LOCUS       NZ_JACHJK010000045      2046 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Streptomyces echinatus strain CECT 3313 Ga0436950_45, whole genome
            shotgun sequence.
ACCESSION   NZ_JACHJK010000045
VERSION     NZ_JACHJK010000045.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces echinatus
  ORGANISM  Streptomyces echinatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 05:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,466
            CDSs (total)                      :: 8,381
            Genes (coding)                    :: 8,094
            CDSs (with protein)               :: 8,094
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 5, 1 (5S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 116 of 287
            Pseudo Genes (incomplete)         :: 236 of 287
            Pseudo Genes (internal stop)      :: 12 of 287
            Pseudo Genes (multiple problems)  :: 77 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2046
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJK010000045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2046
                     /db_xref="taxon:67293"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces echinatus"
                     /strain="CECT 3313"
                     /submitter_seqid="Ga0436950_45"
                     /type_material="type strain of Streptomyces echinatus"
     gene            <1..>2046
                     /locus_tag="FHS34_RS41785"
                     /old_locus_tag="FHS34_008492"
     region          1..2046
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2046
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2046
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>2046](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2046
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     CDS             1..>2046
                     /NRPS_PKS="Domain: AMP-binding (114-500). E-value: 3e-99.
                     Score: 324.3. Matches aSDomain:
                     nrpspksdomains_FHS34_RS41785_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (605-674). E-value: 2.1e-26. Score:
                     83.9. Matches aSDomain: nrpspksdomains_FHS34_RS41785_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="FHS34_RS41785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_008492"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_184976181.1"
                     /sec_met_domain="AMP-binding (E-value: 3.1e-111, bitscore:
                     357.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.5e-17, bitscore:
                     48.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPHLTLGNINTTYEPTSTRTSRFDLLFNIVEPSHDSAAQPGYAGH
                     VEYATDLFDKETIEQLITRFKTVLRTVVATPDTPLRTLDVLLPDERHRLLEAWGRTSTA
                     ASITTVPGLIENMAARMPDAIAVTSADGELTYRQLDERADHIAHALLAQGVHLESVVAV
                     ALPRSPELITAILGILKAGAAYLPIDPAYPADRVESVLSDASPAVVVTDSATAGSVVGN
                     GLRHLLVDEVTAAGAHPRPALLPDNLAYLMYTSGSSGVPKGVGITHASVTLCAQELATR
                     FDIKPGGITLASTSVSFDVSVFEIVATLASGGTVDMVQDVLEFARRDTWAGSVVSTVPS
                     AFAELLNDLPESITIDTVVLAGEALPADLVRRLRQALPETRIFNAYGQTESFYATTTGP
                     LSAMDERSNAPIGTPLGGVHVYVLDPDLRLVPRGVAGELYVAGPNIGRGYHALPGLTAP
                     RFVADPFGVAGARMYRTGDLVRWTRDGELVFVGRADDQVKIRGFRVEPGEVEAVLGSHP
                     HVSRAVVVARDGVGGTQLVAYAVGVGSGPPDGEELRRFLASRLPEFMVPAAVMVLDELP
                     LTVNGKLDRRALPEPEFLGDAYRAPRNLHEEMLAGLFAEVLEVDQVGIDDGFFALGGHS
                     LLATRLTSRIRSVLGFEVPIRAVFENPTVAGLTPWLTHNRPVQIPVT"
     aSDomain        343..1500
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41785_AMP-binding.1"
                     /evalue="3.00E-99"
                     /label="FHS34_RS41785_AMP-binding.1"
                     /locus_tag="FHS34_RS41785"
                     /protein_end="500"
                     /protein_start="114"
                     /score="324.3"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="ENMAARMPDAIAVTSADGELTYRQLDERADHIAHALLAQGVHLES
                     VVAVALPRSPELITAILGILKAGAAYLPIDPAYPADRVESVLSDASPAVVVTDSATAGS
                     VVGNGLRHLLVDEVTAAGAHPRPALLPDNLAYLMYTSGSSGVPKGVGITHASVTLCAQE
                     LATRFDIKPGGITLASTSVSFDVSVFEIVATLASGGTVDMVQDVLEFARRDTWAGSVVS
                     TVPSAFAELLNDLPESITIDTVVLAGEALPADLVRRLRQALPETRIFNAYGQTESFYAT
                     TTGPLSAMDERSNAPIGTPLGGVHVYVLDPDLRLVPRGVAGELYVAGPNIGRGYHALPG
                     LTAPRFVADPFGVAGARMYRTGDLVRWTRDGELVFVGRADDQVKIR"
     aSModule        343..2022
                     /complete
                     /domains="nrpspksdomains_FHS34_RS41785_AMP-binding.1"
                     /domains="nrpspksdomains_FHS34_RS41785_PCP.1"
                     /locus_tags="FHS34_RS41785"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       526..567
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41785_0001"
                     /evalue="5.90E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="FHS34_RS41785"
                     /protein_end="189"
                     /protein_start="175"
                     /score="19.3"
                     /tool="antismash"
                     /translation="LGILKAGAAYLPID"
     CDS_motif       745..804
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41785_0002"
                     /evalue="1.50E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="FHS34_RS41785"
                     /protein_end="268"
                     /protein_start="248"
                     /score="26.8"
                     /tool="antismash"
                     /translation="AYLMYTSGSSGVPKGVGITH"
     CDS_motif       1291..1380
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41785_0003"
                     /evalue="1.10E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="FHS34_RS41785"
                     /protein_end="460"
                     /protein_start="430"
                     /score="40.2"
                     /tool="antismash"
                     /translation="PRGVAGELYVAGPNIGRGYHALPGLTAPRF"
     CDS_motif       1465..1530
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS41785_0004"
                     /evalue="6.20E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="FHS34_RS41785"
                     /protein_end="510"
                     /protein_start="488"
                     /score="40.5"
                     /tool="antismash"
                     /translation="FVGRADDQVKIRGFRVEPGEVE"
     aSDomain        1816..2022
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS41785_PCP.1"
                     /evalue="2.10E-26"
                     /label="FHS34_RS41785_PCP.1"
                     /locus_tag="FHS34_RS41785"
                     /protein_end="674"
                     /protein_start="605"
                     /score="83.9"
                     /tool="antismash"
                     /translation="EEMLAGLFAEVLEVDQVGIDDGFFALGGHSLLATRLTSRIRSVLG
                     FEVPIRAVFENPTVAGLTPWLTHN"
ORIGIN
        1 ctcccccacc tcaccctcgg caacatcaac accacctacg aacccacctc aacccgcacc
       61 tcacgattcg acctcctctt caacatcgtc gaaccctcgc acgacagcgc ggcccagccg
      121 ggatacgccg gtcacgtcga gtacgccacc gacctgttcg acaaggaaac catcgaacag
      181 ctcatcaccc ggttcaaaac agttctgcgc acggtggtcg ccaccccgga cacaccgctc
      241 cgaaccctcg acgtgctcct ccccgacgag cgccaccgac ttctcgaagc ctggggccgc
      301 acttcgacag ccgcatcgat caccacggtt ccagggctga tcgagaacat ggcggcgcgg
      361 atgccggacg cgatcgcggt cacctccgcc gatggcgaac tgacctaccg gcagctcgac
      421 gaacgcgccg accacatcgc gcatgcgctg ctcgcgcagg gcgtacacct cgaatcagtg
      481 gtggcggtcg cgctcccccg ctcgccggaa ctgatcaccg ccatcctcgg aatcctgaaa
      541 gccggcgccg cctacctgcc catcgacccc gcctacccgg cggaccgggt ggagtccgtg
      601 ctgagcgacg cgtcgccggc ggtcgtcgtc accgacagcg ccacagccgg ttccgtggtc
      661 ggcaacggtc tacgtcatct cctcgttgac gaggtcaccg cggccggtgc gcacccgcga
      721 cccgcgttgc tgccggacaa tctcgcttat ctgatgtaca cctccggttc ctccggtgtc
      781 ccgaaaggcg tggggatcac ccacgcgagc gtcacgctct gcgcgcagga actcgccacc
      841 cggttcgaca taaagccggg aggaatcacg ctggcgagca cctcggtcag tttcgatgtg
      901 tcagtcttcg aaatcgtggc gacgttggcg tctggcggca ccgtcgacat ggtccaggac
      961 gtgctggagt tcgctaggcg cgacacgtgg gccggcagcg tcgtcagcac tgtcccgtcg
     1021 gcgttcgccg agctgctgaa cgacctgccc gaatccatca ccattgacac cgtggtacta
     1081 gccggcgagg cactccccgc tgacttggtc cgtcgcctcc ggcaggcact accggaaacc
     1141 cggatcttca acgcctacgg gcaaacggag tccttctacg ccacgacaac cgggccactg
     1201 tccgcgatgg acgagcggag caacgctccg ataggcaccc cgctcggagg cgtccacgtc
     1261 tacgtcctgg accccgatct ccgtctcgtc ccacggggcg tagccggcga actctacgtt
     1321 gccgggccga acatcggccg cggataccac gctcttcctg gtctcaccgc accgcggttc
     1381 gtcgcggatc cgttcggtgt ggccggtgcg cggatgtacc ggaccgggga tctggtccgg
     1441 tggacgcgcg acggcgagct ggtgttcgtg ggccgcgccg atgaccaggt gaagatccgg
     1501 ggattccggg tcgagcccgg cgaggtcgaa gccgtactcg gctcgcatcc ccacgtgtcg
     1561 cgggccgtgg tggtggcccg cgacggcgtc ggcggcacac aactggtcgc ctacgccgtc
     1621 ggagtcggca gtggcccgcc agacggagag gaattgcgga ggttcctggc ttcccggctg
     1681 ccggagttca tggtgccggc cgcagtcatg gtgctcgacg agttgccgct gacggtgaac
     1741 gggaagctgg accggcgggc actgcccgag ccggagttcc tcggcgacgc ctaccgcgcc
     1801 ccccgcaacc tccacgaaga gatgctggcc ggactgttcg ccgaagtcct ggaggtcgac
     1861 caggtcggca tcgacgacgg cttcttcgca ctcggcggcc actcactgct cgccacccgg
     1921 ctcacctcac gcatcaggtc ggtgctcggc ttcgaggtgc cgatccgcgc ggtcttcgag
     1981 aacccgaccg tagccggact gacaccttgg ctgacccaca accggccggt gcagatcccg
     2041 gtgacc
//
