LOCUS       NZ_BMMM01000003        11041 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces albiflavescens strain CGMCC 4.7111 sequence03, whole
            genome shotgun sequence.
ACCESSION   NZ_BMMM01000003
VERSION     NZ_BMMM01000003.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces albiflavescens
  ORGANISM  Streptomyces albiflavescens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 654290)
  AUTHORS   Sun,Q. and Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 100X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014645835.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:24:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,828
            CDSs (total)                      :: 9,745
            Genes (coding)                    :: 9,386
            CDSs (with protein)               :: 9,386
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 359
            CDSs (without protein)            :: 359
            Pseudo Genes (ambiguous residues) :: 0 of 359
            Pseudo Genes (frameshifted)       :: 102 of 359
            Pseudo Genes (incomplete)         :: 321 of 359
            Pseudo Genes (internal stop)      :: 17 of 359
            Pseudo Genes (multiple problems)  :: 80 of 359
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:54:32
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 611337
            Orig. end                         :: 622378
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMMM01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11041
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="3"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11041
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11041
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6041](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6041
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(195..1070)
                     /locus_tag="IEY95_RS12160"
                     /old_locus_tag="GCM10011579_024630"
     CDS             complement(195..1070)
                     /NRPS_PKS="Domain: PKS_KR (4-190). E-value: 6e-10. Score:
                     31.3. Matches aSDomain:
                     nrpspksdomains_IEY95_RS12160_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012334.5"
                     /locus_tag="IEY95_RS12160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024630"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_229702845.1"
                     /transl_table=11
                     /translation="MEKLALVTGSTDGIGRGAAKLLAEQGWHVVVHGRDPRKVADALAD
                     VRKAARAAKGTAGEITSVTADLSRLEEVHALADDMLRTHGRLDALVNNAGVATPNRSAR
                     TRRVTPDGYQLEWQVNFLAAFALTLRLTGLLVASAPARVVNVSSVAQGWGAIHWDDLQL
                     ERRWDRMTAYAQSKLALTAFTNEYAHRLAGREVCANSLHPGMCSTKMVRSTFVFAPHRT
                     RYGAENITRLVTDPGLRSTSGAYFNERRRIEPNGFAHDPAGRRRLWDVALAQSGVADRA
                     DTVLPELLTA"
     aSDomain        complement(501..1058)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY95_RS12160_PKS_KR.1"
                     /evalue="6.00E-10"
                     /label="IEY95_RS12160_PKS_KR.1"
                     /locus_tag="IEY95_RS12160"
                     /protein_end="190"
                     /protein_start="4"
                     /score="31.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="ALVTGSTDGIGRGAAKLLAEQGWHVVVHGRDPRKVADALADVRKA
                     ARAAKGTAGEITSVTADLSRLEEVHALADDMLRTHGRLDALVNNAGVATPNRSARTRRV
                     TPDGYQLEWQVNFLAAFALTLRLTGLLVASAPARVVNVSSVAQGWGAIHWDDLQLERRW
                     DRMTAYAQSKLALTAFTNEYAHR"
     gene            1290..2288
                     /locus_tag="IEY95_RS12165"
                     /old_locus_tag="GCM10011579_024640"
     CDS             1290..2288
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073044.1"
                     /locus_tag="IEY95_RS12165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024640"
                     /product="phosphotriesterase"
                     /protein_id="WP_189185954.1"
                     /transl_table=11
                     /translation="MAKLDSLEPVIVETVEGGIPIEQLGTTLMHEHVFVLSSEIQENNP
                     QLWDEEAQIDSAVTRLTAAAERGVRTIVDLTVLGQGRYLPRIKKVAARVPLNIVVATGV
                     YTYNDEPMYFAFRGPGRVFEGPDPMVELFVRDITEGIGDTGVKAAVLKCATETEDLPEG
                     VERVLRATAQAHRVTGAPIQTHSNALVRNGLLQQKVFEEEGVDLSRVVIGHSGDTDDLG
                     YLRELLDAGSFLGMDRFGMEFIAPVEQRVKTVAELVHAGYAEQLVLSHDCPCYLDYLTP
                     DQRELVAPSWHYTHIHDDVLPALRHADVSDDAIHTMLVDNPRRFFSANAPY"
     gene            2304..2663
                     /locus_tag="IEY95_RS12170"
                     /old_locus_tag="GCM10011579_024650"
     CDS             2304..2663
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IEY95_RS12170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10011579_024650"
                     /product="hypothetical protein"
                     /protein_id="WP_189185955.1"
                     /transl_table=11
                     /translation="MSTQPTSPAGPWEELGAPLCLDTPLVKAWVEQVPPGGTRPLHTHR
                     KPWATVVLSGAKVRSLGADGEVIREGELPSGVVLFNPAEQPLCHQMHNVGETELVMVGL
                     QLEYGPPTEAPQEDP"
     gene            2660..3322
                     /locus_tag="IEY95_RS12175"
                     /old_locus_tag="GCM10011579_024660"
     CDS             2660..3322
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009995359.1"
                     /locus_tag="IEY95_RS12175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024660"
                     /product="HAD family phosphatase"
                     /protein_id="WP_189185956.1"
                     /sec_met_domain="HAD_2 (E-value: 4.2e-15, bitscore: 51.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIDTVVVDYGGVLTNPLVETFTAFAELAGIEVAALAEAFTAATH
                     RHGETPMAALEIGAITERQMAERILAELPPGSERVLDGHEFGELWFRGRKPNEPFVDFL
                     RQLRGDGYRIALLTNNVREWEPRWRAQLPVDELFTLVVDSHKEGVRKPDPKIYRILLDR
                     LDAEPGQCLFVDDTEENCLAARELGIESVRFTDTDTTVREVLALLEAGDPDPGGPAR"
     gene            3319..4950
                     /locus_tag="IEY95_RS12180"
                     /old_locus_tag="GCM10011579_024670"
     CDS             3319..4950
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003885.1"
                     /locus_tag="IEY95_RS12180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024670"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_189185957.1"
                     /transl_table=11
                     /translation="MTTEILIVGAGPTGLTTACTLLGLGVPCRIVDRREGPGEAPKALI
                     LWSGALECLDRIGVADKLVDTALPLAGATYWSRKKQIGGMRFGGLEGTAFRHPLCTPQP
                     VTEEALHARLLELGGTVEWQTEALGLHAEDDGVTVGLRPAQGRVEKETYRWLVGADGTH
                     SMVREAVGIPFEGHTYDRVFLLGDGPVEGPVPNDEAQYHITPDGVLVLVPLPEGGHRVF
                     FDIEPDGRTGSPSDQLLQELLDERGPGGLRLRGTWWSNRFRVHAKVAPRFRQGPVFLAG
                     DAAHAHSPAGGQGLNTGVQDGYDVGWKLAAVARGADPALLDSYETERRPASVQAVRNAD
                     QQTRMWLLRNPMARAMRDATMQSFSRRGVLEKQMLPQLAQLDLDHSASPAVADLPGASS
                     VPRAVRLGRRVPDTALVPVRGTRAKSLHTYLTAGRHSLIAYGDHAAGEIAVRALVALRS
                     RGVDDTANVLWIQPPDAASPAGQAGADGARCDTAHADGPGLGPASTPWLAYIRPDGVVG
                     ARSGISGVLELLERLPSRPAVTSDSA"
     misc_feature    complement(4994..5007)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.17"
                     /tool="antismash"
     gene            5001..6041
                     /locus_tag="IEY95_RS12185"
                     /old_locus_tag="GCM10011579_024680"
     CDS             5001..6041
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959546.1"
                     /locus_tag="IEY95_RS12185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024680"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_189185958.1"
                     /sec_met_domain="AfsA (E-value: 1.9e-18, bitscore: 61.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIPEARPTGISAPGEAPLTTEAVATAAATAGATLRYDATVSRTL
                     VHRTSVAEVFVTDSASTGENTFVVAAQLPRGHLIGENAPLYDFTLLVEVVRQAGVLVAH
                     RHLGVELGSAFIFRALNLRTVGLTQLRIGATPAHVLVSMTVHPRRNKAGRVQGFTFTGD
                     VSLDGEVGLAGDGALLIVSQRAYQALRAKRRAAAADHTLPRFNAAAPSSVGRREARNVV
                     ITEPVLAGPDNVSAQLVMDPSHPHMFDHPLDHVPGHLQMEAARQLGIASVARLHGLSPE
                     TLTIASLTTDFTDYTELDQVTELRARVDAFRRHEGLHTLAVPVAVDVQQDGAVTTSIRL
                     DVVQWT"
     gene            6032..7063
                     /locus_tag="IEY95_RS12190"
                     /old_locus_tag="GCM10011579_024690"
     CDS             6032..7063
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (12-222). E-value:
                     1.8e-16. Score: 52.0. Matches aSDomain:
                     nrpspksdomains_IEY95_RS12190_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.5"
                     /locus_tag="IEY95_RS12190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024690"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_189185959.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.9e-12, bitscore:
                     41.0, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2.3e-06,
                     bitscore: 22.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.8e-15, bitscore:
                     52.0, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLTTHRPRRVVVTGATGLLGSNTVRRLLDLGSEVTAVVRNPEPA
                     RSLLPDHKRLDLWQGDVLDVRSIARVLPGHDAVIHTAAYFREYYAPGGRDDALLTATNV
                     DAVGELLRAAADAGVPVVVHTSSIGTLGTRPDGRPSDEDTPEGRRTRENGYYASKLSSE
                     EVVRSFDARHGVRVPMVLPGWMWGPGDAGPTSAGRLFTSVARGELRAVPRAGNHVVDAR
                     DVADACVRAAVAGEHARRYIVAGTWVTLPEVCRLAARAAGVAPPREVPAHLALAVAAVL
                     EATARLRGRTPPATREGVRALLEGNRRRVSSSRAVSELGVTFRPLHRTLHDQADWHRAY
                     QHL"
     aSDomain        6068..6697
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IEY95_RS12190_NAD_binding_4.1"
                     /evalue="1.80E-16"
                     /label="IEY95_RS12190_NAD_binding_4.1"
                     /locus_tag="IEY95_RS12190"
                     /protein_end="222"
                     /protein_start="12"
                     /score="52.0"
                     /tool="antismash"
                     /translation="VTGATGLLGSNTVRRLLDLGSEVTAVVRNPEPARSLLPDHKRLDL
                     WQGDVLDVRSIARVLPGHDAVIHTAAYFREYYAPGGRDDALLTATNVDAVGELLRAAAD
                     AGVPVVVHTSSIGTLGTRPDGRPSDEDTPEGRRTRENGYYASKLSSEEVVRSFDARHGV
                     RVPMVLPGWMWGPGDAGPTSAGRLFTSVARGELRAVPRAGNHVVDAR"
     misc_feature    6200..6202
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7263..8900
                     /locus_tag="IEY95_RS12195"
                     /old_locus_tag="GCM10011579_024700"
     CDS             7263..8900
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018513458.1"
                     /locus_tag="IEY95_RS12195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024700"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_189185960.1"
                     /transl_table=11
                     /translation="MPEIGRRNLLTAATAVAAAKVAPSLLSQKAAATEPADAVGVCDPV
                     FGPVTVRPGDARYAELSVGVNKRWEAAPDSIQLVGSTEQVVSAVQDAVRAGKRVSIRSG
                     GHCYADFVSHRDVDVIIDTSGMNEVAYDAPRRAFSVQAGAQLGQIYEALYKGWGVTIPG
                     GACLTVGVGGHASGGGFGLLTRRFGLVADHIEAVEMVVVDHSGTVRRVIASRELSDPHH
                     DLWWATAGGGGGSFGIITRYWFRAAGSTGTDPALQLPRPPKEVLVSLLNVPWNQLDLQT
                     FGRLVHNFADWHVDNGSVSSPFISLSGYLLMRQGPAGGVALFTQVDATDPDADRLLADY
                     AAALTRDTGISTSFPSRRVPWLASTKLVGTSSPALLYDHTLRSAVKTAWMRRSFTDDQI
                     AVMHRNMTRTDYTNPNAAISLNGGGGRPNEVAIDANAFAHRDSAFIALYENFWVDPRED
                     GTHIGWLRDIYSQTFAATGGYPVPGDVTDGCYINDPDSDITDPAWNTSGVPWHTLYWKA
                     NYARLQQVKARWDPTDFFRHSQSIRLPA"
     gene            complement(9124..9591)
                     /locus_tag="IEY95_RS50150"
                     /old_locus_tag="GCM10011579_024710"
     CDS             complement(9124..9591)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987844.1"
                     /locus_tag="IEY95_RS50150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10011579_024710"
                     /product="hypothetical protein"
                     /protein_id="WP_268240449.1"
                     /transl_table=11
                     /translation="MSAPELIRIVSRDSPMALAQVERVRSELAALRPHIRTEVVPVKTT
                     GDKWMGDLAQVEGKGAFTKEVDAALLAGQADLAVYCVKDVPATGRPKGIEAVSGARDCG
                     RGGDATRMSTRWTGLLEAGAFTDGHGQRTTAVLAVLRDGKEDSERAVIATA"
     misc_feature    10461..10475
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.19"
                     /tool="antismash"
     misc_feature    10837..10851
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
ORIGIN
        1 cactgactcc ttgtcggcga agaactggta gaaggtgccc ggggagaccc cggcctcgcg
       61 ggcgatggcg gtggtgccgg gcacggtcgc cccggtacgg cacagcaggc gcgcgacggc
      121 atccagcaac tgctcgacac ggcgttcgcc tcgggcctgc cgcccgcccc gtctgggttc
      181 ggccaccggc ggggtcaggc ggtgagcagc tcgggcagca ccgtgtctgc gcggtcggcg
      241 acaccggact gggccagagc cacgtcccac agcctgcgtc ggccggccgg atcgtgggcg
      301 aagccgttgg gctcgatgcg gcgcctctcg ttgaagtagg cgccgctggt ggaccgcagt
      361 ccggggtcgg tgaccaggcg ggtgatgttc tcggcgccgt atcgggtgcg gtgcggggcg
      421 aagacgaacg tgctgcggac catcttggtg gagcacatgc ccggatgcag actgttggcg
      481 cacacctcgc ggccggccag gcggtgtgcg tactcgttgg tgaaggccgt cagggccagt
      541 ttggactggg cgtatgcggt catgcggtcc cagcgccgct ccagttgcag atcgtcccag
      601 tggatggcgc cccatccctg ggcgacggag ctcacgttga ccacacgggc gggcgcggag
      661 gcgacgagca gacctgtcag gcgcagggtg agggcgaagg cggcgaggaa gttcacctgc
      721 cattcgagct ggtagccgtc cggggtgacg cgacgggtgc gggcgctgcg gttgggtgtc
      781 gccacgccgg cgttgttgac cagggcatcc agtcggccgt gcgtgcgcag catgtcgtcg
      841 gcgagggcgt gcacctcctc caggcgggag aggtcggcgg tcaccgaagt gatctcgccg
      901 gcagtgccct tggcggccct ggccgccttg cgtacatcgg ccagcgcgtc ggccactttg
      961 cgggggtcgc ggccgtgcac gacgacgtgc cagccctgtt cggccagcag cttggcggcc
     1021 ccgcgcccga tcccgtcggt gctgccggtc accagggcga gcttttccat gtgcgccacg
     1081 tgcgctcctc acagagatga acgtcatgca gaattaacat tcggcgagca cattcccaat
     1141 ccctcacttt ctctgctgga ttcgccgctg gcaaggtaac tgtcagcact attggtaatg
     1201 ccaccaccat gccgtcagat actcgttgac aaaccaactt ggggatcgag accgtagcag
     1261 cggagcagac aactagatca gggggataaa tggcgaagtt ggattccctg gaacccgtca
     1321 ttgtcgaaac ggtggagggt ggaattccga ttgaacagct cggaacaact ctgatgcatg
     1381 agcacgtctt tgtgctcagc tcggagatac aggagaacaa tcctcaactc tgggacgagg
     1441 aggcgcagat cgacagtgcc gtcacccggc tgaccgccgc tgccgagcgt ggggtgcgga
     1501 cgatcgtcga cctcaccgtc ctcggccagg ggcgctatct gccccgtatc aagaaggtgg
     1561 cggcccgggt accgctcaac attgtcgtgg ccaccggcgt ctacacctac aacgacgaac
     1621 cgatgtactt cgccttccgc ggccccggcc gggtcttcga gggacccgac ccgatggtgg
     1681 agctgttcgt gcgcgacatc accgagggca tcggtgacac gggggtcaag gcggccgttc
     1741 tcaagtgcgc gaccgagacc gaggacctgc ccgagggcgt ggagcgcgtg ctgcgcgcca
     1801 ccgcccaggc gcatcgcgtg accggcgcgc cgatccagac gcacagcaac gccctggtgc
     1861 gcaacgggct gctgcagcag aaggtcttcg aggaggaggg cgtcgacctc agccgggtcg
     1921 tcatcggcca ttccggcgac accgacgacc tcggctatct gcgcgaactg ctcgatgccg
     1981 gctcgtttct cggcatggac cgcttcggaa tggagttcat cgcccccgtc gaacagcgcg
     2041 tgaagaccgt ggccgaactg gtgcacgccg gctacgccga gcaactggtg ctgtcccacg
     2101 actgcccgtg ttacctggac tacctcacac cggaccagcg cgaactcgtc gccccctcct
     2161 ggcactacac gcacatccac gacgacgtgc tgcccgcgct gcgccatgcg gacgtcagtg
     2221 acgacgcgat ccacaccatg ctcgtcgaca atccccgccg ctttttctcc gccaacgccc
     2281 cctactgacc tctcggagac tccatgtcca cacaacccac cagcccggcg gggccctggg
     2341 aggagctcgg cgcaccgctg tgcctcgaca cccccctggt gaaggcctgg gtcgaacagg
     2401 tgccccccgg cggcacccgc ccgctgcaca cccaccgcaa gccctgggcg acggtggtgc
     2461 tcagcggagc caaggtccgt tcgctcggcg ccgacggcga ggtcatccgc gagggcgaac
     2521 tccccagcgg cgtggtgctg ttcaacccgg cggaacagcc gctgtgccac cagatgcaca
     2581 acgtcggcga aaccgaactg gtcatggtcg gtctgcaact ggagtacgga ccgcccaccg
     2641 aggccccaca ggaggacccg tgaccatcga caccgtcgtc gtcgactacg gcggcgtgct
     2701 caccaacccc ctggtcgaga ccttcaccgc cttcgccgag ctggcgggga tcgaggtcgc
     2761 ggccctcgcc gaggcgttca cggccgccac ccacaggcac ggcgagaccc ccatggccgc
     2821 cctggagatc ggcgccatca ccgagcggca gatggcggag cgcattctgg ccgagctgcc
     2881 cccgggatcc gaacgcgtcc tggacggcca cgagttcgga gaactgtggt ttcgcggccg
     2941 gaagcccaac gagcccttcg tcgacttcct gcgccaactg cgcggcgacg gctaccggat
     3001 cgccctgctc accaacaacg tccgcgagtg ggagccgcgc tggcgcgccc aactgccggt
     3061 cgacgagctg ttcacactcg tcgtggactc gcacaaggag ggcgtgcgca agcccgaccc
     3121 gaagatctac cggattctgc tcgaccggct cgacgcggag cccgggcagt gcctgttcgt
     3181 cgacgacacc gaggagaact gcctcgccgc ccgcgaactc ggcatcgaat ccgtccggtt
     3241 caccgacacc gacaccaccg tccgcgaggt gctcgccctc ctcgaggccg gcgacccgga
     3301 cccgggaggt cctgcgcgat gaccacggag attctcatcg tcggagccgg ccccaccggc
     3361 ctgaccaccg cctgcaccct gctcggcctc ggcgtgccct gccggatcgt cgaccggcgt
     3421 gagggcccgg gtgaggcgcc caaggcgctg atcctgtgga gcggtgcgct ggagtgcctg
     3481 gaccgcatcg gcgtcgccga caaactcgtc gacaccgcgc ttccgctggc cggcgccacc
     3541 tactggtccc gcaagaagca gatcggcggc atgcgcttcg gcggtttgga gggcaccgcc
     3601 ttccgtcacc cgctgtgcac cccgcagccc gtcaccgagg aagcgctgca cgcccgcctc
     3661 ctcgaactcg gtggcacggt ggagtggcag accgaggccc tcggcctcca tgccgaggac
     3721 gacggcgtca cggttggcct gcgtcccgcc caggggcggg tcgagaagga gacctaccgt
     3781 tggctggtcg gtgccgacgg cacgcacagc atggtgcgcg aggcggtcgg catccccttc
     3841 gaaggccaca cctacgaccg ggtgtttctg ctcggcgacg gcccggtgga gggccccgtc
     3901 ccgaacgacg aggcgcagta ccacatcact ccggacgggg tgctggtcct ggtgcccctg
     3961 ccggagggcg gacaccgggt cttcttcgac atcgagcccg acgggcgcac cggatccccc
     4021 tccgaccagc tgctgcagga gttgctcgac gagcgcggtc cggggggcct gaggctgcgc
     4081 ggcacctggt ggtccaaccg cttccgggtg cacgccaagg tcgccccgcg cttccgccag
     4141 ggcccggtct tcctggccgg cgacgccgcc cacgcgcaca gtcccgcggg cggccagggc
     4201 ctcaacaccg gtgtgcagga cggctacgac gtcggttgga agctcgccgc cgtcgcccgt
     4261 ggcgccgacc cggccctgct cgacagctac gagacagagc gtcgtcccgc ctcggtgcag
     4321 gcggtgcgca acgccgacca gcagacccgg atgtggctgc tgcgcaaccc catggcccgc
     4381 gccatgcggg acgccaccat gcagtccttc agccgccgcg gcgtgctgga gaagcagatg
     4441 ttgccgcagc tcgcccagct cgacctcgac cactcggcca gccccgccgt ggccgacctg
     4501 cccggcgctt cctccgtgcc ccgcgcggtc cggctgggca ggcgcgtacc ggacaccgca
     4561 ctggtcccag tgcggggcac gcgggcaaag tcactgcaca cgtacctgac cgcggggcgg
     4621 cacagcctga tcgcgtacgg ggaccatgcg gccggcgaga tcgccgtgcg ggccctggtc
     4681 gccctgcgca gccggggcgt ggacgacacg gcgaacgtgc tgtggatcca gccccccgac
     4741 gccgcttccc cggccgggca ggccggcgcc gacggtgccc gctgcgacac cgcgcacgcg
     4801 gacggccccg gtctcggccc ggcgagcacg ccgtggctgg catacatccg cccggacggc
     4861 gtcgtggggg cgcggtccgg gatctcggga gtcctcgagc tgctggaacg cctgccctcg
     4921 cggccggcgg tgacgtccga ctcagcttag gaccccctgg gctcacttct caggaacctt
     4981 ggaagggatc cgcttcgatc atgtccatac ccgaagcacg acccaccggg atcagcgcgc
     5041 cgggggaagc gccgctcacg accgaggccg tggccaccgc cgcggccacg gccggtgcga
     5101 cgctgcgtta cgacgcgacg gtctcccgca ccctggtcca ccgcacctcc gtcgccgagg
     5161 tgttcgtcac cgactccgcc tcgaccggcg agaacacgtt cgtcgtggcc gcgcagttgc
     5221 cgcgcggcca cctgatcggc gagaacgcgc cgctgtacga cttcaccctg ctggtggagg
     5281 tggtacggca ggcgggcgtc ctggtggccc accgtcacct gggcgtggag ctcggctcgg
     5341 cattcatctt cagagccctc aacctgcgta cggtgggcct gacccagctg cggatcggcg
     5401 ccacgccggc ccatgtgctg gtgtcgatga ccgtgcaccc ccgtcgcaac aaggcagggc
     5461 gcgtccaggg attcacgttc accggtgacg tctcgctcga cggagaggtc gggctggccg
     5521 gggacggtgc gttgctgatc gtgtcccagc gcgcgtacca ggccctgcgg gccaagcgac
     5581 gggcggccgc cgcggaccac acgctgccgc ggttcaacgc tgccgcgccg tcctcggtgg
     5641 gccgccgcga ggcccgcaac gtggtcatca ccgagcccgt cctggccgga ccagacaacg
     5701 tctcggccca gctggtgatg gatccgagcc atccgcacat gttcgaccac ccactggacc
     5761 atgtgcccgg tcatctgcag atggaggcgg cccgccagct gggcatcgcc tccgtcgcac
     5821 ggttgcacgg gctgtctccg gagaccctca ccatcgcgtc cctgaccacc gacttcaccg
     5881 actacacgga gctggaccag gtgaccgaac tgcgcgcgag ggtggacgcc ttccgccgcc
     5941 acgaggggct gcacaccctc gccgtcccgg tcgccgtcga cgtgcagcag gacggggcgg
     6001 tgacgacgtc gatccggctg gacgtggtcc agtggacctg accacccatc gaccccggcg
     6061 ggtcgtcgtc accggcgcga ccggtctgct cgggagcaac accgtacggc gcctgctcga
     6121 cctgggcagc gaggtgaccg ctgtggtccg aaaccctgaa ccggcccgct ccctgctccc
     6181 ggaccacaag cggctggact tatggcaggg tgacgtgctc gacgtacgaa gcatcgcccg
     6241 cgtgctccca ggacacgacg cggtgatcca caccgcggcg tacttccggg agtactacgc
     6301 ccccggcggc cgcgacgacg cgttgctgac cgcgacgaac gtggacgcgg tgggcgaact
     6361 gctacgggcg gcagccgacg ccggggtgcc ggtggtggtg cacaccagct ccatcggcac
     6421 gctgggaacc cgccccgacg ggcggccctc cgacgaggac accccggaag gccgccggac
     6481 ccgggagaac ggctactacg ccagcaagct gagctccgag gaggtcgtac gctccttcga
     6541 tgcccggcac ggggtgcggg tgcccatggt gctgccgggg tggatgtggg gcccgggaga
     6601 cgccgggccc acgtccgccg ggcggctgtt cacctccgtg gcacgagggg agctgcgcgc
     6661 ggtaccccgg gccggcaacc atgtcgtgga cgcccgcgac gtcgccgacg cgtgcgtacg
     6721 cgccgccgtg gcgggcgaac acgctcggcg ttacatcgtc gccgggacct gggtgacgct
     6781 gccggaggtg tgccggctcg ccgcccgcgc cgcgggggtg gcgcccccgc gcgaggtccc
     6841 ggcccatctc gcgctcgccg tcgccgccgt cctcgaggcc actgcacgcc tcaggggccg
     6901 tactcctccc gcaacgcggg agggcgtacg cgcccttctg gagggcaacc gccggcgcgt
     6961 gagctcctcg cgtgccgtca gtgaactggg cgtcaccttc cgcccgctgc accgcaccct
     7021 gcacgaccag gcggactggc accgcgccta ccagcacctg tgagcggcgg gtgccgccgg
     7081 gagccctcaa cccggcggcg cccgcccttc ccccagcccg gtggcagcgc cacccgtgaa
     7141 atttccccgg gtcggctgga tactcttcct cgcattcccg ttcatctcat tctcgttcac
     7201 cacgttcccg ttcctcgcat tccctttgcg gaagtcccag gaagtccagg ggagatacag
     7261 caatgcccga aatcggcaga cgaaaccttc tgaccgcggc cacggccgtg gccgcggcga
     7321 aggtcgcccc gtccctcctc tcacagaaag ccgcggcaac ggaacccgcg gacgccgtgg
     7381 gcgtatgcga cccggtgttc ggcccggtga ccgtgcgccc aggtgacgcc cggtacgccg
     7441 aattgtccgt cggcgtcaac aaacgatggg aggccgcacc tgactccatc cagttggtcg
     7501 gttcaacgga acaggtggtg tcggcggtac aggacgccgt gcgcgccgga aagcgggttt
     7561 ccatccgcag cggcgggcac tgctacgcgg atttcgtgag ccatcgggac gtggacgtca
     7621 tcatcgacac ctccgggatg aacgaggtgg cgtacgacgc cccacgccgg gccttctcgg
     7681 tgcaggccgg agcccaactg ggccagatct acgaggccct gtacaagggc tggggcgtga
     7741 cgatccccgg cggcgcctgc ctgaccgtcg gcgtcggcgg gcatgccagc ggcggcggct
     7801 tcggattgct gacccgaaga ttcgggctgg tcgccgacca catcgaggcc gtggagatgg
     7861 tggtcgtgga ccactccggc accgtacgac gcgtgattgc ctcacgcgag ctgtccgatc
     7921 cgcaccacga tctgtggtgg gccaccgcgg gcggtggagg cggcagcttc ggcatcatca
     7981 cccgctactg gttccgcgcg gccggatcca ccggcaccga tccggccctg caactccccc
     8041 ggccgcccaa ggaggtgctg gtcagcctgc tcaacgtccc ctggaaccag ctggatctcc
     8101 agaccttcgg ccgactggtc cacaacttcg ccgactggca cgtggacaac ggctcagtgt
     8161 cctcaccctt catctccctc tccggctacc tgctgatgcg ccagggtccg gccggcggcg
     8221 tcgccctgtt cacccaggtc gacgcaaccg acccggatgc cgaccggctg ctcgccgact
     8281 acgcggccgc cctcacccgc gacaccggca tctccacctc gttcccctcc cggcgggtgc
     8341 cgtggctggc ctccaccaag ctcgtcggca ccagtagccc ggccttgctg tacgaccaca
     8401 ccctgcgcag tgccgtcaag acggcctgga tgcggcggag tttcaccgac gaccagatcg
     8461 cggtcatgca ccgaaacatg acgcgcacgg actacaccaa tcccaatgcg gcgatatccc
     8521 tcaacggcgg tggcggccgg cccaatgagg tagctatcga cgcaaacgcg ttcgcgcacc
     8581 gcgattccgc cttcatcgcg ctgtacgaga atttctgggt cgacccgcgc gaggacggga
     8641 cccatatcgg ctggctgcgc gacatctaca gccagacgtt cgcggcaacc ggcggatatc
     8701 cggtgcccgg cgacgtcacc gacggctgct acatcaacga cccggattcc gacatcaccg
     8761 accccgcgtg gaacacctcc ggcgttccct ggcacacgct gtactggaag gcgaactacg
     8821 cgcgcctgca acaggtgaag gcccgctggg atcccaccga tttcttccgg cactcgcagt
     8881 cgataaggct tccggcctga tccaccgccc gatgacgtcc gcctctcggc cgaccgtgac
     8941 ctgccggcgc agggaccaca cggcccgctt ccacgtcgac caggacggtg tcggcgaccg
     9001 cctgcggccg cgcgactccg accacgacgg ccacaccgac ctcttcctgg acaacctgcg
     9061 ccggcccggc acctcgggcc ggcatcacga cgtcgggcgt ccaggaagcc gtctcctccg
     9121 acttcatgca gtagcaatga ctgcgcgctc cgagtcctcc ttgccgtccc gcagcaccgc
     9181 aaggacggct gtggtgcgct gaccgtggcc gtcggtgaag gcgccggcct ccaacagtcc
     9241 agtccaccgg gtgctcatgc gggtggcgtc accgccgcgg ccgcagtcac gggctccgct
     9301 tactgcctcg atccctttgg gacggcccgt tgcggggacg tccttgacgc agtacaccgc
     9361 caggtccgcc tggccggcga ggagggcagc gtcgacttcc ttggtgaacg cacccttgcc
     9421 ctcgacctgg gccaggtctc ccatccactt gtcgccggtc gtcttgaccg gcacgacctc
     9481 ggtgcggatg tgcgggcgga gcgcggcgag ttcgctgcgc acgcgctcga cctgagcaag
     9541 cgccatgggc gagtcgcggg agacgatgcg gatcagttca ggggcggaca tgccgcccac
     9601 cctagccctt tacgggcggt caccgagcag cagccccaag ccccatctca tcggcacagc
     9661 acacaagttg gagacctcgc acggatgcgc gagaacgatg tcgacgcagt gctggcgacc
     9721 gagcagccga ctggggacgg actcccgcgc gaggaggctg tccgtgccat cgcaggagga
     9781 gtcgacgtct gttctcactg cggcccgaca gcgaactcgg gattctggac tgactcgact
     9841 gaccacgccc ctacactgac gcgcctcgag tacagcctgg ggggtgcttc tatctgttcg
     9901 gtcaaggtct gtgggtttgg tcaggctgtt tgttcgtgcc ctggttggta aagggtgcgg
     9961 tgcttgagca tggcgtgcag gacgtcgacg cggcggcggg tgaggcagat gagggcggcg
    10021 ttgtggcgct tcttttccgc gcgtttcctg tcgtagtagg cccgggattc ggggctgctc
    10081 agtgaggcga acgaggccag gaacagggcc cgttttgagc atcttgttgc cgcctcgggg
    10141 cggtccttcg ccccggatgg aggttcccga tcgacgggtg gtgggggtca gtcccgcgta
    10201 ggaggccagg tgggcggcgg tggggaacgc gctggcgtcg ccgacctcgg ccaggatgcg
    10261 ggcgccggtc ctgaccgcga ccccgggcat ggaggtcagg accttcgcga gagggtgggc
    10321 ctccagcagt gcggcgaccc gggtctccag gatgtcccgg cgcttgagga ggaccgagag
    10381 ggcttcggcc agaccggaga tgatttcccc tgccgaggcc gtgccgggga cctgcacggt
    10441 ctgctcggcc agggcgttgg tgatgtggtc ggccagggtg cgggcactgc gcatccgcgg
    10501 ggcggcctgg agcatgacct gcgtgatctg ctcggtgccg gcctgggcca gctgggccgg
    10561 ggagccgaag gtctgcagca gggccaggac ggccgggtgc cgcaggcggg gcccgaggac
    10621 gcgttcgagg gcggggtgga tggaggtgag cagtccgcgc agccggttgg aggtgcgggt
    10681 ggcgtcctgg gcgaggtcgt cgtcgtggcc aaggatcatg gttcagttcg gcccgcacgc
    10741 tctcgtcggg gtcgatggag tgcagcaggt ggggcatggt gcgggcggcg tcggcgatga
    10801 cgtaggcatc gcgggcgtcg gtcttgccgg tgccggggtg cagatcggcc agccggcgca
    10861 tcgacaggcc cgggagatag gcgacttcgc atccacaagc gcgggcgacg gtcaccgcca
    10921 gggtgccgat ggaggccggc tggtcgacca cgaccaggac cttccccttg cgcttgaggc
    10981 gggcgaacag ctcccgcagc tggggctcgc cgttgggtag cggcttgtcg aaggtcgtac
    11041 g
//
