LOCUS       NZ_JACHJK010000046      2030 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Streptomyces echinatus strain CECT 3313 Ga0436950_46, whole genome
            shotgun sequence.
ACCESSION   NZ_JACHJK010000046
VERSION     NZ_JACHJK010000046.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces echinatus
  ORGANISM  Streptomyces echinatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 05:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,466
            CDSs (total)                      :: 8,381
            Genes (coding)                    :: 8,094
            CDSs (with protein)               :: 8,094
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 5, 1 (5S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 116 of 287
            Pseudo Genes (incomplete)         :: 236 of 287
            Pseudo Genes (internal stop)      :: 12 of 287
            Pseudo Genes (multiple problems)  :: 77 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2030
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJK010000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     aSDomain        complement(<1..830)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41790_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.60E-101"
                     /label="FHS34_RS41790_Condensation_LCL.1"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="677"
                     /protein_start="400"
                     /score="329.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHITGDG"
                     /tool="antismash"
                     /translation="ETAPLSFSQRRLWFLHRLEGPSATYNMPMAIRLTGELDRAALHDG
                     LTDVLARHESLRTVFPEVDGVPRQKVLSVSEAGVELSVVPTTEEELAARLADASAEGFD
                     LANDLPLRVTLFVLSPTEHVLVLVLHHITGDGWSLRPLSRDVGEAYAARVEGRAPEWPE
                     LPVQYVDYTLWQQELLGAEDDPQSVISRQVDYWKTTLADIPERLDVPVDRPRPAATTYR
                     GDLVDIEMSAELHGRIVALARECGASVFMVLQAGLAVLLKRLGAGSDIPLGSPIA"
     source          1..2030
                     /db_xref="taxon:67293"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces echinatus"
                     /strain="CECT 3313"
                     /submitter_seqid="Ga0436950_46"
                     /type_material="type strain of Streptomyces echinatus"
     gene            complement(<1..>2030)
                     /locus_tag="FHS34_RS41790"
                     /old_locus_tag="FHS34_008493"
     region          1..2030
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2030
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2030
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2030](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2030)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..2030)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (317-384). E-value: 4.2e-30. Score:
                     95.7. Matches aSDomain: nrpspksdomains_FHS34_RS41790_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (400-677). E-value:
                     7.6e-101. Score: 329.0. Matches aSDomain:
                     nrpspksdomains_FHS34_RS41790_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099845.1"
                     /locus_tag="FHS34_RS41790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_008493"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_184976185.1"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-64, bitscore:
                     204.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-19, bitscore:
                     56.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.4e-60, bitscore:
                     189.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MILETLGTGKRLVVPDTDVRRDPTRFVQWLDEHRIEELYAPNVMV
                     QAVCEAALEQGRTLPHLRHIAQAGEALQLSPATKEFFAGRPERVLHNHYGPAETHVITT
                     YSLPGEVSDWGTTAPIGGPLWNTRVFVLDDALAPVPAGVTGELYLAGTALARGYWDRPG
                     LTAERFVANPYGGPGERMYRTGDLVRWNTAGELEYIGRADNQVKVRGFRIELGEIETAL
                     TGQESVAQAAVVVREDRPGDRRVVAYVVPASGTVAQPSALRRQLAQALPDYMVPSAVVV
                     LDALPLTPSGKVDRRALPAPALDVSPSGHAAPRTPQEEILCGIFAEVLGVGQVGVDDSF
                     FDLGGHSLLATRLVSRVRSVLDVELSVRAVFEAPTVAALVQRLPNAGIARKALRAAVER
                     PETAPLSFSQRRLWFLHRLEGPSATYNMPMAIRLTGELDRAALHDGLTDVLARHESLRT
                     VFPEVDGVPRQKVLSVSEAGVELSVVPTTEEELAARLADASAEGFDLANDLPLRVTLFV
                     LSPTEHVLVLVLHHITGDGWSLRPLSRDVGEAYAARVEGRAPEWPELPVQYVDYTLWQQ
                     ELLGAEDDPQSVISRQVDYWKTTLADIPERLDVPVDRPRPAATTYRGDLVDIEMSAELH
                     GRIVALARECGASVFMVLQAGLAVLLKRLGAGSDIPLGSPIA"
     CDS_motif       complement(294..332)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0007"
                     /evalue="2.60E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="579"
                     /protein_start="566"
                     /score="13.7"
                     /tool="antismash"
                     /translation="QYVDYTLWQQELL"
     CDS_motif       complement(381..446)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0006"
                     /evalue="1.60E-07"
                     /label="C3_LCL_132-143"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="550"
                     /protein_start="528"
                     /score="23.9"
                     /tool="antismash"
                     /translation="LHHITGDGWSLRPLSRDVGEAY"
     CDS_motif       complement(648..764)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0005"
                     /evalue="1.50E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="461"
                     /protein_start="422"
                     /score="59.2"
                     /tool="antismash"
                     /translation="ATYNMPMAIRLTGELDRAALHDGLTDVLARHESLRTVFP"
     CDS_motif       complement(783..815)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0004"
                     /evalue="4.50E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="416"
                     /protein_start="405"
                     /score="9.7"
                     /tool="antismash"
                     /translation="SFSQRRLWFLH"
     aSDomain        complement(879..1079)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41790_PCP.1"
                     /evalue="4.20E-30"
                     /label="FHS34_RS41790_PCP.1"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="384"
                     /protein_start="317"
                     /score="95.7"
                     /tool="antismash"
                     /translation="EEILCGIFAEVLGVGQVGVDDSFFDLGGHSLLATRLVSRVRSVLD
                     VELSVRAVFEAPTVAALVQRLP"
     CDS_motif       complement(1134..1160)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0003"
                     /evalue="2.90E-02"
                     /label="NRPS-A_a10"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="299"
                     /protein_start="290"
                     /score="7.1"
                     /tool="antismash"
                     /translation="GKVDRRALP"
     CDS_motif       complement(1374..1439)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0002"
                     /evalue="8.00E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="219"
                     /protein_start="197"
                     /score="40.2"
                     /tool="antismash"
                     /translation="YIGRADNQVKVRGFRIELGEIE"
     CDS_motif       complement(1524..1613)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41790_0001"
                     /evalue="3.90E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="FHS34_RS41790"
                     /protein_end="169"
                     /protein_start="139"
                     /score="51.1"
                     /tool="antismash"
                     /translation="PAGVTGELYLAGTALARGYWDRPGLTAERF"
ORIGIN
        1 gcgatgggtg agcccagcgg gatgtcggat cccgcgccca ggcgcttgag cagcaccgcg
       61 agaccggcct gcagcaccat gaacacactc gccccacatt cacgggccag tgcgacgatc
      121 cgcccgtgca gctcggcgct catctcgatg tcgacgagat cgcctcgata cgtggtcgcc
      181 gccggacgcg gacggtccac cgggacgtcc aaccgctcgg gtatgtcggc cagggtcgtc
      241 ttccagtagt cgacctgtcg gctgatgacg ctctgcggat cgtcctcggc ccccagcagc
      301 tcctgctgcc acaacgtgta gtccacgtac tgcaccggaa gctccggcca ctcgggcgcc
      361 cggccttcca cccgtgccgc gtacgcctcg cccacgtccc gtgacagggg ccgcagggac
      421 cagccgtcgc ccgtgatgtg gtgcagcacc agtaccagca cgtgctcggt cggcgacagc
      481 acgaacagcg tcacccgcaa cgggaggtcg ttcgccagat cgaagccttc ggcggacgcg
      541 tcggcgagcc tcgccgcgag ctcctcctcc gtcgtcggca ccaccgacag ctcgaccccg
      601 gcttcggaga cgctcagcac cttctgccgc ggcaccccgt cgacctcggg gaagaccgtc
      661 cgcaggctct catgacgcgc gagcacgtcg gtgagaccgt cgtgcagggc ggcgcggtcg
      721 agttcgccag tcagccggat ggccatgggc atgttgtagg tcgcgctggg tccttccagt
      781 cggtggagga accacaggcg ccgctgcgag aaggacaacg gagccgtctc cgggcgttcc
      841 acggccgccc gcagcgcctt cctggcgata ccggcgttcg gcaggcgctg taccagagcg
      901 gcgacggtgg gtgcctcgaa gaccgcgcgg acggagagtt ccacgtcgag taccgagcgg
      961 acgcggctga cgagtcgggt ggccagcagg gagtgtccgc cgaggtcgaa gaagctgtcg
     1021 tcgacaccga cctggcccac acccagcacc tcggcgaaga tgccgcacag gatctcctcc
     1081 tgtggtgtcc gcggggcggc atgtcccgag ggcgagacgt ccagggccgg cgcggggagt
     1141 gcccggcggt ccaccttgcc gctgggggtg agcggcagcg cgtccagcac gacgaccgcc
     1201 gagggcacca tgtagtcggg gagtgcctgt gcgagctgcc ggcgcagggc ggagggctga
     1261 gcgacggtgc cggatgcggg gacgacgtag gcgacgaccc gccggtcgcc gggacggtct
     1321 tcgcgtacca cgaccgcggc ctgggccacg ctttcctggc cggtgagggc tgtttcgatc
     1381 tcgccgagtt cgatgcggaa gccgcggacc ttgacctggt tgtcggcccg gccgatgtac
     1441 tccagctctc ccgcggtgtt ccagcggacc aggtcaccgg tgcggtacat gcgctcaccc
     1501 ggaccgccgt aggggttcgc gacgaagcgt tccgcggtga ggccggggcg gtcccagtag
     1561 ccgcgggcca gggcggtgcc cgcgaggtac agctcgccgg tcactccggc cggcaccggt
     1621 gcgagcgcgt cgtccaggac gaacacgcgg gtgttccaga gggggccgcc gatcggtgcc
     1681 gtcgtacccc agtccgacac ctcgcccggc agggagtacg tggtgatgac gtgcgtctcc
     1741 gcggggccgt agtggttgtg gagcacacgc tccgggcggc ccgcgaagaa ctccttcgtc
     1801 gccgggctca gctggagggc ctcgcccgcc tgggcgatgt gacggaggtg ggggagggta
     1861 cggccctgct ccagggccgc ctcgcacacc gcctggacca tgacgttcgg tgcgtacagc
     1921 tcctcgatgc ggtgttcgtc cagccactgc acgaaccgtg tggggtcgcg tcgtacgtcc
     1981 gtgtcgggga cgacgagtcg tttgccggtg ccgagggttt cgaggatctc
//
