LOCUS       NZ_JACHJK010000051      1502 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Streptomyces echinatus strain CECT 3313 Ga0436950_51, whole genome
            shotgun sequence.
ACCESSION   NZ_JACHJK010000051
VERSION     NZ_JACHJK010000051.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces echinatus
  ORGANISM  Streptomyces echinatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 05:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,466
            CDSs (total)                      :: 8,381
            Genes (coding)                    :: 8,094
            CDSs (with protein)               :: 8,094
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 5, 1 (5S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 116 of 287
            Pseudo Genes (incomplete)         :: 236 of 287
            Pseudo Genes (internal stop)      :: 12 of 287
            Pseudo Genes (multiple problems)  :: 77 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1502
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJK010000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     aSDomain        complement(<1..587)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41820_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.30E-70"
                     /label="FHS34_RS41820_Condensation_LCL.1"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="500"
                     /protein_start="303"
                     /score="229.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAADG"
                     /tool="antismash"
                     /translation="EQLPLSFAQRRLWFLHRFEGPSATYNVPMRLRLQTQPDTDALVSA
                     IGDVVRRHESLRTVFTEIDGTPVQRILPAETADVPVEVLQATTAAELETLVTQVARYEF
                     DLETEIPIRARVINTDDDQCTLILVVHHIAADGWSLVPLMRDLSDAYTARRNGHQPDWE
                     PLAVQYADYTLWQRDVLGTEDDPDSVLSQQFAYW"
     CDS             complement(<1..1498)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (222-290). E-value: 7.7e-25. Score:
                     78.9. Matches aSDomain: nrpspksdomains_FHS34_RS41820_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (303-500). E-value:
                     1.3e-70. Score: 229.8. Matches aSDomain:
                     nrpspksdomains_FHS34_RS41820_Condensation_LCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="FHS34_RS41820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_008499"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_245002642.1"
                     /sec_met_domain="AMP-binding (E-value: 4.5e-10, bitscore:
                     24.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.6e-18, bitscore:
                     51.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.3e-46, bitscore:
                     145.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLFQTSLAFQNNTLPHLTLGNINTTYEPTSTGTSRFDLLFNIVEP
                     SDDENGNAGYRGMVEYATDLFDKETIEQLVTRYLSLLKTAVANPDAPLRTLDIFLPHER
                     HQLLETWSRQAEVLGHEAVQSLVEEVQDARVHVLDGELGLVPSGVVGDLYVSGVPSDRE
                     GTTAGHFETDPYGPSGSRLYRTDRSARWTRDGRLELVRRADDKAAGQVVPGYRKPRNVQ
                     EEVLVGLFAEVLNVDRVGIDDSFFGLGGHSLLATWLLSRIRTVLGVEVPIRMFFEYPTV
                     ARLAPQLDRGPVQIPVVARARPEQLPLSFAQRRLWFLHRFEGPSATYNVPMRLRLQTQP
                     DTDALVSAIGDVVRRHESLRTVFTEIDGTPVQRILPAETADVPVEVLQATTAAELETLV
                     TQVARYEFDLETEIPIRARVINTDDDQCTLILVVHHIAADGWSLVPLMRDLSDAYTARR
                     NGHQPDWEPLAVQYADYTLWQRDVLGTEDDPDSVLSQQFAYW"
     source          1..1502
                     /db_xref="taxon:67293"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces echinatus"
                     /strain="CECT 3313"
                     /submitter_seqid="Ga0436950_51"
                     /type_material="type strain of Streptomyces echinatus"
     gene            complement(<1..>1502)
                     /locus_tag="FHS34_RS41820"
                     /old_locus_tag="FHS34_008499"
     region          1..1502
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1502
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1502
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1498](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1498)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(48..86)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41820_0005"
                     /evalue="1.60E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="483"
                     /protein_start="470"
                     /score="17.4"
                     /tool="antismash"
                     /translation="QYADYTLWQRDVL"
     CDS_motif       complement(135..200)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41820_0004"
                     /evalue="3.60E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="454"
                     /protein_start="432"
                     /score="35.5"
                     /tool="antismash"
                     /translation="VHHIAADGWSLVPLMRDLSDAY"
     CDS_motif       complement(408..521)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41820_0003"
                     /evalue="1.90E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="363"
                     /protein_start="325"
                     /score="46.0"
                     /tool="antismash"
                     /translation="ATYNVPMRLRLQTQPDTDALVSAIGDVVRRHESLRTVF"
     CDS_motif       complement(540..572)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41820_0002"
                     /evalue="1.00E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="319"
                     /protein_start="308"
                     /score="11.4"
                     /tool="antismash"
                     /translation="SFAQRRLWFLH"
     aSDomain        complement(627..830)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41820_PCP.1"
                     /evalue="7.70E-25"
                     /label="FHS34_RS41820_PCP.1"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="290"
                     /protein_start="222"
                     /score="78.9"
                     /tool="antismash"
                     /translation="EEVLVGLFAEVLNVDRVGIDDSFFGLGGHSLLATWLLSRIRTVLG
                     VEVPIRMFFEYPTVARLAPQLDR"
     CDS_motif       complement(1104..1139)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41820_0001"
                     /evalue="6.10E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="FHS34_RS41820"
                     /protein_end="131"
                     /protein_start="119"
                     /score="-2.8"
                     /tool="antismash"
                     /translation="HEAVQSLVEEVQ"
ORIGIN
        1 ccagtaggcg aattgctgtg acaggacgga gtctgggtcg tcttcggtgc cgaggacgtc
       61 gcgttgccag agggtgtagt cggcgtattg gacggcgagg ggttcccagt cgggttggtg
      121 tccgttgcgg cgtgcggtgt aggcgtcgga caggtcacgc atcagcggga cgagtgacca
      181 tccgtcggcg gcgatgtggt ggacgaccag gatcagtgtg cactggtcgt cgtcggtgtt
      241 gatgacgcgg gcgcgtatcg ggatctctgt ttccagatcg aattcatacc gggcgacctg
      301 ggtgaccaat gtttccaact cggccgcggt ggtggcttgc agcacctcga cagggacgtc
      361 tgccgtttcg gcgggcagta tgcgctgcac cggtgtgccg tcaatttcgg tgaagacggt
      421 gcgcaggctt tcgtggcgcc gcaccacgtc gccgatcgcg gacaccaatg cgtccgtgtc
      481 gggttgggtc tgcaagcgaa gtcgcatcgg cacgttgtag gtggccgagg gtccctcgaa
      541 ccggtgcagg aaccacagtc ggcgctgcgc gaaggacaac ggcagctgtt ccgggcgggc
      601 ccttgccacc accgggatct gcaccggccc cctgtccagc tgcggtgcca gccgcgccac
      661 cgtcggatac tcgaagaaca tccggatcgg cacctcgacg ccgagtacgg tccggatgcg
      721 ggacaacaac cacgtggcca gcagcgagtg gccgcccaga ccgaagaagc tgtcgtcgat
      781 cccgaccctg tcgacgttca gcacctccgc gaacagcccg accaggactt cctcctgaac
      841 gttgcgcggc ttccggtacc ccggcacgac ttgcccagcc gccttgtcgt cggctcgtcg
      901 caccaactcc agccggccgt cgcgagtcca ccgggccgac cgatcggtgc ggtacagccg
      961 ggatccggac gggccgtagg ggtccgtctc gaagtgcccg gcggtcgttc cctcccgatc
     1021 cgacggcaca cccgacacgt acaggtcgcc caccacaccg ctgggtacca gccccaactc
     1081 gccgtccagc acgtggaccc gtgcgtcctg gacttcttcc accagtgact ggaccgcttc
     1141 atgaccaagg acctcggctt gccgactcca ggtttcgagg agttggtggc gttcgtgcgg
     1201 gaggaatatg tcgagggttc gcaggggcgc gtccgggttg gcgacggctg ttttgagcag
     1261 gctgagatag cgggtgacga gctgttcgat ggtttccttg tcgaacaggt cggtggcgta
     1321 ttcgaccatt cctcggtagc cggcgttccc gttctcatcg tccgagggtt cgacgatgtt
     1381 gaagaggagg tcgaatcgtg aggtgccggt tgaggtgggt tcgtaggtgg tgttgatgtt
     1441 gccgagggtg aggtggggga gggtgttgtt ctggaaggcg agggaggttt ggaagagggg
     1501 gt
//
