LOCUS       NZ_JACHJQ010000003     10399 bp    DNA     linear   CON 27-SEP-2024
DEFINITION  Actinophytocola algeriensis strain CECT 8960 Ga0436956_03, whole
            genome shotgun sequence.
ACCESSION   NZ_JACHJQ010000003
VERSION     NZ_JACHJQ010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinophytocola algeriensis
  ORGANISM  Actinophytocola algeriensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1136783)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1136783)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 154.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203735.1-RS_2024_09_27
            Annotation Date                   :: 09/27/2024 05:42:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,095
            CDSs (total)                      :: 9,030
            Genes (coding)                    :: 8,881
            CDSs (with protein)               :: 8,881
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2, 2 (5S, 16S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 44 of 149
            Pseudo Genes (incomplete)         :: 120 of 149
            Pseudo Genes (internal stop)      :: 3 of 149
            Pseudo Genes (multiple problems)  :: 17 of 149
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1053513
            Orig. end                         :: 1063912
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJQ010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    41..51
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            126..1694
                     /locus_tag="FHR82_RS17375"
                     /old_locus_tag="FHR82_003526"
     CDS             126..1694
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033086.1"
                     /locus_tag="FHR82_RS17375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003526"
                     /product="GAF domain-containing protein"
                     /protein_id="WP_225944042.1"
                     /transl_table=11
                     /translation="MFALQTRLTETAKSRDRLQGLLDAVVAVGAGLELEGTLNRIVRTA
                     VELVDARYGALGVVGPDQQELLSEFVYVGADEERGRGVVIENPHPIEEPVDAVPRHVLG
                     EPIRVRGEVFGNLYLTEKRGDDGEFTEDDEIVLKGLAAAAGVAVENARLFEESLLRERF
                     MAASREVNSHLLGGGSSQDALALIASRACELSAADCALLLLSDGARVVVRAAAGEVGPD
                     LVGTALPVNDALVASMFDMQGPEMIADLSGIMPATWRLPLGPAVAVPLRAGNVVTGVLL
                     AMRASGGARFPREHVPLLASFADQAAFALELAEKHRAQRQLDVLADRDRIAGDLHDHVI
                     QRLFATGMSLQGTVRRITDPTVRRRVSRAVEQLDETVREIRTSIFDLHTTGTDGSASLR
                     RRLLDIVEELSADSELTPSVRISGAVDTLVPEALAVHATAVLREAVSNAIRHSHAGSIV
                     VTVEAGADLIIDVVDDGVGMPPGIARSGLLGVERRAGKCGGMAVVVPGPEGGTRLTWRA
                     PLDGA"
     assembly_gap    2014..2112
                     /estimated_length=99
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2335..2979)
                     /locus_tag="FHR82_RS17380"
                     /old_locus_tag="FHR82_003527"
     CDS             complement(2335..2979)
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103002.1"
                     /locus_tag="FHR82_RS17380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003527"
                     /product="response regulator transcription factor"
                     /protein_id="WP_184811397.1"
                     /transl_table=11
                     /translation="MTIRIFLVDDHEIVRRGVADLFEDEPDLEIVGEAATVSDALRRIP
                     THQVDVAVLDVRMPDGNGVELCRDLRSQLPELHCLILTSYSDDEALLNAIMAGASGFVL
                     KQVVGNDLISAVRKVGAGESLLDAKTTEALMKRIRQGETDGPLAGLTDQERAVFDLVGE
                     GLTNRQIAARLFLAEKTVKNYVSHILAKLGMQRRTQAAVLAAELKSRKPDL"
     misc_feature    complement(2758..2760)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3065..3079)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.78"
                     /tool="antismash"
     gene            3202..3690
                     /gene="ectA"
                     /locus_tag="FHR82_RS17385"
                     /old_locus_tag="FHR82_003528"
     CDS             3202..3690
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156388.1"
                     /locus_tag="FHR82_RS17385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003528"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_184811398.1"
                     /transl_table=11
                     /translation="MSERVIDSPSKKDGAAIWRIARDSGKLDLNSSYAYLLWCHDFADT
                     SAVARVGDDVVGFVIGYLRPQAPDTVVVWQVAVDAGQRGRGTAAALLDALVRRTGARRM
                     ETTITTDNDASNALFRSLADRWGAEMSVTGEFAAHEFPDDHETEVLYRIGPFRDARAA"
     misc_feature    complement(3215..3235)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.14"
                     /tool="antismash"
     misc_feature    complement(3691..3707)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.08"
                     /tool="antismash"
     gene            3737..4987
                     /gene="ectB"
                     /locus_tag="FHR82_RS17390"
                     /old_locus_tag="FHR82_003529"
     CDS             3737..4987
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-356). E-value: 5e-73.
                     Score: 238.0. Matches aSDomain:
                     nrpspksdomains_FHR82_RS17390_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631928.1"
                     /locus_tag="FHR82_RS17390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003529"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_184811399.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-76, bitscore:
                     253.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFETVESEVRSYCRGWPVVFDRATGAWLHDEDGNAYLDFFAGA
                     GALNYGHNNPVLKKAVIDYLQRDGVVHALDMQTVAKREFLETFTDVIMKPRELDYKIMF
                     PGPAGANAVEAALKLARKVTGRESIINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDNYLDGQVEDFLWFERLLDDPGSGLNEPAAVIVETVQGEGGINAARDEWLRGLA
                     DLCKRHKILLIVDDVQMGCGRTGPFFSFESAGFEPDIVCLSKSIGAFGLPLAMTLLKPE
                     LDVWEPGEHNGTFRGISPAFIAASEAIRTYWTDDKLEKSTLAKGEQIGRVMNELAEQHP
                     GTPMRAKGRGLARGLAFDSGDIAGKVCRAAFERGLLMETSGPDGEVAKAMPPLTITDDE
                     LAKGLDLLSDAVHAVCS"
     aSDomain        3800..4804
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHR82_RS17390_Aminotran_3.1"
                     /evalue="5.00E-73"
                     /label="FHR82_RS17390_Aminotran_3.1"
                     /locus_tag="FHR82_RS17390"
                     /protein_end="356"
                     /protein_start="21"
                     /score="238.0"
                     /tool="antismash"
                     /translation="FDRATGAWLHDEDGNAYLDFFAGAGALNYGHNNPVLKKAVIDYLQ
                     RDGVVHALDMQTVAKREFLETFTDVIMKPRELDYKIMFPGPAGANAVEAALKLARKVTG
                     RESIINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDNYLDGQVEDFLWFER
                     LLDDPGSGLNEPAAVIVETVQGEGGINAARDEWLRGLADLCKRHKILLIVDDVQMGCGR
                     TGPFFSFESAGFEPDIVCLSKSIGAFGLPLAMTLLKPELDVWEPGEHNGTFRGISPAFI
                     AASEAIRTYWTDDKLEKSTLAKGEQIGRVMNELAEQHPGTPMRAKGRGLARGLA"
     gene            5001..5399
                     /locus_tag="FHR82_RS17395"
                     /old_locus_tag="FHR82_003530"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814642.1"
                     /locus_tag="FHR82_RS17395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003530"
                     /product="ectoine synthase"
                     /protein_id="WP_184811400.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.5e-54, bitscore:
                     176.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRNVGDLDDTDRDIKTPNWRSKRVVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFVVEGEGELLDKGTGETHQLRPGSLYLLNDHDHHQLRPRTDMRTV
                     CVFNPPVTGREVHDEHGVYPLIVEEKSA"
     gene            5396..6256
                     /locus_tag="FHR82_RS17400"
                     /old_locus_tag="FHR82_003531"
     CDS             5396..6256
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="FHR82_RS17400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003531"
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_184811401.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-20, bitscore: 70.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTATAVVDRYPTRIDGDPELIDRTDPSVWDFTGAGPIDRATMA
                     GHHTKGFSVHHDLLSPAEVAACRDELARLATDPGLAEDERAVLTDDGEVRMIYEVHAVS
                     ELVAELVRDPRVLDRARQILGSDVYIHQSRIDYLPGFRGKGTYWHSPFETWHAEDGMPA
                     PRAVSVSIPLSGQQELTGELMAMPGSHRTFVPTTLGSGGVPDKEHLRSLGAAYGIERFT
                     DAAGSVLVFDANTMQGSADNITPFPQTNIHVVFSSVENLLFAPFAGGQPRPPYLANRDC
                     EALRR"
     gene            6366..8486
                     /locus_tag="FHR82_RS17405"
                     /old_locus_tag="FHR82_003532"
     CDS             6366..8486
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.5"
                     /locus_tag="FHR82_RS17405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003532"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_184811402.1"
                     /transl_table=11
                     /translation="MAAEHPSAARFCLLGPVQLVVGERAVPLGGAGMRGVLAMLLLEPN
                     QVVALDRMVDVLWAHEPPPSARTMVQGYVSRLRQRIAEVDAGALIVTTPPGYRMVVDES
                     MIDVTVARSLLAKAREQPAARRAELLRAANALWRGPALADLGGRLTAPELTELRLAILE
                     ALYDAQLELGRHREVIGELTQLIDAQPFRERLVAQLMLALYRANRRATALDVYQRFAHR
                     AAQELGMDPGPQLRELHARVLRDDSGLLEPSPDPVITPKVGVLVPAQLPRAPSGFAGRE
                     DELDWLSGLLPPGETAAIGVLAGPAGVGKTALAVLWAHRVAQAFPDGQLFASMRGFDPQ
                     HAPVRAADVLTQFLLALGVGVDDVPFELDDRAALYRSILADRRVLVVLDDARDAEQIHP
                     LLPAASSSFVLVTSRIRLDGLVARTGAKLLSLGTLGVDAAERLIEHMAGTPAHSKDREQ
                     RRKLARLCGHLPLALRIAAARLAGNPQWTVADLVAELADERSRLGALDLADADTSVRAA
                     LDVTYRSLRPELAETVRHLGLFPGTRIGPHPVAALTGGDVDECRRRLRALAASFVVTET
                     SRDVFLMHDLVRLHAFALAEAEMSDVDRTLGQRRLLRYYLAGARTARALLRTDGVVRTE
                     TAGPPMATAEDAVDWIDQEWANLLAVVETGADSDNETDEEVVALARLAAEFSEGTRALG
                     PAALSRLVRLVV"
     gene            complement(8810..9604)
                     /locus_tag="FHR82_RS17410"
                     /old_locus_tag="FHR82_003533"
     CDS             complement(8810..9604)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238994.1"
                     /locus_tag="FHR82_RS17410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_003533"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_184811403.1"
                     /transl_table=11
                     /translation="MRTWNSWVAVGDSFTEGLSDEAPDGSYVGWADRLAATLAERNPGF
                     RYANLALRGKMLREIIEEQVPIAVEVKPDLVTICGGGNDLIVPGCDVDALAEEYEQAVK
                     ALRAAGCEVVMFTGPDPRDQPVVRRVRPKVAIYNANLRGIADRHGAMLVDLWAMKPLVD
                     VRAWSDDRLHFSPEGHRRIALRTAEVLDVPVTEEWREPWPPVEPTPWLRSRQADLAWTR
                     THLLPWVRRHLRGESMGDGLVPKRPELSPLALPARGKGGARV"
     misc_feature    complement(9700..9715)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.22"
                     /tool="antismash"
     misc_feature    10282..10297
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.47"
                     /tool="antismash"
     misc_feature    complement(10339..10352)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.63"
                     /tool="antismash"
ORIGIN
        1 accgatagcc cgtttgccga tccaacggac cagccgggga tgccatgcca cctgagcagg
       61 cgcgctcatc cgctcgtggg taccagcctg accgggtaca gccggacggg cggtcgctgg
      121 acggcttgtt cgcgctccag acgaggctga ccgagaccgc gaagtcgcgt gaccggctcc
      181 agggcctgct cgacgcggtg gtcgcggtcg gggccggact ggagctcgag ggcacgctga
      241 accgtatcgt gcgcaccgct gtggagctgg tcgacgcccg atacggtgcg ctcggtgtgg
      301 tcggcccgga ccagcaggag ctgttgtccg agttcgtcta cgtcggcgcc gacgaggagc
      361 gcggccgcgg ggtcgtcatc gagaacccgc accccatcga ggaaccggtc gacgcggtgc
      421 ctcgccacgt gctcggcgag ccgatccgcg tgcggggaga ggtgttcggc aacctctacc
      481 tgaccgagaa acgcggcgac gacggcgagt tcaccgagga cgacgagatc gtgctcaagg
      541 gcctggccgc ggcggctggc gtagcggtgg agaacgcgcg gctgttcgag gagtcactgc
      601 tgcgcgaacg cttcatggcg gcgtcgcggg aggtcaactc acacctgctc ggcggcggtt
      661 ccagccagga cgcgctggcg ctgatcgcgt cccgcgcctg cgaactgtcc gccgcggact
      721 gcgcgctgtt gttgttgtcc gacggtgccc gcgtggtggt ccgcgcggcg gcgggcgagg
      781 tcggcccgga tctcgtcggg actgccctgc cggtgaacga tgcgctggtc gcgagcatgt
      841 tcgacatgca gggcccggag atgatcgccg acctgtccgg gatcatgccc gcgacgtggc
      901 gcctgccgtt gggcccggcg gtcgccgtgc cgctgcgggc ggggaacgtg gtgaccgggg
      961 tgctgctggc gatgcgcgcg tccggcgggg cccggttccc gcgcgagcac gtgccgctgc
     1021 tggcgtcgtt cgccgaccag gccgccttcg cgctggagct ggcggagaag caccgcgcgc
     1081 agcgacagct cgatgtgctg gccgaccgcg accggatcgc gggcgacctg cacgaccacg
     1141 tgatccagcg cctgttcgcg acggggatga gtctgcaggg gaccgtccgc cggatcaccg
     1201 acccgaccgt gcggcggcgg gtcagccggg cggtggagca gctggacgag acggtccgcg
     1261 agatccggac gtccatcttc gacctgcaca ccacggggac ggacggttcg gccagcctcc
     1321 ggcggcggtt gctggacatc gtcgaggagc tgtcggcgga ctcggagctg accccgtcgg
     1381 tgcgcatttc gggggccgtc gacacgctgg tgccggaggc cctggcggtg cacgcgacgg
     1441 cggtgctccg cgaggcggtg agcaacgcca tccggcactc gcacgcgggg tccatcgtgg
     1501 tgacggtgga ggcgggggcg gatctcatca tcgatgtcgt cgacgacggg gtcgggatgc
     1561 cgccggggat cgcgcggagc gggttgctcg gggtggagcg gcgcgcgggg aagtgcgggg
     1621 ggatggccgt ggtggtgccc gggcctgagg gcgggacgcg gttgacgtgg cgcgcgccgc
     1681 tggatggggc gtgagggtgg ccgccggccc cctccaacca cgctaccgga aggcaccgac
     1741 atttccgtgt ggtcggtggc cggttgtcca caggtggttc cgttgtccac aggtgcgctc
     1801 tgagctgggc tttgttgtcg gtccccacac gtaggctgga gatcgggggc cggaggccaa
     1861 gccccgcccc gcgcggcttg acgcggcagg cgacggccac agacgctctc tcggcggctc
     1921 ggcggctcgg cggctcggcg gctcggcggc tcggcggctc ggcggctcgg cggctcggcg
     1981 gctcggcggc tcggcggctc ggcggctcgg cggnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nngtgggctc ccggtgcctg ccccggcctg gcctccccgt gcgagcgcgg
     2161 gcccggctcg aagggctcgt gctgcgcact cggcttcggc agcgtgggca gtcgcgcgtg
     2221 gcgaagccca cagtcgcggt caggccgccc cgtggggcgg cctccggccc ccggtcacca
     2281 ttgtcccagc cggtaccgac atgccgcgtc gcccggcgtc gagttgtcca cagttcacag
     2341 gtcgggcttg cgcgacttca gctcagccgc cagcaccgcc gcctgcgtgc gccgctgcat
     2401 gcccagcttc gccaggatgt gggacacgta gttcttgacc gtcttctccg ccaggaacag
     2461 cctcgccgcg atctggcggt tcgtcaggcc ctcgccgacc aggtcgaaca ccgcccgctc
     2521 ctggtcggtc aacccggcga gcggcccgtc cgtctcgccc tggcggatgc gcttcatcag
     2581 cgcctccgtg gtcttggcgt ccagaaggga ctcgccggcg cccaccttgc ggaccgcgct
     2641 gatcaggtcg ttgccgacca cctgcttcag cacgaaaccc gacgcgccgg ccatgatcgc
     2701 gttcaggagg gcctcatcgt ccgagtagga cgtcaggatc aggcagtgga gctccggtaa
     2761 ctgtgagcga aggtcccggc acagctcgac gccgttgccg tccggcatgc gcacgtcgag
     2821 taccgccacg tccacctggt gcgtcgggat gcggcgcagg gcgtcggaca ccgtggcggc
     2881 ctcgccgacg atctccaggt ccggctcgtc ctcgaagagg tcggcaacgc cccggcggac
     2941 gatctcgtgg tcgtcgacca agaagatcct gatggtcacg accggtcctc acaggcttcg
     3001 aacggccgct cgtggtcgcc ctgtgtccga tggttcgttc cgcaagctcc actcaccagg
     3061 cgctatatat cagataaatc gccatgatcg caacattacc gtggaccttc tcacagtaaa
     3121 aatcttgatt ccgcaggtga gcaacaagca ttgcattcgt tcatctgaag cggagaaaat
     3181 tctctgttac gttccttgag tatgtcggaa cgagtcatcg acagtccgtc caaaaaggac
     3241 ggagcggcaa tctggcgaat agcacgtgat tccggaaagc tcgatcttaa ctcgtcctat
     3301 gcgtatctgt tgtggtgtca cgacttcgcg gacacctcgg cggtggcacg ggtcggcgac
     3361 gacgtggtgg gcttcgtcat cggctacctg cgtccgcagg ccccggacac cgttgtggtc
     3421 tggcaggtcg cggtcgacgc cggtcagcgg ggcagaggca ccgcggccgc cctgctcgac
     3481 gcgctcgtgc gcagaaccgg cgcgcgtcgc atggagacca cgatcacaac cgacaacgac
     3541 gcgtcgaacg cgttgttccg ttcactcgcg gaccggtggg gtgccgagat gagcgtgacg
     3601 ggcgagttcg cggcgcacga gttcccggat gaccacgaaa ccgaagtcct ctatcggatc
     3661 gggccgtttc gggacgcgcg cgcagcctga ctgtaacgaa aagacacata aaccggggaa
     3721 gcaggttcag agcacagtga acgtattcga gaccgtcgag tccgaggtgc gcagctactg
     3781 ccgcggctgg ccggtcgtgt tcgaccgtgc caccggagcg tggctgcacg acgaggacgg
     3841 caacgcctac ctcgacttct tcgcaggcgc gggggcgctg aactacgggc acaacaaccc
     3901 ggtcctcaag aaggccgtca tcgactacct ccagcgcgac ggcgtcgtcc acgcgctgga
     3961 catgcagacc gtcgccaagc gggagttcct cgagaccttc accgacgtga tcatgaagcc
     4021 gagagagctc gactacaaga tcatgttccc cggcccggcc ggtgcgaacg ccgtcgaggc
     4081 cgcgctcaag ctggcccgca aggtgaccgg ccgcgagtcg atcatcaact tcaccaacgc
     4141 cttccacggc atgacgctcg gtgccctctc ggtcaccggc aactccatga agcgcggcgg
     4201 cgcgggcatc ccgctggtcc acgccacccc gatgccgtac gacaactacc tcgacggcca
     4261 ggtcgaggac ttcctgtggt tcgagcggct gctcgacgac ccgggttcgg gcctcaacga
     4321 gcccgccgcg gtgatcgtcg agaccgtgca gggcgagggc ggcatcaacg cggcccgcga
     4381 cgagtggctc cgcggtctcg ccgacctgtg caagcgccac aagatactgc tgatcgtcga
     4441 cgacgtgcag atgggctgcg gccgcaccgg cccgttcttc agcttcgagt cggccggctt
     4501 cgagccggac atcgtgtgcc tgtccaagtc gatcggcgcg ttcgggctgc cgctcgccat
     4561 gacgctcctc aagccggagc tggacgtgtg ggagccgggc gagcacaacg gcacgttccg
     4621 cggcatcagc cccgcgttca tcgccgcctc cgaggcgatc cgcacctact ggaccgacga
     4681 caagctcgag aagagcaccc tcgcgaaggg cgagcagatc ggccgcgtca tgaacgagct
     4741 ggccgagcag caccccggca cgccgatgcg ggccaagggc cgcggcctcg cgcgcggcct
     4801 ggcgttcgac tccggtgaca tcgcgggcaa ggtctgccgc gccgcgttcg agcgcggcct
     4861 gctgatggag acctccggcc cggacggcga ggtcgcgaag gccatgccgc cgctcacgat
     4921 caccgacgac gagctggcca agggactcga cctgctgtcc gacgccgtgc acgcggtgtg
     4981 ctcgtagggg aggaagaacc atgatcgtca ggaacgtcgg ggacctggac gacacagacc
     5041 gggacatcaa gacgccgaac tggagaagca aacgcgtcgt gctcgcgaag gagggcgtcg
     5101 gcttctccgt gcacgagacc acgctgtacg cgggcacggt caacgacttc tggtacgcca
     5161 accacatcga ggcggtgttc gtcgtcgagg gcgagggcga gctgctggac aagggcaccg
     5221 gtgagaccca ccagctgcgg cccggcagcc tctacctgct caacgaccac gaccaccacc
     5281 agctgcgccc caggacggac atgcgcacgg tctgcgtgtt caacccgccg gtgaccgggc
     5341 gcgaggtgca cgacgaacac ggtgtctacc ccctgatcgt ggaggagaag agcgcatgac
     5401 gctcaccgcc accgcggtcg tcgaccgata tcccacgagg atcgacggcg acccggagct
     5461 catcgaccgg accgacccca gcgtgtggga cttcaccggc gccggcccga tcgaccgcgc
     5521 caccatggcc gggcaccaca cgaaggggtt ctcggtccac cacgacctgc tgagcccggc
     5581 agaggtggcc gcgtgccgcg acgagctggc caggctcgcc accgacccgg ggctcgcgga
     5641 agacgagcgc gcggtcctca ccgacgacgg tgaggtccgg atgatctacg aggtgcacgc
     5701 ggtcagcgag ctggtcgccg agctggtccg ggacccgagg gtgctggacc gggcgcgcca
     5761 gatcctcggc tccgacgtgt acatccacca gtcccgcatc gactacctgc cgggcttccg
     5821 aggcaagggc acctactggc actcgccgtt cgagacctgg cacgccgagg acggcatgcc
     5881 tgcgccgagg gcggtcagcg tgtcgatccc gttgtcgggg cagcaggagc tgaccggtga
     5941 gctgatggcg atgccgggca gccaccgcac gttcgtcccc acgacgctcg gctccggcgg
     6001 ggtgccggac aaggagcacc tgcgctcgct cggggccgcg tacggcatcg agcggttcac
     6061 cgacgcggcg ggctcggtgc tggtgttcga cgcgaacacc atgcagggct cggccgacaa
     6121 catcaccccg ttcccgcaga ccaacatcca cgtcgtgttc tccagcgtgg agaacctgct
     6181 gttcgcgccg ttcgccggtg ggcagccgag accgccgtac ctggcgaacc gtgactgtga
     6241 ggcactgcgc cgctagtcca cccgggtgac gagcgcgacg gggaggtcgt gctcgtcacc
     6301 ccgggcgtcg taccttgtgc agtactcccg gcttcctgcg ccgggggaca actgggggtg
     6361 gtccgatggc agcggagcat ccgtccgccg cacggttctg cctgctcgga cccgtgcagc
     6421 tggtggtcgg ggagagggcc gtgccgctgg gcggcgccgg tatgcgtggc gtgctcgcca
     6481 tgctgctgct cgaacccaac caggtggtgg cgctggaccg gatggtggac gtcctgtggg
     6541 cccacgaacc gccgccgagc gcgcggacga tggtccaggg ctacgtgtcg cgcctgcggc
     6601 agcggatcgc ggaagtcgac gcgggcgccc tgatcgtcac cacgccgccg ggctaccgga
     6661 tggtcgtcga cgagtccatg atcgatgtga ccgtcgcgcg gtcgctgctc gccaaggccc
     6721 gcgagcaacc cgccgcgcgc cgcgccgagc tgctgcgcgc ggcgaacgcg ctgtggcgcg
     6781 ggcccgcgct cgccgacctc ggcggcaggc tcaccgcgcc ggagctcacc gagctgcgcc
     6841 tcgcgatcct cgaggcgctg tacgacgcgc agctggagct gggccgccac cgcgaggtga
     6901 tcggcgagct gacgcagctg atcgatgcgc agccgttccg cgagcggctg gttgcccagt
     6961 tgatgctggc cctgtaccgg gcgaaccgcc gcgccaccgc gctcgacgtg taccagcgct
     7021 tcgcgcacag agcagcccag gagctgggca tggaccccgg cccgcagctg cgtgagctgc
     7081 acgcacgggt gctgcgcgac gactccggcc tgctcgaacc ctcgcccgac ccggtgatca
     7141 caccgaaggt cggcgtgctg gtgccagcgc agctgccgag ggcgccgtcc ggtttcgccg
     7201 gtcgcgagga cgagctggac tggctgtccg ggctcctgcc gccgggtgag accgcggcga
     7261 tcggcgtgct ggccgggccg gctggcgtcg gcaagaccgc gctcgccgtg ctgtgggcgc
     7321 accgcgtcgc gcaggcgttc ccggacgggc agctgttcgc gagcatgcgc ggcttcgacc
     7381 cgcagcacgc gccggtgcgg gccgcggacg tgctcaccca gttcctgctc gcgctcggcg
     7441 tcggggtgga cgacgtgccg ttcgaactgg acgaccgggc cgcgctgtac cggtcgatcc
     7501 tcgcggaccg gcgcgtgctg gtggtgctcg acgacgcccg cgacgccgag cagatccacc
     7561 cgctgctgcc tgccgcgtcg tcgtcgttcg tgctggtgac gagccggatc cggctcgacg
     7621 ggctggtcgc gcggaccggc gcgaagctgc tgtcgctcgg cacgctcggg gtcgacgctg
     7681 ccgagcggct gatcgagcac atggcgggca cgcccgcgca cagcaaggac cgcgagcagc
     7741 gccgcaagct cgcgcggctg tgcgggcacc tgccgctcgc gctgcgcatc gcggccgccc
     7801 ggctcgccgg taatccacag tggacagtcg cggacctggt ggccgagctg gccgacgagc
     7861 ggtcccggct cggcgcgctc gacctggcgg acgcggacac gagcgtgcgt gccgcactcg
     7921 acgtcaccta ccgcagtctg cggccggagc tggccgagac cgtccgccac ctcgggctgt
     7981 tccccggcac gcggatcggc ccgcacccgg tcgccgcgct gaccggtggc gacgtcgacg
     8041 agtgcaggcg caggctccgc gcgctcgccg cctcgttcgt cgtcaccgag accagccggg
     8101 acgttttcct catgcacgac ctggtgcgtt tgcacgcgtt cgcactggcg gaagccgaga
     8161 tgtccgatgt ggacagaacg ctcggacagc ggcggctgct gcggtactac ctggccggcg
     8221 cgcggaccgc ccgcgcactg ctgcggaccg acggcgtggt gcgcacggaa accgcgggcc
     8281 cgccgatggc cacggctgag gacgccgtcg actggatcga ccaggagtgg gcgaacctgc
     8341 tcgcggtggt cgagaccggt gccgactccg ataacgaaac ggacgaggag gtcgtcgcgc
     8401 tcgcgcggct cgcggcggag ttttccgagg gtacgcgggc tctcgggccc gcggcgctga
     8461 gccggttggt ccgtctggtc gtctaggttc cacccgcgtt tagcgaacag ttagtcaatt
     8521 cgggtgatca agtggcaggc tgcccgccga gtgctcgacg tcccggagcc tgacatgagc
     8581 actcgggcgg cactaccaac gtgggggtgt gctcgcccaa gcgatccggc cggcgctgtt
     8641 gggggacagt gccggccgga ctgttccgcc ttcccggtat ttaaaagtgt tggtcccgac
     8701 ccggcttctc ctgcggggcc gcacgtggtt ggtggctggg tgccgtcacg ttcccctgag
     8761 gtaactgctg atcagcccca gactttcgcg caaggatcaa gggcgcgccc tagacgcggg
     8821 cgccgccctt gccgcgagcg ggcagggcga gcggcgacag ttccgggcgc ttgggcacga
     8881 gcccgtctcc catggactca ccgcgcaggt gcctgcgcac ccacggcagc aggtgcgtgc
     8941 gggtccacgc gaggtcggcc tggcgggacc gcagccacgg cgtcggttcg accggcggcc
     9001 acggctcccg ccactcctcg gtcaccggca cgtcgagcac ctcggcggta cgcagcgcga
     9061 tgcggcggtg gccctccggg gagaagtgca gccggtcgtc gctccaggcg cggacgtcca
     9121 cgaggggctt catggcccac aggtcgacga gcatggcgcc gtgccggtcg gcgatgcccc
     9181 gcaggttcgc gttgtagatc gcgaccttgg gccgcacccg ccgcaccacc ggctggtcgc
     9241 gcgggtcggg accggtgaac atgacgacct cgcagccggc ggcacgcaag gccttcacgg
     9301 cctgctcgta ctcctccgcc agcgcgtcga cgtcgcagcc gggcacgatc aggtcgttgc
     9361 cgcctccgca gatcgtcacc aggtcgggct tcacctcgac cgcgatcggc acctgctcct
     9421 cgatgatctc cctcagcatc ttcccccgaa gtgccaggtt cgcgtagcgg aaacccgggt
     9481 tgcgctcggc gagcgtcgcg gccaggcggt cggcccaccc cacgtagctg ccgtccggtg
     9541 cctcgtcact caaaccttcg gtaaagctgt ccccaacggc aacccagctg ttccatgtcc
     9601 gcacggcgac cacccctccc tagtgcgcgc ccccgtttat atctcctaat ccgccaggat
     9661 gccgcctaat tcccgacttc cgccaccgtc ggtttgccgt gttcgccacc ggctgcacag
     9721 cgtgatcagg gggttgccag ttcgatgccg ccgcgtacca gcgcgagcgc ctcgaccgcg
     9781 gccgccgggg cgagcgcttt gtccgggtcg gacagcatca cggtggcgac gaccgtgcgg
     9841 ccgtcgtcgg tgcgtgccag gtgattcaac accagaagac ccggttcgga cccgcctttg
     9901 aaccagatct ccgggtactc cgttcggtcc agcccgatac cggcgtcctc aatcgacatc
     9961 gcctcgtcga tgcgcgggct gtgtgccgcg tcgaggctcg ccagcacgcg gcacatgtcg
    10021 ttcgcggagc cgaaccactc gatcgaatcg aggttcctcg gttcggtcca cggctggatg
    10081 gcggtgcggg gcacggccgc gagcgcaggc cgcagcgcgg gacgcaacgg gcgggggagc
    10141 gacaggtagg tgttcgcgag cttcgggtac cggaaaccct tgaggacgaa cagttcccgc
    10201 gtggtgagga gcgcgtcgtt gcgcgggtcg cggttgccgg tcgccgccat ctgcctgcgc
    10261 accgcgtcgc ggccgacgtg gtggaccagg tggtcggtgg ccgtgttgtc gctaatcgag
    10321 atcatcaggt cggcgtgctg ccgcagcgtg acgtccgtgc ccgcgggacg gtcctgcagc
    10381 acgccggtcg ggaggctct
//
