LOCUS       NZ_CP045704            11278 bp    DNA     linear   CON 03-SEP-2024
DEFINITION  Streptomyces rutgersensis strain NBH77 chromosome, complete genome.
ACCESSION   NZ_CP045704
VERSION     NZ_CP045704.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces rutgersensis
  ORGANISM  Streptomyces rutgersensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces diastaticus group.
REFERENCE   1  (bases 1 to 6848830)
  AUTHORS   Benaud,N., Edwards,R.J., Amos,T.G., D'Agostino,P.M.,
            Gutierrez-Chavez,C., Montgomery,K., Nicetic,I. and Ferrari,B.C.
  TITLE     Antarctic desert soil bacteria exhibit high novel natural product
            potential, evaluated through long-read genome sequencing and
            comparative genomics
  JOURNAL   Environ Microbiol 23 (7), 3646-3664 (2021)
   PUBMED   33140504
REFERENCE   2  (bases 1 to 6848830)
  AUTHORS   Benaud,N., Edwards,R.J. and Ferrari,B.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) School of Biotechnology and Biomolecular
            Sciences, University of New South Wales, Botany St, Sydney, NSW
            2052, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2018
            Assembly Method                   :: FALCON v. v 1.8.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014216335.1-RS_2024_09_03
            Annotation Date                   :: 09/03/2024 04:34:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,921
            CDSs (total)                      :: 5,831
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 102 of 189
            Pseudo Genes (incomplete)         :: 130 of 189
            Pseudo Genes (internal stop)      :: 14 of 189
            Pseudo Genes (multiple problems)  :: 50 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:08:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5710820
            Orig. end                         :: 5722098
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045704.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Belinda Ferrari, QAP Lab
            4002, Bioscience South E26, UNSW Sydney, Australia 2052 (Quarantine
            regulated isolate).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11278
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11278
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11278
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6278](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6278)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            987..2462
                     /gene="hemG"
                     /locus_tag="F0345_RS23890"
                     /old_locus_tag="F0345_23915"
     CDS             987..2462
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404755.1"
                     /locus_tag="F0345_RS23890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23915"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_100457211.1"
                     /sec_met_domain="DAO (E-value: 2.7e-08, bitscore: 32.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAETDGNPAGADGTSPRVVVVGGGVAGLTAALRLLDAGAAVTLL
                     EASERLGGKLRTGDIGGVRVDLGAESMLARRPEAVALAREAGLAGALQPPATASAALWT
                     RDALRPMPKGHVMGVPADPASLAGVLSEEGVARIAEDRTLPRTEVGEDIAVGEYVAARV
                     GREVVDRLVEPLLGGVYAGNAYRISLRAAVPQLFAAAREHTSLTEGVRAIQARTAAADP
                     RTDPVFMGIDGGVGRLPQAVADTVRARGGRIRTGTPVTGLARIPGGWRLTTGTPAAPEA
                     LDADAVVLAVPAPQAARLLAAELPAAAADLAEIEYASMALMTFAFRAGEAALPQGSGFL
                     VPPVDGRTVKAATFAGRKWRWIAEQEPGLQIVRTSVGRHREEEMLGRDDAELLDLARRD
                     LRTATGVAAEPVDAHVTRWDQGLPQYPVGHLARVARVRDQVAGARGLAVCGAAYDGVGI
                     PACVASATEAARQVLAPRPEGDPASGSSGHAGE"
     gene            2467..3183
                     /gene="hemQ"
                     /locus_tag="F0345_RS23895"
                     /old_locus_tag="F0345_23920"
     CDS             2467..3183
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="F0345_RS23895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23920"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_100457210.1"
                     /transl_table=11
                     /translation="MSAASEKNPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAD
                     EVQDLFDKLAAKDVTVRGTYDVSGLRADADVMIWWHAETADALQEAYNLFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYEWYLLPDEERRAMLADH
                     GRMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARRHVREEVPF
                     YTGRRKSVADLVAQLA"
     gene            complement(3197..4864)
                     /locus_tag="F0345_RS23900"
                     /old_locus_tag="F0345_23925"
     CDS             complement(3197..4864)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-286). E-value:
                     5.5e-16. Score: 50.8. Matches aSDomain:
                     nrpspksdomains_F0345_RS23900_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404759.1"
                     /locus_tag="F0345_RS23900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23925"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_185393526.1"
                     /sec_met_domain="PF00561 (E-value: 2.8e-19, bitscore: 68.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPLIFTTATALLLAPLAALPQAPAAAAPAPPGGAEARGVALAA
                     ERAARTPVVFGRCPDEEGLPRSLECGTVRVPLDYAAPDGKQIDLTVSRVRAAGPARERQ
                     GALVFNPGGPGASGMLFPLVGIFPEWKRLGSVYDLVGYAPRGVGRSAPLSCRDPREKPD
                     GTWQAPEHPTEEQKAERIAVAKAYARGCAERSGERLRHVTSLNNARDLEVLRAGLGERK
                     LTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPTPSRVWYRNNLAQSAAFEERWADF
                     RRWIASHDAVYGMGSDGAQVLAGYRRAREKLAAEPAGGRVGPAQLQAVMLRAGYYEDYW
                     PSHAGALAAYLKGDPQPLVNLATPLEEGAAEAENARAVYTAVECNDAPWPTRWETWDRD
                     HTRLARRAPFETWENAWLNLPCAYWKEPRQSPLDVRVRPGELPGTLILAGERDAATPYA
                     GALELRRRLPGASLVTERGAGTHGLAGGPNACVNQHVEAYLIDGKLPGRSTDCAARPAP
                     EPESQDASSPAGDEADGNGGRALRPALPRAAGTRPAAR"
     aSDomain        complement(4007..4549)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F0345_RS23900_Abhydrolase_1.1"
                     /evalue="5.50E-16"
                     /label="F0345_RS23900_Abhydrolase_1.1"
                     /locus_tag="F0345_RS23900"
                     /protein_end="286"
                     /protein_start="105"
                     /score="50.8"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASGMLFPLVGIFPEWKRLGSVYDLVGYAPRGVGRS
                     APLSCRDPREKPDGTWQAPEHPTEEQKAERIAVAKAYARGCAERSGERLRHVTSLNNAR
                     DLEVLRAGLGERKLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPTPSRVWYRNNL
                     AQSAAFEERWADFRRWIA"
     gene            complement(5001..6278)
                     /locus_tag="F0345_RS23905"
                     /old_locus_tag="F0345_23930"
     CDS             complement(5001..6278)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641256.1"
                     /locus_tag="F0345_RS23905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23930"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_238156488.1"
                     /sec_met_domain="DUF692 (E-value: 6.6e-89, bitscore: 296.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGIEWVEVVAENICPGHLPDSLRRLRERGVAVVPHGVGLGLGGA
                     ERPDPVRLAALAERAQALGAPLVTEHIAFVRAGGERTASPRLEAGHLLPVPRTREALAV
                     LCENVRIAQDQLPVPLAVENIAALFSWPDEELTEGQFLHELADRTGVRLLIDVANLHTN
                     HVNLGEDPSAALDALPLEALAYVHVAGGFERDGVWHDSHAHPVPEPVLAVLAELASRVR
                     PPGVLLERDERFPSAAELAAEVARIRRTVASAGRTPVLVGARPAAGPGAGPTGPGAAKV
                     PAKAAGETVRTRLAIGQTALLSALVAGTPAPEGFDRGRLRVQSRMLAAKRGDVVAFLAP
                     ELPRVLGDTYRPRFLRYAAGRPLTGGHRSDALAFALHTLRHDPPAGRRARRELRDWWIE
                     RSGRASLVGRPWARWARVLRFALLGR"
     gene            complement(6539..7252)
                     /locus_tag="F0345_RS23910"
                     /old_locus_tag="F0345_23935"
     CDS             complement(6539..7252)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894649.1"
                     /locus_tag="F0345_RS23910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23935"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_100457207.1"
                     /transl_table=11
                     /translation="MTGEPLSDSPFRDERTARDEAPQFVLPLVVRLERAEPPARTDALE
                     TAARAVLTLLCDPRSEGDGEWARAVRDWQDARIRKVVRRARGSEWRKAAALPGITVEDG
                     GAEVRVHPPVPLDGWPRELAKLQVSGTELEDPEEPAEPAPGVPVLWLNPDVPMSAGKAM
                     AQAGHGAQLAWWRLSAAERDAWREQGFALAVRTAGAARWKELVESGLPLVRDAGFTEIA
                     PGSCTVVAEHPALVG"
     gene            complement(7417..8286)
                     /locus_tag="F0345_RS23915"
                     /old_locus_tag="F0345_23940"
     CDS             complement(7417..8286)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951592.1"
                     /locus_tag="F0345_RS23915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23940"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_185393799.1"
                     /transl_table=11
                     /translation="MVRKARTRTGTALGALMALGAVAGCGQSAPAPHDTAPGPHASHSA
                     SPTVPAAPPTLAPGPGGLAPVFANGPRNGGRTVALTFDADMTADQGERAAGGERFDHPE
                     LIASLRRLKVPATVFMTGRWAEEYPDQAKAIGADPLFEVANHSYSHHAFTGDCYGLPRV
                     ARSEMRADVERAYTAFRAAGVKKAMPYFRFPGGCYDDAALRAVSAAGVTAVQWDVVSGD
                     AFATDADAVAQQVLDGVRPGSVVVMHCTRSAAPATERALRRIVPALREQGYRFVKVSEL
                     IGAASRKG"
     misc_feature    complement(8347..8356)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(8735..10099)
                     /locus_tag="F0345_RS23920"
                     /old_locus_tag="F0345_23945"
     CDS             complement(8735..10099)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951595.1"
                     /locus_tag="F0345_RS23920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_23945"
                     /product="alkaline phosphatase PhoX"
                     /protein_id="WP_124287964.1"
                     /transl_table=11
                     /translation="MSATRRQVLARTGAVAGIAFTGSLTELFAGTAAAKGMGRAGYGPL
                     VPDPKGLLDLPKGFRYRVLSREGDRMLSGEGEVPSNHDGMAAFGGRRGQVHLVRNHENR
                     TDAEIRVPAVRGLTYDPEGLGGCTALTLDSANRVLGERVAIAGTSTNCAGGPTPWGTWL
                     TCEETEDKAGTNGYTKDHGFIFEVDPTNPHRTGAVPLEAMGRFQHEAVAIDPRNGVVYE
                     TEDAFERPFGLFYRFLPVKPRGGLGSLRAGGSLQAMRVPGVPDLSAIQETGAAFDRVEW
                     VDVPDPLAAETPIRFQDFGPKGTTHAQKLEGCYWGGSSVYFVSSFARSNEGSAADHYGQ
                     IWRYDPARRRLTLVVVFGPDTDVQLPGEEPDNICLAPSGGLMVAEDGEGAQHVFGVTTR
                     GEVYPMARGRQNIGTPEEPAWGEFAGICFSPDYDTMFVNCYTPGTTFAVTGPWHR"
     misc_feature    10528..10540
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.92"
                     /tool="antismash"
ORIGIN
        1 ccgccacggt cagccagcgc agcgtcgccg tcaacgccga ccagccgccg gagagcgcgt
       61 cggtgaaggt cgggccgtcg tccccggcgg gctccgcctt cgccgcgcgc tcggagagct
      121 ggagggtgac cgtcgccatc gaggtgcggt ccttcaggga cgactgctgg gcgagcagcg
      181 actccagctc cgcctggcgg ctgctcagct ccccctccag cgccacgacg tcgctcagct
      241 tctccgcccg gtccatcagc tcgcggaccc gcgcgacact ggcccgctgc gacttcaggc
      301 ggctctcgac gtccaccacc tcctcggtga cgtcctccgc gccggtccgc cgcgacacca
      361 cctccccgcc ctcgccgagc gagtccagca ccgcgtcgta ccgctcctgc ggcacccgca
      421 ccaccaccgt cgaccgctcg tgccctcgcg cgtcgcggcg ggtcgactct tcaccgacgt
      481 agccgcccgc gtcctcgacc accgagcggg ccgacgccag cgccgccggt acgtccttca
      541 cccgcagctc cagctcggcg gtccggacca ggtggacgcc cttcagctcc ggagtcctcc
      601 gcccggccgc ctcgccgctc cggtccgcgc cgtccccctg ccgttcggcc tggccgccgc
      661 ccgccccgtc cgccgacgac ggggccgccg cccgctccgg caccgacgag gccgactccg
      721 aggagccgga cgaacacccc gccagcccca gtaccgccac cgccagcagc gccgtcgccc
      781 ggcgtttcgc gatcccgccg ccgttgcctg tccgcgtccg gctcatccgc cgtccccctc
      841 gtcctcgccg ccccacccgt cggggcgcct gtgccgctac gacgccgccg cgcaccggcg
      901 ggtttcggct ccggggtcgc gaacgggtaa cggaaccacg ccgacccggc ggcggcccgg
      961 cccgcccggt ttgagacagt gactccatgc gcgcggaaac agacggaaac ccggcgggag
     1021 cggacggcac gagcccccgc gtggtggtcg tcggaggcgg cgtcgcgggc ctcacggccg
     1081 ccctgcgcct cctcgacgcc ggagccgccg tcaccctcct ggaagcctcc gaacggctcg
     1141 gaggcaagct ccgcaccggc gacatcggcg gcgtccgcgt cgacctgggc gccgagtcga
     1201 tgctggcccg gcgccccgag gccgtggccc tggcccgcga ggccggactc gccggcgccc
     1261 tccagccccc cgccaccgcc agtgccgccc tgtggacccg cgacgccctg cgccccatgc
     1321 ccaagggcca cgtcatgggc gtccccgccg acccggccag cctcgccgga gtcctctccg
     1381 aggagggcgt cgcccggatc gccgaggacc gcaccctgcc ccgcaccgag gtcggcgagg
     1441 acatcgccgt cggcgagtac gtcgccgccc gcgtcggccg cgaggtcgtc gaccgcctcg
     1501 tcgagcccct cctcggcggc gtctacgcgg gcaacgccta ccgcatctcc ctgcgcgccg
     1561 ccgtccccca gctcttcgcc gccgcccgcg agcacacctc gctgaccgag ggcgtccgcg
     1621 cgatccaggc ccgcaccgcc gccgcggacc cgcgcaccga ccccgtcttc atgggcatcg
     1681 acggcggcgt cggccgcctc ccgcaggccg tcgccgacac cgtccgcgcc cgcggcggcc
     1741 gcatccgcac cggtacgccc gtcaccggcc tggcccgcat tcccggcggc tggcgcctga
     1801 ccaccggcac gcccgccgcc ccggaggcgc tcgacgccga cgccgtcgtc ctcgccgtgc
     1861 ccgcgccgca ggccgcccgg ctgctcgccg ccgaactgcc ggccgccgcg gccgacctcg
     1921 ccgagatcga gtacgcctcc atggccctga tgaccttcgc cttccgcgcc ggggaggccg
     1981 ccctgccgca gggcagcggc ttcctcgtcc cgcccgtcga cggacggacc gtcaaggccg
     2041 ccaccttcgc gggccgcaag tggcgctgga tcgccgagca ggaaccgggc ctccagatcg
     2101 tccgcacctc cgtcggccgc caccgcgagg aggagatgct cggccgcgac gacgccgaac
     2161 tcctcgacct ggcccgccgc gacctgcgca ccgccaccgg cgtcgccgcc gaaccggtcg
     2221 acgcccacgt cacccgctgg gaccagggcc tgccgcagta ccccgtcggc cacctcgccc
     2281 gcgtcgcccg tgtccgcgac caggtcgccg gggcccgggg actcgccgtg tgcggcgccg
     2341 cctacgacgg cgtcggcatc ccggcctgcg tcgcctccgc gaccgaggcg gcccgccagg
     2401 tcctcgcgcc ccgcccggaa ggcgacccgg cgtcgggctc gtccgggcac gcgggagaat
     2461 gaccccatga gtgctgcttc cgagaagaac cccaacgcgg gcaagaaggc caaggacctc
     2521 aacgaggtca tccgctacac gctgtggtcc gtcttcaagc tccgcgacgt gctccccgag
     2581 gaccggtccg gctacgccga cgaggtccag gacctgttcg acaagctcgc cgccaaggac
     2641 gtcaccgtcc gcggcaccta cgacgtctcc ggtctgcgcg ccgacgccga cgtgatgatc
     2701 tggtggcacg ccgagaccgc cgacgccctc caggaggcgt acaacctctt ccgccgcacc
     2761 cgcctcgggc gcgcgctgga gccggtctgg tcgaacatgg cgctgcaccg ccccgccgag
     2821 ttcaacaagt cgcacatccc ggcgttcctc gccgacgaga cgccgcgcga ctacatcagc
     2881 gtctacccgt tcgtccgctc ctacgagtgg tacctcctcc ccgacgagga gcgccgggcg
     2941 atgctcgccg accacggcag gatggcccgc ggctacccgg acgtgcgcgc caacacggtc
     3001 gcctcgttct ccctcggcga ctacgagtgg atcctcgcct tcgaggccga cgagctgtac
     3061 cgcatcgtcg acctcatgcg ccacctgcgc gcctccgagg cacgccgcca cgtccgcgag
     3121 gaggtgccct tctacaccgg gcgccgcaag tccgtcgccg acctggtcgc ccagctcgcc
     3181 tgagaccgga cccgtctcac cgggccgccg gacgcgtccc ggcggcccgg gggagcgccg
     3241 gccgcagcgc gcggccgccg ttcccgtccg cctcgtcccc ggccggggac gaggcgtcct
     3301 gcgactccgg ctccggagcc ggccgggccg cgcagtcggt gctccgcccg gggagcttcc
     3361 cgtcgatcag gtaagcctcg acgtgctggt tgacgcaggc gttgggcccg cccgccagcc
     3421 cgtgcgtccc cgcgccccgc tcggtcacca gcgaggcgcc cggcagccgc cgccgcagct
     3481 ccagcgcccc ggcgtacggg gtcgcggcgt cccgttcccc ggcgaggatc agcgttccgg
     3541 gcagctcacc cggccgcacc cgtacgtcca gcgggctctg ccggggctcc ttccagtacg
     3601 cgcacggcaa attcagccag gcgttctccc aggtctcgaa cggcgcccgg cgggccagcc
     3661 gggtgtggtc ccggtcccag gtctcccaac gcgtcggcca cggcgcgtcg ttgcactcca
     3721 ccgccgtgta gacggcccgc gcgttctccg cctccgccgc gccctcctcc agcggcgtcg
     3781 ccagattgac cagcggctgc ggatcgccct tcaggtaggc cgccagcgcc ccggcgtgcg
     3841 agggccagta gtcctcgtag tagccggccc gcaacatcac cgcctggagc tgcgccggcc
     3901 cgacccggcc cccggccggc tccgccgcca gcttctcccg tgcccgccgg taccccgcca
     3961 gcacctgtgc cccgtcgctg cccatcccgt acaccgcgtc gtgcgaggcg atccaccgcc
     4021 ggaagtccgc ccaccgctcc tcgaacgccg ccgactgggc cagattgttc cggtaccaga
     4081 cgcgcgaggg ggtcgggtcg accgccgagt cgaagaccat ccgccgcacg tgcgagggga
     4141 agagcgtcgc gtacagcgcc ccgaagtacg tgccgtacga cgcgcccatg aacgtcagct
     4201 tccgctcacc gagaccggct cgcagcacct ccaggtcccg ggcgttgttc agcgaggtga
     4261 cgtgccgcag ccgctccccg ctccgctccg cgcacccccg cgcgtacgcc ttggccaccg
     4321 cgatccgctc cgccttctgc tcctcggtcg gatgctcggg cgcctgccac gtcccgtccg
     4381 gcttctcacg cgggtcccgg caggagagcg gcgccgaccg gcccaccccg cgcggcgcgt
     4441 acccgaccag gtcgtagacc gaccccagcc gcttccactc ggggaagatc cccaccagcg
     4501 ggaagagcat ccccgaggcg cccggcccgc ccgggttgaa gaccagcgcg ccctgccgct
     4561 cccgcgccgg accggccgcc ctcacccggc tcaccgtcag gtcgatctgc ttcccgtcgg
     4621 gcgccgcgta gtccagcggg acccgcaccg tgccgcactc cagcgaccgg ggcagcccct
     4681 cctcgtcggg acaccgcccg aacacgaccg gggtcctcgc cgcccgctcg gccgccagtg
     4741 ccaccccgcg cgcctccgcg ccgccgggcg gggccggggc agcggcggcg ggcgcctgcg
     4801 ggagggccgc gagcggggcg aggagcagcg ccgtggcggt ggtgaagatg agcggtgcgc
     4861 gcatcgcgtc cctttcgtcc ggccggtgaa cggcagaagg gatgattcgc acggcggccg
     4921 gcgagggcaa gcaccggcgg acgtgttggc ccgacccgct cggatcggcc caacaccgcc
     4981 gtccggggac ggcgccgtgc tcagcgaccc agcagcgcga agcggagcac ccgcgcccac
     5041 cgggcccacg gcctgcccac cagcgacgcc cggcccgacc gctcgatcca ccagtcgcgc
     5101 agctcacgcc gcgcccggcg cccggccgga ggatcgtgcc gcagcgtgtg gagcgcgaag
     5161 gcgagcgcgt ccgaacggtg accgccggtc agcggacggc ccgccgcgta ccgcaggaac
     5221 cgcggccggt acgtgtcgcc gaggacgcgc ggcagctccg gggcgagaaa ggccaccacg
     5281 tccccgcgtt tggccgccag catcctgctc tgcacccgca gccggccccg gtcgaacccc
     5341 tccggcgccg gagtccccgc caccagcgcc gacagcagcg ccgtctgccc gatcgccagc
     5401 cgtgtccgga cggtctcgcc cgccgccttg gcgggcacct tcgccgcgcc ggggccggtc
     5461 ggacccgctc ccggaccggc cgccggccgc gcccccacca gaaccggggt ccgcccggcc
     5521 gacgccaccg tccggcggat cctcgcgacc tccgccgcca gttcggccgc cgaggggaac
     5581 cgctcgtcgc gctccagcag caccccgggc ggccgcaccc gcgacgccag ctcggccagg
     5641 accgcgagca ccggctccgg caccgggtgg gcgtggctgt cgtgccacac cccgtcccgc
     5701 tcgaagccgc ccgccacatg gacgtacgcc agcgcctcca gcggcagcgc gtcgagggcg
     5761 gccgacgggt cctcacccag gttgacgtgg ttggtgtgca ggttggcgac gtcgatcagc
     5821 agccgtacgc cggtccggtc ggccagctcg tggaggaact ggccctccgt cagctcctcg
     5881 tccggccagg agaagagggc cgcgatgttc tccacggcga gcggcacggg cagctggtcc
     5941 tgggcgatcc gcacgttctc gcagagcacc gcgagcgcct cccgcgtccg gggcaccggc
     6001 agcaggtgcc cggcctccag ccggggcgag gcggtccgct caccgcccgc cctgacgaac
     6061 gcgatgtgct cggtgaccag cggcgccccc agcgcctggg cccgctcggc cagcgccgcg
     6121 agccggaccg gatcgggccg ctccgcgccg cccagcccga gacccacccc gtgcggcacc
     6181 accgccacac cgcgctctcg cagccgccgc agcgaatcag ggaggtgacc ggggcagatg
     6241 ttctccgcca ccacctccac ccactcgatc cccgccatcg cctcgaccgc ctcagcgatc
     6301 gcgggccgcc acccgatccc cgtccccagc cgctcagccc cgacctgtgc cacctgactc
     6361 ccccttcagg acgccccctt gccctgcggc caccctgccc gtcgccactc ggcccggaac
     6421 cgtgtcccgc ccacttcaga gcaggatttg agcttcaccg cccgcccacg ccgcgccacg
     6481 ccccgccccc gccggcccgc cgaggaggcg ccgccgggcc acgccccgcg tgccggcctc
     6541 accccaccag cgcggggtgc tccgccacca ccgtgcacga cccgggcgcg atctcggtga
     6601 acccggcgtc ccgcaccagg ggcagtccgc tctccaccag ctccttccag cgcgcggccc
     6661 ccgcggtccg caccgccagc gcgaacccct gctcgcgcca ggcgtcccgc tccgccgccg
     6721 acagccgcca ccaggccagt tgcgcgccgt gccccgcctg ggccatcgcc ttccccgccg
     6781 acatgggcac gtccgggttg agccagagca ccggtacgcc gggcgcgggc tcggccggct
     6841 cctccggatc ctccagctcg gtgccggaga cctggagctt ggccagttcc ctgggccagc
     6901 cgtcgagcgg caccggcggg tgcacgcgga cctcggcgcc gccgtcctcg accgtgatcc
     6961 ccggcagcgc cgcggccttg cgccactccg acccccgggc ccgccgcacc accttgcgga
     7021 tacgcgcgtc ctgccagtcc cgtaccgccc gcgcccactc tccgtccccc tccgaccggg
     7081 ggtcgcagag cagtgtcaga acggcccgcg cggccgtctc cagcgcgtcg gtgcgggcgg
     7141 gcggctcggc ccgctccagc cgcacgacga gcggcagcac gaactgcggc gcctcgtcgc
     7201 gggcggtgcg ttcgtcacgg aagggactgt cggagagggg ttcgccggtc accgcaccag
     7261 tctgccaaac ccgggggcgc cgcccgcacc ggccggaagg acgccgcccg acaggcgcgc
     7321 ggggttcccg gaccgcacgc ggacgacgac ccgtccgccc ctcccgctcg tccgtgagac
     7381 cccctcggag accgcgactg aggcggacgg gcagcctcac cccttccgcg aagccgcccc
     7441 gatcagctcg gacaccttca cgaagcggta cccctgctcg cgcagcgcgg gcacgatccg
     7501 gcgcagcgcc cgctcggtgg ccggcgcggc gctgcgggtg cagtgcatga cgacgaccga
     7561 gcccggccgc accccgtcca gcacctgctg cgccaccgcg tcggcgtcgg tggcgaaggc
     7621 gtccccgctc accacgtccc actgcacggc cgtcaccccg gccgccgaga cggcccggag
     7681 cgccgcgtcg tcgtagcatc cgccggggaa ccggaagtac ggcatcgcct tcttcacccc
     7741 ggccgcgcgg aaggcggtgt aggcccgctc cacgtcggcc cgcatctcgc tccgcgcgac
     7801 gcgcggcagc ccgtagcagt cgccggtgaa ggcgtggtgg ctgtacgagt ggttggcgac
     7861 ctcgaagagc gggtcggcgc cgatggcctt ggcctggtcc gggtactcct cggcccaccg
     7921 cccggtcatg aagacggtgg cgggcacctt caggcgccgg agcgaggcga tcagctcggg
     7981 gtggtcgaag cgctcaccgc ccgccgcccg ctcaccctgg tcggcggtca tgtcggcgtc
     8041 gaaggtgagg gcgacggtcc tgccgccgtt ccgtgggccg ttggcgaaga ccggggcgag
     8101 tccgccgggt cccggcgcca gcgtgggcgg cgccgccggg acggtggggg aggcggagtg
     8161 cgaggcgtgg ggccccggcg ccgtgtcgtg gggggccggg gcggactggc cgcagccggc
     8221 caccgcgccc agcgccatca gggcgcccag cgccgtgccg gtccgggtcc ttgccttacg
     8281 caccatgctg atcacggcgc gaccctagga tgagaaaagc cgcatatcgg gaaaaacgcc
     8341 gcagggggcc gccttctcac ccgtctcgcc ccacccgccg cccgccatcc ggtgccccgg
     8401 acgcggtccc ccgcgggccg ccggggacgt ccgcgcccag aagcggccgg ctgcggcgcg
     8461 ccgtgcgcgg cctcggtcgg tcacgcgcac ccgcggcccg cgcgacgccg tggacccacg
     8521 gcggcccgcc ggaccgccgg agtcggtcac gcgcacgcgc ggcccgcgcc gggcaggccc
     8581 ctgcccggca ccgaggccgt acgcaccccc cgtctccgga gccgacggcc cccgccccgc
     8641 gtgagcgcgg ggaggacgcc gtcggcccga agaacacggc cccgagcggg agacggcggc
     8701 acggtggcgg ggactcggcg aggccccgcc gctgtcacct gtgccagggc cccgtcaccg
     8761 cgaaggtcgt cccgggcgtg tagcagttga cgaacatcgt gtcgtagtcc ggggagaagc
     8821 agatgcccgc gaactcgccc cacgccggct cctcgggcgt tccgatgttc tggcggcccc
     8881 gcgccatcgg gtagacctcg ccgcgcgtgg tcacgccgaa gacgtgctgg gcgccctcgc
     8941 cgtcctccgc gaccatcagg ccgccgctgg gggcgaggca gatgttgtcg ggctcctcgc
     9001 ccgggagctg cacgtcggtg tcggggccga agacgacgac cagggtcagg cgccgccggg
     9061 cggggtcgta ccgccagatc tggccgtagt ggtcggcggc cgaaccctcg ttgctccgcg
     9121 cgaagctgga gacgaagtag accgaggagc caccccagta gcagccctcc agtttctgcg
     9181 cgtgggtggt gcccttcggg ccgaagtcct ggaaccggat cggcgtctcc gcggccagcg
     9241 ggtccggcac gtccacccac tccacccggt cgaaggccgc ccccgtctcc tggatcgccg
     9301 agaggtccgg cacccccggc acccgcatcg cctggagcga ccccccggcc cgcagcgagc
     9361 cgagcccgcc gcgcggcttg acgggcagga agcggtagaa gaggccgaac ggccgctcga
     9421 aggcgtcctc cgtctcgtag acgacgccgt tgcgcggatc gatcgcgacc gcctcgtgct
     9481 ggaagcggcc catcgcctcc agggggaccg cgccggtccg gtgcgggttc gtcgggtcga
     9541 cctcgaagat gaagccgtgg tccttggtgt agccgttggt cccggccttg tcctcggtct
     9601 cctcgcaggt cagccaggtg ccccacgggg tcggcccgcc cgcgcagttg gtcgaggtcc
     9661 ccgcgatggc gacccgctcg ccgaggaccc ggttcgcgga gtccagggtg agcgccgtac
     9721 agccgcccag cccctccggg tcgtaggtca gccccctcac ggccggtacg cggatctcgg
     9781 cgtcggtgcg gttctcgtgg ttgcggacga ggtggacctg gccccggcgc ccaccgaagg
     9841 cggccatccc gtcgtggttg ctcggcacct cgccctcacc ggagagcatc cggtcgccct
     9901 cgcgggacag cacccggtag cggaaacctt tcggcaggtc caggagaccc ttcgggtcgg
     9961 gcacgagcgg accgtacccg gctcgcccca tccccttcgc ggcggccgtg cccgcgaaga
    10021 gttcggtcag gctgccggtg aaagcgatac cggcgacggc accggtacga gcgaggactt
    10081 gacggcgtgt cgcggacatg ggcaactccc tgctgcggct ctgcggacag gaatgtgacc
    10141 cgcacgtgtg taccacgcac ccagccccac cggaaccagg cgtggcccgg gacaccgccg
    10201 tcacagccca cctggcctgc cgcgtacgcc tccggtaggg cagacggtcg ccacacccgg
    10261 ctcaccgcct cccggcgccg gcggactcga cggcgagagg cggacctggg ggcggccggc
    10321 gccgcggcca ccggacccct gaccggccgg cccggtggcc cctccaccta attcggaggc
    10381 gttcgcgcgc tgcccgtggt gtgcttctcc ggttccgcgc cggagttacc ggcggcaccc
    10441 gttccttcga caaggcattc ccatgcgatc ccagcacggc ccccgcgtcg cggtcgtcgg
    10501 cgccggcctc ggcggcctgg ccctcgctcg cgtactgcaa ctccacggcc gctccgtgac
    10561 ggtcctcgaa cgcgagcccg cccgtggcgc ccggcctcag ggaggcagcc tcgacatcca
    10621 ccccgacacc ggccagacgg cgctgcgggc ggccggactg ctggaccgtt tccgcgctct
    10681 gtcccggccg gagggccagg agtggcgcag ggtcgacccc gtgaccgccc tgcccctgcc
    10741 gggacccggc cacgacgagg agggccagcg ggagccgggg gacagcccgg agatcgaccg
    10801 cggccagttg cggggcctgc tcctggaatc cctcaccgag ggcaccgtcc ggtgggacag
    10861 cgccgtcacc ggggccaccc cgctcgggga cggcacctgg cgtctgcacc tcgccgacgg
    10921 cggtaccgag gacgccgatc tcgtggtcgg cgccgacggt gcctggtcac gcctccgccc
    10981 ggcgctctcc gacgcggtgc ccggctacac cggcgtcacc ttcgtggaga ccggcctgga
    11041 cgactgcgac atccgccatc cggccctcgc ccgcacggtc ggcgccggga cgctgctggc
    11101 cgcctccgac ggcaaggccc tgctcgccca gcgcaacggc gacggccaca tccgcgtcta
    11161 cgccgccttc cgcgccccgc gggactggcg gtcggccgcc ggtgtgcgcg ccgacgacgc
    11221 ggcggccgtc cgggcccgcc tgttgaagca cttcgacggc tgggacgagg gcctgctc
//
