LOCUS       NZ_JACHJQ010000002     10834 bp    DNA     linear   CON 27-SEP-2024
DEFINITION  Actinophytocola algeriensis strain CECT 8960 Ga0436956_02, whole
            genome shotgun sequence.
ACCESSION   NZ_JACHJQ010000002
VERSION     NZ_JACHJQ010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinophytocola algeriensis
  ORGANISM  Actinophytocola algeriensis
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1  (bases 1 to 1323779)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1323779)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 154.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203735.1-RS_2024_09_27
            Annotation Date                   :: 09/27/2024 05:42:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,095
            CDSs (total)                      :: 9,030
            Genes (coding)                    :: 8,881
            CDSs (with protein)               :: 8,881
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2, 2 (5S, 16S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 44 of 149
            Pseudo Genes (incomplete)         :: 120 of 149
            Pseudo Genes (internal stop)      :: 3 of 149
            Pseudo Genes (multiple problems)  :: 17 of 149
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 122548
            Orig. end                         :: 133382
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJQ010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10834
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10834
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10834
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5834](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5834)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(104..118)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    310..321
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.21"
                     /tool="antismash"
     gene            1061..2575
                     /locus_tag="FHR82_RS06790"
                     /old_locus_tag="FHR82_001377"
     CDS             1061..2575
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672806.1"
                     /locus_tag="FHR82_RS06790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001377"
                     /product="NCS2 family permease"
                     /protein_id="WP_184809442.1"
                     /transl_table=11
                     /translation="MVTDRIPRVPSDTSPGRRAPGAIDKYFGISERESTFGREVRGGFA
                     TFFAMSYIVVLNPLIIGTVADADGNLLGGAGSVPESIAMVGSVTSLAAGLLTILMGIVG
                     RYPFAIAVGLGLNSFLAVSVASQMSWQEAMGLVLIEGVVITLLVITKVRIAVLHAIPSD
                     LKTAMSVGIGLFIAFIGLVDSGFIRRIPDEAGTTVPVNLGVGGTLAGWPIVVFLVGLIL
                     TAVLVMRKVRGAIIIGMATSTVLAIIVEALFKPGPSLVDGKPVPTGWQLNVPTLPSDIV
                     AVPDLSLIGDVDPFGGFARVGVLAALMIVFTLMLSDFFDTMGTVVGLSAEAKLLDKDGQ
                     VPGVGRVLLVDSLAAASGGAASTSSNTTYIESAAGIGEGARTGFANIITGGLFLVAMFI
                     APLVSIVPFEAATPALVIVGLAMMAQIRRIDFSDVAIVIPCLLTAVLMPFSFSIAVGLG
                     AGMITYVILRTFQGRAREVHPLLWGVAALFALYFAAHPIRVALGID"
     gene            2597..3118
                     /locus_tag="FHR82_RS06795"
                     /old_locus_tag="FHR82_001378"
     CDS             2597..3118
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017170.5"
                     /locus_tag="FHR82_RS06795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001378"
                     /product="DUF742 domain-containing protein"
                     /protein_id="WP_184809443.1"
                     /transl_table=11
                     /translation="MDLFGMYGSRSDDEPANTPEPSPGEPVAEPPPVEPEEDTAPVERI
                     PSARDDAVRHFIDSMEGVEFDGQIVRPYARTGGRTRSVAGLELETLLSLTVADVPGNLP
                     PDHYAVAELCRRPVSVAEVAGYRSLPIGAARVIISDLIETGFLATSGPETAGAPTVDLM
                     RRVLEGLHRL"
     gene            3141..3950
                     /locus_tag="FHR82_RS06800"
                     /old_locus_tag="FHR82_001379"
     CDS             3141..3950
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FHR82_RS06800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FHR82_001379"
                     /product="hypothetical protein"
                     /protein_id="WP_184809444.1"
                     /transl_table=11
                     /translation="MRPRLLLVTDLGYAAAGRTYRDEDIDLAGRLRADFDVAMCHPLDA
                     AAVMRGFDLVLVRNTGPVLHYAEQYAAFRRQALDAGTRVFTQLTGKADMAGKQYLLDLT
                     SAGYPVIPTVDRAEDVGRLPAADEYVVKPKFGADSVGLRFVSRSELGAVTDQLVQPRIR
                     FRYEVSFYFVDRDFQYALHAPDPGSRWGLVPYAATAADLAFAQRFVDWNDVDHGIQRVD
                     ACRTEDGDLLLVELEDLNPFLSLDRVPPATRDAFVAALTASLLRMAR"
     gene            4010..4618
                     /locus_tag="FHR82_RS06805"
                     /old_locus_tag="FHR82_001380"
     CDS             4010..4618
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810338.1"
                     /locus_tag="FHR82_RS06805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001380"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_184809445.1"
                     /transl_table=11
                     /translation="MVEQNLWLQKVADNPGHSSWYVERFRSMARAGDDLAGEARLVDAM
                     VPRRARILDAGCGPGRVGGALAEAGHEVVGVDVDPVLIEAAEQDHPGPRWLVGDLAELD
                     LPASGVTEPFDALVCAGNVMTFLAPGTRREVLTRFRTHLADDGRAVIGFGAGRGYPFDE
                     FLADAKASGLVPGLLLSTWDLQPYAEDADFLVAILRPAS"
     gene            complement(4675..5004)
                     /locus_tag="FHR82_RS06810"
                     /old_locus_tag="FHR82_001381"
     CDS             complement(4675..5004)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FHR82_RS06810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FHR82_001381"
                     /product="hypothetical protein"
                     /protein_id="WP_184809446.1"
                     /transl_table=11
                     /translation="MTARDVLAARQLALVRALLAGGPVPDGFDANRVGVEAAALRSKRR
                     SIASHLRPDLADLLEDRFGPLFDTWARDHPKPVELSFRADLDRFETWLYDEGHLERPRR
                     RWFRR"
     gene            complement(5001..5834)
                     /locus_tag="FHR82_RS06815"
                     /old_locus_tag="FHR82_001382"
     CDS             complement(5001..5834)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671915.1"
                     /locus_tag="FHR82_RS06815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001382"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_225943818.1"
                     /sec_met_domain="DUF692 (E-value: 2.7e-86, bitscore: 285.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRRLGMTGAPRLGVGIGWRPEIDLTVEALPGVDFVEVLAENIHA
                     GPLPESLTVLRARGVPVVAHAVSLSLGGAEPVDAARVQHLAAVAAAVGAPLVSEHVAFT
                     RAGGLDSGHLLPVPRTRDALNVLVDNVRRAQADLPVPLALENIAAVFDWPDNELTEGQF
                     LAELVDRTGCLLLVDVANLYANAVNIGTAPAVFLDELPLERLAYVHVAGGVTHDGVYHD
                     THAHAVPSEVLAVLAELCARVDPPGVLLERDDDYPADADLAAELAAIRAVAVRGR"
     gene            complement(5813..6748)
                     /locus_tag="FHR82_RS06820"
                     /old_locus_tag="FHR82_001383"
     CDS             complement(5813..6748)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="FHR82_RS06820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001383"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_184809447.1"
                     /transl_table=11
                     /translation="MHPWGLSGPEFLWLYTAGLVAGLVVAIGARMKVRRPRLAEPPGHL
                     DVTELGFLGGGPVNAVQVAVARLAEAGLVRVNRSGQVSATSGALSTGHPLDDAVLAQVT
                     KPRMVAAIVRRAPVEKQVTAIGESLVRRGLLVAPSAAIRARRLGPLLLYVVFVAGIVRW
                     INGAGNYLPVGYLTFLLAATLIAVFFLGSRNLTKPKARTVHGDRVVAEVRRRGSGAGQL
                     ERAAVSGPRVIEDKDLSHALALAVPVALIPVAWSYATSGSFSTHVPATSSSGSSGGGGG
                     HSCGSSSSSSCGSGGSSCGGGGGGCGGGSG"
     gene            complement(6922..8109)
                     /locus_tag="FHR82_RS06825"
                     /old_locus_tag="FHR82_001384"
     CDS             complement(6922..8109)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945963.1"
                     /locus_tag="FHR82_RS06825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001384"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_184809448.1"
                     /transl_table=11
                     /translation="MNLDGVFRREYGRAVSVLVRSLGDIGLAEEVVQDAFVEAVRRWPA
                     DGVPPSPAGWLITTARNRAIDRLRREATREERHRQALELLAAERGDEEGEEGVVRDDRL
                     RLIFTCCHPSLAMGVRVALTLRLLGGLTTEEVARAFLVPEPTMAQRLVRAKNKIRDAGI
                     PYRVPYEADLPARLGGVLAVLYLIFNEGYSTVDRPELSAEAIRLGRVLAALMPDEPEVT
                     GLLALMLLSESRRPARVTDDGELVLLPDQDRGRWDRGMVEEGHALVRQCLRRGRPGPYQ
                     IQAAINAVHSDAPTTGETDWAQVVALYDQLMVVAPTPVVALNRAVAVAELRGPAEALDL
                     VDALALDRYHLHHSVRGNLLRRLGRPDEAAAAYETALRMATTDADRTVVRRLLDG"
     gene            complement(8106..8438)
                     /locus_tag="FHR82_RS06830"
                     /old_locus_tag="FHR82_001385"
     CDS             complement(8106..8438)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438428.1"
                     /locus_tag="FHR82_RS06830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001385"
                     /product="YnfA family protein"
                     /protein_id="WP_184809449.1"
                     /transl_table=11
                     /translation="MTVAKSLVLFVLAALAEIGGAWLVWQGWREDRGLWWIAAGVVALG
                     AYGFVATFQPDPHFGRILAAYGGVFVAGSLAWGVVVDKFQPDRWDYVGAALCLAGVAVI
                     MYAPRR"
     gene            complement(8524..8862)
                     /locus_tag="FHR82_RS06835"
                     /old_locus_tag="FHR82_001386"
     CDS             complement(8524..8862)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659694.1"
                     /locus_tag="FHR82_RS06835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001386"
                     /product="YciI family protein"
                     /protein_id="WP_184809450.1"
                     /transl_table=11
                     /translation="MKQYLLSIYQPDGTPEPEALEPVMRNLDALNKEMRAAGVWVFAAG
                     LHPPETATVLRATGEEVLVTDGPFIEAKEFLGGFTVIRAEDLDAAMEWGRKLAAVLSPL
                     AIEVRPFQ"
     gene            8954..9505
                     /locus_tag="FHR82_RS06840"
                     /old_locus_tag="FHR82_001387"
     CDS             8954..9505
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421230.1"
                     /locus_tag="FHR82_RS06840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001387"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_225943817.1"
                     /transl_table=11
                     /translation="MISYGGLVYLITGATGTVGRPLLAELDGHAVRPVTRDPSRLPGAV
                     AEPDVTGVTAVFLHPRAVGLGAADLLVRAKAAGVRRVVVLSAVNVDDPLDEQPSRANGD
                     RDTEVEAAAIGSGLEWVSAVAAAGMVEHGHRPEFVAALMARYERENGRPAHVSGDVENV
                     LGRPARSFAEWVADHAEYVR"
     gene            9502..9843
                     /locus_tag="FHR82_RS06845"
                     /old_locus_tag="FHR82_001388"
     CDS             9502..9843
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024953.5"
                     /locus_tag="FHR82_RS06845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001388"
                     /product="DoxX family protein"
                     /protein_id="WP_184809451.1"
                     /transl_table=11
                     /translation="MTVVHIGLVLVAIAANAFSAVADFLRLERIRGGMTRVGVPTRILP
                     WLGVPKALAVAGLLAGLAVPWLAVAAAAGLVLFFLLAIGTHLRAGDRMIGLPLGFLALA
                     VAVVATAPV"
     gene            complement(9827..10729)
                     /locus_tag="FHR82_RS06850"
                     /old_locus_tag="FHR82_001389"
     CDS             complement(9827..10729)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628639.1"
                     /locus_tag="FHR82_RS06850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHR82_001389"
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="WP_184809452.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 5.9e-46, bitscore:
                     152.2, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRPVWQACPLLPSLEERLRTNHKIVDEPSDDVVAAITSSRFGVT
                     NEQIDALPSLRAIVHFGVGYETTDVPYARSRGIVVSNTPDVLTDCVADLAVGGMIDVLR
                     RLTAADRFVRRGDWRRGQFPLATKVTGRRVGILGLGRIGRAIAHRLAAFDARLSYHSRH
                     QVPGLPYTYHDSPAALAEAVEVLVVATSGGPATAGLVSSDVLAALGPAGYLVNIARGSV
                     VDEPALVAALTTGGIAGAALDVFADEPNVPEPLLGLDNVVLLPHIASATVETREAMGDL
                     AFRNLHQFLTDGTLLTPVP"
ORIGIN
        1 ctgaccgggc ggacggagtc gatcacgctg tcggtgctgg cgcggtccgc cgcggacgag
       61 gcggcgttcc tgatgccggt gccgtccagg gcacggttcg aggtggccga cgcggacctg
      121 ttcgccgagc tcgacgagat cagcaagccg gacatcgagg tgcgggaggt cgaggtcgac
      181 ggcgacggat cggggggtgc ggcgccgggt gggagcggcg ccaccgtcgt cgaccacgtc
      241 gaggtcggcc cgttcgacgt cgcccagctc gcgggcaccg acgccaccgc ggtgacggac
      301 tggttggggg acaacggttt cacgatgccc caggacctgt ccggggcgct caccccgtac
      361 ctcgcggaag gctggctcgt cgtcgcggtg cggctggcgc ccgcgtccgg gaacctcgag
      421 gaggggctgc cgccgatgcg ggtgtcgttc gagaccgaca ccccggtcta cccgatgcgc
      481 ctgtccgcga ccgccgagga cgtccagccg ctgcgcctgt acgtcctcgc cgaccaccgc
      541 gtcgacatca gcaacccggc cccggcgggc gccgcgccgg agctgacgtt cgcgggccgg
      601 gtcgagccgg gccccgagca cccgacactc tcggaaatgt tgccagggcc gcgtttcctc
      661 acccgctacg acggtgagtt cgcaccggac ctgatcgaga gcgatgtccg gatcaccacc
      721 gccaccaccg atgagccgta ccgcgcggtc gtggtggtga ccgagtacgt cagatcgtcg
      781 tggcgcgagg tggtactggc catcatcgtg ctgctcgcgg tggtgggcgt cgcgatcgtc
      841 gcactccggc gtcgacggtc ccgagtagac ggacgcgcct agtctacagt ggactgcgcg
      901 aatgggaacg cgcggtgaat cgtttcaccg gacaggtcgc gacgcaccgc gtgcagatac
      961 ctccgaaagg cctattgacc ggcggtgaca tgtcgttacc gtgggcgcgg cacaccgcca
     1021 catgatcgcg catcgcgcct gccagaagga caaccgaaac atggtcacgg atcgcattcc
     1081 ccgggttccg agcgacacgt cccccggtcg cagagccccc ggcgcgatcg acaagtactt
     1141 cggtatttcc gagcgcgaat cgacattcgg ccgcgaggtc cgcggtggtt tcgccacgtt
     1201 cttcgcgatg tcctacatcg tcgtgctcaa cccgttgatc atcggcacgg tcgccgacgc
     1261 ggacgggaac ctgctcggcg gcgcgggcag cgtgccggag agcatcgcga tggtcggcag
     1321 tgtcacctcg ctggcggccg ggctgctgac gatcctcatg ggcatcgtcg gcaggtaccc
     1381 gttcgcgatc gcggtcggtc ttggcctcaa ctcgttcctc gcggtgtcgg tggcgtcgca
     1441 gatgtcgtgg caggaggcga tgggcctggt gctcatcgag ggcgtggtca tcacgctgct
     1501 cgtgatcacg aaggtccgga tcgcggtact gcacgccata ccgtcggacc tcaagacggc
     1561 gatgagcgtc ggcatcgggt tgttcatcgc gttcatcggg ctcgtggact ccgggttcat
     1621 ccgccgcatc cccgacgagg cgggcaccac cgtgccggtg aacctgggcg tcggcggcac
     1681 gctggccggc tggcccatcg tcgtgttcct ggtcggcctg atcctgaccg cggtcctcgt
     1741 gatgcggaag gtgcgcggcg cgatcatcat cggcatggcg acgagcacgg tcctcgccat
     1801 catcgtggag gcactgttca agccgggccc gtcgctggtg gacggcaagc cggtgccgac
     1861 cggctggcag ctgaacgtgc ccacgctgcc gtccgacatc gtggcggtgc cggacctgtc
     1921 gctcatcggt gacgtcgacc cgttcggcgg cttcgcccgc gtcggtgtgc tcgccgcgct
     1981 gatgatcgtg ttcaccctca tgctgtcgga cttcttcgac accatgggca ccgtggtcgg
     2041 cctgtccgcc gaggcgaagc tgctcgacaa ggacggccag gtgccgggcg tcggccgcgt
     2101 cctgctggtc gactccctcg cggcggcgag cggtggcgcg gcgtccacgt cgtcgaacac
     2161 gacctacatc gagtcggccg ccggcatcgg ggaaggcgcg cgaacaggct tcgcgaacat
     2221 catcaccggc ggcctgttcc tggtggccat gttcatcgcg ccactggtgt cgatcgtgcc
     2281 gttcgaggcg gcgaccccgg cactggtgat cgtcggcctg gcgatgatgg cgcagatccg
     2341 ccgcatcgac ttctccgacg tcgccatcgt catcccgtgc ctcctcacgg ccgtcctgat
     2401 gccgttctcg ttctcgatcg cggtcggcct cggcgccggg atgatcacgt acgtgatcct
     2461 gcggaccttc cagggccggg cacgggaggt gcacccgcta ctgtgggggg tggccgccct
     2521 gttcgcgctg tacttcgcag cacacccgat ccgcgtggca ctgggcatcg actgacgacc
     2581 cgcacaggcg gacggcatgg acctcttcgg gatgtacggt tcgcgctccg acgacgaacc
     2641 agccaacaca ccggaacctt cgcccgggga gccggtggcg gagccgccgc ccgtcgagcc
     2701 ggaggaggac acggccccgg tcgaacgcat cccgtccgcg cgcgacgacg cggtccgcca
     2761 cttcatcgac tcgatggagg gcgtcgagtt cgacggtcag atcgtccgcc cctacgcccg
     2821 caccggcggc cgaaccaggt cggtcgccgg tctcgagctg gagaccctgc tgtccctgac
     2881 cgtcgcggac gtgcccggca acctgccccc ggaccactac gcggtcgccg aactgtgccg
     2941 caggccggtg tcggtcgccg aggtggccgg ctaccggtcg ctgccgatcg gcgcggcccg
     3001 cgtcatcatc tcggacctga tcgagacggg cttcctcgcc acgtcgggcc cggagacggc
     3061 gggcgcgccg accgtcgacc tgatgcgccg cgtcctggaa ggcctccacc gcctctgaaa
     3121 ccactggtta gagtgcgcgg gtgagaccac gtctgctgct cgtcaccgat ctcgggtatg
     3181 ccgccgccgg gcggacgtac cgggacgagg acatcgacct cgcgggccgg ttgcgggcgg
     3241 acttcgacgt cgccatgtgc caccccctgg acgcggccgc cgtgatgcgc gggttcgacc
     3301 tcgtgctcgt gcgcaacacc ggcccggtgc tgcactacgc cgagcagtac gcggcgttcc
     3361 ggcggcaggc gctcgacgcg ggcacccggg tgttcacgca gctcaccggc aaggccgaca
     3421 tggccggcaa gcagtacctg ctggacctca cctcggccgg gtacccggtg atcccgacgg
     3481 tcgaccgggc cgaagacgtc ggcaggctgc ccgccgcgga cgagtacgtg gtgaagccga
     3541 agttcggggc cgactcggtc gggctgcggt tcgtgtcccg ttccgagctg ggcgcggtca
     3601 ccgaccagct ggtccagccg cggatccgct tccgctacga ggtctcgttc tacttcgtcg
     3661 accgcgactt ccagtacgcc ctgcacgcgc cggaccccgg cagcaggtgg ggcctcgtgc
     3721 cctacgcggc gaccgccgcc gacctcgcgt tcgcgcagcg gttcgtcgac tggaacgacg
     3781 tcgaccacgg catccagcgc gtcgacgcgt gccgcaccga ggacggggac ctgctgctgg
     3841 tggagctgga ggacctcaac ccgttcctct ccctggaccg ggtgccaccc gccacccgcg
     3901 acgcgttcgt ggcggcactc accgcgtcgc tgctgcgcat ggcgcgctga tcaccggcgc
     3961 agccgtacag gacgacggtc acgtccaccg tccttacata atcacggtca tggtcgaaca
     4021 gaatctctgg ctgcagaagg tcgcagacaa ccccgggcac tcctcgtggt acgtcgagcg
     4081 gttccggtcg atggcacgtg ccggtgacga tctcgccggt gaggcgcggc tggtggacgc
     4141 gatggtgccg cgccgcgcac ggatcctcga cgcggggtgc gggccgggtc gcgttggtgg
     4201 tgcgctggcc gaggcgggcc acgaggtcgt cggcgtcgac gtcgacccgg tgctcatcga
     4261 ggccgccgaa caggaccacc ccgggccgcg ctggctggtc ggtgacctgg ccgagctgga
     4321 cctgcccgcg agcggcgtca ccgaaccgtt cgacgcgctc gtctgcgcgg gcaacgtgat
     4381 gacgttcctc gcgcccggca cccgccggga ggtgctgacc cggttccgca cgcacctcgc
     4441 cgacgacggc cgggccgtga tcgggttcgg cgcggggcgc gggtacccgt tcgacgagtt
     4501 cctggccgac gccaaggctt ccggtctcgt gcccggtctg ctgctgtcca cgtgggacct
     4561 ccagccctac gccgaggacg cggacttcct cgtcgcgatc ctgcgcccgg cgagctgagc
     4621 gccgcgtacc cctggcgcgg ccggccattc cacagtggac gtggggtggc cgggtcaacg
     4681 cctgaaccag cgcctgcgcg ggcgttcgag gtggccctcg tcgtacagcc acgtctcgaa
     4741 gcggtccagg tcggcgcgga aggacagctc gacgggcttc gggtggtcgc gcgcccacgt
     4801 gtcgaacagc gggccgaacc ggtcctcgag caggtcggcc aggtcgggcc gcaggtggga
     4861 cgcgatcgag cggcgtttcg agcggagtgc ggccgcctcg acgccgaccc ggttggcgtc
     4921 gaagccatcg ggtaccgggc cgccggcaag cagcgcgcgg accagggcca gctgccgcgc
     4981 ggcgaggacg tccctggcgg tcatcgcccg cgcacggcca cggcgcggat cgcggccagt
     5041 tcggcggcga ggtcggcgtc ggcggggtag tcgtcgtcgc gttccagcag cacgcccggt
     5101 gggtcgacgc gggcgcacag ctcggccagc accgccagca cctccgacgg caccgcgtgc
     5161 gcgtgcgtgt cgtggtagac gccgtcgtgg gtgacaccgc ccgcgacgtg cacgtaggcc
     5221 agtcgttcca gcggcagttc gtcgaggaac accgcggggg ccgtgccgat gttgacggcg
     5281 ttggcgtaca ggttggccac gtcgacgagc agcaggcagc cggtgcggtc gaccagctcg
     5341 gcgaggaact ggccctcggt cagctcgttg tccggccagt cgaacaccgc cgcgatgttc
     5401 tccagcgcga ggggcaccgg caggtcggcc tgggcccgcc gcacgttgtc caccaggacg
     5461 ttcagcgcgt cgcgggtgcg cggcacgggg agcaggtgcc cggagtcgag cccgccggcg
     5521 cgggtgaacg cgacgtgctc gctgaccagc ggcgcgccga ccgccgccgc gaccgcggcc
     5581 aggtgctgga cccgggcggc gtcgaccggt tccgcaccgc cgagggacag cgacaccgcg
     5641 tgcgcgacca ccgggacacc gcgtgcccgc agcaccgtca acgactcggg cagcggtcca
     5701 gcatggatgt tctccgccag cacctccacg aagtcgacgc cgggcagcgc ttcgacggtc
     5761 aggtcgatct cgggccgcca gccgatgccg acaccgaggc ggggcgcgcc ggtcatcccg
     5821 agccgccgcc gcatccgccg ccaccaccac cgcaggacga gccgccgctg ccgcacgagg
     5881 acgacgacga ggacccgcac gagtgcccgc cgccaccacc ggacgagccg gaagagctgg
     5941 tcgcgggcac gtgggtggag aacgagccgg aggtggcata gctccacgcg accgggatga
     6001 gcgcgaccgg gacggccagg gcgagcgcgt gcgacaggtc cttgtcctcg atcacgcgtg
     6061 gaccggacac cgcggcccgc tccagctggc ccgcgccact cccccgccgc cgcacctcgg
     6121 ccaccacccg gtcgccgtgc acggtgcggg ccttgggttt ggtcaggttc ctggacccga
     6181 ggaagaacac cgcgatgagc gtcgcggcca gcaggaacgt gaggtagccg acgggcaggt
     6241 agttcccggc gccgttgatc cagcgcacga tccctgccac gaacaccacg tacagcagga
     6301 gcggcccgag ccgccgcgcc cggatcgcgg ccgagggcgc gaccagcagg ccgcgccgga
     6361 ccagcgactc gccgatcgcc gtgacctgct tctccaccgg cgcccgccgg acgatcgcgg
     6421 ccaccatgcg cggcttcgtg acctgcgcca gcacggcgtc gtccagtgga tgcccggtcg
     6481 acagggcccc ggacgtggcc gacacctggc cgctgcggtt cacccggacg agccccgcct
     6541 cggccagccg cgcgacggcg acctggaccg cgttgaccgg gcccccgccg aggaaaccca
     6601 gctcggtcac gtcgaggtgc ccgggcggct cggcgagccg cggcctgcgc accttcatgc
     6661 gcgcaccgat cgccacgacg agcccggcca ccagcccggc ggtgtagagc cagaggaact
     6721 ccggcccgga aagtccccac ggatgcatgc gccacccctc cccacggtcg tcgatggatc
     6781 atcgtcgacg cgggccggcg ggcaacgggt tacggggcgg ccgtgaccat cctgtcatct
     6841 gtccggcagc gccgtgcggt gcagctggcg cacgaggtcg aagcgcgggt cctcggcgcc
     6901 gggttggcgg gatgcgcgct ctcagccgtc gaggagcctg cggactacgg tgcggtcggc
     6961 gtcggtggtg gccatccgca acgcggtctc gtacgcggcg gcggcctcgt cgggcctgcc
     7021 gaggcggcgc aggaggttgc cgcgcacgga gtggtggagg tggtagcggt cgagcgccag
     7081 cgcgtcgacg aggtcgagtg cctccgccgg accgcgcagc tcggccacgg cgaccgcgcg
     7141 gttcagcgcc acgaccggcg tcggggccac gaccatcaac tggtcgtaca gcgcgacgac
     7201 ctgtgcccag tcggtctccc cggtcgtcgg ggcgtcgctg tgcacggcgt tgatcgcggc
     7261 ctggatctgg tagggccccg gcctgccgcg gcgcaggcac tgccgcacca gtgcgtgccc
     7321 ctcctcgacc atgccgcggt cccagcggcc gcggtcctgg tcgggcagca gcaccagctc
     7381 tccgtcgtcc gtgacccggg cggggcggcg cgactcgctg agcagcatga gcgcgagcag
     7441 gccggtgacc tccggttcgt cgggcatcag cgcggccagc acgcgaccga gccggatcgc
     7501 ctccgctgac agctcggggc ggtcgaccgt ggagtacccc tcgttgaaga tcaggtagag
     7561 gaccgcgagc acaccgccga ggcgggcggg gaggtcggcc tcgtacggga cgcggtacgg
     7621 gatgcccgcg tcgcggatct tgttcttggc gcggaccagc cgctgcgcca tggtcggttc
     7681 tggcacgagg aacgcgcggg cgacctcctc ggtcgtcaga ccgccaagga gccgcagggt
     7741 cagcgccacc cgcacaccca tggccagcga cgggtggcag caggtgaaga tcaggcgcag
     7801 ccggtcgtcg cgcaccacgc cctcctcacc ttcctcgtct ccgcgctcgg ctgccagcag
     7861 ttccagggcc tgccggtgcc gttcctcgcg ggtggcctcg cggcgcaggc ggtcgatcgc
     7921 gcggttgcgg gcggtcgtga tgagccagcc ggccgggctc ggcggcaccc cgtccgccgg
     7981 ccagcggcgg accgcttcga cgaacgcgtc ctgcacgacc tcttccgcga gcccgatgtc
     8041 accaagggac cggacgagca cggacaccgc ccgcccgtac tcgcgccgga agacgccgtc
     8101 gaggttcacc ggcgtggcgc gtacatgatc acggccaccc cggccaggca cagtgcggcg
     8161 ccgacgtagt cccaccgatc gggctggaac ttgtcgacga cgacgcccca cgcgagcgac
     8221 ccggcgacga acaccccgcc gtacgcggcg aggatccgcc cgaagtgcgg gtccggctgg
     8281 aacgtggcga cgaacccgta cgcgccgagc gcgacgaccc cggcggcgat ccaccacagt
     8341 ccgcggtcct cgcgccagcc ctgccagacc agccacgcac cgccgatctc ggcgagcgcc
     8401 gccagcacga acaacacgag cgacttggca acggtcacct ctggttcacc acacaagctc
     8461 cgctcactga catggtcgct acgcaagctc cgctcactga catggtcgct acgcaagctc
     8521 cgctcactgg aacggacgca cctcgatcgc caacggcgac agcaccgccg cgagcttgcg
     8581 tccccactcc atcgcggcgt cgaggtcctc ggcccggatg accgtgaacc cgccgaggaa
     8641 ctccttggcc tcgatgaacg gcccgtcggt cacgagcacc tcctcgccgg tggcgcgcag
     8701 gacggtcgcg gtctccggcg ggtgcagccc ggccgcgaac acccacacac ccgcggcccg
     8761 catctccttg ttgagcgcgt ccaggttccg catgacgggc tcgagagctt ctggctccgg
     8821 ggtgccgtcg ggctggtaga tgctcagcag gtactgcttc atggcctgat ccttccgcag
     8881 acagccggtc accacccaca cgaacgcgcc ctccgcacat cgacacccaa tgtactatgg
     8941 tttccataca ttcttgatct cgtatggagg cctcgtgtac ctgatcaccg gcgcgaccgg
     9001 cactgtcggc cgcccactgc tggcggagct ggacggccac gcggtgcgtc ccgtcacccg
     9061 cgacccgtcc cgtctgcccg gcgcggtggc cgagcccgac gtcaccggtg tcaccgcggt
     9121 gttcctgcac ccgcgcgcgg tgggactcgg cgccgcggac ctgctggtcc gcgcgaaggc
     9181 ggcgggggtg cggcgggtcg tcgtgctgtc ggccgtcaac gtcgacgacc cgctcgacga
     9241 gcagccgtcc cgtgccaacg gcgaccgcga cacggaggtc gaggcggccg cgatcggcag
     9301 cggtctcgag tgggtgagcg cggtcgcggc ggcgggcatg gtcgagcacg gtcaccgccc
     9361 ggagttcgtc gccgcgctga tggcacggta cgaacgggag aacggcaggc ccgcccacgt
     9421 gagcggggac gtcgagaatg tgctgggccg cccggcgcgc tcgttcgccg agtgggtcgc
     9481 cgaccacgcc gagtacgtcc gatgaccgtc gtccacatcg ggctcgtgtt ggtcgccatt
     9541 gcggccaacg ccttttccgc ggtcgccgac ttcctccgcc tggagcggat ccggggcggc
     9601 atgacacggg tcggggtgcc gacgcggatc ctgccctggc tgggcgtgcc caaggcgctc
     9661 gccgtcgccg ggttgctcgc tgggctggcc gtgccgtggc tcgcggtggc ggccgcggcc
     9721 ggcctcgtgc tgttcttcct cctggcgatc ggcacgcacc tgcgggcggg tgaccgcatg
     9781 atcgggctgc cactcggctt cctcgccctc gcggtggcgg tggtggctac ggcaccggtg
     9841 tgagcagtgt gccgtcggtg aggaactggt gcaggttccg gaacgccagg tcgcccatcg
     9901 cctcgcgggt ctccacggtg gcgctcgcga tgtgcggcag cagcaccacg ttgtccagcc
     9961 ccagcaacgg ttccggcacg ttcggctcgt ccgcgaacac gtccagcgcg gcgccggcga
    10021 tgccgccggt cgtcagcgcg gccaccagcg cgggctcgtc gacgacactg ccccgcgcga
    10081 tgttcacgag gtaacccgcc gggccgagcg cggccagcac gtccgaggag acgagaccgg
    10141 cggtcgccgg gccacccgac gtcgccacca ccagcacctc gaccgcctcg gccagtgctg
    10201 ccggggagtc gtggtaggtg tacggcaggc caggcacctg gtggcgggag tggtagctca
    10261 gccgcgcgtc gaacgcggcc agccggtgcg cgatcgcgcg gccgatgcgg cccagtccga
    10321 ggatgcccac gcggcggccg gtcaccttgg tggccaacgg gaactgcccg cgccgccagt
    10381 cgccgcggcg gacgaaccgg tccgccgcgg tgagcctgcg cagcacgtcg atcatgccgc
    10441 cgaccgccag gtcggccacg cagtcggtca gcacgtcggg tgtgttgctg acgacgatgc
    10501 cgcgggagcg cgcgtacggc acgtccgtgg tctcgtagcc gactccgaag tggacgatcg
    10561 cgcgcagcga cggcagcgcg tcgatctgct cgttggtcac cccgaaccgg gacgaggtga
    10621 tcgcggcgac cacgtcgtcg gacggctcgt ccacgatctt gtggttcgtc cgcaggcgct
    10681 cctccagcga gggcagcagc ggacatgcct gccataccgg acgggtcacg ccgcttggct
    10741 ccgttcccgt ggtcacgtgg tctccaacgc gggtcgtttc gggtcgaacc gccaccccgg
    10801 cacgaggtac cgcatggcga cggcgtcgtc ccgg
//
