LOCUS       NZ_JACHJK010000049      1855 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Streptomyces echinatus strain CECT 3313 Ga0436950_49, whole genome
            shotgun sequence.
ACCESSION   NZ_JACHJK010000049
VERSION     NZ_JACHJK010000049.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces echinatus
  ORGANISM  Streptomyces echinatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 05:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,466
            CDSs (total)                      :: 8,381
            Genes (coding)                    :: 8,094
            CDSs (with protein)               :: 8,094
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 5, 1 (5S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 116 of 287
            Pseudo Genes (incomplete)         :: 236 of 287
            Pseudo Genes (internal stop)      :: 12 of 287
            Pseudo Genes (multiple problems)  :: 77 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1855
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJK010000049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..1855
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     gene            complement(<1..1840)
                     /locus_tag="FHS34_RS41810"
                     /old_locus_tag="FHS34_008497"
     CDS             complement(<1..1840)
                     /NRPS_PKS="Domain: AMP-binding (222-613). E-value:
                     9.3e-103. Score: 335.9. Matches aSDomain:
                     nrpspksdomains_FHS34_RS41810_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="FHS34_RS41810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_008497"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_245002641.1"
                     /sec_met_domain="Condensation (E-value: 1.3e-13, bitscore:
                     36.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.9e-98, bitscore:
                     315.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVLQAGLAVLLKRLGAGSDIPLGSPIAGRTDEALDDLVGFFVNTL
                     VLRTDVSGHPTFRELLARVRETDLAAYAHQDVPFEHLVEVLNPTRSLSHHPLFQVMLAL
                     QNTPQGAFALPGLDSRIEPVGTGTSRFDLFLNLRERTSPTGTPAGLEGFVEYSTDLFDQ
                     STVTTLIERLTRVLEQAVARPDAPITRAELLSTDERHQLVAEWNATQRDVVLPRVLSEA
                     FEAQVRKTPEAPAVVFETTELSYAELNERANRLARLLITHGAAPDRLVALAVPRSAQMI
                     VAVLAVLKTGAAYLPLDVEYPAERLAFMVQDARPVLMLTTTAAAGHLPEDTPRLLLDDP
                     AVTTALAELPATDIDPGERHSAVTPQSPAYVIYTSGSTGTPKGVIVTQESVTDLLSWAV
                     TTFDDDRLTHVLAATSLSFDVSVFELFAPLVSGGTIEVVRDILALLGRNWTGSLISAVP
                     SALAQVIAQPGLTVQARTVVLAGEALPTQTVTDIHNALPGTHITNIYGPTEATVYATMS
                     GPLTGTHTPPIGRPITNTQAFVLDAGLAPVPAGVVGELYLAGTGLARGYLNRPALTAER
                     FVANPFGGPGERMYRTGDLVRWNTSGELEYIGRADTQ"
     source          1..1855
                     /db_xref="taxon:67293"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces echinatus"
                     /strain="CECT 3313"
                     /submitter_seqid="Ga0436950_49"
                     /type_material="type strain of Streptomyces echinatus"
     region          1..1855
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     protocluster    1..1855
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1840](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1840)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        complement(2..1174)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41810_AMP-binding.1"
                     /evalue="9.30E-103"
                     /label="FHS34_RS41810_AMP-binding.1"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="613"
                     /protein_start="222"
                     /score="335.9"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FEAQVRKTPEAPAVVFETTELSYAELNERANRLARLLITHGAAPD
                     RLVALAVPRSAQMIVAVLAVLKTGAAYLPLDVEYPAERLAFMVQDARPVLMLTTTAAAG
                     HLPEDTPRLLLDDPAVTTALAELPATDIDPGERHSAVTPQSPAYVIYTSGSTGTPKGVI
                     VTQESVTDLLSWAVTTFDDDRLTHVLAATSLSFDVSVFELFAPLVSGGTIEVVRDILAL
                     LGRNWTGSLISAVPSALAQVIAQPGLTVQARTVVLAGEALPTQTVTDIHNALPGTHITN
                     IYGPTEATVYATMSGPLTGTHTPPIGRPITNTQAFVLDAGLAPVPAGVVGELYLAGTGL
                     ARGYLNRPALTAERFVANPFGGPGERMYRTGDLVRWNTSGELEYIGRADTQ"
     CDS_motif       complement(110..199)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0007"
                     /evalue="2.60E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="577"
                     /protein_start="547"
                     /score="58.1"
                     /tool="antismash"
                     /translation="PAGVVGELYLAGTGLARGYLNRPALTAERF"
     CDS_motif       complement(197..226)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0006"
                     /evalue="8.20E+00"
                     /label="NRPS-A_a2"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="548"
                     /protein_start="538"
                     /score="0.9"
                     /tool="antismash"
                     /translation="VLDAGLAPVP"
     CDS_motif       complement(680..736)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0005"
                     /evalue="4.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="387"
                     /protein_start="368"
                     /score="31.3"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGTPKGVIVT"
     CDS_motif       complement(947..988)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0004"
                     /evalue="1.00E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="298"
                     /protein_start="284"
                     /score="18.6"
                     /tool="antismash"
                     /translation="LAVLKTGAAYLPLD"
     CDS_motif       complement(1529..1648)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0003"
                     /evalue="1.30E-24"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="104"
                     /protein_start="64"
                     /score="78.7"
                     /tool="antismash"
                     /translation="RETDLAAYAHQDVPFEHLVEVLNPTRSLSHHPLFQVMLAL"
     CDS_motif       complement(1697..1786)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0002"
                     /evalue="2.90E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="48"
                     /protein_start="18"
                     /score="48.6"
                     /tool="antismash"
                     /translation="DIPLGSPIAGRTDEALDDLVGFFVNTLVLR"
     CDS_motif       complement(1811..1837)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41810_0001"
                     /evalue="7.80E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="FHS34_RS41810"
                     /protein_end="10"
                     /protein_start="1"
                     /score="-2.0"
                     /tool="antismash"
                     /translation="VLQAGLAVL"
ORIGIN
        1 cctgggtgtc ggcccggccg atgtattcga gttcgccgga ggtgttccag cggaccaggt
       61 caccggtgcg gtacatgcgc tcgcccgggc cgccgaaggg gttggcgacg aagcgttcgg
      121 cggtcagggc cgggcggttg aggtagccgc gggccagacc ggtgcctgcc aggtacagct
      181 cacccaccac acccgcgggt acgggggcga gtccggcgtc gaggacgaag gcttgggtgt
      241 tggtgatggg gcggccgatg gggggtgtgt gtgttccggt gagggggccg ctcatggtgg
      301 cgtagacggt ggcttcggtg gggccgtaga tgttggtgat gtgggtgccg gggagggcgt
      361 tgtggatgtc ggtgacggtt tgggtgggga gtgcttcgcc ggcgaggacg acggttcggg
      421 cttggacggt gaggccgggt tgtgcgatga cttgggcgag ggcggagggt actgcgctga
      481 tgaggcttcc ggtccagttg cgtccgagga gggcgaggat gtcgcggacg acttcgatgg
      541 tgccgcctga gacgaggggg gcgaagagtt cgaagacgga gacgtcgaag ctgagggagg
      601 tggcggccag gacgtgggtg aggcggtcgt cgtcgaaggt ggtcactgcc caggagagga
      661 ggtcggtgac gctttcctgg gtgacgatga cgcccttggg ggtgccggtg gagccggagg
      721 tgtagatgac ataggcgggg gactgcgggg tgacggcgct gtggcgttcg ccggggtcga
      781 tgtcggtggc cgggagctcg gcgagcgccg tggtcaccgc cgggtcgtcc aggagcagcc
      841 gcggggtgtc ttccggcagg tggccggccg ccgccgtggt cgtcagcatc aggaccggtc
      901 gggcgtcctg caccatgaag gcgaggcgct cggccgggta ttcgacgtcc aggggcaggt
      961 aggcggcgcc ggttttgagg acggcgagga cggcgacgat catttgggcg gagcgtggca
     1021 cggccagcgc gaccaggcgg tcgggtgcgg cgccgtgggt gatgagcagg cgtgcgaggc
     1081 ggttggcccg ctcgttcagc tcggcgtagg acagctcggt ggtctcgaag acgacggcgg
     1141 gtgcttcggg tgtcttccgc acctgggctt cgaaggcctc ggacagtacg cggggcagta
     1201 cgacgtcgcg ctgggtggcg ttccactcgg cgaccagctg gtggcgctcg tcggtggaga
     1261 ggagctcggc gcgtgtgatg ggtgcgtcgg gccgggcgac ggcctgttcc aggacgcggg
     1321 tgaggcgttc gatgagggtg gtgacggtgc tctggtcgaa gaggtcggtg ctgtattcga
     1381 cgaagccttc gaggccggcg ggtgtgccgg tgggggaggt tcgttcgcgc aggttgagga
     1441 agaggtcgaa acgggaggtg ccggtgccga ccggttcgat gcggctgtcc agtccgggca
     1501 gtgcgaaggc gccctggggc gtgttctgca gtgccagcat gacttggaag agggggtggt
     1561 gggagaggga gcgggtgggg ttgaggactt cgacgaggtg ttcgaagggg acgtcctggt
     1621 gggcgtaggc ggcgaggtcg gtttcgcgga cgcgggcgag gagttcgcgg aaggtggggt
     1681 ggccggagac gtcggtgcgc aggacgaggg tgttgacgaa gaagccgacg aggtcgtcga
     1741 gggcttcgtc cgtgcggccc gcgatgggtg agcccagcgg gatgtcggat cccgcgccca
     1801 ggcgcttgag cagcaccgcg agaccggcct gcagcaccat gaacacactc gcccc
//
