LOCUS       NZ_CP058693            12940 bp    DNA     linear   CON 08-SEP-2024
DEFINITION  Streptomyces sp. Rer75 chromosome.
ACCESSION   NZ_CP058693
VERSION     NZ_CP058693.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Rer75
  ORGANISM  Streptomyces sp. Rer75
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Genome analysis of Streptomyces sp. Rer75
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) College of Pharmacy, Henan University of
            Chinese Medicine, Jinshui East Road No. 156, Zhengzhou, Henan
            450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. Canu V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.63x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013414305.1-RS_2024_09_08
            Annotation Date                   :: 09/08/2024 05:25:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,857
            CDSs (total)                      :: 8,765
            Genes (coding)                    :: 8,460
            CDSs (with protein)               :: 8,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 145 of 305
            Pseudo Genes (incomplete)         :: 204 of 305
            Pseudo Genes (internal stop)      :: 20 of 305
            Pseudo Genes (multiple problems)  :: 62 of 305
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:27:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9387295
            Orig. end                         :: 9400235
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12940
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="38"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12940
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12940
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7940](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7940
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    2013..2022
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(2401..2415)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 21.24"
                     /tool="antismash"
     misc_feature    3118..3127
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.02"
                     /tool="antismash"
     gene            3223..3906
                     /locus_tag="HYQ63_RS39820"
                     /old_locus_tag="HYQ63_39820"
     CDS             3223..3906
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720314.1"
                     /locus_tag="HYQ63_RS39820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39820"
                     /product="PAS domain S-box protein"
                     /protein_id="WP_179938731.1"
                     /transl_table=11
                     /translation="MDEPEVPEEARKILGGEDWVVWRNRSLLLFDRVPVPCAVCRTDGE
                     ILMANPAMAAEWGTAPGQLAGRNVLELFHPNAAAQVHRIVEAVRLGRRSRYPIEVRWQS
                     PDGARRYGELTADTVSDTDSPMWLLVLLRVLGEEPRSRGDGTADPAPDETERAAPIEAR
                     ILALAAGGHTTAQMARQTGLTGDGVNYHLTRLSRRWGVGNRAELVARAYVLGVLSPGVW
                     PPEPS"
     gene            complement(3942..4700)
                     /locus_tag="HYQ63_RS39825"
                     /old_locus_tag="HYQ63_39825"
     CDS             complement(3942..4700)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175420.1"
                     /locus_tag="HYQ63_RS39825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39825"
                     /product="hypothetical protein"
                     /protein_id="WP_257232462.1"
                     /transl_table=11
                     /translation="MRRRRWWLLSPLIPLVRSANRVAKRLFDQEGGGRVRDHLVDRVQL
                     TRSVLGIVASLALIYMYGADGGWSGAATDGAANLFATPLLLLLTGPLVVFGFILYAPPH
                     RRPELRSRLRGPLKTVGWYVLILVAFGVFIYVVGQTTLLHGLHGVMLFAVGLPLMVGLP
                     WALVFLFLSSAYVARSAFNTAYVHAALPPVLTTVLVWLIAGFNLLSDGLPNGPLPAQLA
                     AAFGGPLSVTALSLWELRRLRTHHGVTMRG"
     gene            5001..6194
                     /locus_tag="HYQ63_RS39830"
                     /old_locus_tag="HYQ63_39830"
     CDS             5001..6194
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720316.1"
                     /locus_tag="HYQ63_RS39830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39830"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_257232463.1"
                     /sec_met_domain="DAO (E-value: 2.3e-68, bitscore: 230.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTSSRPGIPRPPDVLVIGAGIIGAACAYYCTSAGLRVTVVERG
                     AIGGGTTGACEGNILVSDKETGPELDLALLSSRLWREIRDDLGAAAVEYHAKGGVVVAG
                     SDATAAGLRQLTERQRTAGVEAVDVRPDELAELEPNLTHEVAGGAHYPQDAQVQPVLAA
                     AHLLRHARARGATVHTGVSVTGFLRDRRGAVTGVRTDSATLPALHARWVVNAAGTWAGQ
                     LADLAGAPVPVLPRKGFILVTEPLTRVVRHKVYTAEYVANVASDDAGLETSVVVEGTRA
                     GTVLIGASRERVGFDRTVALPVLRKLADQAIALFPFLSDVALLRSYLGFRPYCPDHLPV
                     IGPDPRAPGLVHTCGHEGAGIGLAPATGHLIAQLLTDRARDLSLTPFRPERFAEAAA"
     misc_feature    5700..5702
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6191..6472
                     /locus_tag="HYQ63_RS39835"
                     /old_locus_tag="HYQ63_39835"
     CDS             6191..6472
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720317.1"
                     /locus_tag="HYQ63_RS39835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39835"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_179938737.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.2e-27, bitscore: 94.8,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRTPFTFRFDGRTIAAEPGQSIAAALIADGQRSWRRTREGGRPR
                     GMFCGIGVCFDCLVIVNGRPNRRACLVEAAPGDVVETQEGAGRAHLAC"
     gene            6453..7940
                     /locus_tag="HYQ63_RS39840"
                     /old_locus_tag="HYQ63_39840"
     CDS             6453..7940
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378675.1"
                     /locus_tag="HYQ63_RS39840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39840"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_179938739.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5e-24, bitscore:
                     84.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 2.2e-08, bitscore:
                     33.3, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPISPAEPANVPYDVPYDVPYDVAVLGAGPAGLSAATAAAAHGAR
                     VALLDAAPRAGGQYWRHRPADDGAAHHDWPLFQRLRTAADTHRARLDHLPGHAIWHVER
                     TDDGFATHALSDGDGASGVVLSRTVVVATGAYDRQLPFPGWTLPGVYTAGGVQALLKGH
                     GVVAGRRVVVAGTGPFLLPVAAGLAGAGATVLGVFEAGRPTAFARHPLTTLRAAAKLGE
                     AAHYLRTLRRHRIPYRTRTTVVRAEGTDTVTGAVVARLDDRWRIVPGTRRRLECDTVAV
                     GYGFTPQTEIPLQLGCAMHRDADGSLTARVDTRQRSTVPGVYVAGEACGVGGAQLACTE
                     GELAGLHAAYATTGTRPDPRRIARLLRLRAARRGFAAALHAAYPVRPGWREWLTEDTVV
                     CRCEEVTAGAIRQVAEELGATDPRAVKLCARPGMGLCQGRVCGYATAALTAAHAGRDVT
                     EDDLRGLASRPVAQPVPLGLLRNPGTESMPHHDEEGA"
     gene            7937..8845
                     /locus_tag="HYQ63_RS39845"
                     /old_locus_tag="HYQ63_39845"
     CDS             7937..8845
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720319.1"
                     /locus_tag="HYQ63_RS39845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39845"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_179938741.1"
                     /transl_table=11
                     /translation="MNAQAKPWHGVLVATALPLREDPAGRLAVDFDGYAEHLTWLAANG
                     CHGVVPNGSLGEYQTLTAEERARVVTTAVEAAPEGFTVMPGVAAYGADEARRWAEQAAE
                     AGAPAVMLLPPNAYRADERAVLAHYREVARAGLPVVAYNNPIDTKVDLVPELLAELYHE
                     GLIGAVKEFTGDVRRGYRIHELAPGLDVLCGSDDVALELAVAGSPGWIAGYPNAFPRAC
                     AELWRAATAGDLTTALPLYRTLHPLLRWDARTEFVQAIKFSMDIAGRYGGPCRHPRVPL
                     TAEHADTVRKATEKALAEGLV"
     gene            8850..9851
                     /locus_tag="HYQ63_RS39850"
                     /old_locus_tag="HYQ63_39850"
     CDS             8850..9851
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358096.1"
                     /locus_tag="HYQ63_RS39850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39850"
                     /product="proline racemase family protein"
                     /protein_id="WP_179938743.1"
                     /transl_table=11
                     /translation="MRSDRVFHAVDSHTEGMPTRVITGGAGVIPGATMFDRRRYFMAHL
                     DHIRRFLMNEPRGHSAMSGAILQPPTRPDADWGVLFIETSGCLPMCGHGTIGVATVLVE
                     TGMVAVEEPVTTIRLDTPAGLVVVDVAVERGAARGVTLRNVPSFALALDRTVKTAAFGD
                     IGYDMAFGGNFYAIVDLDHLGIPFDRAHKNRIMDAGLAIMAAINEQARPVHPDNPEISG
                     CHHVYLAAPGSDARHSRHAMAIHPGMFDRSPCGTGTSARMAQLHTRGLLPLHTDFRNES
                     FIGTHFTGRLVEETTVAGLPAVVPTVTGRAWITGTAQYLRDPDDPFPEGFEL"
     gene            9862..10839
                     /locus_tag="HYQ63_RS39855"
                     /old_locus_tag="HYQ63_39855"
     CDS             9862..10839
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139737.1"
                     /locus_tag="HYQ63_RS39855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39855"
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="WP_179938745.1"
                     /transl_table=11
                     /translation="MKIIDAASVAAALPMRAAIRALQQALRDGLDPEADPPRAVVPVDH
                     GQLLLMPAEGRSPRGRAYAGVKMATVAPDNPARGLPRIQGQYLLLDAETLTPLALLDGV
                     ALTNLRTAAVSAAAADLLAPPDASRLVLFGGGPQAHSHLAALRAIRPLTEVTVVGRTPE
                     RARQLVEGFPETAGVRVVAGEPDAVADADLIACCTTARTPLFDGTLLPPHATVLAVGSH
                     EPGAREVDDHTVRHATVVVESRAAATREAGDLIQVIDRGLLDPAQLLPLADLIRTTPGG
                     PAAASGRPRLFKSVGMAWEDLVIAGTVHEAHEAGLSGESGEGDR"
     gene            10854..11606
                     /locus_tag="HYQ63_RS39860"
                     /old_locus_tag="HYQ63_39860"
     CDS             10854..11606
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720322.1"
                     /locus_tag="HYQ63_RS39860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39860"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_257232464.1"
                     /transl_table=11
                     /translation="MTVDDGATARLPSLRGRRSLREEIAETLRGAVISGELKPGVVYSA
                     PSLAEHFGVSATPVREAMLDLSKEGLVEIARNKGFRVTHLSAAELDDITHLRALIEIPV
                     IRQITEAGVAPEVIARLRPLAAGIEEAANRRDFIAHVTIDMEFHLTLLEQAGNSRLVET
                     VRSLRTSSRLYGLRTLTEGSALFDSSHEHAELLDLIEAGDADGAEALMRRHLGHVRGIW
                     AEENAPDPDPAPQGRRGGRSRPTGEPPR"
     misc_feature    complement(12022..12036)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.02"
                     /tool="antismash"
     misc_feature    12360..12374
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     misc_feature    complement(12370..12383)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 tctccgcaca cgtacacctc ggcgccctcc gccagcagcc gccagatctc cgcggcgtcg
       61 gcggcgatgc ggtgctggac gaagcggtgg ccgtccaccg gggcctggct gaacgccggg
      121 cgcatggaca ccgcgccgac ggcctcggcg gcgcgcagct cctcgctgtg cagatagtcg
      181 gcgtcgggcg cgtcgcaccc gaagtagcac agcgccgggg cgagcggtgt gccggactcc
      241 acgagcgccc gccggtcggc gacggtgccg cggaaggggg cgagcccggt accggcggac
      301 accatgatga ccggccggtc cggcgcgtgg ccgatgcgga acgcgtcccg gcagggctgc
      361 acccgggcga gcacggtgtc cccgggccgc accgcgttca gatggccgga gccggtgccc
      421 tggaaaccgc tcgtctcgtc gcgcagcagc gacaccatca gatcgacgtg gcgcggtgag
      481 acggcggggg aggacgagat cgagtagtgg cgcggccgca gcggcggcag cagctccagc
      541 agcaccggcc aggtcagcgt ctcgcgcagc gccgggttgg cctcgatcag gtccagcagg
      601 gtgcgttcac cgggggtctg cgcggcgagc gccgccttct ccggcgggca ggggttcagc
      661 tcggcgagca gggccagctg ctcggcgttc ggacggtgat tcagctccac gtggtgcgtc
      721 agcaactccc gtacggtgac cggccggtcc accgccagac cgccgcggcg ggccgggcgg
      781 gcggacacga tgctgagcac ggtctccggg tcggcgtgca gcagcttggt ggcccgcgtc
      841 accagctccg ggtcgttcgc cgggagcacg gtgaggtggt cggcggtgcg gtacgcgacc
      901 ccctcgggca gggccagccg cacgaaccgc ttgacgcgcg gatacgacgg atcgctgagg
      961 ctgccggtct cggtgacctc catcgccacc atgccgtgct gccgcgccag accgtccagc
     1021 ggcccgccgg tgacctcggt gacggtgtac gccgggccgt cgtccgcggc gtcggcggcc
     1081 ggggcagcgc ccacggtggc cgggtcgccg tggcgctcca gcagcgcggt gcgcagccgc
     1141 tcggtgaagt cccgcaccgt gccgctgaga tcgcccgagg cgtcggccgc ggcccggtcg
     1201 atgagccggg tggcgccgag ggcggccagc cgctcgtcga tcagggtcgg cacgcgctgg
     1261 taggtggcgg cccagttgcg gtcgccgacg ccgagcaccg cgtacgcgac gccgtccgcc
     1321 gcgcccggct cggccgtctc cagccaggcc aggaaggcgg cggcgtcatc ggtgggctgc
     1381 ccgttgtagg aggcggcggt gatgaccacc gggcggtcgg tgggcagccc gcccgcgtag
     1441 gcgtcgagcg gggcgacctc ggtggcgcag tccaggccgt ccgcgtcggc ggccagatcc
     1501 tcggcgaagg agcggcaggt gccgaagttg gtgccgtaca gcagcagcgc accggtgtcc
     1561 tggcgcaccc ggctcggcag cgaggcgtcc cgtgcgggcg cgcttgtcgc caccgcggcg
     1621 gccgccgcca ccgcccgctc ctccggggtg cgggggatca gccgcagggt gagcccttcg
     1681 ggcttgaggg tgagcgactc cttgacgtgg agccggtagt ccgcgtggtc gatcagccgg
     1741 tagcggtgca ccagcatcgc gagcagcatg gtcgcctcgt gcagcgcgaa ctgccgcccg
     1801 atacaggccc gttcaccggt gccgaacggc ttgtaagcgt gcaccggacg cgccgcctcg
     1861 gcctccgggt cgaagcgcga cgggtcgaac agctccgggt tgtcgcccca caccgcggcg
     1921 cggtgcagca tcggcgtgag cacgatggcg ggctgcccgg cccgcatcgg aacgcgcccg
     1981 ccgagcatcg tgtcctcgcg cgcctgccgg gagaaggcgg ccgcggtcgg ccacagccgc
     2041 agcgcctcgt tcagcacctg gcgggtgtag cgcaacagcc ccacctgctc gaacgtcggc
     2101 tccgggtccg cgtcggcgcc gaacagcgcg tccgcctccc gctgcacccg gcgcagttcg
     2161 cccgggttct tggcgaggta gtgcagggcg aaggacagcg cgcccgaggt ggtctcgtga
     2221 cccgcgatca ggaaggtgat cacctggttc cggatgttgg cgtggtccag cggggtgccg
     2281 tcggacggat gcgtggcgcc gagcatcagc cccagcagat cgtcggtgcc cgactcgccc
     2341 gcggcgaccc gggcctggat cacctcgtcg acgacggacg tgagatacgc cgtatcggcg
     2401 cggaacgcct cgtcctgctc gctgtagtcc acatccgggc ggcgcgccac cttgcgcatg
     2461 ctccactcca ggcagcgcac catggcgctg acgaacggat gcggttccgc gcggtcgaac
     2521 gagccgaagt cgtagccgaa cccggccagc ccgatggtgt ccagcgtcat ccgcgtcatg
     2581 tcgtccgtca ccgcgaccgg ctcgccgctc gccgcgcggg cgtcccaggc gtcgatcacc
     2641 cgccgcgcca cctggagcat caccgggtgg tacgtgcgca tcgagccgag cgcgaaggcg
     2701 ggcatcagga tgtcgtgcgc cttcgcccag ttcggctcgt cgttgtacgc ggtgaacagc
     2761 ccgtcgtggg tgaactcccg gacgttctcc agcgactggt tgacgaactt ggcgaaccgc
     2821 tggtcgtcac agagttccgc caccaggtcg gggtcgctga cgaacagcac gtcggtgccg
     2881 tgcagccgcc gccgcagcac cgggccatgg cgtcgcgtca gctccatggc ctgctggatc
     2941 ggagtgttgt tcgtctgcgc ggagatgtcg acgagcggga caccgtcgga gctgccgccc
     3001 tcggcggtcc cggccggaag gaggggggtt tcggtcatga cgatgcgatg ccttccatgc
     3061 gaggggatgt ctccagcctc gccccgccga cccgggccgc tcgccgggcg cactacatga
     3121 ttttgtagtg ccgaatggtg cgatcgactt gcgcacgcgc cgttcgcggt gtcaacagga
     3181 gtgcggtgcc aagaggtgtg acgtacgaga gcgaggtccg gcatggacga gcccgaggtg
     3241 ccggaagagg cgaggaagat cctcggcggt gaggactggg tcgtctggcg caaccgctcg
     3301 ctgctgctct tcgaccgggt cccggtgccg tgcgcggtgt gccgcacgga cggcgagatc
     3361 ctgatggcca acccggcgat ggcggccgag tggggcaccg cccccgggca actggcgggg
     3421 cgcaacgtcc tggagctgtt ccaccccaac gcggccgccc aggtgcaccg catcgtcgag
     3481 gcggtccggc tcggacgccg ctcccgctat ccgatcgagg tgcggtggca gtcgccggac
     3541 ggggcgcggc ggtacggcga actgaccgcg gacaccgtga gcgacaccga cagcccgatg
     3601 tggctgctgg tgctgctgcg ggtgctcggc gaggaaccta ggtcgcgggg cgatggcacg
     3661 gcggacccgg cccccgacga gacggagcgg gccgccccca tcgaggcccg gatcctggcg
     3721 ctcgccgcgg gcggccacac caccgcccag atggcccggc agaccggact caccggggac
     3781 ggggtcaact accacctcac ccggctctcc cggcgctggg gcgtcggcaa ccgcgcggag
     3841 ctggtggccc gggcctatgt gctcggcgtg ctgtccccgg gggtctggcc gccggaaccc
     3901 tcctgacacg tcatgccctg acggcgggtc ggtcgcaagg ctcagccgcg catggtcacg
     3961 ccgtggtgcg tacgcagccg ccgcagctcc cacagcgaca gcgcggtcac cgacagcggg
     4021 ccgccgaagg cggcggccag ctgggcgggc agcggcccgt tgggcagccc gtccgacagc
     4081 aggttgaagc ccgcgatcaa ccacaccagc accgtggtga gcacgggtgg cagcgcggcg
     4141 tgcacatagg cggtgttgaa ggcgctgcgc gcgacgtacg cggaggacag gaagaggaac
     4201 accagcgccc acggcagacc caccatcagc ggcaggccga ccgcgaacag catcacgccg
     4261 tgcagcccgt gcagcagcgt cgtctggccc accacataga tgaagacgcc gaaggccacc
     4321 aggatcagca cataccagcc caccgtcttg agcgggccgc gcagccggga gcgcagctcc
     4381 gggcgccggt gcgggggcgc gtagaggatg aagccgaaca cgaccagcgg gccggtgagc
     4441 aggagcagga gcggggtcgc gaagagattg gcggcaccgt ccgtcgccgc accgctccag
     4501 ccgccgtccg ccccgtacat gtagatcagc gccaggctcg cgacgatacc gagaacggag
     4561 cgggtgagct ggacccggtc gacgagatgg tcccggaccc gcccgccgcc ctcctggtcg
     4621 aacagccgct tcgccacgcg gttcgccgaa cggaccagcg ggatgagcgg cgagagcagc
     4681 caccaccgac ggcggcgcac cggggccggc ggatacgggc cgggcggctg cggaggcaca
     4741 ccgtacgggg cgggcggtgg cggtccgtag gggttcggcg gcggcgggcc gtagtacggg
     4801 ctctgcggcg gcggcccgta tggatcgggt gtgggcgacg gaccccacgg tccgccccct
     4861 tgaccaggat tgttcggcgg ctgccatccc cccgtagcca cgtgcactcc tccctcgcta
     4921 ggtgccgtgg cggcgaattc tagccatgtg ccgtcgccgc accctggcca gggatagtgc
     4981 catatgacat gctaccggcc atgaccggta cgagttcccg acccggcatt ccccgccccc
     5041 cggatgtgct cgtcatcggg gcgggcatca tcggcgcggc ctgcgcgtat tactgcacgt
     5101 cagcggggtt gcgcgtgact gttgtggagc gtggcgcgat cggcggcgga accaccggcg
     5161 cgtgcgaggg caacatcctg gtgtccgaca aggagaccgg gcccgaactg gacctggcgc
     5221 tgctgtcctc ccggctgtgg cgggaaatcc gggacgacct cggggccgcc gccgtggagt
     5281 accacgccaa gggcggtgtg gtggtggccg gttcggacgc caccgcggcg gggctgcggc
     5341 agctcaccga gcggcagcgc acggccgggg tcgaggcggt ggacgtccgc ccggacgaac
     5401 tggccgagct ggagcccaac ctgacccacg aggtggccgg cggtgcccac tacccgcagg
     5461 acgcgcaggt ccagcccgtg ctggcggccg cacatctgct gcggcacgcc cgcgcccggg
     5521 gcgcgaccgt ccacaccggg gtgagcgtga ccggcttcct ccgggaccgg cgcggcgccg
     5581 tcaccggggt gcgcaccgac tcggccaccc tgcccgccct gcacgcccga tgggtcgtca
     5641 acgccgcggg tacctgggcc gggcagctcg ccgacctcgc cggggcaccg gtgccggtgt
     5701 taccccgcaa ggggttcatc ctggtcaccg agccgctgac gcgcgtcgta cggcacaagg
     5761 tgtacaccgc cgagtacgtg gccaacgtcg ccagcgacga cgccggtctg gagacctcgg
     5821 tggtggtcga gggcacccgg gcgggcaccg tcctgatcgg cgccagccgg gagcgcgtgg
     5881 gattcgaccg cacggtggcg ctgcccgtgc tgcggaagct cgcggaccag gccatcgctc
     5941 tcttcccgtt cctctccgac gtggcgctgc tccgcagcta tctgggcttc cgcccttact
     6001 gccccgacca cctgccggtg atcgggcccg acccccgtgc cccgggcctc gtccacacct
     6061 gcggccacga gggcgccggg atcggcctgg cccccgccac cggccatctg atcgcccaac
     6121 tgctcaccga ccgggcacgg gatctgtccc tcacaccgtt tcgaccggag cgattcgcgg
     6181 aggccgccgc atgaaccgga ccccgttcac cttccgcttc gacggccgga ccatcgcggc
     6241 cgagcccggc cagagcatcg cggccgcgct catcgccgac gggcagcgct cctggcgccg
     6301 cacccgcgaa ggcggccgcc cgcgcgggat gttctgcggc atcggggtgt gcttcgactg
     6361 cctggtgatc gtcaacgggc gcccgaaccg ccgcgcgtgc ctggtcgagg ccgcgccggg
     6421 ggacgtggtg gagacccagg agggagcggg ccgtgcccat ctcgcctgct gaaccggcga
     6481 acgtcccgta cgacgtcccg tacgacgtcc cgtacgacgt cgcggtactc ggggccgggc
     6541 ccgccgggct gtccgcggcc accgccgcgg cggcccacgg cgcccgggtc gcgctgctgg
     6601 acgcggcgcc ccgcgccggt ggccagtact ggcgccaccg ccccgccgac gacggcgccg
     6661 cgcaccacga ctggcccctc ttccagcggc tgcgcacggc cgcggacacc catcgcgccc
     6721 ggctcgacca cctgcccggc cacgccatct ggcacgtgga gcgcactgat gacggcttcg
     6781 ccacccacgc cctgagcgac ggcgacggcg cgtccggcgt agtcctcagc cgtaccgtcg
     6841 tcgtggccac cggcgcctac gaccgccaat tgcccttccc cggctggacc ctgcccggcg
     6901 tctacaccgc cggtggtgtg caggccctgc tcaaggggca cggcgtcgtc gcgggccggc
     6961 gcgtcgtcgt cgccgggacc gggcccttcc tgctgccggt ggccgcgggc ctcgctgggg
     7021 cgggggccac ggtcctgggc gtcttcgagg ccggacggcc caccgccttc gcccgccacc
     7081 cgctcaccac cctgcgcgcc gccgccaaac tcggcgaggc cgcccactac ctgcggacgc
     7141 tgcggcgcca ccggatcccg taccgaaccc gtaccacggt ggtccgggcc gaggggacgg
     7201 acaccgtcac cggggcggtg gtggcgcggc tggacgaccg gtggcggatc gtgcccggca
     7261 cccggcggcg gctggagtgc gacacggtgg ccgtggggta cggcttcacc ccgcagaccg
     7321 agattccgct ccagctcggc tgcgcgatgc accgggacgc cgacggcagc ctgaccgccc
     7381 gcgtggacac ccggcagcgc tccaccgtgc ccggcgtcta tgtggcgggc gaggcatgcg
     7441 gggtcggcgg agcccaactc gcctgtaccg agggggagct ggcggggctg cacgcggcgt
     7501 acgccaccac cggcacccgc cccgacccgc gcaggatcgc ccggctgctg cggctccggg
     7561 ccgcccggcg cggcttcgcc gcagcgctgc acgcggcgta tccggtgcgg cccgggtggc
     7621 gggagtggct gaccgaggac accgtcgtgt gccgctgcga ggaggtgacc gccggtgcca
     7681 tccgccaggt ggcggaggag ctgggggcca ccgatccccg ggcggtgaag ctgtgtgcgc
     7741 gccccggaat gggcctgtgc cagggccggg tgtgcggcta tgccaccgcc gccctgaccg
     7801 ccgcccacgc cggacgcgac gtcaccgagg acgacctgcg cggtctggcg agccgacccg
     7861 tcgcccagcc ggtaccgctc ggactgctga ggaacccggg tacggaatcc atgccacacc
     7921 acgacgagga gggcgcatga acgcacaggc caagccatgg cacggggtgc tcgtcgcgac
     7981 ggcgttgccg ctgcgcgagg acccggccgg caggctcgcg gtcgacttcg acgggtacgc
     8041 cgaacacctc acctggctcg cggcgaacgg ctgtcacggt gtcgtcccca acggctccct
     8101 cggcgagtac cagaccctga ccgccgagga acgggcccgc gtggtcacca ccgccgtcga
     8161 ggccgccccc gagggcttca ccgtgatgcc cggcgtggcg gcctacggcg ccgacgaggc
     8221 ccggcgctgg gccgagcagg cggccgaggc gggcgccccg gccgtgatgc tgctgccgcc
     8281 caacgcctac cgtgccgacg aacgcgcggt gctggcccac taccgggaag tggccagggc
     8341 cgggctgccc gtcgtggcgt acaacaaccc catcgacacc aaggtcgacc tggtgcccga
     8401 gctgctggcg gagctgtacc acgaggggct gatcggggcg gtgaaggagt tcaccggcga
     8461 tgtccgccgc ggctaccgga tccatgaact ggccccgggg ctcgacgtgt tgtgcggcag
     8521 cgacgacgtg gcgctggaac tggccgtggc cgggtcgccc ggctggatcg ccggctaccc
     8581 caacgccttc ccgcgcgcct gcgccgagct gtggcgggcc gccaccgcgg gcgatctcac
     8641 caccgcgctg ccgctctacc gcaccctgca cccgctgctg cgctgggacg ccaggaccga
     8701 gttcgtccag gcgatcaagt tcagcatgga catcgccggg cgctacggcg gaccctgccg
     8761 ccacccgcgc gtaccgctca ccgccgaaca cgccgacacg gtgcgcaagg ccaccgagaa
     8821 ggccctcgcg gaagggctcg tctgaaccca tgcgcagcga ccgggtcttc cacgccgtcg
     8881 actcgcacac cgaagggatg ccgacccgcg tcatcaccgg cggcgccggg gtgattcccg
     8941 gcgccaccat gttcgaccgg cgacggtact tcatggcaca cctcgaccac atccgccggt
     9001 tcctcatgaa cgagccacgc ggccactcgg cgatgagcgg ggccatcctc cagccgccga
     9061 cccgccccga cgccgactgg ggcgtgctct tcatcgagac ctccggctgc ctgccgatgt
     9121 gcggacacgg caccatcggc gtcgccaccg tcctggtcga aaccggcatg gtggcggtcg
     9181 aggagcccgt caccaccatc cggctggaca cccccgccgg gctggtcgtg gtggacgtgg
     9241 ccgtcgagcg gggcgcggcc cgcggggtga cactgcgcaa cgtgccctcg ttcgcgctcg
     9301 ccctggaccg caccgtgaag accgcggcct tcggcgacat cggctacgac atggccttcg
     9361 gcggaaactt ctacgccatc gtcgacctgg accacctcgg gatccccttc gacagggccc
     9421 acaagaaccg catcatggac gccggactcg ccatcatggc cgccatcaac gagcaggccc
     9481 gcccggtcca ccccgacaac cccgagatca gcggctgcca ccacgtgtat ctggccgccc
     9541 ccggatcgga cgcccggcac tcccggcacg ccatggccat ccacccgggc atgttcgacc
     9601 gctcgccctg cggcaccggc accagcgctc gcatggccca gctgcacacc cgcggactgc
     9661 tgccgctgca caccgacttc cgcaacgaat cgttcatcgg cacccacttc accggccgac
     9721 tggtcgagga gaccacggtc gccggactgc ccgcggtggt gcccaccgtc accgggcgcg
     9781 cctggatcac cggcaccgcc cagtatctgc gcgaccccga cgacccgttc cccgaaggct
     9841 tcgagctgtg aagagccgac catgaagatc atcgacgcgg cctcggtcgc cgccgccctg
     9901 cccatgcgcg ccgcgatccg ggccctccag caggcgctac gggacgggct cgaccccgag
     9961 gccgacccgc cccgcgccgt ggtgccggtg gaccacggcc agctgctgct gatgcccgcc
    10021 gagggccgta gcccccgagg acgcgcctac gcgggcgtca agatggccac cgtcgcaccg
    10081 gacaacccgg cgcgcggact tccccgcatc caggggcagt atctgctgct ggacgccgaa
    10141 accctcaccc cgctcgccct gctggacggg gtcgcgctca ccaacctccg caccgcagcg
    10201 gtgtccgccg cggccgccga cctgctcgcc ccgcccgacg cgtcccggct ggtgctcttc
    10261 ggcggcggcc cgcaggccca cagccatctg gccgcgctgc gcgcgatccg ccccctcacc
    10321 gaggtcaccg tcgtcggccg taccccggag cgggcacgcc aactggtcga gggcttcccg
    10381 gaaaccgccg gggtccgcgt tgtggccggg gagccggacg cggtcgcgga cgccgatctg
    10441 atcgcctgct gcaccacggc ccgcaccccg ctgttcgacg gaaccctgct gcccccgcac
    10501 gccaccgtgc tggccgtggg atcgcacgaa cccggtgcca gggaggtcga cgaccacacc
    10561 gtccgccacg ccaccgtggt ggtcgagtcc cgcgccgccg ccacccggga ggcgggcgac
    10621 ctcatccagg tcatcgaccg cggcctgctc gaccccgccc aactgctgcc cctcgccgac
    10681 ctgatacgca ccactccggg cggcccggcg gcggcctccg ggcgcccccg gctgttcaag
    10741 agcgtgggaa tggcgtggga agatctcgtg atcgccggta ccgtccatga ggcccatgag
    10801 gccggcctgt ccggtgaatc cggcgagggc gacagatgaa ggagaccgac cgcatgaccg
    10861 tggacgacgg ggccaccgcg cgcctgccct cgctccgcgg ccgccgcagc ctccgcgagg
    10921 agatcgccga aaccctccgc ggcgcggtga tctcgggcga actgaagccc ggcgtcgtgt
    10981 actccgcccc gagcctggcc gaacacttcg gagtgtccgc gaccccggtg cgcgaggcca
    11041 tgctcgacct ctccaaggag gggctggtcg agatcgcccg caacaagggc ttccgggtca
    11101 cccacctctc cgcggccgaa ctggacgaca tcacccacct gcgcgcgctc atcgagatcc
    11161 cggtgatccg gcagatcacc gaggcgggcg tcgcccccga ggtcatcgcg cggctgcggc
    11221 cgctggcggc gggcatcgag gaggccgcga accgccgcga cttcatcgcc cacgtcacca
    11281 tcgacatgga attccacctc accctgctgg aacaggccgg gaacagcagg ctggtggaga
    11341 ccgtgcgctc gctgcgcacc agttcccggc tctacggact gcgcacgctg accgaaggca
    11401 gcgccctgtt cgactcctcc cacgaacacg ccgaactcct cgacctcatc gaggcgggcg
    11461 acgcggacgg cgccgaggcc ctgatgcgcc gccacctcgg ccacgtccgc ggcatctggg
    11521 ccgaggagaa cgcccccgac cccgaccccg ccccccaagg ccgccgaggc ggccggagcc
    11581 gcccgaccgg ggagccgccc cggtgagcac ggtgcacgag gccgaggaga cctaccggcg
    11641 gatcgcggcc catgcgtggc gcgcccccac cgacgccgtg ctggccgacc gggtggggtg
    11701 agtcagccgg tggcgaggcc gtggacgatg tccgcggtcg ccgggtacag ctcgcggtag
    11761 cgcgcgtaca gctcgtcgta gcgcgccgtg gtggccgggt cggggcgcac ctggacggcg
    11821 ggcggattcc accgcgcgat ggaggcatcc ccgacggccg acgcggcgag gaacgccgaa
    11881 ccgaggctcg cgccgatggt gtgcgtgggg acgtcctggg tcagcccggt gatgtcggag
    11941 acgatctgcg tccacagatg cccctgggcg ccaccgccca cggccaccac ccggtcgatg
    12001 cggccaccgg cggcgcggat cgcctccacg ttgtggcgga cgccgaaccc ggtggcctcc
    12061 agcgcggcgc ggtagagatc gccgcgggtg tgcgcgaggg tgaggcccgc gaggacaccg
    12121 cgggccgcgg ggtcggcgac gggagtgcgc tccccgtcga agtacggcag catcagcagc
    12181 ccgcgcgccc ccgggcccga gatctccgcc tcggccagca gttcgggcac ggacgggtcc
    12241 ccgaacagcg agcgcagcca gtcggtgatg gcgcccgagg acgccatgcc accggccagg
    12301 ttgcgggtgc cggggagcgc cccgacggtg ccccacaggg cggggaaggt gacgcgctcc
    12361 tcaaccgtgt tgatcaggaa catggtcgag ccgtacatca gcatcagatc gccggcgttg
    12421 tgcgcgtcgc cgctgaccgc ctccgcccag gcgtcgatgg tgccgaccgt caccggggtg
    12481 cccgccgtca gccccgtcgc ctccgccgcc cgcggcgaca cccggcccgc cacgtccccg
    12541 gaccaggcga gccggggcag ttcgatccgc cccgccagcc cgtccgacca ggggcggtac
    12601 cagtcgaggg cgccggtgtc gtagagcggg gtgcactggc tggccgagtg gtggtccagt
    12661 acgtactccc cggtgagccg gtgcaccagc caggagctgg gcatgaacag ccgccgggcg
    12721 cgggcgaaga cgccgggctc gtgccgtgcg agccaggcga gcttggggcc gacggcctgg
    12781 gtggagagca ccgaaccgca gcggcgcagg atctcctcgc ggcccagcgc ggcctccagc
    12841 tcgccgatct gctcggtggc gcgggtgtcg atcccgtaca gcagggccgg gcgcagcggc
    12901 acaccggcgt cgtccgtgac cgcgacacac ggccccatcc
//
