LOCUS       NZ_CP058693            10225 bp    DNA     linear   CON 08-SEP-2024
DEFINITION  Streptomyces sp. Rer75 chromosome.
ACCESSION   NZ_CP058693
VERSION     NZ_CP058693.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Rer75
  ORGANISM  Streptomyces sp. Rer75
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Genome analysis of Streptomyces sp. Rer75
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) College of Pharmacy, Henan University of
            Chinese Medicine, Jinshui East Road No. 156, Zhengzhou, Henan
            450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. Canu V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.63x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013414305.1-RS_2024_09_08
            Annotation Date                   :: 09/08/2024 05:25:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,857
            CDSs (total)                      :: 8,765
            Genes (coding)                    :: 8,460
            CDSs (with protein)               :: 8,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 145 of 305
            Pseudo Genes (incomplete)         :: 204 of 305
            Pseudo Genes (internal stop)      :: 20 of 305
            Pseudo Genes (multiple problems)  :: 62 of 305
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:27:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9260406
            Orig. end                         :: 9270631
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10225
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="36"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10225
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10225
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5225](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5225
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(287..303)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            complement(621..1034)
                     /locus_tag="HYQ63_RS39285"
                     /old_locus_tag="HYQ63_39285"
     CDS             complement(621..1034)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024770.5"
                     /locus_tag="HYQ63_RS39285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39285"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_179938533.1"
                     /transl_table=11
                     /translation="MAALRDAGIRPDRELIRSVAFQTEPARRAAHALLDLWDPPTAFVT
                     GNDLQGLGVIEACAERGLRVPGDVSVVGFDDIDAAGSAAPALTTVRQPFEDMAAETIRV
                     LGAVTGEPDRAPIRLDMPVDLVIRKSTGPARRG"
     gene            1051..1419
                     /locus_tag="HYQ63_RS39290"
                     /old_locus_tag="HYQ63_39290"
     CDS             1051..1419
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF046780.1"
                     /locus_tag="HYQ63_RS39290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39290"
                     /product="hypothetical protein"
                     /protein_id="WP_373689240.1"
                     /transl_table=11
                     /translation="MTDTATTDPERLAKGWEPIVAELAAFAARQDRKILFTEAGYASQR
                     GSTAAPYSWTVSEHAAADEQSAAYQALLTAFDGRSWWAGVCWWMWDDWPDTGETARKLA
                     YTPHGKPAEKVLRRWWRG"
     gene            1467..3617
                     /locus_tag="HYQ63_RS39295"
                     /old_locus_tag="HYQ63_39295"
     CDS             1467..3617
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670854.1"
                     /locus_tag="HYQ63_RS39295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39295"
                     /product="FUSC family protein"
                     /protein_id="WP_179938535.1"
                     /transl_table=11
                     /translation="MTWPRSLMDTVRSGLTIERARLEPLAALRAAAGVAIVIGLSLWTA
                     SPSYAASAAFGAFSAGTSTFQRSWRPRKVIALVAGAGLGLSTFVGYLAMSHLALFLLLL
                     AVWAFLAGMAWAAGPTAGIVATTTVAIMLLTITLPTGVIEALEHAAVIAFGGVAQAALI
                     WLFPIRRWGRHRDALADALAAVADYARRLREDPVAPFDPEPMITARSAAAVTPSQARRR
                     PAALHGPRGLAERVRPALAALADPRVGAPAEGPGRDRARELLDAAADVLDAAARSIRRG
                     APAEVPSATRETLRIDDEGGADETDEGGGDETLEGPARQAAVQLSHLLTEVLEIADSSG
                     RGLGPTSGADEAPPLLRPTVFQLVPIVLRAMRRELRGDSPVFRHALRLAAVACLGYVLG
                     SALPLGHGYWAPVTSVMVMRPDFHRTYSRAVARFAGTLVGVALATGAVQLTHPDAYLSG
                     GLAVIAAALTYLLMRTGYAASQCTSAAYAVLLLGMGGEGWHQTVPDRVLLTLLGGVLAM
                     LAYVAFPAWETPRMPDRLADWLAASGRYAAAVVRGYAVPGELPATDVRRTLLADRTAHA
                     AWNEALDRAKREPVRHRGLTRAQAEEAQAALEAFGQAAMLLETHLPQRGARPVPGAARF
                     ADALAADTERAARHVRERRRPRWDRAKEALVDWEADSTAEAVVQSGAELLVRPLDQLTT
                     AVDRAPLERDTEAARQQRQDAL"
     gene            complement(3614..4441)
                     /locus_tag="HYQ63_RS39300"
                     /old_locus_tag="HYQ63_39300"
     CDS             complement(3614..4441)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720214.1"
                     /locus_tag="HYQ63_RS39300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39300"
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="WP_179938537.1"
                     /transl_table=11
                     /translation="MKIGVSTFVTDQGIGPAALGRALDERGFDSLFIAEHSHIPVERRS
                     PYPGGGELPEIYYRTLDPFVTLSTIAGVTTRLLLGTGVALVAQRDPIITAQEVASLDVV
                     SGGRAVFGVGVGWNREEMENHGTDPSTRGRLVNERLEAIRELWTKEKAEYHGEFVNFDP
                     VYQWPKPVQRPHPPIYVGGGEAAFPRIAAHGDGWLANSVPPDALAPQIERLRQVAGRDV
                     PVTVYAVPDTAEALEGYRRLGVDRVLLYLPTKPEGETLAHLDTMAALAGRFRS"
     misc_feature    4963..4977
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.21"
                     /tool="antismash"
     gene            5001..5225
                     /locus_tag="HYQ63_RS39305"
                     /old_locus_tag="HYQ63_39305"
     CDS             5001..5225
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) lacticin_l"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="HYQ63_RS39305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39305"
                     /product="type A2 lantipeptide"
                     /protein_id="WP_123108315.1"
                     /sec_met_domain="strepbact (E-value: 9.1e-29, bitscore:
                     98.8, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="lacticin_l (E-value: 7.5e-05, bitscore:
                     21.9, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAPKVATQEISDAELDNVAGGLGAVGNALGTVHSVTGPVTGPV
                     TGAVGTVTGTVDGATGLNTSGIVGTVAGL"
     gene            5368..6177
                     /locus_tag="HYQ63_RS39310"
                     /old_locus_tag="HYQ63_39310"
     CDS             5368..6177
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="HYQ63_RS39310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39310"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_179938539.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSADDIDLPVRYARPQGWLALTVTVVAMAAAAVW
                     AVTGTVGDTLGASGILTHGQGSYVLQSPVRGQVTAVLAEEGSTVRAGAPLVKVRNPGGG
                     ATVVRALAAGRLTTLSVSIGSVLDTGADVASVERVRTSRDPLTATLYVPADRAASVPAG
                     ATVDLDVQSAPSGTYGVLRGHVQAVGRTAQSRQQIGSFLGSAQLGEEFSKQGPPVAVAV
                     RLDRSSATRSGYRWSSPKGPPFALGSMTLASGTIHLADRHPVDWLLP"
     gene            6207..8504
                     /locus_tag="HYQ63_RS39315"
                     /old_locus_tag="HYQ63_39315"
     CDS             6207..8504
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640763.1"
                     /locus_tag="HYQ63_RS39315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_39315"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_257232446.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.8e-37, bitscore:
                     126.5, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADATNGSGGPPRPAPAGTSGRRRPEPARGRRRAPRRGNGPKKPG
                     RRTVRTPTVLQMEALECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNVLKAAR
                     SYGLRAKGMQMEPEALAEVQAPAILFWEFNHYVVYDGMGRRFGKRGVHINDPDKGRRFV
                     SAEEFGSSFTGICLVFEPDDSFRRGGRKPGILNALPARLRGTGGTLLASLLASLLLVAV
                     GAAVPALSRTFIDTFIVGGQTSMLPPLFASMAVAVVLTAVLTGVQQANLLRGRIISSTL
                     GSARFLRHLLRLPVTFFAQRSPADLVQRLRSNDTVAETLARDLAAAAVDGIVVVLYALL
                     LWTYDPQLTLVGVGLALLNVVAMRIVIRLRATRTQKLRADTARLTTTAYTGLQLIETMK
                     ATGGESGYFRRWAGQHAITLEEQQRLGVPSAVLGVVAPTLATLNSALILWIGGLRAVEG
                     HLSVGLLVAFQALVTRFTAPITRLNGVAGRIQDFSADVARLGDVERFPADTLHTRDEGQ
                     PDADGARRLTGHVTLEDITFGYNPLDEPLLRGFSLSVGPGRQVALVGGSGSGKSTVSRL
                     VSGLYSPWEGAIRIDGQRLEDIPRGRLAASVSFVDQDVFLFEGTVRDNVALWDPSIPDD
                     AVVAALRDAALYDDVIARRPGGIHCRVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDE
                     VTSALDAQTEQTVMDSLRRRGCACVVIAHRLSTVRDSDEILVLDRGTVVERGRHEDLAV
                     AGGPYAELMKEH"
ORIGIN
        1 gggcacgtgg aacgacaagg agcacgactt cgacgccatc cagatggacg gcgcccacca
       61 tgtgtggatc gatcacaacg acctgaagca catggccgac ggcctgatcg acagccgcaa
      121 ggacacgacg tacctcacgg tctcctggaa ccggctgcaa acgcacaaca agacgttcgg
      181 catcggctgg acggagaaca ccacggccga catcaccatc caccacaact ggttccggga
      241 gacggagcag cgcaacccgt ccacggacaa cgtcgcgcac gcgcacctgt acaacaacta
      301 cctccaggac gccccgggga cggacatcgc ctcctcctac ggcaactacg cgcgcggcaa
      361 caccaagatg gtgctggaga acagctactt cgaggggatg aagaaccccg tcatcaagga
      421 cgccaccgcc tcgctcgtcc agcggggcaa cacgttcgtg ggcaccagcg gccgcaacga
      481 gagcggcggc accgccttcg accccaagac gtactacccc tacactctcg acgccaccgc
      541 caaggtgccg tcggtggtga agtccggatc cggacccggc ggcaccccgg cgcggtagcg
      601 ggccgcggtc tgcgaaagtg tcagccccgg cgggccgggc ccgtggactt cctgatgacg
      661 aggtcgaccg gcatgtcgag ccggatcggg gcccggtccg gctcaccggt gaccgcgccg
      721 agcacccgga tggtctcggc cgccatgtcc tcgaagggct gacgcaccgt ggtcagcgcg
      781 ggcgcggcgg acccggcggc gtcgatgtcg tcgaacccga cgaccgagac gtctccgggg
      841 acgcgcagcc cgcgttcggc acacgcctcg atgacgccga gcccctggag gtcgttgccg
      901 gtgacgaagg ccgtcggggg atcccacagg tcgaggaggg cgtgggcggc gcgccgggcg
      961 ggttcggtct ggaacgccac cgaccggatc agttcgcggt ccgggcggat ccccgcgtcc
     1021 cggagcgcgg ccatcgacgc ctactggccg ctgacggaca ccgccaccac cgacccggag
     1081 cggctggcga agggctggga gccgatcgtg gccgaactcg ccgcgttcgc cgcccggcag
     1141 gaccggaaga tcctgttcac cgaggccgga tacgccagcc agcgcggcag cacggccgcc
     1201 ccgtactcct ggaccgtcag cgagcacgcc gcggccgacg aacagtccgc cgcgtaccag
     1261 gccctgctga ccgccttcga cggccggtcc tggtgggccg gggtgtgctg gtggatgtgg
     1321 gacgactggc cggacaccgg cgagaccgcg cgaaagctcg cctacacccc gcacggcaaa
     1381 cccgccgaga aggtgctgcg ccgctggtgg cgggggtgag acccggctga cgtgcgcccc
     1441 cgccacttcc gcctaggctc ggctgtgtga cgtggccgcg gtcgctgatg gacaccgtgc
     1501 gttccgggct gacgatcgag cgtgcccggc tcgaaccgct ggccgcgctg cgcgcggcgg
     1561 ccggagtggc gatcgtgatc gggctctcgc tgtggaccgc ctccccgtcg tacgccgcgt
     1621 ccgccgcgtt cggcgccttc tcggccggta cgtcgacgtt ccagcgcagc tggcggccgc
     1681 gcaaggtgat cgcgctggtc gcgggggccg ggctcgggct cagcaccttc gtgggctatc
     1741 tcgcgatgtc ccatctggcg ctcttcctgc tgctgctcgc cgtctgggcg ttcctcgccg
     1801 ggatggcctg ggcggcgggg ccgaccgccg ggatcgtggc gacgacgacg gtcgccatca
     1861 tgctgctcac catcacgctg cccaccggtg tcatcgaggc gctggaacac gcggcggtca
     1921 tcgcgttcgg cggggtggcg caggcggcgc tgatctggct gttcccgatc cgccgatggg
     1981 ggaggcaccg ggacgcgctg gccgacgccc tggccgcggt ggcggactac gcgcggcggc
     2041 tgcgggagga tccggtggcg ccgttcgacc cggagccgat gatcacggcc cggagcgccg
     2101 cggccgtcac gccctcgcag gcacgccgcc gccctgccgc cctgcacggc ccgcgggggc
     2161 tggccgaacg cgtccgtccc gccctcgcgg ccctcgccga cccccgggtc ggcgcgcccg
     2221 ccgaggggcc gggccgggac cgggcgcggg agctgctcga cgcggccgcc gacgtcctgg
     2281 acgccgccgc ccggtcgatc cgccgcggtg cccccgccga ggtgccgtcc gcgaccaggg
     2341 agaccctgcg gatcgacgac gagggcggcg cggacgagac cgacgagggc ggcggggacg
     2401 agaccctgga ggggcccgcg cgccaagccg ccgtccagct gtcgcacctg ctcaccgagg
     2461 tgctggagat cgccgacagc agcggcaggg ggctgggccc cacgtccggc gcggacgagg
     2521 caccgccgct gctgcggccg acggtgttcc agctggtgcc gatcgtgctg cgcgcgatgc
     2581 gccgcgagct gcgcggcgac tcgcccgtct tccggcacgc cctgcggctc gccgcggtgg
     2641 cctgtctcgg ctatgtgctc ggcagcgcgc ttcccctggg ccatggctac tgggccccgg
     2701 tcacctccgt gatggtgatg cggccggact tccaccgcac ctactcccgg gccgtggccc
     2761 gcttcgcggg cacgctggtg ggcgtcgcgc tggccaccgg cgcggtgcag ctgacgcacc
     2821 cggacgcgta tctgtccggc gggctggccg tgatcgccgc cgccctgacg tatctgctga
     2881 tgcgcaccgg atacgcggcc tcgcagtgca cctcggccgc gtacgccgtc ctcctgctcg
     2941 ggatgggcgg cgaggggtgg caccagacgg tgccggaccg ggtcctgctc accctgctgg
     3001 gcggggtgct cgcgatgctc gcgtacgtgg cgttcccggc ctgggagacc ccgcggatgc
     3061 cggaccggct cgcggactgg ctcgccgcga gcggccggta cgcggcggcg gtggtccgcg
     3121 gctacgccgt ccccggcgag ctgcccgcca cggacgtacg gcgcaccctg ctcgccgacc
     3181 gtacggcgca cgccgcgtgg aacgaggccc tcgaccgggc caagcgggag cccgtgcgcc
     3241 accgggggct gacccgtgcg caggcggagg aggcgcaggc ggcgctggag gcgttcgggc
     3301 aggcggcgat gttgctggaa acccatctgc cgcagcgcgg cgcacggccg gtgcccgggg
     3361 ccgcccggtt cgccgacgcg ctggcggcgg acaccgagcg ggcggcccgc catgtgcggg
     3421 aacggcgcag gccgcgctgg gaccgggcca aggaggccct cgtcgactgg gaagccgaca
     3481 gcaccgcgga agcggtggtc cagagcgggg cggagctgct cgtacggccc ctggaccagc
     3541 tgacgaccgc cgtggaccgg gcgcccctgg aacgggacac cgaggcggcc cggcagcagc
     3601 ggcaagacgc cctctagcta cggaaccgtc cggcgagtgc ggccatcgtg tcgaggtggg
     3661 cgagcgtctc gccctcgggc ttcgtcggca ggtacagcag cacccggtcc acgccgagcc
     3721 gccgataccc ctccagcgcc tcggcggtgt cgggcaccgc gtacaccgtc accggcacgt
     3781 cccggcccgc cacctgccgc agccgctcga tctgcggggc cagcgcgtcg ggcggcacgc
     3841 tgttggccag ccagccgtcg ccgtgcgcgg cgatccgggg gaacgcggcc tcgccgccgc
     3901 ccacgtagat cggcggatgc ggacgctgta cgggcttggg ccactggtag acggggtcga
     3961 agttcacgaa ctcgccgtgg tactccgcct tctccttcgt ccacagctca cggatcgcct
     4021 ccagccgctc gttcaccaga cggccacgcg tggacgggtc ggtcccgtgg ttctccatct
     4081 cctcgcggtt ccatccgacg cccaccccga agacggcccg cccgccggag accacgtcga
     4141 gggaggccac ctcctgcgcc gtgatgatcg ggtcccgctg tgccaccagg gcgaccccgg
     4201 ttcccagcag cagccgtgtg gtcaccccgg cgatggtgct cagcgtgacg aaggggtcga
     4261 gggtgcggta gtagatctcc ggcagctcgc ccccgcccgg gtagggcgag cggcgttcca
     4321 ccgggatgtg gctgtgctcg gcgatgaaga gtgagtcgaa cccccgctcg tcgagggcgc
     4381 gccccaaggc ggcgggtccg atgccctggt cggtcacgaa ggtcgatacg ccaatcttca
     4441 cggcggctcc tcggtcggcg actccacgtt cggcatgcgc gtaggcataa aggtggcgag
     4501 cgggtacccc cggggccgcc cgtcacacgt gacggatcga cgagttgccg acggccgcgg
     4561 aatgcgaccg gaggtcggca ggtcttcaag cgatcggcac atccattcca cagaagtcgc
     4621 gtgtaccgca cgccggcggc gccccgcgat cacggtgtgc ggcggccgcg cccgggaatg
     4681 cgcggttttc cgcgcggagg tggagggcgg cgccgcgcga gtggtgacgc tcgtgtgtgg
     4741 aagcggtgcc ggtcacgtgg gcatgtgtgc acgccatgtg atcggaaagt tggcggaatg
     4801 cgtagttccg gatgcgccgc gattcgaaga cggccagctc acgtggttac atccttccgc
     4861 gccgagacgt tcccggggag ccctctttcg ggaagcgcgg gaacgtgccg cggtcggccg
     4921 acgtgggagc cggatgcttt tcggcaacca ccggccgtta caagctcatt tcgagatacg
     4981 tatccaaggg agacgcagta atgaacaacg cacccaaggt cgcgacccag gagatctccg
     5041 acgctgagct ggacaacgtg gccggtggtc tcggcgctgt gggcaacgcc ctgggcaccg
     5101 tgcactccgt gaccggtccc gtcaccggcc cggtcaccgg cgccgtcggc accgtcaccg
     5161 gcaccgtcga cggtgcgacc ggcctgaaca cctccggcat cgtcggcacg gtcgcgggtc
     5221 tctgagacga ccccgtttgc accccatgcc ccgggcctgc ctggtccggg gcatgcgcgg
     5281 tccccggcgc ccgtcgtacg ggccgcccgg gccgcgcacc cgacccgttc cctcgcgccc
     5341 cctccggccg gttgaggaaa gactcccgtg cagttccgcc agcaggccct ttccaagctc
     5401 cagtcggcag acgacatcga cctgccggtc cgctacgccc ggccccaggg ctggctcgcg
     5461 ctgacggtca ccgtcgtcgc catggccgcg gccgccgtct gggccgtgac cggcaccgtg
     5521 ggggacaccc tgggcgcctc cggcatcctc acccacggcc agggcagcta cgtcctccag
     5581 agcccggtca ggggacaggt caccgccgtg ctcgccgagg agggcagtac ggtgcgggcg
     5641 ggcgcaccgc tggtgaaggt ccgtaacccc ggcggaggag ccacggtcgt gcgcgccctc
     5701 gccgccggcc ggctcaccac cctgtccgtc tcgatcggct ccgtcctcga caccggcgcg
     5761 gacgtcgcct ccgtggagcg cgtccgcacc tcccgcgacc cgctgaccgc gaccctgtac
     5821 gtaccggcgg accgggcggc atcggtgccc gcgggcgcca cggtcgacct cgatgtgcag
     5881 tcggcgccct ccgggacgta cggcgtgctg cgcggccatg tccaggcggt cggccggacg
     5941 gcccagtcgc gccagcagat cggctccttc ctcggcagcg cgcagctcgg cgaggagttc
     6001 tcgaagcagg gcccgccggt cgcggtggcg gtacgcctcg accgttcctc cgccacccgc
     6061 tccggctacc gctggtcctc gccgaagggc ccgccgttcg ccctcggctc catgaccctg
     6121 gcctccggca ccatccacct ggccgaccgt caccccgtcg attggctgct cccgtgaccg
     6181 cgacgaaccc gacgaatccg ccgaacatgg ctgacgcgac gaacgggagc ggcggaccgc
     6241 cccgccccgc acccgccggg accagcggac gccgccgccc cgaaccggcc cgcggacggc
     6301 ggcgcgcacc gcggcgcggg aacgggccga agaagccggg gcgtcggacc gtacgcaccc
     6361 ccaccgtgct ccagatggag gcgctggagt gcggtgccgc gtcgctcgcc atggtgctgg
     6421 cccactacgg acgccacgta ccgctggagg agctgcggat cgcctgcggc gtctcccgcg
     6481 acggatcgcg cgccagcaac gtgctgaagg ccgcccgcag ttacggcctg cgcgccaagg
     6541 gcatgcagat ggagccggag gcgctggccg aggtccaggc cccggcgatc ctgttctggg
     6601 agttcaacca ctacgtcgtc tacgacggca tgggacggcg gttcggcaag cgcggcgtcc
     6661 acatcaacga tcccgacaag gggcgccgtt tcgtctccgc cgaggagttc ggatcgagct
     6721 tcaccgggat ctgcctggtc ttcgagcccg acgactcctt ccgccgcggc ggaaggaagc
     6781 ccggcatcct gaacgccctg cccgcgcggc tgcgcggcac cggcggcacc ctgctggcct
     6841 cgctgctggc cagcctgctg ctggtggcgg tgggcgccgc ggtgcccgcg ctcagccgca
     6901 ccttcatcga caccttcatc gtcggcggcc agacctcgat gctgccaccg ctgttcgcgt
     6961 ccatggccgt ggcggtggtg ctgaccgcgg tgctgaccgg tgtccagcag gcgaatctgc
     7021 tgcgcggacg catcatctcc tccaccctcg gcagcgcccg gttcctgcgc catctgctgc
     7081 ggctgcccgt cacgttcttc gcccagcgca gccccgccga cctggtgcag cgcctgcggt
     7141 ccaacgacac cgtcgccgaa accctcgccc gcgacctggc cgcggcggcg gtcgacggga
     7201 tcgtggtggt gctgtacgcc ctgctgctgt ggacctacga cccccagctc accctggtgg
     7261 gcgtcgggct cgcgctgctc aacgtggtcg cgatgcgcat cgtgatacgg ctgcgcgcca
     7321 ccagaaccca gaagctgcgc gccgacaccg cgcggctgac caccaccgcg tacaccgggc
     7381 tccagctcat cgagaccatg aaggccaccg ggggcgagag cggctacttc cgccgctggg
     7441 ccggtcagca cgccatcacg ctggaggaac agcagcgcct cggtgtgccg agcgcggtcc
     7501 tgggcgtcgt ggcgcccacc ctcgccaccc tcaacagcgc gctgatcctg tggatcggcg
     7561 ggctgcgggc cgtcgagggc catctgtcgg tcgggctgct ggtggccttc caggcgctgg
     7621 tcacccgctt caccgcgccc atcacccggc tgaacggggt ggccgggcgc atccaggact
     7681 tctccgcgga cgtggcccgg ctgggggacg tggagcgctt ccccgcggac accctccaca
     7741 cccgcgacga ggggcagccg gacgccgacg gcgcccgccg cctcaccggc catgtgaccc
     7801 tggaggacat caccttcggc tacaacccgc tggacgaacc gctgctgcgc ggcttctcgc
     7861 tgtccgtcgg ccccggccgc caggtggcgc tggtcggcgg ctccggcagc ggcaagtcca
     7921 ccgtgtcccg gctggtctcc ggcctctaca gcccgtggga gggggccatc cgcatcgacg
     7981 ggcagcggct ggaggacatc ccccgcggcc ggctcgccgc ctcggtgtcc ttcgtcgacc
     8041 aggacgtctt cctgttcgag gggacggtgc gggacaacgt ggcgctgtgg gacccgtcga
     8101 tcccggacga cgcggtggtg gccgccctgc gggacgccgc cctctacgac gacgtcatcg
     8161 cccgccgccc cggtgggatc cactgccggg tcgagcagga cggccgcaac ttctcgggcg
     8221 gacagcggca gcggctggag atcgcccggg ccctggtccg ccgccccagc gtgctggtcc
     8281 tggacgaggt gaccagcgcc ctggacgcgc agaccgaaca gaccgtcatg gacagcctgc
     8341 gccgtcgcgg ctgcgcgtgc gtggtgatcg cccaccggct cagcaccgtc cgggacagcg
     8401 acgagatcct cgtcctggac cgcggcacgg tcgtggaacg cggacggcac gaggacctcg
     8461 cggtcgccgg aggcccgtac gccgagctga tgaaggagca ctgacatggc gtccgtacac
     8521 ccgcccgcgg ccgtggacgc cgacggcggc gacgcggtgg tgcgggcgct gggcgccctc
     8581 ggcaccccgg tcgacggcgc cgggctgcgc agcctcccgc tggagggccc gcaggtgctc
     8641 tggctcgtcg tcgcgggcgc cctggacctg ttcgcggtcg acgccgccga ggaggggcag
     8701 tggcacttcc tcggccgcct ggagacgggc gccgcgctcc ccgggcccgt cgagggcccg
     8761 cgccacaccc tgcacgggcg gccctcgcag gactgcgcgc tgcgccgcat cccgctgcgc
     8821 gagctgtacc ggccggagcg gtacgacgcg tacggcaccc cgtacgacac gtcctacgac
     8881 gccaacgaca cctacgccga cggcaccgcg ttccaggacc cggcgccgac gccgctggaa
     8941 cacgccgtgg cgctcggcat cagccgcagc ctcggcgtgc tgttccaggc cccgctggac
     9001 gggggagcgc cgcccggaga ggaggcggcc gacgaggacc tgctgtggct cccggtgccg
     9061 cccggcagcg tgtgctacgg cgccgagtac agcgcccagg ccgccggaga cctgctgatc
     9121 gacggcgcgc tgtggcagcg gatggtcaac cagcagtacc ggctcggcac cgccgtggac
     9181 cgctggatcg acaccctgga acgggcccac gccgaccgga cggccgccgg tatccgggcc
     9241 ggtgaggccg tacgctcgcg cgccgacgag gcgctcatcg cgtccatcgg aggcccgcgg
     9301 cgctcgcccg gggccgcgga cgacaccgcc gacgacgccg tgcacgcggt ctgccgggcg
     9361 gtggcggacg ccgccgggat caccctcgcc gaaccgccca agggccccgc ccccgacgac
     9421 cgtacggacc ccgtggagcg catcgcggcc cacgcgcgca tccgcacccg cgccgtccgg
     9481 ctggagggcc gctggtggcg cgaggacgcc ggaccgctgg tgggctaccg cgccctgtcc
     9541 ggggcgccgg tggcgctgct gtggcggcgc ggccggtacg aggccgtcaa cccggcctcc
     9601 ggcctgcgga tacggatcgg ccgcggcacc gcggaggagt tcgagccgcg ggcggtgatg
     9661 ttctaccgcc cgctgcccga gcggccgatg acgatgtggc ggctgctgcg cttcacggcc
     9721 cgcggcgccg cgccggacct gcgcagcctg ttcgccgccg gactggtgac ggtggcgctg
     9781 ggcgcgctgg tgccgatcgc caccgggcag gtgctcggcg agttcgtacc ccgcgccgag
     9841 aagagcccca tcgtccagat cgccctggcg ctgatcgtct ccagtgtcgt cgccgcgtcc
     9901 ttcctgttgc tccagaacct gaccctgctg cggctggagg gacggctgga gagcacgctc
     9961 cagcccgccg tctgggaccg gctgctgcgg ctgcccacca cgttcttcgc cgagcgctcc
    10021 accggcgaac tcgccagcgc ggccatgggc atcagcacca tgcgccgaat cctgtccggc
    10081 accgccccgg tggtgctcca ggcgggcacc gtcggggcga tgaacgtcgc cctgctgctg
    10141 ttcttcagcg tgccgctcgc gctcgccgtg atgggcatgc tgctggtcgt ctgcggcatc
    10201 ttcttcgcca tgggcctgtg gcagg
//
