LOCUS       NZ_JACHJK010000025     11929 bp    DNA     linear   CON 29-SEP-2024
DEFINITION  Streptomyces echinatus strain CECT 3313 Ga0436950_25, whole genome
            shotgun sequence.
ACCESSION   NZ_JACHJK010000025
VERSION     NZ_JACHJK010000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces echinatus
  ORGANISM  Streptomyces echinatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 59990)
  AUTHORS   Whitman,W.
  TITLE     Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the
            genomes of soil and plant-associated and newly described type
            strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 59990)
  AUTHORS   Whitman,W., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 160.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014203595.1-RS_2024_09_29
            Annotation Date                   :: 09/29/2024 05:39:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,466
            CDSs (total)                      :: 8,381
            Genes (coding)                    :: 8,094
            CDSs (with protein)               :: 8,094
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 5, 1 (5S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 116 of 287
            Pseudo Genes (incomplete)         :: 236 of 287
            Pseudo Genes (internal stop)      :: 12 of 287
            Pseudo Genes (multiple problems)  :: 77 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:56:37
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 25144
            Orig. end                         :: 37073
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHJK010000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11929
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11929
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11929
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6929](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6929
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            650..1366
                     /locus_tag="FHS34_RS39075"
                     /old_locus_tag="FHS34_007926"
     CDS             650..1366
                     /NRPS_PKS="Domain: PKS_KR (13-100). E-value: 5.1e-12.
                     Score: 38.0. Matches aSDomain:
                     nrpspksdomains_FHS34_RS39075_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012334.5"
                     /locus_tag="FHS34_RS39075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007926"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_245002582.1"
                     /transl_table=11
                     /translation="MTSPTAEHARTISLVTGGNRGIGREVCRQLAALGHTVLLTSRSPA
                     SAEAAARQLARDGSSVHPLQLDVRDPDSVGAAAREVAKDFGRLDMLVNNAAIMYDTWQR
                     APPPISPPSPRPPRPVSTAPGAPHRPSCLLRQSPHARIVNVSSEAASLAGLGGGTPAYT
                     ITKAGLNALTLMLAAELRGDRILVNAVCPGRVATDMGGPGGRPVPEGAAGIVWAATLPH
                     DGPTGGFYRDGLPIPW"
     CDS_motif       689..817
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FHS34_RS39075_0001"
                     /evalue="6.80E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="FHS34_RS39075"
                     /protein_end="56"
                     /protein_start="13"
                     /score="25.2"
                     /tool="antismash"
                     /translation="LVTGGNRGIGREVCRQLAALGHTVLLTSRSPASAEAAARQLAR"
     aSDomain        689..949
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS39075_PKS_KR.1"
                     /evalue="5.10E-12"
                     /label="FHS34_RS39075_PKS_KR.1"
                     /locus_tag="FHS34_RS39075"
                     /protein_end="100"
                     /protein_start="13"
                     /score="38.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="LVTGGNRGIGREVCRQLAALGHTVLLTSRSPASAEAAARQLARDG
                     SSVHPLQLDVRDPDSVGAAAREVAKDFGRLDMLVNNAAIMYD"
     misc_feature    1618..1632
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.18"
                     /tool="antismash"
     misc_feature    complement(1726..1740)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.38"
                     /tool="antismash"
     gene            1767..3008
                     /locus_tag="FHS34_RS39080"
                     /old_locus_tag="FHS34_007927"
     CDS             1767..3008
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569708.1"
                     /locus_tag="FHS34_RS39080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007927"
                     /product="MFS transporter"
                     /protein_id="WP_184974758.1"
                     /transl_table=11
                     /translation="MERPVSPARPSHQRAESADFRLLWWAGAADGIGSQASGTVLPLLL
                     LGLGNSPQTVGLVAGASTALGLLLGPFVALPADRGARKRVMIGSAAVSALAMGCVVLTL
                     STARPPLGLILAAVLVERVATACFEAAARGTVALVCPGPEQPRAIARLTAADQGALVAG
                     PALGGLLYQAARALPFLADALSYVVSALCVTAMRADLTAPAPRQPGEGVLRESAAGLRL
                     VRSSPLLRLVLVWGTTVNAVVVALYYGAVFTLQRDGRSGAVLGVILALSGAAGLVGALT
                     APNVAKRFGASRVVTGVTWLLLPLAVALAAVSDAWAYGLLFSLVCLLMPLASVVLQARA
                     LQATAPALQARTGAVLATATTGAAALAPVLAGALADRVSPAAPAVFCGVLLTLLAAHSS
                     RRPVRGVLRAEVSG"
     gene            3005..4858
                     /locus_tag="FHS34_RS39085"
                     /old_locus_tag="FHS34_007928"
     CDS             3005..4858
                     /NRPS_PKS="Domain: Abhydrolase_1 (349-508). E-value: 1e-09.
                     Score: 30.3. Matches aSDomain:
                     nrpspksdomains_FHS34_RS39085_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129145.1"
                     /locus_tag="FHS34_RS39085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007928"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_184974761.1"
                     /sec_met_domain="PF00561 (E-value: 5.6e-09, bitscore: 28.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 4.8e-39, bitscore:
                     126.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDGYQVYRAALPDVCAADPARMVFTADAEGRCEVFTWDAGTRRA
                     RQVTRRPLGTLHGAIDRDAYVWWFDEDEHGAGSWRFQPFTGGPDRPGLTNVPPGLPRGL
                     AVSDHGTVAVAVAAGDGDATTVHVGPRGGAGRAVARIPGHVTLTGITPGGRLLALAGAG
                     TVTVLTHEGAVRAVLPGAGDPGRLWSLGFAPATDDAELLLVREDGDRYVLASWRPATGP
                     VTHDWCAFDTEITARWESDGRSVLVRQDRHGRSTLHRADLAAHTLTPVPTPPGTVLDAA
                     TRPGGDLHYLWTAAGDPPRLCSTRGTALPVLPTLPDRFPGTHQDVRTPGPDGPVHTLLS
                     LPDAPRGPVPAVFLVHGGPADHDRDAYDGIVHSLVASGFAVARVNYRGSTGYGPRWRRA
                     FRDGVGLTQVEDLASVRADLVARGLIRADAVGLWGTSWGGYLVLLALGTRPGLWQAGVA
                     VKPVADCVAAHRTTTPALRALDERLFGGTPEEVPERYARSSPIHYAAAVRAPLLVAAAT
                     HDAKCPPGQIHGYLAALRAAGVRHEALWLDSGHDGYDGGDHVTVLRRAVVFLDRELRAP
                     ARRHPQTPRSDRSGEHHTAVHTTERRRTHAEGRHSQRPARG"
     aSDomain        4052..4528
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FHS34_RS39085_Abhydrolase_1.1"
                     /evalue="1.00E-09"
                     /label="FHS34_RS39085_Abhydrolase_1.1"
                     /locus_tag="FHS34_RS39085"
                     /protein_end="508"
                     /protein_start="349"
                     /score="30.3"
                     /tool="antismash"
                     /translation="PAVFLVHGGPADHDRDAYDGIVHSLVASGFAVARVNYRGSTGYGP
                     RWRRAFRDGVGLTQVEDLASVRADLVARGLIRADAVGLWGTSWGGYLVLLALGTRPGLW
                     QAGVAVKPVADCVAAHRTTTPALRALDERLFGGTPEEVPERYARSSPIHYAAAVR"
     gene            5001..6929
                     /locus_tag="FHS34_RS39090"
                     /old_locus_tag="FHS34_007930"
     CDS             5001..6929
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958123.1"
                     /locus_tag="FHS34_RS39090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007930"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_184974764.1"
                     /sec_met_domain="TIGR03975 (E-value: 1.2e-190, bitscore:
                     626.9, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPGAEAEVLHANLEF
                     TDWITARAEFTGDDYEYYSLSSYFMGCGDWVFSSALYDDPHWREEEFTAVMRGKLKKSR
                     MAMTHALHQVVPEFVDEIARRIVAHAPDVVGFTSTFQQNAAALAAARRVKQLAPHIVTV
                     MGGANCDGAQGAAVHRNFPFVDHVVRGEGEAAFPQLLTALTEGSDLAEVPGLCHRGPDG
                     ESVANAMATRPLPPAAILPPDYSGYFERLAASAARHWVEPKLVVEGARGCWWGEKHHCT
                     FCGLNGSFMEFRSKSPDTFYEEIIELARRHRVLDMYVVDNILDMGYLTTVLPRIIDSEY
                     DLRLHIEIKANMRRQQLRTLADAGMIYVQPGIESLNHRVLDLMDKGVSGCQNVRMLRDG
                     AETGLSVAWNYLHGFPGETAQDYDPVIAQIPALEHLDPPVDLSARIAIERFSPYFERPE
                     LGFTGLRPEEHYRFTYDLPEEELYDLAYVFEAPARGIGEPTVTALNDALTAWKKHHADA
                     RLTHTDLGDRIVLVSRRRAFEWRTTELADPFELVAFRLLDQPHSVAALTRKAAGKAPGA
                     ARDEAAVQRLLDRWVSLGIVFTDGGQYVHIAPAAVNEDLLRLDFMRHTHGARSAPAEPA
                     PAEPVHA"
     gene            6955..7623
                     /locus_tag="FHS34_RS39095"
                     /old_locus_tag="FHS34_007931"
     CDS             6955..7623
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129142.1"
                     /locus_tag="FHS34_RS39095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007931"
                     /product="DUF5825 family protein"
                     /protein_id="WP_184974767.1"
                     /transl_table=11
                     /translation="MSTSSTMSPPSVLAWRDYDPSACELPGMFLGELALPGPLEGESER
                     LWQLGARRVRLDAIVDLTPGAGAERAAAAVAALSLVRDLTARAVLVEWDVRLPPAPEEA
                     WQVLSHLQPPRRIEGPSGADEALRAWQQGHYLCKCLWRKGPGFVQIRDRRWGELRRFTA
                     DEPHYEDTIERLAYGAPAASVPAAVLDDFRGEKLVLDIGPLAWWLPYRISRWLQEPMTI
                     "
     gene            complement(7731..8021)
                     /locus_tag="FHS34_RS39100"
                     /old_locus_tag="FHS34_007932"
     CDS             complement(7731..8021)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670498.1"
                     /locus_tag="FHS34_RS39100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007932"
                     /product="DUF6355 family natural product biosynthesis
                     protein"
                     /protein_id="WP_184974770.1"
                     /transl_table=11
                     /translation="MYVRRLLTSALGTVSLALGLLGAGAATATPAAADPCGFYETGSDA
                     YYNHCTSDGSHVVIKVEVALAPDYERCVGPGRSWLGSASSIQGAYYVGRTC"
     misc_feature    8117..8131
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            8362..8682
                     /locus_tag="FHS34_RS39105"
                     /old_locus_tag="FHS34_007933"
     CDS             8362..8682
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FHS34_RS39105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FHS34_007933"
                     /product="hypothetical protein"
                     /protein_id="WP_184974773.1"
                     /transl_table=11
                     /translation="MRAQHVRRVLAASAAGVVMAGGAAVGAAGTAVAAAPAAHTGRVTT
                     GGCFGHDDWRCFDDGGFGHGGFWHGGFDRDGFRHGGFGHGGFGHHFDHRFDRGPFVIIV
                     VR"
     gene            complement(8839..9249)
                     /locus_tag="FHS34_RS39110"
                     /old_locus_tag="FHS34_007934"
     CDS             complement(8839..9249)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FHS34_RS39110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FHS34_007934"
                     /product="hypothetical protein"
                     /protein_id="WP_184974831.1"
                     /transl_table=11
                     /translation="MRVFVLCAWLLTAGLGGYLAVVWIRHGGLRQRTPGVTRLPLWLVV
                     GHVALAVAGLAAWSAYLLGGVRSSAWAACAVVLIVAAFGFMMLLRWLPSSGRHAHGAAT
                     AERHFPLAAVVAHGTSAGATVLLVLLVVLRRV"
     misc_feature    complement(9582..9596)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.32"
                     /tool="antismash"
     gene            9589..10134
                     /locus_tag="FHS34_RS39115"
                     /old_locus_tag="FHS34_007935"
     CDS             9589..10134
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004978969.1"
                     /locus_tag="FHS34_RS39115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007935"
                     /product="DoxX family protein"
                     /protein_id="WP_184974776.1"
                     /transl_table=11
                     /translation="MATHNTGRSSTFLRQPVPEQRVTAGDCGLLLLRLTFGLFMAGHGA
                     QKLFGLFGGQGLTATGKGFDALGYHPGKVFAVIGGLSEFLGGLGLAVGLFTPLAAAALI
                     GVMINAMVTVTGAHGLWDTDGGVEYNVAIAIVALAVAAIGPGRLAIDRFFRWGDGGWTA
                     AAVALGLGGIASAISLTL"
     gene            complement(10618..10875)
                     /locus_tag="FHS34_RS43115"
                     /old_locus_tag="FHS34_007936"
     CDS             complement(10618..10875)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024734.5"
                     /locus_tag="FHS34_RS43115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FHS34_007936"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_225817885.1"
                     /transl_table=11
                     /translation="MFELIRRERRPVTPEEVAEGLGISRKPAAFHLDKLVAAGLLQAHY
                     EAPTGIRKVGRRPKVYEPVDETIAVHIPERRYGMPADVLT"
ORIGIN
        1 gggttcccag ccggcccgga tcagcaggcc gagcccgaac tcccgtcccc gcagggcgtt
       61 gggcccgagc aactgcccca tggtcagcaa cggcacgact gcgggatgcg cgagcaggac
      121 ccgccggaac tccccggccg cctgttccat gtccgcccgc cacccgcggc cggtcgcccc
      181 tggcaccacc tcgcccagca catggtcgac gagttccacc agcaactcgt ccctgctggc
      241 cacatgccgg tacagcgagg tgtgccgtac tcccatgtcc tcggccaccc tgcgcatggt
      301 gagcgcggcg agcccttcgg cgtccacgat gtccagcgcc gctcgggtga tccgcttcag
      361 ttcgagcccg cccgggcgcg gtcgctgacg cccgcgtgcc gcgtaccgcg cccgccacca
      421 ttccgccgat cccgccttca ccccgcaact atgcgtccag tggacgcata acgcaagagt
      481 gcgctccgac gacggcgggc ggactgcggc ccgggcgcgc cccacggcct gccccggctc
      541 aggcggccgc cgatcttgac gcgtccctca tggtgaaagc ttcgacaact cctcttctcc
      601 cgccgcagca tcgaaagcac cacggtgtac gcagacagga gccctcccga tgacctcccc
      661 cacggccgag cacgcccgca cgatctccct cgtcaccggg ggcaaccgtg gcatcggacg
      721 tgaggtgtgc cgccagctcg ccgccctcgg gcacaccgtg ctgctgacct cgcgctcgcc
      781 ggcgtcggcc gaggcggcgg cccggcagct cgcccgggac ggctcgtccg tgcacccgct
      841 gcagctggac gtccgcgacc ccgacagcgt cggcgccgcc gcccgcgagg tggcgaagga
      901 cttcggacgc ctggatatgc tcgtcaacaa cgccgcgatc atgtacgaca cctggcagcg
      961 cgccccaccg ccgatctcgc caccgtcacc gaggccgccg aggccagtct ctacggcccc
     1021 tggcgcacca cacaggcctt cctgcctgct ccggcagagc ccgcacgcca ggatcgtcaa
     1081 tgtcagcagc gaggcggcgt ccctggccgg cctgggcggc ggcacacccg cctacaccat
     1141 caccaaggcc gggctcaacg ccctcaccct catgctcgcg gccgaactcc ggggcgaccg
     1201 catactcgtc aacgccgtct gccccggccg ggtcgccacc gacatgggcg gaccgggggg
     1261 ccgtcccgtc ccggagggcg ccgccggcat cgtatgggcc gccacactcc cccacgacgg
     1321 ccccaccggg ggcttctacc gcgacggcct ccccatcccc tggtgacacc ccgcgggtgc
     1381 gccgggtccg gccccccggt cggacagcgg cacacccgcc gcgccgcgtg ccgcacggcg
     1441 gcgactgttc tcggtcgaaa gcggcgcctc gccgatgcac ccgaagggtt cgcacccgcc
     1501 gcccggacct ccggaccgat ccggcgaggc gcagaccctg acacgcattc ccgctggagg
     1561 aataggggct ccattccgcc ctcgcacgga atgatcacct ctatttgcgt gtatgacgga
     1621 cctttccttc cgcaccccct cacgacatga atccggccat gcgcttccgg ctcttgtgac
     1681 gcctgttcac gcccgcctag gatcccgctc gcttcgcttc cgccacactt tctgcacagt
     1741 ttcgccatac cggtgaggtg ctttccgtgg aacggccagt gtcacccgca cgaccgtccc
     1801 accaacgggc ggaatcagcc gacttcagac tcctgtggtg ggccggcgcg gccgacggga
     1861 tcggcagcca ggcctccggt accgtcctgc cgctgctgct cctgggactg ggcaactcgc
     1921 cccagaccgt gggactggtc gccggtgcct ccaccgccct cgggctcctc ctcggaccgt
     1981 tcgtcgccct gcccgccgac cggggcgccc gcaagcgcgt gatgatcggg tccgcggccg
     2041 tctccgccct cgccatgggc tgcgtggtgc tcaccctgag caccgcaagg cctcccctgg
     2101 gcctgatcct cgccgccgtg ctggtcgagc gggtggcgac cgcctgcttc gaggcggcgg
     2161 cacgcggaac ggtcgcgctg gtctgcccgg gccccgaaca gccccgcgcc atcgcccggc
     2221 tcaccgccgc cgaccagggt gcgctggtcg ccggcccggc cctgggcggg ctcctctacc
     2281 aggccgcccg cgcgctgccg ttcctcgccg acgccctctc ctacgtcgtc agcgcgctgt
     2341 gcgtcaccgc catgcgcgcg gacctcacgg cgccggcacc gcggcagccc ggcgagggag
     2401 tgctgcggga gagcgcggcc gggctgcggc tggtgcggtc ctccccgctg ctgcggctgg
     2461 tgctggtgtg gggcaccacc gtcaacgcgg tcgtggtggc gctgtactac ggagccgtct
     2521 tcaccctcca gcgggacggc cgctccggcg ccgtgctggg cgtgatcctc gccctctcgg
     2581 gcgccgccgg tctggtgggc gcgctcaccg ccccgaacgt ggccaaacgt ttcggcgcct
     2641 cacgggtcgt caccggtgtg acctggctgc tcctcccgct cgccgtcgca ctcgccgccg
     2701 tctcggacgc atgggcgtac ggactgctgt tcagcctggt gtgcctgctg atgccgctcg
     2761 cctccgtcgt gctccaggcc cgtgccctgc aggccaccgc tcccgcgctg caggcccgta
     2821 ccggcgccgt cctggccacc gcgacgaccg gtgcggcggc actggctccc gtcctggcgg
     2881 gcgccctcgc cgaccgggtc tcccccgccg cgcccgcggt cttctgcggt gtgctgctga
     2941 ccctgctcgc cgcccacagc tcccggcgcc cggtgcgtgg tgtcctgagg gcggaggtct
     3001 ccggatgagc gacggctacc aggtgtaccg ggccgccctg ccggacgtgt gcgcggccga
     3061 ccccgccagg atggtcttca ccgcggacgc cgagggccgg tgcgaggtgt tcacctggga
     3121 cgccggcacc cgccgcgccc ggcaggtcac ccggcgcccg ctgggcaccc tgcacggcgc
     3181 catcgaccgg gacgcgtacg tctggtggtt cgacgaggac gagcacgggg cgggcagctg
     3241 gcggttccag cccttcaccg gcggccccga ccggcccggc ctgacgaacg tcccgcccgg
     3301 cctcccccgg ggtctggccg tgagcgacca cggcaccgtc gcggtcgccg tcgcggccgg
     3361 ggacggcgac gccaccaccg tgcacgtggg cccgcgtggc ggcgcgggcc gcgcggtggc
     3421 caggatcccg ggccacgtca ccctcaccgg catcacgccc ggaggccggc tgctcgccct
     3481 tgccggagcg ggaaccgtca ccgttctcac ccacgagggt gccgtacggg ccgtcctgcc
     3541 cggcgccgga gacccgggcc ggctgtggtc cctgggcttc gcccccgcaa cggacgacgc
     3601 ggaactcctt ctcgtccgcg aggacggcga ccggtacgtc ctggcgtcct ggcggccggc
     3661 gaccgggccg gtcacccacg actggtgcgc cttcgacacg gagatcacgg cccgctggga
     3721 gtcggacggc cggtccgtcc tggtccgcca ggaccgccac ggccgctcca ccctgcaccg
     3781 cgccgacctc gccgcgcaca cgctcacccc cgtccccacc ccgccgggaa ccgtgctgga
     3841 cgccgccacc cggcccgggg gcgacctgca ctacctctgg acggccgccg gcgacccgcc
     3901 ccggctgtgt tccacccggg gcaccgccct gcccgtgctg cccaccctgc ccgaccggtt
     3961 ccccggcacc caccaggacg tgcggacacc ggggcccgac ggccccgtgc acaccctcct
     4021 cagcctgccc gacgcaccac gcggcccggt tccggcggtg ttcctggtgc acggcgggcc
     4081 cgccgaccac gaccgggacg cctacgacgg catcgtccac tccctcgtcg cctccggctt
     4141 cgccgtggcc cgcgtcaact accgcggctc caccgggtac gggccgcgct ggcgccgcgc
     4201 cttccgggac ggggtcgggc tcacccaggt cgaggacctg gcgtcggtcc gggccgacct
     4261 ggtcgcccgc gggctgatcc gcgcggacgc cgtgggcctg tggggcacct cctggggcgg
     4321 ctacctggtc ctgctcgccc tcggcacccg tcccggcctg tggcaggcgg gggtcgcggt
     4381 caaaccggtc gccgactgtg tggccgccca ccgcaccacc actccggcgc tgcgcgccct
     4441 ggacgaacgg ctcttcggcg gcacgcccga ggaggtcccc gagcggtacg cgcgcagttc
     4501 gccgatccac tacgccgccg cggtccgcgc cccgctgctg gtggcggcgg cgacgcacga
     4561 cgccaaatgc ccgcccggtc agatccacgg ctacctcgcc gcgctgcgcg ccgccggtgt
     4621 ccggcacgag gcgctgtggc tcgactccgg ccacgacggc tacgacggcg gtgaccacgt
     4681 caccgtgctg cgccgggccg tggtcttcct cgaccgggaa ctgcgcgccc ccgcccggcg
     4741 ccacccacag acgccccgct ccgaccggag cggggagcac cacaccgccg tacacaccac
     4801 ggagaggaga aggacccatg cagaaggacg tcattcacaa cgacccgctc gcgggtgacg
     4861 aggagaaccg caagccgagc gtcggcatca ccgtcaccgt cccgttccgc aacgccgagg
     4921 acgcggaaga ggactgaccc ccgccggccg gcccggtacc actccgggcc ggccggtacc
     4981 cgattttccg aggagccgac atgcgtgtcc tgctggtcaa catgccctgg tcgcccatcg
     5041 accttccgtc cctcgccctg ggaatcctca aacggagcgt ggacgagcgc gtgcccggcg
     5101 ccgaggccga ggtcctgcac gccaatctcg aattcacgga ctggatcacc gcacgggccg
     5161 aattcaccgg tgacgactac gagtactact ccctttcctc atatttcatg ggctgcggcg
     5221 actgggtctt ctcctcggcc ctctacgacg acccgcactg gcgcgaggag gagttcaccg
     5281 cggtgatgcg cgggaaactc aagaagtcgc gtatggccat gacccacgcg ctgcaccaag
     5341 tggtcccgga gttcgtcgac gagatcgcgc ggcggatcgt cgcgcacgca cccgacgtcg
     5401 tcggcttcac ctccaccttc cagcagaacg ccgcggcact cgccgccgcg cggcgggtca
     5461 agcagctcgc cccgcacatc gtcaccgtca tgggcggagc caactgcgac ggggcacagg
     5521 gcgccgccgt gcaccgcaac ttccccttcg tcgaccacgt cgtacgagga gagggcgagg
     5581 ccgccttccc gcagttgctg accgccctca ccgagggctc ggacctggcg gaggtgcccg
     5641 gcctgtgcca ccggggccct gacggggaga gcgtcgccaa cgcgatggcc acccgtccgc
     5701 tgccgcccgc cgcgatcctg ccgcccgact acagcggcta cttcgagcgc ctcgccgcct
     5761 ccgccgcccg ccactgggtc gagccgaagc tggtcgtcga gggcgcccgt gggtgctggt
     5821 ggggcgagaa gcaccactgc accttctgcg ggctcaacgg ctcgttcatg gagttccgtt
     5881 ccaagagccc ggacaccttc tacgaggaga tcatcgaact ggctcgccgc caccgggtcc
     5941 tggacatgta cgtcgtcgac aacatcctcg acatgggcta tctgacgacc gtgctgccgc
     6001 ggatcatcga cagcgagtac gacctgcggc tgcacatcga gatcaaggcc aacatgcgcc
     6061 gccagcagct gcgtaccctc gccgacgcgg ggatgatcta cgtccagccc ggcatcgaat
     6121 cgctcaacca ccgggtgctg gacctgatgg acaagggcgt cagcggctgc cagaacgtcc
     6181 ggatgctccg cgacggagcc gagaccggcc tgtccgtcgc ctggaactac ctgcacggct
     6241 tccccggcga gaccgcgcag gactacgacc cggtcatcgc ccagatacct gcgctcgaac
     6301 acctcgaccc gcccgtcgac ctgtcggccc ggatcgccat cgagcgcttc agcccgtact
     6361 tcgagcgccc cgaactcggc ttcaccggcc tgcggcccga ggagcactac cgcttcacct
     6421 acgacctgcc ggaggaggag ctgtacgacc tcgcgtacgt cttcgaggcg ccggcgcggg
     6481 gcatcggcga accgaccgtc accgcgctga acgacgctct gaccgcgtgg aagaaacacc
     6541 acgccgacgc ccggctgacc cacaccgacc tgggcgaccg gatcgtcctg gtctcccggc
     6601 gccgcgcctt cgagtggcgg acgaccgagc tggccgaccc tttcgaactg gtcgccttcc
     6661 gcctcctcga ccagccgcac agcgtcgccg ccctcacccg caaggccgcg ggcaaggccc
     6721 ccggagccgc ccgtgacgag gccgccgtgc agcggctcct cgaccggtgg gtgtcgctcg
     6781 ggatcgtctt caccgacggc gggcagtacg tgcacatcgc cccggccgcc gtcaacgagg
     6841 acctgctgcg gctcgacttc atgcgccaca cccatggggc ccggtccgcc cccgccgaac
     6901 ccgcccccgc cgaacccgtc cacgcgtgac cgcgccccgc ggaggagtac gcccatgagc
     6961 acgtcgagca ccatgagccc gcccagcgtc ctcgcctggc gcgactacga cccctccgcc
     7021 tgcgaactcc ccggcatgtt cctgggcgaa ctcgcccttc ccggcccgct ggagggcgag
     7081 agcgaacggc tgtggcagct cggggcacgg cgcgtacggc tggacgcgat cgtcgacctc
     7141 acccccggcg cgggcgccga gcgggccgcc gcggcggtgg ccgcgctgag cctggtgcgc
     7201 gacctgaccg cgcgagccgt cctcgtcgag tgggacgtgc ggcttccccc ggcgccggag
     7261 gaagcctggc aggtcctcag ccacctccag ccgccccggc gcatcgaggg accctccggg
     7321 gcagacgagg cactgcgcgc ctggcagcag ggccactatc tgtgcaagtg cctgtggcgc
     7381 aagggacccg gtttcgtgca gatccgggac cgccgctggg gcgagctgcg ccggttcacc
     7441 gccgacgagc cgcactacga ggacacgatc gagcggctgg cgtacggagc gccggcggcc
     7501 tccgtgccgg cggcggtcct cgacgacttc cgcggcgaga agctcgtcct cgacatcggc
     7561 ccgctcgcct ggtggctgcc gtaccggatc agccgctggc tccaggagcc gatgaccatc
     7621 tgacggccga agccgcgccg aattgacggc ggtgacggtc aaggccggcc gggggcgccc
     7681 tccgcgcagg gggctgcccc ggccgggccg ccggagggag tcgccgtccg tcagcaggtg
     7741 cggcccacgt aataggcgcc ctggatgctg gaggcggagc cgagccagct ccggcccggg
     7801 cccacacagc gctcgtagtc gggggccagc gccacctcta ccttgatcac cacgtgggaa
     7861 ccgtcggaag tgcagtggtt gtagtaggcg tcggagccgg tctcgtagaa gccgcacgga
     7921 tccgcggccg ccggcgtcgc ggtggcggcg ccggcaccga ggagtcccag cgcgagggag
     7981 acggtaccga gagcactggt cagcaggcgg cgaacgtaca cggcgaactc ctcgtgacag
     8041 gtggcgtgac aggggggaca acggtgtcgc cgggaagcgg atgaccgctc ggccgggcac
     8101 gcgcccatga tcacggatca gcggggtgga tcggccgtcc ggcctccgct ttcacccgcc
     8161 gaatcatttc ctcggtacgc cgaacggatc acctgtctgg acgcgcgccc ccacgtcgcg
     8221 gtagggcggc gccccggcca atccgaaagg gcgcggcggc cgtatcagga gtagggccgc
     8281 ggccatgccg atccccgccg cggcggttcg tctcgtgccg ccaggagtcg cacgaggcgc
     8341 gggaaggaag gtgcgtccat catgcgagca cagcacgtac ggcgtgtcct cgcggcgtcg
     8401 gcggccggtg tggtgatggc cggcggcgca gcggtcggcg cggcaggcac ggccgtcgcc
     8461 gcggcgcccg ccgcgcacac cggccgggtg accacgggcg gctgcttcgg tcacgacgac
     8521 tggcgctgct tcgacgacgg cggcttcggc catggtggct tctggcacgg tggcttcgac
     8581 cgcgatggct tcaggcacgg tggcttcggg cacggtggct tcgggcacca cttcgatcac
     8641 cgattcgacc gggggccgtt cgtgatcatc gtggtccggt aggagtcgcc ccgtacgaca
     8701 ccgggcgcgt ccgtccaggg gcacgcccgg tgtcgtacgg ggcagcggtg tcagctgctg
     8761 gcgcccgcct gacgcagagc aaggggcgtg tcgccccgac gggtgacggc tcggggcgat
     8821 gccccggagg gaccgtcttc acaccctgcg caggacgacg aggaggacca gcagcaccgt
     8881 ggcgcccgcg ctcgtgccgt gcgcgacgac ggcggcgagc gggaagtgcc gttcggcggt
     8941 ggcggcgccg tgcgcatgac ggccggaact gggcagccag cgcagcagca tcatgaagcc
     9001 gaacgcggcc acgatcagga ccaccgcgca ggccgcccag gcggagctcc tgaccccgcc
     9061 cagcagatac gcgctccagg cggcgagccc cgccacggcg agcgccacat ggccgacgac
     9121 cagccacagc ggcagccgcg tcacgcccgg ggtgcgctgc cgtaggccgc cgtgccggat
     9181 ccacacgacg gcgaggtagc cgccgaggcc cgccgtcagc agccaagcgc acagaacgaa
     9241 gacacgcatc cgccttaccc cctgccccgt gtccggccgg ccctgacccg tcagggctgc
     9301 gcgccccagg acaccgcaca gaacaggagc agcccggaca tccatccgct gattcacccg
     9361 taggtgtgaa tggtggggcg aatcgcatgc gcttcggccc gcgctctgtc gtggcggatc
     9421 caccgcccgc ccccgatgtg cgaaccccgt gctgttgcgg aagcatcgga aggggcggta
     9481 tgcgcactcc gcgacgacgc gcgggtgggc acaccgccga ccggtggacc gacgcctttc
     9541 caggcttggg ggaagcaccc gtctcggcgt ccgtaccgga gttctcacat ggcgacgcac
     9601 aacacgggtc ggtcttcgac ctttctgcgg cagccggtcc cggagcagcg ggtgaccgcc
     9661 ggtgactgcg ggctgctgct tctgaggctg accttcggac tgttcatggc cggacatggc
     9721 gcacagaagc tcttcgggct gttcggcggc caagggctga cagccaccgg caagggattc
     9781 gacgccctcg gctaccaccc ggggaaggtc ttcgccgtga tcgggggact ctccgagttc
     9841 ctcggtggcc tgggcctggc cgtcgggctg ttcacgccgc tcgcggccgc cgcgctgatc
     9901 ggcgtgatga tcaatgccat ggtcaccgtc accggtgctc acggcctgtg ggacacggac
     9961 ggcggcgtgg agtacaacgt ggccatcgcc atcgtcgccc tcgccgtcgc cgccatcggc
    10021 ccgggccggc tggcgatcga ccggttcttc cgctggggcg acggcggctg gacggccgcc
    10081 gccgtcgccc tggggctggg cggcatcgcc tccgcgatct ccctgaccct gtgactccga
    10141 agggatgagg gcggatcggc cggggcaacg gcggcagtgg ggcggaagcg cgccccgggg
    10201 ctcaccgctc gccggcggtg ccggcctccg ggtcgtccgc gggtacctgc ttgccaacag
    10261 ctccacgcag cacgagccgg gccggggggc gagcaccgcc gtcgtcctgt cgcttcccag
    10321 cccgtgcagg tagccggcca ggaacgcctg gttgaccgcg cacacccgtt ccggggcccg
    10381 ggccgccatg gggtggaagg ggcagttgcg cagccgcggc agggtgggcc cggtgcgctc
    10441 cggctcgaag ccggactcct ccggggtgtc ggcggccacg ctcagtccgc gctcggcgcc
    10501 gaggcggccg ggacgtagcc gttcgcgttc ggcgccgccc agggtctctc cgcggcggta
    10561 ggccacacgg agggcggcgt cgcgggccga ttccccgtcc gtctcggtga gcacggctta
    10621 ggtgaggacg tcggccggca tgccgtagcg gcgctcgggg atgtgcaccg cgatggtctc
    10681 gtcgaccggc tcgtacacct tgggccggcg gccgaccttg cggatgcccg tgggcgcttc
    10741 gtagtgggcc tggagcaggc cggccgccac cagcttgtcc aggtggaagg cggccggctt
    10801 gcgggagatg ccgaggccct cggccacctc ctcgggcgtc accggccgcc gctcccgccg
    10861 gatgagctcg aacacgcggc gtcgcgcggc gtcgcgtggc gtcgcgtggc gtcgccggtg
    10921 gtcacccgct cagtgagaac ccccgagtgg tggggtcgcg gcagcccacc cggtcgtacg
    10981 ccactgcacc gctctgcggc gcgccgccgc ggtcgagcgc acgtcccggc cgggtcctgc
    11041 ggcgcacctc ggccaggtcg gtgccgtgtc ccgcagagcg caccgcacac ggccgagggc
    11101 ctggcggatc gacgtccccg ggacgtcgat accgacacgc tcctcggcgt aggcacggac
    11161 cagcgtgtgc atgcggaagc gtccgggttc cgtctccgcc agaagatgcg tcgcggacag
    11221 cgcgtcgagg tgctcgcgca cggtctgttc ccgttcaccc gcgagagcga cggcgcaggc
    11281 gacgcccgta cggtcacggg ggtgaagcgc caggaggcgc agcaaccgcc gggcccccgg
    11341 ggcgacctgt gcgtcggagg cggccagtgc tcggcgtacg ccgccctcca gggcgagggt
    11401 gatcacggtg tcgtggtcgt agtcgtcgag tgtccatccg gggtgtcgcc gtatgtggtg
    11461 gccgacggcg gtcagcgcca ggggcaggtg tccgaggtcg tcggcgatcc gccgcagggc
    11521 ggtggggtcc gcggtgacgg cttcggcccc ggccgtccgc cgcaggagtt cgaccgagtc
    11581 ggccgctgcc ggcggtccga cggtgcgtcg ggtggcatcg gggaggccgt gcagcagacg
    11641 gcggctggtg atcagggtgg cgctggccgg gtcgccgggg aggaggggcc ggacctgctc
    11701 ggcgtccgcg gcgtcgtcga gcacgacgaa cgcgccggtg ccggcgagga gttgccggta
    11761 gcggcgggcc cgggcctccg tgccgtgcgg gatgcgttct ccggcggcgc ccagcaggcg
    11821 caggaaggta tccagcaccg ccgcggtgtc ggcgggggga cggtccggtg cgaagccgcg
    11881 caggtcggcg aagagggcgg gggcgtcggc gtgcttccgg gcctgtgcg
//
