LOCUS       NZ_CP058693            10405 bp    DNA     linear   CON 08-SEP-2024
DEFINITION  Streptomyces sp. Rer75 chromosome.
ACCESSION   NZ_CP058693
VERSION     NZ_CP058693.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Rer75
  ORGANISM  Streptomyces sp. Rer75
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Genome analysis of Streptomyces sp. Rer75
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) College of Pharmacy, Henan University of
            Chinese Medicine, Jinshui East Road No. 156, Zhengzhou, Henan
            450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. Canu V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.63x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013414305.1-RS_2024_09_08
            Annotation Date                   :: 09/08/2024 05:25:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,857
            CDSs (total)                      :: 8,765
            Genes (coding)                    :: 8,460
            CDSs (with protein)               :: 8,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 145 of 305
            Pseudo Genes (incomplete)         :: 204 of 305
            Pseudo Genes (internal stop)      :: 20 of 305
            Pseudo Genes (multiple problems)  :: 62 of 305
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:27:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2538445
            Orig. end                         :: 2548850
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="14"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            1174..1884
                     /locus_tag="HYQ63_RS10465"
                     /old_locus_tag="HYQ63_10465"
     CDS             1174..1884
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180703.1"
                     /locus_tag="HYQ63_RS10465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10465"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_257231784.1"
                     /transl_table=11
                     /translation="MSAPTDGPAAAATTPTSGLQAADSPADGLPAADAQAEFREFFERH
                     HAELARLAHLLLGSSDGADDLAADALVAVWHRWDRVRVAEHPAAYARGVVANLARSRIR
                     SLVRERRRVALFSSERSDRVDDPDVSAMVDVQAALVRLPFRKRACVVLRHAFDLSERDT
                     ARTLGISVGTVKSQTSRGIAELERLLGPPDDETERPAAGTQAQAPPSAPSAQETVRGLR
                     PGRVGHAGHPGGNG"
     misc_feature    1228..1230
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1258..1260
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1884..2663
                     /locus_tag="HYQ63_RS10470"
                     /old_locus_tag="HYQ63_10470"
     CDS             1884..2663
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180702.1"
                     /locus_tag="HYQ63_RS10470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10470"
                     /product="hypothetical protein"
                     /protein_id="WP_179933574.1"
                     /transl_table=11
                     /translation="MIDHRDDRRDVAAELREAAEAHQPDRARMLARVTAGMAAGERRSV
                     RRVPARQWTRVVGPAAGLAAAVAAAGIAVVTTDTADGPQTVRTSSEPAGPPAGEGGDTA
                     RRPGGGTGDGAGRHTPSAFPTSVGPNHLADPAVRSRGAINPNSNPYWAQSDITLTNSEP
                     LTSLTVELRVAENGGVHTTGSWSTLPADDLAVSVRSEDGVLVYRWTLRKGATVPAGRHV
                     FAGQYNHAEGSRDAGRDRYSASGNGPSGAFAVRGDFR"
     misc_feature    complement(2872..2887)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2882..2897
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3055..3603
                     /gene="ectA"
                     /locus_tag="HYQ63_RS10475"
                     /old_locus_tag="HYQ63_10475"
     CDS             3055..3603
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718915.1"
                     /locus_tag="HYQ63_RS10475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10475"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_179933575.1"
                     /transl_table=11
                     /translation="MTAAQADRVGARSEFDLPEGLTLDTPRVEDGAAIWRIARDSKTLD
                     LNSSYSYLLWCRDFAATSVVARDAEGGPVGFITGYIRPDRPETLVVWQVAVDQAWRGRG
                     LAATLLDGLTARVAATGIRGIETTITPDNTASNRLFTSFAERHGAPVEHEVLFDGGLFP
                     DGGHEPEVLYRIGPLADRG"
     gene            3735..4960
                     /gene="ectB"
                     /locus_tag="HYQ63_RS10480"
                     /old_locus_tag="HYQ63_10480"
                     /pseudo=""
     CDS             3735..4960
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-162). E-value: 4.7e-28.
                     Score: 90.1. Matches aSDomain:
                     nrpspksdomains_HYQ63_RS10480_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="HYQ63_RS10480"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10480"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /pseudo=""
                     /sec_met_domain="Aminotran_3 (E-value: 7.9e-23, bitscore:
                     79.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRGWPAVFDRAQGSHMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNNILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHAPVSAGPRHGCSRTRVPASTSPPP"
     aSDomain        3819..4220
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HYQ63_RS10480_Aminotran_3.1"
                     /evalue="4.70E-28"
                     /label="HYQ63_RS10480_Aminotran_3.1"
                     /locus_tag="HYQ63_RS10480"
                     /protein_end="162"
                     /protein_start="28"
                     /score="90.1"
                     /tool="antismash"
                     /translation="FDRAQGSHMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNNILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAG"
     gene            5001..5405
                     /locus_tag="HYQ63_RS10485"
                     /old_locus_tag="HYQ63_10485"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718913.1"
                     /locus_tag="HYQ63_RS10485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10485"
                     /product="ectoine synthase"
                     /protein_id="WP_179933576.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.9e-52, bitscore:
                     175.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTERHVKAKSGTWESKRIVLARERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDENGEKHIITPGTMYLLDGHEKHTMRIKKDFR
                     CVCVFNPPITGREDHDENGVYPLITETEEA"
     gene            5410..6297
                     /gene="thpD"
                     /locus_tag="HYQ63_RS10490"
                     /old_locus_tag="HYQ63_10490"
     CDS             5410..6297
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718912.1"
                     /locus_tag="HYQ63_RS10490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10490"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_179933577.1"
                     /sec_met_domain="PhyH (E-value: 7.5e-37, bitscore: 126.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTDLYPTRGPIEVATPRVDPVVWSEPGAEGPLQASELSDFDR
                     DGFLAIDQLITPDEVAVYRAELDRLIVDPDVRADERSIIEPKSQDVRSVFEVHRISEVF
                     ANLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSLSLALTENYDTNGGLMIMPGSHRTFLGCAGQTPKDNYKKSLQMQDAGIPSDEALSGF
                     ADKHGIKLFTGKPGSATWFDCNCMHGSGDNITPYSRSNVFIVFNSVENTAVEPFAAPVR
                     RPEFIGARDFTPVR"
     gene            complement(6374..7426)
                     /locus_tag="HYQ63_RS10495"
                     /old_locus_tag="HYQ63_10495"
     CDS             complement(6374..7426)
                     /NRPS_PKS="Domain: Aminotran_5 (13-342). E-value: 3.2e-26.
                     Score: 84.0. Matches aSDomain:
                     nrpspksdomains_HYQ63_RS10495_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718911.1"
                     /locus_tag="HYQ63_RS10495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10495"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_179933578.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.7e-20, bitscore:
                     70.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAGAEFAPQTTYLNTASSGLIPARSVAAMKAALDDAAAGGPY
                     ADVAFEAVEETRAAYARLVGVPARRVAAGASVAVYAGLIATSLPSGAEVLTAEGDFSSL
                     VNPFAVRRDLTLRIAPLEELAEAVRPDTALVAVSAVQSADGRIADLDAIRDAARAHGAR
                     TLVDFSQAAGWLPVSAADADFTVSIGFKWLLCPRGTAFLTVPEDLGGLTPVFAGWVAGE
                     KPWDSCYGPVAELARSARRYDESPSLFSYVAARHSLALLHELGTEAVRAHDRALADRFR
                     AGITALGHSCLPAPGSAIVSVPGLGEAAPRLAKADVQVSARAGNLRAAFHLYNTPADVD
                     RLLEALGDGA"
     aSDomain        complement(6401..7387)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HYQ63_RS10495_Aminotran_5.1"
                     /evalue="3.20E-26"
                     /label="HYQ63_RS10495_Aminotran_5.1"
                     /locus_tag="HYQ63_RS10495"
                     /protein_end="342"
                     /protein_start="13"
                     /score="84.0"
                     /tool="antismash"
                     /translation="TYLNTASSGLIPARSVAAMKAALDDAAAGGPYADVAFEAVEETRA
                     AYARLVGVPARRVAAGASVAVYAGLIATSLPSGAEVLTAEGDFSSLVNPFAVRRDLTLR
                     IAPLEELAEAVRPDTALVAVSAVQSADGRIADLDAIRDAARAHGARTLVDFSQAAGWLP
                     VSAADADFTVSIGFKWLLCPRGTAFLTVPEDLGGLTPVFAGWVAGEKPWDSCYGPVAEL
                     ARSARRYDESPSLFSYVAARHSLALLHELGTEAVRAHDRALADRFRAGITALGHSCLPA
                     PGSAIVSVPGLGEAAPRLAKADVQVSARAGNLRAAFHLYNTPADVDRL"
     misc_feature    complement(7422..7435)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.85"
                     /tool="antismash"
     gene            complement(7623..9158)
                     /locus_tag="HYQ63_RS10500"
                     /old_locus_tag="HYQ63_10500"
     CDS             complement(7623..9158)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718910.1"
                     /locus_tag="HYQ63_RS10500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10500"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_257231785.1"
                     /sec_met_domain="Aldedh (E-value: 9.9e-42, bitscore: 142.0,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPVWSVDPRTGKQREQVAVEATAEEVDKVVRAADAARGALADR
                     TVRAAFLRTAADLLDEARDTLVEAADAETALGPARLTGELARTSYQLRSFATIVDEGAF
                     LDVHVDHADATQTPPWPDLRRYKLPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPVV
                     VKAHPDHPATSEVAASALRRAAERVGLPADVISVVHGFDAGLELIKHPLIAAAGFTGSI
                     RGGRALYDAAAARPVPIPFHGELGSLNPVVVTEAAAAERGEQIGSGLSGSMTLGVGQFC
                     TKPGFVLAPAGAAGDALVKSLTEAVSNTDAGVLLDHRMRDNFVAGVKERAELADVEAPV
                     TPGSGGEHSVSPGFLTVPARRLAEQGPHDVLLEECFGPVTVVARYENDAEISAVLGRLQ
                     GNLTATVQISAAEAEGTEGRAGELIAELTPLAGRVLVNGWPTGVAVAPAQHHGGPYPAT
                     TSTSTSVGGTAVERWLRPVSYQDTPPALLPPELRDDNPLGLPRRVNGIREQQG"
     gene            9290..10060
                     /locus_tag="HYQ63_RS10505"
                     /old_locus_tag="HYQ63_10505"
     CDS             9290..10060
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="HYQ63_RS10505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_10505"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_009718909.1"
                     /transl_table=11
                     /translation="MTAAETGGAQVKSAVRTVELLEFFAGRPGMHSLASVQEAVGYPKS
                     SLYMLLRTLVELGWVETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTT
                     ETIHLARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYTDEQVRKLL
                     PETLSPLTEHTITDREKLIEELHLIREQGYAVDREENTLGLRCFGIAIPYRTPSRDAIS
                     CSVPVARLTGAHEQMIKDALFDARDRLTLATRRL"
ORIGIN
        1 tacaccacga ggtcggcggg ggcgccctcg gtgatccccg gccgtcccag ccaggtccgg
       61 gcgccccagg tggccgccga cagcgcgtcc cgcaccggga tgcccgcctt gaccagctcg
      121 gcgacctcct ggccgaccag cccgtgcgcg agcgaaccgc ccgcgtcggt gccggtgtag
      181 acggggatgc ccgcgtccca ggcggcgcgc accgtgtcgt agcgccgcgc gtgcagccgt
      241 cgcatgtggt cggcccagcg ggggaacttg gcctcgccgc cctctgcgag ccgcgggaag
      301 gtggcgatgt tgacgagggt cgggacgatg gcgacgccgc gctcggcgaa cagcgggatg
      361 gtgtcctcgg tcagcccggt ggcgtgctcg atgcagtcga tcccggcctc gaccagcggg
      421 gcgagcgtct cttcggcgaa gcagtgcgcc gtgacccgcg ccccgagccg gtgggcctcg
      481 gcgatggccg cctcgaccgc cccgcgcggc cagcaggcgg acaggtcgcc gctgtcccgg
      541 tcgatccagt cgccgacgag cttgacccag ccgtcgccgc ggcgcgcctc ggccgccacg
      601 taggcgacca ggtcctccgg ttcgatctcg tgcgcgtagt tgcggatgta gcgcttggtg
      661 cgggcgatgt ggcgaccggc ccggatgatg cgtggcaggt cctcgcggtc gtcgacccag
      721 cgggtgtcgg acggcgatcc ggcgtcgcgc agcagcagcg ccccggcgtc gcgttcggtg
      781 agtgcctgct tctcgctcgt cgcgtcgtcc accgcgccgt gggcgtccag gccgacgtgg
      841 cagtgggcgt cgacgagccc cggcagcacc cagccgtcca gccgccggac atcccgggtc
      901 ccggtcggcc ggtcgaaggt gatctttccg tcgatcaccc acacctcgtc ccggacgcca
      961 ccggcgtccc cgtcctccgg gccgaccagg atccgcccct tgatgtgcag caccgcgctc
     1021 tccctcatgt cacgcactgt acgagacggc ggccgaccgc tgaccggcca attcccggga
     1081 agcggaaggg ctccactcgt acaacacggg gagcgctgct acgctcacgc aacccaccgg
     1141 acgagggaca gcagggacag tgaggaggcc cgcgtgagcg ctcctaccga tggcccggcc
     1201 gccgcggcca ccacaccgac gtctggctta caggcagccg actcaccggc ggatggctta
     1261 cccgcggccg acgcgcaggc ggagttccgg gagttcttcg agcggcacca tgccgagttg
     1321 gcccgcctcg cccacttgct gctgggcagt tccgacggtg cggacgacct ggcggccgac
     1381 gccctggtcg cggtctggca ccggtgggac cgggtgcggg tcgccgagca tccggccgcg
     1441 tacgcccggg gcgtggtcgc caacctggcc cggtcccgga tccgcagtct ggtgcgcgag
     1501 cggcggcggg tggcgctctt ctcgtcggag cgctcggacc gggtggacga tccggatgtg
     1561 tccgcgatgg tggacgtgca ggccgcgctg gtgaggctgc cgttccggaa gcgggcgtgc
     1621 gtggtgctgc ggcacgcgtt cgacctctcg gagcgggaca cggcccgtac gctcggcatc
     1681 tcggtgggca cggtcaagag ccagacctcc cgtggcatcg ccgagctgga gcggctgctc
     1741 ggcccgcccg acgacgaaac cgagcggccc gcggcgggca cgcaggcgca ggccccgccg
     1801 tcggcgccgt ccgcgcagga gacggtacgg gggctccgcc ccggccgtgt gggtcatgcc
     1861 gggcacccag gagggaacgg ttgatgatcg accatcgcga cgaccggcgc gatgtcgccg
     1921 ccgagctgcg cgaggccgcc gaggcgcacc agccggaccg ggcccggatg ctggcccggg
     1981 tcaccgcggg catggcggcg ggcgagcggc ggagcgtccg ccgggtcccc gcccggcagt
     2041 ggacccgggt cgtgggcccg gcggcggggc tggccgcggc ggtcgcggcc gccgggatcg
     2101 cggtggtgac caccgacacc gccgacgggc cgcagacggt acggacgtcg agcgaaccgg
     2161 ccgggccgcc cgcgggcgag ggcggcgaca cggcgcgacg gccgggcggc ggcacggggg
     2221 acggggctgg tcggcacacg ccgagcgcct ttcccacctc ggtcggtcca aaccatctcg
     2281 ccgatccggc ggtgcgttcg cggggggcga tcaacccaaa ttccaacccg tactgggcgc
     2341 agagtgacat cacgctcacc aacagcgagc cgctgacgtc gctgacggtg gagctgcggg
     2401 tcgccgagaa cggcggggtg cacaccacgg gatcctggag caccctgccc gcggacgacc
     2461 tggccgtctc ggtgcgctcc gaggacgggg tgctggtgta ccgctggacg ctccggaagg
     2521 gggccacggt gccggccggg cgccatgtct tcgcggggca gtacaaccat gccgagggca
     2581 gccgggacgc cggacgggac cgctactctg cctccggcaa cgggccctcc ggcgcattcg
     2641 ccgtccgcgg tgactttcgg tgagttcggc ggtgagttcc gtgcaagcgg tcgcgagttc
     2701 cacgcgagaa gcgatgcgcc aagcccggcg gaatgaattc cggaagaccg gaagacaaat
     2761 tcggggcgtt tttcatgtac atcttcccat ctcgtcttcc ccctcttccg atctctaaac
     2821 gcactttttt tagtggccca ggacacggta attcgatgcc atcacgcccc cgttacgcac
     2881 atgtgacaca ctccacacgt cgaccgtttc gacccgctaa ctcccccaca aaatagcgct
     2941 tttcagcgat ctgccgcgcg tacgcgcgca ctcgcgtgtt accacatcga cccccgtcta
     3001 cgtaacccct ctcctcgatg catttttaga tcctgctggg taaattgaat ttgcatgacc
     3061 gccgcacaag cagaccgagt aggcgcccgt agcgaatttg atttgccaga ggggctgacc
     3121 ctcgacacac cacgcgtcga ggacggagcc gcgatctggc gaatcgcccg cgactccaag
     3181 actctggacc tcaactcctc ctacagctac ctgttgtggt gccgcgactt cgccgccacc
     3241 tccgtggtgg cgcgcgacgc cgagggcgga ccggtcggct tcatcaccgg ctacatccgc
     3301 cccgaccggc ccgagaccct ggtggtctgg caggtcgccg tcgatcaggc gtggcgcggt
     3361 cgcggactgg cagccaccct gctggacggg ctgaccgcgc gcgtcgccgc gacgggcatc
     3421 cggggcatcg agaccacgat caccccggac aacaccgcgt cgaaccggct gttcacgtcc
     3481 ttcgcagagc ggcacggcgc gcccgtcgag cacgaggtgc tcttcgacgg cgggctgttc
     3541 cccgacggag ggcacgagcc cgaggtgctc taccgcatcg gcccgctcgc ggaccgcggc
     3601 tgagcccggg gcaacggcac ccgaggtcat ccccgatttc tgcccccgat cccccgtaca
     3661 gcgacccccc gtacaccccg agggcgcacg ccctcacccc ctcacccctc gcactcatct
     3721 ctcaggagaa tgctgtgacc atcaccccgc ccgccctgag cgtcttcgag gccctggagt
     3781 cggaggtgcg cagctactgc cgcggttggc ctgccgtctt cgaccgcgcg cagggcagcc
     3841 acatgtacga cgaggacggc cacacctacc tcgacttctt cgccggagcc gggtcactca
     3901 actacggcca caacaacccg gtactgaaac gggcgctgat cgactacatc gagcgtgacg
     3961 gcgtcacgca cggtctcgac atgtcgacca cggccaagcg cgcgttcctg gagtcgttcc
     4021 agaacaacat cctgcgcccg cgtgacctgc cgtacaaggt catgttcccg ggcccgacgg
     4081 gcaccaacgc ggtcgaggcc gcgctgaagc tcgcccgtaa ggtcaagggg cgcgagtcga
     4141 tcgtctcgtt caccaacgcc ttccacggca tgtcgctcgg ctcgctcgcc gtcaccggca
     4201 acgccttcaa gcgggccggc gccggtatcc cgctggtcca cgcgccggta tccgctggtc
     4261 cacggcacgg ctgctcgagg accagggttc cggcctcaac aagcccgccg ccgtgatcgt
     4321 cgagaccgtg cagggcgagg gcggcatcaa cgtcgcccgc gccgagtggc tgcgcgcgct
     4381 ggccgacctg tgcgaacgcc aggacatgct gctgatcgtc gacgacatcc agatgggctg
     4441 cggccgcacc ggtgccttct tctccttcga ggaggcgggc atcaccccgg acatcgtcac
     4501 ggtgtccaag tcgatcagcg gttacggcct gccgctggcg ctcaccctct tcaagccgga
     4561 gctggacatc tgggagcctg gtgagcacaa cggaacgttc cgtggcaaca accccgcgtt
     4621 cgtgaccgcc gccgcgaccc tggacaccta ctgggccgac ggccagatgg agaagcagac
     4681 cctggcccgc ggtgagcagg tcgagcaggg gctccgcgcc atctgcgagg agcagtccgg
     4741 aatctcctac cgcggccgtg gcctggtctg gggcatggag ttcgaggaca agccgcgcgc
     4801 ctcggccgtc tgcaagcgcg ccttcgagct cggcctgctg gtcgagacct ccggcccgga
     4861 gagcgaggtc gtcaagctgc tgccggcgct gacgatgacg cccgaagagc tggacgaggg
     4921 tctgcggatc ctcgcccggg cggtccgcga gaccgcttga aacatcaagt aacggaacag
     4981 cagaaaggta gcgactcacc gtgatcgtcc gatccttcaa ggacatcgag ggcaccgaaa
     5041 ggcatgtaaa ggccaagtcg ggtacgtggg agagcaagcg catcgtgctc gcgcgggagc
     5101 gggtcggctt ctcgctgcac gagaccacgc tctacgcggg caccgagacc tcgatgtggt
     5161 acgccaacca catcgaggcg gtcctctgcg tcgagggcga ggccgagctc accaacgacg
     5221 agaacggcga gaagcacatc atcacgcccg gcaccatgta cctgctggac ggacacgaga
     5281 agcacacgat gcgcatcaaa aaggactttc ggtgcgtttg tgtcttcaac ccgccgatca
     5341 ccggacggga ggaccatgat gagaacgggg tataccccct gatcaccgag accgaggagg
     5401 catgatccga tgaccacggt cactgacctg tacccgaccc gcggccctat cgaggtggcc
     5461 acgccccgcg tggaccccgt cgtgtggtcc gagcccgggg ccgagggtcc tctgcaagcg
     5521 tccgagctga gcgatttcga tcgcgatggc tttctggcga tcgatcagct catcactccg
     5581 gacgaggtcg cggtctaccg cgccgagctg gaccggctga tcgtcgaccc ggacgtgcgc
     5641 gccgacgagc gctcgatcat cgagccgaag tcgcaggacg tacggtcggt gttcgaggtg
     5701 caccggatca gcgaggtctt cgccaacctg gtgcgcgacc cgcgtgtggt gggtcgcgca
     5761 cggcagatcc tcggttcgga cgtctacgtc caccagagcc ggatcaacgt caagccgggt
     5821 ttcggggcct cgggcttcta ctggcactcg gacttcgaga cctggcacgc cgaggacggg
     5881 ctgccgaaca tgcggaccgt gtcgctctcg ctcgccctga ccgagaacta cgacaccaac
     5941 ggcggcctca tgatcatgcc ggggtcgcac cgcacgttcc tcggctgcgc gggccagaca
     6001 ccgaaggaca actacaagaa gtccctccag atgcaggacg ccgggattcc gtccgacgag
     6061 gcgctcagcg gcttcgccga caagcacggc atcaagctgt tcaccggcaa gcccggttcg
     6121 gcgacctggt tcgactgcaa ctgcatgcac ggctcgggcg acaacatcac gccctactcg
     6181 cgcagcaacg tcttcatcgt gttcaacagc gtggagaaca cggcggtgga gccgttcgcg
     6241 gcgcccgtgc ggcggccgga attcatcggg gcgcgggact tcacgccggt gcggtgaacc
     6301 acaggtcccg ttgaccgtgg gtggccaggg ggagggcggc acacgccctc cccctggcca
     6361 tccgcgctgt ccgctacgca ccgtccccca gtgcttccag caggcggtcc acgtcggcgg
     6421 gcgtgttgta gaggtggaag gcggcgcgca ggttcccggc ccgcgccgag acctgtacgt
     6481 ccgccttggc cagccggggc gcggcctcgc cgagccccgg tacggagacg atcgccgacc
     6541 ccggggcggg caggcaggag tgccccaggg cggtgatccc ggcgcggaag cggtcggcga
     6601 gcgcgcggtc gtgggcgcgg acggcctcgg tgcccagctc atggagcagg gcgagcgagt
     6661 gccgggccgc gacgtacgag aacaggctcg ggctctcgtc gtagcgccgc gccgaacggg
     6721 ccaattcggc caccggcccg tagcagctgt cccacggctt ctcccccgcg acccagcccg
     6781 cgaagaccgg ggtcagaccg cccaggtcct cgggcacggt gaggaaggcg gtgccgcgcg
     6841 gacacagcag ccacttgaag ccgatggaca cggtgaagtc ggcgtccgcc gcgctcaccg
     6901 gcagccatcc ggcggcctga ctgaagtcca ccagggtgcg cgctccgtgt gccctggcgg
     6961 cgtcccggat cgcgtcgagg tcggcgatcc ggccgtccgc cgactggacg gcgctcaccg
     7021 cgacgagcgc ggtgtccggc cgtaccgcct ccgccagctc ctccagcggc gcgatgcgga
     7081 gcgtgaggtc gcggcgtacg gcgaacgggt tgaccaggga gctgaagtcg ccctcggcgg
     7141 tgaggacttc ggcgccggac ggcagcgagg tggcgatcag tccggcgtat acggcgaccg
     7201 aggcgcccgc cgcgacgcgc cgtgccggta cgccgaccag ccgggcgtag gcggcgcggg
     7261 tctcctcgac cgcctcgaag gccacgtcgg cgtacgggcc cccggcggcc gcgtcgtcga
     7321 gggccgcctt catggcggcg acggagcgtg cggggatcag accgctggag gcggtgttga
     7381 gatacgtcgt ctgtggcgcg aactcggcgc ccgccaggct ctccatgtcc atccactatg
     7441 agacgggccc accccggcgt ccatcgcaca tctctgtagg gtccccgcca cggatgctta
     7501 cgcatcccca ctgacctgcg ctgattcccg ctaccggaac gcggcacgcc cggaacgcga
     7561 gacggccgcc gggcgggcgg gtcaggtccg cgcgcccgac ggccgtgccg tcaccggggg
     7621 tgtcagccct gctgttcccg gatgccgttc acccggcggg gcagcccgag cgggttgtcg
     7681 tcgcgcagct ccggcgggag cagcgcgggc ggggtgtcct ggtagctgac cggccgcagc
     7741 cagcgctcga ccgcggtgcc gccgaccgag gtggaggtgg aggtcgtcgc cgggtacggg
     7801 ccgccgtgat gctgggcggg ggcgacggcg acaccggtcg gccagccgtt gaccaggacc
     7861 cggcccgcga gcggggtcag ctcggcgatc agctcaccgg cccggccctc ggtgccctcg
     7921 gcctcggcgg cggagatctg gacggtggcg gtcaggttgc cctggaggcg gccgaggacg
     7981 gcggagatct cggcgtcgtt ctcgtaccgg gcgacgacgg tcaccgggcc gaagcactcc
     8041 tccagcagca catcgtgcgg gccctgctcg gcgaggcgcc gggccgggac ggtgaggaag
     8101 ccggggctga cgctgtgctc gccgccggag ccgggggtca ccggggcctc gacgtccgcc
     8161 agctcggcgc gctccttgac cccggccacg aagttgtccc gcatgcggtg gtcgagcagc
     8221 accccggcgt cggtgttgct gacggcctcg gtgagcgact tgaccagcgc gtccccggcg
     8281 gcgcccgcgg gcgccagcac gaagccgggc ttggtgcaga actggcccac gcccagcgtc
     8341 atcgagccgc tgagcccgga gccgatctgc tcgccgcgct cggcggcggc ggcctcggtg
     8401 accacgaccg ggttgaggct gcccagttcg ccgtggaagg ggatcggcac cggccgggcg
     8461 gccgcggcgt cgtacagggc gcggccgccg cggatcgagc cggtgaagcc cgcggcggcg
     8521 atcagcgggt gcttgatgag ttcgaggccc gcgtcgaagc cgtgcaccac ggagatcacg
     8581 tcggcgggca gcccgacccg ctcggcggcc cggcgcagcg cggaggcggc cacctcggag
     8641 gtcgcggggt ggtcggggtg ggccttgacg acgaccgggc agcccgcggc gagggcgctg
     8701 gcggtgtcgc cgccgggtac ggagaaggcc agcgggaagt tgctggcggc gtagacggcg
     8761 acgacgccga gcggcagctt gtagcggcgc agatcgggcc acggcggggt ctgggtggcg
     8821 tcggcgtggt cgacgtggac gtcgaggaag gcgccctcgt ccacgatcgt cgcgaaggag
     8881 cggagctggt agctggtgcg ggccagctcg ccggtgagcc gggccgggcc gagcgcggtc
     8941 tcggcgtcgg cggcctcgac cagcgtgtca cgggcctcgt cgagcagatc ggcggcggtg
     9001 cgcaggaagg cggcgcgcac cgtacggtcg gccagggcgc cgcgcgcggc gtcggccgcc
     9061 cgcaccacct tgtcgacctc ctccgccgtg gcttcgaccg ctacctgctc gcgctgcttt
     9121 ccggttcggg ggtcgacgct ccacactggt gctgacaccg cggattcctc ctgctgtgtt
     9181 accgctgctt gcgtctcgca catcgactgt tgttcgatat gttgaacgct gtcccggttg
     9241 atgaattcca tcgggactct atggctggtc gaacagaggg gtcaaggcga tgacagctgc
     9301 cgagacgggc ggggcccagg tcaaatcggc ggtccgcacg gtcgaactgc tggagttctt
     9361 cgccggacgg cccgggatgc acagcctggc ctccgtgcag gaggccgtgg gctaccccaa
     9421 gtcgagcctg tacatgctgc tgcgcaccct ggtcgagctg ggctgggtgg agacggacgc
     9481 gaccggcacc cggtacggca tcggggtgcg ggcgctgctc gtcggcacgt cctacatcga
     9541 cggcgacgag gtcgtggccg cggcccggcc caccctggac cggctctcgg acgacaccac
     9601 cgagaccatc cacctcgcgc ggctcgacgg caccaacgtg gtctacctcg ccacccgcca
     9661 gtcgcagcac tacctgcgcc ccttcacccg cgtcggccgc cgcctcccgg cccactccac
     9721 ctcgctgggc aaggcgctgc tggccacgta caccgacgag caggtgcgca agctgctgcc
     9781 ggagacgctg agcccgctca ccgagcacac gatcaccgac cgcgagaagc tgatcgagga
     9841 gctgcacctg atccgcgagc aggggtacgc ggtggaccgc gaggagaaca ccctgggcct
     9901 gcgctgcttc ggcatcgcca tcccctaccg caccccgtcc cgggacgcca tcagctgctc
     9961 ggtgccggtg gcccggctga ccggggccca tgagcagatg atcaaggacg cgctgttcga
    10021 cgcccgcgac cgcctcacgc tcgccacgcg ccgcctctga tccgaagacc gcgggtcgcg
    10081 gggaggacgg cggctctcag ggtcgtgggg aggacgggga ggacggcggc ctccgccgca
    10141 cggcggaggc ggatcgtcgg acagggcgag gtgcggagcc ggaggctccg caaggctggt
    10201 gtacatgaca cagccccacg ccaccaccgc tccccgccgg gcgctgcgcg ccgtcgccat
    10261 cctcgcctgc ctcccctaca tcgccctcaa ggtcgcctgg atcgcgggga gttacgtcgg
    10321 catccccgag ggaagcgatc tgcgccgccc cgaggaacgc cccgtcatgc tcgccttcaa
    10381 cgggctctcc gtcctcatgg acgcc
//
