LOCUS       NZ_JACHGT010000002     10423 bp    DNA     linear   CON 26-SEP-2024
DEFINITION  Phytomonospora endophytica strain YIM 65646 Ga0310468_02, whole
            genome shotgun sequence.
ACCESSION   NZ_JACHGT010000002
VERSION     NZ_JACHGT010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Phytomonospora endophytica
  ORGANISM  Phytomonospora endophytica
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Phytomonospora.
REFERENCE   1  (bases 1 to 661514)
  AUTHORS   Goeker,M.
  TITLE     Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing
            the most valuable type-strain genomes for metagenomic binning,
            comparative biology and taxonomic classification
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 661514)
  AUTHORS   Goeker,M., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 173.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014202425.1-RS_2024_09_26
            Annotation Date                   :: 09/26/2024 09:39:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,840
            CDSs (total)                      :: 7,783
            Genes (coding)                    :: 7,704
            CDSs (with protein)               :: 7,704
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 21 of 79
            Pseudo Genes (incomplete)         :: 67 of 79
            Pseudo Genes (internal stop)      :: 5 of 79
            Pseudo Genes (multiple problems)  :: 14 of 79
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:30:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 583985
            Orig. end                         :: 594408
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACHGT010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5423
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    157..166
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            1198..1875
                     /locus_tag="HNR73_RS05980"
                     /old_locus_tag="HNR73_001221"
     CDS             1198..1875
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HNR73_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HNR73_001221"
                     /product="hypothetical protein"
                     /protein_id="WP_184786237.1"
                     /transl_table=11
                     /translation="MKVRRIAAKAAIATVGLLAVAGLSACRVESGAAVFVQDQRIAETQ
                     VDAVVDSAPEAFGNDIGPIRQWTVSSFAVVELARQYAADNGTALPGPDYAGVAEAAGGE
                     SAFTRLYAEQAAYGQTLFTEATAVQPTEADVADLRIGTFSQINASLDAEQAAYVEEAIS
                     QAATGEAFSQSLGKRDLLNSLFAEYDVVANPRYGAPTVTVAQVAVAPQVAYSVTVPLPT
                     TAS"
     gene            1880..2872
                     /locus_tag="HNR73_RS05985"
                     /old_locus_tag="HNR73_001222"
     CDS             1880..2872
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904797.1"
                     /locus_tag="HNR73_RS05985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001222"
                     /product="MazG family protein"
                     /protein_id="WP_184786238.1"
                     /transl_table=11
                     /translation="MAPRIVLLVTSPRLPAGILSADAWDELRRAQAVFTGADSAQANAI
                     RRVGVNVTVIDEHTAADVLLSTVDGATTVWLSGPDGDPRLARTLGTRLAKNPSAAEIEL
                     CHGSWDPPGGRLLDVVSTMDTLRSPGGCPWDAEQTHESLLPYLVEETYEVIDAVHSGDK
                     EHLREELGDLLLQVAFHARMAQDDPDEETRFGIDDVSGDLVAKLVRRHPHVFADVEVTD
                     AGQLNDNWEAIKAEEKPHRESAMDGVAMGQPALPLSAKLQSRAERAGLAEAAWPEDGTG
                     AELTEETLGARLFALVARAREAGLDAELALRRAALDFAERVRAAEKDAA"
     gene            3080..3655
                     /gene="ectA"
                     /locus_tag="HNR73_RS05990"
                     /old_locus_tag="HNR73_001223"
     CDS             3080..3655
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664377.1"
                     /locus_tag="HNR73_RS05990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001223"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_184786239.1"
                     /transl_table=11
                     /translation="MSPDDASATVAQVPGNLAVTLSTPSIPDGNHLWRLAREAGSLDVN
                     SPYSYLLWCRDFADTSVVARDGERPVGFISGYTRPGRAATLFVWQVAVAPSHRRQGLAR
                     RMLDHLADSLAPRGVVNVEATVTPSNESSTRLFTSFAEARGATVTRDVLFAEDHFPAGG
                     AHEAEVLFHIGPLRRAATPPPGNGSAIG"
     misc_feature    complement(3738..3753)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.82"
                     /tool="antismash"
     gene            3745..5004
                     /gene="ectB"
                     /locus_tag="HNR73_RS05995"
                     /old_locus_tag="HNR73_001224"
     CDS             3745..5004
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-358). E-value: 4.4e-73.
                     Score: 238.2. Matches aSDomain:
                     nrpspksdomains_HNR73_RS05995_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084079.1"
                     /locus_tag="HNR73_RS05995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001224"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_184786240.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.2e-73, bitscore:
                     241.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIFEETESQVRSYCRGWPALFVRSKDSHVFDEHGKSYLDFFAGA
                     GALNYGHNHPVLKNALLEYLTDDHIVHSLDMYTEAKARFLETFRDVILRPRGLEYRVQF
                     PGPTGTNAVEAALKIARKVTGRTTIARFTNSFHGMTLGSLAVTGNGRKRHGAGVPLEHT
                     LTLPFDQYLDGSTPDLLWLRRLIEDSSSGVGLPAAVIVETVQGEGGINVASPEWLRALS
                     LLCSEHDMLLIVDDVQMGCGRTGPFFSFEESGVVPDVVCLSKSLSGYGLPFAVTLLRPE
                     LDIWEPGEHNGTFRGYNPAFVTATAALDEFWRDGILRDTVDRNGSFVDSELRLIADEHA
                     DAGATVRGRGLARAVAFEDANIAGAVCRNAFERGLLLETAGSDDEVVKLLPPLTTHRED
                     LDAGLSIVADSVRAARKELA"
     aSDomain        3811..4818
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HNR73_RS05995_Aminotran_3.1"
                     /evalue="4.40E-73"
                     /label="HNR73_RS05995_Aminotran_3.1"
                     /locus_tag="HNR73_RS05995"
                     /protein_end="358"
                     /protein_start="22"
                     /score="238.2"
                     /tool="antismash"
                     /translation="VRSKDSHVFDEHGKSYLDFFAGAGALNYGHNHPVLKNALLEYLTD
                     DHIVHSLDMYTEAKARFLETFRDVILRPRGLEYRVQFPGPTGTNAVEAALKIARKVTGR
                     TTIARFTNSFHGMTLGSLAVTGNGRKRHGAGVPLEHTLTLPFDQYLDGSTPDLLWLRRL
                     IEDSSSGVGLPAAVIVETVQGEGGINVASPEWLRALSLLCSEHDMLLIVDDVQMGCGRT
                     GPFFSFEESGVVPDVVCLSKSLSGYGLPFAVTLLRPELDIWEPGEHNGTFRGYNPAFVT
                     ATAALDEFWRDGILRDTVDRNGSFVDSELRLIADEHADAGATVRGRGLARAVAFE"
     gene            5001..5423
                     /locus_tag="HNR73_RS06000"
                     /old_locus_tag="HNR73_001225"
     CDS             5001..5423
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="HNR73_RS06000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001225"
                     /product="ectoine synthase"
                     /protein_id="WP_184786241.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-52, bitscore:
                     171.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLEEIAGTDRDVVGNDRDRAQGTWRSLRLVLAKEKVGFSLH
                     DTVLYAGTKTSMWYANHIEAVYVIDGEGELIDHDNGESHRLAPGTMYLLDGHEKHTLHA
                     HTDLRTVCVFNPPVTGREVHDADGVYPLLTETGEES"
     gene            5423..6325
                     /gene="thpD"
                     /locus_tag="HNR73_RS06005"
                     /old_locus_tag="HNR73_001226"
     CDS             5423..6325
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR02408.1"
                     /locus_tag="HNR73_RS06005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001226"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_184786242.1"
                     /sec_met_domain="PhyH (E-value: 1.8e-35, bitscore: 118.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDTVHGVRDLYPTRVGAEAALLYRKEDPVVYGRRNEGLLDQETL
                     DSYEENGFLSVEELITPAELLGYQAELERLLTDEAIKRDERTVLEKDSGEVRSIFEVHK
                     ISEVIAGLVADERVVGRARQILGSDVYVHQSRINYKPGFKGKEFYWHSDFETWHAEDGM
                     PRMRAVSISIALTDNHAYNGGLMIMPGSHKTFVSCVGETPADHYRESLREQEIGTPDPL
                     SLAVLAEKHGIELFTGPAGSATMFDCNCMHGSNGNITPFARSNVFVVFNSVDNTCVAPY
                     AAPGRRPDFVAARDFTPVK"
     gene            complement(6309..7331)
                     /locus_tag="HNR73_RS06010"
                     /old_locus_tag="HNR73_001227"
     CDS             complement(6309..7331)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HNR73_RS06010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="HNR73_001227"
                     /product="hypothetical protein"
                     /protein_id="WP_184786243.1"
                     /transl_table=11
                     /translation="MTAFPRRAAIVAAVATTVYLALRVYWAAGGVLGLTVCAPGESDRA
                     ALASGCDADTAVLGFWPGWGAVGLAALAVGVVAAAPRRGTVTEAGLWGATAAFVVVSFP
                     GHLLFQIPAGLAGRATDWRDVVHRLAQLGLALLLARVAVASAKSRRCGHDRRQGTSPVA
                     RWARRSAYAACALPVLGFSVPHLLWVLGIPFGASEQTLTEIRTDLDPATAIALCAVPAL
                     GGFVSLGLGMRWGRVLFGRRIPRMLALVPGAVVSAALTAYGVIGIALMTRDLSAGTATW
                     TDLAREWSIFGTELVFLAWGLALSAATWGYHLAARDRCGTCERADDGVPPPAPSVTSPA
                     "
     gene            complement(7448..8365)
                     /locus_tag="HNR73_RS06015"
                     /old_locus_tag="HNR73_001228"
     CDS             complement(7448..8365)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440845.1"
                     /locus_tag="HNR73_RS06015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001228"
                     /product="glycosyltransferase"
                     /protein_id="WP_184786244.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.2e-19,
                     bitscore: 63.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEQEKSWPSVGVVVPTHRRPQQLRDTLDAILTQDYPGEMHIVVV
                     FDRAKPDESFVQDGPRSVRVTKNLRTPGLAGARNTGIDALGTDLIAFCDDDDVWLPGRL
                     RRQVAALSPNAELVTCAVEVAYEGAVVPRLAGMATIRHVDLLRSRMMMLHSSTFLLRRA
                     ALLGGLGMVAEDAPGSQNEDWDLLLRAARRAPITHVDEPLVRVHWGNSHFAARYDTKIS
                     SLHWMLSRHPELHGCPQGAARVYGQLACWHAAVGQRKDALQWARRAMRSKWTEPRAVIA
                     TAAAVGLVRIETVMSALHRKGHGI"
     gene            complement(8366..9451)
                     /locus_tag="HNR73_RS06020"
                     /old_locus_tag="HNR73_001229"
     CDS             complement(8366..9451)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (extras)
                     Sulfotransfer_3: Sulfotransferase domain"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687874.1"
                     /locus_tag="HNR73_RS06020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001229"
                     /product="sulfotransferase"
                     /protein_id="WP_184786245.1"
                     /transl_table=11
                     /translation="MSEVVRVLFIGGLGRSGTTLLERLLGQLPGTLPLGEVAHLWERDI
                     RDDERCACGARFSMCEFWQRVGRRAFNGWENVDLARIEALRATVDRTRHIPTLAAKKLS
                     PVQHSLITEYAEFYRRVYAAASADAGGGRLIIDSSKHASLAYCLRWCPDVDLRVVHVVR
                     DSRGVAYSWTKQVSRPETGGQAEMTRYSPARSALLWNAQNAAFSMLGRRGVPVRRVRYE
                     KLLADPRTVVERLAEFAGVELAPTDLRYIGEDYADLGPNHSAAGNPMRFSVGRIPLRRD
                     EEWREELPSTQQRLVKTLTAPLLNRYGYTTRPVEPPEAAPAPDVPGPTVALPLPMQSAP
                     ADSRPEPIGPVAIAPVSVGRE"
     misc_feature    complement(9548..9561)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.97"
                     /tool="antismash"
     gene            9599..10390
                     /locus_tag="HNR73_RS06025"
                     /old_locus_tag="HNR73_001230"
     CDS             9599..10390
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520343.1"
                     /locus_tag="HNR73_RS06025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNR73_001230"
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="WP_184786246.1"
                     /transl_table=11
                     /translation="MSSQPTFQSRLETSGSHFTVAGIRFDALDERGVVAHVRRALKEGH
                     GGRIVTANVDIVRQALKNPEARAHVEAGTLVVADGAPVVWASKLARRGLPARVAGSDLI
                     WSLSGELGQLGGSVFLLGGRPGSELLAARRLMDASPGLRIAGALAPPMGFEKDPELVRR
                     VCEEVAAAKPDMVYVGLGFPKQEWMISKLAPLLPGAWFMGCGASIAFVAGTLRRAPLWM
                     QRAGLEWLHRMAREPRRLFARYVLHDAPLALRLLLLALLRR"
ORIGIN
        1 cgagatgtcg acgatcgcga ccccgcccga ggagcgccac cccgtcctga ccagcgtcgg
       61 cgcctacgac gaacgccaga tcaccgccgc gatccggcgc gagctgctcc gcgacgggca
      121 ggtgttcttc ctgcacaacc gggtcgagtc gatcgagaag gcggcccgcc gcatccgcga
      181 gctcgtaccc gaggcgcgcg tggtcaccgc ccacggaaag atgggcgagg accgcctgga
      241 gaagatcatg gtcggcttct gggagaacga gtacgacgtg ctcgtggcga ccacgatcat
      301 cgagtcgggc atcgacatcc ccaacgccaa caccctgatc gtggagcgcg ccgacctgct
      361 cggcctgtcg cagctccacc agatccgcgg gcgcgtcggg cgcggccgcg aacgcgcgta
      421 ctcgtacttc ctgtacccgc cggacaagcc gctgaccgag caggcccacg agcgcctcgc
      481 cacgatcgcg cagcacaccg agctgggcgc cggcatgtac gtggcgatga aggacctgga
      541 gatccgcggc gccgggaacc tcctcggcgg ggaacagtcc gggcacatcg ccggggtcgg
      601 gttcgacctg tacgtgcgga tggtcggcga cgcggtggcg tccttcaagg gcgacaagcg
      661 cgaaccgctg caggaggtca aggtcgacct gccggtcgac gcgaacatcc cctacgacta
      721 catctcggtg gagcggctgc ggctggagat gtaccgcaaa ctcgccgagg tccgcaccga
      781 ggagcacctg gccgaggtgc gcgccgagat gaccgaccgc tacggggagg cgcccgaacc
      841 ggtcgacaac ctggtggagg tcgcgcgttt ccggctgctg ctgcgccggt acgggctcac
      901 cgacgtggcc ctccagggcc ggcacatccg cttctcgccg atggacctgc ccgactccag
      961 gcagatgcgg ctcaagcgct actaccccga cgcggtgtac aagaccaccg gcgacgtcgt
     1021 gtccctgccc cggccgacga ccgggcgcat gggcggggcg cccctgtcgg gattgccgct
     1081 gctcaagtgg gccggggagc tcgtgacggg cgtgctcgac gcgccgcagc cggtctcgtg
     1141 aagctcggat aggctgcctt cggtttggaa ttgactgagc agacagcgag ggacgacgtg
     1201 aaggtacgac gcatcgccgc gaaggcagcc atcgctaccg tggggctgct ggccgtggcc
     1261 gggctgtcgg cctgtcgggt cgagtcgggg gccgccgtgt tcgtccagga ccagcgcatc
     1321 gccgagacgc aggtcgacgc ggtcgtggac agcgcccccg aggccttcgg caacgacatc
     1381 gggccgatcc gccagtggac ggtcagctcc ttcgccgtcg tcgaactggc ccgccagtac
     1441 gccgccgaca acggcaccgc actgcccggc cccgactacg ccggcgtcgc cgaggccgcg
     1501 ggcggcgaaa gcgccttcac acgcctgtat gccgaacagg ccgcctacgg ccaaaccctg
     1561 ttcaccgagg ccaccgccgt gcagcccacc gaggccgacg tggccgacct gcgcatcggc
     1621 acgttcagcc agatcaacgc gtccctcgac gccgaacagg ccgcctacgt cgaagaggcc
     1681 atctcccagg cggccaccgg cgaggccttc agccagtccc tcggcaagcg cgacctgctc
     1741 aacagcctgt tcgccgagta cgacgtggtc gccaacccgc gctacggcgc cccgaccgtc
     1801 acggtcgcgc aggtcgcggt ggcgccgcag gtcgcctact cggtgacggt tccgcttccc
     1861 actaccgcgt cctgacgcca tggcaccgcg catcgtcctc ctcgtcacct caccgcgact
     1921 gcccgccggg atcctgtcgg ccgacgcgtg ggacgagctg cgccgcgccc aggccgtctt
     1981 caccggcgcc gactccgcgc aggccaacgc gatccgccgg gtcggcgtca acgtcaccgt
     2041 gatcgacgag cacaccgccg ccgacgtcct gctgtccacc gtggacggtg ccacgaccgt
     2101 gtggctgtcc ggccccgacg gcgacccgcg cctcgcgcgc accctcggca cacggctggc
     2161 gaagaacccc tcggcggccg agatcgaact gtgccacggg tcctgggacc cgccgggcgg
     2221 ccgcctcctc gacgtcgtgt ccaccatgga caccctgcgc tcgccgggcg gctgcccgtg
     2281 ggacgccgag cagacccacg agtcgctgct gccctacctg gtcgaggaga cctacgaggt
     2341 catcgacgcc gtgcactccg gcgacaagga gcacctgcgc gaggaactgg gcgacctgct
     2401 gctccaggtc gcgttccacg cacggatggc gcaggacgat cccgacgagg agacgcgctt
     2461 cgggatcgac gacgtgtcgg gcgacctcgt cgccaaactc gtgcggcggc atccccacgt
     2521 gttcgccgac gtcgaggtga ccgacgccgg acagctcaac gacaactggg aagccatcaa
     2581 ggccgaggag aagccgcatc gcgagtcggc gatggacggt gtcgcgatgg gccagcccgc
     2641 gctgcccctg tcggcgaagc tccagtcgcg ggccgagcgc gcgggactgg ccgaggccgc
     2701 ctggcccgaa gacggcaccg gtgccgaact gaccgaggag acgctcgggg cgcggttgtt
     2761 cgcgctcgtg gcgcgggcgc gcgaggccgg gctcgacgcc gaactcgcgc tgcgccgggc
     2821 ggccctcgac ttcgccgagc gggtccgcgc cgccgagaag gacgcggcgt agtccgcaag
     2881 tccccgaaaa gcccccaaag gcaccccgcg cattcacttt gtcgacaatc gacaggcaaa
     2941 ccggcagact gtccgatcct cgacagtctt cgtccgaaaa gcccgctacc tgcccttaat
     3001 atcccctttt agacctcgca aaagcgctca aaacaccagg ctggcttgac cttgctctga
     3061 tccgatagtg tcgttgacca tgtcccccga cgacgcaagc gcaaccgtcg cccaggtccc
     3121 cgggaacctc gccgtcaccc tttcgacccc ctcgataccc gacggcaacc atctctggcg
     3181 cctggcccgc gaggccggat ccctcgacgt caactccccc tacagctacc tgctctggtg
     3241 ccgcgacttc gccgacacca gcgtcgtggc gcgcgacggg gaacgaccgg tcggcttcat
     3301 ctccggctac acccggcccg gccgggccgc cacgctcttc gtctggcagg tggcggtcgc
     3361 cccgagccac cggcgacagg gcctggccag gcgcatgctc gaccacctcg ccgattccct
     3421 cgccccccgt ggcgtcgtga acgtcgaggc gacggtgaca ccgtccaacg aatcgtccac
     3481 gcggctgttc acctccttcg cggaagcgag gggggcgacg gtcacgcgtg acgtgctgtt
     3541 cgccgaggac catttcccgg ccggcggcgc ccacgaggcg gaggtcctgt tccacatagg
     3601 acccctgcgc cgagcggcga cgcccccacc ggggaacggt tccgccatcg ggtgatgccg
     3661 acccgatgac gtgccgcacc gccccccggc aggggcggtg cgcgacgacg gcgaccggcc
     3721 agtacaggcc gaccctggag gataatgcgc atattcgaag aaaccgagtc gcaagtccgc
     3781 tcctattgcc gcggctggcc cgcgttgttc gtccgcagca aggacagtca cgtcttcgac
     3841 gagcacggga agtcctatct ggacttcttc gccggcgcgg gtgccctcaa ctacgggcac
     3901 aaccatccgg tgctcaagaa cgcgctgctg gagtacctga ccgacgacca catcgtccac
     3961 tccctcgaca tgtacaccga ggccaaggcc cgcttcctgg agaccttccg ggacgtgatc
     4021 ctgcgcccgc gcggccttga gtaccgcgtg cagttcccgg gcccgaccgg gacgaacgcc
     4081 gtcgaggcgg ccctcaagat cgcccgcaag gtcaccgggc gcacgacgat cgcccggttc
     4141 accaactcct tccacggcat gaccctcggg tcgctcgcgg tcaccggcaa cgggcgcaag
     4201 cggcacggcg cgggcgttcc cctcgaacac acgctcacgc ttcccttcga ccagtacctc
     4261 gacgggtcca ccccggacct gttgtggctg cggcgcctga tcgaggactc cagcagcggc
     4321 gtcggcctcc cggccgccgt catcgtggag accgtccaag gggagggcgg catcaacgtg
     4381 gcgagcccgg agtggctgcg cgccctgtcg ctgctgtgct ccgagcacga catgctgctc
     4441 atcgtcgacg acgttcagat gggctgcggc cgcaccggcc cgttcttcag cttcgaggag
     4501 tccggcgtcg tgcccgacgt cgtgtgcctc tcgaaatccc tgagcggcta cggcctgccc
     4561 ttcgcggtca cgctgctgcg cccggagctc gacatctggg agccgggcga gcacaacggc
     4621 accttccgcg gttacaaccc ggcgttcgtg acggcgaccg ccgcgctgga cgagttctgg
     4681 cgcgacggga tcctgcgcga caccgtcgac cggaacgggt cctttgtgga ctccgaactg
     4741 cggctgatcg ccgatgagca cgccgacgcc ggagcgaccg tgcgcggtcg cggcctggcg
     4801 cgggcggtgg ccttcgagga cgcgaacatc gcgggggcgg tgtgccgcaa cgccttcgag
     4861 cgcggcctgc tgctggagac ggccggaagc gacgacgagg tggtcaagct gctcccgccg
     4921 ctgaccacgc accgggagga tctggacgcc ggactgtcca tcgtggccga ttcggtccgg
     4981 gcggcgagga aggaactggc atgatcgtgc gcactctgga agagatcgcg ggcaccgacc
     5041 gcgacgtcgt cggcaacgac cgggaccggg cgcaggggac gtggcgttcg ctgcggctcg
     5101 tgctggccaa ggagaaggtc ggcttctccc tgcacgacac cgttctctac gcgggtacga
     5161 agacctccat gtggtacgcc aaccacatcg aggccgtcta cgtcatcgac ggcgagggcg
     5221 agctcatcga ccacgacaac ggtgagagcc atcgcctggc gcccgggacg atgtacctgc
     5281 tcgacgggca cgagaagcac acgctgcacg cgcacaccga tctgcggaca gtctgcgtgt
     5341 tcaacccgcc ggtgaccggc cgcgaagtgc acgacgccga cggcgtctac cccctgttga
     5401 ccgagaccgg agaggaaagc tgatgttcga caccgttcat ggagtccgcg acctgtaccc
     5461 gacccgggtc ggcgccgagg cggcgctgct ctaccgcaag gaggacccgg tcgtctacgg
     5521 ccggcgcaac gaggggctgc tcgatcagga gaccctcgac tcctatgagg agaacggttt
     5581 cctgtcggtc gaggagctga tcacgccggc cgagctgctg gggtaccagg cggagctgga
     5641 gcgactgctc accgacgagg ccatcaagcg cgacgagcgg acggtgctgg agaaggactc
     5701 cggcgaggtc cggtcgatct tcgaggtgca caagatcagc gaggtgatcg ccggtctcgt
     5761 cgccgacgag cgcgtcgtcg ggcgggcccg ccagattctg ggttcggacg tctacgtgca
     5821 ccagtcgcgg atcaactaca agcccggttt caagggcaag gagttctact ggcattccga
     5881 cttcgagacc tggcacgccg aggacgggat gccgcggatg cgcgcggtga gcatctcgat
     5941 cgcgctgacc gacaaccacg cctacaacgg cgggctgatg atcatgccgg gttcgcacaa
     6001 gaccttcgtc tcgtgcgtcg gcgagacccc ggccgatcat taccgggagt ccctgcggga
     6061 gcaggagatc gggacgcccg acccgctgag tctggcggtg ctggccgaga agcacgggat
     6121 cgagctgttc accggcccgg cggggtcggc gacgatgttc gactgcaact gcatgcacgg
     6181 ctcgaacggc aacatcacgc ccttcgcgcg gtcgaacgtg ttcgtcgtgt tcaacagcgt
     6241 cgacaacacg tgcgtggccc cgtacgccgc tccggggcgc cgccccgact tcgtcgcggc
     6301 ccgggacttc acgccggtga agtgaccgag ggggcgggcg gcgggacgcc gtcgtccgcc
     6361 ctttcgcatg tgccgcagcg gtcgcgggcg gcgaggtggt atccccacgt cgccgccgac
     6421 agcgcgaggc cccaggcgag gaagacgagt tcggtgccga agatcgacca ttcgcgggcc
     6481 aggtcggtcc acgtcgcggt accggccgac aggtcgcggg tcatcagcgc gattccgatg
     6541 acgccgtagg cggtcagggc cgccgagacg accgcgcccg gtacgagggc gagcatccgg
     6601 gggatcctcc gtccgaagag gacacgtccc cagcgcatgc cgaggccgag gctgacgaac
     6661 ccgccgagcg ccgggacggc gcacagggcg atggcggtgg cggggtcgag gtcggtgcgg
     6721 atctcggtga gggtctgctc cgaggcgccg aaggggatcc cgaggaccca cagcaggtgc
     6781 gggacgctga agccgaggac gggtagggcg caggcggcgt acgccgaccg gcgcgcccag
     6841 cgcgcgaccg gcgaggtccc ttgtcgccgg tcgtgcccgc agcggcgcga cttcgccgac
     6901 gcgacggcca cccgtgcgag cagcagcgcg agtccgagct gcgcgagccg gtgtacgacg
     6961 tcgcgccagt ccgtcgcccg ccccgccagc ccggcgggaa tctggaagag caggtgcccg
     7021 ggaaacgaga cgacgacgaa cgcggccgtc gcgccccaca acccggcctc ggtcaccgtg
     7081 ccccgccgcg gcgcggcggc cacgacaccg acggcgagcg cggcgagtcc gacggcaccc
     7141 caccccggcc agaacccgag gacggcggtg tcggcgtcgc acccggacgc gagggcggcg
     7201 cggtcggact caccgggggc gcagacggtg aggccgagga cgcccccggc ggcccagtag
     7261 acgcggagtg cgaggtagac ggtggtggcg acggccgcta cgatcgcggc ccggcgcggg
     7321 aatgcggtca tgggggccag actcgcgcgc gggcggcggc gcggcatgac cgtggtgggg
     7381 gacgggggtc ccccgggtgg gtgaaatgtc agacgcggta ttcggccgcg cgcgccggtg
     7441 gccgccccta aatcccatgc cccttccggt gcagcgccga catcaccgtc tcgatgcgca
     7501 ccagacccac cgccgccgcc gtcgcgatga ccgctcgggg ctccgtccac ttcgagcgca
     7561 tcgcgcggcg ggcccattgc agggcgtcct tgcgttgccc gacagcggcg tgccagcagg
     7621 cgagctgccc gtagacgcgt gccgcgccct gcgggcagcc gtgcagttcc gggtgcctgc
     7681 tcagcatcca gtgcagcgag ctgatcttcg tgtcgtagcg ggccgcgaag tgcgagttgc
     7741 cccagtggac gcgcacgagc ggttcgtcga cgtgcgtgat cggcgcgcgg cgggccgcgc
     7801 gcaggagcag gtcccagtcc tcgttctggc ttcccggggc gtcttcggcg accatcccga
     7861 gccctccgag cagggcggcc cggcgcagca ggaacgtcga cgagtgcagc atcatcatcc
     7921 gcgagcgcaa caggtccacg tgccggatcg tcgccatccc cgcgagccgg ggcacgacgg
     7981 cgccctcgta ggcgacctcg acggcgcagg tgaccagctc ggcgttcggc gacagcgcgg
     8041 cgacctggcg gcgcagccgt ccgggcagcc agacgtcgtc gtcgtcgcag aaggcgatca
     8101 gatccgtgcc gagggcgtcg atgccggtgt tgcgggcacc ggcgaggccg ggcgtccgca
     8161 ggttcttcgt gacccgcacc gaacggggcc cgtcctgcac gaaactctcg tcgggtttgg
     8221 cgcggtcgaa caccacgacg atgtgcatct cacccgggta gtcctgggtg aggatcgcgt
     8281 cgagggtgtc gcgcaactgc tgcggccggc ggtgggtcgg cacgacgacg ccgaccgagg
     8341 gccagctctt ctcctgctcc gacacctact cccgccccac actgaccggg gcgatggcga
     8401 cgggcccgat cggctcgggc cgcgagtcgg cgggcgccga ctgcatcggc agcggcagcg
     8461 ccaccgtcgg cccgggcacg tccggcgcgg gggccgcctc cggtggctcg acgggccgcg
     8521 tcgtgtaccc gtagcggttg agcaggggcg cggtcagggt cttcacgaga cgctgctgcg
     8581 tcgacggcag ctcctcgcgc cactcctcgt cccggcgcag cgggatccgc ccgaccgaga
     8641 agcgcatcgg gttgcccgcc gcgctgtggt tcgggccgag gtcggcgtag tcctcaccga
     8701 tgtagcgcag gtcggtcggc gcgagctcga ccccggcgaa ctccgcgagg cgctcgacga
     8761 cggtccgcgg gtcggccagc agcttctcgt agcgcacccg ccgcaccggg accccgcgcc
     8821 gccccagcat cgagaaggcc gcgttctgcg cgttccacag cagcgccgac cgcgccgggg
     8881 agtaccgcgt catctccgcc tgcccgcccg tctcgggccg cgacacctgc ttcgtccacg
     8941 aataggcgac accgcggctg tcgcgcacga cgtgcaccac gcgcagatcc acgtcgggac
     9001 accagcgcag gcagtaggcc agcgacgcgt gcttcgagga gtcgatgatc aaccggccgc
     9061 caccggcgtc ggccgaggcg gcggcgtaca cgcgccggta gaactcggcg tactcggtga
     9121 tcagcgaatg ctgcaccggc gagagcttct tcgccgcgag ggtcgggatg tgccgggtgc
     9181 ggtccacagt ggcccgaagc gcctcgatcc gcgccaggtc gacgttctcc cagccgttga
     9241 aggcacgccg cccgacccgc tgccagaact cgcacatgga gaaacgcgcg ccacaggcgc
     9301 agcgctcgtc gtcgcggatg tcacgctccc acaggtgcgc gacctcgccc agcggcagcg
     9361 tccccggcag ctgaccgagc agcctctcca gcagggtggt cccgctccgg ccgagcccgc
     9421 cgatgaacaa cacccgtacg acctctgaca tacctgtccc ccacggtgcg tccggcgggg
     9481 tcaccgccgg agggcggtgg cagccctcac ctcctataac cgatcgaccc caccgaaagg
     9541 acacgccttg tgtcaaaaca actacgatta gctccttcgc gggttaaggt ctgcggctat
     9601 gagctcgcag cccacgttcc agagtcgcct ggaaactagc ggtagtcatt tcaccgtcgc
     9661 cggtatcaga ttcgacgcgc tcgatgagcg gggagtggtc gcgcacgtgc gccgggcgct
     9721 caaggaggga cacggcggcc ggatcgtcac ggccaatgtg gacatcgtcc gccaggcctt
     9781 gaagaacccc gaggcgcgcg cccacgtcga ggccggaacc ctcgtcgtcg ccgacggcgc
     9841 acccgtcgtc tgggcgtcca aactggcccg tcgcggcctc cccgcgcggg tcgcgggctc
     9901 cgacctgatc tggagccttt cgggcgaact ggggcaactg ggcgggtcgg tgttcctgct
     9961 cggtggacgc cccggcagcg agttgctggc cgcgcggcgg ctgatggacg ccagtcccgg
    10021 cctgcggatc gcgggcgcgc tggcaccgcc gatgggcttc gagaaggatc ccgagctcgt
    10081 ccggcgggtc tgcgaggagg tcgcggcggc caaaccggac atggtctacg tcgggctcgg
    10141 cttccccaag caggagtgga tgatctccaa actggcgccc ctgctgcccg gcgcgtggtt
    10201 catgggctgc ggcgcctcga tcgccttcgt cgcgggcacc ctgcggcgcg ctccactgtg
    10261 gatgcagcgg gccgggctcg aatggctgca ccggatggcc cgcgaaccgc gccggctctt
    10321 cgcccgctac gtcctgcacg acgcgccgct cgcactgcgc ctgctgctgc tggccctgct
    10381 gcgccgctag gcgcacacgt gcggccgaac cggcccggac gag
//
