LOCUS       NZ_CP045704            10399 bp    DNA     linear   CON 03-SEP-2024
DEFINITION  Streptomyces rutgersensis strain NBH77 chromosome, complete genome.
ACCESSION   NZ_CP045704
VERSION     NZ_CP045704.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces rutgersensis
  ORGANISM  Streptomyces rutgersensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces diastaticus group.
REFERENCE   1  (bases 1 to 6848830)
  AUTHORS   Benaud,N., Edwards,R.J., Amos,T.G., D'Agostino,P.M.,
            Gutierrez-Chavez,C., Montgomery,K., Nicetic,I. and Ferrari,B.C.
  TITLE     Antarctic desert soil bacteria exhibit high novel natural product
            potential, evaluated through long-read genome sequencing and
            comparative genomics
  JOURNAL   Environ Microbiol 23 (7), 3646-3664 (2021)
   PUBMED   33140504
REFERENCE   2  (bases 1 to 6848830)
  AUTHORS   Benaud,N., Edwards,R.J. and Ferrari,B.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2019) School of Biotechnology and Biomolecular
            Sciences, University of New South Wales, Botany St, Sydney, NSW
            2052, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 07-DEC-2018
            Assembly Method                   :: FALCON v. v 1.8.6
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014216335.1-RS_2024_09_03
            Annotation Date                   :: 09/03/2024 04:34:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,921
            CDSs (total)                      :: 5,831
            Genes (coding)                    :: 5,642
            CDSs (with protein)               :: 5,642
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 102 of 189
            Pseudo Genes (incomplete)         :: 130 of 189
            Pseudo Genes (internal stop)      :: 14 of 189
            Pseudo Genes (multiple problems)  :: 50 of 189
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:08:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1316159
            Orig. end                         :: 1326558
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP045704.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Belinda Ferrari, QAP Lab
            4002, Bioscience South E26, UNSW Sydney, Australia 2052 (Quarantine
            regulated isolate).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="5"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(270..287)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.25"
                     /tool="antismash"
     gene            complement(299..1390)
                     /locus_tag="F0345_RS05275"
                     /old_locus_tag="F0345_05315"
     CDS             complement(299..1390)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947418.1"
                     /locus_tag="F0345_RS05275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05315"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_100453222.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 7.7e-17, bitscore:
                     59.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEDMVLHVKGRILLSAQEVRDELWVVGGRITFTRPPGAREARTV
                     EGWALPGLVDAHCHVGLDRHGPVAAEVAEQQALTDREAGALLLRDAGSPSDTRWMDERD
                     DLPRVVRAGRHIARTRRYIRNYAHEIEPDELVAHVGREARRGDGWVKLVGDWIDRGVGD
                     LTPCWPREAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPDLAAGGEAKFPDWSAHMRRLYERRYDTVRAAWDAGIPVFVG
                     TDAGGSLPHGLVAAEVAELVTAGIPPLDALSATAWGAREWLGRPGLSEGAPGDLVVYAS
                     DPRADVRVLAAPRRVVIGGKVVA"
     gene            1465..2580
                     /locus_tag="F0345_RS05280"
                     /old_locus_tag="F0345_05320"
     CDS             1465..2580
                     /NRPS_PKS="Domain: Aminotran_5 (74-284). E-value: 6.5e-29.
                     Score: 92.9. Matches aSDomain:
                     nrpspksdomains_F0345_RS05280_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947419.1"
                     /locus_tag="F0345_RS05280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05320"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_100453221.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.9e-22, bitscore:
                     76.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLTPLTAAHFANIERRVAALLSTSQDVVIAQGEALLPLE
                     GCIRGVAGPDTTALNVITGPYGQTFGDWLRDCGARVVDLAVPYDAAARAAQVAEALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGRVVREHGALLMVDAVASVGAEPLLPDAWGVDLCV
                     VGAQKAMGGPAGVSAVSVSERAWQRMAANPGAPRRSYLSLLDWKERWIDAGRTALLHAP
                     AQLEMLALQACVERIEDEGLETVIGRHARAAAATRAGVTAIGGGFAPYVREARDAAPVA
                     TTLRAPEGVDASELVARALAADPVLPLVAGGGALAKEMIRVNHYGTAAAGGTVHSCLAA
                     LGAALSDLTGRPVDLDAARRAATAAWEHPAA"
     aSDomain        1687..2316
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F0345_RS05280_Aminotran_5.1"
                     /evalue="6.50E-29"
                     /label="F0345_RS05280_Aminotran_5.1"
                     /locus_tag="F0345_RS05280"
                     /protein_end="284"
                     /protein_start="74"
                     /score="92.9"
                     /tool="antismash"
                     /translation="RDCGARVVDLAVPYDAAARAAQVAEALAEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGRVVREHGALLMVDAVASVGAEPLLPDAWGVDLCVVGAQKAMGGPAGVSA
                     VSVSERAWQRMAANPGAPRRSYLSLLDWKERWIDAGRTALLHAPAQLEMLALQACVERI
                     EDEGLETVIGRHARAAAATRAGVTAIGGGFAPYVREARDAAPVATTL"
     misc_feature    2708..2721
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.34"
                     /tool="antismash"
     misc_feature    complement(2851..2866)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2861..2876
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2896..2909
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.62"
                     /tool="antismash"
     misc_feature    2900..2918
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.45"
                     /tool="antismash"
     misc_feature    2920..2935
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.91"
                     /tool="antismash"
     misc_feature    2973..2983
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
     misc_feature    2995..3007
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    2999..3013
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.45"
                     /tool="antismash"
     misc_feature    complement(3007..3022)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    3021..3033
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3032..3580
                     /gene="ectA"
                     /locus_tag="F0345_RS05285"
                     /old_locus_tag="F0345_05325"
     CDS             3032..3580
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947420.1"
                     /locus_tag="F0345_RS05285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05325"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_185392892.1"
                     /transl_table=11
                     /translation="MTAAQADLARGRSTLKEMPEDLRLDHPGVEDGAAIWRIARDSRTL
                     DLNSSYSYLLWCRDFAGTSVVARDAGGEPVGFVTGYFRPERPHTLVVWQVAVDASQRGR
                     GLAAALLDGLTDRLREKGRLDGIETTITPGNTPSERLFTSYAKRHGAELEREVLFGAGL
                     FPDGGHDPEVLYRIGPFAD"
     gene            3695..4966
                     /gene="ectB"
                     /locus_tag="F0345_RS05290"
                     /old_locus_tag="F0345_05330"
     CDS             3695..4966
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 8.5e-76.
                     Score: 247.1. Matches aSDomain:
                     nrpspksdomains_F0345_RS05290_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="F0345_RS05290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05330"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_100453219.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.6e-80, bitscore:
                     268.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSYLYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGITHGLDMGTTAKRAFLETFQNVLLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVQDFLWFERLLEDQGSGLNTPAAVIVETIQGEGGINVARP
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGPFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWSDGQMEKQTLARGEQVEQALT
                     AILDEHGSEAIASIRGRGLVWGMEFTDKTRAGAVSKRAFELGLLVETSGPESEVVKLLP
                     PLTVSPDELDEGLRILARAVRETA"
     aSDomain        3779..4792
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F0345_RS05290_Aminotran_3.1"
                     /evalue="8.50E-76"
                     /label="F0345_RS05290_Aminotran_3.1"
                     /locus_tag="F0345_RS05290"
                     /protein_end="366"
                     /protein_start="28"
                     /score="247.1"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGITHGLDMGTTAKRAFLETFQNVLLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVQDFLWFER
                     LLEDQGSGLNTPAAVIVETIQGEGGINVARPEWLRALADLCKRRDMLLIVDDIQMGCGR
                     TGPFFSFEEAGITPDIVTLSKSIGGYGMPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWSDGQMEKQTLARGEQVEQALTAILDEHGSEAIASIRGRGLVWGMEFT"
     gene            5001..5399
                     /locus_tag="F0345_RS05295"
                     /old_locus_tag="F0345_05335"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947422.1"
                     /locus_tag="F0345_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05335"
                     /product="ectoine synthase"
                     /protein_id="WP_003947422.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.3e-55, bitscore:
                     182.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETDMWYANHIEAVLCVEGEAELTNRETGETHWIEPGTMYLLNGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEED"
     gene            5403..6299
                     /gene="thpD"
                     /locus_tag="F0345_RS05300"
                     /old_locus_tag="F0345_05340"
     CDS             5403..6299
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637026.1"
                     /locus_tag="F0345_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05340"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_100453218.1"
                     /sec_met_domain="PhyH (E-value: 1.7e-37, bitscore: 128.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVASERTDLYPSRTATEVTIPRKDPVVWSPQDAPGPITRSDLQS
                     YDENGFLAIEELITPEEVGVYRAELDRLVADPLIRADERAVVEPKSEKVRSVFEIHKIS
                     EVFAGLVRDERLLGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPR
                     MRAVSVSIALTENYDTNGGLMIMPGSHRTYVGCNGVTPKDNYKRSLQMQDAGIPSDEVL
                     TEFADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAEEPFAA
                     PVRRPEFIGARDFTPVR"
     gene            complement(6843..7892)
                     /locus_tag="F0345_RS05305"
                     /old_locus_tag="F0345_05345"
     CDS             complement(6843..7892)
                     /NRPS_PKS="Domain: Aminotran_5 (52-343). E-value: 2e-25.
                     Score: 81.4. Matches aSDomain:
                     nrpspksdomains_F0345_RS05305_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895195.1"
                     /locus_tag="F0345_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05345"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_100453392.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.9e-19, bitscore:
                     68.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLAHSLAEDEFEPALTHLATASAGLLPLSAARAVHEAVDASAAG
                     APPDMFGAMEAARASFARLVGVSAERVATGSSVATFTGLIASSLPDGAEVLTAEGDFSS
                     LVNPFHVRPGLTVRTVPLERIAEHVTEATALVAVSAVQSADGRRADLAAVREAARAHGA
                     RTLIDASQAAGWDTLDADAYDYTVAVGFKWLLCPKGTAFLTVPAGLGAPPPLLAGWVAG
                     RLPYESCYGPVTEPARSARRYDESPALFSWAGAPQALALLERVGTEAVGAHDVALAERF
                     RERLTRAGHAPVPAPGSAIVSVPGLGERAKALGERGITVSDRAGMLRAAFHLYNTESDV
                     DRLAAALGG"
     aSDomain        complement(6864..7736)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F0345_RS05305_Aminotran_5.1"
                     /evalue="2.00E-25"
                     /label="F0345_RS05305_Aminotran_5.1"
                     /locus_tag="F0345_RS05305"
                     /protein_end="343"
                     /protein_start="52"
                     /score="81.4"
                     /tool="antismash"
                     /translation="AMEAARASFARLVGVSAERVATGSSVATFTGLIASSLPDGAEVLT
                     AEGDFSSLVNPFHVRPGLTVRTVPLERIAEHVTEATALVAVSAVQSADGRRADLAAVRE
                     AARAHGARTLIDASQAAGWDTLDADAYDYTVAVGFKWLLCPKGTAFLTVPAGLGAPPPL
                     LAGWVAGRLPYESCYGPVTEPARSARRYDESPALFSWAGAPQALALLERVGTEAVGAHD
                     VALAERFRERLTRAGHAPVPAPGSAIVSVPGLGERAKALGERGITVSDRAGMLRAAFHL
                     YNTESDVDRL"
     gene            complement(8017..8742)
                     /locus_tag="F0345_RS05310"
                     /old_locus_tag="F0345_05350"
     CDS             complement(8017..8742)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412186.1"
                     /locus_tag="F0345_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="F0345_05350"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_100453217.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFDKALAAFPHRDRVEVVHRSYELDP
                     ARAKGDTELVLPMLARKYGMSEAQAAEAERNLGQNAAAEGLPYLTEGRDHGNTFDMHRL
                     LHLARARGRQDALLAVLYRANFAERESVFGDDERLVALAVEAGLAEAEAREVLADEQRY
                     AAEVRADERAATELGANGVPFFVLDRRYGVSGAQPADVFTQALTQAWESHVPAAGLATP
                     AGAEGGAACGPDGCEVPEG"
ORIGIN
        1 ccacagagac atggagccgt cgagggccgc gcttggggac aggcggccgc cgacgacgtc
       61 gttgccggaa tctttacgca ctgtgggtga atgggtgacg acctggagtg agttcactcc
      121 aaaggggggt tttgacgcag atgttcgaac cgaccggcac ggccgttccc tcgacgtcac
      181 cctccacgcc acctcgcggc ccccggaggg cgtacgcggg cgcgggtcct tcatcggggc
      241 cggagcacaa cgacgccgcc ccggccccgg taacggcccg tcaacacctc acgcgggctc
      301 aggcgacgac cttgccgccg atcaccaccc ggcgcggcgc cgccagcacc cggacgtcgg
      361 cgcgcgggtc gctcgcgtag accaccaggt cgcccggggc gccctcggac agtcccgggc
      421 ggccgagcca ttcgcgggca ccccacgccg tggcggagag cgcgtcgagc ggcgggatgc
      481 cggcggtcac cagttcggcg acctcggcgg cgaccaggcc gtgcgggagg ctgccgcccg
      541 cgtcggtgcc gacgaagacg gggatgccgg cgtcccaggc ggcgcggacc gtgtcgtacc
      601 ggcgctcgta cagccgtcgc atgtgggccg accagtcggg gaacttcgcc tcgccgcccg
      661 ccgcgaggtc ggggaaggtg gcgatgttga ccagggtcgg cacgatggcg accccgcgtt
      721 cggcgaagag cgggatggtg tcctcggtca ggccggtggc gtgctcgacg cagtcgatgc
      781 ccgcctcgac caggtcgcgc agcgagtcct cggcgaagca gtgcgcggtg acccgggcgc
      841 cgagccggtg cgcctcggcg atggccgcct cgaccgcctc acggggccag cagggcgtca
      901 ggtcccccac gccccggtcg atccagtcgc cgaccagctt gacccagccg tcgccgcgcc
      961 gcgcctcccg gccgacgtgg gcgaccagtt cgtccggctc gatctcgtgg gcgtagttgc
     1021 ggatgtagcg gcgggtgcgg gcgatgtggc ggccggcccg gacgacccgg ggcagatcgt
     1081 cgcgttcgtc catccagcgg gtgtcgctcg gggaacccgc gtcgcgcagc agcagtgccc
     1141 cggcctcccg gtcggtgagc gcctgctgct ccgcgacctc ggccgcgacc gggccgtgcc
     1201 ggtccaggcc gacgtggcag tgcgcgtcga ccaggccggg cagcgcccac ccctcgacgg
     1261 tgcgcgcctc gcgggcgccc ggcggccggg tgaaggtgat ccggccgccc accacccaga
     1321 gttcgtccct gacctcctgc gcggagagca gtatccggcc cttcacgtgc agcaccatgt
     1381 cttcgctcat gcacggcagc gtaggacccg gtgggtacgc tcggaccggc agcccgacga
     1441 tcgaccggcg aagagagcac caccgtgacg caccccctcc tggacctgac cccgctgacc
     1501 gccgcgcatt tcgcgaacat cgagcgccgc gtcgcggcac tgctctccac ctcgcaggac
     1561 gtcgtcatcg cccagggcga ggcgctgctg ccgctggagg ggtgcatccg cggcgtggcg
     1621 ggcccggaca ccaccgccct caacgtcatc accggcccgt acgggcagac cttcggcgac
     1681 tggctgcgcg actgcggggc gcgggtcgtc gacctggcgg tgccgtacga cgccgcggcc
     1741 cgcgccgccc aggtggccga ggcgctggcg gagcaccccg agatcgactt cgtctcgctg
     1801 gtgcacgccg aggcggcgac cgggaacacc aacccggtgg cggagatcgg ccgggtggtg
     1861 cgggagcacg gcgcgctcct catggtggac gcggtggcgt cggtcggtgc cgagccgctc
     1921 ctcccggacg cctggggggt ggacctctgc gtcgtcggag cccagaaggc gatgggcggc
     1981 cccgccgggg tctcggcggt ctcggtcagc gagcgggcct ggcagcggat ggcggccaac
     2041 ccgggggcgc cgcgccgttc gtacctctcg ctgctggact ggaaggagcg gtggatcgac
     2101 gccgggcgca ccgccctgct gcacgccccc gcccagctgg agatgctggc cctccaggcg
     2161 tgcgtggagc ggatcgagga cgagggcctg gagacggtga tcggccggca cgcgcgggcg
     2221 gcggcggcca cccgggcggg cgtgacggcg atcggcggcg gtttcgcgcc ctacgtccgc
     2281 gaggcgcggg acgcggcgcc ggtggcgacc acgctgcggg cgccggaggg cgtcgacgcc
     2341 tcggagctgg tggcgcgggc gctggcggcg gacccggtgc tgccgctggt ggccggcggc
     2401 ggggcgctgg ccaaggagat gatccgggtc aaccactacg gcacggccgc ggccggcggc
     2461 acggtccact cctgcctggc cgcgctgggc gcggcgctga gcgacctcac cggccgcccg
     2521 gtcgacctgg acgccgcgcg gcgggcggcg accgcggcct gggagcaccc cgcggcctga
     2581 ccctccgcac cggcccggcg ggccgtccgg tgcgcggcga ctgccgtgcg cggggcggcc
     2641 cgttccgtct ctccggattc ccggacacgc cgaagtccgc accgacttcg gcgcggacgc
     2701 ggatcgatat tcttatcagc agacctattg cagggaattc cgaattcttt cgcactcacc
     2761 ttcccctatt cgtctgcccg cttcctccga agctaaacgc atcgattttc cggacaccga
     2821 aaccgcgtaa ttcggacgcg gctcacatgc gttacgcact tgtgactgac tccacacggt
     2881 gaccgtttcg cgccttatat ccagtacaaa tcaccatctt cggacacctg ttcgcgcagg
     2941 cgcgccctcg cgcccgataa cacagaaagc gctgccatgt aaccctgacc cgcgatgcat
     3001 ttttcagatt cgatgggtaa attcaatttg catgaccgcc gcacaagccg accttgcacg
     3061 tggccgatcc accctgaagg aaatgcccga ggatttgcgc ctggaccacc cgggcgtcga
     3121 ggacggagca gccatctggc gtatcgcccg tgactcgcgc accctcgacc tcaactcctc
     3181 ctacagctac ctcctgtggt gtcgcgactt cgccggcacc tccgtggtgg cccgcgacgc
     3241 cggcggcgag ccggtcgggt tcgtgaccgg gtacttccgg cccgagcgcc cgcacaccct
     3301 cgtcgtctgg caggtcgccg tggacgcctc gcagcgcgga cgcggcctcg ccgccgccct
     3361 gctggacggg ctcaccgacc ggctccgtga gaaggggcgg ctggacggga tcgagaccac
     3421 catcacgccg gggaacacgc cctcagaacg cctcttcacc tcctacgcca agcgccacgg
     3481 cgccgagctg gagcgcgagg tcctcttcgg tgccggcctc ttccccgacg gggggcacga
     3541 ccccgaggtg ctgtaccgca tcggaccgtt cgccgactga caccggaacg ccgcacagca
     3601 acccaggtcc ggccgggaag caagcccccg gtcgcccttc cgcacgtatc cctgacttcc
     3661 gccgcttcac cccccatctc ccaggagagc agtcgtgacc atcaccccgc ccgccctgag
     3721 cgtcttcgag acccttgagt cggaggtacg gagctactgc cgcagctggc ccgccgtctt
     3781 cgaccgggcc cagggcagct acctgtacga cgaggacggc cacacctacc tcgacttctt
     3841 cgccggcgcc ggcgccctca actacggcca caacaacccg gtgctgaaac gcgcgctgat
     3901 cgactacatc gagcgcgacg gcatcaccca cggcctggac atgggcacca ccgccaagcg
     3961 cgcgttcctg gagaccttcc agaacgtgct gctgcgcccg cgcgacctgc cgtacaaggt
     4021 catgttcccc ggcccgacgg gtaccaacgc cgtcgagtcg gcgctgaagc tggcccgcaa
     4081 ggtcaagggc cgcgagtcga tcgtctcctt caccaacgcc ttccacggca tgtcgctcgg
     4141 ctcgctcgcc gtgaccggca acgccttcaa gcgcgccggc gccggcatcc cgctggtgca
     4201 cggcacgccg atgccgttcg acaactactt cgacggcacc gtgcaggact tcctgtggtt
     4261 cgagcggctg ctggaggacc agggctcggg tctcaacacc ccggccgcgg tcatcgtcga
     4321 gaccatccag ggcgagggcg gcatcaacgt ggcccggccc gagtggctgc gcgccctggc
     4381 cgacctctgc aagcgccgcg acatgctgct gatcgtcgac gacatccaga tgggctgcgg
     4441 ccgcaccggc ccgttcttct ccttcgagga ggcgggcatc accccggaca tcgtcaccct
     4501 gtcgaagtcc atcggcggct acggcatgcc gatgtcgctc tgcctgttca agccggagct
     4561 ggacatctgg gagccgggcg agcacaacgg caccttccgg ggcaacaacc cggccttcgt
     4621 caccgccgcc gccgcgctcg acgcgtactg gagcgacggc cagatggaga agcagaccct
     4681 ggcccggggc gaacaggtcg agcaggcgct caccgcgatc ctcgacgagc acggcagcga
     4741 ggccatcgcc tccatccgtg gccggggcct ggtctggggc atggagttca ccgacaagac
     4801 ccgcgccggc gccgtctcca agcgcgcctt cgagctgggc ctgctggtcg agacttccgg
     4861 gccggagagc gaagtggtca agctgctgcc gccgctcacc gtgagcccgg acgagctgga
     4921 cgagggtctg cgcatcctcg cccgcgccgt ccgagagacg gcctgacaca caccgacgga
     4981 agaaggggca cgaccgcact gtgatcgtcc gatcgttcaa ggacatcgag aacaccgacc
     5041 ggcacgtcaa atcggcgtcc ggcacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtcggctt ctcgttgcac gagaccatcc tctacgcggg cacggagacc gacatgtggt
     5161 acgccaacca catcgaggcc gtgctctgcg tggagggtga ggccgagctg accaaccgcg
     5221 agacgggcga gacccactgg atcgagcccg gcacgatgta cttgctgaat ggccacgaga
     5281 agcacaccct gaggccgaag acagattttc gctgcgtctg cgtcttcaac cctcccgtga
     5341 ccgggcggga ggaccacgac gagaacggcg tctacccgct gctgaccgag gaggactgag
     5401 tcatgactgt cgcgtcggag cgcaccgatc tctacccgag ccggactgca accgaggtga
     5461 cgatcccgcg caaggacccc gtggtgtggt ccccgcagga cgcccctgga cccatcacgc
     5521 ggtccgacct gcagtcttac gacgagaatg gcttcctcgc catcgaggag ctgatcaccc
     5581 ctgaggaagt cggggtctac cgggccgagc tggaccggct ggtcgccgac ccgctcatcc
     5641 gggccgacga gcgcgccgtc gtcgagccca agtcggagaa ggtccgctcg gtcttcgaga
     5701 tccacaagat cagtgaggtc ttcgccgggc tggtccgcga cgagcggctc cttggccggg
     5761 cccggcagat cctcggctcg gacgtctacg tccaccagtc gcggatcaac gtcaagccgg
     5821 gcttcggggc atccgggttc tactggcact cggacttcga gacctggcac gccgaggacg
     5881 gcctgcccag gatgcgcgcg gtgtccgtct cgatcgcgct gaccgagaac tacgacacca
     5941 acggcggcct catgatcatg ccggggtcgc accgcaccta cgtggggtgc aacggcgtca
     6001 cgccgaagga caactacaag cggtcgctcc agatgcagga cgcgggcatc ccctccgacg
     6061 aggtgctgac cgagttcgcc gaccggcacg gcatccggct gttcaccggc aaggccgggt
     6121 cggccacctg gttcgactgc aacgccatgc acggttccgg ggacaacatc accccgtacc
     6181 cgcgcagcaa cgtgttcatc gtcttcaaca gcgtcgagaa cgctgctgag gagcccttcg
     6241 cggcgcccgt ccggcgcccg gagttcatcg gggcgcgtga cttcactccg gtgaggtgag
     6301 accggtgacg cccgcttcac ccgagtggag caggcggacc ggatgagtcg ctgaccgcgg
     6361 ctcgtcacgc accggcgcac acggcgcccg ggccacggga ccgggggcgg ccacaggcgg
     6421 gccgctcccg gtccggttgc gtcccggggt ctttccggcc ggccggcacc gcgcgcgagc
     6481 gcgccggtca gccggtcagc tccgggtagt cggtgtggtc ctgggcgccg tcgccgtaca
     6541 tcgttgggga aaggcccggg cagggcggcc ctggcacgga gcacggtccg taccgcgccg
     6601 gtcgtgacgg tgcggtcgga gaacgcgcgc gaggtgggct gccaggaggc cggggcgcct
     6661 ggcggctcgt cgcgggcgag ggggcgtgcc gccccggctc agcacggtcc cgcgctgccg
     6721 ccgtaccccg gtcccggccc caccacgtca ggggaccccc gtgcctcgcc cccggcaccc
     6781 acgaccccac gcctcggcag gtccgccccc gcgctcccgg cgcccgccct gcccccgtgg
     6841 cctcagccgc ccagtgccgc cgccagccgg tccacgtcgc tctcggtgtt gtagaggtgg
     6901 aaggcggccc ggagcatccc ggcccggtcg gagaccgtga tcccccgctc cccgagggcc
     6961 ttggcgcgct cgccgagccc cggcacggag acgatggccg agccgggggc cggcacgggg
     7021 gcgtgtcctg cgcgggtcag ccgctcccgg aaccgctcgg cgagcgcgac gtcatgggcg
     7081 cccaccgcct cggtcccgac ccgctccagc agggcgaggg cctgcggcgc gcccgcccag
     7141 gagaacagcg cggggctctc gtcgtagcgg cgcgcgctgc gggccggttc ggtgacgggc
     7201 ccgtagcagc tctcgtacgg gagccggccg gcgacccagc cggcgagcag cgggggcggg
     7261 gcgccgaggc cggcggggac ggtgaggaag gcggtgccct tcgggcagag cagccatttg
     7321 aaaccgacgg cgacggtgta gtcgtacgcg tcggcgtcga gggtgtccca gccggcggcc
     7381 tgcgaggcgt cgatcagggt gcgggcgccg tgggcgcggg ccgcctcgcg gacggcggcg
     7441 aggtcggcgc ggcggccgtc ggcggactgc acggcgctga ccgcgaccag cgcggtggcc
     7501 tcggtgacgt gttcggcgat ccgctccagc ggcacggtgc gcaccgtcag gccgggccgc
     7561 acgtggaagg ggttgaccag cgagctgaag tcgccctcgg cggtcagcac ctcggcgcca
     7621 tcgggcagcg aggaggcgat caggccggtg aaggtggcga cggacgagcc ggtggcgacc
     7681 cgctcggcgg agacgcccac gagccgggcg aaggaggcgc gggcggcctc catggcgccg
     7741 aacatgtcgg gcggggcgcc cgctgcggag gcgtcgacgg cctcgtgcac cgcccgggcc
     7801 gcgctcagcg gcagcaggcc ggcactggcg gtcgcgagat gggtcagggc cggctcgaac
     7861 tcgtcctcgg ccaggctgtg agcgagatcc atgcggccac tgtgcaccgc cgtgcggcgg
     7921 ccgtccatgg tgcggtggcc gccgaagcgt ccgcgccccg gctccggccc gcctcccgcc
     7981 gaagccggtg gcgggccggg cgccggcgtc ccgctctcag ccctcgggga cctcgcagcc
     8041 gtcggggccg cacgccgcgc cgccctcggc gccggccggg gtggcgagcc cggcggccgg
     8101 gacgtggctc tcccacgcct gggtgagcgc ctgcgtgaag acgtcggcgg gctgggcacc
     8161 ggaaacgccg taccgccggt cgaggacgaa gaacggcacg ccgttggcgc ccagttcggt
     8221 ggcggcgcgc tcgtcggcgc ggacctcggc ggcgtaccgc tgctcgtcgg cgaggacctc
     8281 gcgggcctcg gcctccgcca gcccggcctc gacggcgagg gcgacaagcc gctcgtcgtc
     8341 gccgaagacg ctttcccgct ccgcgaagtt ggcgcggtac aacacggcga gcagggcgtc
     8401 ctggcggccc cgggccctgg cgaggtggag caggcggtgc atgtcgaagg tgttgccgtg
     8461 gtcgcggccc tcggtgaggt agggcaggcc ctcggcggcg gcgttctgcc cgaggttccg
     8521 ctcggcctcg gcggcctgcg cctcgctcat gccgtacttc cgggcgagca tcggcaggac
     8581 cagctcggtg tcgcccttgg cgcgggccgg gtccagctcg taggagcggt gcacgacctc
     8641 gacccggtcg cggtgcggga aggcggcgag cgccttgtcg aaccgcgcct tccccacgta
     8701 gcaccagggg caggcgatgt cggaccagat ctcgacgcgc atggctgcgg ttcaactcct
     8761 cggcccggcg gaccgggcgg gtcgggggtg gcgggagaag gctcccgggc gtgcgcccac
     8821 cagggtacgg agggcgcctc cgctctctgg cgacacgggg ccggacctgt gcattccgca
     8881 ccgcgtcccg ctggggcccc gcctccctca ggcgccgttc tcggcccggc cgtcgacgtg
     8941 ccggggcagc cccagcgggt tgccgtcccg cagttccggc ggaagcagcg cctggggcgt
     9001 cgtctggtag gcgaccgggc gcagccagcg ttccacggcg gcggtgccga ccgaggtggc
     9061 cgtggaggtc gccgccgggt aggggccgcc gtggtgctgg gcgtcggcga ccgcgacccc
     9121 ggtcggccag ccgccgacga cgacccggcc ggccagccgg gtgagccgct ccagcaacgc
     9181 ggccgcaccg ccttcgccgc gctcctcggc gccggacagg tggagggtgg cggtgaggtt
     9241 gccgggcagc cgggcgagga gctccccggc ctcggcgggg ccggcgtagc gggcgaggac
     9301 ggtgaccggc ccgaagcact cctccaggag caggtcgtgc ggccccgggg cggcgagccg
     9361 gccggcggcg acggtcagaa agcccgcggc cacggtgtgc tcccccgccg cccccggcgt
     9421 caccgggctc tcgaccccgt ccagcgcggc ccgctcgccg accccggcga ggaaggcgtc
     9481 gcggatgcgg gtgtcgagga ggaccccggc ctcggtgtcg ctgacggccg ccgtcagcgc
     9541 ggtgaccagc cggtcgccgg cctcgcccgc cgggaccagc accagcccgg gcttgacgca
     9601 gaactggccc gtccccagcg tcaccgaggc cgccagcccc cgggcgacct cctcggcccg
     9661 ctcggccgcc gcgcccgggg tgacgaggac cgggttgagg ctgcccagtt caccgtggaa
     9721 ggggatgggc acgggccggg ccgccgcggc gtcgaacagg gcgcgcccgc cccgtacgga
     9781 cccggtgaag ccggcggcgg tgacgagcgg gtggccgatc agggccacgc ccgcgtcgaa
     9841 gccgtgcacg agcccgaggc actccggcgg tacgccggtg tcggcggcgg cccggcgcag
     9901 cagcgcgaga gtcagctcgg aggtggccgg gtggtccggg tgggccttga tcacgacggg
     9961 gcagcccgcc gccagcgcgc tggcggtgtc gccgccgggc gcggagaagg cgaaggggaa
    10021 gttccccgcc gcgtagacgg cgaccacacc gagcggtatc cgcatgcgcc gcaggtccgg
    10081 gacgggtggc acggcggccg ggtcggcgtg gtcgatccgc gcgtcgagga acgcgccctc
    10141 ttcggcgagg gcggcgaagc tccgcaactg gccggtcgtc cgggtcagtt cgccgtcgag
    10201 ccggaccggt ccgagcgccg tctcggcgtc ggcggcctcc accaggtggg cccgggcgcc
    10261 gtcgaggcgg tccgccgccg cccgcagcag cgcggcgcgt acctcccggt cggcgagggc
    10321 gctccgtgcg gcgtgggccg cgcgcaccgc ctggtccacc tcggccccgc tcgcctccac
    10381 ggcgacctgc tcacgctcc
//
