LOCUS       NZ_CP059405            13079 bp    DNA     circular CON 14-APR-2024
DEFINITION  Bacillus velezensis strain MV2 chromosome, complete genome.
ACCESSION   NZ_CP059405
VERSION     NZ_CP059405.1
KEYWORDS    RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 4191702)
  AUTHORS   Chen,X.H., Koumoutsi,A., Scholz,R., Eisenreich,A., Schneider,K.,
            Heinemeyer,I., Morgenstern,B., Voss,B., Hess,W.R., Reva,O.,
            Junge,H., Voigt,B., Jungblut,P.R., Vater,J., Sussmuth,R.,
            Liesegang,H., Strittmatter,A., Gottschalk,G. and Borriss,R.
  TITLE     Comparative analysis of the complete genome sequence of the plant
            growth-promoting bacterium Bacillus amyloliquefaciens FZB42
  JOURNAL   Nat Biotechnol 25 (9), 1007-1014 (2007)
   PUBMED   17704766
REFERENCE   2  (bases 1 to 4191702)
  AUTHORS   Lee,H. and Jo,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2020) Microbiology, Pusan National University, 2,
            Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JUL-2020
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 589.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013875015.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:37:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,290
            CDSs (total)                      :: 4,172
            Genes (coding)                    :: 4,053
            CDSs (with protein)               :: 4,053
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 56 of 119
            Pseudo Genes (incomplete)         :: 72 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 24 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:30:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1711297
            Orig. end                         :: 1724376
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059405.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..13079
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..13079
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13079
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:8079]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8079
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(549..1598)
                     /locus_tag="H1Q60_RS08800"
                     /old_locus_tag="H1Q60_08800"
     CDS             complement(549..1598)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) delta12_cd03507"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014419303.1"
                     /locus_tag="H1Q60_RS08800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08800"
                     /product="fatty acid desaturase"
                     /protein_id="WP_061862603.1"
                     /sec_met_domain="delta12_cd03507 (E-value: 6.7e-102,
                     bitscore: 339.5, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINHAATQKQASLKKQVAAFSGADTKHSVIQLFNTFVPFFALWFL
                     AYFSLDVSYLLTLAFTVIAAGFLTRIFIIFHDCCHQSFFKQKKLNRLFGFISGVLTLFP
                     FLQWQRSHSIHHATSSNLDKRGTGDIWMMTVKEYNEASAWTKIRYRLYRNPFIMFILGP
                     IYVFLIKNRFNVKGARRKERWNTYFTNAAIVLLAAATCLLVGWENFLLVQGPIFLISGS
                     IGVWLFYVQHTFEDSYFEADEHWDYVQAAVEGSSFYKLPKLLQWLTGNIGYHHVHHLSP
                     KVPNYKLEAAHEGHEPLKNVPTITLKTSLESMKFRLWDEDEKQFVTFREARKTPLPQPV
                     PKEPLKKNA"
     misc_feature    1753..1767
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 17.03"
                     /tool="antismash"
     gene            complement(1845..3002)
                     /locus_tag="H1Q60_RS08805"
                     /old_locus_tag="H1Q60_08805"
     CDS             complement(1845..3002)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118855.1"
                     /locus_tag="H1Q60_RS08805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08805"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_134986776.1"
                     /transl_table=11
                     /translation="MPDSVTTDIKQALLVMLGERDIRRVTMKDIAERAHVSRGTLYLYY
                     EDKYAILEDIAEEMKDGLSEALYDSLKHKDVLHLNGGWQKVHPTLSFVDRHRAFFKTMM
                     DRQKMPYFHFHAFFKDVFRHDVLLSPVNANFSPTEQDMYIHYRSLYTYAIVLYWLNEEP
                     GASPEAISGQVWDLISQKRFYWLFGRRVPEQEEKKPADRRVARTRQALQEAMIGLAAEK
                     KDYAAITISDIARESNLRRATFYDHYANKEALLKTIIHQSCRELIGLLTVKGDPADCSM
                     EEAEAALVRLFSALSEGMPLVHFLREGSGVPDVIPEMFRALQSFYLHQPLHIQAGKKLY
                     AYYVASMLVGLIQYRLHEGKDHAPDKLAREFLQFLDVKKYRVILL"
     gene            3144..>3327
                     /locus_tag="H1Q60_RS08810"
                     /old_locus_tag="H1Q60_08810"
                     /pseudo=""
     CDS             3144..>3327
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118856.1"
                     /locus_tag="H1Q60_RS08810"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="H1Q60_08810"
                     /product="aldehyde dehydrogenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDQHIKATSNASLSFRKKSKNTACFNSRAAQRKASALFRCRHRS"
     misc_feature    3169..3182
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.31"
                     /tool="antismash"
     gene            complement(3349..>3621)
                     /locus_tag="H1Q60_RS21365"
                     /pseudo=""
     CDS             complement(3349..>3621)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611967.1"
                     /locus_tag="H1Q60_RS21365"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="KYVPCPLLENCTKSKNRKKVILLSMYR"
     misc_feature    3860..3876
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.37"
                     /tool="antismash"
     gene            3972..4196
                     /locus_tag="H1Q60_RS08815"
                     /old_locus_tag="H1Q60_08815"
     CDS             3972..4196
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664240.1"
                     /locus_tag="H1Q60_RS08815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08815"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025650419.1"
                     /transl_table=11
                     /translation="MNNKVKVERLTLGLTQATLAEKLGITRQTVSLIEKGQYNPSLNLC
                     IKICNVFNKTLDDIFWPSDIKREDRENDR"
     gene            complement(4741..4959)
                     /locus_tag="H1Q60_RS08820"
                     /old_locus_tag="H1Q60_08820"
     CDS             complement(4741..4959)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H1Q60_RS08820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H1Q60_08820"
                     /product="hypothetical protein"
                     /protein_id="WP_025650417.1"
                     /transl_table=11
                     /translation="MLHNFEKSNNMIELSEQEMLDTDGGGWRDLAGAAAGAAVMGGTAM
                     ALGAAGGPVGFAAGIGLAILTRPGHAK"
     gene            complement(5001..5222)
                     /locus_tag="H1Q60_RS08825"
                     /old_locus_tag="H1Q60_08825"
     CDS             complement(5001..5222)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /locus_tag="H1Q60_RS08825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08825"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_025650416.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.9e-08,
                     bitscore: 30.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKQNNRFESLTVEEAEMIDGGGYGSYLLKGVGTGIAVGAALGGP
                     GGAVLGANAGAIWGSAAYGVDALISGHK"
     misc_feature    5287..5302
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.12"
                     /tool="antismash"
     misc_feature    5456..5474
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.12"
                     /tool="antismash"
     gene            5517..5777
                     /locus_tag="H1Q60_RS08830"
                     /old_locus_tag="H1Q60_08830"
     CDS             5517..5777
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H1Q60_RS08830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H1Q60_08830"
                     /product="hypothetical protein"
                     /protein_id="WP_025650415.1"
                     /transl_table=11
                     /translation="MDFLYMLVETILWIIITTIPSKRNLKFRRNLKSLKKQAWFCELKK
                     NYGPLFLMNSTIQDKIIEYNENNDLETYKEEIENLAKSIHV"
     gene            5896..8079
                     /locus_tag="H1Q60_RS08835"
                     /old_locus_tag="H1Q60_08835"
     CDS             5896..8079
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014481436.1"
                     /locus_tag="H1Q60_RS08835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08835"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_025650414.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.2e-43, bitscore:
                     144.7, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 1.3e-234, bitscore:
                     778.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFFKKYHCVKQMDEKDCGAACLSTIARQYGSKLSITYIRDIAGTD
                     TQGTSVLGMVKAAEQLGFSAKAVRAEHAAFDQSITLPAIAHIVIDQQLLHYVVIHKISK
                     EKIIIADPGKGLIKYKKDDFLKIWTGILILLTPTEAYEKQKEIRNISSDIFRLILQQKS
                     LIIHTFLASFIITIFGILGTFYFQTIIDQILPNGLSNTLHILSIGLLIMYLFKVFLIGF
                     RQYMMILLGQKLSITIMFGYFKHVLSLPMKFFSNRKVGEIISRFTDANKVIDAVVSASL
                     SILLDTIMLIMVGIFLYLQNATLFLITLAFVPLYILTVWIFIKPYANINNTEMENNAQL
                     TSKIVETLNGIETVKAYNAEYMMTFETEKRFVKYLRSAFKHGVIDNLQSSIKMFLDLAS
                     GALILWIGSTQVMKGNLTIGQLITYNALLTYFLNPLQNIIGLQSKFQSASVASKRLGEI
                     IEQDPEIKDNEKRITSISSITGPIVFNKINFRYGTRRLILKNIALQIQKGEKVAFVGES
                     GSGKSTLTKLLMKFYAAESGEILINGYHINDIHTNALRESISYIPQESHFIQGSILENL
                     LLGNSGSFTFEEIIEVCKQTKVHDFIDHLPMRYDTLIEENGSNLSGGQKQRLAIARALL
                     RKPNILIMDESTSNLDTTTEQGISKMIYDQTYNITTIIIAHRLSTIMNCDKIFVMENGE
                     IKESGSHHDLLMKKGKYYELWENQIPSKEMVTI"
     misc_feature    complement(5898..5911)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.67"
                     /tool="antismash"
     gene            8076..9545
                     /locus_tag="H1Q60_RS08840"
                     /old_locus_tag="H1Q60_08840"
     CDS             8076..9545
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332544.1"
                     /locus_tag="H1Q60_RS08840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08840"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_025650412.1"
                     /transl_table=11
                     /translation="MKRNFFTLQELKDSRELYESKPKPIGLIFVYLLIGILISAIAFSF
                     YGKKEVILKTTADIQYSKPNSNVTNEIAGKVKTVSFKQGDKVKKGDTLFTLDDTEIQTQ
                     RKASNADINDLKEQIALNKTLKKSIENDKNLFSEDDHWSFQDQYLSYKKQINLLETSEE
                     TVKTQKEKIDKHTKNVKNNLNKTKSKVTLKLQRISDLQNSIRRDKSFAKQDDKYSSYYN
                     SYLTSKQNAEQQLKDVEKMKDELDKSSYSEQKENLNGQIESLKQDMLVKLSEEKDELKQ
                     SLDEINSSIESDNTDIDSNDSEQTNYRLSIEQLKTNLLNQTAQKQTDLQTTLKAKEDDL
                     KMIDLQLEKYKIKAANDGYVNVLTNISPGDNLQAGTSILTIIPDKEKLKANIYIPSYEI
                     TNINEKDEVKFHLENTSQTIKGVITNISRDSISNQKGQKFYIAESTIANNEIVKDNGEK
                     IHLKNGMQTNVSIIVDTKRYIDYFLDVLHLH"
     misc_feature    9567..9584
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            10021..11358
                     /locus_tag="H1Q60_RS08845"
                     /old_locus_tag="H1Q60_08845"
     CDS             10021..11358
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391865.2"
                     /locus_tag="H1Q60_RS08845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08845"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_007614893.1"
                     /sec_met_domain="Aldedh (E-value: 4.6e-95, bitscore: 316.8,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQHIKADIKRVFELQKKNQKTLRASTAGQRREKLQRFLDAVTAH
                     EEEIIEAIRRDVRKPYHEVKRAETQGTIKAIKDNMNNLDTWMAPKRVGSSVSPDADGVL
                     MYEPKGVTLILGPWNYPFMLTMAPLAAAIAAGNSAIVKLSDFTMETSHIAATVIREAFH
                     EEEVAIFEGEVDVATELLDQPFDHIFFTGSTNVGKIVMTAAAKHLSSVTLELGGKSPTI
                     IDSGYDLMDAAKKIAVGKFVNAGQTCIAPDYLLMKKEVQSTFTDLLQTIVNAGFMEDDH
                     TVDRNKFTQIVNDRNFNRVKALYDDAIEKGAKTAFGGVFDAHDRTISPTVLTNVTPDMK
                     IMQEEIFAPILPIMNYEDISEAVDYITERDKPLALYVFSHNQELIDYVLQHTTSGNASV
                     NDVVVHFTDVNLPFGGVNTSGIGSYHGVYGFKEFSHEKGVFIQASK"
     misc_feature    10047..10060
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.31"
                     /tool="antismash"
     gene            complement(11398..12614)
                     /locus_tag="H1Q60_RS08850"
                     /old_locus_tag="H1Q60_08850"
                     /pseudo=""
     CDS             complement(11398..12614)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012118857.1"
                     /locus_tag="H1Q60_RS08850"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="H1Q60_08850"
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKTLAAGAAVAAALSVGAVSDLPGAKWLHPAAAQAAETVFKQNHA
                     ASGFLAGRYDAQAMSPTMFNWSRESRFTSTADGALKWEKNVPANPQNGAGAAVDGDGTV
                     FIQSKDGKLTAYHPDVL"
ORIGIN
        1 tggcactgat cagtgtaatg acgataaacg gaatttgtgt caaaaaccac ccttttttca
       61 gaacaaggct gaagttgacg gcaaccgccg cactgatgat atgaatgtaa tataagatgt
      121 aaaaaggaac tttgtctcgg atatggccga tgaagtaggc gataaagaag gcgaaataaa
      181 tatagctgaa cagcataact gagccggtcg aaatgccgat cagaagaagt gcccataagt
      241 aaagcggcca gccttttgca acaaatgcaa agcggtatgc gccaaagaaa acaagtgtga
      301 aaatgatgcc ggccacgatg acaaaggtag aagagctttt aaaaataaaa taaaaaggca
      361 agatgaaaaa aatcgtccat atataaggag aaatcccatt cagtttttga aatttaaaat
      421 acttcttcat ctttgttcca ccttctgctg cttgtgtcta ccatcacttt atcaaaacac
      481 cgccttcatt gcatcctcac gtatgaagag aaggcccttt ctgataagag aaagagccgc
      541 gcgccatatc aggcattttt ctttaaaggt tctttcggta ccggttgggg cagcggtgtt
      601 ttccgcgcct cccgaaaggt gacaaattgc ttttcgtcct catcccacag acggaatttc
      661 atggattcaa gacttgtttt aagcgtaatg gtcggtacgt tttttaaagg ctcgtgcccc
      721 tcatgagcgg cttccagctt gtagttcggc actttcgggc ttaagtggtg gacatgatgg
      781 tagccgatat tgcccgtcag ccattgcaaa agcttcggaa gcttgtaaaa ggagcttcct
      841 tctaccgcgg cctgcacata atcccaatgc tcatcggctt caaaataaga atcctcaaac
      901 gtgtgctgaa cataaaagag ccagacgccg attgaccccg aaataaggaa aatcggaccc
      961 tgaacaagca aaaagttttc ccagccgaca agcaggcacg tcgcagcagc caataaaaca
     1021 atcgccgcgt tcgtgaaata ggtgttccag cgttccttgc ggcgggcgcc ttttacattg
     1081 aaacggtttt taatcagaaa tacgtaaatc gggccgagaa taaacataat aaacggattg
     1141 cgataaagtc tgtagcgtat ttttgtccaa gctgacgctt cgttgtattc cttgacggtc
     1201 atcatccaaa tatcgccggt gccccgttta tctaagttgc tgctcgtcgc atgatgaatg
     1261 gagtggctgc gctgccattg aaggaacgga aacagtgtca gcacacctga aataaacccg
     1321 aacagccggt tcagcttctt ttgtttgaaa aaggattgat ggcagcagtc gtggaaaata
     1381 ataaagattc ttgtcaaaaa tcccgctgct ataacagtaa aggcgagggt cagaagatag
     1441 gagacatcga ggctgaagta agcaagaaac caaagggcaa aaaacggcac aaacgtgttg
     1501 aaaagctgaa tgacactatg ctttgtatcg gcgccggaaa atgcggcaac ctgctttttt
     1561 aaagacgctt gtttttgagt tgcggcgtga ttgatcatga atgtcatgtc ccttcttttt
     1621 cattatgatc ccaagtataa cggggcgcca gacgcttttg tagtagcagc catcatgccc
     1681 cgaatatgac atttgtcatg tttggcggca aaatgctgat caggcgtcat atgaaggttt
     1741 cttttttata agggataacg ccggacactt catagcggca cttctcaaag aaaacagcaa
     1801 aaaagaaccc ggcctgtttt cggctgagtt ctttttcatc atcttcacag caaaatcacc
     1861 cgatacttct tcacatccaa aaactgcaga aactcccggg ccagcttgtc cggcgcgtgg
     1921 tcttttcctt catgaaggcg atattgaatc agaccgacga gcatggatgc gacatagtat
     1981 gcatagagct ttttgcccgc ctgaatgtgc agaggctgat gaagataaaa cgattgcagc
     2041 gctctgaaca tttccgggat gacgtcaggc actccggaac cctctctcaa aaaatggacg
     2101 agcggcatgc cttccgacag tgcggaaaag agccggacga gcgcagcttc cgcttcttcc
     2161 atggagcagt cagcaggatc tccctttacc gtcaataatc cgatcagctc ccggcatgat
     2221 tgatgaatga tcgttttcag caacgcttct ttgttggcat aatggtcata aaaagtggca
     2281 cggcgcaggt tgctttcacg ggcaatatcg gaaatcgtaa tcgccgcata atcctttttt
     2341 tccgctgcta agccgatcat agcctcttgt aaggcctgcc tcgtccgtgc cacccgccgg
     2401 tcggcgggct ttttttcttc ttgttccggc acccggcggc cgaacagcca gtaaaaccgt
     2461 ttttgagaaa tcaaatccca cacctgcccg ctgatcgctt ccggtgatgc cccgggctcc
     2521 tcattcagcc agtacaacac aatggcatac gtgtataaag agcgataatg aatgtacata
     2581 tcctgttcgg tcggtgagaa atttgcattc accggtgaca aaagcacatc atgccggaat
     2641 acatctttaa aaaaagcatg aaagtgaaaa tacggcattt tttgacgatc catcatcgtt
     2701 ttgaaaaacg cccgatgccg gtcaacaaaa gacagggtcg gatgcacttt ttgccagccg
     2761 ccatttaaat gaagcacatc tttatgcttc agcgaatcat acagcgcttc actcagcccg
     2821 tccttcattt cttctgctat atcttctaaa attgcatatt tatcctcgta ataaaggtac
     2881 agtgtccccc ggctcacgtg cgcccgttcg gcgatatcct tcatggtcac acggcggata
     2941 tcccgctccc caagcataac caagagcgct tgtttgatgt cagttgtcac agaatccggc
     3001 ataagagaca ctcctttcat tagacaaatc accgcaattt gtacggtaac gtatagatgc
     3061 gtttacgctg tctttgccgc aaaatcacat cacactataa taggccctga agaaacaaaa
     3121 aagcaaccag aggaggaaga ccgatggatc agcatataaa agcgacatca aacgcgtctt
     3181 tgagcttcag aaaaaaatca aaaaacactg cgtgcttcaa cagccgagca gcgcagagaa
     3241 aagcttcagc gctttttaga tgccgtcacc gctcatgaag aagaaatcat cgaggcgatc
     3301 agacgggacg tccgcaagcc ttatcatgag gatggcgccg aaacttcttc atgtccggca
     3361 ggcggatgta aggagaactt gctcgctcac attccgcttt tacccaacct gcaatagcga
     3421 agcccatgca gctgtttgga atatctgcaa agcttcgctc tattttttat tttctttttt
     3481 tataaagttt ttttcttgaa acagacaagc gattttgtct gaccttttcc ctatggttta
     3541 cctatacatg ctaagtaata taactttctt ccggttcttt gattttgtgc agttctcaag
     3601 cagtggacat ggaacatatt tcaggatttg attgatatga ccggcagcct tgtagtcagt
     3661 tgttgagtat gtagtgtttg ctgatttcga gaaatgtatc tatcttgttc attgtcataa
     3721 tgaatttcca ctttggaaac aatcctctta aagagaagat aacgtctata tgcaaagaca
     3781 ccatccttaa atatccattc tcatgtacaa tacaagaaat tcttctcaaa acacagtcat
     3841 ttattgaaag tcacttaact tgcaaaagtg ttccaatagc ttatttcagt tttagaatcc
     3901 aaatctacgc aatatatatt ttgcaaaaag gaatatatat gttacaatat taattaataa
     3961 aggaggatgc catgaataat aaagttaaag tcgaaagatt aacacttggt ttaactcaag
     4021 caacattggc tgaaaaactt ggaataacaa gacaaactgt gagcttaata gaaaaaggac
     4081 agtataaccc cagtttaaac ttatgtataa aaatttgtaa tgtttttaat aagactttag
     4141 atgatatttt ttggccttct gatattaaga gagaggatag agagaatgat agatgaaaga
     4201 atacaacacg aacaatataa actgagctat tacagtttcg gactgtctat aattttattt
     4261 attcttagtt tttttgtttc tattctaacc aaaaacttat tttacactca actaagtata
     4321 attatatcgt ttttagttgt cagcactttt tattcaattt tcgcaataaa aagggggtta
     4381 ttaatacaga gaaaaagaaa aagattaaaa catttcttta tttttatatt gagcggatta
     4441 ttttgggtaa tgataagttt tctttctaaa gaaattacca atgtgagaga attactgatt
     4501 cagttattat tattctcatt tttctacatt gtattatttc taagtgtcca ttctttgatt
     4561 aagtattctc ataaaaaatc taattagtcc attaaattgg ctaagtaaac cttgtgagga
     4621 attaacacaa attatttagt tttcgttctt caatttaaaa ctgcatggaa aggtatgcag
     4681 ttaagattcc ttttaataca gaaaagctgt cggcataaga cagcttttct ataacacaaa
     4741 ttattttgca tgccctggtc ttgttaagat tgccagccct attcccgcag caaaacctac
     4801 tgggccacca gcagctccta aagccattgc tgtacctccc attactgcag cgcctgcagc
     4861 agcgcctgct aaatctctcc agcccccgcc gtcagtatct agcatttctt gttcacttaa
     4921 ttcaatcata ttattacttt tttcaaagtt atgtaacata ataaacctcc tatatataat
     4981 ttttgttcaa tttatgtttt ttatttatga ccagaaatta atgcatctac accatacgct
     5041 gcagaacccc agatagctcc agcattagca cctaaaacag ccccgccagg tcctccaagc
     5101 gctgctccaa cagcaattcc tgttccgact ccttttaata agtaagaacc gtatcccccg
     5161 ccatctatca tttcagcttc ttctactgtt aaagactcaa atctattgtt ttgcttttcc
     5221 atactactac tcctcctaag tccgaacttg gtaaattatt gtcgacttgt attagtataa
     5281 atgtgattta cacttttttc aatactcaaa agcaaaatta catgacattt ttcatataaa
     5341 attcccacaa aacgtctatt tgttacaata accactctct tttttatgat tctcactgaa
     5401 gaagatttaa tcttatactc tgattaatta attgaaactt taactaaaag tacttttatg
     5461 ttagtcttac attatgtttt atttacattg tgaattaagc tcaaggagga catttgatgg
     5521 attttttgta catgctagtt gagactatat tatggattat tattactacc ataccatcaa
     5581 agagaaattt gaaatttaga agaaacttaa aaagtctaaa aaaacaagca tggttttgtg
     5641 aattaaaaaa gaattacgga ccgttgtttt taatgaattc aactattcaa gacaagatta
     5701 ttgaatataa tgaaaataat gatttggaaa cttataagga agagatagaa aatttagcaa
     5761 aaagtataca tgtataaaat ttttttattc aaaaacaaat atatttcctc ccggaactat
     5821 tgatatattc atacttattg tattatgata ataatttttg tataattcaa caattctata
     5881 atatggagat taaaaatgtt ttttaaaaaa tatcactgtg taaaacaaat ggatgaaaaa
     5941 gattgtggag ctgcttgttt atcaaccatt gcaagacaat atggttcaaa gctctctata
     6001 acttatatcc gtgacattgc cggaactgat acacagggaa ctagcgttct tggaatggta
     6061 aaagccgctg aacaattggg gttctctgca aaggcggtgc gagcagagca tgcagctttc
     6121 gatcaatcta ttactcttcc ggcaatagca catattgtta tagatcaaca attattgcat
     6181 tatgttgtta tacataaaat cagtaaagaa aagataatca ttgcagaccc cggcaaaggg
     6241 cttattaaat acaaaaaaga tgatttttta aagatctgga cagggatctt aatactttta
     6301 acacccaccg aagcctatga aaaacaaaaa gagataagga atataagttc agatattttc
     6361 aggttaattc ttcaacaaaa atccttgatt atacatacat tcttagcatc ttttattatt
     6421 acaatcttcg gaattctggg tactttttat tttcaaacga ttattgatca aatcttgccg
     6481 aatgggctaa gtaacacatt acatattctt tcaatcggcc ttttaattat gtatctattc
     6541 aaagttttct taataggatt taggcaatat atgatgatcc tgctgggtca gaaattaagt
     6601 attaccatta tgttcggata cttcaaacat gtactttctt taccaatgaa gtttttcagc
     6661 aaccgaaaag tcggagaaat catttcgaga tttacagatg ctaataaagt aattgatgct
     6721 gttgtcagtg catccctttc tatcctgctc gatacgatta tgcttattat ggtcggtata
     6781 tttctgtacc tgcagaatgc tacgttgttt ctcatcactt tagctttcgt accactttat
     6841 attctgacag tttggatctt tataaagccc tatgcaaata taaataacac cgaaatggaa
     6901 aacaatgccc agctaacatc gaaaattgtt gaaacgctta acggcattga aacagttaag
     6961 gcttacaatg ctgaatatat gatgactttt gaaacagaaa aaaggtttgt gaaatactta
     7021 cggtcagctt ttaaacatgg agttattgat aatctccaga gttccataaa aatgttttta
     7081 gatttagcaa gcggtgcatt gatactgtgg atcgggtcta cccaagtaat gaaaggcaat
     7141 ctcaccatcg gtcagctcat tacgtataat gccttattaa cctacttttt aaatccttta
     7201 caaaacatta tcggccttca atccaagttt caatcagctt ctgtcgcttc taaaagactc
     7261 ggtgaaatta tagagcaaga tccagaaatc aaagacaatg agaaacgaat aacatcaatt
     7321 tcttcaataa ccggccccat tgtttttaat aaaattaatt tccgctacgg aactcgaagg
     7381 ctcatcctaa aaaatattgc actgcaaatt caaaaagggg aaaaagttgc ctttgtcgga
     7441 gaaagcggtt cagggaaatc tactttgact aagcttttaa tgaagtttta tgctgccgag
     7501 tcaggcgaga ttttaatcaa tgggtatcat ataaatgata tccacactaa cgcgttaagg
     7561 gaaagcataa gctatatacc tcaagaatca cattttatac aaggatctat tcttgaaaac
     7621 cttttgctcg ggaattcagg aagttttacc tttgaggaaa ttatagaagt ttgcaaacaa
     7681 acgaaagtac acgattttat tgatcattta ccaatgagat acgatactct tatagaagaa
     7741 aacggatcta acttatctgg cggtcagaag caaagactgg caattgcaag agcattattg
     7801 agaaaaccta acattctcat tatggatgaa tccacaagca atcttgatac gactactgaa
     7861 caaggaatat caaagatgat atatgatcag acttataata taacaacgat tatcatcgct
     7921 catcgtttaa gtacaatcat gaattgtgac aaaatatttg tcatggaaaa tggagaaatc
     7981 aaagagtctg gaagtcatca tgatttgtta atgaaaaaag gcaagtacta tgaactgtgg
     8041 gaaaaccaaa taccatcaaa agagatggtg acaatatgaa acgaaacttt ttcacattac
     8101 aagagttgaa agacagcaga gaactttatg aatcaaagcc aaaaccaatc ggtctaatct
     8161 ttgtatattt actcataggc atattaatat cagccattgc tttcagtttc tatggcaaaa
     8221 aagaagtcat tctaaaaaca acggctgata ttcagtacag taagcctaac agcaatgtaa
     8281 caaatgaaat agcgggtaaa gtaaaaacag tttcttttaa acaaggggat aaggtgaaaa
     8341 agggagacac tttgtttact ctggacgata ccgaaattca aacacaaaga aaagcctcta
     8401 atgctgatat taatgattta aaagaacaaa tcgcattaaa caaaactctt aaaaagagta
     8461 ttgaaaatga caaaaactta ttcagcgagg atgatcattg gagttttcaa gatcaatacc
     8521 tctcttataa aaaacaaata aacttgttag agacatcaga agaaactgta aaaacccaga
     8581 aagaaaaaat cgacaaacat acaaagaatg tgaaaaataa tctgaacaaa accaaaagca
     8641 aggttacatt aaaacttcaa agaatttctg atctgcaaaa ctcaattaga agagataagt
     8701 cttttgccaa gcaggatgat aaatacagct cttactataa tagttatttg accagcaagc
     8761 aaaatgcaga acagcagctg aaggatgtag aaaagatgaa agatgaactt gataaaagca
     8821 gttattcaga gcagaaagag aatctgaacg gacaaattga aagtttaaaa caagatatgt
     8881 tagtaaaact aagtgaagaa aaagacgaat taaaacaatc ccttgatgag attaatagct
     8941 caattgaaag tgacaatact gacattgatt ccaatgattc agagcaaacg aattataggc
     9001 tttctattga acagcttaaa acaaacttgc tgaatcagac agcccaaaaa caaacagacc
     9061 ttcagaccac tcttaaagca aaagaagatg atctgaaaat gatagattta caattagaaa
     9121 aatataaaat aaaagcggca aatgatggct atgttaacgt attgacaaat atatccccag
     9181 gagacaattt acaagctgga acgtctattt tgactattat ccctgataaa gagaaactta
     9241 aagctaacat ttacattcca agttatgaga tcacaaatat taatgaaaaa gatgaggtta
     9301 agttccatct tgaaaataca agtcaaacca ttaaaggcgt aattactaac ataagccggg
     9361 acagtattag taatcaaaaa ggccaaaagt tttatattgc agaaagcaca atagcaaaca
     9421 atgaaatcgt taaagataac ggcgaaaaaa tacacttaaa aaacggaatg cagactaatg
     9481 tatcaataat cgttgataca aaaagataca ttgattattt cctagatgta ttgcacttgc
     9541 actaaatttg ttaaaagtag ttctcatatg cctcaaagcg gaaagaagat ttgctttgag
     9601 gcattttttt cagaaatcca aatgctacac cttatgatct tctttgaaat ccagcacttg
     9661 gtcatctcgt ttgcaaaata attcttcaca gcctccaact gaaatgctac actttttccg
     9721 cccgcctatt cctaaaacac atcttataaa aagtaaaaat cccggatgtt aactccgggc
     9781 catgtcaaag caataccact tttacttttg gcggatatcc cgctccccaa gcataaccaa
     9841 gagcgcttgt ttgatgtcag ttgtcacaga atccggcata agagacactc ctttcattag
     9901 acaaatcacc gcaatttgta cggtaacgta tagatgcgtt tacgctgtct ttgccgcaaa
     9961 atcacatcac actataatag gccctgaaga aacaaaaaag caaccagagg aggaagaccg
    10021 atggatcagc atataaaagc agacatcaaa cgcgtctttg agcttcagaa aaaaaatcaa
    10081 aaaacgctgc gtgcttcaac agccgggcag cgcagagaaa agcttcagcg ctttttagat
    10141 gccgtcaccg cacatgaaga agaaatcatc gaggcgatca gacgggacgt ccgcaagcct
    10201 tatcatgagg tgaaaagagc ggaaacgcag ggaacgatca aggcgattaa agataatatg
    10261 aacaatctcg acacctggat ggcgccgaaa cgcgtcggct catccgtcag ccccgacgct
    10321 gacggcgtat taatgtacga gccaaaaggc gtcaccttaa ttctcggccc atggaattat
    10381 ccgtttatgc tgacgatggc tccgcttgcg gctgccattg cggccggcaa cagcgcgatt
    10441 gtgaagctgt ccgattttac gatggagacg agccatatcg ccgcgacagt tattcgcgag
    10501 gcatttcacg aagaagaagt cgccattttt gaaggtgagg tcgatgtcgc cacagaactg
    10561 ctcgaccagc cgtttgacca cattttcttc accggaagca cgaatgtcgg taaaatcgtc
    10621 atgacggcag cggcgaagca cttgtcgtcc gtcacgcttg agcttggcgg aaaaagcccg
    10681 acgatcatcg acagcggcta tgatttgatg gacgcggcga aaaaaatcgc cgtcggcaaa
    10741 tttgtcaatg cggggcaaac atgtatcgct cctgactatc tccttatgaa gaaagaagtg
    10801 cagagcacct ttacagatct cctgcaaacc attgtcaatg caggttttat ggaggatgat
    10861 catacggttg accgcaacaa attcacgcaa attgtcaacg accgcaactt taaccgtgtc
    10921 aaagccctgt atgatgatgc cattgaaaaa ggggcgaaaa cggcgttcgg cggcgtattc
    10981 gatgcgcatg accgcacgat ttctccgacc gtgctgacaa acgtcacgcc tgatatgaag
    11041 atcatgcaag aagaaatctt cgcaccgatt ctgccgatta tgaattacga agatatcagt
    11101 gaagccgttg attacatcac tgaacgggac aagccgcttg ccttgtatgt attcagccat
    11161 aatcaggagc tgatcgatta tgttctgcag catacgacgt ccggcaatgc ttctgtcaat
    11221 gatgtcgtcg ttcatttcac tgacgtcaac cttccgttcg gcggtgtcaa tacgagcggg
    11281 atcggttcat atcacggtgt gtacggcttt aaagaatttt ctcatgaaaa aggcgtattt
    11341 attcaagcct ctaaataaaa aaagaatccg cgcagatgac gcggattctt ttttatatta
    11401 ttttgctccg aatgcgagca gtttcgtata agtgccgaca taaagcgtgc cgtcttcagc
    11461 cagaaccggg gaggaaatga cattgctgtc tgtttgatag cgccattttt cattaccgtc
    11521 agcgtcaacc gcatagacgt ttttatcgtc agagccgaag tacacattgc cgtccgcatc
    11581 aatcagcggc gccgttctca caggcccgtc cgtatagaaa gaccagttca tattaccggt
    11641 tttgctgatg gaaaacagct cgccgcttcc ggaaccgata tacacattgc cgttatggtc
    11701 gatcgctaat gaagagctgg agaaaccgtt taacgggaac gtccacttta cagcacccgt
    11761 tgatgtatag gcatataagt tgccgtcctg gtttccggcg taaacggtgc cgtctctatc
    11821 tataaccgga taacctttaa acccgttgga cgtcgttttt gaccatttca cttggcccgt
    11881 tccggaattg atcgcgtaaa agatattatt gtacgttgcc gtatacagag tgccgtttga
    11941 tgctaacacg ggagcggaac cgaccacgcc gtttgtttta aacttccact tctgcgtcca
    12001 tgtcgaaggc gacgtcggtt taatcgcata gatatagtta tccagcgtcg acacatacag
    12061 cgtgccgtca gagccgatag ccgcatcgga actaggcgct ccgctcaacg gaaccgatgt
    12121 taaaatgttt ccggtttctt tgtcgatgaa atacagtttt ttatcgtgag aaggaagata
    12181 aataacaccg tttgttccga gcaccggagt cagcgtatag gtcgtcccga ggttttccgt
    12241 cacccatttc acagtacgtc cggatgatac gccgtcagct tcccgtcttt tgattgaata
    12301 aatacggtgc cgtccccgtc aaccgcggct cccgcgccgt tttgaggatt ggccggaaca
    12361 ttcttttccc attttaacgc gccatcggca gtgcttgtaa accggctttc tcttgaccag
    12421 ttgaacatcg tcggagacat cgcctgcgcg tcataccgtc ccgccaagaa accggatgcc
    12481 gcatgatttt gtttaaatac cgtttccgca gcttgagcag cggccggatg cagccatttt
    12541 gcccccggca gatcagaaac cgcccccaca gataaagccg cagcaaccgc agcgccggcc
    12601 gccaatgttt tcattcgcaa tgaagctgtc ttttttttca atgtcagttc ctcccgtttc
    12661 tattaaaaac tgctataccg ctttcatcat accataatca tcctatgaaa tatttgtatt
    12721 ttcggtagat ttatagaaca aatgtcacat agcctgctga tgcttcgggg atgatcgaaa
    12781 cgcagcaata taccacacca cattgacgat gaaagccgcg cctgaccaaa cgaaaaggcc
    12841 gtaataaaga gacagctcct gccctaagga aattgcggtc aataacgtca ccaacaagac
    12901 agccgcgcaa atgataagcc cgattccgca aaaaacggcc atgatgattt tcatgacacg
    12961 aataccgaga atatacataa tgagcgggac cgcatatata acaaggatct ggccgacggc
    13021 ggcccacatg cccgcggaat taaaacgatt cgcgttcggc cccgtttccg caagcggag
//
