LOCUS       NZ_CP061033            10318 bp    DNA     circular CON 27-APR-2024
DEFINITION  Corynebacterium zhongnanshanii strain zg-320 chromosome, complete
            genome.
ACCESSION   NZ_CP061033
VERSION     NZ_CP061033.1
KEYWORDS    RefSeq.
SOURCE      Corynebacterium zhongnanshanii
  ORGANISM  Corynebacterium zhongnanshanii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Corynebacteriaceae; Corynebacterium.
REFERENCE   1  (bases 1 to 2206897)
  AUTHORS   Zhang,G.
  TITLE     novel species in genus Corynebacterium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206897)
  AUTHORS   Zhang,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2020) Institute for Prevention and Control of
            Infectious Diseases, China Cdc, 155 Changbai Road, Changping
            District, Beijing, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1910.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014490575.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 03:58:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,980
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,906
            CDSs (with protein)               :: 1,906
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 2 of 14
            Pseudo Genes (multiple problems)  :: 2 of 14
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:15:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1260063
            Orig. end                         :: 1270381
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP061033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10318
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10318
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10318
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5318](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5318)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     tRNA            complement(873..948)
                     /anticodon=(pos:complement(1260975..1260977),aa:Glu,
                     seq:ctc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IAU67_RS05650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="IAU67_05650"
                     /product="tRNA-Glu"
     gene            complement(873..948)
                     /locus_tag="IAU67_RS05650"
                     /old_locus_tag="IAU67_05650"
     gene            1156..1782
                     /locus_tag="IAU67_RS05655"
                     /old_locus_tag="IAU67_05655"
     CDS             1156..1782
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /locus_tag="IAU67_RS05655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05655"
                     /product="lipoprotein LpqH"
                     /protein_id="WP_151841737.1"
                     /transl_table=11
                     /translation="MNKQVRMLIAALFAIIVLISLVVVFKFTGDSADDTASSSASQSSP
                     SSDASAPPTASDTPEDPAHPADPNAEDAPRPEDNPEEGKPPAAPAAAPFVGQLDNQPFD
                     VVNPRIECHSEAQFTQITATGQGDQPGRFAAAQFNGNDRLMSVSLGDGATSRGYVVETA
                     SNQGHATLERIGDKTYRIAGDALEVDYKTMNEAGTKPFSFDITCP"
     misc_feature    complement(1190..1205)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 24.13"
                     /tool="antismash"
     gene            1831..2340
                     /locus_tag="IAU67_RS05660"
                     /old_locus_tag="IAU67_05660"
     CDS             1831..2340
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017309.4"
                     /locus_tag="IAU67_RS05660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05660"
                     /product="lipoprotein LpqH"
                     /protein_id="WP_151841738.1"
                     /transl_table=11
                     /translation="MTTHISPTFAKRLYGALGASVLLISLGACGREEARAVMYTATLDG
                     EQFQTDDPTVTCTDNGGLKEITVGHTQQDGGEDAAPTNYITALINPDGTFVEDITISDN
                     STFNFTYSPYRKIGSASAGESDGTYSIKGEGQMTRLDNQDQDTQDQDAPEKLVPFELEL
                     TCPSEH"
     gene            complement(2405..3073)
                     /locus_tag="IAU67_RS05665"
                     /old_locus_tag="IAU67_05665"
     CDS             complement(2405..3073)
                     /GO_function="GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /locus_tag="IAU67_RS05665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05665"
                     /product="copper chaperone PCu(A)C"
                     /protein_id="WP_187767864.1"
                     /transl_table=11
                     /translation="MKKSAFAVVSVLALGGSLAACSSDDAEEAKNSASSAASSATSAAS
                     SAMSSASESAKSSTGLTFENGFCKAKPSADKAKDGEMADMTACFGTLVNNTDKDITITG
                     FSIEDMKDEGATFELHEVVDGKMQKKEGGFEIKAGESKELKPGGEHLMVLNLRDDVEPG
                     DDLTIVFETKDGETFKTEVEGREIGAGNESYGSDDHDHDGHDHDHHDHDGHDHDHHDHN
                     "
     misc_feature    complement(3025..3040)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.24"
                     /tool="antismash"
     gene            complement(3294..4472)
                     /locus_tag="IAU67_RS05670"
                     /old_locus_tag="IAU67_05670"
     CDS             complement(3294..4472)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="IAU67_RS05670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05670"
                     /product="MFS transporter"
                     /protein_id="WP_187767865.1"
                     /transl_table=11
                     /translation="MSTQRSFGLALLGLFLLAANLRMALTSVAPVVSDIQADLGLTGAQ
                     VSIVTSLPLLVFAIVSPLVPRLLVRWGIERTLIAGTGLLAVGLIVRSLPAAALLWVGTG
                     FIGLAIATLNVTLPALVKRDFPDRIGFVTGTYAAVQAGFAAIAAGLAVPISHASGWGEA
                     GWRLATSVWCVMALAAFAHLIIQGRRAGREAGVGTVPSSSFNPWKHPLAWAITGFMGFQ
                     SAMFYVIVAWMSSIEQSMGISAGAAGVHQAFMNIGSLVGSFSCSVLLSRLRGQRAIVVA
                     IAVMMGVPLIILMHAPQIAGLLIFVEGIACGLAFALSLSLFALRSRKPETAAALSGMGQ
                     GMGYVIAASGPILLGAVHDATGQWTSGLWIMVAVSVLCLLCGLAAAKDTTVD"
     gene            4569..5009
                     /locus_tag="IAU67_RS05675"
                     /old_locus_tag="IAU67_05675"
     CDS             4569..5009
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005290392.1"
                     /locus_tag="IAU67_RS05675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05675"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_151841741.1"
                     /transl_table=11
                     /translation="MSDSQSSAAHNSDHTEDGRYDAGIRVRREVMGDDFVDAALERAAG
                     SDGEELQKHVTATVWGSVWTRDGLSKRDRSLLNIGMLVALRATQELRGHIKGGLANGLT
                     REEITEAIIHSSGYCGAPAALSAMKVAQEVLEAELGPKDPSA"
     gene            complement(5001..5318)
                     /locus_tag="IAU67_RS05680"
                     /old_locus_tag="IAU67_05680"
     CDS             complement(5001..5318)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="IAU67_RS05680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05680"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_151841742.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 6.7e-07,
                     bitscore: 26.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKKTLWAGLAGAALLLGGGGYLVLGGSEQGAQTVYPHEGGEWD
                     FGSSGGRTWSNFKHDAPHSASVRGHQFVDSGCVAGDSWARAEAPSRWLSALGDEQNKSL
                     C"
     gene            complement(5315..5968)
                     /locus_tag="IAU67_RS05685"
                     /old_locus_tag="IAU67_05685"
     CDS             complement(5315..5968)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004600259.1"
                     /locus_tag="IAU67_RS05685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05685"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_225723453.1"
                     /transl_table=11
                     /translation="MTVMVSVKNVSKAVGEKKPRRRLWEKLSFDVSSGELVSVTGPSGC
                     GKSTLLNCLGLLDRPDNGDIDIKGIPLASASARQRMRMRRESVGYLFQDYALIDNDTVR
                     QNIALGSRARGRQLTEDIAQALAEVGLEGYENHRVYQLSGGEQQRVAIARLSVRKPEVI
                     LADEPTASLDRSNAASVLTHLRTLSDHGAAVIVVSHDPWVIAQTDRTIELGENS"
     misc_feature    5942..5955
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.37"
                     /tool="antismash"
     gene            complement(5977..7887)
                     /locus_tag="IAU67_RS05690"
                     /old_locus_tag="IAU67_05690"
     CDS             complement(5977..7887)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IAU67_RS05690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="IAU67_05690"
                     /product="hypothetical protein"
                     /protein_id="WP_151841744.1"
                     /transl_table=11
                     /translation="MQIQTFAPHDQGGDSSADSLVRDLGRIVTEHDATILVEIPTLEGR
                     VAYAAGDKAESWIHKGYQGLPGTPPLDVRPLEDLPHGDYRQMFEVIGGDDVLQELVSYL
                     DARGVTYHIPENQQWMYLLGGTALGKMAALMIGIGFVLVFVGIVLNSHTDAVRRLHGIG
                     LLSRAKAELSAARGTLLTATSAAVVLVDVLLWWYSNTASAYQLVLFQAAFIGIALVACI
                     FALFVGLFLLKIAPVVQLLKGKLPAKSVLVSMFAVRIGACIAVASLSIGALNYTTEWNE
                     QKDEVDGWRNSPSAYGLTLSGARKLEDLHEASGRLASHMRDISSQNRLLYAQFRDSGMT
                     PGSHLDRDIMVYNTTAAENSLSGEVSRSFGEQPRDKQPLVLVPDSLPAAVDSHELLAPI
                     VGEARVHTAVYRAGDSRARTWEVGLDEWMNRAETHDPIVVVMPNDLIGLSDRNLVASVT
                     QHDVLLSSYDDFQRLHDDREVGSFVRAAVPMSQTWSQHHQAMGRILWVYVGGFVATVLL
                     CCIASLAVWLSFMKVFHQRLRAAYLHARWPIRPLLTALGAELIVCACVLYFLFTRAATA
                     RAWEAGGSAEGAADPALIALFHVPSSAWWITLALFVSTSIPASGLVLGRRTQRSLVVTR
                     R"
     misc_feature    complement(8180..8197)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.32"
                     /tool="antismash"
     tRNA            complement(8245..8317)
                     /anticodon=(pos:complement(1268344..1268346),aa:Glu,
                     seq:ctc)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IAU67_RS05695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="IAU67_05695"
                     /product="tRNA-Glu"
     gene            complement(8245..8317)
                     /locus_tag="IAU67_RS05695"
                     /old_locus_tag="IAU67_05695"
     tRNA            complement(8319..8390)
                     /anticodon=(pos:complement(1268418..1268420),aa:Gln,
                     seq:ctg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IAU67_RS05700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="IAU67_05700"
                     /product="tRNA-Gln"
     gene            complement(8319..8390)
                     /locus_tag="IAU67_RS05700"
                     /old_locus_tag="IAU67_05700"
     gene            complement(8569..10059)
                     /gene="gltX"
                     /locus_tag="IAU67_RS05705"
                     /old_locus_tag="IAU67_05705"
     CDS             complement(8569..10059)
                     /EC_number="6.1.1.17"
                     /GO_component="GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA]"
                     /GO_function="GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="gltX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320056.1"
                     /locus_tag="IAU67_RS05705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IAU67_05705"
                     /product="glutamate--tRNA ligase"
                     /protein_id="WP_151841745.1"
                     /transl_table=11
                     /translation="MSDVRVRFCPSPTGTPHVGMVRTALFNWAYARHTGGKLIFRIEDT
                     DAARDSEESYQAIIDSLKWLNLGWDEGIEVGGPHEPYRQSRRMDIYAEVLDKLKASGDV
                     YPAYSTAAEVEERHKAAGRDPKLGYDNFDRDLTEEQIAAFEAEGREPVWRLRMPDNRTY
                     TWTDLVRGEMNVDGKTVPDFVVARSNGQPLYTLVNPVDDALMGITHVLRGEDLLPSTPR
                     QIALYEALVRIGVAQQVPTFGHLPFVMGEGNKKLSKRDPESNLFNHRDNGIIPEGMLNY
                     LSLLGWSLSADEDIFSMQQLIDNFDVADVKPNPARFDQKKLEAINADHIRLLDLEDFTT
                     RLREYLETYKDFPADYPADKFAFAAELVQTRIKMLGDAHGLLKFLVTADEDLVLDEKSA
                     KKNLKEDAVEVLQVAIAQLEGLSEEEFRTENIEHALQSKLIEEMELKPRKAYGALRVAI
                     SGAAVSPPLFESMELLGKESTLARLKAAQAQTPYVAEQ"
ORIGIN
        1 ttaccggctg cagtcacctg accggtgaac aggcggccga aggtgttggc agcctcaatc
       61 acggcgcggt agccggcgat gttcgcctgg gagctcagca cgtccatcga ctgcgcacgg
      121 ctgatacgcg gcacggtgtc catggcgatg gaggtgacgt ttttgtccgc gaactcctgg
      181 atcagctgct cgttgcgcgc aggagcaaga cgggcgatga gagccgcgcc tggcttcaga
      241 tctgcccgct gctctgcggt gggctcgtcg agagcggtaa taatgtccga ctgccacact
      301 tcttcgccga cgatacgcgc gccggcttct tcgtactgag agtcggggta gttggaatcg
      361 tcgcccgctc cagattgaac ggcaacgtcg tagccaagct tgatgagctt gcctacggtg
      421 tctggggtgg ccgaaaccag ggaggctggt tctctcggaa taccaataag caagggaagg
      481 ctcctctagt tcacacaaca cccgtgctcc ccgtgcggcg cgcctctatg tgatggcgcg
      541 cgacagcata ggcagcaggg cacgttgtgc gtgagtgatc tgtccccgca cgcacgccag
      601 cctgtgcccg ttcggggcct ggctctggcg tgactgcgag cacaattagg tcagaatgta
      661 ggtagttcta aaactatagg agaggcgcga gaatgtgtac cgcgatttga gaaaactacc
      721 cccgtttatc ccaatatccg agaatgtcca gcgtatggcc cctaccctat ggggtaacgc
      781 ccgggggtgg tttcccacac cccctgcgcg gaaatgccac acccgctgat acaccacaac
      841 cgccgacaca tggtcggcgg ttgctgatcg attggtaccc cgtacgggat ttgaacccgt
      901 gttaccggcg tgagaggccg gcgtcctagg ccgctagacg aacggggcgt tcacactgtc
      961 cgagcacttc ttctcgaagc gctccactgt gcagtgttct ctaagaactg ttctctagga
     1021 actgttcgct cggaactcca aaaactttac acaccctccc cacctttacc taatccccag
     1081 gtcacaggcc gtatcctgcc gccgctcccc atcggcattc aaccgctcgc ccataattgg
     1141 ctaagatggt ctgctatgaa taaacaggtc cgaatgctga tcgctgcctt gttcgccatc
     1201 atcgtactca tcagcctcgt cgtggtgttc aagttcacgg gcgacagtgc tgacgatact
     1261 gcgtcaagct ctgcgtccca gtcctcccct tcctccgacg ccagcgcgcc acccaccgcc
     1321 tcagataccc ccgaggatcc cgcgcacccg gccgacccca acgccgagga cgcaccccgt
     1381 cccgaggata accctgagga aggaaagccc ccagccgctc ccgcggctgc accgtttgta
     1441 ggccagctgg ataaccaacc cttcgacgtg gtgaacccac gcatcgaatg ccactcggaa
     1501 gcacagttca cgcaaatcac cgccaccggc cagggcgacc agccgggccg cttcgccgcg
     1561 gcacagttca acggcaatga ccgcctcatg tccgtctccc tcggcgacgg cgccacctcc
     1621 cggggctacg tggtggaaac cgcctccaac cagggccacg ccaccttgga gcggatcggc
     1681 gacaagacct accgaatcgc cggtgatgcc ctcgaagtgg actacaagac catgaacgaa
     1741 gcaggcacca agcccttcag cttcgacatc acctgcccct aaagcctccc cgctcatttc
     1801 tcccctccca cgacaggagc catccacccc atgaccacac acatctcccc cacgtttgcg
     1861 aagcgacttt acggcgcgct gggggcgtcg gtcctcctca tctccctcgg cgcctgcggt
     1921 cgggaggaag ccagggcggt gatgtacacg gccaccctgg acggcgagca attccagacc
     1981 gacgacccga ccgtcacctg cacggacaac ggcggcctga aggagatcac cgtgggccac
     2041 acccagcagg acgggggtga ggacgcggcc cccaccaact acatcaccgc gctcatcaac
     2101 cccgacggca cgttcgtgga ggacatcacc attagcgaca actccacctt caacttcacc
     2161 tatagcccct acagaaaaat cggatccgcc agcgccggcg aatccgatgg aacttacagc
     2221 atcaagggcg agggacagat gacccggctg gataaccagg accaggacac gcaggaccag
     2281 gacgccccag aaaagctggt gcccttcgag ctggagctca cctgcccgag cgaacactaa
     2341 aacgaacact cgggcagatc agctcgggca gctctgaagg gctccgagca acaactagat
     2401 gggactagtt gtggtcgtgg tggtcatggt cgtgaccatc gtggtcgtgg tggtcgtgat
     2461 cgtgaccgtc gtggtcgtgg tcatccgaac cgtaggactc gttgcccgca ccaatctcgc
     2521 ggccctcaac ctcggtctta aaggtctcgc cgtccttggt ctcaaagacg atggtcaggt
     2581 cgtcacctgg ctccacgtca tcacggaggt tcagcaccat caggtgctcg ccacctggct
     2641 tcagctcctt ggactcgccg gccttgatct cgaagccgcc ttccttcttc tgcatcttgc
     2701 cgtcaaccac ctcgtgaagc tcgaaggtag caccttcgtc cttcatgtcc tcgatggaga
     2761 agcccgtgat ggtgatgtcc ttatcggtgt tgttcaccag ggttccgaag caggcggtca
     2821 tgtcagccat ctcgccgtcc ttggccttat ctgcggatgg cttcgccttg cagaatccgt
     2881 tctcgaaggt caggccggtg ctggacttcg cggactccga tgcggaggac atcgcggagg
     2941 atgccgcgga cgttgccgac gatgcggcgg aggatgcaga gttctttgcc tcctcagcgt
     3001 cgtcggagct gcaggcggcg aggctaccac ccagggctag aacggataca acggcaaacg
     3061 cagacttctt catgcgcata taatctctcc ttatatgttg tcgcacagcg accctcggtg
     3121 agccgcgtgc agcacagtcg tggcacctgg gggtaacaca accaggctgt gacaccttca
     3181 gtctagccag gtatgcgtca gcgcggccgg cccgttgccc gcctttgggg ttagagcaac
     3241 acactgtgcg agcctcgggg tatctatgga cacagacccc tgcgggcata gagctagtcg
     3301 acggtcgtat ccttcgcggc ggccagacca cacagcaagc acagcaccga gacagccacc
     3361 atgatccaca gtcccgaagt ccactggccg gtggcgtcgt gcacagcacc caacaggatc
     3421 ggtccactgg ccgcgatgac atagcccatg ccctgcccca tgccggacag cgccgcggcg
     3481 gtttcgggtt tgcgagaacg cagggcgaac agggagaggc ttaaggcgaa ggcaaggcca
     3541 caggcgatac cttccacgaa gatcagcagc ccggcaatct gtggcgcgtg catgaggatg
     3601 atcagcggca cacccatcat gacggcgatg gcgaccacga tcgcccgttg cccacgcagg
     3661 cggctcagca gcacggagca actgaagcta ccgaccaggc tgccgatgtt catgaacgcc
     3721 tggtgcacgc cggcggcgcc cgcgctgatg cccatggatt gttcgatgga gctcatccac
     3781 gccacgatga cgtagaacat ggcggactgg aaccccatga atcccgtgat cgcccacgcc
     3841 aggggatgtt tccacgggtt gaaactgctg ctgggaaccg taccgacgcc ggcctcacgc
     3901 cccgcacggc gcccttgaat aatcagatgc gcgaatgctg ccagcgccat gacgcaccac
     3961 acggaggtgg ccagtcgcca tcccgcctct ccccacccgg aggcatggga gatgggcact
     4021 gcaagtcccg cggcgatggc agcgaatccc gcctgcacgg ccgcgtaggt acccgtcacg
     4081 aaaccgatgc ggtcgggaaa gtccctcttg accagcgcgg gaagtgtcac gttcaaagtg
     4141 gcgatagcca ggccgatgaa gccggtaccc acccacaaca acgcggcagc cgggagcgag
     4201 cgcacgatca agcccacggc cagcagtcct gtgccggcga tcagcgtgcg ctcgatcccc
     4261 cagcgcacga gcaaacgagg caccaggggc gaaacaatcg cgaagaccaa caacggcagc
     4321 gacgtcacga tggacacctg cgctcccgtg agaccaaggt cagcttggat gtcactcacc
     4381 acaggcgcca cggaagtcag cgccatgcga aggtttgcag caagtaagaa taatcccaag
     4441 agagctaaac cgaaggagcg ttgagtagac atatctacca tttgtactcc cctactttcg
     4501 gttactttca cccgggttcg atgtgttcta cccactgaga tcgccgcgct gtggtaagca
     4561 gtggtcacat gagcgattca caatcttctg cagcacacaa ctccgatcac actgaggatg
     4621 gacgctacga cgcgggcatc cgcgtccgcc gagaggtcat gggtgacgat tttgtggacg
     4681 cggccctgga gcgcgcggcc ggttccgatg gcgaggaatt gcaaaagcac gtcacggcga
     4741 ccgtgtgggg gtccgtgtgg acacgtgacg gtttgtcgaa gcgggatcgc tccctgctca
     4801 acatcgggat gctggtggcg ctgcgcgcca ctcaggagct gcgcggccac attaagggtg
     4861 ggctggctaa tgggctgacc cgagaggaga tcacggaggc gatcattcac tcctcgggat
     4921 actgtggcgc cccggcggcg ctgtccgcga tgaaggtcgc gcaagaggtg ctggaagcgg
     4981 agcttggccc gaaggacccg tcagcatagg gacttattct gttcatcccc tagggcggac
     5041 aaccagcggg acggggcttc cgcacgcgcc caggaatctc ctgccacaca gccagagtcc
     5101 acaaactgat ggccccgaac ggatgcgcta tgtggtgcgt catgcttaaa gtttgaccag
     5161 gtacgtccgc ccgaggatcc aaaatcccac tccccgccct cgtggggata caccgtctgc
     5221 gctccttgct cagaacctcc caggacaaga tagccccctc caccgagcag cagtgctgcc
     5281 ccggcgagcc cggcccacag agtcttcttt ttcttcatga gttttctcct agttctatcg
     5341 ttcggtccgt ctgcgcaatg acccagggat cgtggctcac tacgatgacc gccgcaccgt
     5401 ggtcagacag tgttcttaag tgcgtgagca cagaggctgc gttacttcta tccagggacg
     5461 ctgtgggctc atcagccagg ataacctcgg gctttcgcac tgaaagcctc gcaatagcaa
     5521 cacgctgttg ctcaccgcca ctgagctgat acacacgatg gttctcgtag ccctctagcc
     5581 ccacttcggc caacgcttgg gcaatgtctt cggtgagctg tcgtcctcgt gcacgactgc
     5641 ctaaggcgat attttggcgg acggtatcgt tatcaattag tgcataatcc tgaaaaagat
     5701 agcccacaga ttctcgacgc atacgcatcc gctgccgtgc ggaagcggat gctagaggga
     5761 tacctttgat gtcaatgtcg ccgttgtcag gccggtcgag caaccccaag caattgagta
     5821 gcgtggattt gccacacccc gaaggccctg tgacgctcac taattcgccg gaggacacat
     5881 cgaagctcag cttttcccac agccttcggc gtggcttctt ctctccgaca gccttcgaga
     5941 cattttttac cgaaaccatg acagtcattg cggaagtcat ctccttgtca ctacgagtga
     6001 gcgctgcgtc cttcgcccca ggacaaggcc gcttgcagga atgcttgtac taacgaacag
     6061 tgccagcgtt atccaccacg cgctactggg gacgtggaac agcgcaatca atgccgggtc
     6121 cgcggcacct tcggcggagc caccagcctc ccacgctcgc gcggttgccg cacgagtgaa
     6181 taagaaatac aacacgcagg cacacactat gagctcggca ccaagtgcag tcaggagagg
     6241 ccgaattggc caccgcgcat gcaggtacgc ggctcgaagc cgctggtgga acactttcat
     6301 gaagctcaac cacacagcca acgaggcgat gcagcacagc agtacagtgg caacaaatcc
     6361 accaacatag acccacagaa tccttcccat ggcctggtga tgctgggacc acgtctggct
     6421 catcggcacc gctgcgcgca cgaagcttcc gacttctcgg tcatcgtgca agcgttgaaa
     6481 atcatcataa gaactcagga gaacgtcatg ttgtgtgacg ctcgcgacga ggttgcgatc
     6541 ggacaggcca atcaggtcat taggcatcac aaccacaata ggatcatggg tttcagcacg
     6601 gttcatccac tcatccagcc ccacttccca ggtccgtgca cgggaatcac cggcacggta
     6661 aacagccgtg tgtaccctgg cctctcccac aatcggcgcc agcagctcat gactatccac
     6721 tgcggctggc agtgaatccg gcacaagcac caacggttgt ttatctctag gctgctcacc
     6781 gaagctacgg ctcacttcac ctgaaagtga gttttcggca gcagtggtgt tatacaccat
     6841 gatgtcccta tcaagatggc tgcctggggt catgccggaa tctcgaaatt gagcgtacag
     6901 cagtcgattc tgtgaggaga tgtccctcat atggctcgca agacggccac ttgcttcgtg
     6961 gagatcttcc aatttcctag caccgcttag agtaaggcca tacgccgaag gactattccg
     7021 ccaaccgtct acctcgtctt tttgctcatt ccactcagtt gtatagttca gtgctccaat
     7081 agacagcgac gcaacagcaa tgcacgcacc tatgcgtacg gcaaacatac tcaccaaaac
     7141 agactttgcg ggaagctttc cttttaatag ctgcaccaca ggggcgatct ttaagagaaa
     7201 aagacccaca aacaacgcga agatgcacgc cacaagcgcg atgccaataa aagctgcttg
     7261 gaaaagaaca agttggtatg cagaagcagt attggaatac caccacagga gtacgtcgac
     7321 cagaacaaca gcggcactag tggcggtaag taaggtacct ctcgcagcag agagctccgc
     7381 tttcgcacga ctcaacaatc cgattccatg cagccttcgc accgcatcgg tgtgggaatt
     7441 aagaacgatc ccaacaaaga ccaggacaaa gccaatgcct atcatcaggg ctgccatttt
     7501 ccctaatgcg gtgcctccca acaggtacat ccactgttgg ttctccggaa tgtgataggt
     7561 cactccgcgg gcgtccaagt aagataccag ctcttgtaga acgtcgtctc caccgatgac
     7621 ctcgaacatc tgccgataat caccgtgagg caagtcctcc agtggccgca catctaatgg
     7681 aggtgtccca ggcagtcctt gataaccctt gtgtatccag ctttcagcct tatcgcctgc
     7741 agcgtaagcc actcgcccct cgagcgttgg gatttcaacg aggattgtcg cgtcatgctc
     7801 ggtaacgatt ctgccgagat cacgtacaag agaatccgct gaggaatccc ctccctgatc
     7861 gtgcggcgcg aatgtttgaa tctgcacaac agacctcaca ccagccatct cggagtgctc
     7921 gatagttgag ctggcccaga atcccagaac tgcgccgagg gtgataacaa gcaccagggc
     7981 gcaccgaatt aattgtgtac cagacagcgc cgagacacgc cggcgctgct gtacatcatg
     8041 cgacttcgag aaatccaatg tcagtcccaa ttctcggaga tccgcctaca gtggcgagta
     8101 ctttttcgtg gcgccatgtc atctcctcaa agaacccaca ggattttaac caaacttagc
     8161 ctaaactaat acacagcatt taacaagaga tatacaacaa cgaagaactc ccgatcctga
     8221 tgtggatcgg gagttcttat cttgtacccc gtacgggatt tgaacccgtg ttaccggcgt
     8281 gagaggccgg cgtcctaggc cgctagacga acggggcgct ggcctaccag gactcgaacc
     8341 tagaatgacg gtaccagaaa ccgtagtgtt gccaattaca ccataggcca ttgttcgtgc
     8401 tgctcagcaa gcaaactccc cgaggtaatt tcttaccggt ggactctgtt cgctgtccgt
     8461 gcaacgagag taaatataac cagagaaatt acatgaatgc aaatcccctg gtcataagca
     8521 ctttttactc acatgaaacg ctgctcaaaa cgcgcgtttt atggcgggct actgctccgc
     8581 cacataggga gtctgagcct gagccgcctt caggcgtgcc agcgtggact ccttccccag
     8641 cagctccatg gactcaaaca gcggcgggga cacagcggca ccagagatgg ccacacgcag
     8701 cgctccatag gccttgcgag gcttgagctc catctcctca ataagcttcg actgcagagc
     8761 gtgctcaatg ttttccgtgc ggaactcctc ctcggacaat ccctccagct gagcaatcgc
     8821 cacctgcaaa acctctactg cgtcttcctt caggttcttc ttcgcggact tctcatccag
     8881 aaccagatcc tcatctgcgg tcaccaggaa tttcagcagg ccgtgggcgt cgcccaacat
     8941 cttgatgcgg gtctgcacta gctctgcggc gaaggcgaat ttgtcggctg ggtagtccgc
     9001 cgggaagtcc ttgtaggtct ccaggtactc gcgcaggcgc gtggtgaagt cctccagatc
     9061 cagcaggcgg atgtggtcag cgttaatggc ctccagcttc ttctgatcga agcgggccgg
     9121 gttgggcttc acgtcagcaa catcgaagtt gtcgatgagc tgctgcatgg agaagatatc
     9181 ctcgtcggca gagagggacc agcccagcag cgacaggtag ttcagcatgc cctcggggat
     9241 gatgccattg tcccggtggt tgaacaggtt ggactctgga tcgcgcttgg acagcttctt
     9301 gttgccttca cccatcacga agggaaggtg tccaaacgtc ggtacctgct gggccacgcc
     9361 gatgcgcacc agcgcctcgt agagggcgat ctggcgagga gtggacggaa gcaggtcctc
     9421 gccgcgcagc acgtgggtga tccccatgag cgcgtcatcc accgggttaa ccagcgtgta
     9481 cagcggctgg ccattggatc gtgcgaccac gaagtcaggg acggtcttgc cgtccacgtt
     9541 catctctcca cgcaccagat ccgtccacgt gtacgtgcgg ttatcaggca tgcgcaggcg
     9601 ccagacaggc tcgcggcctt ccgcttcaaa tgccgcgatc tgttcctcgg tcaagtcgcg
     9661 gtcgaagtta tcgtagccca gcttcgggtc gcgaccggcg gccttgtgac gctcttctac
     9721 ctctgccgcg gtggagtatg cggggtagac gtcgccggag gccttgagct tgtccagtac
     9781 ctcggcgtag atgtccatgc ggcgggactg gcggtacggc tcgtgcgggc cacccacctc
     9841 gatgccttcg tcccatccca ggttcaacca cttcaacgag tcgatgatgg cctggtagga
     9901 ctcttcggag tctcgggcgg catccgtatc ctcgatgcgg aaaatcagtt ttccgccggt
     9961 gtgccgggcg taggcccagt tgaacagggc cgtgcggacc atgccgacgt ggggggttcc
    10021 ggtgggagaa gggcaaaatc gtacgcgtac atcactcatg ggtactcact ttacctgccc
    10081 tacgccgccg ggcctgggtg cagcttgctc tgccgtgcta gaaagtccca ggccacgttg
    10141 gcggtgtcag gagcccacca ccacgtgtgg tcctgaccac gcagtttcca atgaacagcc
    10201 tcgtccgcac aaccggttcc gcgcatcact tccacgttgt tgaccagctc gctgacctga
    10261 ggggctccac tgcagccatt gcgctgttgg atggactgat acagcgtcgg cacaggca
//
