LOCUS       NZ_JACHJQ010000008     12691 bp    DNA     linear   CON 27-SEP-2024
DEFINITION  Actinophytocola algeriensis strain CECT 8960 Ga0436956_08, whole
            genome shotgun sequence.
ACCESSION   NZ_JACHJQ010000008
VERSION     NZ_JACHJQ010000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinophytocola algeriensis
  ORGANISM  Actinophytocola algeriensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 508091)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 508091)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 154.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203735.1-RS_2024_09_27
            Annotation Date                   :: 09/27/2024 05:42:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,095
            CDSs (total)                      :: 9,030
            Genes (coding)                    :: 8,881
            CDSs (with protein)               :: 8,881
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2, 2 (5S, 16S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 44 of 149
            Pseudo Genes (incomplete)         :: 120 of 149
            Pseudo Genes (internal stop)      :: 3 of 149
            Pseudo Genes (multiple problems)  :: 17 of 149
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 424979
            Orig. end                         :: 437670
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJQ010000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12691
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="4"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12691
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12691
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7691](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7691)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    324..336
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            complement(363..1547)
                     /locus_tag="FHR82_RS35580"
                     /old_locus_tag="FHR82_007244"
     CDS             complement(363..1547)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014081.5"
                     /locus_tag="FHR82_RS35580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007244"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_184814990.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.3e-47, bitscore:
                     157.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLAIINAAVFDGESAELVDGPVLVEDGRISAIGGSGAADRVLDA
                     RGGTVLPGLIDAHCHAYGVSLDLLSIEGAPLSYLALTGARRLKAALARGFTTVRDVAGG
                     DPGIARAIAEGTIPAPRYLYTGAALSQTGGHGDARGVDSDVCGCHVHMVEVVDGVDAVR
                     AAARDRFRRGAHAIKMMTSGGVMSLADPIRIPQYSAEEIRAVTDEAARRGSYVAAHAYS
                     AEAIRHSVENGVRSIEHGNLMDAETAALMASRGAFLVPTLATYDAISRRGADNGLAPVS
                     QEKNLEVLHAGANAIELARAAGVAIGFGTDLMGDLDDEQLNGLRLQSEIDGVLNALRSA
                     TSVNADLIGRPDLGRVQVGAVADLVIVDGDPFTEPAVLWGPRRTVVQAGVVVGE"
     gene            1701..2402
                     /locus_tag="FHR82_RS35585"
                     /old_locus_tag="FHR82_007245"
     CDS             1701..2402
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807817.1"
                     /locus_tag="FHR82_RS35585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007245"
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="WP_184815102.1"
                     /transl_table=11
                     /translation="MFRVLDLKLDVRGAEHIPASGGAVLACTHVSYLDFIFCGLAGLPS
                     GRKTRFMAKEQIFRNRIAGPLMRGMRHISVDRAAGQASYQEAVAKLRAGEVVGVFPEAT
                     ISRSFTVKPIKTGAVRMAEEAGVPVIPMTVWGSQRLWTKGRPKNLTQRHVPVLIRAGDP
                     FHPGKDDDLEALTADLRERMSALLDQAQADYPEKPATKEFWLPAHLGGSAPTPEEAAAL
                     DERPRGSKPTT"
     gene            complement(2463..3119)
                     /locus_tag="FHR82_RS35590"
                     /old_locus_tag="FHR82_007246"
     CDS             complement(2463..3119)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237161.1"
                     /locus_tag="FHR82_RS35590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007246"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_184814991.1"
                     /transl_table=11
                     /translation="MTDSFAALGVRRNLREEITQTLRAAVISGELRPGVVYSAPSLAAQ
                     FGVSATPVREAMLDLASEGLVDSVRNKGFRVTSVSSKDLDELSELRALIEVPTVARITA
                     AGVAPSVLESLRPLAATIEEAAAAGDLITHVAADMEFHLRLLAVGGNSHLVETVRSLRS
                     RSRIYGLRKLADQGELVPSAREHAALLDLIEAGDVDGAERLMDRHIRHVRGIWAE"
     gene            complement(3116..4117)
                     /locus_tag="FHR82_RS35595"
                     /old_locus_tag="FHR82_007247"
     CDS             complement(3116..4117)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646747.1"
                     /locus_tag="FHR82_RS35595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007247"
                     /product="proline racemase family protein"
                     /protein_id="WP_184814992.1"
                     /transl_table=11
                     /translation="MRAKRVFHAVDSHTEGMPTRVITGGVGVIPGDTMSARREYFMAHL
                     DHVRQLLMYEPRGHSAMSGAILQPPAREDADWGVVFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVPVAEPVTTVRLDTPAGLVVADVAVSDGVARSVTIRNVPSFSVGLDREVPVPGFGP
                     VRYDMAFGGNFYAILGVEQLGLPFSREEGPRLLAAGLSIMDAINSSDRPVHPVDPSIAG
                     CHHVYLAAPGSDARHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLDTEFRNES
                     FIGTRFTGRLVASTSVGDVPAVVPEITGRAWITGTAQYFLDPDDPFPQGFQL"
     gene            complement(4117..5004)
                     /locus_tag="FHR82_RS35600"
                     /old_locus_tag="FHR82_007248"
     CDS             complement(4117..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658768.1"
                     /locus_tag="FHR82_RS35600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007248"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_184814993.1"
                     /transl_table=11
                     /translation="MSKPWHGVLVATALPMTDRGEVDFDRFAEHVAWLAANGCHGVTPN
                     GSLGEYPTLTVEERARVVRTAVEAAPDGFTVMPGTGAYGSAESRRWAEQAAEAGCPAVM
                     SLPPNAYRGDERAVLAHYREVAKAGVPVVAYNNPIDTKIDLVPELLAELYQEGLIVGVK
                     EFTGDVRRPYRIAELAPGLDVLCGADDVALELAVAGSPGWIAGFPNSLPKSTVEFWQAA
                     AESDLATALPLYRVLHPLLRWDSRVEFVQAIKLSMDIVGRYGGPCRQPRVPLTPEHEAA
                     VRQATERALAEGLS"
     gene            complement(5001..6302)
                     /locus_tag="FHR82_RS47750"
                     /old_locus_tag="FHR82_007249"
     CDS             complement(5001..6302)
                     /EC_number="1.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945562.1"
                     /locus_tag="FHR82_RS47750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007249"
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="WP_311771431.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.3e-22, bitscore:
                     75.1, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 9.6e-10, bitscore:
                     33.5, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSPADIAVIGAGPAGLAAAAAARGRGARVALLDSAGRPGGQYW
                     RHRDGHNPIPASLRQDILAGHAVWHVEVEADGFVLHTTAGEVRARTVIVATGAYDRQLP
                     FPGWTLPGVFTAGGAQAMLKGHGVPVGDRVVVAGTGPFLLPVAAALRHRVTGVFEAGSP
                     LGFARHPLAVARNPGKLVEGARYLRALHGHRIPFHTRTAVVAAHGDDRVHAVTVARLDR
                     NRRPVSEREIECDAVAVGYGFTPQLELPLQLGCDTRLDADGSLVATADEWQRASVPGVY
                     LAGEVCGVGGAQLSVVEGAIAGAHAAGARPRRSLLRKRAALRAFAAAMHDTYRVGAGRP
                     ADDTIVCRCEEVTVREVRESVVDLGATDPRGVKLLARPGMGLCQGRVCGYATACLVAEA
                     CGRPVTAADLRGLAARPIAAPVTLGNLAEGVEDA"
     gene            complement(6283..6552)
                     /locus_tag="FHR82_RS35610"
                     /old_locus_tag="FHR82_007250"
     CDS             complement(6283..6552)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658766.1"
                     /locus_tag="FHR82_RS35610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007250"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_184814994.1"
                     /sec_met_domain="Fer2_4 (E-value: 7.4e-29, bitscore: 94.4,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTFSFDGEEVTAEPGQSVGAALLAAGYRSWRTTRHGGAPRGLFC
                     GIGVCFDCLVVVNGRPGLRACLTEVRDGDVVSSQEGAGRDHLAC"
     gene            complement(6549..7691)
                     /locus_tag="FHR82_RS35615"
                     /old_locus_tag="FHR82_007251"
     CDS             complement(6549..7691)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454770.1"
                     /locus_tag="FHR82_RS35615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007251"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_311771432.1"
                     /sec_met_domain="DAO (E-value: 1.1e-65, bitscore: 217.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVDVLVIGAGMVGAACAYHCAAAGLRVTVLDRGGVATGTTSGGE
                     GNILVSDKEPGPELDLALLSVRRWRILGERIGADRLELEHKGGVVVARSAPALAGLADL
                     TGRQRACGVDAVDVTAGDLPALEPNLTRDVTGGAHYPQDMQVQPMLAAAALLRAARDLG
                     ATVHTGTEVRGFLFGRDGAVTGVRAGSGDRAAGWVVNAAGTWGGEVADLAGAPLPVLPR
                     KGFVLVTEPLPRVIRHKVYTADYVAGVASDDAGLETSVVVEGTRAGTVLIGASRERVGF
                     DRRMSLPVIRELAAQAIGLFPFLADVTLLRTYLGFRPYCPDHLPVIGPDPRVPGLVHAC
                     GHEGAGIGLSAATGHLIAQTLTGATPDLDLAPFRPERFAA"
     misc_feature    complement(7703..7713)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            complement(7847..8671)
                     /locus_tag="FHR82_RS35620"
                     /old_locus_tag="FHR82_007252"
     CDS             complement(7847..8671)
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012738.5"
                     /locus_tag="FHR82_RS35620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007252"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_184814995.1"
                     /transl_table=11
                     /translation="MRRRGWFLGSAAVLVSVVVFVVPFAFIVLQAVKDPRGAAELDFSW
                     PDQFRFVENLVEVVTARDYMLVIAFINSTILTVGSVTVLVILAAMVAFVLQRRRTRWTG
                     LVNFLVLAGLIIPPAVVPTIWVLQGVGLFRTMAGLILVEVAFGLSFSILLFRNFLATVP
                     RELDESAVIDGAGPLRVFFQVIFPLLRSVAVTVIVVQSVVIFNDFVNPLYFLPGEQNAT
                     VQLTLYNFSSQYATQYNLLFMDILLITVPPLLMFLVANRRIVAGLTAGAVKG"
     gene            complement(8668..9525)
                     /locus_tag="FHR82_RS35625"
                     /old_locus_tag="FHR82_007253"
     CDS             complement(8668..9525)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012738.5"
                     /locus_tag="FHR82_RS35625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007253"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_184814996.1"
                     /transl_table=11
                     /translation="MTRSPYPHWFYLPAAVVYGTLFLVPTFASFYFALTRWTLFDQEFI
                     GVENFVTFFGEPALVQGLTNTLIYAVVTSGLKVVLGLPIAILLTSNIIARGFLRSAVFF
                     PVLVSTIGVGLTFTILMHPSNGLINDALAALGVQGPAWLTDPSYALLSVALVDVWKGVG
                     LATVIYIAGLVSIPREYLEAARVDGAGRWATFRHILLPLSMPATTTVIILSLIGGLRSF
                     DLIWAMTRGGPGFTSDVIASVIYKQYQAGFYGLSTAGNVVLFVLVTAIVLPLSRWLGRK
                     EVEL"
     gene            complement(9530..10843)
                     /locus_tag="FHR82_RS35630"
                     /old_locus_tag="FHR82_007254"
     CDS             complement(9530..10843)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013697.5"
                     /locus_tag="FHR82_RS35630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_007254"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_184814997.1"
                     /transl_table=11
                     /translation="MNPTRRTVLAAALGTALAITACSPGDLGSSDSEGRTTITFLVDNS
                     DASVRPAEGLAEAFMADHPDVVIEVEVRPQGGEGDNVVKTRLSTGDMADMFLYNSGSLL
                     QALKPRETLLPLNDEPFVDRLDETFTPTVSSGDRIFGAPFGAAVGGGVLYNRAVYDRIG
                     LAVPKTWTEFMANNAKIKAAGVAPVVQTYQDTWTSQLWVLADFHNVAAAEPDFADRYTA
                     NQTSYATSPAALQGFRHLQEIHDAGYLNADFAAQKYEDGLEELATGKGAHYPILTFVIG
                     TLETNNPGEVDDVGLFAVPGADAAENGLTVWLPGGVYVPTTTEGAELAAVKEFLDFVAS
                     PAGCDVQTKAYAPTGPYVVDGCELPADLPRAIQDMLPYFDTDGARTPALEFVSPVKGPA
                     LEQITVEVGSGIRSAEDGAALYDRDVAKQAKQLGLPGW"
ORIGIN
        1 cggcgccgct gagcaggacc agccgcacac cggcgtcggc gcagcgctgg acgacgcgct
       61 cggtgagccg cacgagccgg tccaggtcga taccggcgcg catcatgtcg ttgccgccgc
      121 cgttgaacga gagcagcgtc ggcggcggcg tcatggcgag cgccgtgtcc agctgggtgg
      181 tggcgatggg ttcgatcagc ctgccgcgga cggcgaggtt ggcgtagtgc acggtctcgc
      241 cggtcgcact cgcgagcccg gacgccacca ggtcggccca gccccgtgaa ctaccgtcgg
      301 gcaggtcgtc gccgacgccc tcggtgaagc tgtccccgat cgccacgtac cgcatgcccg
      361 cgtcactcac cgaccacgac cccggcctgg acgacggtcc ggcgcggccc ccacagcacg
      421 gcgggctcgg tgaacgggtc gccgtcgacg atcaccaggt cggcgacggc gccgacctgg
      481 acgcggccga ggtcgggcct gccgatgagg tcggcgttga cggacgtggc cgagcgcagc
      541 gcgttcagca ccccgtcgat ctcggactgc aggcgcaggc cgttgagctg ctcgtcgtcg
      601 aggtcgccca tcaggtcggt gccgaagccg atcgcgactc cggcggcgcg ggccagctcg
      661 atggcgttgg cgcccgcgtg gagcacctcg aggttcttct cctggctgac gggggcgagc
      721 ccgttgtccg cgccgcgccg ggagatcgcg tcgtaggtgg cgagggtggg cacgaggaag
      781 gcgccgcggg aggccatgag ggcggcggtc tcggcgtcca tgaggttgcc gtgctcgatc
      841 gagcggacgc cgttctcgac ggagtgccgg atcgcctcgg ccgagtaggc gtgggcggcg
      901 acgtagctgc cgcgcctggc cgcctcgtcg gtgacggcgc ggatctcctc ggccgagtac
      961 tgggggatcc ggatcgggtc ggcgagcgac atcaccccgc cggaggtcat catcttgatc
     1021 gcgtgggcac cgcgccggaa ccggtcgcgg gcggcggccc gcacggcgtc gacaccgtcg
     1081 acgacctcga ccatgtggac gtggcagccg cacacgtcac tgtcgactcc gcgtgcgtcc
     1141 ccgtgtccgc cggtctggct gagggcggcg ccggtgtaga ggtagcgggg tgcggggatc
     1201 gtgccctcgg cgatggcccg cgcgatgccg gggtctccac cggcgacgtc gcggacggtg
     1261 gtgaacccgc gcgccaacgc ggccttcagc cgccgggccc cggtgagcgc gaggtagctg
     1321 agcggggcgc cctcgatgga gagcaggtcg agcgagacgc cgtaggcgtg gcagtgcgcg
     1381 tcgatcagtc cgggtaggac ggtcccgcca cgggcgtcga gcacccggtc ggccgcgccg
     1441 gacccgccga tggccgagat gcggccgtct tcgacgagca ccggcccgtc gaccagctcg
     1501 gccgactccc cgtcgaacac cgccgcgttg atgatggcga gactcacgcg ctgctccctc
     1561 tcctccgtga ccttgagcac cgtacccagg cgaaaccgga accggtcggg tggctcgatc
     1621 gttcccccca aaacggacac gtgtggaaat ggggcacagt ggagcttgtc tacccgccgg
     1681 tgatcgccgc ctgcaagacc atgttccggg tcctggacct gaagctggac gtgcgtggcg
     1741 cagagcacat cccggcgtcc ggcggggccg tgctcgcctg cacgcacgtc agctacctgg
     1801 acttcatctt ctgtggcctg gccggcctgc cctcgggccg caagaccagg ttcatggcga
     1861 aggagcagat cttccgcaac cggatcgcgg gcccgctgat gcgtggcatg cgccacatct
     1921 cggtggaccg ggcggcgggc caggcctcgt accaggaggc ggtcgcgaag ctcagggcgg
     1981 gcgaggtcgt cggtgtcttc ccggaggcga cgatcagcag gtcgttcacg gtgaagccga
     2041 tcaagacggg cgcggtccgc atggccgagg aggcaggcgt cccggtcatc ccgatgacgg
     2101 tgtggggcag ccagcgcctg tggaccaagg gcaggccgaa gaacctcacg cagcggcacg
     2161 tccccgtcct gatccgggcg ggcgacccgt tccacccggg caaggacgac gacctggagg
     2221 cactgacagc cgacctccgc gagcggatga gcgccctgct ggaccaggca caggccgact
     2281 acccggagaa gccggccacg aaggagttct ggctcccggc gcacctgggc ggcagcgccc
     2341 cgacgccgga agaagcagcg gccctcgacg agcgccccag gggcagcaag cccacgacgt
     2401 aggggatcga cccgcgggcc atccggggcc ctgtccgtcc ggcgcgggca ggggccctcg
     2461 gctcactctg cccagatgcc ccgcacatgc cggatgtggc gatccatcaa ccgttccgcc
     2521 ccgtcgacgt caccagcctc gatgaggtcc agcagcgccg cgtgttcacg ggcggaaggc
     2581 accagctccc cctggtcggc cagcttccgc agcccgtaga tccgcgaccg ggaccgcagc
     2641 gaccgcaccg tctccaccag gtgggagttg ccgcccacgg cgagcaaccg aaggtggaac
     2701 tccatgtcgg cggcgacgtg agtgatgagg tcccccgccg cggcggcctc ctcgatggtc
     2761 gcggcgaggg gacgcaacga ctcgagcacc gacggggcga ccccggcagc ggtgatccgg
     2821 gcaacggtgg gcacctcgat caacgcccgc agctcactca gctcgtcgag gtccttcgac
     2881 gagaccgagg tgacccggaa ccccttgttc cgcaccgaat ccacgagtcc ctcactcgcc
     2941 aggtccagca tcgcctcccg gaccggggtg gcggacaccc cgaactgggc ggccaggctg
     3001 ggcgccgagt acaccacccc gggccgcaac tcgccggaga tcaccgcggc ccgcagggtc
     3061 tgcgtgatct cctcccgcag gttccgccga acacccagcg cggcaaagga atcagtcaca
     3121 actggaaccc ctggggaaat gggtcgtcgg gatcgaggaa gtactgcgcg gtaccggtga
     3181 tccacgcccg tccggtgatc tcgggcacca cggcgggcac gtcgccgacc gacgtcgaag
     3241 cgacgagccg cccggtgaaa cgcgtgccga tgaacgactc gttgcggaac tcggtgtcca
     3301 gtggcagctc accacgagcg tgcagctgcg ccatccgggc gctggtcccg gtcccgcagg
     3361 gtgaccggtc gaaccacccg ggatgaatgg ccatggcgtg ccgggaatgc cgggcgtcgg
     3421 agccgggcgc ggcgaggtag acgtggtggc acccggcgat cgaagggtcc accgggtgca
     3481 cgggtcgatc agaggagttg atggcgtcca tgatggacag tccggcggcg agcaaccgtg
     3541 gcccctcctc ccgggagaac ggcagcccga gctgctccac cccgaggatg gcgtagaagt
     3601 tcccgccgaa cgccatgtcg tagcgcaccg ggccgaaccc gggcacgggg acctcacggt
     3661 ccagccccac cgagaacgac ggcacgttgc ggatggtcac cgaccgcgcg accccgtcgg
     3721 acaccgccac gtcggccacg accaacccgg ccggggtgtc gagccggacg gtggtgaccg
     3781 gctcggcaac gggcaccatc ccggtctcca ccagcacggt cgcgactccg atggtgccgt
     3841 gcccgcacat cggcaggcac ccggacacct cgatgaacac cacgccccag tcggcgtcct
     3901 cgcgggcggg cggctgcagg atcgcgccgc tcatcgcgga gtgtccacgc ggctcgtaca
     3961 tcagcaactg ccggacgtgg tcgaggtgcg ccatgaagta ctcgcgccgc gcggacatcg
     4021 tgtccccggg aatcaccccg accccaccgg tgatcacccg ggtcggcatc ccctcggtgt
     4081 gcgagtcgac cgcgtggaag acacgtttcg cacgcatcag gacagtccct cggccagtgc
     4141 gcgctccgtc gcctgccgca ccgccgcctc gtgttcgggg gtcaacggca ccctcggctg
     4201 gcggcacggg cctccgtagc gccccacgat gtccatgctc agcttgatcg cctgcacgaa
     4261 ctcgacccgg gaatcccatc gcagcaacgg gtgcagcacc cggtagagcg gcagcgcggt
     4321 ggcgaggtcg ctctcggcgg ccgcctgcca gaactcgacg gtggacttcg gcagcgagtt
     4381 ggggaacccg gcgatccacc ccggcgaccc cgcgacggcc agttcgaggg cgacatcgtc
     4441 cgcgccgcac agcacgtcaa gtccgggtgc cagctcagcg atccggtagg gccgccgcac
     4501 gtccccggtg aactccttga ccccgacgat caacccttcc tggtacagct ccgcgagcaa
     4561 ctcgggcacg aggtcgatct tggtgtcgat cgggttgttg tacgcgacga cgggcacgcc
     4621 ggccttggcg acctcgcggt agtgcgcgag cacggcgcgc tcgtcgccac ggtaggcgtt
     4681 gggcggcaac gacatcaccg cgggacaccc ggcttcggcc gcctgctcgg cccaccgccg
     4741 cgactcggcc gaaccgtacg cgccggtgcc gggcatcacc gtgaacccgt cgggtgcggc
     4801 ctcgaccgcc gtgcgcacca ccctggcacg ctcctcgacg gtcagcgtcg ggtactcccc
     4861 cagcgacccg ttgggcgtga caccgtggca cccgttggcg gccagccacg ccacgtgctc
     4921 ggcgaaacgg tcgaagtcga cctcccctcg gtcggtcatc ggcagcgcgg tcgccaccag
     4981 aactccgtgc cagggcttgc tcatgcgtcc tccacacctt ccgcgagatt cccgagagtc
     5041 accggcgccg cgatgggccg cgccgccagc ccacgcagat cggccgcggt caccggccgc
     5101 ccacacgcct ccgcgaccag acacgcggtc gcgtacccgc acacccgccc ctggcagagc
     5161 cccatccccg gccgggcgag cagcttgaca ccgcgcggat cggtggcgcc gaggtccacg
     5221 acggactccc ggacctcccg caccgtgacc tcctcgcagc ggcacacgat cgtgtcgtcg
     5281 gccggccggc cggcgccgac ccggtaggtg tcgtgcatgg cggccgcgaa cgcccgcagc
     5341 gcggcccgct tccgcagcag cgaccgccgg gggcgcgccc cggcggcgtg tgccccggcg
     5401 atcgcaccct ccacgacgga cagttgagcg ccaccgaccc cgcacacctc accggccagg
     5461 tagacaccgg gcaccgaggc gcgctgccac tcgtcagccg tggcgacgag gctgccgtcg
     5521 gcgtcgagcc gggtgtcgca cccgagctgg agcggcagct ccagctgcgg ggtgaacccg
     5581 tacccgaccg cgaccgcgtc gcactcgatc tcccgctcgg acacgggccg ccggttccgg
     5641 tcgagccggg cgacggtgac agcgtgcacc cggtcgtcac cgtgcgcggc gaccactgcg
     5701 gtcctggtgt ggaacgggat ccggtgcccg tgcagtgccc tgaggtaccg ggcgccttcg
     5761 acgagcttgc cggggttgcg ggccacggcc agggggtggc gggcgaaccc gagaggggag
     5821 ccggcttcga aaacgccggt gacacggtgg cggagcgcgg cggcgacggg cagcaggaag
     5881 ggcccggtac cggcgacgac gacccggtcc ccgacgggca cgccgtggcc cttgagcatc
     5941 gcctgcgcgc cgcccgcggt gaacacgccg ggcagggtcc agccggggaa cgggagctgc
     6001 cggtcgtacg cgccggtcgc gacgatcacg gtgcgggcgc ggacctcacc ggcggtggtg
     6061 tggaggacga aaccgtctgc ttccacttcg acgtgccaga ccgcgtgacc ggccaggatg
     6121 tcctgccgca gggacgcggg gatcgggttg tgcccgtccc ggtgccgcca gtactgccct
     6181 cctggccgac cggcggagtc gaggagcgcg acccgcgcac cgcgaccgcg cgcggcggcg
     6241 gccgccgcga gcccggcagg accggccccg atcacggcga tgtcagcagg cgaggtggtc
     6301 acgaccggcc ccctcctggg acgacaccac gtcaccgtcg cggacctcgg tcaggcaggc
     6361 ccgcagaccg gggcgcccgt tgacgacgac caggcagtcg aagcagacac cgatgccgca
     6421 gaacaggccg cggggcgcac cgccgtgccg ggtggtccgc cacgagcggt acccggccgc
     6481 gagcagggcg gcaccgacac tctgcccggg ctccgccgtg acctcttcgc cgtcgaagga
     6541 gaacgtggtc atgccgcgaa cctctccggc cggaacggcg ccaggtcgag gtccggtgtg
     6601 gcgccggtca gggtctgagc gatgaggtgc ccggtcgcgg cggacaggcc gatgccggcg
     6661 ccttcgtggc cgcacgcgtg gaccaggccc ggcacgcgcg ggtcggggcc gatcaccggc
     6721 aggtggtccg ggcagtacgg ccggaacccg aggtaggtcc gcagcagtgt cacgtcggcg
     6781 aggaacggga acaacccgat ggcctgggcg gcgagttcgc ggatcaccgg cagcgacatg
     6841 cgccggtcga acccgacccg ttcccggctc gctccgatga ggacggtccc ggcgcgcgtg
     6901 ccctcgacca cgaccgaggt ctcgagcccg gcgtcgtcgc tggcgacgcc tgccacgtag
     6961 tcggcggtgt acaccttgtg ccggatgacc ctcggcagcg gctcggtgac cagcacgaac
     7021 cccttcctgg gcagtaccgg caacggcgcc ccggcgaggt cggcgacctc gccgccccag
     7081 gttccggcgg cgttgacgac ccatccggcg gcgcggtccc cggagccggc gcggacaccg
     7141 gtgaccgcgc cgtcccggcc gaacaggaac ccgcgcacct cggtgccggt gtggacggtc
     7201 gcgccgaggt cgcgtgcggc acgcagcagt gccgcagcgg cgagcatggg ctggacctgc
     7261 atgtcctgcg ggtagtgcgc gccgccggtc acgtcgcggg tgaggttggg ttccagggcg
     7321 ggcaggtcgc ccgcggtcac gtccacggcg tcgacgccgc acgcgcgctg ccgcccggtg
     7381 aggtccgcga gcccggccag cgccggggcc gacctggcga cgacgacgcc gcccttgtgc
     7441 tccagttcga gccggtccgc gccgatccgc tcccccagga ttcgccaccg ccgcacggac
     7501 agcagcgcga ggtccagttc gggcccgggt tccttgtcgg agaccaggat gttgccctcg
     7561 ccgccgctgg tggtgcccgt cgcgacgccg ccgcggtcca gcacggtgac ccgcagcccg
     7621 gcggcggcgc agtggtacgc gcaggcggcg ccgaccatcc cggcgccgat caccagcacg
     7681 tccaccgcca cctcagtagt atgtcacatg gcactgggtt agcgagagca acgaactatg
     7741 accaccggac cgcgtggtcg gttagcgtcc gtccgcgcgc acggtggtcg gcaccgtcac
     7801 ccctgcaggt ggcctcgtgc gaccgacggc gacggccacc gtgcgctcac cccttcaccg
     7861 cgcccgcggt cagcccggcg acgatgcggc ggttggccac caggaacatg agcagcggcg
     7921 gcacggtgat cagcaggatg tccatgaaca gcaggttgta ctgcgtggcg tactggctgg
     7981 agaagttgta gagggtcagc tgcacggtcg cgttctgctc gccgggcagg aagtacagcg
     8041 ggttgacgaa gtcgttgaag atcaccaccg actgcacgac gatcaccgtc accgccaccg
     8101 agcgcagcag cgggaagatc acctggaaga acacccgcag cgggccggcg ccgtcgatca
     8161 ccgcggactc gtcgagctcc cgcggcacgg tcgcgaggaa gttgcggaag agcaggatgg
     8221 agaaggacag cccgaacgcg acctcgacga ggatcagccc ggccatcgtg cggaacagcc
     8281 cgacgccctg cagcacccag atggtgggca ccacggcggg cgggatgatc agcccggcga
     8341 gcacgaggaa gttgaccagc ccggtccacc gggttctcct gcgctgcagc acgaacgcca
     8401 ccatcgcggc caggatcacg agcacggtca cgctgccgac ggtcaggatc gtgctgttga
     8461 tgaacgcgat gaccagcatg tagtcgcggg cggtgacgac ctcgacgagg ttttccacga
     8521 accggaactg gtcgggccac gagaagtcca gctcggcggc gccgcgcggg tccttcaccg
     8581 cctgcagcac gatgaacgcg aacggcacga cgaacacgac gaccgacacc aggacggccg
     8641 ccgacccgag gaaccagccg cgccgcctca cagctccacc tccttgcggc cgagccagcg
     8701 cgacagcggc agcacgatcg cggtcacgag cacgaacagg acgacgttgc ccgcggtcga
     8761 caggccgtag aacccggcct ggtactgctt gtagatcacc gatgcgatga cgtccgaggt
     8821 gaagcccggc ccgcctctgg tcatggccca gatgaggtcg aaggaccgca gcccgccgat
     8881 gagcgacagg atgatcaccg tggtggtggc cggcatggac aacggcagca ggatgtgccg
     8941 gaacgtcgcc caccggcccg caccgtccac tctggccgct tcgaggtact cgcgggggat
     9001 ggacacgagg ccggcgatgt agatgacggt ggcgagcccg acgcccttcc acacgtcgac
     9061 gagcgcgacg gacagcagcg cgtaggacgg gtcggtcagc cacgccgggc cctggacgcc
     9121 gagtgccgcg agcgcgtcgt tgatcagccc gttgctgggg tgcatgagga tcgtgaacgt
     9181 cagcccgacg ccgatggtgc tgaccagcac ggggaagaac accgcggagc gcaggaagcc
     9241 gcgggcgatg atgttcgagg tcagcaggat cgcgatgggc aggccgagca cgaccttcag
     9301 cccggaggtg acgacggcgt agatcagcgt gttggtgagc ccctgcacca gtgcgggctc
     9361 gccgaagaac gtcacgaagt tctcgacgcc gatgaactcc tggtcgaaca gggtccacct
     9421 ggtgagggcg aagtagaacg acgcgaacgt cggcaccagg aacagcgtcc cgtagacgac
     9481 cgcggcgggc aggtagaacc agtgcgggta gggcgagcgg gtcatgtgcc taccaacccg
     9541 gcagcccgag ttgcttggcc tgcttggcga cgtcgcggtc gtacagggcg gcgccgtcct
     9601 cggcggaccg gatgccggag ccgacctcga cggtgatctg ttcgagtgcc gggcccttca
     9661 ccggggacac gaactcgagc gcgggtgtgc gggcgccgtc ggtgtcgaag tagggcagca
     9721 tgtcctggat ggccctgggc aggtcggcgg gcagctcgca gccgtcgacg acgtacgggc
     9781 cggtcggcgc gtaggccttg gtctgcacgt cgcaccctgc cgggctggcg acgaagtcga
     9841 ggaactcctt gacggcggcc agttcggcgc cctcggtggt cgtcggcacg tacacgccgc
     9901 cgggcagcca cacggtcagc ccgttctcgg cggcgtcggc gccgggcacg gcgaacaggc
     9961 cgacgtcgtc gacctcgccg gggttgttcg tctcgagggt gccgatgacg aacgtgagga
    10021 tcgggtagtg cgcgcccttg ccggtggcca gttcctccag gccgtcctcg tacttctgcg
    10081 cggcgaagtc ggcgttgagg tacccggcgt cgtggatctc ctgcaggtgc cggaagccct
    10141 gcagcgcggc cggggacgtc gcgtagctgg tctggttggc cgtgtacctg tcggcgaagt
    10201 cgggttccgc ggcggcgacg ttgtggaagt cggcgagcac ccacagctgg gaggtccagg
    10261 tgtcctggta ggtctggacg accggggcga caccggcggc tttgatcttc gcgttgttgg
    10321 ccatgaactc ggtccacgtc ttcggcaccg ccaggccgat gcggtcgtag acggcgcggt
    10381 tgtagagcac gccaccaccg accgcggcgc cgaacggcgc gccgaagatc cggtcgccgg
    10441 aggacacggt gggggtgaac gtctcgtcga ggcggtcgac gaacggctcg tcgttgagcg
    10501 gcagcagcgt ttcccgcggt ttgagcgcct ggagcagtga gccggagttg tacaggaaca
    10561 tgtcggccat gtcccctgtg gacagccggg tcttgaccac gttgtcgccc tcgccgccct
    10621 gggggcggac ctcgacctcg atgacgacgt cggggtggtc ggccatgaac gcctcggcga
    10681 gcccttccgc cggccggacg ctcgcgtcgg agttgtcgac gaggaacgtg atcgtggtgc
    10741 ggccttcgct gtcgctggag ccgagatcac cggggctgca cgcggtgatg gcgagtgccg
    10801 tgccgagcgc cgcggcgagc accgttcgtc gggtggggtt catcgaaacc tccggggatc
    10861 agtggccggg ttcgaacaag ggggcgagga ggcgccgcag ttcggcggcg tgcggggcga
    10921 acccgccgtg ggtgatggcg tcgaccagct gggtggcggg ggcgtcgaga tagcgggcga
    10981 gccgggcggc ggccgcttct tcgcccggta cctcgccggt ggcggtggcg gcctcgacga
    11041 cccgggtcca ggtgggttcg tggtcgagga cgtcgcggac ggtggggtcg ccgggcaggc
    11101 ccggtccggt gcctggtccg gtgccgcacg ggtcggggac gtcccagtcg tggcggccgt
    11161 ggtggaccca caccggctgg tgcctgccgg ggacgtgcac ggtggcggtg gcgccgacgg
    11221 ggacggtgac gtgcagcagg aaccggccgt cggcgcgttg ccaggacacg gcggcctggc
    11281 cgtacggggt gtcgtggcgc gcggtggccc aggtcagcgc cggccgcggc cgagggcgga
    11341 ccagcagttc gcggtagccg gggctggcgg gggcgagccc ggcgacggcg cggtgcagcc
    11401 agtccgcgac cgcgccgagc gcgtagtggt tgaacgaggt catctcgccg gggttgacgg
    11461 tgccgtcggg cagcaggctg tcccagcgtt cccacacggt ggtggcgccc atggtgaccg
    11521 ggtagagcca ggacgggcag ccagtctgca gcagcagccg gtaggcgacg tcgagctcgc
    11581 ccgcctcggt gagcgcgtcc atgatcagcg gggtgccgac gaacccggtg gcggtgcgga
    11641 acccggcggt gcggacgagg tcggcgagcc gctcccccgc acgcgcgcgt tgctcgggtg
    11701 ccggcagcag tccccacacg agggcgagtg cgtacaccgt ggccgagtcg ctgaggagcc
    11761 gcccgccctc ggtggcgtac tcgcgggcga acgcctcccg cacctgcgcg gccaggtcgg
    11821 cgtaccggcg ggccacgggg tcgtcgccga gcaggtccgc ggtgtcggcg acgacctgtg
    11881 ccgagcgggc caggtgcgcg gtggcgacga catcggggtc cgcgcgcgcg gcgaacgggt
    11941 cctcgggcgg cgcggccggg tcgagccagt caccgaactg gaagccgccg gtccacagcc
    12001 ggtccttgcc cgcgaggccc gcgacgtggt cgacccacgc ccgcatgctc gcgaactggc
    12061 gggcgagcag ttcgcggtca ccggtgcgct ggtggacggt ccacggcacg agcacggccg
    12121 cgtcacccca ggcggccgcc gcgcggggcg ggccggcgag cacgtcgggc acgacgaacg
    12181 gcacggcgcc gttgtcgtgt tgttcgcagg cgaggtcggc gagccacgag gtgaggaaac
    12241 cggtgctgtc gaagaggaac tcggccgcag gcgcgaagac ctggatgtcg ccggtccagc
    12301 cgagccgctc gtcgcgctgc gggcagtcgg tcggcacgtc gaggaagttg cctcgggcgc
    12361 tccacacgac gttctcgtgg aaccggtcga gcagcgggtg ggagcaggag aaccagccgg
    12421 tgcggcgcag gtcggtgccg acgacgaccg cggtgacgtc ctcggcgcgc aggccgggca
    12481 cgccggtgac ctcggcgtac cggaagccgt ggaaggtcag ggcgggttcg agcacggcgt
    12541 caccgtcggc gaggacgtag ctgtcggtgg cgctggcggt ccgaagtgga cggacgcaca
    12601 gctcgccgtc cgccagcacc tccgcgtgcc ggacgacgac ctcggcaccg gccgcggcac
    12661 cgcgcacgcg cagccggacc cagccgacga g
//
