTEQDVVIMQGEALLPLE
                     GAIRATAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLAVPFHTAVTAAQIREAFAEH
                     PSIDFVSLVHAEAATGNTNPVAKIGEVVREHGALFYLDAVASIGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPTAPRRSYLSLLDWKERWVDGGRKALLHAP
                     AQLEMLALEACVERIEGEGLAAVMDRHASAAAATRAGAVALGGGLEPYVYAPSDAAPVA
                     TTLRVPVGVDASALVAKALAADPVLPLVAGGGALATSMIRVNHYGPDATQGVVHSSLAG
                     LGAALAEFGVAVDLEGARRAVAGVWG"
     aSDomain        1708..2382
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY95_RS20230_Aminotran_5.1"
                     /evalue="1.90E-27"
                     /label="IEY95_RS20230_Aminotran_5.1"
                     /locus_tag="IEY95_RS20230"
                     /protein_end="299"
                     /protein_start="74"
                     /score="88.0"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAVTAAQIREAFAEHPSIDFVSLVHAEAAT
                     GNTNPVAKIGEVVREHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPTAPRRSYLSLLDWKERWVDGGRKALLHAPAQLEMLALEACVERI
                     EGEGLAAVMDRHASAAAATRAGAVALGGGLEPYVYAPSDAAPVATTLRVPVGVDASALV
                     AKA"
     misc_feature    2911..2926
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2947..2962
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.13"
                     /tool="antismash"
     misc_feature    3044..3056
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3081..3644
                     /gene="ectA"
                     /locus_tag="IEY95_RS20235"
                     /old_locus_tag="GCM10011579_040990"
     CDS             3081..3644
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375120.1"
                     /locus_tag="IEY95_RS20235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_040990"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_189187469.1"
                     /transl_table=11
                     /translation="MTAAHADLQAEFLEMPEGLRIDRPEVADGAALWRIAKDSRTLDLN
                     SSYSYLLWCRDFAGTSAVARDEEGTPVGFVTGYIRPERPRTLLVWQVAVDGAYRGRGLA
                     AALLDGLTARVAEESEHAPTTVETTITPGNTASERLFTSYARRHGATVEREVLFQAGQF
                     PDGTHDPEVLYRIGPLSPASPLSR"
     gene            3692..4963
                     /gene="ectB"
                     /locus_tag="IEY95_RS20240"
                     /old_locus_tag="GCM10011579_041000"
     CDS             3692..4963
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.9e-73.
                     Score: 238.3. Matches aSDomain:
                     nrpspksdomains_IEY95_RS20240_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="IEY95_RS20240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041000"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189187470.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.5e-78, bitscore:
                     259.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMYDEDGHAY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLEAFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGALAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARP
                     EWLRALADLCARRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALEAYWADGSVMEKQTRARGEQIEQAL
                     ISITEENLADVKEYRGRGLVWGMEFTDKERAGRIAQRAFELGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLRTLARAVRETA"
     aSDomain        3776..4789
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY95_RS20240_Aminotran_3.1"
                     /evalue="3.90E-73"
                     /label="IEY95_RS20240_Aminotran_3.1"
                     /locus_tag="IEY95_RS20240"
                     /protein_end="366"
                     /protein_start="28"
                     /score="238.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHAYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLEAFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGALAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARPEWLRALADLCARRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSVMEKQTRARGEQIEQALISITEENLADVKEYRGRGLVWGMEFT"
     gene            5001..5405
                     /locus_tag="IEY95_RS20245"
                     /old_locus_tag="GCM10011579_041010"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="IEY95_RS20245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041010"
                     /product="ectoine synthase"
                     /protein_id="WP_189187471.1"
                     /sec_met_domain="ectoine_synt (E-value: 2e-55, bitscore:
                     180.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRSASGTWESKRIVLAKEKVGFSVHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDDETGRKYTITPGTMYLLDGHERHTMRIKEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5413..6300
                     /gene="thpD"
                     /locus_tag="IEY95_RS20250"
                     /old_locus_tag="GCM10011579_041020"
     CDS             5413..6300
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436210.1"
                     /locus_tag="IEY95_RS20250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041020"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_189187472.1"
                     /sec_met_domain="PhyH (E-value: 9.4e-40, bitscore: 131.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLTDLYPSRGATEVSVPRQDPVVWSAPGEPGPIAVSELQAYER
                     DGFLPVEELIAEDEVAVYRQELERIVGDPAIRADERSIIEPKSREIRSVFEVHRISEVF
                     ARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPQDNYKKSLQMQDAGTPSDEALTRF
                     ANRHGIKLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPFAAPVR
                     RPEFIGARDFTPVR"
     gene            complement(6426..7454)
                     /locus_tag="IEY95_RS20255"
                     /old_locus_tag="GCM10011579_041030"
     CDS             complement(6426..7454)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="IEY95_RS20255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041030"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_189187473.1"
                     /transl_table=11
                     /translation="MSTDHEGVRDLLGAWAVGALTPADQKTVPLHLAECESCAAEAERL
                     RETVRLLGGAPRPPLANGALAGIDGVLTVALRARPAAPRVASHAAPYAAAVAGLQALLR
                     ELDGHGPWGAPVVHDWDVHATVAHLIAADEPLARRLGLAARVPESPVAEGTGWADAWAA
                     RTAAVIAYEQRRTPEETVAAWTAQAAALLATPEARDPERAAHATTLMGVRLPVADHFVV
                     RAFEAWIHTDDIGRALGLAVPPPPDPHLWQLVRLAVRILGMALGPTAPPVLFAVTGGEP
                     DAQWVLGSEDEPVRAELALDPVDFCLLVGGRYAPDEVPRGAAGDDAAIRDVLERAASLA
                     WL"
     gene            complement(7558..8154)
                     /locus_tag="IEY95_RS20260"
                     /old_locus_tag="GCM10011579_041040"
     CDS             complement(7558..8154)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="IEY95_RS20260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041040"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_189187474.1"
                     /transl_table=11
                     /translation="MVVPVDPTLTDAELHRRLVYGDETALAEAYAAYGGLVRRVAVRVT
                     RSPAAAEDVAQEVFAQLWSRPYAFDPRRGSLRTWLSMLAHRRAVDWVRSEARHRKDARA
                     DDSALHAIPDAGPGPDETVVDRERSLLLHTALAELPQPQREVVHLAYFVGRTYRQAAVE
                     LGIPEGTAKTRLRSALRKLAESLADPPDPALERGA"
     gene            complement(8208..9257)
                     /locus_tag="IEY95_RS20265"
                     /old_locus_tag="GCM10011579_041050"
     CDS             complement(8208..9257)
                     /NRPS_PKS="Domain: Aminotran_5 (16-343). E-value: 8.6e-26.
                     Score: 82.6. Matches aSDomain:
                     nrpspksdomains_IEY95_RS20265_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477739.1"
                     /locus_tag="IEY95_RS20265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041050"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_189187475.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.3e-21, bitscore:
                     69.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFAPENTYLNTASTGLVPARTVAAMRTAVESVAAG
                     RPADMFADVEAARASFARLVGVPGHRVAAGASVAVYSGLIAASLPQGAEVLTAEADFSS
                     VVTPFHVRGDLKVRAVPLERLAESVRPGTALVAVSAAQSADGRIADLEAIRDAARAHGA
                     RTYIDASQAAGWLPLRADAYDYVSSVAFKWLVCPRGVAFLVVPEDLGGLTPVFAGWVAG
                     EAPWDSCYGPVEELAHSARRFDESPSLFSYAGARHSLALIEELGVANVHAHDLALADRF
                     REGLHALGHEPIPAPGSAIVSVPELGHHQPDLSRAGVEVSDRAGNLRAAFHLYNTPADV
                     DRLLDVLSG"
     aSDomain        complement(8229..9209)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY95_RS20265_Aminotran_5.1"
                     /evalue="8.60E-26"
                     /label="IEY95_RS20265_Aminotran_5.1"
                     /locus_tag="IEY95_RS20265"
                     /protein_end="343"
                     /protein_start="16"
                     /score="82.6"
                     /tool="antismash"
                     /translation="TYLNTASTGLVPARTVAAMRTAVESVAAGRPADMFADVEAARASF
                     ARLVGVPGHRVAAGASVAVYSGLIAASLPQGAEVLTAEADFSSVVTPFHVRGDLKVRAV
                     PLERLAESVRPGTALVAVSAAQSADGRIADLEAIRDAARAHGARTYIDASQAAGWLPLR
                     ADAYDYVSSVAFKWLVCPRGVAFLVVPEDLGGLTPVFAGWVAGEAPWDSCYGPVEELAH
                     SARRFDESPSLFSYAGARHSLALIEELGVANVHAHDLALADRFREGLHALGHEPIPAPG
                     SAIVSVPELGHHQPDLSRAGVEVSDRAGNLRAAFHLYNTPADVDRL"
     gene            complement(9354..10073)
                     /locus_tag="IEY95_RS20270"
                     /old_locus_tag="GCM10011579_041060"
     CDS             complement(9354..10073)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067467.1"
                     /locus_tag="IEY95_RS20270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_041060"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_189187476.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALDAFPHRDEVEVVHRSFELDP
                     GRAKGDIQPVIKMLTKKYGMSEAQAQAGEENLGVQAAAEGLEYRTRDRDHGNTFDMHRL
                     LHFAKEQGRQDELIGLLYKANFAEERTVFDDDERLVELAVAAGLDADAARKVIADPSAY
                     ADDVRADEREAAELGANGVPFFVLDRKYGVSGAQPAEVFAQALTQAWGERPPLKLIQDG
                     SADAEACGPDGCAVPQN"
ORIGIN
        1 acgaagatca acagggcggg cagaccgcgg aagatctcga tgtagatgcc ggcgagccag
       61 cggtaggggc ccaccgggga cagccgcatc agggcgatga ccatgccgag cacgagaccg
      121 aacacgaagc cggacatcgt gtagagcacg gtgttcttca gcgccagcgt gatgacgtcc
      181 gggaacatct gcttgaccag gtcccactgg gcgaactggt tcttcagacg tccccagtcg
      241 gccgtgaccg cgagggcgat cacggcggcg acgaagacga cgtactgcgc gccgcgcgag
      301 agcgcgcgct tctgacgtcg ggtcaggccg gacttgcggg gctggacttg tgcgtcggca
      361 ccgctcatga ggcggacggg gcggcggagg ccgcggactg gtcgtacggg ccgatccact
      421 gctcgtagag cttcttgtac gtgccgtcgg ccttcgcgcc cttgatcgcc ttgttgatgg
      481 cggcgaggag cttggtgttg cccttcttca ccgtgaagcc gtactgctca ccggtgttga
      541 ggttgtcgac caccttgaag gcgtcggcgt tggccttgtc cttcagccag ccctggacga
      601 ccgggtagtc gatgacgacg gcctggacct ggccggtgcg caggccgttg aggaccgcgt
      661 cggaggactc gaaggagacc gggtcgaagc ccttcttcgt cacgtagtcc tcgcccgtgg
      721 tctgcgcctg ggcgccgagc ttcttgccct tggacttcac gtcggcgagg gagttgatgc
      781 cgctcttctt gtcgaccagg acggcctgcg tcgcgtcgaa gtacgggtcg gagaagtcga
      841 cgttcttctt gcgctcctcg gtgatcgtca tgccggccgc ggcgaggtcg cactggccgg
      901 agttgaggaa cgcgcccgtc ttgaagttct cgaagggcgt gtcgacgatg gcctgcttca
      961 caccgaggtc cttggcgacc aggtcgatga gagagacgtc gaagccctgc accttgccgc
     1021 cgatctccga ctggaagggc gggtagggga ggtgcgtgca ggtcgtgagc tggcccgcct
     1081 tcaccacggg gacccccttg gcggtggcct tgcctcctcc gccgtccgag gagcagccgg
     1141 ccacgagcag cagcccggcc gtcgcggtgg tggcggccag aatgcgggtc cggcgtccga
     1201 ggaccgagtt cacggagacc tcctatgggg gaactgtggg ttccgattat aaggaaaggt
     1261 ttgagttgct caaaacaatc cgatggttgc tgggccgccc ggcctcggat ccggtgccga
     1321 aaggggtgcc ggtggtctcg tgccgggcgt agcggccgcc gaccggtacg cccctccccc
     1381 accgcgtccg agacccacgg gatcgccggt tacgctcaaa accgcgcacc cgcgtgccac
     1441 aacgcgtatc cgcgcatcga aaccgtgagc gaagagagca ccgccgtgac ccaccccttc
     1501 ctggacctgg cgccgctgag cgccacgcac ttcgcgtcga tcgaggaccg cgtggcccgg
     1561 ctgctctcca ccgagcagga cgtcgtgatc atgcaggggg aggcgctgct cccgctggag
     1621 ggggccattc gcgcgacggc cggacccggc acgaccgcgc tcaacgtcat caccggtccg
     1681 tacggacaga ccttcgggga ctggctgcgg gactgcgggg ccaccgtgat cgatctggcg
     1741 gtgcccttcc acaccgccgt caccgccgcg cagatccgcg aggccttcgc cgagcacccg
     1801 tcgatcgact tcgtgtcgct cgtgcacgcg gaggccgcca ccgggaacac caatccggtc
     1861 gcgaagatcg gcgaggtcgt gcgtgagcac ggcgcgctct tctacctgga cgcggtcgcg
     1921 tcgatcggcg ccgagccggt gctgccggac gcatggggtg tcgacctgtg cgtgatcggg
     1981 gcgcagaagg cgatgggcgg ccccgcgggc gtgtcggcgg tgtcggtgag cgagcgggcg
     2041 tgggcgcgga tggccgcgaa tccgacggcg ccgcgccgct cgtatctctc cttgctggac
     2101 tggaaggaga ggtgggtcga cggcgggcgt aaggccctgc tgcacgcgcc ggcgcagttg
     2161 gagatgctgg cgctcgaggc gtgcgtcgag cgcatcgagg gtgaggggct ggccgcggtg
     2221 atggaccgtc acgcctcggc cgctgcggcg actcgggcgg gggccgtcgc cctgggcggc
     2281 gggctcgagc cgtatgtgta cgcgccgtcc gacgccgcac ctgtcgccac gacgttgcgg
     2341 gtgccggtgg gggttgacgc gtcggcgctg gtcgcgaagg cgcttgccgc cgatccggtg
     2401 cttccgttgg tcgcgggtgg gggtgcgctc gcaacgtcga tgatccgggt gaaccactat
     2461 gggcccgatg cgacgcaggg ggtcgtccac tccagccttg cggggcttgg ggccgcgttg
     2521 gccgagttcg gcgtcgcggt ggatctggag ggggcgcgac gggctgtggc gggggtctgg
     2581 ggctgaggcc cgagcgatag ttcgccccct ccgcccctac ccgtcccgta ccagccttcg
     2641 gctgcgggtt cgtgggggct tgtcgcgcgg ttccccgcgc cccttttcag ggccgcgggg
     2701 aactgcgcaa tcttttagcg ggggtctggg ggcgcagccc ccagggacgg gacggatagg
     2761 ggcggagggg gcgaaaaact cccgcctatc cagtaattaa ttcggcgcag cttcccgcat
     2821 tcttctgccc gctttttccg tgcctaaacg caaagatttc gcgaaggcca accgacgtaa
     2881 ttcgggcaga tctcgtggcc attacgtggc tgtgacgcac tccacacccc gcccgtttcc
     2941 tccactattt ccggatgaaa acgggactta tcgccgtcga ctcacgcacc cttcacgggc
     3001 gcgtgataac acatgggcgc gccgcacgta accccgatcg acgatgcaat ttgaactttc
     3061 gctcggtaaa ttcaatccgc atgaccgccg cccacgcaga cctgcaagcg gaattcctgg
     3121 aaatgccgga gggccttcgg atcgaccgtc cggaggtggc cgacggggcc gcgctctggc
     3181 gtatcgccaa ggactccaga accctcgatc tcaactcctc gtacagctat ctgctctggt
     3241 gccgtgactt cgccggcacg tcggcggtgg cgcgcgacga ggaggggacg cccgtcggtt
     3301 tcgtcaccgg atacatccgg cccgagcgcc cgcgcacact gctcgtctgg caggtggccg
     3361 tcgacggcgc ctaccgcgga cgcgggctcg ccgcggcgct gctcgacggg ctgaccgcac
     3421 gggtcgccga ggagagcgag cacgcgccga ccaccgtgga gacgacgatc acgcccggca
     3481 acaccgcctc cgagcgcctg ttcacgtcgt acgcgcgacg ccacggcgcg accgtcgagc
     3541 gcgaagtcct gttccaggcc gggcagttcc ccgacgggac gcacgatccc gaggtgctgt
     3601 accgcatcgg tcccctctcc cccgcctccc ccctctcccg ctgacctgcc ccgatcttct
     3661 tctcccccca cgcttcgagg agcgattcgc tgtgagcatc acccagcccg acctgagcgt
     3721 cttcgagacc ctggagtcgg aggtgcgcag ctactgccgc ggctggccca cggtcttcga
     3781 ccgggcgcag ggcagccgga tgtacgacga ggacgggcac gcgtacctcg acttcttcgc
     3841 cggcgcgggg tcgttgaact acgggcacaa caaccctgta ctcaaacggg ccctcatcga
     3901 ctacctggag cgcgacggcg tcacgcacgg gctcgacatg tcgacgaccg ccaagcgggc
     3961 cttcctcgag gccttccaga atcttgtcct gcggccgcgc gatctgccgt acaaggtcat
     4021 gttcccgggg ccgaccggca ccaacgccgt ggagtccgcg ctgaagttgg cgcggaaggt
     4081 gaaggggcgc gaggcgatcg tgtccttcac caacgccttc cacgggatgt cgctcggggc
     4141 gctcgccgtg accgggaacg ccttcaagcg ggccggcgcc gggattccgc tcgtgcacgg
     4201 cactccgatg ccgttcgaca actacttcga cgggcaggtc cccgacttcc tctggttcga
     4261 gcggctgctc gaggaccagg ggtcggggct caaccagccc gccgcggtca tcgtcgagac
     4321 cgtgcagggc gagggtggca tcaatgtcgc gcggcccgag tggttgcggg ccctcgccga
     4381 cctgtgcgcg cggcgcgaca tgctgctgat cgtcgacgac atccagatgg gctgcggacg
     4441 gacgggagcg ttcttctcct tcgaggaagc gggcatcgtg cccgacatcg tgaccgtctc
     4501 caaatccatc agcgggtacg ggcttcccat gtccctgtgc ctgttcaagc ccgagctgga
     4561 catctgggag ccgggcgagc acaacgggac cttccgcggc aacaaccccg cgttcgtcac
     4621 ggccaccgca gccctggagg cgtactgggc cgacggctcc gtcatggaga agcagacccg
     4681 tgcccgcggc gagcagatcg agcaggcgct gatctccatc accgaggaga acctcgccga
     4741 cgtcaaggag taccgggggc ggggactcgt ctggggcatg gagttcacgg acaaggagcg
     4801 cgctgggcgt atcgcgcaac gcgccttcga actcgggctg ctcatcgaga cgtccgggcc
     4861 ggagggcgag gtcgtgaaac ttctcccggc gctcaccatc acccccgacg agctcgacga
     4921 ggggctgcgc accctcgccc gcgccgtacg cgagaccgcc tgacccacac cgagccacga
     4981 gagagaggca tcgcaccacc gtgatagtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtcag gtcggcctcg ggcacctggg agagcaagcg catcgtcctc gccaaggaga
     5101 aggtcggctt ctccgtgcac gagacgatcc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtgtgcg tcgagggcga ggccgagctc accgacgacg
     5221 agaccgggcg gaagtacacg atcacgcccg gcaccatgta cctcctcgac ggtcatgaga
     5281 ggcacaccat gcggatcaag gaggacttcc gctgcatctg cgtcttcaac cctcccgtca
     5341 ccggacggga ggaccacgac gagaacggcg tctaccccct gctcaccgaa cccgaggagg
     5401 tgtgagaagc gcatgaccac cctcaccgat ctctacccca gccgcggcgc caccgaggtg
     5461 tccgtgcccc gccaggaccc ggtcgtgtgg agcgcgccgg gcgagcccgg accgatcgcg
     5521 gtgtccgagc tccaggcgta cgagcgtgac ggcttcctcc ccgtcgagga gctcatcgcc
     5581 gaggacgaag tcgccgtgta ccggcaggag ttggagcgca tcgtcggcga tccggcgatc
     5641 cgtgccgacg agcggtccat catcgagccg aagtcccggg agatccggtc cgtcttcgag
     5701 gtgcaccgga tcagcgaggt cttcgcccgt ctcgtccgtg acgagcgcgt ggtcgggcgg
     5761 gcccggcaga tcctcggctc ggacgtctac gtccaccagt cgcggatcaa cgtcaagccg
     5821 ggcttcgggg ccagcggctt ctactggcac tcggacttcg agacctggca cgccgaggac
     5881 ggtctgccga acatgcggac cgtgtccgtc tcgatcgcgc tgaccgagaa ctacgacacc
     5941 aacggcggcc tcatgatcat gccggggtcg cacaagacgt tcctcggctg cgcgggcgcc
     6001 acaccgcagg acaactacaa gaagtcgctg cagatgcagg acgcgggcac gccctccgac
     6061 gaggcgctca cccgcttcgc caaccgccac gggatcaagc tcttcacggg ccgggccggt
     6121 tcggcgacct ggttcgactg caattgcatg cacggctccg gggacaacat caccccgttc
     6181 ccgcgcagca atgtcttcat cgtgttcaac agcgtggaga acgcggccgt ggaaccgttc
     6241 gcggcgccgg tgcgcaggcc cgagttcatc ggggcgaggg actttacgcc ggtgcggtga
     6301 ctccgttccg gtcggcggag ccgacccgcg gcgaagccgc catcagacac agcatcgggg
     6361 cgagggactt cacaccggtg cggtagtcgg catgcgtggc ccggcacttc acctgagtgc
     6421 cgggcctaca gccacgccag tgaagcggcc cgctccagta cgtccctgat cgcggcgtcg
     6481 tcccccgccg cgccccgggg cacctcgtcg ggtgcgtacc gcccgcccac cagcaggcag
     6541 aagtcgacgg gatccagggc gagttcggct cgtacgggct cgtcctcgga accgagcacc
     6601 cactgggcgt cgggctctcc cccggtcacc gcgaacagca ccggcggtgc cgtggggccc
     6661 agggccatgc cgaggatgcg gacggccagc cggacgagtt gccacaggtg tggatccggg
     6721 ggcgggggca cggcgagccc gagggcccgt ccgatgtcgt cggtgtggat ccatgcctcg
     6781 aaggcccgca cgacgaagtg gtctgccacg ggcagccgta cgcccatcaa cgtggtggcg
     6841 tgcgcggccc gttcgggatc cctcgcctcg ggcgtggcca gcagcgcggc ggcctgcgcg
     6901 gtccacgcgg caacggtttc ctcgggcgtc cgccgctgct cgtaggcgat cacggcggcg
     6961 gtccgcgcgg cccaggcgtc cgcccatccc gtcccctccg cgaccgggga ctccggcacc
     7021 cgcgccgcca ggcccagtcg tcgcgccagc ggctcgtccg ccgcgatcag gtgcgccacc
     7081 gtggcgtgca cgtcccagtc gtgcacgacc ggcgcacccc agggaccatg accgtccaac
     7141 tcccgcaaca gggcctggag tccggcgacg gcggcggcgt acggggcggc gtgcgaggcc
     7201 acccggggtg ccgcgggacg ggcgcgcagg gcgacagtga ggacgccgtc gataccggcc
     7261 aaggcaccgt tcgccagggg cggccgtggg gcgccgccca gcagtcgtac cgtctcccgc
     7321 agccgttccg cctccgccgc gcaggactcg cactccgcca ggtgcagggg gaccgtcttc
     7381 tggtcggcgg gggtcagtgc gccgacggcc caggccccca gcaggtcccg tacgccttcg
     7441 tggtcggtgc tcatcacatg ccgtccttcc gggccacggc cggtatggtg gccgcgcctc
     7501 gccccgctgt ggccacccta cgcacgcccg gcacggccgg gcccactcgc gccgccatca
     7561 cgcgcccctc tccagcgccg gatccggtgg gtcggccagg gattcggcga gcttgcgcag
     7621 ggcggagcgc agccgggtct tggccgtacc ctcggggatc cccagttcca cggcggcctg
     7681 tcggtaggtg cggcccacga agtaggcgag gtggacgact tccctctgcg gctgcgggag
     7741 ttcggcgagg gcggtgtgca gcagcagcga gcgttcgcgg tcgacgaccg tctcgtcggg
     7801 gccgggtccg gcgtcgggga tggcgtgcag cgccgagtcg tccgcgcggg cgtccttgcg
     7861 gtgccgggcc tcgctgcgca cccagtccac ggcccgccgg tgggccagca tggacagcca
     7921 ggtgcgcagc gagccgcggc gcggatcgaa ggcgtacggc ctgctccaca actgcgcgaa
     7981 gacctcctgc gccacatcct cggccgcggc cgggctgcgc gtgacccgga cggcgacccg
     8041 tcgcacgagc ccgccgtacg cggcatacgc ctcggccagc gcggtctcgt ccccgtacac
     8101 cagccgccga tgcagctcgg cgtccgtgag cgtcggatcc accggcacca ccacctcgtg
     8161 gtgccttcct agcgccctcc cggaccccgc gccagagggc gagcccctca gccggacagg
     8221 acgtccagca gccggtcgac atccgcgggc gtgttgtaca ggtggaacgc ggccctcaag
     8281 ttgcccgcac gatccgacac ttcgacaccg gcgcggctca agtcgggctg atgatgcccg
     8341 agttcgggta cggagacgat cgccgagccg ggcgcgggga tcggttcgtg gcccaaggcg
     8401 tgcagaccct cgcggaagcg gtcggcgaga gcgaggtcat gggcgtgtac gttcgccacg
     8461 cccagctcct cgatcagggc gagggagtgc cgggcaccgg cgtacgagaa gaggctcggg
     8521 ctctcgtcga accgccgtgc ggagtgggcg agttcctcga cggggccgta gcagctgtcc
     8581 cagggagcct cccccgcgac ccagcccgcg aacaccgggg tgagcccgcc gaggtcctcc
     8641 gggacgacca gaaaggccac gccccgcggg cagacgagcc acttgaaggc gacggaggag
     8701 acgtagtcgt acgcgtccgc ccgcaggggc agccatccgg cggcctggga cgcgtcgatg
     8761 tacgtacgtg ccccgtgcgc ccgcgcggcg tcgcggatcg cctccaggtc ggcgatccgc
     8821 ccatccgcgg actgcgcggc gctgaccgcg acgagcgccg tgcccgggcg tacggattcg
     8881 gccagccgct ccagcggtac ggcacggacc ttgagatcac cgcggacgtg gaagggggtc
     8941 acgacggagc tgaagtccgc ctcggcggtg aggacttcgg cgccctgcgg cagcgaggcg
     9001 gcgatcagtc cgctgtagac ggcgaccgag gccccggccg ccacccggtg tccgggaacc
     9061 ccgaccagcc gggcgaacga cgcgcgtgcg gcctcgacgt ccgcgaacat gtcggccggc
     9121 ctgccggcgg cgacggactc gacggcggtg cgcatggcgg ccaccgtgcg cgccggtacg
     9181 agtccggtgc tcgcggtgtt caggtaggtg ttctccgggg cgaactcggc acgtacgagg
     9241 ctctcgaagg tctccatggg accactctgc ggtcccatgg atcccccgtc cattgccgat
     9301 ttttacgtgg aaccgctaag aggcgcttat atgtgcgcgc cgacctgcgc ttttcagttc
     9361 tgcggaaccg cgcatccgtc cgggccgcag gcctccgcgt cggcggaacc gtcctggatc
     9421 agcttgagcg gcggccgctc gccccacgcc tgggtcagcg cctgcgcgaa gacctcggcg
     9481 ggctgggcac cggagacgcc gtacttgcgg tcgaggacga agaacggcac cccgttcgcg
     9541 ccgagctcgg ccgcctcacg ctcgtcggcg cgcacatcgt cggcgtacgc ggacgggtcg
     9601 gctatcacct tgcgggcggc gtcggcgtcg agcccggcgg cgacggccag ctccacgagc
     9661 cgttcgtcgt cgtcgaagac agtgcgctcc tcggcgaagt tcgccttgta gagcagaccg
     9721 atcagctcgt cctgcctgcc ctgctccttg gcgaagtgca gcaggcggtg catgtcgaag
     9781 gtgttgccgt ggtcgcggtc ccgggtgcgg tactccaggc cctcggcggc ggcctgcacg
     9841 cccaggttct cctcgccggc ctgcgcctgc gcctcgctca tgccgtactt cttggtgagc
     9901 atcttgatga ccggctggat gtcgcccttg gcacggcccg ggtcgagctc gaacgaacgg
     9961 tgcacgacct cgacctcgtc gcggtgcggg aaggcgtcga gcgccttctc gaagcgggcc
    10021 ttgcccacgt agcaccacgg gcaggcgatg tcgctccaga tctcgacgcg catgtcttcg
    10081 gctctctcca ggtcgtacga gtacggggta cgaggacacc ctccaaggac gtcgttgcgt
    10141 gaacgttcaa gcggctggtt tcattccccc cgtctccgtg acggtcgtcc ccccgtcacg
    10201 gagacggtca ttccccggtc acggagaagc gcaggtccag gtggtggtca ccctcggccg
    10261 gcgggttctc cggatcgggg atccagccga ggcgggcata gaaggcctgg gcgcgccggt
    10321 tgtcgacgtg cacgtcgagg acggcggtgc gcttgccgtc ggcctgccac tcctcgatgc
    10381 aggcggcgtg cagcgcggtg ccgat
//
