LOCUS       NZ_CP045704            10216 bp    DNA     linear   CON 03-SEP-2024
DEFINITION  Streptomyces rutgersensis strain NBH77 chromosome, complete genome.
ACCESSION   NZ_CP045704
VERSION     NZ_CP045704.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces rutgersensis
  ORGANISM  Streptomyces rutgersensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces diastaticus group.
REFERENCE   1  (bases 1 to 6848830)
  AUTHORS   Benaud,N., Edwards,R.J., Amos,T.G., D'Agostino,P.M.,
            Gutierrez-Chavez,C., Montgomery,K., Nicetic,I. and Ferrari,B.C.
  TITLE     Antarctic desert soil bacteria exhibit high novel natural product
            potential, evaluated through long-read genome sequencing and
            comparative genomics
  JOURNAL   Environ Microbiol 23 (7), 3646-3664 (2021)
   PUBMED   33140504
REFERENCE   2  (bases 1 to 6848830)
  AUTHORS   Benaud,N., Edwards,R.J. and Ferrari,B.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) School of Biotechnology and Biomolecular
            Sciences, University of New South Wales, Botany St, Sydney, NSW
            2052, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2018
            Assembly Method                   :: FALCON v. v 1.8.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014216335.1-RS_2024_09_03
            Annotation Date                   :: 09/03/2024 04:34:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,921
            CDSs (total)                      :: 5,831
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 102 of 189
            Pseudo Genes (incomplete)         :: 130 of 189
            Pseudo Genes (internal stop)      :: 14 of 189
            Pseudo Genes (multiple problems)  :: 50 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:08:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6082307
            Orig. end                         :: 6092523
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045704.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Belinda Ferrari, QAP Lab
            4002, Bioscience South E26, UNSW Sydney, Australia 2052 (Quarantine
            regulated isolate).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(764..781)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.94"
                     /tool="antismash"
     gene            complement(1650..3950)
                     /locus_tag="F0345_RS25455"
                     /old_locus_tag="F0345_25495"
     CDS             complement(1650..3950)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640763.1"
                     /locus_tag="F0345_RS25455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_25495"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_100453635.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.5e-34, bitscore:
                     117.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPADAPAAGTGPTAPPTPGGRRGRRAAPPSSGRRRAAPPRRRS
                     GRRLVRTPTVLQMEAVECGAACLAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAA
                     RGYGLTAKGMQMEPAALREVRAPAVLFWEFNHYVVHEGFLRRPGRRGAARLNDPDKGRR
                     VVSAEEFDASFTGVALLLEPGPSFRGGGRRPGLLGALPARLRGTGATLLTALLASLLLV
                     VVGAALPALSRTYIDLFLIGERGSVLGPLFAAMGALVALTASLTALQQANLLRGRIISS
                     TLSGARFLRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLARDLAAAAVDGVVVVLYA
                     LLLWTYDPQLTLVGVGIALLNIVAMRVVIHLRATRTRKLRADNARLTTTAYTGLQLIET
                     MKATGGEDGFFRRWAGQHATTLEEQQRLGVPTAVLSVVAPALATFNSALILWIGGLRAV
                     EGHLSVGLLVAFQALMVRFTAPLTRLNGVAGRIQDFAADVTRLRDVEAFPADGLYLREG
                     TAGGGADTRRLAGRVELDGLTFGYSPLDPPLLRDFSLTVGPGRQVALVGGSGSGKSTVS
                     RLLAGLYQPWEGTIRIDGRPLTEIPRPALAASVSFVDQDIFLFEGTVRENVTLWDPSLP
                     DEAVRTALRDAGLEEVVARRPDGVHARVEQDGRNFSGGQRQRLEIARALVRRPSVLVLD
                     EVTSALDAETEQRVMAALRRRGCACVIIAHRLSTVRDSDEIVVLDGGRVVERGRHAELV
                     ARGGAYAALVEEK"
     gene            complement(3947..4753)
                     /locus_tag="F0345_RS25460"
                     /old_locus_tag="F0345_25500"
     CDS             complement(3947..4753)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="F0345_RS25460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_25500"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_100453636.1"
                     /transl_table=11
                     /translation="MRFRQQALTKLQSPEQLDLPVRFARPQGLLVLAVTLVVMAAGGVW
                     AVTGSVATTLTAPGVLTHAQGSYIVQSTVPGQVTAVRTAEGRRVARGTPLLTVRTARGD
                     VTIRALAAGRITALAAVQGAVVTTGTDLAAVERQARADDPLLAVVYAPSDSGGSLSEGA
                     EVDLTVRSVPAQSYGVLRGTVKSVGRTPRTRQQISADLGSAQLGEEFSRGGPPIAVEVR
                     LRPSKSTRSGYTWSTSDGPPHPLTTLTLVDGAVHLDEQRPADWLLP"
     gene            complement(5001..5216)
                     /locus_tag="F0345_RS25465"
                     /old_locus_tag="F0345_25505"
     CDS             complement(5001..5216)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="F0345_RS25465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_25505"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_100453637.1"
                     /sec_met_domain="strepbact (E-value: 1.9e-35, bitscore:
                     119.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHTPQVETAEIADSELDNISGGLVSGLVGSVTAGVDSVAPVSET
                     LGAVTGTVEQTTGLNTAPVTGLVAGL"
     misc_feature    complement(5187..5202)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            complement(5833..6339)
                     /locus_tag="F0345_RS25470"
                     /old_locus_tag="F0345_25510"
     CDS             complement(5833..6339)
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640757.1"
                     /locus_tag="F0345_RS25470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_25510"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_124287890.1"
                     /transl_table=11
                     /translation="MRIKAVHHGELTALQEIERAAGVWFREAGMAEIADDEPLPAAELG
                     RYVRAGLAWSAVDGAGRPVAYLIADRVDGNLHVEQVSVHPACARRGVGRSLLDHAAGRA
                     RAEGAPALTLTTFTEVPWNAPYYARCGFRLLADRELTPGLREIRRSEAAHGLDRWPRSC
                     MRREV"
     gene            complement(6562..8379)
                     /locus_tag="F0345_RS25475"
                     /old_locus_tag="F0345_25515"
     CDS             complement(6562..8379)
                     /NRPS_PKS="Domain: AMP-binding (23-474). E-value: 6e-90.
                     Score: 293.7. Matches aSDomain:
                     nrpspksdomains_F0345_RS25475_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412454.1"
                     /locus_tag="F0345_RS25475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_25515"
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="WP_115069507.1"
                     /sec_met_domain="AMP-binding (E-value: 4.3e-84, bitscore:
                     280.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREVSVARLVAPQVSGGLADSVHQAAETHPDRPQLALEPVEAGGP
                     WRTVTAGRFRDEVLALARGLRAEGVGPGDRVVLMSRTRPEWTLLAYALWSLGAELVPVY
                     PTSSIEQVRWVLADAGVRAAVVEHSGQAMTVAAACAAPGTLLGLWQLDLGCADELVERG
                     AEVTEDEVRRLRAAVRPESTAVICYTSGTTGTAKGCVISHGNLAAECDTLIAGWGHLLA
                     TDGAQPSVLAFLPLAHCYGLMVAVTCVRGGYLLAHQPEMAPALLLPALASFRPTFLFAV
                     PYVFERIFDEACREAEAAGQGALFEKARAAAVAHAQAREERAAGTGHGPGPVARLRHSV
                     YERAVYSRVRAVFGGRVRAAVSGGSPLRRELGLFFAGAGVTVYDGYGLTETTAAVTAQP
                     PGRVRFGTVGRPLPGSTVRIADDGEVWVRGDTVFQGYLHGPPALAGGWLATGDTGRLDP
                     DGYLVITGRKKDIIITSGGKSVSPQLLEERLRRHPLIAQCLVVGDDRPYVAALLTVDPE
                     ALAHWRRLRGASGRAPAAPADDAELRAQLQRAVNQANTLVSRAESIRAFEVLPVDFTLQ
                     EGLMTPSFKLRRSAVAHAYADVIEALYAR"
     aSDomain        complement(6958..8310)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F0345_RS25475_AMP-binding.1"
                     /evalue="6.00E-90"
                     /label="F0345_RS25475_AMP-binding.1"
                     /locus_tag="F0345_RS25475"
                     /protein_end="474"
                     /protein_start="23"
                     /score="293.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="QAAETHPDRPQLALEPVEAGGPWRTVTAGRFRDEVLALARGLRAE
                     GVGPGDRVVLMSRTRPEWTLLAYALWSLGAELVPVYPTSSIEQVRWVLADAGVRAAVVE
                     HSGQAMTVAAACAAPGTLLGLWQLDLGCADELVERGAEVTEDEVRRLRAAVRPESTAVI
                     CYTSGTTGTAKGCVISHGNLAAECDTLIAGWGHLLATDGAQPSVLAFLPLAHCYGLMVA
                     VTCVRGGYLLAHQPEMAPALLLPALASFRPTFLFAVPYVFERIFDEACREAEAAGQGAL
                     FEKARAAAVAHAQAREERAAGTGHGPGPVARLRHSVYERAVYSRVRAVFGGRVRAAVSG
                     GSPLRRELGLFFAGAGVTVYDGYGLTETTAAVTAQPPGRVRFGTVGRPLPGSTVRIADD
                     GEVWVRGDTVFQGYLHGPPALAGGWLATGDTGRLDPDGYLVITGRKKDIIIT"
     CDS_motif       complement(7060..7113)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F0345_RS25475_0002"
                     /evalue="4.00E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="F0345_RS25475"
                     /protein_end="440"
                     /protein_start="422"
                     /score="12.9"
                     /tool="antismash"
                     /translation="GEVWVRGDTVFQGYLHGP"
     CDS_motif       complement(7771..7830)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F0345_RS25475_0001"
                     /evalue="3.60E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="F0345_RS25475"
                     /protein_end="203"
                     /protein_start="183"
                     /score="22.6"
                     /tool="antismash"
                     /translation="AVICYTSGTTGTAKGCVISH"
     gene            complement(8586..9506)
                     /locus_tag="F0345_RS25480"
                     /old_locus_tag="F0345_25520"
     CDS             complement(8586..9506)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612596.1"
                     /locus_tag="F0345_RS25480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_25520"
                     /product="hypothetical protein"
                     /protein_id="WP_124287889.1"
                     /transl_table=11
                     /translation="MRTALRTRPLATAAGGRGAAPGRRPLGRAVAGAVLAAATLFTTAP
                     RALADTSPSPSASQDEEAEAGPTEAGTAFRTAARLAQGQRGTASASTGDYLYWVFPADT
                     GQRPTIEATVSLPAEQARQGASTWRIDVYDGLRRRQPCTYGHQERRAAEDASEVRLTCT
                     LRTVRAFAEPWAHDPLPGAYYIRLTVSRLAADDLGRPFRAEVGVESQETGGSTAVGGKL
                     SGPLLPGISALPAASDEETADEDGEAQAPRPAALAAPEDGWSSGWWTDRWLWTAGGAVL
                     AALAGIFGYTLTRGSGRPHRVPPGV"
ORIGIN
        1 ccaggaagac caccgagacc accagcagca ccccgatcgc cgccagcgcc atcggcacgc
       61 tgtgcaccag cagcagcacc aggttgacgc cggccaccgt caccgactgc acggccgtcg
      121 gcccgattcc cgacagcacc cggcggatcg cgctgacacc catcgcggca ctggccagct
      181 ccccggtgga ccggtccgcg aagaacgccg tcggcagccg cagcagccgg tcccagacgg
      241 ccggctgcaa cgccgcctcg atccgtccct ccagacgcag caaggtcagc gactggagca
      301 gcgtgaacgc cgccgagacc accccggcca ccatcaccgc cacacacatc tgcgcgatca
      361 gcccggtccg cgcactcggc acgtacgcgc cgagcacctg cccggtcgcc accggcacca
      421 gggcgcccag tgccacggtg accagaccgc tgagggcgag gttgcgcagg tcgccccggg
      481 agccgcgcag gctgaacagc atcagccggc gcagcgtcag cggaccgccg ggcagcggcc
      541 ggtggagcat cgtggcgcgt tcctcgaagt cggcggcctc cttcgcgccg atccggatgc
      601 gggcaccggt ggccgggctg agcgcctcgt accggccgcg ccgccacagc agggcgacgg
      661 gggcgccgga cttggcgcgc cggcccacca gcggaccggc gtcggcgcgc caccacgcgc
      721 cggccagccg caccgggcgg gtccggagcc gtgcggcggc cgccatccgc tccaccggat
      781 cgagccgggc gccgcccgcc accgaacgcg ccgccgcctc cgggaggggg atgccgctgg
      841 cccgcgccac ctggcggaag accgcgtaca ccgcgtcgtc gcccgcctcg ccgggcgccc
      901 ggacggaccg gtcgcggcgg ccgatggagt cgagcagggc ctggtcggcg cgctcccgta
      961 ccgcctcgcc ggcgcggaca ccggccgccg cacggtcctc gtgggcccgc tccagccgct
     1021 ccacccaccc gtcgacggcg gtcagcagcc ggtgctgctg gtccaccagg gactgccaca
     1081 gcgccccgtc gaccagcagg tcggccgccg cctcggccgc gaagtcggcg ccgaggcgga
     1141 cgctgcccgg cggcacgtcc acccacagca cgtcctcgtc ggcgggcccc gccgccggcg
     1201 ccgggccgtc cgcctgcggc gcggcgaaca gcacggcgag tccccggccg aggccgagcg
     1261 ccaccgcgtg ctccagtggc cccggggccg gcgcccaccc ctcgtccgcc tcggggcggc
     1321 ccggcggcgc gtacagctcg cgcagcggca ggcggcgcag ggcgcaccgg gaggaggggc
     1381 ggcccaccag ggtgtggcgg ggaccctcga ccgcgcccag cagcagcgtg ccggcctcca
     1441 gacggcccag gaagtgccag tggccgtcct cggccgccgc caccgcgaag aggtcgaggc
     1501 cgcccccggt gaccagccag agcgcggagg ggccaccgag cggcagcacc gtcccgtcgg
     1561 ccgcctccac cggcgcgccg agcccgccga gtaccgccgt caccgggtcg gccgccgcgg
     1621 cacgggcggc ctgccgcaca cccgtcatct cacttctcct cgaccagggc cgcgtacgcc
     1681 ccgccccggg cgaccagttc ggcgtggcgg ccgcgttcca cgacgcggcc gccgtccagc
     1741 accacgatct cgtcgctgtc gcggacggtg gagagccggt gcgcgatgat cacgcaggcg
     1801 cagccgcgtc gccgcagggc cgccatcacc cgctgctcgg tctccgcgtc gagcgcgctg
     1861 gtcacctcgt ccagcaccag cacgctgggg cggcgcacca gggcccgggc gatctccagc
     1921 cgctgccgct ggccgccgga gaagttccgt ccgtcctgct cgacccgggc gtggacgccg
     1981 tcggggcggc gggcgaccac ctcctccagg ccggcgtcgc gcagcgccgt gcgcaccgcc
     2041 tcgtcgggga gcgaggggtc ccacagcgtg acgttctccc gcacggtgcc ctcgaagagg
     2101 aagatgtcct ggtcgacgaa ggagaccgag gcggccagcg ccgggcgcgg gatctcggtg
     2161 agcggccgcc cgtcgatgcg gatggtgccc tcccacggct ggtacaagcc cgccagcagc
     2221 cgcgagacgg tcgacttgcc gctgcccgaa ccgccgacca gcgccacctg gcgccccggg
     2281 ccgaccgtca gcgagaagtc cctcagcagc ggcgggtcca gcgggctgta gccgaaggtc
     2341 agcccgtcca gttccacccg gcccgccagc cgccgggtgt ccgccccgcc gccggccgtg
     2401 ccctcccgga ggtacagccc gtccgccggg aacgcctcca cgtcgcgcag ccgggtcacg
     2461 tccgccgcga agtcctggat gcggcccgcg acgccgttga gccgggtcag cggcgccgtg
     2521 aaccgcacca tgagcgcctg gaacgccacc agcagcccga ccgagagatg cccctcgacc
     2581 gcccgcagcc cgccgatcca caggatgagc gcgctgttga aggtggccag cgccggagcc
     2641 accaccgaca gcaccgccgt cggcaccccg agccgctgct gctcctccag cgtcgtcgcg
     2701 tgctgacccg cccaccggcg gaagaagccg tcctcgccac cggtcgcctt catcgtctcg
     2761 atgagctgga ggccggtgta cgcggtggtg gtcagccggg cgttgtccgc ccgcagcttc
     2821 cgggtccggg tggcccgcag gtggatcacc acccgcatcg ccacgatgtt cagcagcgcg
     2881 atccccaccc cgaccagggt gagctgcggg tcgtacgtcc acagcagcag cgcgtacagc
     2941 accaccacca ccccgtccac ggcggccgcc gccaggtcgc gggccagggt ctcggccacc
     3001 gtgtcgttcg actggagccg ctgcaccagg tcggcggggg agcgctgggc gaagaaggtc
     3061 accggcagtc gcagcagatg gcgcaggaac cgggcgccgc tcagcgtcga ggagatgatc
     3121 cgcccgcgca gcaggttggc ctgctggagc gcggtgagcg aggcggtcag cgccaccagc
     3181 gcgcccatcg ccgcgaagag cggcccgagc accgaaccgc gctccccgat caggaacagg
     3241 tcgatgtacg tccggctgag cgcgggcagc gcggcgccca cgacgaccag cagcaggctg
     3301 gccaggagcg ccgtcagcag ggtggcgccg gtgccgcgca gccgggcggg gagcgcgccg
     3361 agcagaccgg gccggcggcc gcccccgcgg aaggacgggc ccggctcgag gagcagggcg
     3421 acccccgtga aggaggcgtc gaactcctcc gcgctcacca cccggcgtcc cttgtcgggg
     3481 tcgttgaggc gcgccgcgcc gcgccgcccg gggcgccgca ggaacccttc gtggacgacg
     3541 tagtggttga actcccagaa cagcaccgcc ggggcccgca cttcccgcag cgcggccggt
     3601 tccatctgca tccccttcgc ggtcagcccg taaccgcggg ccgccttcag gacgttgctg
     3661 gcacgcgacc cgtcgcgcga gacgccgcag gcgatccgca gctcctccag cgggacgtgg
     3721 cgcccgtggt gggccaggac catcgcgagg caggcggcgc cgcactccac cgcctccatc
     3781 tggagcacgg tgggggtacg cacgaggcgc cgcccggacc tgcggcgcgg gggcgcggcg
     3841 cggcggcggc cggaggaggg cggggcggcg cggcggcccc ggcggccgcc cggggtgggc
     3901 ggggcggtgg ggccggtccc ggcggcgggg gcgtcggcgg gtgcggtcac gggaggagcc
     3961 aatcggccgg gcgctgttcg tcgaggtgga cggcgccgtc gacgagggtg agggtggtga
     4021 gcgggtgcgg cgggccgtcg gaggtcgacc aggtgtagcc gctgcgggtg ctcttcgagg
     4081 ggcgcaggcg gacctcgacg gcgatcggcg gcccgccccg ggagaactcc tcgccgagct
     4141 gggccgagcc gagatcggcg gagatctgct gccgtgtccg gggagtccgg ccgaccgact
     4201 tcaccgtgcc gcgcagcacg ccgtagctct gcgcgggcac cgaccgcacg gtgaggtcga
     4261 cctccgcgcc ctcgctgaga gaaccgccgc tgtcggaggg ggcgtagacc accgcgagga
     4321 gcgggtcgtc ggcgcgggcc tgtcgctcca cggcggcgag atcggtgccg gtggtgacga
     4381 cggctccctg gacggcggcc agcgcggtga tccgcccggc ggccagggca cggatcgtca
     4441 cgtcgccccg ggcggtgcgg acggtgagca gcggcgtgcc gcgcgcgaca cgccggcctt
     4501 cggcggtacg gacggcggtg acctggcccg ggacggtgct ctggacgatg tagctgccct
     4561 gggcgtgggt gaggacgccg ggagcggtca gagtggtggc gaccgaaccc gtcacggccc
     4621 agacgccgcc cgcggccatc acgacgaggg tgacggcgag gaccaggagc ccctgcgggc
     4681 gggcgaaccg caccggcagg tcgagttgtt cgggcgactg gagtttggtc agggcctgct
     4741 ggcgaaagcg cacgggagac ttcctcgact gccgggcacc ggccgctgcc ccggccgggg
     4801 ccggccggtg tcgccgtggt gcgccggacg cgtgcgcgcg ggtgcgccgg tgcgtccggg
     4861 agttcggggc ggacgtcgcg cggacgcccg ggggagggca agggggcggc cggtgcccgg
     4921 gacccgctcg ggtccccggc accggccggt gcggtccgcc gaagcgggcc gcacgcgtcg
     4981 tcgggcgacg cgcgggggtc tcagagaccg gcgacgaggc cggtgaccgg ggcggtgttc
     5041 agaccggtgg tctgctccac ggtgccggtg acggcgccga gggtctcgct gaccggggcg
     5101 acggagtcga cgccggccgt gaccgagccg acgaggccgc tgacgaggcc gccggagatg
     5161 ttgtccagct cgctgtcggc gatctcggcg gtctcgacct ggggggtgtg gctcatgggg
     5221 gaagttccct tcgcatcact gacggaaata gccactgcgg cgcactcgtg cggaagaagg
     5281 ggaaatcttt cgacggcccc cggacgaaag gcgctgaacc ggagatccgg aggcggtgcc
     5341 gcagtgcgat ggatcaaagc acgccggtgc gcggagcggc cagtcggcgg gagcggaatt
     5401 cggcggccgg tgccgttctt cttcccggcg cgtgcagaac caggcacagg aggacgtcag
     5461 caccttcaca ccgtctcgcc cgcgcgacgg acgaggtcac gtcggcgtgt acaccgcggg
     5521 cctcggtgtg aagccctggt gcggggcgtc gacctcggcc gtccgcacgg ggtgaaggta
     5581 tggccgacgg tgacggaggt gcggcacggc tgtgcagatt cgccgcaggt gaatccgtcc
     5641 tgggcttttc tggattcccg tacgggggcg ggtggaaaga ccgtgtcccg tgaatgtgga
     5701 gaaatggcgc ccggtgcggg aagaagaagg agcgggcgcg tgggaactgc ccccggccgg
     5761 cgaattcctg cgcactgcga aaaggccgcg gccaggcccg ccgggcgatt ctccgaggcg
     5821 gccgcaacgc gctcacacct cccggcgcat acagctgcgg ggccaccggt ccaggccgtg
     5881 cgccgcctcg ctccgccgga tctcccgcag gccgggcgtc agttcgcggt ccgccaggag
     5941 ccggaagccg cagcgtgcgt agtacggggc gttccacggc acctcggtga aggtggtcag
     6001 cgtcagggcg ggagccccct cggccctggc ccggccggcg gcgtggtcca gcagggaccg
     6061 gccgacgccc cggcgcgcgc aggccgggtg gaccgacacc tgctcgacgt ggaggttccc
     6121 gtccacccgg tcggcgatca ggtacgcgac aggccggccg gccccgtcga ccgccgacca
     6181 ggcgagcccc gcccgcacgt accggcccag ctcggcggcc ggcagcggct cgtcgtccgc
     6241 gatctccgcc atcccggcct cccggaacca cacgccggcg gcccgctcga tctcctggag
     6301 tgccgtcagt tccccgtggt gcaccgcttt gatccgcata cccgcatcgt cgggcgcgcg
     6361 tcggggcgcg ggcaacggga aaagggggcg gccccaccgg gccgaagggg ggaagcccgg
     6421 tggggccgag gcaacctgcc aggccagggg gaagggcctt caggtcggtc tgaggggcgt
     6481 gtgccccggc ccggcgcgcc catgcccctc ccggcgggct cttctccgca cggccgcgca
     6541 cggcccgcag cgccttcgcg gtcagcgcgc gtacagcgcc tcgatgacgt ccgcgtaggc
     6601 gtgcgccacg gcgctgcgac ggagcttgaa cgagggtgtc atcaacccct cctggagggt
     6661 gaagtcgacc ggcaggacct cgaaggcccg gatcgactcc gcccgcgaca ccagggtgtt
     6721 ggcctggttg accgcccgct ggagctgggc ccgcagctcc gcgtcgtccg ccggggccgc
     6781 cggcgcccgg ccggacgccc cacgcagccg ccgccagtgc gccagtgcct ccgggtcgac
     6841 ggtgagcagc gccgccacgt aggggcggtc gtcgccgacc accaggcact gcgcgatcag
     6901 cggatgacgg cgcaaccgct cctccagcag ttgcggggag acgctcttgc cgccgctggt
     6961 gatgatgatg tccttcttgc ggccggtgat gaccaggtag ccgtccgggt cgagccggcc
     7021 ggtgtccccg gtcgccagcc agccgcccgc gagcgccggc gggccgtgca ggtagccctg
     7081 gaagaccgtg tcgccgcgca cccacacctc gccgtcgtcg gcgatccgca ccgtcgaacc
     7141 cggcagcggg cggcccacgg tgccgaagcg gacccggccc ggcggctgcg cggtcaccgc
     7201 cgccgtggtc tcggtcagcc cgtagccgtc gtacaccgtc accccggcgc ccgcgaagaa
     7261 caggcccagt tcgcggcgga gcggcgagcc gcccgagacc gcggcgcgga cccgcccgcc
     7321 gaagaccgcc cgcaccctgg agtacacggc acgctcgtag accgagtgcc ggagtctggc
     7381 gacgggtccc gggccgtgcc cggtgcccgc cgcccgctcc tcccgcgcct gggcgtgcgc
     7441 gacggcggcc gcccgtgcct tctcgaagag cgcgccctgc ccggccgcct cggcctcccg
     7501 gcaggcctcg tcgaagatcc gctcgaagac gtaggggacg gcgaagagga aggtgggccg
     7561 gaaggaggcg agggcgggca gcagcagcgc cggggccatc tccggctggt gggcgaggag
     7621 atagccgccg cgtacgcagg tgacggccac catcaggccg tagcagtggg cgagcggcag
     7681 gaaggcgagg acggagggct gcgccccgtc ggtggccagc aggtggcccc accccgcgat
     7741 gagggtgtcg cactcggcgg ccaggttgcc gtggctgatc acgcagccct tggcggtgcc
     7801 ggtggtgccc gaggtgtagc agatgacggc cgtcgactcc gggcggaccg ccgcccgcag
     7861 ccgccgtacc tcgtcctccg tcacctcggc gccgcgctcg acgagttcgt ccgcgcaccc
     7921 caggtcgagc tgccagagcc cgagcagggt ccccggggcc gcgcaggcgg ccgccaccgt
     7981 catcgcctgc ccgctgtgct ccacgaccgc cgcccggacc cccgcgtcgg cgaggaccca
     8041 gcgcacctgc tcgatggagg aggtggggta gaccggtacc agctcggcgc ccagcgacca
     8101 cagcgcatac gccaggagcg tccactcggg gcgggtgcgc gacatcagta ccacccggtc
     8161 gcccggcccc acgccctcgg cccgcagccc ccgggccagt gccagcacct cgtcgcggaa
     8221 ccggcccgcc gtcaccgtgc gccaggggcc gcccgcctcg acgggctcca gggccagttg
     8281 agggcggtcc gggtgggtct cggccgcctg gtggaccgag tcggcgaggc cgccggagac
     8341 ctgcggggcg accaggcgcg ccacactcac ttcccgcacc gggtcgcctc cgttctcccc
     8401 ccgggccgcg tacacacccg tgctcggtcg ccgcgacccg gcccctcgcc cacctgacgg
     8461 gtggccggct cggcaaccta cagcgccccg cacgtgcggg ccaggggcga cgggggtgtt
     8521 caccaccgtg cgccggtggc ggctccgcgc gcctcccgcc gggggcgcgc ggagccggcg
     8581 cgccgtcaga cgcccggcgg tacccggtgg ggacggccgc tgccccgggt cagcgtgtag
     8641 ccgaagatgc ccgcgagggc ggccaggacg gcgcctcccg ccgtccacag ccaccggtcg
     8701 gtccaccagc cggacgacca gccgtcctcc ggcgcggcca gcgccgccgg gcgcggtgcc
     8761 tgcgcctccc cgtcctcgtc ggccgtctcc tcgtcggagg cggccggcag ggcggagatc
     8821 cccggcagca gcggtcccga gagcttcccg ccgaccgcgg tggagccgcc cgtctcctgc
     8881 gactccaccc cgacctcggc cctgaagggc cgccccaggt cgtcggcggc gagccgcgag
     8941 accgtcagcc ggatgtagta cgcgccgggc agcgggtcgt gcgcccacgg ctcggcgaac
     9001 gcccgcaccg tacgcagcgt gcaggtcagc cgcacctcgg aggcgtcctc ggcggcccgg
     9061 cgctcctggt gcccgtaggt gcagggctgg cggcggcgga gtccgtcgta gacgtcgatc
     9121 cgccaggtcg aggcgccctg ccgggcctgc tcggcgggga gcgacacggt cgcctcgatc
     9181 gtgggacgct gcccggtgtc ggcggggaag acccagtaga ggtagtcacc ggtcgaggcg
     9241 gaggccgtgc cgcgctgccc ctgcgcgagc cgcgcggcgg tgcggaacgc ggtgcccgcc
     9301 tcggtcgggc cggcctcggc ctcctcgtcc tgcgaggccg acggcgaggg ggaggtgtcg
     9361 gcgagcgccc gcggagccgt ggtgaacagc gtggcggcgg ccagcacggc ccccgcgacg
     9421 gcgcgcccga ggggacgccg gcccggtgcg gccccccgtc cgccggctgc ggtggccagg
     9481 ggccgggtac ggagagcggt acgcatcagt gggtcctcca gacggcgaag cgccagcgcg
     9541 acagccagcc gaagatcagc ccgccgagga acccggcgag taccagcaca cccagcagcc
     9601 accagccacg ccccagcccg aaggcggcgg tgtccgaggc gtcgtccggg ccgtcgacca
     9661 cgtcgaccgt cagctccagc ggcatgcccg gcgccgaggc gtcggcggcc ttggcggagg
     9721 cggcggcgaa ggagttgctg acctccaggc agaccggtgt cgcggccggc tcgccgccgt
     9781 cggcgtccgg gcgctcccgc tccgggtggc gcagccccga cgagatcacg tcggtccggc
     9841 cgctgcccgc ctcggagccg cgtaccagct cgcgcccgtg cgcggtcacc gcgcggagca
     9901 gtacgccgta gtcgtcggcg agcggccggt cggccgccac gctcaccgag gcgcgcagct
     9961 cctggccggg cagcacgtcc acccggtacc accggtgggt gcccagcggc tggcggtcgg
    10021 tgtacaggcc gggctcgatc ttcggggcct cggcgcaggc cgccgcaccc tccaccggca
    10081 ccggtgtgac caccgggtcg gccgcgcggt ccaccagctg cttcaccttg tcggagaggt
    10141 cgtccttgtg ctgcacggcg gtgtaggtgc cgccggtggc gtcggagatg caggagagct
    10201 gctggcggat cttggc
//
