LOCUS       NZ_CP058693            10861 bp    DNA     linear   CON 08-SEP-2024
DEFINITION  Streptomyces sp. Rer75 chromosome.
ACCESSION   NZ_CP058693
VERSION     NZ_CP058693.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Rer75
  ORGANISM  Streptomyces sp. Rer75
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Genome analysis of Streptomyces sp. Rer75
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10474412)
  AUTHORS   He,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2020) College of Pharmacy, Henan University of
            Chinese Medicine, Jinshui East Road No. 156, Zhengzhou, Henan
            450046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. Canu V1.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 76.63x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013414305.1-RS_2024_09_08
            Annotation Date                   :: 09/08/2024 05:25:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,857
            CDSs (total)                      :: 8,765
            Genes (coding)                    :: 8,460
            CDSs (with protein)               :: 8,460
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 5 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 305
            CDSs (without protein)            :: 305
            Pseudo Genes (ambiguous residues) :: 0 of 305
            Pseudo Genes (frameshifted)       :: 145 of 305
            Pseudo Genes (incomplete)         :: 204 of 305
            Pseudo Genes (internal stop)      :: 20 of 305
            Pseudo Genes (multiple problems)  :: 62 of 305
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:27:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1159542
            Orig. end                         :: 1170403
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10861
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="7"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10861
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10861
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5861](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5861)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    393..407
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 22.13"
                     /tool="antismash"
     gene            713..2227
                     /locus_tag="HYQ63_RS04265"
                     /old_locus_tag="HYQ63_04265"
     CDS             713..2227
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056770.1"
                     /locus_tag="HYQ63_RS04265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04265"
                     /product="NADH-quinone oxidoreductase subunit NuoF family
                     protein"
                     /protein_id="WP_179932583.1"
                     /transl_table=11
                     /translation="MIKTKPRLACVDPPRLLAGLDEAYRLDRVGHLTVHGTMPALRADE
                     LVALAENIDLRGRGGAGFPFAKKVSAVVEAAGRAGGRCNVVVNGSEGEPSCLKDTALLL
                     HTPHLVIDGATLAAEAIGAEEVAIAVHRADVEESLNAAVAERGPSGVPVSVTLTPDRFV
                     AGEGGAVINGISGAPAIPNGRKIRSSENGLGGLPTLLSNTETYAQLAVAARMGALPYRS
                     VGLPTEPGTTLLTVGGKYVMETPTGVPLTYVLELCGLTPGQGVLVGGYHGKWLDPKSIN
                     ATTISRESMKKHGARLGAGAVLPIPEDTCPLGETSRVARWLAAETAGQCGPCFIGLPAL
                     ADAIGQVERGGGQSAIDNVRAYINSVRKRGACSHPDGTAGFVTTALDAFPEEFESHARG
                     AGCGRPTMGVLPLPEESQPLALPPGPTSAPPRGERQQRGPMEKLLVDWSLCQGHGLCAD
                     ILPPEVLTLGIDGYPSSARMDVPRQLRGQAVRAVRRCPALALRIEE"
     misc_feature    2303..2312
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.9"
                     /tool="antismash"
     misc_feature    2318..2333
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.22"
                     /tool="antismash"
     misc_feature    complement(2400..2411)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.06"
                     /tool="antismash"
     gene            2425..3417
                     /locus_tag="HYQ63_RS04270"
                     /old_locus_tag="HYQ63_04270"
     CDS             2425..3417
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056769.1"
                     /locus_tag="HYQ63_RS04270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04270"
                     /product="SCO0930 family lipoprotein"
                     /protein_id="WP_179932584.1"
                     /transl_table=11
                     /translation="MEKRRQIAFAGVSAAMVMLTAACGSNDTSAGSANVQPVGGSSQTA
                     SAGASASAGAGAAAAGAGATGGYGDTGAAAGSGEAGSGSKTGPAKELAVKSDAKLGKFV
                     SDSAGMTLYRFDEDTPKPAKSNCNDACATKWPPVPADDATAGAGIDSKKLGKVKRADGS
                     EQLTLEGWPVYRYAGDTKAGDTKGQGVGGTWNALAPDGKPAGKKAGGGALELMVNNNAD
                     LGKIMVNGEGMTVYRFDKDSAWPMKTACVDAACLDKWKPVKAIDTTKVSGVDASKVTSF
                     TRPDGTKQAAFDCWLLYTFAGDKKPGDTNGQGVKGTWFALDDKGKKVTQ"
     gene            3500..4009
                     /locus_tag="HYQ63_RS04275"
                     /old_locus_tag="HYQ63_04275"
     CDS             3500..4009
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712842.1"
                     /locus_tag="HYQ63_RS04275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04275"
                     /product="hypothetical protein"
                     /protein_id="WP_179932585.1"
                     /transl_table=11
                     /translation="MSRVLRAAALGAALLCAGGLVSGCGGSSHADGKKKEKAHAAKELG
                     KPGKPAALTQIADAIGCKATIVTEADELRQGSCQSSKGDRYTMVTFATAKGQKDWLNTS
                     KDYGGMYLVGARWSVTGQSSGSLKPLRAKLGGAIEQGSVHHGGSKSQGGSGSHDGGMKH
                     DGMKMD"
     gene            complement(4036..4713)
                     /locus_tag="HYQ63_RS04280"
                     /old_locus_tag="HYQ63_04280"
     CDS             complement(4036..4713)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056767.1"
                     /locus_tag="HYQ63_RS04280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04280"
                     /product="HAD family phosphatase"
                     /protein_id="WP_179932586.1"
                     /sec_met_domain="HAD_2 (E-value: 6.2e-23, bitscore: 80.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAETLLAWTPAAIVFDCDGTLMDSERHWEEAREVVLADYGAVCD
                     EEFARRTKGLHYTECGRMMAEFAGLPDLADEMTRQLLSAFRRLVAENPVTMPGAAELVH
                     KAAAFAPLAVASNCPRDVVEDGLSKAGLLRYFDHVLVPGGEVRPKPHPDVYLAAARLCG
                     AAPGDALAVEDSHCGFLSASRAGLRVLGVGPRKPVDERPPVDLWVSTLADPDLLKWADS
                     RAA"
     misc_feature    4863..4883
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     gene            complement(5001..5861)
                     /locus_tag="HYQ63_RS04285"
                     /old_locus_tag="HYQ63_04285"
     CDS             complement(5001..5861)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="HYQ63_RS04285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04285"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_257231596.1"
                     /sec_met_domain="AfsA (E-value: 7.8e-19, bitscore: 66.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAREAVHRVSVAEVLLTDVRTLGDGRFEAAAQWPRSHPTFPHGGD
                     GRHHPLMIAETLRELGIYIPTRYYAVPAGAHFLIRDLSFRLDPEAEPRAPHGATDITCR
                     IAVTDVRTAQPTGRFVSGMRLDVGLWAGGRIFARAGGTARFLDGTAYAETRSAHSGQTA
                     RRRLRAAVRPSPAQLGVPAARDVLVVRDGGVVYLDPADPRHPFFFDHWSDHVPGLVLLE
                     GARQAAALATGGLLIRPLACRMKAIRFTESHPPAVVECVSHGRTCVFRLRQADICTAVG
                     VLQYG"
     misc_feature    complement(5938..5951)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.6"
                     /tool="antismash"
     misc_feature    5939..5952
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.9"
                     /tool="antismash"
     misc_feature    complement(5941..5954)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.73"
                     /tool="antismash"
     misc_feature    complement(5944..5957)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.63"
                     /tool="antismash"
     misc_feature    5950..5959
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.18"
                     /tool="antismash"
     gene            6029..6679
                     /locus_tag="HYQ63_RS04290"
                     /old_locus_tag="HYQ63_04290"
     CDS             6029..6679
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712845.1"
                     /locus_tag="HYQ63_RS04290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04290"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_179932588.1"
                     /transl_table=11
                     /translation="MKDDEHVQERAEQTRRSLLEAAASLFDERGYAGTSISDITARSGH
                     TSGAIYFHYTSKERLALAVVQEHFATWPPLIDRHTGLHVPALEQLVRLSFAVARAFRDD
                     IVVRAGARLWTERSLIAAPFPPPFIGWIDAVGAMLERAHGEGELAAHVDPLPAARSIVC
                     AFFGLHTVSEALDGRRLVEDHLGELWTLLLPSLRAAPGHPGHTAELLAAARGA"
     gene            complement(6692..7489)
                     /locus_tag="HYQ63_RS04295"
                     /old_locus_tag="HYQ63_04295"
     CDS             complement(6692..7489)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712846.1"
                     /locus_tag="HYQ63_RS04295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04295"
                     /product="endonuclease I family protein"
                     /protein_id="WP_179932589.1"
                     /transl_table=11
                     /translation="MTRVTRWKSLALLAGVVTVAVTAQTPATAVPTSTAYDDTYYKDAI
                     GKTGEPLKDALNQIISRDVSKLSYDEVWDALKKTDEDPDNSANVILLYTGRSQSKDSNG
                     GNADDWNREHVWAKSHGDFGTSTGPGTDIHHLRPTDVSVNSTRGNKDFDEGGSEVGEAP
                     GNRTDDDSFEPRDQVKGDVARMILYMSVRYEGGEGFPDLEPNDKVDNGSQPYMGRLSVL
                     KKWNDEDPPDAFEKKRNQVIYDDFQHNRNPFIDHPEWVAEIWK"
     gene            complement(7627..8337)
                     /locus_tag="HYQ63_RS04300"
                     /old_locus_tag="HYQ63_04300"
     CDS             complement(7627..8337)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (13-172). E-value: 1.9e-14.
                     Score: 45.9. Matches aSDomain:
                     nrpspksdomains_HYQ63_RS04300_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056764.1"
                     /locus_tag="HYQ63_RS04300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04300"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_179932590.1"
                     /transl_table=11
                     /translation="MTEPPAEPRHRHAVVTGVSSGIGAAIATRLLDEGWRVTGISRTAP
                     PHGHDRLRWLPADLARPEELPEALTPMDEDGPLDALVHAAGVQRSARLGELDAEDGSLM
                     WRIHVQAATVLADALVDRVRDGGRVVLVGSRTMTGVPGKSQYAATKAALTGLARSWAAE
                     LAPRGITVNVVAPGPTDTPMLRDPGRSRTPPVLPPLGRLVRPEEVAGLTSFLLGPEGGA
                     VTGQTLVMCGGASL"
     aSDomain        complement(7822..8298)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HYQ63_RS04300_PKS_KR.1"
                     /evalue="1.90E-14"
                     /label="HYQ63_RS04300_PKS_KR.1"
                     /locus_tag="HYQ63_RS04300"
                     /protein_end="172"
                     /protein_start="13"
                     /score="45.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVTGVSSGIGAAIATRLLDEGWRVTGISRTAPPHGHDRLRWLPAD
                     LARPEELPEALTPMDEDGPLDALVHAAGVQRSARLGELDAEDGSLMWRIHVQAATVLAD
                     ALVDRVRDGGRVVLVGSRTMTGVPGKSQYAATKAALTGLARSWAAELAPRGITVN"
     CDS_motif       complement(7834..7959)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HYQ63_RS04300_0002"
                     /evalue="4.20E-01"
                     /label="PKSI-KR_m1"
                     /locus_tag="HYQ63_RS04300"
                     /protein_end="168"
                     /protein_start="126"
                     /score="3.4"
                     /tool="antismash"
                     /translation="GRVVLVGSRTMTGVPGKSQYAATKAALTGLARSWAAELAPRG"
     CDS_motif       complement(8236..8298)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HYQ63_RS04300_0001"
                     /evalue="3.20E-02"
                     /label="PKSI-KR_m1"
                     /locus_tag="HYQ63_RS04300"
                     /protein_end="34"
                     /protein_start="13"
                     /score="7.0"
                     /tool="antismash"
                     /translation="VVTGVSSGIGAAIATRLLDEG"
     gene            complement(8330..9580)
                     /locus_tag="HYQ63_RS04305"
                     /old_locus_tag="HYQ63_04305"
     CDS             complement(8330..9580)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056763.1"
                     /locus_tag="HYQ63_RS04305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04305"
                     /product="exo-alpha-sialidase"
                     /protein_id="WP_257231599.1"
                     /transl_table=11
                     /translation="MTYTSTTAHHEEPGAMTADHDTAIPLTDGRLRPRPGDPARQEAFL
                     PAPAVQNHAANLTVLPGGDLGCVWFGGTQEGVPDISVWFSRLAPGSDTWSEPVRLSDDP
                     ARSEQNPLLFPTPAGELWLLYTAQQAGNQDTAEVRVRVSDDNGDTWGAPRTLFPATGGG
                     GVFIRQPIAVLDGGRWLLPVFHCVSTPGAKWVGDHDTSAVMLSDDEGRTWRERPVLGST
                     GCVHMNVHPLPDGTLLALFRSRWADAVYRSHSTDGGESWSEPEPTELPNNNSSVQYVPL
                     GDGRLALVYNHSSRADATARRVSLYDEIDDDGQAGDSPAAAPAQPEDTAGAFWGAPRAP
                     MTLALSADGGRTWPVRRDLDVGDGHCLTNNSRDRLNRELSYPTIRQTPDGTLHIAYTYH
                     RQAIKYVRVSPDWAADD"
     gene            complement(9707..10726)
                     /locus_tag="HYQ63_RS04310"
                     /old_locus_tag="HYQ63_04310"
     CDS             complement(9707..10726)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056762.1"
                     /locus_tag="HYQ63_RS04310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HYQ63_04310"
                     /product="aldo/keto reductase"
                     /protein_id="WP_218929543.1"
                     /transl_table=11
                     /translation="MNAHAQTHTTSQASPGGVTVPGAPVPLSRLVLGTMTFGDTVDRAG
                     AEAMLDAALDAGVTGVDTANAYVGGVTETLLAELLPGRRDRIVLATKAGMPHADAGEHS
                     PLSARGMRAALEGSLRRLGTDHVDLFYLHQPDRATPLEETLSTVAEFVAEGKVRALGVS
                     NYAAWQIAELTHTADRVGAPRPVVAQQLYNLLARRIEEEYREYAATSGLRTMVYNPLGG
                     GLLTGRHAFEETPETGRFGDSKLAAMYRQRYWDPRLFEAVRDLGRIADEASIPLTDLSL
                     RWLLGRDGVDALLLGGSRIEHLRANLAAAAAGPLPEDVVAACDEVGARLRGPMPAYNR"
ORIGIN
        1 acgccggcgc tttcagccag actttcgcgt tcctcgactt cggcgccggg gtgctcgccc
       61 tggtctgcct cagctccgcg gtgctgtggg ggctcgccgc gaccgaccaa ctgatcctgc
      121 actcgaacca ccggctcatc gcccagggca tccaccgggc ggccgcggtc gccggcctcc
      181 tgtttctcgg ccttcacatc tgggtgaagg tcgccgagag ccacacgaac gcagcttcga
      241 tcgtgttacc gttcgctgac gcaaagcagc cggtgctgat cgggctcgga accattgccg
      301 gttacctctt cgtcatcgca gcggtgaccg gagcggtccg cagctcgttc gccgccgcgg
      361 gcaactcccg ctggcggtac cggtggtggc gggcactcca tgtgggcgcc taccctgcct
      421 ggggcctcgc gctggtccat ggtctgaagg ccggacgtga ggccaagatc tgggcgacgt
      481 acggctacat cgcggctctg gccggggtgg ccatcgcgct gtggatgcgg ctcgcagccc
      541 ggcgcagcca ggacgacgat cgaggggacg gcatctcggt gaggaacccg cccgcccgcg
      601 gccttcagga aggccgcacc cggcgcgccg cgcccaccgt gccgcggccc cgctcggccg
      661 actcgggccg gcgggacggt gcatcactcg gtgcctcccg tgggtgggac cggtgatcaa
      721 gacaaaaccc aggctggcct gtgtcgatcc gccccggctg ctggccgggc tggacgaggc
      781 gtaccggctc gaccgggtcg gccacctgac ggtgcacggc acgatgcccg ccctgagggc
      841 ggacgaactc gtcgcgctcg cggagaacat cgatctccgc gggcgcggcg gggccggttt
      901 ccccttcgcc aagaaggtca gcgcggtcgt cgaggcggcc ggccgcgcgg gcggccgttg
      961 caacgtggtg gtcaacggca gtgagggcga gccgagttgt ctcaaggaca ccgcgctgct
     1021 gctgcacacc ccgcacctgg tcatcgacgg tgccaccctg gccgccgagg cgatcggcgc
     1081 cgaggaggtc gccatcgcgg tgcaccgggc ggacgtcgag gagtcactga acgccgccgt
     1141 ggccgaacgc ggccccagcg gcgtcccggt gtcggtcacc ctcacgcccg accggttcgt
     1201 ggccggtgag ggcggtgccg tcatcaacgg catcagcggc gccccggcca tccccaacgg
     1261 ccggaagatc cggtccagcg agaacggtct cggcgggctg cccaccctgc tgtccaacac
     1321 cgagacctat gcccagctcg ccgtcgcggc gcggatgggc gccctgccgt accgctcggt
     1381 ggggctgccc accgagccgg gcacgacact gctgacggtc ggcggcaagt acgtgatgga
     1441 gacgccgacc ggagtgccgc tgacctacgt cctggagctg tgcgggctca cccccggcca
     1501 gggcgtgctg gtgggcggct accacggcaa gtggctggac ccgaagagca tcaacgccac
     1561 caccatctcg cgcgagtcca tgaagaagca cggcgcgcgg ctgggtgcgg gcgcggtgct
     1621 gcccatcccc gaggacacct gcccgctcgg cgagacctcg cgggtggccc gctggctggc
     1681 cgccgagacg gccggtcagt gcggtccctg cttcatcggg ctgcccgcgc tcgcggacgc
     1741 gatcggccag gtggagcggg gtggcgggca gtcggccatc gacaatgtgc gcgcgtacat
     1801 caactcggtg cgcaagcgcg gcgcgtgcag ccaccccgac ggtacggccg gcttcgtgac
     1861 caccgcgctg gacgcgttcc ccgaggagtt cgagtcccat gcacgcggcg cgggctgcgg
     1921 gcggcccacc atgggtgtcc tgccgctgcc cgaggagtcc cagccgctgg ccctgccgcc
     1981 cgggccgacg tccgccccgc cgcgcgggga gcggcagcag cggggcccga tggagaagct
     2041 cctggtggac tggtcgctgt gccagggcca cgggctgtgc gcggacatcc tgccgcccga
     2101 ggtgctgacg ctcggcatcg acggatatcc gtcctccgcc cggatggatg tgccccgtca
     2161 gctgcgcgga caggcggtgc gcgcggtgcg ccggtgcccc gcgctggcgc tgcgcatcga
     2221 ggagtagccc gccttcgaag tccccctccc gcgacggccg ttgcgcaacg gccgtcgctt
     2281 gtttgtgcgc cctcgtatcc agcgtttttt gcgtctggtg acaaattttt aactggctga
     2341 acgtccctcg gcgcgggctc cgtatgggca tggtgtcggc gccttcaccg ccggacaccg
     2401 ataaggattt cgcagggagt ggtcatggag aagcggcgtc agatcgcatt cgcgggagtg
     2461 tccgcggcga tggtcatgct gacggcggcg tgtggcagca atgacacctc cgcgggcagc
     2521 gcgaacgtcc agcccgtggg tggcagcagc cagacggcga gtgcgggtgc ttcggcgtcg
     2581 gcgggtgcgg gcgcggcggc cgcgggggcc ggcgcgaccg gcgggtacgg tgacaccggc
     2641 gcggcggcgg gcagcggcga ggcgggcagc gggagcaaga cgggcccggc gaaggagctc
     2701 gcggtcaaga gcgacgccaa gctcggcaag ttcgtctccg acagcgccgg tatgaccctc
     2761 taccggttcg acgaggacac cccgaagccg gccaagtcca actgcaacga cgcgtgcgcc
     2821 accaagtggc cgccggtgcc cgcggacgac gccacggccg gtgcgggcat cgactccaag
     2881 aagctcggca aggtcaagcg cgccgacggc agcgagcagc tgaccctgga gggctggccg
     2941 gtctaccgct acgcgggcga caccaaggcg ggcgacacca agggccaggg cgtgggcggc
     3001 acctggaacg cgctggcccc ggacgggaag ccggccggca agaaggccgg tggcggcgcc
     3061 ctggagctga tggtcaacaa caacgccgac ctcggcaaga tcatggtgaa cggcgagggc
     3121 atgaccgtct accggttcga caaggacagc gcgtggccga tgaagacggc ctgcgtcgac
     3181 gccgcctgcc tggacaagtg gaagccggtc aaggccatcg acaccaccaa ggtctccggg
     3241 gtggacgcct cgaaggtgac ctccttcacc cgccccgacg gcaccaagca ggccgccttc
     3301 gactgctggc tgctctacac cttcgccggg gacaagaagc ccggcgacac caacggccag
     3361 ggcgtcaagg gcacctggtt cgcgctcgac gacaagggca agaaagtgac ccagtaagac
     3421 ggcatgatgg tccatcccga cgggggcccg gagcggcggc gcttcgggct ccccgccgga
     3481 cgtcgacggg agggttgaag tgtcacgcgt actccgtgcc gcggcgctcg gtgcggcatt
     3541 gctctgtgcg ggcggcctgg tgagcggctg cggcggaagc agccatgcgg acggcaagaa
     3601 gaaggagaag gcgcacgccg ccaaggagct ggggaagccg ggcaagcccg cggctctgac
     3661 gcagatcgcc gacgccatcg gctgcaaggc gaccatcgtc accgaggcgg acgaactgcg
     3721 ccagggctcc tgccagtcca gcaagggcga ccggtacacc atggtcacct tcgccaccgc
     3781 gaagggtcag aaggactggc tgaacacctc gaaggactac gggggcatgt atctggtcgg
     3841 cgcccgctgg tcggtcaccg gtcagtcctc gggatccctc aagcccctgc gcgcgaagct
     3901 cggcggggcc atcgagcagg gctcggtgca ccacggtggg tcgaagtccc agggcggttc
     3961 cgggtcccac gacgggggca tgaagcacga cgggatgaag atggactgac cggtgcccgg
     4021 accctcgctc ggggctcaag ccgcccggga atcggcccac ttgagcagat cggggtcggc
     4081 gagcgtggag acccacagat ccaccggcgg acgttcgtcg acgggtttgc gcggtcccac
     4141 gccgagcacc ctcagcccgg cccgggaggc ggacaggaag ccgcagtggg agtcctccac
     4201 cgcgagcgcg tcaccggggg ccgcaccgca caggcgggcg gccgccagat acacatcggg
     4261 gtggggtttg ggccgcactt ccccgccggg gaccagcaca tggtcgaagt agcgcagcag
     4321 cccggccttg gagagcccgt cctcgaccac gtcgcgcggg cagttgctcg ccacggccag
     4381 cggtgcgaac gccgccgcct tgtggacgag ttcggccgca cccggcatgg tgaccgggtt
     4441 ttccgcgacc agtctgcgga atgcgctcag cagctgccgg gtcatctcgt cggcgagatc
     4501 gggaagtccg gcgaattcgg ccatcatgcg gccgcattcg gtgtagtgga ggcccttggt
     4561 gcgccgcgcg aactcctcgt cgcagacggc cccgtagtcc gcgagcacca cttcgcgagc
     4621 ctcttcccag tgccgttcgg agtccatcag agtgccgtca cagtcgaaga cgatggcggc
     4681 gggggtccat gcgagaagcg tttctgctgt catctgcctg ccgttccagg agagatgggg
     4741 gaatcggcgc agggtgtgcc tgtagccacg gggagcagcg cggtgaattt ctgggcgggc
     4801 gctgcgcgcg gaatgccgga tgcggcatgg gaaagccgta caggcggaga attgcctgcc
     4861 cgaaacataa cgatcactac gttagtgaga tgactccagg taagcaaggt gtcaccgagc
     4921 gtcggcgatt tgttcgtcgc tcagtacgtt cgtagctcgc tgtgttcgtc gctcggggcg
     4981 ttcgtcgtcc tggtgtgcgg tcagccgtac tggagcacac cgaccgccgt gcagatgtcc
     5041 gcctggcgca gccggaagac acacgttctg ccgtgggaga cgcactcgac cacggcgggc
     5101 ggatgggact cggtgaaccg gatcgccttc atccggcagg ccaggggccg gatcagcagg
     5161 cccccggtgg ccagcgccgc ggcctgccgg gcgccctcca gcagcaccag ccccggcaca
     5221 tggtcgctcc agtggtcgaa gaagaacggg tggcgggggt ccgcgggatc caggtacacc
     5281 actcccccgt cccgcaccac cagcacgtcc cgggccgccg gcacgcccaa ctgcgctggt
     5341 gagggccgta cggcggcacg cagtcggcgc ctcgcggtct ggcccgagtg tgcggagcgg
     5401 gtctccgcgt aggccgtgcc gtcgaggaag cgggcggtgc ccccggcccg tgcgaagatc
     5461 cggccacccg cccacaggcc gacgtcgagc cgcatcccgg acacgaaccg cccggtgggc
     5521 tgcgcggtgc ggacgtccgt caccgcgatc cggcaggtga tgtcggtcgc cccgtgcggg
     5581 gcgcggggct cggcctccgg gtcgagccgg aaggacaggt cgcggatcag gaagtgtgcg
     5641 cccgcgggca cggcgtagta ccgggtgggg atgtagatcc ccagttcgcg cagcgtttcg
     5701 gcgatcatca gcggatggtg ccgaccgtcc ccgccgtgcg ggaacgtcgg atgggaccgg
     5761 ggccactggg cggccgcctc gaaccgtccg tcacccagcg tacggacgtc ggtgagcagc
     5821 acctcggcca ccgagacccg gtgcacggcc tcacgtgcca cggtgcggga ccagctgagc
     5881 ggccgaccgg tgggaccggt cggctccgaa aggccgcgag cctcgcgaac gatcaccata
     5941 aaatactaat aatgtttgac ctgtctgtcc tcatcaccat cagcggccga aagtccgcta
     6001 agttcactcc gtcacaccgg accggctgat gaaggacgac gaacacgtgc aagaacgggc
     6061 cgaacaaaca cgccggtctc ttctggaagc ggccgcatcc cttttcgacg aacgcggata
     6121 cgcgggaacg agcatcagcg acatcaccgc ccgttcgggt cacaccagcg gcgccattta
     6181 cttccactac accagcaagg aaagactcgc cctcgccgtg gtgcaggaac acttcgccac
     6241 ctggccgccc ctgatcgacc ggcacaccgg gctccacgtg cccgccctgg agcagctcgt
     6301 ccggctcagc ttcgcggtgg cccgcgcctt ccgcgatgac atcgtggtac gggcgggggc
     6361 ccggctgtgg accgagcgct ccctgatcgc ggcacccttc ccgcccccgt tcatcggctg
     6421 gatcgacgcc gtcggggcca tgctggaacg ggcccacggc gagggcgagc tggccgccca
     6481 cgtcgatccg ctgcccgcgg cccggagcat cgtgtgtgcc ttcttcggcc tgcacacggt
     6541 gtccgaggcc ctcgacgggc ggcggctcgt cgaggaccat ctgggcgaac tgtggaccct
     6601 gctgctgccc tcgctgcggg ccgcccccgg acaccccgga cacaccgccg agctgctggc
     6661 ggcggcccgg ggcgcgtgac cgggggccgg gctacttcca gatctcggcg acccactccg
     6721 ggtggtcgat gaacgggttc cggttgtgct ggaagtcgtc gtagatgacc tggttgcgct
     6781 tcttctcgaa ggcgtccggc gggtcctcgt cgttccactt cttcagcacg gagagccgtc
     6841 ccatataggg ctggctgccg ttgtcgacct tgtcgttggg ctccaggtcg gggaacccct
     6901 cgccaccctc gtagcggacc gacatgtaca ggatcatccg ggccacatcg cccttgacct
     6961 ggtcacgggg ctcgaaggag tcgtcgtcgg tgcggttgcc cggcgcctcc cccacctccg
     7021 agccgccctc gtcgaagtcc ttgttgccac gcgtgctgtt gaccgagacg tcggtcgggc
     7081 gcagatggtg gatgtccgtg ccgggcccgg tggacgtgcc gaagtcgccg tgcgacttcg
     7141 cccagacgtg ttcgcggttc cagtcgtcgg cgtttccgcc gttcgagtcc ttgctctggg
     7201 accggccggt gtagagcagg atgacgttgg cggagttgtc cgggtcctcg tcggtcttct
     7261 tgagggcgtc ccagacctcg tcgtacgaga gcttgctgac gtccctgctg atgatctggt
     7321 tcagggcgtc cttcagcggc tccccggtct tgcctatcgc gtccttgtag tacgtgtcgt
     7381 cgtacgccgt gctggtgggt accgcggtgg ccggggtctg cgccgtgacg gcgaccgtca
     7441 cgacgccggc cagcagtgcc agggatttcc agcgggtcac acgggtcatg tgtggtgtcc
     7501 tctccccgat ttacgaccgt tgttcgatcc ccgaaagcgt gtcagacaca ggatcgtcca
     7561 atggtgaaca tcatgaagag attccgtgtc agggccatga ccgaatcagg accctggacc
     7621 cggccgtcac agcgaggcgc ccccgcacat caccagcgtc tggccggtca ccgcaccgcc
     7681 ctccgggccc agcaggaacg acgtcagccc ggccacctcc tcggggcgta cgagccgacc
     7741 gagcggcgga aggacgggcg gggtacggct gcggcccggg tcccgcagca tcggggtgtc
     7801 cgtcggcccc ggcgccacca cgttcacggt gatcccgcgc ggcgccagtt cggccgccca
     7861 ggaccgggcc aggcccgtca gcgccgcctt ggtggccgcg tactggctct tgcccgggac
     7921 gccggtcatc gtccggctgc ccaccagcac cacccggccg ccgtcccgga cccggtccac
     7981 cagcgcgtcc gccaggacgg tcgccgcctg gacgtggatg cgccacatga gggacccgtc
     8041 ctcggcgtcc agttctccca gccgggcgga gcgctggacg cccgccgcgt gcacgagggc
     8101 gtccagcggg ccgtcctcgt ccatcggggt gagcgcctcc ggcagctcct ccggccgggc
     8161 caggtcggcc gggagccagc gcagccggtc gtgcccgtgc ggcggggccg tacggctgat
     8221 cccggtgacc cgccacccct cgtccagcag ccgggtggcg atggcggcgc cgatgccgga
     8281 gctgacgccg gtgaccacgg cgtgacggtg gcggggctcg gccgggggct cagtcatcgg
     8341 ccgcccagtc cgggctgacg cggacgtact tgatggcctg gcggtggtac gtgtacgcga
     8401 tgtgcagcgt gccgtccggg gtctgccgga tggtgggata cgacagctcc cggttgagcc
     8461 ggtcgcgtga gttgttggtc aggcagtgtc cgtcgcccac gtccagatcg cgccgtacgg
     8521 gccaggtgcg gccgccgtcg gcggacagcg cgagggtcat cggcgcccgg ggcgcgcccc
     8581 agaaggcgcc ggccgtgtct tctggttgcg cgggtgcggc ggccggggaa tcgcccgcct
     8641 gtccgtcgtc gtcgatctcg tcgtacagcg agacccggcg ggccgtcgcg tccgcgcggc
     8701 tgctgtggtt gtacaccaac gccagccgtc cgtcgccgag cgggacgtac tggacggagg
     8761 agttgttgtt gggcagctcg gtgggctccg gctcgctcca gctctccccg ccgtccgtgc
     8821 tgtgcgagcg gtacaccgcg tcggcccagc ggctgcggaa cagggccagc agcgtgccgt
     8881 ccgggagcgg gtggacgttc atgtgcacac agccggtgga gcccagcacg ggccgctccc
     8941 gccaggtgcg gccctcgtcg tcgctgagca tcaccgcgct ggtgtcatgg tcgccgaccc
     9001 acttggcgcc gggggtggac acgcagtgga acaccggcag cagccaccgc ccgccgtcca
     9061 ggaccgcgat cggctgacgg atgaacaccc cgcccccgcc ggtggccggg aagagggtgc
     9121 gcggcgcgcc ccaggtgtcc ccgttgtcgt ccgacacccg gacccggacc tccgcggtgt
     9181 cctggttgcc cgcctgctgg gcggtgtaga gcagccacag ctcaccggcc ggggtgggga
     9241 acagcagcgg gttctgctcc gagcgcgccg gatcgtcgga cagccggacc ggctccgacc
     9301 aggtgtcgga gccgggggcc agccgggaga accacacgga gatgtccggc acgccctcct
     9361 gggtgccgcc gaaccatacg cagccgaggt cgccgccggg cagcacggtc aggttggcgg
     9421 cgtggttctg gacggcgggt gcgggcagga acgcctcctg gcgcgccggg tcgcccggcc
     9481 gcggccggag ccgcccgtcc gtcagcggta tcgcggtgtc atggtcggcg gtcatggcgc
     9541 cgggctcctc gtggtgtgcg gtggtactgg tgtacgtcat cggccggggc tccccgccct
     9601 ggccctcccc cggcacccgg ccccacggga cacgcccggg gcgttcgggg gcccgggggc
     9661 ggggcccccg gttcgggaag gggcggggtg gggaaagtcc acgggcttac ctgttgtacg
     9721 cgggcatcgg gcccctcagc cgggcgccca cctcgtcgca cgccgccacc acgtcctccg
     9781 gcagcggacc cgcggcggcc gccgccaggt tggcgcgcag atgctcgatc cgggagccgc
     9841 ccagcagcag ggcgtccacg ccatcgcggc ccagcagcca gcgcagcgac aggtcggtca
     9901 gcgggatgga cgcctcgtcc gcgatccgcc ccaggtcgcg gaccgcctcg aagaggcggg
     9961 ggtcccagta gcgctgccgg tacatcgcgg cgagcttgga gtcgccgaag cggccggtct
    10021 cgggggtctc ctcgaaggca tgacgccccg tcaataggcc accgcccagc gggttgtaga
    10081 ccatcgtgcg caggccactg gtggccgcgt actcccggta ctcctcctcg atgcgccggg
    10141 ccagcaggtt gtacagctgc tgcgccacca ccgggcgcgg ggcgcccacc cggtcggcgg
    10201 tgtgggtcag ttcggcgatc tgccaggcgg cgtagttgga gacgccgagt gcgcggacct
    10261 tgccctcggc gacgaactcg gcgaccgtgc tcagcgtctc ctccagcggg gtcgcgcggt
    10321 cgggctggtg gaggtagaag aggtcgacgt ggtcggtgcc gaggcggcgc aggctgcctt
    10381 cgagggccgc gcgcatgccg cgcgcggaca acggggagtg ctcaccggcg tcggcgtgcg
    10441 gcatcccggc cttggtggcg aggacgatcc ggtcgcggcg gccgggcagc agctcggcga
    10501 ggagggtttc ggtgacgccc ccgacgtacg cgttggcggt gtccacgccc gtcaccccgg
    10561 cgtcgagcgc ggcgtccagc atcgcctcgg cgcccgcccg gtccaccgtg tcgccgaagg
    10621 tcatggtgcc cagcaccagc cgggacagcg gcaccggggc cccgggcacc gtcaccccgc
    10681 cgggcgacgc ctgggaggtg gtgtgggtct gcgcgtgcgc gttcactgcg ttgctcctga
    10741 tgttcggtga tggtcctggc catggccatg gtcctggtcg gtcacgatcg cggacaccag
    10801 tgcgcgcggt ttgcgccccc gcatcgtcgc cgggtcgagc gccgcgcgcc gcagcgccct
    10861 g
//
