LOCUS       NZ_CP058693            11305 bp    DNA     linear   CON 08-SEP-2024
DEFINITION  Streptomyces sp. Rer75 chromosome.
ACCESSION   NZ_CP058693
VERSION     NZ_CP058693.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Rer75
  ORGANISM  Streptomyces sp. Rer75
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Genome analysis of Streptomyces sp. Rer75
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) College of Pharmacy, Henan University of
            Chinese Medicine, Jinshui East Road No. 156, Zhengzhou, Henan
            450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. Canu V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.63x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013414305.1-RS_2024_09_08
            Annotation Date                   :: 09/08/2024 05:25:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,857
            CDSs (total)                      :: 8,765
            Genes (coding)                    :: 8,460
            CDSs (with protein)               :: 8,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 145 of 305
            Pseudo Genes (incomplete)         :: 204 of 305
            Pseudo Genes (internal stop)      :: 20 of 305
            Pseudo Genes (multiple problems)  :: 62 of 305
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:27:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7405307
            Orig. end                         :: 7416612
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11305
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="27"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11305
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11305
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6305](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6305)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(353..366)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     misc_feature    471..486
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.37"
                     /tool="antismash"
     misc_feature    complement(585..599)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            1429..2178
                     /gene="hemQ"
                     /locus_tag="HYQ63_RS31885"
                     /old_locus_tag="HYQ63_31885"
     CDS             1429..2178
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714421.1"
                     /locus_tag="HYQ63_RS31885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_31885"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_179937256.1"
                     /transl_table=11
                     /translation="MTAAPETPDTPQSVPSPKAPNAGKKAKDLNEVIRYTLWSVFRLRD
                     VLPEDRTGYADEVEQLFAQLAAKDVTVRGTYDVSGLRADADVMIWWHSETSDALQEAYN
                     LFRRTRLGRALEPVWSNMALHRPAEFNKSHIPAFLADENARDYVSVYPFVRSYEWYLLP
                     DEERRTMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASE
                     ARRHVREEVPFYTGRRKPVSELVAGLA"
     gene            complement(2197..3984)
                     /locus_tag="HYQ63_RS31890"
                     /old_locus_tag="HYQ63_31890"
     CDS             complement(2197..3984)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (155-533). E-value:
                     8.1e-18. Score: 56.8. Matches aSDomain:
                     nrpspksdomains_HYQ63_RS31890_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055539.1"
                     /locus_tag="HYQ63_RS31890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_31890"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_179937257.1"
                     /sec_met_domain="PF00561 (E-value: 2.7e-22, bitscore: 78.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYGILGPLSLSLAMTALLATPATGRPLAAPPPTHPTAVAA
                     AGPPAPTAVAAAAPAWTASFGSPASAVLASPALAVPRGGAAEARGVIVAAREAAARGIA
                     FGRCPSSEELSSDVRCGTVSVPLNYARPHGKHIRLTVSRVRASGPKARRQGALVYNPGG
                     PGASGMTFPEYAAEDGFRKTAAAYDFVGYAPRGVGRSAPVSCQRPAAFAKTPTSSPQHP
                     SAAYLRAMVARARAYARGCVRRTGAALRHITTLNNARDLDVLRAALGEKRLTFVGASYG
                     TYYGAVYATLFPRHVRRMVFDSVVNPGPWQIWYRNNLDQSLAFERRWADWRRWAARHHA
                     VYHLGATPARVLAAYRKAAHRLARKPAGGRVGTGELQDAFVRAGYNDAYWDPSARALAA
                     YLRGNPRPLIALAASESGDAVADENANAVYTAVECNDAPWPRRWASWNRENTALARRAP
                     FETWDNVAMNLPCAYWPLGHDTPMDVTARPGLPPVLLLAAERDAATPYAGAKALWRRLP
                     GASLVTERKAGTHGLWGGPNACVNRKVDTYLLTGRTPGRTASCAPRPAPEPRPAPVPES
                     GKQPALPGGPGGSGAPGMP"
     aSDomain        complement(2386..3519)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HYQ63_RS31890_Abhydrolase_1.1"
                     /evalue="8.10E-18"
                     /label="HYQ63_RS31890_Abhydrolase_1.1"
                     /locus_tag="HYQ63_RS31890"
                     /protein_end="533"
                     /protein_start="155"
                     /score="56.8"
                     /tool="antismash"
                     /translation="ALVYNPGGPGASGMTFPEYAAEDGFRKTAAAYDFVGYAPRGVGRS
                     APVSCQRPAAFAKTPTSSPQHPSAAYLRAMVARARAYARGCVRRTGAALRHITTLNNAR
                     DLDVLRAALGEKRLTFVGASYGTYYGAVYATLFPRHVRRMVFDSVVNPGPWQIWYRNNL
                     DQSLAFERRWADWRRWAARHHAVYHLGATPARVLAAYRKAAHRLARKPAGGRVGTGELQ
                     DAFVRAGYNDAYWDPSARALAAYLRGNPRPLIALAASESGDAVADENANAVYTAVECND
                     APWPRRWASWNRENTALARRAPFETWDNVAMNLPCAYWPLGHDTPMDVTARPGLPPVLL
                     LAAERDAATPYAGAKALWRRLPGASLVTERKAGTHGLW"
     gene            complement(4093..4905)
                     /locus_tag="HYQ63_RS31895"
                     /old_locus_tag="HYQ63_31895"
     CDS             complement(4093..4905)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055540.1"
                     /locus_tag="HYQ63_RS31895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_31895"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_179937258.1"
                     /transl_table=11
                     /translation="MLWVLFLLVAWGSAVVSCTRLCLAAVAAAQPMEATAGPRSDGRPL
                     SLYEAAFLAGGPRRVVDLALVSMSRERRLLLAHTGWVTVVDPDGRDDLERSVIAAIGPR
                     GQSPVPPVRTALATADPVRALADRLVAAGLAVPAGARANVAAAVRQVRVACGVVLLTGV
                     IALLMVPPASGPGPALAWFALPLLLTASCLVIARVEVHPYSRWASPAGQQLLSRIDVPP
                     RRPVAQRTGGGADEEPDDGELLTALAVHGTAALPDPALRAALRAHWGH"
     gene            complement(5001..6305)
                     /locus_tag="HYQ63_RS31900"
                     /old_locus_tag="HYQ63_31900"
     CDS             complement(5001..6305)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055542.1"
                     /locus_tag="HYQ63_RS31900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_31900"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_179937259.1"
                     /sec_met_domain="DUF692 (E-value: 2e-95, bitscore: 318.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRLGTGIGWRPEIAEDIARLPGIDWVEVVAENVCPGHVPDALLR
                     LRERGTTVVPHGVSLGLGGAERPDPERLSALAERAEALGSPLVTEHIAFVRAGGPLAGS
                     PAIEAGHLLPVPRTRDALDVLCENVAMAQEALPVPLALENIAALISWPGEEMTEGQFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPTEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAELCARTTPPGVLLERDEDFPAAEELAGELDAIRAVTAAGVTAADAG
                     TTSKHVPNGNDIPDIDGARERLARAQTALLSALVAGAPAPEGFDRQRLGVQSRALTSKR
                     AGVVAKVAPELPEILGDGYRRAFEGYADGRPMTGGYRRDAMAFVEHLLAAGDVRDAATR
                     RRLTLWWQERSGPAPLSSHPLARFARRARLVLSGG"
     misc_feature    complement(6516..6532)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.29"
                     /tool="antismash"
     gene            6667..8700
                     /locus_tag="HYQ63_RS31905"
                     /old_locus_tag="HYQ63_31905"
     CDS             6667..8700
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714416.1"
                     /locus_tag="HYQ63_RS31905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_31905"
                     /product="transglycosylase domain-containing protein"
                     /protein_id="WP_257232674.1"
                     /transl_table=11
                     /translation="MGKHRRNAHRKPPPSSAGRRLQLRRWAARHIRIDYPRAGRRGARR
                     WLPSWRQMGLLFVFCLGSLTGVLSFLYMQTEIPKNLNDFATQQDNVYYWADGSEMARTG
                     PVNRQEVALSKVPEKVQWAILAAENASFYSDSGISPSGLMRAVTRMVTGGETQGGSTIT
                     QQYVKNAYLNQRQTFSRKLTEMFMAIKLDDRMSKQEILERYLNTSWFGRGTYGIQRASY
                     AYYGKDVSRLNASEGAMLASLLKGAGTFDPTLSAKNHTRAVERWKWVLDRMVDIGKLSK
                     AERAKYTTFPDPHPAPRPAGLTGQVGYLVETAKAYVGSHTDISDADFDLGGYQIHTTFE
                     KSKVKALAASVKKTRDGLDPKNRREDRYVRIGAASVTPGGRIEALYGGPGYLEQGFNDA
                     NASVVPAGTAFTPFVYAAALRDGVLKERNSPRTPVAPTTLYDGNNKVAVKTPEGPYWDR
                     SGKIVRGENDGEQSYGQVSLRQAIVQSINTPMMQLGMDVGLDRVRQASIDAGLLPSSFG
                     ARVPMFSLGTATPSSIRMASAYATFTANGTHVPPFSVSGLTRNGEKFALRKDRAKRAFS
                     AQVAAQVDDALHGAVQGGTGQAAAVVGPDTAGKTGTAQDGTSSWFVGYTDEVSTAVSLS
                     RVDPKTQELLPLKGMGGDATGKASDTYPLDIWTRYMAGAGKK"
     gene            complement(8778..10028)
                     /locus_tag="HYQ63_RS31910"
                     /old_locus_tag="HYQ63_31910"
     CDS             complement(8778..10028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175673.1"
                     /locus_tag="HYQ63_RS31910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_31910"
                     /product="hypothetical protein"
                     /protein_id="WP_257232272.1"
                     /transl_table=11
                     /translation="MAAPLGALTAVAVVVALLIALTSPGDTSGAVDGSVQAAAPVPRAD
                     KAPRGTKKKNKYADWDGKVKVIGDGSTSYTGPQPKQPKIEKLKPGQKPPQFVVFSWDGA
                     LENGDHLFSRFRQVAKESDAQMTFFLSGMYLLPKYKSSLYRPPMHKQGAAAISFPTDEH
                     IRETLEQLRGAWLEGNEIGSHFNGHFCGSKGGGEWTTRQWKSETEQAYSFVKNWKTNTG
                     FTDIEPLPFDYDEELVGGRAPCLEGQKNLIPAIKSMGWRYDASSPGDFQIWPSKIDGIW
                     NFPLQLVPYPESEKQVLSMDFNFLFNQSGDSTEGDPAKYAEWERLTREGYLNGFERVYN
                     GSRAPLFIGNHFETWNGGIYMQAIEDVMRSVCKRQGVRCVSFKQLADWLDAQDPKVLDK
                     LRMLDPAESPDWKSYTK"
     misc_feature    complement(10202..10217)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.9"
                     /tool="antismash"
     misc_feature    11217..11230
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.88"
                     /tool="antismash"
ORIGIN
        1 ggggcacacc ggccatgccg tcgtcatcgg tggcgggatc tcgggcctcg cggccgccca
       61 ccggctccag gcgggcgggg cgcgggtgac ggtgctggag gcgtcggggc ggctgggcgg
      121 caagctgtgc gcgggcgaga tcgagggcgt cgccgtggac ctgggcgccg aatcgatgct
      181 cgcccggcgc ccggaggcgg tggagctggc gcgcgccgtc ggcctcggcg agcggctcca
      241 gccgcccgcg accgcgaccg cctccctgtg gacgcgcggc acgctgcggc cgatgcccaa
      301 gggccatgtg atgggcgtgc ccggcgatcc ggcgccgctc gccgcgtccg gggtgatctc
      361 ggacgagggg ctggcgcgca tcgcccgtga cgcggagctg ccgcccacac cggtcggcga
      421 ggacgtggcg ctcggcgagt tcatcgcggc ccggctcggc cgggaggtcg tggaccggct
      481 ggtcgagccg ctgctcggcg gggtgtatgc gggggatgtg taccgcacct cgatgcgggc
      541 cgcggtgccc accctcttcg aactggcccg cgcgaaccgc ccgctggccg agggggtccg
      601 ggagctgacc gagcgggccg cccagcggca gggcggggcc gggccggtgt tcatgggcat
      661 cgacggcggc atcgggcggc tgccgctcgc ggtggccgac gccgtacggg catcgggcgg
      721 cgacatccgc accggcgcgc cggtacgaga actgcggcgg accgccgacg gctggaccgt
      781 cgtcaccgac gacgaggtgc tgcgggccga cgcggtcgtg gtggcggccc ccgcgcccgc
      841 cgccgcccgg ctgctggcgg ccgactcccc ggccgcctcc gccgagctgg ccaccgtcga
      901 ctacgcctcc atggcgctga tcaccatggc attccgccgc gacgacgtgg tgtcgctcgc
      961 cgacggcagc ggcttcctgg tcccgccggt cgacggccac acgatcaagg cggccacgtt
     1021 ctccagccgc aagtggggct ggctccggga cgccgccccc gatctcttcg tgctgcgtac
     1081 ctcgatcggg cggttcgagg acgaggagca cctgaagcgc gacgacgccg atctggtagg
     1141 gatgtcgctc gccgatctcg gcgcggccac cggcctcgac gcccggccgg tggcgacccg
     1201 ggtgagccgc tgggacggcg ggctgccgca gtacccggtc ggccatctcg accgggtcgc
     1261 ccggatccgc gcggaggtcg ccaagctgcc cgggctgcgg gtgtgcggcg cggtctacga
     1321 cggcgtcggc atccccgcgt gcatcggatc cgcccgcacg gccgccgatg agctgctgac
     1381 ggcgaccctg gcggcgggcg ccgtcggcgg agagcgagaa taggcaccat gactgctgct
     1441 cccgagaccc ccgacacgcc ccagagcgtc ccgtcgccca aggcgcccaa cgcggggaag
     1501 aaggccaagg acctcaacga ggtcatccgc tacaccctgt ggtcggtgtt ccggctgcgc
     1561 gacgtgctgc cggaggaccg caccggctac gccgacgagg tcgagcagct cttcgcacag
     1621 ctcgccgcca aggacgtcac cgtgcgcggc acctatgacg tctcggggct gcgggcggac
     1681 gcggacgtca tgatctggtg gcactcggag acctccgacg cgcttcagga ggcgtacaac
     1741 ctcttccgcc gcacccggct cggccgcgcc ctggagccgg tgtggtcgaa catggcgctg
     1801 caccgcccgg cggagttcaa caagtcgcac atccccgcgt tcctcgcgga cgagaacgcg
     1861 cgcgactacg tcagcgtcta ccccttcgtg cggtcctacg agtggtacct gctgcccgac
     1921 gaggagcgcc gcacgatgct cgcggaccac ggcaagatgg cgcggggctt ccccgatgtg
     1981 cgcgccaaca ccgtcgcgtc gttctccctc ggcgactacg agtggatcct ggccttcgag
     2041 gcggacgagc tgcaccggat cgtcgacctg atgcgccatc tgcgcgcctc cgaggcgcgc
     2101 cgtcatgtgc gcgaggaggt gccgttctac accgggcggc gcaagccggt ctccgagctg
     2161 gtcgccggtc tggcctgacc gcaacgagcc cgaccgtcac ggcatcccgg gcgccccgga
     2221 ccctcccggg ccgccgggga gcgcgggctg cttgccggac tcgggcaccg gggccgggcg
     2281 cggctccggt gccgggcgcg gtgcgcagga ggccgtccgg cccggcgtcc tgccggtcag
     2341 cagatacgtg tcgaccttgc ggttgacgca ggcgttcggg ccgccccaca ggccgtgcgt
     2401 accggccttc cgctcggtca ccagggaggc gccggggagt ctgcgccaca gcgccttggc
     2461 gcccgcgtac ggagtggccg cgtcccgttc ggccgccagc agcagcaccg gcggcagccc
     2521 cggccgggcg gtcacgtcca tgggggtgtc gtgccccagc ggccagtagg cgcagggcag
     2581 gttcatcgcc acgttgtccc aggtctcgaa gggcgcccgg cgcgcgaggg cggtgttctc
     2641 ccggttccag ctcgcccagc ggcgcggcca gggcgcgtcg ttgcactcca cggcggtgta
     2701 gacggcgttg gcgttctcgt cggccaccgc gtccccggat tcggacgcgg ccagcgcgat
     2761 cagcggccgg ggattgccgc gcagataggc ggcgagggcc cgggcgctgg ggtcccagta
     2821 ggcgtcgttg tagcccgccc gcacgaacgc gtcctggagc tccccggtgc cgacgcggcc
     2881 gcccgccggt ttccgcgcca ggcggtgggc cgccttccgg tacgcggcga ggacccgggc
     2941 gggggtcgcg ccgaggtggt agacggcgtg gtgccgggcg gcccagcggc gccagtcggc
     3001 ccagcgccgc tcgaaggcca gggactggtc caggttgttg cggtaccaga tctgccaggg
     3061 gccggggttg accacgctgt cgaacaccat gcggcggaca tggcggggga agagcgtcgc
     3121 gtacacggcg ccgtagtacg tgccgtacga ggcgcccacg aaggtgagcc gcttctcgcc
     3181 gagcgcggcg cgcaggacat cgaggtcgcg ggcgttgttc agcgtggtga tgtgccgcag
     3241 ggccgccccc gtacggcgca cacagccccg ggcgtacgcg cgcgcccgcg ccaccatcgc
     3301 ccgcagatac gccgcggagg ggtgttgcgg ggagctggtc ggcgtctttg cgaaggccgc
     3361 cgggcgctgg caggacaccg gtgcggagcg gccgactccg cggggcgcgt agccgacgaa
     3421 gtcgtaggcc gcggccgtct tgcggaagcc gtcctcggcg gcgtactcgg ggaacgtcat
     3481 gccggacgcg cccggcccgc ccgggttgta gaccagcgcg ccctgccgcc gggccttggg
     3541 cccggaggcg cggacgcggc tgacggtgag acggatgtgc ttgccgtgcg gccgcgcgta
     3601 gttcagcggc acggagacgg tgccgcagcg aacgtccgac gacagctctt cggagctcgg
     3661 gcagcggccg aaggcgatgc cccgggcggc cgcctcccgg gccgccacga tcaccccgcg
     3721 ggcctcggcg gcgccgcccc ggggcacggc cagcgcgggg gaggccagca cggcggaggc
     3781 gggggagccg aacgaggcag tccaggcggg cgccgccgcc gcgacggcgg tcggcgccgg
     3841 gggcccggcg gcggccacgg cggtggggtg tgtgggcggc ggcgcggcga gcggtctgcc
     3901 ggtggcgggg gtggcgagca gcgccgtcat ggccagggac agggacagcg gcccgaggat
     3961 gccgtacagc gctgctgctc tcacaagcca tccctccgca catcacgcaa caaaggggat
     4021 acttggtggg gtgattggcc atgtaaagca ccgggccgcg gtgtcggggc cattggaccc
     4081 caaggtgtcg ggtcaatgac cccaatgcgc cctcagcgcc gcgcgcagcg ccggatcggg
     4141 cagggcggcc gtgccgtgga ccgcgagggc ggtgagcagc tcgccgtcgt cgggctcctc
     4201 gtcggccccg cccccggtgc gctgggcgac gggccgccgg ggcggcacat cgatccggct
     4261 cagcagctgc tgcccggcgg gggaggccca gcgcgagtac ggatgcacct cgacccgcgc
     4321 gatcaccagg cagctggcgg tcaggagcag cggaagcgcg aaccacgcca gtgccggtcc
     4381 cgggcccgac gcgggcggca ccatcagcag cgcgatcacg cccgtcagca ggaccacgcc
     4441 gcacgccacc cgcacctgcc gtaccgcggc cgccacgttc gcgcgggccc ccgcgggcac
     4501 cgcgagcccc gccgccacca gccggtcggc cagcgcccgc accgggtcgg cggtggccag
     4561 cgcggtgcgg accggcggca cgggggactg gccccgcggg cctatcgcgg cgatgaccga
     4621 gcgttcgaga tcgtcccggc cgtccgggtc cacgacggtg acccagccgg tgtgggcgag
     4681 cagcagtcgg cgctcacggg acatcgacac cagggcgaga tcgaccaccc gccgcggccc
     4741 gcccgccagg aacgccgcct cgtacaggct caggggccgc ccgtccgatc gcggaccggc
     4801 ggtggcttcc atcggttggg cggccgcgac cgcggcgagg cacaggcggg tgcagctgac
     4861 caccgccgag ccccacgcga cgagcaggaa caggacccac aacatggccg agttctactg
     4921 gacccgaccg cttccacgcc agagatttcg cgaaatgcgc gattcaggtg ccgaatgctg
     4981 atcgccgcgt acgcccgcgc tcaccctccg ctcagcacga gccgggcccg gcgggcgaag
     5041 cgcgccagcg ggtgcgagga cagcggggcg ggcccggagc gctcctgcca ccacagcgtc
     5101 agccgacgcc gcgtcgccgc gtcccggacg tcaccggccg ccagcagatg ctcgacgaac
     5161 gccatcgcgt cgcggcggta gccgccggtc atcgggcggc cgtcggcgta cccctcgaac
     5221 gcccgccggt agccgtcgcc caggatctcc ggcagctcgg gggcgacctt ggcgaccacc
     5281 ccggcccgct tggacgtcag cgcccggctc tggaccccca gccgctgccg gtcgaagccc
     5341 tccggcgccg gggcgcccgc gacgagcgcc gacagcagcg cggtctgggc gcgcgccagc
     5401 cgctcccgcg cgccatcgat gtccggtatg tcgtttccgt tcgggacgtg cttcgacgtg
     5461 gtcccggcat cggcggccgt caccccggcc gccgtcaccg cgcggatcgc gtccagctcg
     5521 cccgccagct cctcggccgc cgggaagtcc tcgtcccgct ccagcagcac cccgggcggc
     5581 gtcgtacggg cgcacagctc ggcgagcacg tcgagcaccg gctgcgtcac cgggtgggcg
     5641 tggctgtcgt gccacacccc gtcccgttcg acgccgcccg ccacatggac gtacgcgatg
     5701 gcctcggtcg gcagctcgtc cagcgccttg gccgggtcct cgccccggtt gacgtggttg
     5761 gtgtgcaggt tggcgacgtc gatgagcagc cgcacgccgg tccgctcgac cagttcggcc
     5821 aggaactgcc cctcggtcat ctcctcgccg ggccaggaga tcagtgccgc gatgttctcc
     5881 agcgcgagcg gcaccggcag cgcctcctgg gccatcgcca cgttctcgca cagcacgtcc
     5941 agcgcgtccc gggtgcgcgg caccggcagc agatgcccgg cctcgatggc gggcgacccg
     6001 gccagtggcc ccccggcccg tacgaacgcg atgtgctcgg tgaccagcgg cgagcccagc
     6061 gcctccgccc gctccgcgag ggcgctcagc cgctcgggat ccggccgctc ggcgccgccg
     6121 aggccgagcg agacgccgtg cgggaccacc gtcgtaccgc gctcgcgcag ccgcagcagc
     6181 gcgtccggca catggccggg gcagacgttc tcggccacca cctccaccca gtcgatgccg
     6241 ggcaggcgcg cgatgtcctc ggcgatttcc ggtcgccagc cgattccggt accgaggcgc
     6301 gtcatggctc ccccttctcc tgccggtccc atgccggacg ttctcacctg tcggggtaat
     6361 gacccccggg ggagaggacg aatccacggc gcttgtggtt cagaggttca tttgagcttc
     6421 ggggtgtcac gctacgcccc tccgtgcgcc cccggtcgcc cgccttcacg gtctccgacg
     6481 gccgtggcag ggcggtgagt tgcggaatat ctgcactgtt acaaaatttt ggggccctgt
     6541 ggcgacagat gttcggaagg gagaataagg tcgtgtggtc tggggggatg gaagatcaca
     6601 cctttgtgcc gcgcccgcgc gcggagcgag cgtgggggaa cacacgtgct gggcagcaac
     6661 aatgccatgg ggaagcaccg ccgtaacgca caccgtaagc cgccgccgtc gtcggccggg
     6721 cgcaggttgc agctgcgtcg ctgggcggcc cgccatatcc ggatcgacta cccccgggcc
     6781 gggaggcggg gcgcgcggcg gtggctgccg tcgtggcgtc agatgggtct cctcttcgtg
     6841 ttctgcctcg ggagtctgac cggtgtcctg agctttctct acatgcagac cgagataccc
     6901 aagaacctca acgacttcgc cacccagcag gacaacgtct actactgggc cgacggctcc
     6961 gagatggccc gcacgggccc ggtcaaccgt caggaggtgg cgttgtccaa ggtgccggaa
     7021 aaggtccagt gggcgatcct cgccgccgag aacgccagtt tctactcgga cagcggcatc
     7081 tccccgagcg gtctgatgcg cgccgtgacc cggatggtga cgggcggtga gacccagggt
     7141 ggctcgacca tcacccagca gtatgtgaag aacgcctatc tgaatcagcg ccagacgttc
     7201 tcccgcaagc tcaccgagat gttcatggcc atcaagctcg acgaccggat gagcaagcag
     7261 gagattctcg aacgctatct gaacaccagc tggttcgggc gcggcaccta cggcattcag
     7321 cgggcctcct acgcctacta cggcaaggac gtctcccggc tcaacgcgag cgagggcgcg
     7381 atgctcgcct cactgctcaa gggcgccgga actttcgacc cgacgctcag cgccaagaac
     7441 cacacacggg cggtcgagcg ctggaaatgg gtgctggacc ggatggtcga catcggaaag
     7501 ctctccaagg ccgagcgggc gaagtacacc accttccccg acccccatcc ggcaccccgt
     7561 ccggccgggc tcaccggaca ggtcggctat ctggtggaga ccgcgaaggc gtatgtgggt
     7621 tcccacaccg acatctccga cgccgacttc gacctgggtg gctatcagat ccacaccacc
     7681 ttcgagaagt cgaaggtcaa ggcgctcgcc gcctcggtga agaagacccg cgacggcctc
     7741 gaccccaaga accgccggga ggaccggtac gtacgcatcg gcgccgcctc cgtgacgccg
     7801 ggcgggcgca tcgaggcgct gtacggcggc cccggctatc tggaacaggg cttcaacgac
     7861 gcgaacgcct ccgtcgtccc ggccggtacc gccttcacac ccttcgtcta cgcggcggcg
     7921 ctgcgcgacg gcgtgctcaa ggagcggaac agcccccgga cgcccgtcgc ccccaccacg
     7981 ctctacgacg gcaacaacaa ggtcgccgtg aaaacccccg aggggcccta ctgggaccgc
     8041 agcggcaaga tcgtgcgggg cgagaacgac ggcgagcagt cctatgggca ggtcagcctg
     8101 cgccaggcga tcgtccagtc catcaacacc ccgatgatgc agctcggcat ggacgtcggc
     8161 ctcgaccgcg tccgccaggc ctccatcgac gcggggctgt tgcccagcag cttcggcgcg
     8221 cgggtgccca tgttctcgct cggcaccgcg acgcccagct ccatccggat ggccagcgcc
     8281 tacgcgacgt tcaccgcgaa cggcacccat gtgccgccgt tctccgtgag cggactgacc
     8341 cgtaacggcg agaagttcgc cctgcgcaag gaccgcgcca agcgggcgtt cagcgcccag
     8401 gtggcggcgc aggtggacga cgcgctgcac ggcgcggtcc agggcggcac cgggcaggcg
     8461 gcggccgtgg tcgggcccga tacggcgggg aagacgggca cggcgcagga cggcacgtcg
     8521 tcctggttcg tgggctacac cgatgaggtg tccacggccg tgtcgctgtc gcgggtggac
     8581 cccaagaccc aggaactgct gccgctgaag ggaatgggcg gagacgccac cggaaaggcg
     8641 agcgacacct atcccctgga catctggacg cgctatatgg cgggcgcggg caagaagtag
     8701 ccgggagaca gataaaagcc cggcccggtg cgttccgttc gacgcaccgg gccgggcttt
     8761 tgcttcccgc cgtatgacta cttggtgtac gacttccagt ccggcgattc cgccgggtcg
     8821 agcatccgca gcttgtccag gaccttcgga tcctgggcgt ccagccagtc cgcgagctgc
     8881 ttgaacgaaa cgcaccgcac gccctggcgc ttgcacacgc tgcgcatcac gtcttcgatg
     8941 gcctgcatat agatgccgcc gttccaggtt tcgaagtggt tgccgatgaa caggggtgcc
     9001 cggctgccgt tgtagacgcg ttcgaaaccg ttcagatagc cctctcgggt cagccgctcc
     9061 cattcggcgt acttcgccgg atcgccctcg gtgctgtcgc cggactggtt gaagaggaaa
     9121 ttgaagtcca tggagaggac ctgcttctcg ctctccgggt acggcaccag ctggagcggg
     9181 aagttccaga tgccgtcgat cttggacggc cagatctgga agtcgcccgg agaactcgcg
     9241 tcgtagcgcc agcccatcga cttgatcgcc gggatcagat tcttctgtcc ttcgaggcag
     9301 ggggcgcggc cgccgaccag ttcctcgtcg tagtcgaagg gcagcggttc gatgtcggtg
     9361 aaaccggtat tggtcttcca gttcttcacg aaggagtacg cctgctcggt ctcactcttc
     9421 cactgccggg tggtccactc gccgccgccc ttggagccgc agaaatgccc gttgaagtgc
     9481 gagccgatct cattgccctc cagccacgcc ccgcgcagct gctccagcgt ctcgcgtatg
     9541 tgctcatccg tggggaagga tatggcggcc gcgccctgct tgtgcatcgg cggccgatag
     9601 aggctgctct tgtacttggg cagcagatac atgccgctca ggaagaacgt catctgggcg
     9661 tcgctctcct tggcgacctg gcggaagcgg gagaagaggt gatcgccgtt ctccagcgcg
     9721 ccgtcccagg agaagaccac gaactgtggg ggcttctgcc ccggcttcag cttctcgatc
     9781 ttcggctgct tcggctgggg gccggtgtag gacgtcgagc cgtccccgat caccttcacc
     9841 ttgccgtccc agtcggcgta cttgttcttc ttcttggtgc ctctgggggc cttgtccgct
     9901 cgcggcacgg gggcggcggc ctggacgctg ccgtccaccg cgccgctcgt atcccccggc
     9961 gaggtcaggg cgatgagcag cgccacgacc acggccacgg ccgtgagcgc gcccaggggc
    10021 gcggccatgc cataggcgtt ccgcgccgat aatgccttca acgccttcaa ggactgtgat
    10081 gccttcaagg tcttcaacgc cttcaacggg tcccgccccc tggcaacggt tcagttgtgt
    10141 gccccgctca gtatcacaga agtatcacct gctcttcaca tcggctactc accgacggac
    10201 actcaccaac cggtcagcag cagatggttc accagcagcg cgagacccgc ctgcaccgcc
    10261 agccagcggc gcaggtcacg ggcgggaagg agggcggcgg ccggaagcag ccagagcacg
    10321 aacggcagcc agatgcgctc cgtttcggcc ttgctcatcc cggacagatc ggcggtggcc
    10381 agcgcgagca atgcggcgag cacgagcacc acgagcccat ccgtcccgac ctcaccgccc
    10441 agacccccgc ggcgcagccg ccgtaccgcc cccggcgccg cccccaccgc ccggcgggcc
    10501 ccggcgacgg aggcgagacc ggccgcgatc gccgcgttgg ccaggttggc ccacacccaa
    10561 tagccgtacg ggcgatccgc cgcgacccct tggtagtagc gctcgaccag caggtcgtac
    10621 ccctcccacc aggcgaaacc ggccagggtg aacgcgcctg ccaccaccga cgtcccggcc
    10681 agcgcgtacg gcagcggccg caccgtacgg gcgaggagca gcacggccgc ggcgggcagc
    10741 gccatgaggg tgagcccgta cgacacatac gcggtgaggc cgagcagcag cccggcggcc
    10801 agcgcgacgg cgcccggcgc ccgggtcgca cgggtcgccg ccagcgcgag cagcgccagc
    10861 ccccaggccg cgaccgccgc gaaatagccg tccgccgacg cccccaccca caccgcggcc
    10921 ggggcaagca cgaggaaggg cgcggcggtg cgcgccgcgc gctcctgccc cagcgcgcgc
    10981 agcgcgacca ggacggcggc caccgccgag cttccggcgg tgatgcagaa cgcccccgcc
    11041 catccgccgc cgccgagccc gaggcggtcc agcccgacga aggtgagcac ggcgcccggc
    11101 ggatgtccgg cgatgtgcgc gggccagtgg tcgggctggg tgatcaggat gtgcccggtg
    11161 aagccgcgga gcgcggcgcc gacgtcctgg aagcggtcga ggccgcgcag atactcatga
    11221 cgctgcgtca gtcgcccggc cacgccccgc ccccagccgt cgacgagcgc cagcgcccag
    11281 gtccaggcca tcgcggtcgc ccagc
//
