LOCUS       NZ_JABBXA010000001     10270 bp    DNA     linear   CON 07-NOV-2024
DEFINITION  Microbispora sp. H13382 NODE_1, whole genome shotgun sequence.
ACCESSION   NZ_JABBXA010000001
VERSION     NZ_JABBXA010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora sp. H13382
  ORGANISM  Microbispora sp. H13382
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Streptosporangiales; Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 845565)
  AUTHORS   Liu,C.
  TITLE     Selective isolation, identification and anti-microbial activity
            study of Microbispora
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 845565)
  AUTHORS   Liu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2020) Microbiological Resources Collection Center,
            Sichuan Industrial Institute of Antibiotics, 168 Huaguan Road,
            Chengdu, Sichuan 610052, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.5
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014161445.1-RS_2024_11_06
            Annotation Date                   :: 11/06/2024 07:22:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,982
            CDSs (total)                      :: 7,906
            Genes (coding)                    :: 7,786
            CDSs (with protein)               :: 7,786
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 21 of 121
            Pseudo Genes (incomplete)         :: 104 of 121
            Pseudo Genes (internal stop)      :: 8 of 121
            Pseudo Genes (multiple problems)  :: 11 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:45:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 244662
            Orig. end                         :: 254932
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JABBXA010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10270
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10270
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10270
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5270](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5270
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    280..294
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.71"
                     /tool="antismash"
     misc_feature    complement(1556..1568)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 19.2"
                     /tool="antismash"
     misc_feature    2394..2408
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.93"
                     /tool="antismash"
     misc_feature    complement(2897..2912)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.67"
                     /tool="antismash"
     misc_feature    3060..3069
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    complement(3172..3192)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.7"
                     /tool="antismash"
     misc_feature    3407..3421
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.1"
                     /tool="antismash"
     misc_feature    3796..3812
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 21.87"
                     /tool="antismash"
     gene            complement(4215..4403)
                     /locus_tag="HHX41_RS00970"
     CDS             complement(4215..4403)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHX41_RS00970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_182904437.1"
                     /transl_table=11
                     /translation="MRALAWTCGCRAVVYELLQSGGQAFIRKTTQAEPTPKVQETHRWP
                     VEEARRVWHALLLGEMR"
     gene            complement(4528..4869)
                     /locus_tag="HHX41_RS00975"
     CDS             complement(4528..4869)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHX41_RS00975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_182904438.1"
                     /transl_table=11
                     /translation="MRQITNGNRTANQQAGILAALGDLLRSQGVRTRFISHITLRMTGF
                     PVQEKAGAELDVYGFGGQVATVTIDQENKPRRFLIKLPKSGTVVPLPSVDQPQSAVRWI
                     VGWANGGAQ"
     misc_feature    complement(4798..4800)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..5270
                     /locus_tag="HHX41_RS00980"
     CDS             5001..5270
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012610.5"
                     /locus_tag="HHX41_RS00980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_076437814.1"
                     /sec_met_domain="TIGR03651 (E-value: 8.2e-05, bitscore:
                     18.4, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEFRPDQLKWEAVLEEIRRRIRTGEYQVGHPIPGEPRMAEEFGI
                     AKGTARRVINELLKAGDVYTVLGKGTFVADPETGGPPRRDTEDE"
     gene            complement(5470..6867)
                     /locus_tag="HHX41_RS00985"
     CDS             complement(5470..6867)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547010.1"
                     /locus_tag="HHX41_RS00985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="replication initiator"
                     /protein_id="WP_182904439.1"
                     /transl_table=11
                     /translation="MSELPPLSPDVLAAAQRAAMPDFARWQEMVRATGGCAQPIRLQGR
                     RITFDAGLEVLDVYRTADEPTGYLLTACGNRRASRCPACSEVYRDDTYHLILSGLRGGK
                     GVPEDVSAHPRVFATFTAPSFGAVHARRMKGDKVLPCRPRRDNPVCEHGRPEGCGVRHA
                     DDDPRVGQPICVDCYDYRGAVLWNAHAGELWRRFTQALPAAFARHLGVSRAALRRRLRL
                     SYAKVAEYQARGLVHFHAVIRLDGPDGPSDRPPDWATVPVLQAAIRETAAAVSVPVPDD
                     APSFVGRWGTQLDVDPIYVAASLEGIADQRVAAYIAKYATKGAESAGTVDRPIREMADV
                     ARLEVTEHARRMIYTCFALANDPAYRLIPLRQWAHMLGYRGHFSTKSRHYSTTLGALRQ
                     IRADHQAERARERRGLPPRDERETVTVGQWRFAGSGYRHGEHLWAELIRQRIATARRIA
                     RERGEPA"
     gene            complement(6864..7019)
                     /locus_tag="HHX41_RS00990"
     CDS             complement(6864..7019)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHX41_RS00990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_182904440.1"
                     /transl_table=11
                     /translation="MAALAEEWCKRQLERSPRWGVNKWRRLAAIQGAEYVGDAHDTASD
                     SGRDAA"
     gene            complement(7525..9036)
                     /locus_tag="HHX41_RS00995"
     CDS             complement(7525..9036)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012464.5"
                     /locus_tag="HHX41_RS00995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="recombinase family protein"
                     /protein_id="WP_182904441.1"
                     /transl_table=11
                     /translation="MPEVVPVVSYARISSDGRGDEHGVQDQHKVNRQTAGRLGWTIVHE
                     FTDNDKSAAKANVVRDAFEAMVKALRAGRLADGTLIEGVVIVAEDRLARRPGDYERFVE
                     AFTYRDGRVFADARGPKDLYSEDTESMGLFGAVISKMEVRKMQRRMRRSHRARAEQGVP
                     VGGPRPFGWLADRVTIHPEEGPLLREAVDAIIAGGSLRAIVMKWQQLGVRTTRGNEWSR
                     RSLRATLSSPRLCGWREINGELVRDADGDPVMGKWEALITPDELLAVRAAFERTSREKA
                     DQYAALGMRFDQREHRYLLSGILRCGKPKADGTGICNAPLRVRPARGGVHLYVCPAPSD
                     GGCGGVGRRGDRVDVFISELVLAKLDESPDADTEDVPWAGEQELKDKKEQLAELTRQWN
                     ARTISNELFFMLVPSLEQRINELRREGQAYTAAKERKRARRIVDGADIRRRWYLPEDQG
                     GLSMGTKRAMVMEAFHAVIVHPVGKGMGSWGNFDPNALEPIWRED"
     gene            complement(9073..9210)
                     /locus_tag="HHX41_RS01000"
     CDS             complement(9073..9210)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HHX41_RS01000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_168066240.1"
                     /transl_table=11
                     /translation="MRRTLAAAVAATLAAGLAPSAAQATTGGGDRYKRVAYFIQWGIYG
                     "
     misc_feature    9245..9259
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.03"
                     /tool="antismash"
ORIGIN
        1 cctatttacg ctgactatat agacgatatc gctacagcaa cgccgtcttt ccgtgaccgt
       61 gaagcagaga tactcagtct ccagcggttc attaggtcgg atcagtttta ccaatggtgg
      121 attgcaggcc cgtggactgg aaaaactact ttggctgccc acctagccac acatccgcca
      181 aacgatactg atatcgtctc ctttttcgtg tctcgggtgc ggagccagca aacccggcaa
      241 tacttcgaga gtgtatgcga tcagttatcg gccctcctgg gaagagacgc tccgccctct
      301 tctggtcccg cagaattcat aagcctttgg aatgaggccg gtaggctggc cgagaagttg
      361 cagcggcatc tagtgttgct ggtagacgga ttagacgaaa acgacgaccc tacgccaatt
      421 gccgctcacc ttcctagcgt gcctcttccg cgctgtcatg ttgttgtgtt cagcagagag
      481 gggcctcctc tctctgaaag agttcccctt tctcatccac tacgcgccct aagtgcatct
      541 ggtgacatag ctattctaag cccgagtcca tatgcagagc gccttcgtga tctggcacac
      601 caagagattc gtgacctgct cctgaccagc gacgaaactt tcgtgcaaca gctactaggg
      661 ctacttgcgg cagctagccc tctcacacta ggtgagatcc tgacgctact tgagctggat
      721 gcaggagatg agcgaaggat agggcctcct atgtccccta tctcaatcga gcgaataatt
      781 acaagcaaag cagggcgagt actccgccct gtaatgactg ccgcgggaat ggcctatgca
      841 ttcgcacacg ccgagttgca acaagtcaca atcgataatc tcggacagcg aacgatcaat
      901 cactacgtga acctcgtgca tgcttgggcg gacgagtacg aacgcagggg atggccgata
      961 gatactcctg tctatctgat caatttctac ccgactctcc tcactgctca ccgggacatc
     1021 gaaagactgt cacgacttgc aacggagtct aggttggagc tcctgaagca aagaacggga
     1081 catgacggat tcggtgttca ggagctgaac tatgctttcg agctgatggt agaacgatcc
     1141 gacacggaca tcgagaaagc atgtacgtta gctatccagc ggcaacggct aattgaccgt
     1201 acggctcagt atcctccttc cgtcgttata gcgtccgctg ttttgcagca atggccacgg
     1261 gctctccact tcacgcgtac tatgcatgga gccttggaga agcttcatac tctgcgaggc
     1321 ctgtttgaag tcgctagccg gcaggggaag gatagggatt tcattcagag tttatataat
     1381 gacgtctaca aaacattcag cctacctgat gacatggagg aagtagacga ttattggctt
     1441 ggactagccg agataagcgc agaggcggga tatgtggatc gggccctaag cagcgctcgg
     1501 aaaatcggga atagacgtga gcaacttatc ctcctgtatg ccctagtggg ggaatttgca
     1561 aaatcaaatg agcaaactgc tgccgaggaa gcttttgcac aagcgatgcg tttggatcag
     1621 ggcagcgacc aaggtgtagc acgagctgat gctctagtcg cactcgccag catgtacagc
     1681 agggcgggcg accgactgcg atgcctcgac gtactaaagc aatcgattat tctggcgctt
     1741 gggtttgaag accaccttag ggactgggca ttgaatggca tcgctaggag ggctttggat
     1801 tgccaggccc ctgaggtggc ggtagatgca gcgacaaaaa ttaataatgg taatgcccgg
     1861 atggaagtgc ttcatctact aaacagaacg aagcacttcg accacgcatt gcgattggcg
     1921 aggaccttta aggatgcagc atcgcatgtt aaagccctgt gcgacgtcgc tttatctttg
     1981 ctgcaagccg gccaagttga gcgaggagtt gagcattttc gtaccgcaga agccaggtgc
     2041 cggaaagtaa aaaatgctaa agaccgagcg gtcctccttg catcggtagg cgctgttgcg
     2101 gcagaggggg gcgaggagga gttatctgaa cggctgcact atgagtcttt gtcgatagct
     2161 gacaccgaga cgaataggtc cagaagaaag gaaagctaca cactgatagc gcgtgaattc
     2221 gcccttagtg ggctgtatga cctggccgag aaaacagcaa acaaactcgg ggagtcgcaa
     2281 gatctagtta ctctactatg cttcgttgcc aagtgtgcgt acgaagatgg gagaaacctc
     2341 attggacagc ggcttatcca gagaagctta ggtgtggcaa gagagctgca gggtgaatat
     2401 ctttaccaat tcagggattc tccagacatt gcagcagaca tcgcaaaatt gaacatagta
     2461 aaaacttgcg tgcttgcagg aagcatgcaa gaagcagagc caatcttgga agagatttct
     2521 catgtgggga taaaaggaaa cgcctatcag cttctggctt ttgttgctga gcgactcgga
     2581 gaaagcgcgc gtgccgagag gttcgctact gaggccgatg acttgtggct ggatcttgat
     2641 tcggcatacg gcctgtctct ctcggatctg acagatgaca cttggtggac ttatcctacg
     2701 ctggatggtc ttctagactt taatcatgaa atcagcgtgc ttgctagcag tatcgcagaa
     2761 ggtggtgatc catgggaagc ggaccccgaa tatttatacc atgtaaaccg aatgtttgca
     2821 cgtatcgcca acgagtatgg gaaagcgcga gcatttgagt tggcccgcga agcgctaggt
     2881 cagatagatg attggttctg gtctagtgca gttgatgagc tttttatcat catggcgaag
     2941 gcgggcgagt ttatcaaggc tctgcgtttg cttcgggatg ttcggacagc ttttgatttg
     3001 gaagataagg cagaagggtt ggcggttctg gcttgggcga gcgcagaaaa taagctgccg
     3061 aaggcggcgc atgccatact tgagcgcgca gagagtagcg tacttgaaag ctttggcagg
     3121 actagcaggg gccccatttt ttcggatgaa ggttcaggct ggattgattc gaacgaagtc
     3181 ttggctatct ttgaggaatg ttatatgtcg gggcctcggc gtgtcgtcgt ggctcgaccg
     3241 ctccattctg acccacagcg ctatgccgag cttaccgagc aagcagaatg gctatcttca
     3301 acgttgttgg gagaaggtgc tcttgacgct gccttgatgc ggatcgcaat tttagcagcg
     3361 gatctaggtg aactcgacgc tgctgaagag attgcttcgc agatagttga ttctgaaatt
     3421 ttgggtaacg ttcttattga tatcgcttat gcggctgcat cgcagcaaga cgacgaaaga
     3481 atcgaacgta tttcgtcttg ggtcaaggag tttgatgatc gacctcatgc tatcgttcgt
     3541 ttgatagcga gggttgcgga gggattccgc tttgaacttg cagagtctct aatctcgtct
     3601 attgaagcgg ctgatataca agcctgtgga ctggcatgca ttggcctcgt gaagagccgt
     3661 gtagggcagc tagatgagtc tgtagagctt tttaggcgag gagagttgct tgcggagagt
     3721 cttgcctcga cgagccggcc taggcttata cgcttgctgg tcctcggtgc gaggtttata
     3781 ggcgaggacg ttgcgcgggc aattctggtg cgagcacttg ctcaagggtt gagcatgacg
     3841 tacatcgact gtgcattcga gttagattcg agtattgccc tgtctctcgg ggagaaattt
     3901 ggtgagggcc taagctggat ttctgttgac ccatccgccg cttagctccg cgggcaaata
     3961 cttattgctt ccacttgtgc ctcgcccccg ctgctcgatt gttcgtggcc cttggtcggt
     4021 gtcaacgccg tctgtgcggg ttgggggcgg gttgcggtgt acgtcgttgg tggacggaca
     4081 cgcgaagacg gcattgacac ctcggtcggg ccactgatgt cggcagctat cgggggcggg
     4141 ggagaagtgg tgcggggcgt agaaacaggt gcggccccgc ccgagtgagg ggggccgcgt
     4201 gagtgctgga ccgtttatcg catctcgccg agcagcaggg catgccacac cctgcgggcc
     4261 tcctcgaccg gccagcggtg tgtttcctgg acctttggtg tcggttcggc ctgggtggtc
     4321 ttgcggatga acgcctgccc gccggactgg agcagttcgt agacgacggc ccggcagccg
     4381 caggtccaag cgagagctcg cacgcgcccg accccctcga acggcttatg ccagacgagt
     4441 tctacgtgat ctttccggcg catctcccag tgcgggctca ggcactcgtc cgggtccagg
     4501 agggtggccg ccatcacagc cgccgcctta ctgtgccccg ccgttggccc agccgacgat
     4561 ccatcggacg gcggactgtg gctggtcgac cgagggcagc ggaacgacgg tcccggactt
     4621 cggtagcttg atcaggaagc ggcgcggctt gttctcctgg tcgatggtga ccgtcgcaac
     4681 ctgtccgccg aagccgtaga cgtccagctc cgcgcccgcc ttctcctgca ccgggaaccc
     4741 ggtcatccgc agggtgatgt ggctgatgaa gcgcgtccgg accccttggg atcggagtaa
     4801 gtcgccgagg gcggccagga tgcccgcctg ttggttggcg gtccggttgc cgtttgtgat
     4861 ctggcgcatg tctctcccgt ctgtctcgct gacgggttga gcgtacgcca accttcctac
     4921 tgcttcctag tccctcttat aggtacctct aggtacctgt ggtaacgaca tggaacgctt
     4981 tcgttcctac tcttgagccc gtgatcgagt tccggcctga ccagctcaaa tgggaagccg
     5041 tcctcgaaga gatacgacgg cgtatccgga cgggcgaata ccaggtgggc caccccatcc
     5101 ctggtgaacc tcgcatggcc gaagagttcg gcatcgccaa gggcaccgca cgccgcgtca
     5161 tcaacgaact gctcaaagcc ggagacgtct acaccgtgct cggcaagggc accttcgtcg
     5221 ccgaccccga gaccggcggc ccgcccagga gagacaccga agacgagtga aggcccccga
     5281 acgaatccgg gggccttctg ccttctatcg gttgtcagcg agacgacgga aggcgcacca
     5341 agcgtagagc ccacgggccg agggcagtag ctttccaccg tggcgccgcc ggcccaaccg
     5401 cttcgcggcc cttcgggtgc ctacggcatt gcgccggccg caccactccc gcacccacgc
     5461 ccggcaaggt cacgctggct ctccccgttc gcgggcgatt cggcgggcgg tggcgatgcg
     5521 ttgccggatc agctcggccc acaggtgttc gccgtgccgg tagccgcttc cagcgaagcg
     5581 ccactggccg accgtcaccg tctcccgctc atcacgagga ggcaggcccc gccgttcccg
     5641 agcccgctcg gcctggtgat ccgcgcgaat ctgacgcagc gcgccgagcg tggtcgagta
     5701 atggcggctc ttggtggaga agtggccccg atagccgagc atgtgcgccc actgcctcag
     5761 cgggatcagc cggtaggccg gatcgttcgc cagggcgaag cacgtgtaga tcatgcgccg
     5821 ggcatgctcg gtgacctcta ggcgcgcgac gtcggccatc tcccggatcg gccggtccac
     5881 ggtcccggct gactcggccc ccttcgtcgc gtacttcgcg atgtaggccg ccacccgctg
     5941 atccgcgatg ccctcaagcg aggccgcgac gtagatgggg tctacgtcga gttgcgttcc
     6001 ccaccggccg acgaacgacg gggcatcgtc cggcacggga accgagaccg cagccgcggt
     6061 ttcccgaatg gcggcctgga gcacgggcac ggtcgcccag tccggcggac gatcggacgg
     6121 cccgtccgga ccgtcgaggc ggatcaccgc atggaagtgg accaggcccc gcgcctgata
     6181 ctcggccacc ttggcgtacg acagccgcag ccgccgtcga agcgcggccc gtgagacgcc
     6241 gaggtgccgg gcgaaggccg ccggtagcgc ctgagtgaag cgccgccaca gctccccggc
     6301 atgggcgttc cacagcaccg cgccccggta gtcgtagcag tccacacaga tcggctgccc
     6361 gacccgtggg tcatcgtcgg catgccggac cccgcagcct tccggcctgc cgtgctcaca
     6421 caccgggttg tcccgccgag gacggcacgg caacaccttg tcacccttca tccggcgcgc
     6481 gtggaccgcg ccgaacgagg gggccgtgaa cgtggcgaac acgcggggat gcgcgctcac
     6541 gtcctcggga acgcccttgc cgccgcgcag gccggacagg atcaggtgat aggtgtcgtc
     6601 ccggtagacc tccgagcagg ccgggcaccg cgacgcacga cggttgccgc acgcggtcag
     6661 caggtagccc gtcggctcgt cggccgtccg gtagacgtcc agcacctcca acccggcatc
     6721 gaaagtgatc cggcgtccct gaagccggat cggctgggca cagccgcccg tcgcgcgcac
     6781 catctcctgc caccgggcga agtccggcat ggcggcgcgt tgggcggcgg ccagaacatc
     6841 gggggagagg ggagggagtt cgctcacgcg gcatccctcc cgctgtcgga ggcggtgtcg
     6901 tgcgcgtcgc cgacgtactc ggcaccctgg atggcggcga gccgccgcca cttgttgacg
     6961 ccccaccggg gcgagcgttc gagctgccgc ttgcaccact cctcagccag cgcagccatt
     7021 ggacccagtt cctcgggtac cagcggagac ctcctttcct cgtaggaagc cccctcgcgc
     7081 gaacccggga aagacaaagc accagcgcag agcagaccga aggcaggtct gtagtgcggt
     7141 ggtgctttca gcgaggcgat atgtggagtg tttatgcgac ggtgaaccgg tgagcacgtc
     7201 cagggggcct accgctgggc cgggtccctc cagcggaacg gcttgtgtgt tttgttgttt
     7261 gcccggtggt tcccctgtgc tcgaatttgt cgcggccggt agctcgctgt tgtgaatgtc
     7321 tttccgccct gtcgcggacg gagcccttgc cctttcagat gtaccacccg gagaacgtat
     7381 ttagccaggt cagcgttccg cgcatggccc ttttcgtgac cggccaccgt tgtttgctgg
     7441 cgttcactgg cgtccattgg cgttcgctga cgtatgtctg cgtagggagc aggggaaggc
     7501 agatgtcacg ccgaggtaag ggtgtcagtc ttcccgccag atgggttcga gcgcgttcgg
     7561 gtcgaagttg ccccacgacc ccatgccctt gcccacgggg tgaacgatca ccgcgtggaa
     7621 cgcctccatg accatcgcgc gcttggtgcc catggagagg ccgccctggt cctcgggcag
     7681 ataccagcgc cgccgtatgt cggccccgtc aacgatgcgc cgagcccgct tgcgctcctt
     7741 ggccgccgtg tacgcctggc cctcccgccg caactcgttg atccgctgct caagcgacgg
     7801 gacgagcatg aagaacagct cgttgctgat cgtgcgcgcg ttccactgcc gggtgagttc
     7861 ggcgagctgt tccttcttgt ctttcagctc ttgctcaccg gcccacggga cgtcctccgt
     7921 gtccgcgtcg ggggactcgt cgagcttcgc caggaccagc tcggatatga acacgtccac
     7981 cctgtcaccg cgccgcccga cgcccccgca tccgccgtcg ctcggggcag gacagacgta
     8041 gaggtgaact cctccccgtg ccggccgaac ccgtaggggc gcgttgcaga tgccggtccc
     8101 atcagccttg ggcttgccgc accggaggat gcccgacagg agatagcggt gctcccgctg
     8161 atcgaagcgc atgcccaggg ccgcgtactg atcggccttc tcccgtgagg tgcgctcgaa
     8221 ggcggcccgc acggcaagca attcatcggg tgtgatcagg gcttcccact tgcccatcac
     8281 cgggtcgccg tccgcgtcgc ggaccagctc accgttgatc tcgcgccacc cgcagagccg
     8341 gggactggac agggtggccc gcagggaacg gcgtgaccac tcgttccccc gcgtggtacg
     8401 caccccgagc tgctgccact tcatcacgat cgcgcgcagg gagcccccgg cgatgatggc
     8461 gtctaccgcc tcgcgcagca gcggcccctc ttcgggatga atggtgacgc gatcggccag
     8521 ccagccgaac ggacgagggc cgcccaccgg tacgccctgc tcggcccgcg cccggtgaga
     8581 gcgacgcata cgccgctgca tcttccggac ttccatcttc gagatcacgg ccccgaacag
     8641 gcccatgctc tcggtgtcct cgctgtagag gtccttcggc ccgcgcgcgt cggcgaagac
     8701 ccgcccgtct cgataggtga aggcttccac gaagcgctcg tagtcgccgg gacggcgtgc
     8761 cagccggtcc tcagcgacga tcaccacacc ttctataagt gtcccgtccg cgaggcgtcc
     8821 ggcccggagc gccttgacca tcgcctcgaa ggcgtcgcgt acgacgttgg ccttggcggc
     8881 ggacttgtcg ttgtcggtga actcgtggac gatcgtccag ccgagccgcc cggccgtctg
     8941 tcggttgacc ttgtgctgat cctgtacgcc gtgctcgtct ccccgtccgt ccgacgagat
     9001 gcgcgcgtac gagacgacag ggacaacctc tggcatgggg gcctcctcga agtttgcact
     9061 ccaaggaagg ttctaaccat agatgcccca ctggatgaag taggccaccc gcttgtagcg
     9121 gtctccgccg ccggtggtgg cctgggccgc tgacggcgcg agcccggccg ccagggtggc
     9181 ggcgaccgcc gcggcgaggg tccttctcat gagcacgccg gcctccgagc gctttttata
     9241 ggaaagtttc ctgtaaattg tcgcgatcgt agaccccggt gatcagggcg tcaatggcag
     9301 cgcccgggtg acggcggagg ggagaccggg ctggaggccg gcgccgcgcg gcgcgaccgc
     9361 gccgccacgg cggcatccgc acccccgtcc gggcgctgcc gggcgagcgg tgacggatca
     9421 aggatgcatg agaagcagcg ggtcgcaggt gtcggttccg accagatggg ggttgttcgg
     9481 gtccatcaaa gccttctcgc tgttgatgat gtaaaagcct ccggcggccc ggggtatcac
     9541 gatcagcgtt tgatactgca tgagcgcctg agccttggcg gtggttcctc catgtccaag
     9601 agcgtgcttc agcatggtcc tgagcatggc gaagagattg tcgtggtgga atcccggcca
     9661 gatgagcact cggtaggtgt gcgggatgat cccgagattc tgactgagac cccggatcga
     9721 cacgcgcgag tccgcccagt gcgccacggt tctggcggcc aggttgtcct gggcggcgag
     9781 gtcgtgctgg gactccaatc gcgcccgtgc catatgaatg tgcagctggt tgaaacctct
     9841 ggcctgcttc gagttgatgc ccaatcccac cggggtggga accacagtgg gaggcttggt
     9901 ggcatagtag ttcgcggcgc tccaataatt cggtgcactg ctgccgcaga tccaggaaca
     9961 ctcgatgcca ctgatccgct gcgtggggac gagaatgtag ttgacgtact tcttcttgct
    10021 cttggtgccg cccatcacca cgtagtcggc atggttctgg cggcaggtgt tgttgctgtg
    10081 acacttctgg ctgagccgcc agagggagtc ggagtccttg gggcttccgc actccttggg
    10141 attaggccct ttggcgagtg ccgatgaagg cgcggccagc gcgtgctcgg caccggaaag
    10201 aatgcccact gccagcatgc tggtgcccgc caggccggag aactggatga accggcgtcg
    10261 cgtcagctcg
//
