LOCUS       NZ_JACJHT010000002     17426 bp    DNA     linear   CON 19-SEP-2024
DEFINITION  Priestia aryabhattai strain S00060 Ga0436288_02, whole genome
            shotgun sequence.
ACCESSION   NZ_JACJHT010000002
VERSION     NZ_JACJHT010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Priestia aryabhattai
  ORGANISM  Priestia aryabhattai
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 1150867)
  AUTHORS   Carlos-Shanley,C.
  TITLE     Functional genomics of gut bacteria from endangered species of
            beetles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1150867)
  AUTHORS   Carlos-Shanley,C., Huntemann,M., Clum,A., Wang,J., Palaniappan,K.,
            Ritter,S., Chen,I.-M., Stamatis,D., Reddy,T., O'Malley,R., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2020) Prokaryote Super Program, DOE Joint Genome
            Institute, 1 Cyclotron Road, Mail Stop: 91R0183, Berkeley, CA 94720,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.14.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 248.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014138775.1-RS_2024_09_19
            Annotation Date                   :: 09/19/2024 05:54:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,314
            CDSs (total)                      :: 6,206
            Genes (coding)                    :: 6,105
            CDSs (with protein)               :: 6,105
            Genes (RNA)                       :: 108
            rRNAs                             :: 8, 5, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 4, 5, 3 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 101
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 32 of 103
            Pseudo Genes (incomplete)         :: 62 of 103
            Pseudo Genes (internal stop)      :: 19 of 103
            Pseudo Genes (multiple problems)  :: 12 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:01:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 164878
            Orig. end                         :: 182304
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JACJHT010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17426
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="2"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..17426
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17426
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:12426]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12426
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            complement(40..714)
                     /locus_tag="HNP21_RS10260"
                     /old_locus_tag="HNP21_002136"
     CDS             complement(40..714)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081817.1"
                     /locus_tag="HNP21_RS10260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002136"
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="WP_150368979.1"
                     /transl_table=11
                     /translation="MTLKFIHGNYLKYNKGTGFISCHVDALHAEPPAADLFTDKVVENL
                     VKKTGCAGIVSTVSRTIADLNRNLNETNHEGIHEYRRTIQEIVKYLKIADSNNRLLTPY
                     LHLSFHGMKDTHYGPYAIEVGTLYGQSCSKEVKDWFQQALTKKSEDSFPKIHVIFDNKF
                     VGNESIAFHRQGDNSGYLGYSHNFNTFQIELSRTLRSKHRTTITNIFSEIIDDFQQTFV
                     AT"
     gene            960..3581
                     /locus_tag="HNP21_RS10265"
                     /old_locus_tag="HNP21_002137"
     CDS             960..3581
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081818.1"
                     /locus_tag="HNP21_RS10265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002137"
                     /product="YheC/YheD family protein"
                     /protein_id="WP_182527513.1"
                     /transl_table=11
                     /translation="MDPMREELGILSDKEMTLQTLNLNNIPSVELVDPKTCSYPVIGRK
                     YGHHSGRDIVIVNTKDQAIYEGYDYFTKIYVIDKEYCLEVEGLSVKTVQVVTSEHVVFN
                     EIPIRTQAFGWKLEQVNGMDVPEMLMNVAIRALYVTGAKSGFVKMGVLENGECIVTDIN
                     SSESEWIENPLKPSVPFSMGADVEFMLSCDGELLPASTFFSVEGPVGCDERQIEQDSGE
                     YALVEVRPEKANSSTELFENIQKLIEKASAQVPYENIHFRAGSMPFSGYQCGGHIHFGI
                     PLSLSLLRALDHYLAIPVALIEESKTAKLRRKTNHGGLGRYREKPYGFEYLTLSSWIID
                     PRITLSTLALAQLVATHHHELKSEFLFHPLTQRAYYQGNKIFLKRMWKDIKANLMKTSS
                     YPHYQNELSFLFEMIEKEIPCDESNDIRRNWNVKISKEIYDRGHIIQIPKKLRLKYGLK
                     EGQSTMVSAGKAISTATVHSYPFSFRHPNMVQLSKSLRDKLSLPKDWCPKLSASEGIIT
                     LGPIIGILANRPFERQTTYFHHLCRLANEKRMLVYVFEPEDIDWEKKLVKGTTINGEGL
                     FPFPAVIYDRYFIDSRKNILIDEVRAKLQAIYKIPFVNSSNLFQLTGDKWATYELLMKE
                     YEEFLPESRLVQKSADIAEMLDSYGEVYLKPLGGALSKGVMRIVRRPTGIFWFDLNKKE
                     LHQFSNMEELFTLLSPLMKNNPYLVQEGIRRKQHKDKNLEIRVYMQKNEKQIWLRTGMV
                     ARLTGEDVLTEDSETNMRLSKILNSLYPDPTDRRLIINQLAKISKNIVATVEEKVGPFG
                     ELAVDLCIDQYGSIKLLEINAKPDSLFSQIRAYKLRTLAGIRLLNYASSLAGYEEEKED
                     LT"
     gene            3578..4987
                     /locus_tag="HNP21_RS10270"
                     /old_locus_tag="HNP21_002138"
     CDS             3578..4987
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055491.1"
                     /locus_tag="HNP21_RS10270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002138"
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_182527514.1"
                     /transl_table=11
                     /translation="MKHFTLNKILKQTGGDLCYGSGNPVIKHAINYAKKDIEDHTLIFH
                     LDREPIRGKYWKENHSIVVITDHPELCTNLEGNIILVKTAQLEEAYWKFIEYYRGLFDL
                     PVIGVTGTCGKTTTKEMIRQILIEDYKVKATWMSMNSMSVNLRYLTGIDDTTEVAVFEM
                     PVAYPGYLKVACRYFQPQIRILLNIGVHHLADCETPEEYMKAKAEIVEGLDPNSGILIL
                     NADDENIKRVLDVSHLQGVVYIGKGDDSQFQAKDIKYAKGGMAFTLKYQNKEYDAFIPG
                     YGEHNVYNALAAIAAVFYVGVDISVAIQRLSLFEQVEEHLEFKRGLNECTVINDTWNSS
                     PLSMATALGVLSDMSKEKQSIALLGYMPQLGEGPYAIKQYEDMGKKAAESKIDILVVVG
                     DEAKAIGTGALKCGMDPNKVYFCKTGDEVYNVLAPYLNQNTIILLKVTHRVMTKPTFKE
                     LRNKLIPDNEE"
     gene            5001..6518
                     /gene="aepX"
                     /locus_tag="HNP21_RS10275"
                     /old_locus_tag="HNP21_002139"
     CDS             5001..6518
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055492.1"
                     /locus_tag="HNP21_RS10275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002139"
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_013055492.1"
                     /sec_met_domain="phosphonates-like (E-value: 5.1e-138,
                     bitscore: 454.7, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 1.3e-126, bitscore:
                     417.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1.6e-63, bitscore:
                     210.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKTKQLRELIESKQLEFIMEAHSALSAKIVEEAGFKGIWASGLS
                     LSAALGVRDNNEASWTQVLDILEFMSDATSVPILLDGDTGYGNFNNARRLVKKLEQRDI
                     PGVCIEDKLFPKTNSFIKGEAQPLADINEFCGKIKAMKDSQNDEDFVVVSRVEAFIAGW
                     GLKEALKRAEAYRQAGTDAVLIHSKRSDMTEIEAFMKEWGNRLPVIIVPTKYYSVPTDK
                     FSDLGISLSIWANHNLRSSITAMQKTSAQIYCDKSLINVEKNVSPLEEVFRLQKAEELD
                     MAEKLYLSSANHSTNAMILVKESANEALITEQINQLKKVGVTNSIKAGSNKAMEDKAFP
                     NKGELYHLYAARDKLGTATLISDSNIVYKTHVFEELIHESGDITIVAGADYELKGNQTS
                     YVTAKLPYSKQLYSKTVDLIQMSCNPNSNNIHGEFIGLWKVSGKGTEVVKKALEELAQR
                     KDFAQLTFSDFFNYINLIHPVAVKYTMGSWIDTETYTKIQKDGGKND"
     gene            6511..7641
                     /gene="aepY"
                     /locus_tag="HNP21_RS10280"
                     /old_locus_tag="HNP21_002140"
     CDS             6511..7641
                     /EC_number="4.1.1.82"
                     /GO_function="GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0032923 - organic phosphonate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081821.1"
                     /locus_tag="HNP21_RS10280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002140"
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="WP_013055493.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.3e-09, bitscore:
                     33.9, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 6.5e-14, bitscore:
                     48.1, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISTSVFGTQLKKLGFQFYSGVPCSYLKHLINYAINECEYVAAAN
                     EGEAVAIAAGASIAGNKAVVLMQNSGLANAVSPLTSLIHPFKIPVLGFVSFRGEPGCPD
                     EPQHELMGEITPALLNTMKIKWEYLSFDFAEVKKQLQRANQAVENNESYFFIVKKGTFE
                     PVKLLEKSIKNVTPKMKSEQITTDELPSRYEALQVVNSLKDNDTIQLAATGKTGRELYE
                     IEDARNNFYMVGSMGCVSSIGLGLALSRNEKNIITIDGDGALLMRMGNLATNGYYCPAN
                     LLHILLDNQTHDSTGGQHTVSHNINFVDIASSCGYQNSIHVHNLYELETYIKQWKSDKK
                     LTFLYLKIAKGSKKNLGRPEMKPYEVRERLQVFLDD"
     gene            7634..9475
                     /locus_tag="HNP21_RS10285"
                     /old_locus_tag="HNP21_002141"
     CDS             7634..9475
                     /EC_number="2.6.1.37"
                     /GO_function="GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (266-542). E-value: 6.8e-30.
                     Score: 96.1. Matches aSDomain:
                     nrpspksdomains_HNP21_RS10285_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055494.1"
                     /locus_tag="HNP21_RS10285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002141"
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="WP_053486853.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.6e-11, bitscore:
                     40.8, seeds: 297, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 9.5e-25, bitscore:
                     83.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKTAVILAAGMGSRIRKRAGNRPKGFLMIDEKSIIEHSISKLIE
                     AGVKTIFIGTGYMKEEYEKLMVRYPQIKCVYNSRYETTGSLFTLYQFKNYIKEDFLLLE
                     SDVIYEKNALETLVNHSRSDVILASKLNGAGDEVYIEADEDNNLKNMSKQKEELNSIYA
                     ELVGLTKLSYATFQRLCDEANTLFQFNQTIDYEYGLVKISEKANVYVHKLDNLAWCEVD
                     DEDHWARATTIVYPIIKAREGIPHTVKRNILLNPGPATTTDTVKYAQIVEDICPREKQF
                     GETMEFISAELTKFVGNPEKYTTVLFGGSGTAAVESILSSVIDNGTVVIINNGPYGERM
                     CKIAEIYGLNYLEYKSSAEQAIDMNDLKIFIKKISTNVSYLALVHCETSTGLLNDIEQI
                     GEFCAVKNIEMIVDAMSSYAAVPIDMQKMNISYLAASANKNLQGMAGVSFVIANKDRLE
                     KTEQIKPRNLYLHLYDQYRYFQMNKQMRFTPPVQTMYALKQAIIETQWEGIEKRYERYS
                     KSWTTLTDGITRLGLTYLVPETHHSKIITSIIEPSNRKYNFDIMHDFFYKQGFTIYPGK
                     IDKLETFRIANIGDITYRDIERFLHLLEQYLKALKGE"
     aSDomain        8432..9259
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HNP21_RS10285_Aminotran_5.1"
                     /evalue="6.80E-30"
                     /label="HNP21_RS10285_Aminotran_5.1"
                     /locus_tag="HNP21_RS10285"
                     /protein_end="542"
                     /protein_start="266"
                     /score="96.1"
                     /tool="antismash"
                     /translation="YAQIVEDICPREKQFGETMEFISAELTKFVGNPEKYTTVLFGGSG
                     TAAVESILSSVIDNGTVVIINNGPYGERMCKIAEIYGLNYLEYKSSAEQAIDMNDLKIF
                     IKKISTNVSYLALVHCETSTGLLNDIEQIGEFCAVKNIEMIVDAMSSYAAVPIDMQKMN
                     ISYLAASANKNLQGMAGVSFVIANKDRLEKTEQIKPRNLYLHLYDQYRYFQMNKQMRFT
                     PPVQTMYALKQAIIETQWEGIEKRYERYSKSWTTLTDGITRLGLTYLVPETHHS"
     gene            9483..9905
                     /locus_tag="HNP21_RS10290"
                     /old_locus_tag="HNP21_002142"
     CDS             9483..9905
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461506.1"
                     /locus_tag="HNP21_RS10290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002142"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_013055495.1"
                     /transl_table=11
                     /translation="MNQKLAVAVMVVNQDHHVLLVKNHRRGWEFPGGFVEAGESIKDAG
                     IREVKEESGIVIEVTNFLGVEQDVKRSTTVILLKGKAISGKISVSNETQDVGYFTLEEA
                     KNIIKLDNFKDRMARCLKETEIPFFYVLKDTGSFYV"
     gene            complement(10017..10730)
                     /gene="budA"
                     /locus_tag="HNP21_RS10295"
                     /old_locus_tag="HNP21_002143"
     CDS             complement(10017..10730)
                     /EC_number="4.1.1.5"
                     /GO_function="GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="budA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763108.1"
                     /locus_tag="HNP21_RS10295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002143"
                     /product="acetolactate decarboxylase"
                     /protein_id="WP_013055496.1"
                     /transl_table=11
                     /translation="MSNKEKLTIHQTSTMIALLDGVFDGVVTYKDLAKKGDFGIGTFNQ
                     LDGEMIAFDGEFYHIADGKAAPISEDAKTPFAIMTTFYEDISYRVEKEMNREQLETLMN
                     ELIPSPNLFYAVRIDGKFKEVKTRTVAKQEKPYPSMVDAAADQPTFTSSDVNGTLAGFY
                     TPEYSSGIGVPGYHLHFIDEYKKEGGHVLDVSVQDVTIQICKKTKLNLNLSETSEFLSA
                     NLEDHDVEEEIATAE"
     gene            complement(10747..12426)
                     /gene="alsS"
                     /locus_tag="HNP21_RS10300"
                     /old_locus_tag="HNP21_002144"
     CDS             complement(10747..12426)
                     /EC_number="2.2.1.6"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003984 - acetolactate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="alsS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081825.1"
                     /locus_tag="HNP21_RS10300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002144"
                     /product="acetolactate synthase AlsS"
                     /protein_id="WP_150368987.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.5e-51, bitscore:
                     170.5, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.2e-45, bitscore:
                     150.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMSNADTTETKKGADLLVESLASQNVPYIFGIPGAKIDGVFDVLK
                     EKGPEVIVCRHEQNAAFMAAAMGRLTGTPGVCLVTSGPGAANLATGLVTATSEGDPVVA
                     LAGAVPRADRLKKTHQSMDNEGLFKSIAKFSVEVEDAANVPEVITDAFREAVSSPSGAS
                     FVSLPQDVMDNETSASTLSVLKAPTIGAANLDAVAQAIHTIRTAKLPVLLLGMRASKSK
                     EVESIRQLIAAHDLPVVETFQGAGAVSRELETHFFGRVGLFRNQPGDVLLKHADVVLTI
                     GYDPVEYPPSLWNSESQRSIIHLDDLQAQADHHYQPEIELVGDIPSTIDILADKLPHLI
                     RSKESASFLKTLKSKLEQPDDQYEFSNDTHLHPLQLIEALQNFVEDDTTITCDVGSHYI
                     WMARYFRSYEPQHLLFSNGMQTLGVALPWAISAALVRPHKKVLSMSGDGGFLFSAMELE
                     TAVRLNLPIVHIIWNDQTYDMVGFQQQIKYGRKSAVEFGSVDFVMLAESFGAKGFRVNS
                     PEEFSDTLASAFREQGPVIIDVPVDYKDNEKLNTQLLPDQIN"
     misc_feature    complement(12523..12537)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.86"
                     /tool="antismash"
     gene            complement(12652..12816)
                     /locus_tag="HNP21_RS10305"
                     /old_locus_tag="HNP21_002145"
     CDS             complement(12652..12816)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461503.1"
                     /locus_tag="HNP21_RS10305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002145"
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_078081481.1"
                     /transl_table=11
                     /translation="MKQPQLEKEIRALQSDIYQLAKKTSSYSHGEILKLSQKLDQKIVS
                     YQKLFNRTK"
     gene            complement(13687..13884)
                     /locus_tag="HNP21_RS10310"
                     /old_locus_tag="HNP21_002146"
     CDS             complement(13687..13884)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055498.1"
                     /locus_tag="HNP21_RS10310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002146"
                     /product="YwbE family protein"
                     /protein_id="WP_013055498.1"
                     /transl_table=11
                     /translation="MNGQNRKDVAPGIEVDIVLKADQRSGKLTHGTVKDILTNSSFHPH
                     GIKVRLTDGQVGRIKEIYPN"
     gene            complement(13884..14063)
                     /locus_tag="HNP21_RS10315"
                     /old_locus_tag="HNP21_002147"
     CDS             complement(13884..14063)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055499.1"
                     /locus_tag="HNP21_RS10315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002147"
                     /product="hypothetical protein"
                     /protein_id="WP_013055499.1"
                     /transl_table=11
                     /translation="MTEQLKKKISLQDAIKQQLANKKNQSSSKAQNSSTNQATKKLTSQ
                     QTKKTNNQRKRTGV"
     misc_feature    complement(14126..14141)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            complement(14148..14516)
                     /locus_tag="HNP21_RS10320"
                     /old_locus_tag="HNP21_002148"
     CDS             complement(14148..14516)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461499.1"
                     /locus_tag="HNP21_RS10320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002148"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_013055500.1"
                     /transl_table=11
                     /translation="MKKYNIPVEAALEVIGGKWKVVILCHLIKGTKRPGELKRIMPRVT
                     QKVLTQQLRELEADGIIVRKMYNEMPPRVEYSLTDYGWSLKSILDSLCSWGEHHIEKTY
                     PDKEAVLMQPVEEVTVSN"
     gene            14846..16045
                     /locus_tag="HNP21_RS10325"
                     /old_locus_tag="HNP21_002149"
     CDS             14846..16045
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461498.1"
                     /locus_tag="HNP21_RS10325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002149"
                     /product="MFS transporter"
                     /protein_id="WP_053486854.1"
                     /transl_table=11
                     /translation="MNTLSQTQQSKKSGALPLLALAISAFGIGTTEFVPVGLLSSLADD
                     LNISITLAGLLISGYAMGVAFGAPVLTALTSKMSRKSLLMLLMVIFIVGNSVAALSTSF
                     GLLLIARIITAFSHGIFFSIGATIAADLVPEDKRASAIALMFTGLTVATVTGVPLGTFI
                     GQAFGWRATFWGVALLGVIAIIASALLVPKNLKEAPPAKFSDQLKILRNGPLLLSFAIT
                     ALGYGGTFVAYTYLAPILEKLTGYAPSAVSILLLVYGVAVAIGNTIGGKAANKNPLKAL
                     FWMFLIQAVILVILSFTASFKVAGTITIFAMGLLAFMNVPGLQVLVVNLAEKYVPSAVN
                     VASALNIAAFNVGIAIGSFVGGLIVDSIGLVHTPWIGGVMVLGAVLLTAWSRSIEAKAK
                     "
     gene            16103..17059
                     /locus_tag="HNP21_RS10330"
                     /old_locus_tag="HNP21_002150"
     CDS             16103..17059
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081827.1"
                     /locus_tag="HNP21_RS10330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HNP21_002150"
                     /product="LLM class oxidoreductase"
                     /protein_id="WP_182527515.1"
                     /transl_table=11
                     /translation="MTTFQHHKAYRRMYKKNELTLGLHMPLENYQYATPTMEKQVELTQ
                     LAEQYEFTSLWFRDVLLQDPSFGDPATGQIYDMLIYLTYLASQTKEIALSTAAIVLPLR
                     HPLRVAKEMATIDQLFPERLIMGVSSGDRQADFRGLGIPHEERGKRFAEAFHYLEEALN
                     KEFPSIQSALGSIQGANVVPKPTKRIPTMITGYSQQNMEWFAQYGDGWMYYPRHPHFQA
                     EAITQWRKLVDIYHPGVFKPFTQPMHLDLAEDPNEMPTPIRLGFRAGRNALVELLSIYK
                     EIGVNHLFFALFDSKRPAEEVIHELGEEVLPHFPSHM"
ORIGIN
        1 tatataaaag aaatagtaga cggaaaagat tcctaaaaac taagtagcga cgaatgtttg
       61 ttgaaagtca tcaataatct cggaaaatat atttgttata gtagttctgt gtttgctgcg
      121 cagggtccgg gataattcaa tttgaaatgt attaaaatta tgactgtaac ctaaatagcc
      181 gctgttgtcg ccttgtctgt gaaaggcaat tgattcgttt ccaacaaatt tattatcaaa
      241 tattacatgg attttcggga acgaatcttc agatttttta gtcagcgcct gttgaaacca
      301 atctttaact tcctttgagc aagattgtcc atataaagtc ccgacttcaa ttgcataagg
      361 tccgtaatgt gtatccttca ttccatgaaa agataaatgc aaatagggcg ttagaaggcg
      421 attattacta tctgctattt ttaaatattt aacaatttct tggattgttc ttctatattc
      481 atgtatcccc tcatggtttg tttcatttag atttctattt aaatctgcta tggttctgga
      541 aaccgtacta acgattcctg cacatcctgt tttcttgact aaattttcta caactttatc
      601 cgtaaataaa tcagcagcag gtggttctgc atgaagtgca tccacatgac agcttataaa
      661 accagttcct ttgttgtatt ttaagtagtt tccatgaatg aattttaatg tcattgacta
      721 tttctccttt tatatcgttt gaaatctatc ctttctataa taaatgcagt aaaaataaaa
      781 tcggtgaatt attacacgtg ttaaatacat acataaaaag tttttagaga tggtggagat
      841 aaatagataa atgtacagga ttattttgaa tagttacata ctatatgagt agctgattat
      901 tgttataggg cattaaagta gcaaaatacg atagaaagtt ctaattagga ggctttaaaa
      961 tggatccaat gagagaagag ttagggatat tatctgataa agaaatgacg ttgcaaactc
     1021 ttaatctgaa taatataccg tctgttgaat tagttgaccc caaaacctgt tcatatccag
     1081 tgatagggag gaagtatggt caccatagcg gaagagatat tgttatcgta aatacaaagg
     1141 atcaagcaat atatgaagga tatgactatt ttacgaaaat atatgtaata gacaaagaat
     1201 attgtctaga agtagaagga ctgagcgtaa aaacagtaca agtagtgacc agtgaacatg
     1261 ttgtatttaa tgaaataccc attcgaacgc aagccttcgg ctggaaatta gaacaggtaa
     1321 acggcatgga tgtaccggaa atgctgatga acgtagccat tcgagcgctc tacgtaacgg
     1381 gagctaaaag cggctttgtc aaaatggggg tattagaaaa tggagagtgt atcgtcacag
     1441 atattaactc atcagaaagt gaatggatag aaaatccatt aaagcctagc gttcctttta
     1501 gcatgggtgc tgacgttgag tttatgttaa gttgtgatgg agagcttcta ccagcttcta
     1561 cttttttttc tgttgaaggt ccggtaggct gtgatgaaag acaaatagag caagatagcg
     1621 gtgagtatgc attagtagaa gttaggcctg aaaaagcaaa ttcatcaaca gaactatttg
     1681 agaatatcca aaagttaata gaaaaagcat ctgcacaggt accatatgaa aatattcatt
     1741 ttcgggcagg cagcatgccg ttttcagggt atcaatgtgg aggacatatt cattttggta
     1801 ttccgctttc tttatcctta ttaagagctc ttgaccacta ccttgctatt ccagtagcgc
     1861 tgatagaaga atctaagacc gctaaactca gacggaaaac gaaccatgga ggtcttggac
     1921 gctatcgtga gaaaccgtat ggatttgagt atttaacttt aagctcttgg attatcgacc
     1981 ctagaattac tctctccact ttagctcttg ctcagctagt tgctacccat catcatgaat
     2041 taaaaagcga atttttattt catccactta cgcagcgtgc ctactatcaa gggaataaaa
     2101 tctttcttaa acgaatgtgg aaagatatca aagccaatct tatgaaaaca tccagctatc
     2161 cacactatca aaatgaacta tcttttctat ttgaaatgat agaaaaagaa attccttgtg
     2221 atgaatcaaa tgatattcgc cggaattgga atgtaaagat ttctaaagaa atctatgacc
     2281 gcggacatat tatccaaatc cccaaaaagc tccgtttaaa atacggcctc aaagaggggc
     2341 agtctacgat ggttagtgca ggaaaggcaa tatcaactgc aactgttcat tcttatcctt
     2401 tttcatttcg ccatccaaat atggttcaac tctctaaatc tctccgtgat aaactatctc
     2461 tcccgaaaga ttggtgcccc aagctatcag cttcagaagg catcataact ttaggtccta
     2521 tcattggcat tcttgctaac cgtccttttg aaagacaaac aacctatttc catcatctat
     2581 gtcgcttagc aaatgaaaag cgaatgcttg tatatgtgtt tgaacccgaa gatattgatt
     2641 gggagaaaaa attggttaaa ggaacaacca ttaacggtga agggcttttt ccttttcctg
     2701 ctgttatcta tgaccgttat ttcatagata gtagaaaaaa tattttaata gatgaagtaa
     2761 gggcaaaact tcaggcgatt tataaaattc cttttgttaa ttcttcaaat ttgtttcagc
     2821 tcacgggaga taagtgggca acatatgaac ttcttatgaa agaatatgaa gaatttcttc
     2881 cagagtcacg tctcgtacaa aaatctgctg acattgcaga aatgcttgat agctacggag
     2941 aagtctattt aaaaccgctt ggcggggcct taagtaaagg agtcatgcgt atcgttcgcc
     3001 gaccaacagg catcttttgg tttgatctta ataaaaaaga gctgcatcaa ttcagtaata
     3061 tggaagaatt atttactttg ctctctcctt tgatgaaaaa caatccttat cttgttcaag
     3121 aaggaattcg cagaaaacag cataaagata aaaatttaga aatacgggtt tatatgcaaa
     3181 aaaatgaaaa gcaaatttgg cttcgaacgg gtatggttgc gcgtctcact ggtgaagatg
     3241 tgctaacgga agattctgaa acgaatatgc gcctcagtaa aatattaaac agtttatatc
     3301 ccgatccaac agacagaagg ctcattataa accaattagc taaaatatct aaaaatattg
     3361 ttgcaacagt agaggagaaa gtgggtcctt ttggggagct ggcggtagat ttatgtatag
     3421 atcagtacgg ttccataaag ttacttgaaa taaatgcaaa accagatagt ctattctcac
     3481 aaattagagc ctacaagctg cgtactttgg cggggattcg cctgctcaat tatgcgtctt
     3541 cactggctgg ctacgaagaa gaaaaggagg atttaacatg aagcacttca cattaaataa
     3601 aatccttaaa caaacggggg gagacctttg ttatggatca ggaaatcctg ttattaaaca
     3661 tgctataaac tacgctaaaa aggatataga agatcataca ctcatctttc atttagatag
     3721 agaaccgatt agagggaagt attggaaaga aaatcattca attgtcgtta taacagatca
     3781 ccctgagctg tgcacgaact tagaggggaa tattattctt gttaaaacag ctcaattaga
     3841 agaggcttat tggaaattta tcgaatacta ccgagggctt tttgatcttc ctgttattgg
     3901 tgttacagga acttgtggaa aaacgaccac gaaagagatg atccgacaaa tattaattga
     3961 agattataaa gtgaaagcta cgtggatgag tatgaattct atgtctgtaa atctacgtta
     4021 tttaacaggt attgatgata cgacagaagt agctgttttt gaaatgccgg tcgcttatcc
     4081 gggctattta aaagttgcgt gtcgctactt tcagccacaa atacgaattc tattaaatat
     4141 aggcgtgcac catctcgctg attgtgaaac acctgaagaa tatatgaaag caaaagccga
     4201 aattgtagaa ggcttagatc caaatagcgg cattttgatt ttaaacgcag atgatgaaaa
     4261 tattaagaga gtgcttgatg ttagtcatct tcaaggtgtt gtatatatag gtaaaggtga
     4321 tgattcccaa tttcaagcaa aagatattaa atatgctaag ggaggaatgg ctttcacttt
     4381 aaagtaccaa aacaaagagt acgatgcctt tatccctgga tatggtgaac ataatgtgta
     4441 taatgcactg gctgccattg ctgcagtatt ttatgtggga gttgatattt ccgttgccat
     4501 acaaagactt agcttatttg aacaagtaga agaacatttg gaatttaaaa gaggactgaa
     4561 tgaatgtacg gtaattaacg acacatggaa ctcttcacct ttatcgatgg ctacagctct
     4621 tggagttctt agtgatatgt caaaagagaa acagtctatc gctttattag gctacatgcc
     4681 tcaactagga gaaggaccat atgcaattaa acaatatgaa gatatgggaa aaaaagcagc
     4741 ggaatccaaa attgacattc tagttgtagt aggagatgaa gcaaaagcta ttggaacagg
     4801 agctttaaaa tgtggaatgg accctaataa agtctatttt tgcaagactg gtgatgaagt
     4861 atacaatgtg ctagctccat atttaaatca aaatacaata attttattaa aagtaacgca
     4921 ccgtgttatg acaaaaccta cttttaaaga attaagaaat aaacttattc cagacaacga
     4981 agagtaaaag gaggatagac atgaagaaga ctaaacagct aagagagttg atagaaagta
     5041 aacagctaga gtttattatg gaggctcata gtgcgctatc tgcaaagatt gtagaagaag
     5101 cagggttcaa agggatatgg gccagcggcc tgtcattatc tgcagcactg ggtgtaagag
     5161 ataataacga ggcttcatgg acacaggtgt tagacatctt agagtttatg agtgacgcaa
     5221 cgtccgttcc aattcttctt gatggtgata caggctatgg gaactttaat aatgcaagaa
     5281 gattagttaa gaaattggag cagcgagaca ttccaggcgt atgtattgaa gataaattgt
     5341 ttccaaaaac aaactcattt attaaaggtg aagcacagcc gcttgcagat attaatgaat
     5401 tttgcggcaa aattaaagca atgaaagata gccaaaatga cgaagatttt gtagtggtat
     5461 cgcgtgtaga agcttttatt gcagggtggg ggctgaagga agctttaaag cgtgcagaag
     5521 cttatcgcca agcaggaacc gacgccgttt tgattcatag taaaaggtca gatatgacag
     5581 agattgaagc ttttatgaaa gagtggggaa acaggcttcc ggttattatt gttccaacca
     5641 aatattattc ggttccaact gataaattta gtgatttagg aattagcttg agcatttggg
     5701 ccaaccataa cttaagatct tccattacag ctatgcaaaa aacgtccgct cagatttatt
     5761 gcgataaaag tttgattaat gtagaaaaaa atgtctctcc gcttgaggaa gttttccgtc
     5821 ttcaaaaagc tgaagagctc gatatggcag agaaacttta tttaagctct gccaatcata
     5881 gcacgaacgc tatgattctg gtaaaagaat cagcaaatga agctttgata accgagcaga
     5941 taaatcagtt aaaaaaagtc ggcgtgacaa atagtataaa agcaggttcc aacaaagcta
     6001 tggaagataa ggcgtttcct aataaaggag aattatatca tctctatgct gcaagagata
     6061 agctaggaac ggctacttta ataagcgata gtaacatcgt atataaaaca catgtttttg
     6121 aggagttaat tcatgaaagt ggcgatatca ctatcgtagc aggagctgat tatgagttaa
     6181 aagggaatca aacatcttat gtaactgcta aacttcccta ttccaagcag ctgtattcta
     6241 aaacagtaga cttgattcaa atgtcgtgca atccaaactc taacaacatt catggtgaat
     6301 tcattggtct ttggaaagtt tctggaaaag gcactgaggt tgtaaaaaaa gctttggaag
     6361 aactggcaca aagaaaagat tttgctcagc tcacgttttc tgatttcttt aattatatta
     6421 atctaattca cccagttgct gttaaatata cgatggggtc atggatagat accgaaacgt
     6481 atacaaagat tcaaaaagat ggtgggaaaa atgattagta cgtccgtatt tggaactcaa
     6541 ttaaaaaaac taggttttca attttatagc ggggttcctt gttcctatct taaacatctt
     6601 attaactacg ctatcaacga atgtgaatat gttgcagcag caaacgaagg agaagcggta
     6661 gcgattgctg ctggggcatc aattgctggc aacaaagcgg ttgttttaat gcagaactcc
     6721 ggattagcaa atgctgtttc ccctttaacc tctcttattc atccgtttaa aattcctgtc
     6781 cttggatttg tcagttttcg cggagaacct ggatgtccag atgaaccaca gcatgaatta
     6841 atgggagaaa ttactcctgc cctactaaat acgatgaaaa taaaatggga atatttatct
     6901 tttgattttg cggaagttaa aaaacaatta cagcgggcta atcaagctgt tgaaaataat
     6961 gaaagctact tctttattgt aaaaaaagga acgtttgagc cagttaagct tttagaaaaa
     7021 tcaataaaaa acgttactcc taaaatgaaa agtgaacaga taacaaccga cgagttacct
     7081 tcaaggtatg aggcattaca agtagttaat tcattaaaag acaacgatac tattcaattg
     7141 gccgctacag gaaaaacagg aagagagctt tacgaaatag aagacgcaag aaataatttt
     7201 tatatggttg gatctatggg gtgcgttagt tccattggtt taggcttagc tttgagcaga
     7261 aatgaaaaga acatcattac aattgatgga gacggagcgt tgcttatgcg catgggaaac
     7321 ctagcaacca acggatacta ctgtccagca aatttattac acattctttt agacaatcaa
     7381 acccatgact ctacaggggg acaacatacg gtctctcata acattaattt tgtagatatt
     7441 gcttcttcct gtgggtatca aaattccatt catgttcata acttatatga gttagagacg
     7501 tatatcaaac agtggaaaag tgataaaaaa ttaacgtttt tatatcttaa aatagcgaaa
     7561 ggatctaaaa agaacctggg gcgtcctgaa atgaagcctt atgaagtcag agaaagactg
     7621 caggtgtttt tagatgatta aaacagctgt tattctagct gcagggatgg ggagcaggat
     7681 tcgtaaaaga gctggaaacc gccctaaagg ttttttgatg attgatgaaa aatcgattat
     7741 tgaacattcc atttccaagt taatagaagc aggagtcaag acgattttta ttggaacagg
     7801 ttatatgaaa gaagaatatg aaaagctaat ggttagatat cctcaaataa aatgtgttta
     7861 taattctaga tacgaaacaa cgggaagtct gtttacgctg tatcaattta aaaattatat
     7921 taaagaagat tttttactat tggaatcgga tgtaatttat gaaaagaatg cgttggaaac
     7981 attagtaaat cattcgagat cagatgtcat actagcgagt aaattgaacg gtgctggcga
     8041 tgaagtttat attgaagctg atgaagataa taaccttaag aatatgtcaa aacaaaaaga
     8101 agagttgaat agtatctatg cagaactagt tggtttaacc aaactctctt atgctacatt
     8161 tcagagactt tgtgatgaag caaatacgct gtttcagttt aatcaaacga tcgattatga
     8221 atatggcctc gttaaaattt ccgaaaaagc aaatgtgtat gttcataaac tggataattt
     8281 ggcatggtgt gaagtggatg atgaagatca ctgggcaaga gcaactacta ttgtttatcc
     8341 gattattaaa gctcgagaag gtattccaca tacagttaaa agaaatatac tcctaaaccc
     8401 cggaccggca actacaacag ataccgtcaa atatgctcaa atagtggagg acatttgccc
     8461 gagagaaaaa caatttggag agacaatgga atttatctct gctgaactaa caaaatttgt
     8521 tgggaatcca gagaagtata ctacggtatt attcggcgga tctggaacgg cggcagtgga
     8581 atcgatttta agttcagtaa ttgataacgg tacggttgtt attatcaata atggtccata
     8641 cggtgaaaga atgtgtaaaa tcgctgaaat ttatggactg aattatttag aatataaaag
     8701 ctccgccgag caggctattg atatgaacga cttaaaaatt tttataaaaa agatttcaac
     8761 gaacgtttcc tatctggcgc ttgttcactg tgaaacttcc acggggcttc ttaatgatat
     8821 tgaacaaatt ggggaatttt gtgcagtgaa aaatatcgaa atgattgtgg atgcaatgag
     8881 ttcgtatgca gctgttccaa ttgacatgca aaaaatgaat ataagctatc tggccgcaag
     8941 tgcaaataag aaccttcaag gaatggcagg cgtgtcgttc gttatagcaa ataaagaccg
     9001 gttagaaaaa acggagcaaa taaagcctcg taacctttat cttcatttat atgatcagta
     9061 tcgatatttc caaatgaata aacaaatgcg ttttactcct cctgttcaga ctatgtacgc
     9121 cttaaagcag gcaattatag aaactcagtg ggaaggaatt gagaaacgat atgaacgcta
     9181 ctcaaaatca tggactactt taacggatgg aattactcgg cttgggttaa cctatttggt
     9241 tcctgaaaca catcattcta aaattattac ctctatcatc gagccttcca atagaaagta
     9301 taattttgat attatgcatg atttttttta taaacaagga ttcaccattt atcctggtaa
     9361 aattgataaa ttagaaacgt ttcgaatagc aaatattggt gatatcacat atagagatat
     9421 agaaagattt cttcaccttc ttgaacaata tcttaaagct ttaaaaggag agtgagaaaa
     9481 agttgaatca aaaattagct gtagcagtta tggtggtaaa tcaagatcat cacgtgcttc
     9541 tagtcaagaa ccatagacgg ggatgggaat ttccaggcgg ttttgttgaa gcaggtgaat
     9601 caataaaaga tgcggggata cgagaagtta aagaagaatc tggaattgtt attgaagtga
     9661 ccaactttct aggagtagaa caagatgtta aacgatctac tactgttatt ttattaaagg
     9721 gaaaagcgat tagcggaaaa atttctgtct ccaatgaaac gcaggatgtg ggatatttta
     9781 cgttagaaga agcaaaaaat ataattaaat tagataactt taaggatcga atggctagat
     9841 gtctaaaaga gactgaaata ccatttttct atgttttaaa agacacgggc tccttttacg
     9901 tataggcgga agatttctgt gttgtgctag aaatgaagcc tgaacgcgat taaataaaaa
     9961 acgctcatcg tcatgcgtga cgatgagcgt ttttttatca gtaaaagtat ggttgtttat
    10021 tcagccgtgg caatttcttc ttctacatca tggtcttcta aattagcact taaaaattca
    10081 cttgtttccg ataaattgag atttaatttc gtttttttgc agatttgaat tgtaacatcc
    10141 tgaacggaga catctaaaac atgcccccct tcttttttat attcatcaat aaagtgcagg
    10201 tggtagccgg gaacaccgat gcctgaagaa tattctggcg tgtaaaaacc tgctagtgtt
    10261 ccatttacat ctgaagatgt aaatgtaggc tgatcagcag ccgcatctac catagaagga
    10321 tagggctttt cttgctttgc aaccgtgcga gtttttactt ctttaaactt gccgtcaatg
    10381 cgaactgcat agaataaatt ggggctagga ataagttcgt tcattaaggt ttctagttgt
    10441 tcacgattca tttccttttc tacacgataa gagatatctt cataaaaggt tgtcataatg
    10501 gcaaaaggtg tttttgcatc ttcactaata ggtgcggctt taccatcagc aatatgataa
    10561 aattctccgt caaaggcaat catttcacca tccaattgat taaacgtacc aatgccgaaa
    10621 tcccctttct tagctaaatc cttgtatgtg acaacaccgt caaatactcc gtctaataaa
    10681 gcgatcatag tggaagtttg gtgaatcgtt aatttttctt tattgctcat tctttactca
    10741 gtcctttcag ttaatttgat ctggcagtag ctgtgtattt aatttttcat tatctttata
    10801 atctacagga acatcgatga taacggggcc ctgttcacga aaagcgcttg ctagagtgtc
    10861 cgaaaattct tctggtgagt taactctaaa tcctttggca ccaaaacttt cagcaagcat
    10921 aacaaagtct acactgccaa actctactgc agattttcgg ccatatttaa tctgctgttg
    10981 aaagccaacc atatcgtagg tttgatcgtt ccagataatg tgaacaattg gtaagtttaa
    11041 ccgaacggct gtctcgagct ccatagctga aaataaaaac ccgccgtctc cagacatgga
    11101 cagaactttt ttatgggggc gaacgagtgc agctgaaata gcccaaggca aagcaacacc
    11161 tagtgtctgc atgccgttgc tgaatagtaa atgctgaggt tcataggaac gaaaataacg
    11221 agccatccat atgtaatgcg aaccaacgtc acatgtgatg gtagtatcat cttcaacaaa
    11281 gttctgcaga gcttcaatta attgtagggg atgaaggtga gtgtcattag aaaactcgta
    11341 ctgatcgtca ggttgttcaa gtttgctttt tagcgttttt aaaaaagaag cactttcttt
    11401 tgaacgtatt aaatgtggga gtttatccgc taatatgtca attgtgcttg gaatatcccc
    11461 tactaactca atttccggct gataatggtg gtcagcttgt gcttgtaaat catctaaatg
    11521 aataatgctt ctttgacttt ctgagttcca aagagaagga gggtattcaa ctgggtcata
    11581 tccgattgtt aagacgacgt cagcatgctt caataacacg tctccaggct gattgcgaaa
    11641 aagacctact cgcccaaaaa agtgagtttc aagctctcgt gaaacagctc ccgcaccttg
    11701 gaacgtttcc accacaggaa ggtcatgtgc tgctataagc tgacggattg attccacttc
    11761 tttagattta cttgctctca tacctaaaag taaaacaggt agttttgctg tgcgaatagt
    11821 gtgtatagct tgggcaacag cgtctaaatt agcagcgcca atcgtaggag cttttaaaac
    11881 ggatagagtg gaagctgatg tttcgttatc cattacatcc tgcggcaagc tgacaaaaga
    11941 tgctcctgaa ggagaagaaa cagcttcgcg aaaggcgtct gttatcactt ctggaacgtt
    12001 tgcagcgtct tctacctcaa cgctgaattt ggcgatagat ttaaatagtc cttcattatc
    12061 cattgattga tgcgtctttt ttaatcgatc tgctcttgga acggctcctg ctagggctac
    12121 cactggatca ccttcagaag tagcagtgac aaggccagtg gctaagttag cggcacctgg
    12181 acctgatgtg acaaggcaaa cgcccggcgt acctgtcagt cttcccatag cagctgccat
    12241 aaacgcagca ttttgttcat ggcggcaaac aattacttct ggtccttttt ctttcaaaac
    12301 atcaaatacg ccatcaattt tggctcctgg aatgccaaaa atataaggca cattctgact
    12361 tgccaaactt tctactagta agtcggcgcc tttttttgtt tcggtagtat cagcattact
    12421 catcatgcac tctctccttt atatttctta aataggtttt aaggcctact gattggatac
    12481 ccaatttgta tgacaactaa cctaaggaaa ggagaaggtt ttaattttct aaataaaaaa
    12541 cttccctatt aattagggaa gcagagatat acctattgcg aataaaacat atctgtttta
    12601 tttgtagttt atggtacttg agggatagca agtgaaagtc ctacgcggct cttatttggt
    12661 acggttaaaa agcttttggt aactaacaat tttttgatct agcttttgac ttaattttaa
    12721 aatctcaccg tgagagtagc tgcttgtttt tttagctaat tggtaaatat ctgattgtaa
    12781 ggctcgaatt tctttttcta actgaggttg cttcattgaa atcctctcca agtcagaata
    12841 cttctatctt ctatgttgta tcttttatct aatagtatga aattccttca tatttgtaaa
    12901 atgttttttg ttttgaaaaa tatgacatag gtgaagagct gctgacaagc tttgtcgaac
    12961 acttttttta cctgttgtca acaagtatga tatactgagg ctaggaaaaa tgacaatctg
    13021 gagcgcacgt tcagttattc ttaacaagcg aatgaattct tagctttgac aagtttagct
    13081 tgtaaggtga agaaattcat cacataatct tattagatct gaaccttgtt agcccggatg
    13141 aggatttgtc gaaaacgatt cactagaaaa aaccttcgag accgccaact cggaggtttt
    13201 tttatttttg tgtatgggaa gtgaaaaaaa gacatagact aataaagaaa aaggtctcgc
    13261 acccataatc ggtcgtagtt gattacacct tgtccatttc tgcaaggaag ctgtcttttt
    13321 actataggga agtgccgcca aacaccattc cctaaagaca atcagtacac gagccttatg
    13381 gatgaagaac cttttagacg atacttatcg gtgattactt attttactac tggaaaaata
    13441 gcaaaataag agcgactatg gctcctaaaa gctctacgta agccgttagg agctgaacat
    13501 tctcagcatc taggaaaaat gacttgagga ttcgtctcag actatccacc acctttttct
    13561 tccttgcgtg aattcccaag ggaaagaaag gggacaacag tgcttcttta ttttaacaaa
    13621 agtaggaaat gataagtaga tttatttgaa gtgtaaatta aaaaagcatg tagaatacat
    13681 gcttttttaa tttggataaa tttcttttat gcgtccgact tgtccatctg ttaaacggac
    13741 tttgatgccg tgtgggtgaa agcttgaatt ggtcagaatg tctttgaccg ttccgtgtgt
    13801 aagttttcca gaacgctgat ctgcttttaa aacaatgtcg acttcaattc caggagctac
    13861 gtcttttcta ttttgtccgt tcattataca cctgttctct ttcgctgatt attcgttttt
    13921 tttgtttgtt gactcgtcag ctttttggtt gcttgattgg tgctgctatt ttgagcttta
    13981 cttgaagatt gatttttctt attagcaagc tgctgtttga tagcatcttg aagactaatt
    14041 ttctttttaa gttgttctgt catatgggtt cctcctgaat atatacatca tctgaaaaaa
    14101 caaagaaagt aagggaatga aaaagctgac tagatagtca gcttttttta attagaaaca
    14161 gtgacttctt ctacaggctg cataagtaca gcttctttat caggatacgt tttttcaata
    14221 tgatgttctc cccaagagca taatgaatca agaatgcttt ttaaagacca tccgtaatct
    14281 gttaaagagt attctactcg aggcggcatt tcattgtaca tttttctaac aatgatgccg
    14341 tctgcttcaa gttcgcgaag ctgctgcgtt aatacttttt gcgtaacgcg cggcataatt
    14401 ctttttaatt ctccagggcg ctttgtccct ttaattaagt ggcaaagaat aacgactttc
    14461 cacttgcctc caatgacttc tagtgccgct tccactggaa tattatattt tttcacgtta
    14521 acaactctcc ttgttctaga gataacggtc aaatgcatgt tttcgcattt tttctgatgt
    14581 ttatcatatc actatagagc aaaattttct aggtgtaagc atttttttag aatacccaat
    14641 agttcatgac tttagtattt acgcttattg taacctccag gtgcctatat cacttgaaag
    14701 tgcgtacttt ttctttggtt ttacatcgct cataataaca attgttgatg gcgagttagc
    14761 gaatatatga aaggtaccta agctaatcag ccgtaagaga agctattatg aagaagttat
    14821 cataacgaac aggaggcgtt aagaagtgaa cacactttca caaacacaac aaagtaaaaa
    14881 aagcggtgct ttgccgctat tagcgctagc tattagcgcc tttggaatcg ggactacaga
    14941 gtttgttcca gtaggtttat tatcatcgct tgcagatgat ttaaatattt ccattacact
    15001 agcaggatta ttaatttcag gatacgcaat gggagttgca tttggagctc ccgtattaac
    15061 agctttaaca agtaaaatga gcagaaaatc actgttgatg ctattgatgg ttatttttat
    15121 cgtaggaaac tcagtagcag ctttatcaac aagctttggg ctgctgctta tagcacgaat
    15181 tattactgca ttttcacacg gaattttctt ttccatcggt gcgactatcg ctgctgattt
    15241 ggtgccggaa gataagcgag caagtgccat tgctttaatg tttacaggtt tgacagttgc
    15301 aactgttaca ggtgttccgc ttggtacatt tatcggacaa gctttcggct ggagagcaac
    15361 attttgggga gtagcgttac ttggggttat tgccattatt gcaagtgctc ttttagtgcc
    15421 taaaaactta aaggaagcgc cgccagcaaa gtttagtgat caattgaaaa ttttgagaaa
    15481 cgggccgctt ttactatcgt ttgctattac cgccttagga tatggcggaa cgtttgtagc
    15541 ttatacgtat ttagcaccta ttcttgaaaa actaacagga tacgcaccaa gtgcggttag
    15601 tatcttgcta ctggtatacg gtgtagcagt agccatcgga aatacgatcg gaggaaaagc
    15661 tgcgaataaa aacccgttaa aagcattatt ttggatgttt ttaattcaag cagttatctt
    15721 agttatttta tcgtttactg catcgtttaa agtagcggga acaattacga tttttgccat
    15781 gggcttattg gcatttatga acgtacctgg actgcaagta ctggttgtga acttagctga
    15841 aaagtatgtc ccttcagcag tgaacgtagc ctctgcgctt aatattgctg catttaacgt
    15901 tggaattgct attggttctt ttgtaggcgg cctgattgtt gattcgattg gtcttgttca
    15961 tactccttgg attgggggag ttatggtttt aggagcggtc ttattaacag catggagccg
    16021 ctcaatagaa gcaaaagcaa aataaaacaa gagatcatcg ccttttgcga tgatctctta
    16081 gctataaaag gagaggaagc aaatgactac atttcaacat cataaggcat atcgtcgtat
    16141 gtacaagaag aatgagttaa cgttaggttt gcatatgcca cttgaaaatt atcaatatgc
    16201 cacgcctacg atggagaaac aagtagagct cacgcagctt gcagaacaat atgaatttac
    16261 aagcttatgg ttccgcgatg tccttcttca agatccatca tttggcgatc cagccacggg
    16321 tcaaatttat gatatgctta tttatttgac ttatctggcc agtcaaacaa aagaaattgc
    16381 tcttagtaca gctgctattg tgctgccttt gcgccatccg cttcgcgtag ctaaagaaat
    16441 ggcaactatt gatcagcttt ttcctgagcg tttgattatg ggcgtatctt ctggagaccg
    16501 tcaagcagat tttcgagggt taggtattcc tcatgaagaa agaggaaagc gatttgcaga
    16561 agcttttcat tatttagaag aagcgttaaa taaagaattt ccatctatcc agtctgcttt
    16621 gggatccatt caaggagcaa atgtcgttcc taagccaacg aaacgtattc caacgatgat
    16681 cacgggatac agtcagcaaa atatggagtg gtttgcacag tatggagacg gctggatgta
    16741 ttatcctcgt cacccccatt ttcaagcaga agctattaca caatggagga aactggttga
    16801 catctatcat ccaggcgttt ttaaaccttt tacacaacct atgcatctag atttggcaga
    16861 agatccaaat gaaatgccaa cgccgattcg tttaggattt cgagcagggc gcaatgcatt
    16921 agttgagctt ctttctattt ataaagaaat aggagttaat cacctgtttt ttgctctttt
    16981 tgatagtaag cgtccggctg aggaagtcat acatgagctt ggtgaagaag tgcttccgca
    17041 ttttccgtct catatgtagc aaaaagaggc tgggacaaaa gtagtttagt taaagggaaa
    17101 tccgaacgat gaatcgagat tcttaatgga gaatctaact cgttcggatt tttttattgg
    17161 taggatgaac gtagcattta tgtatgtagt tgcttctagc ggttgattgg aagacaagac
    17221 gaagactcct ggggggctcg cgggccgccc gcggaaagcg aagtcttgca cggaagtcaa
    17281 ccgtgatgga acaagcgacc catatgagct cctttatcca attagttcgt ctttagattc
    17341 cattgatttc gttatgtctc aatctctttt tatatagatt tcaagttata ggatatataa
    17401 gaaatatgta ttagagttat tgatcc
//
