LOCUS       NZ_JACHJK010000043      2653 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Streptomyces echinatus strain CECT 3313 Ga0436950_43, whole genome
            shotgun sequence.
ACCESSION   NZ_JACHJK010000043
VERSION     NZ_JACHJK010000043.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces echinatus
  ORGANISM  Streptomyces echinatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 05:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,466
            CDSs (total)                      :: 8,381
            Genes (coding)                    :: 8,094
            CDSs (with protein)               :: 8,094
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 5, 1 (5S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 116 of 287
            Pseudo Genes (incomplete)         :: 236 of 287
            Pseudo Genes (internal stop)      :: 12 of 287
            Pseudo Genes (multiple problems)  :: 77 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2653
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJK010000043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..2653
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1830](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <1..1830
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     gene            <1..1830
                     /locus_tag="FHS34_RS43675"
                     /old_locus_tag="FHS34_008488"
     CDS             <1..1830
                     /EC_number="2.1.-.-"
                     /NRPS_PKS="Domain: nMT (92-313). E-value: 2.1e-107. Score:
                     351.0. Matches aSDomain:
                     nrpspksdomains_FHS34_RS43675_nMT.1"
                     /NRPS_PKS="Domain: PCP (522-590). E-value: 9.9e-30. Score:
                     94.6. Matches aSDomain: nrpspksdomains_FHS34_RS43675_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013382.5"
                     /locus_tag="FHS34_RS43675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_008488"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_280528509.1"
                     /sec_met_domain="AMP-binding (E-value: 9.7e-09, bitscore:
                     21.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.6e-19, bitscore:
                     56.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VRGFRIELGEIETALTTHEGVAQAAVVVREDRPGDHRIVAYVVPK
                     TGTGQPDDQVEEEQVEEWQQVYDSIYAESKDAVGFGDDFTGWVSSYDGQPIPLEQMRAW
                     RDATVDRILSLKPQRILELGVGSGLLLAKLAGHCAEYWGSDFSSTVIEKLRSDVERQPD
                     IADRVHLLCQPADATSGLPAEYFDTVVVNSVMQYFPGSAYLLEVVENALRLLKPGGRLF
                     IGDVRNLDLGRTFHAAVHIARSENAEDVAAVRLAVGRAVSEEKELLAAPAFFTALRDRL
                     DDVNAVDIRIKRGEHHNELTRHRYDVIVHKQAPATVSFAHTESLRWGRDVSGTDELARY
                     VGQHRPAALRITGVANRRLAHEVALLQAIDADESIAEVRRLAADRNGTGLDPETFHALG
                     AEWGYQVVTTWSDTGSEGSFDVVLHHAQDDASVLTDVYLPGAGRRAADGTGLTNDPAGQ
                     RQTAALIASLRRELPEVLPEHMVPSAVVSLDRLPLSPNGKLDRRALPAPDFTGRVTGRG
                     PRTPQEETLCDLFAEVLGLPRVGIDDSFFELGGHSLLATRLISRVRVALGVEVSIGSIF
                     DAPTVAGLAGHLKNVEKTQRPKLRRMQRPGETR"
     source          1..2653
                     /db_xref="taxon:67293"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces echinatus"
                     /strain="CECT 3313"
                     /submitter_seqid="Ga0436950_43"
                     /type_material="type strain of Streptomyces echinatus"
     region          1..2653
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2653
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       4..39
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS43675_0001"
                     /evalue="1.30E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="FHS34_RS43675"
                     /protein_end="12"
                     /protein_start="0"
                     /score="14.7"
                     /tool="antismash"
                     /translation="VRGFRIELGEIE"
     aSDomain        280..942
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS43675_nMT.1"
                     /domain_subtypes="nMT"
                     /evalue="2.10E-107"
                     /label="FHS34_RS43675_nMT.1"
                     /locus_tag="FHS34_RS43675"
                     /protein_end="313"
                     /protein_start="92"
                     /score="351.0"
                     /tool="antismash"
                     /translation="GQPIPLEQMRAWRDATVDRILSLKPQRILELGVGSGLLLAKLAGH
                     CAEYWGSDFSSTVIEKLRSDVERQPDIADRVHLLCQPADATSGLPAEYFDTVVVNSVMQ
                     YFPGSAYLLEVVENALRLLKPGGRLFIGDVRNLDLGRTFHAAVHIARSENAEDVAAVRL
                     AVGRAVSEEKELLAAPAFFTALRDRLDDVNAVDIRIKRGEHHNELTRHRYDVIVHKQA"
     aSModule        280..1773
                     /domains="nrpspksdomains_FHS34_RS43675_nMT.1"
                     /domains="nrpspksdomains_FHS34_RS43675_PCP.1"
                     /incomplete
                     /locus_tags="FHS34_RS43675"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        1570..1773
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS43675_PCP.1"
                     /evalue="9.90E-30"
                     /label="FHS34_RS43675_PCP.1"
                     /locus_tag="FHS34_RS43675"
                     /protein_end="590"
                     /protein_start="522"
                     /score="94.6"
                     /tool="antismash"
                     /translation="EETLCDLFAEVLGLPRVGIDDSFFELGGHSLLATRLISRVRVALG
                     VEVSIGSIFDAPTVAGLAGHLKN"
     gene            1857..>2653
                     /locus_tag="FHS34_RS43680"
                     /old_locus_tag="FHS34_008489"
     CDS             1857..>2653
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (0-266). E-value:
                     2e-95. Score: 311.2. Matches aSDomain:
                     nrpspksdomains_FHS34_RS43680_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019985304.1"
                     /locus_tag="FHS34_RS43680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_008489"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_280528510.1"
                     /sec_met_domain="Condensation (E-value: 2e-56, bitscore:
                     178.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWFLHRLEGVSATYNIPLAMRLSGNLDRAALRLALTDVVERHESL
                     RTVFPEVDGVPRQKVLSVSEAGVGLSVVPTTEEELAAGLADASAEGFDLANDLPLRVTL
                     FVLSPTEHVLLLVLNHIAADGWSFAPLSRDVGEAYAARAKGQSPNWPELPVQYVDYTLW
                     QQELLGDENDPESLISRQAAYWEKALAGIPEQLELPADRPRPAVAGYAGAAVPLTIDPD
                     LHGRIVALAHECGASVFMVLQAGLAVLLKRLGAGSDIPLGSPIA"
     aSDomain        1857..>2653
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS43680_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.00E-95"
                     /label="FHS34_RS43680_Condensation_LCL.1"
                     /locus_tag="FHS34_RS43680"
                     /protein_end="266"
                     /protein_start="0"
                     /score="311.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: NHIAADG"
                     /tool="antismash"
                     /translation="MWFLHRLEGVSATYNIPLAMRLSGNLDRAALRLALTDVVERHESL
                     RTVFPEVDGVPRQKVLSVSEAGVGLSVVPTTEEELAAGLADASAEGFDLANDLPLRVTL
                     FVLSPTEHVLLLVLNHIAADGWSFAPLSRDVGEAYAARAKGQSPNWPELPVQYVDYTLW
                     QQELLGDENDPESLISRQAAYWEKALAGIPEQLELPADRPRPAVAGYAGAAVPLTIDPD
                     LHGRIVALAHECGASVFMVLQAGLAVLLKRLGAGSDIPLGSPIA"
     CDS_motif       1890..2006
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS43680_0001"
                     /evalue="2.20E-20"
                     /label="C2_LCL_024-062"
                     /locus_tag="FHS34_RS43680"
                     /protein_end="50"
                     /protein_start="11"
                     /score="65.0"
                     /tool="antismash"
                     /translation="ATYNIPLAMRLSGNLDRAALRLALTDVVERHESLRTVFP"
     CDS_motif       2214..2273
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS43680_0002"
                     /evalue="7.80E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="FHS34_RS43680"
                     /protein_end="139"
                     /protein_start="119"
                     /score="24.9"
                     /tool="antismash"
                     /translation="HIAADGWSFAPLSRDVGEAY"
     CDS_motif       2322..2360
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS43680_0003"
                     /evalue="8.60E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="FHS34_RS43680"
                     /protein_end="168"
                     /protein_start="155"
                     /score="15.1"
                     /tool="antismash"
                     /translation="QYVDYTLWQQELL"
ORIGIN
        1 gtccgcggct tccgcatcga actcggcgaa atcgaaacag ccctcaccac ccacgagggc
       61 gtggcccagg ccgcggtcgt ggtacgcgaa gaccgccctg gcgaccaccg catcgtcgcc
      121 tacgtcgtac cgaaaacggg tacggggcag ccggacgacc aggtggaaga ggagcaggtc
      181 gaggaatggc agcaggtcta cgactccatc tacgcggagt ccaaggacgc cgtcggcttc
      241 ggtgacgact tcacggggtg ggtcagcagt tacgacggac agcccattcc cctggagcag
      301 atgcgtgcct ggcgtgacgc caccgtcgac cgcatcctgt ccctgaagcc gcagcgcatt
      361 ctggaactgg gcgtgggaag cggcctgttg ctggcgaagc ttgccggcca ctgcgcggag
      421 tactggggaa gcgacttctc gtccaccgtc atcgagaagc tgcggtccga cgtcgagcgg
      481 cagccggaca tcgcggaccg cgtgcacttg ctgtgccagc ccgccgacgc gacgagcgga
      541 ctgccggcgg agtacttcga cacggtcgtc gtcaactccg tgatgcagta cttccccggc
      601 agcgcctatc tcctcgaggt ggtggagaac gcactgcgtc tgctcaagcc cggcggccgg
      661 ctgttcatcg gcgatgtacg caacctggac ctgggccgca ccttccatgc cgccgtgcac
      721 atcgcgcgtt cggagaacgc cgaggacgtc gcggccgtgc gcctcgccgt cggccgggcg
      781 gtgtcggagg agaaggaact cctcgcggcc ccggccttct tcacggccct gcgcgaccgg
      841 ctcgacgacg tcaacgcggt ggacatccgc atcaagcggg gcgagcacca taatgagctg
      901 acccggcatc gttacgacgt catcgtgcac aagcaggcgc cggcgaccgt ttccttcgcg
      961 cacaccgagt cgctgcgctg gggccgggac gtctccggca cggacgagct ggcccggtac
     1021 gtggggcagc atcgacccgc cgccctgcgt atcaccggtg tcgccaaccg acggctggcg
     1081 cacgaggtcg ccctgttgca ggcgatcgac gcggacgagt cgatcgcgga ggtgcgccgg
     1141 ctcgccgccg accgcaacgg cacgggcctg gaccccgaga cgttccacgc cctcggcgcg
     1201 gaatggggct accaggtggt gacgacctgg tcggacaccg gctccgaggg gtcgttcgac
     1261 gtggtcctcc accatgccca ggacgacgcc tccgtgctca ccgatgtcta tctgcccggc
     1321 gccgggcgcc gggcagcgga cggtaccggc ctgacgaacg accccgcggg tcagcgtcag
     1381 acggcggcgc tcatcgcctc gctgcgccgg gagctgccgg aagtactgcc cgagcacatg
     1441 gtgccgtccg cggtggtgag cctggaccgc ctgccgttgt cgccgaacgg aaagctcgac
     1501 cgcagggccc tgcccgcgcc ggacttcacc ggacgcgtca ccgggcgcgg cccgcgcacg
     1561 ccacaggaag agaccctgtg cgacctgttc gccgaagtac tcggactgcc tcgcgtcgga
     1621 atcgatgaca gtttcttcga gctgggcggt cactccctgc tcgcgacgcg cctgatcagc
     1681 cgggtgcggg tcgcgctcgg cgtggaggtc tcgatcggca gcatcttcga tgccccgacc
     1741 gtcgccgggc tggccggcca cctgaagaac gttgaaaaga cacagaggcc gaagctgcgt
     1801 cgcatgcaga ggccggggga gactcgatga ttccgctctc gtacgcgcaa cgccggatgt
     1861 ggttcctgca ccggctcgaa ggggtcagcg cgacctacaa cattcccttg gcgatgcgcc
     1921 tctcgggaaa cctggaccgg gcagccctgc ggctggcact caccgacgtc gtcgagcgtc
     1981 atgagagcct gcggacggtc ttccccgagg tcgacggggt gccgcggcag aaggtgctga
     2041 gcgtctccga ggccggggtc gggttgtcgg tggtgccgac gacggaggag gagctcgcgg
     2101 cggggctcgc cgacgcgtcc gccgaaggct tcgatctggc gaacgacctc ccgttgcggg
     2161 tgacgctgtt cgtgctgtcg ccgaccgagc acgtgctgtt gctggtgctc aatcacatcg
     2221 ccgccgacgg ctggtccttc gcgcccctgt cgcgggacgt gggcgaggcg tacgcggctc
     2281 gggcgaaggg tcagtcgccc aactggccgg agcttccggt gcagtacgtc gactacacgc
     2341 tgtggcagca ggagctgctg ggggacgaga acgaccccga gagcctgatc agtcgccaag
     2401 cggcctattg ggaaaaggcg ttggcgggca ttccggagca gctggagctt cccgcggacc
     2461 gtccgcggcc ggcggtggcc ggttatgccg gtgccgccgt cccgctcacg atcgaccccg
     2521 acctgcacgg gcggatcgtg gcgctggcgc acgagtgcgg ggcgagtgtg ttcatggtgc
     2581 tgcaggccgg tctcgcggtg ctgctcaagc gcctgggcgc gggatccgac atcccgctgg
     2641 gctcacccat cgc
//
