LOCUS       NZ_CP040752            11245 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces rectiverticillatus strain B15-08 chromosome, complete
            genome.
ACCESSION   NZ_CP040752
VERSION     NZ_CP040752.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces rectiverticillatus
  ORGANISM  Streptomyces rectiverticillatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7679243)
  AUTHORS   Park,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2019) Laboratory of Bioorganic Chemistry, NIDDK,
            NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2018
            Assembly Method                   :: HGAP v. Dec-2018
            Assembly Name                     :: DDK_LBC_B15-08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013394065.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:49:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,642
            CDSs (total)                      :: 6,543
            Genes (coding)                    :: 6,394
            CDSs (with protein)               :: 6,394
            Genes (RNA)                       :: 99
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 55 of 149
            Pseudo Genes (incomplete)         :: 125 of 149
            Pseudo Genes (internal stop)      :: 12 of 149
            Pseudo Genes (multiple problems)  :: 41 of 149
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:46:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7250310
            Orig. end                         :: 7261555
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040752.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from LBC, NIDDK.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11245
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="40"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11245
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11245
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6245](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6245)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(505..807)
                     /locus_tag="FGW37_RS31380"
                     /old_locus_tag="FGW37_31660"
     CDS             complement(505..807)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571293.1"
                     /locus_tag="FGW37_RS31380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31660"
                     /product="thiamine-binding protein"
                     /protein_id="WP_179084166.1"
                     /transl_table=11
                     /translation="MLIAFTLTPVGVGESFPDEIADAVRIIRESGLPYRSDSMYTMIEG
                     EWEEVMDVVRRATEAVAARAPRVQLVLKADLWPGKPGMLDGKISMFEQHMADRGQ"
     gene            complement(875..2026)
                     /locus_tag="FGW37_RS31385"
                     /old_locus_tag="FGW37_31665"
     CDS             complement(875..2026)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093225.1"
                     /locus_tag="FGW37_RS31385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31665"
                     /product="MAB_1171c family putative transporter"
                     /protein_id="WP_179084167.1"
                     /transl_table=11
                     /translation="MLTWIENAGIAALWSAAVVRAPQAVRHREQRPLWFAVVMIALAMS
                     MHLEPVTAVLARIVPGAHWVDLTTHLFSIVDAAAVLWFIHGAAGRHRRTHLLFGTAVAV
                     MAALVLLDVLGPAHARNQIAPSRSTASVPDAYWWVFFAFHLTADTFCGFVCWSYGRHGT
                     PRLLRYGLRLFGTGILLASVLWSLKLVYLSTRSPVFAPLFSPVTGVEAAFMALGVALPV
                     FARIRTHLQYRKSYRGLEQLWQDLTTHTPDVVLRPANRIGAAAVPLQLRLYRRVIEIRD
                     AMIVLRNYIPPATLESIRQSVGRQSLPEHLVEAHITASWLTAALRNKHDGHKPHAQTAN
                     LTGPTDRDADESSARSLTHEIDRLLLVASAYHATTTAPQPAGK"
     gene            complement(2011..2376)
                     /locus_tag="FGW37_RS31390"
                     /old_locus_tag="FGW37_31670"
     CDS             complement(2011..2376)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FGW37_RS31390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FGW37_31670"
                     /product="hypothetical protein"
                     /protein_id="WP_179084168.1"
                     /transl_table=11
                     /translation="MLLSVETQDFILYDGQTSPLHQEHIIVHEIGHLVCNHRSALDDTR
                     LHRHLDITDPRSVRRVLPRIRYGDEQEQEAEMIATLILEAAGRVPAPTLLSGMLGGLES
                     AMGLRVGRPAGASCSPG"
     gene            complement(2770..3996)
                     /locus_tag="FGW37_RS31395"
                     /old_locus_tag="FGW37_31675"
     CDS             complement(2770..3996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718776.1"
                     /locus_tag="FGW37_RS31395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31675"
                     /product="aromatic acid exporter family protein"
                     /protein_id="WP_246430661.1"
                     /transl_table=11
                     /translation="MPGLRDPLTMLVRRGREPAVVQTVRSTAAAVIAYVVALWLSNEPA
                     PLTAPLTALLVVQVTLYATLTTGVRRVNSVVAGVLIAIGFSVLVGLSWWSLGLIILASL
                     VIGRFVKAGEFVPEVAISAMLVLGVAQVAETAWGRVLETLIGAGVGLLFNVLLVPPVWV
                     QPAGEAIEDLARRMRHLLQDIGEEISGHIPVEEAAARLHAARRLDHDIVQVDASLRQAE
                     DSLRLNPRVKEGLLYRVVLRTGLDALEITAVVLRVTCRTLTDLATHRREESLFPEETAA
                     ALRKVFTNMALAVEHFAIVITTHVTTSAEHAEENLTRELDAARRTRDRAADLLLRQVQL
                     HPRQWQLHGALLAEIDRILDELDVEKRTERLTQELDRHSREQRERYPRLHRVAERLRKS
                     RYGRRSTLK"
     gene            4104..4877
                     /locus_tag="FGW37_RS31400"
                     /old_locus_tag="FGW37_31680"
     CDS             4104..4877
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (12-178). E-value: 1.2e-13.
                     Score: 43.3. Matches aSDomain:
                     nrpspksdomains_FGW37_RS31400_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999105.1"
                     /locus_tag="FGW37_RS31400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31680"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_179084170.1"
                     /sec_met_domain="adh_short (E-value: 3.8e-33, bitscore:
                     113.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1e-33, bitscore:
                     115.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEHQGFLAGKCILVTGASSGIGAAAAKVFAREGAAVVLTARREE
                     RLAVLCEEIRGGGGDAAFVVGDIRDEGDARRAVEFAVERHGRLDAAFNNAGIGWDQEPM
                     HLMKDDAYDAIMDTNVRGVWNVMRHELAAMVEAGSGGSVVNNSSVAGMVAIPAAAPYIA
                     SKHAVIGLTKAAAAEYAPHGIRVNTVAPGTTRSEIIAGWFERNPHIEPELHRLTPQGRT
                     AEPAEIAEAAAWLCSDRASFVTGAVLPVDGGFTMV"
     CDS_motif       4140..4232
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FGW37_RS31400_0001"
                     /evalue="1.00E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="FGW37_RS31400"
                     /protein_end="43"
                     /protein_start="12"
                     /score="15.0"
                     /tool="antismash"
                     /translation="ILVTGASSGIGAAAAKVFAREGAAVVLTARR"
     aSDomain        4140..4637
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FGW37_RS31400_PKS_KR.1"
                     /evalue="1.20E-13"
                     /label="FGW37_RS31400_PKS_KR.1"
                     /locus_tag="FGW37_RS31400"
                     /protein_end="178"
                     /protein_start="12"
                     /score="43.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="ILVTGASSGIGAAAAKVFAREGAAVVLTARREERLAVLCEEIRGG
                     GGDAAFVVGDIRDEGDARRAVEFAVERHGRLDAAFNNAGIGWDQEPMHLMKDDAYDAIM
                     DTNVRGVWNVMRHELAAMVEAGSGGSVVNNSSVAGMVAIPAAAPYIASKHAVIGLTKAA
                     AAE"
     gene            complement(5001..6245)
                     /locus_tag="FGW37_RS31405"
                     /old_locus_tag="FGW37_31685"
     CDS             complement(5001..6245)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="FGW37_RS31405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31685"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_179084171.1"
                     /sec_met_domain="AfsA (E-value: 1.9e-16, bitscore: 58.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQILLSPAPRADESTPQHADGAVPCRNVRLQADQIDASGRLRTS
                     AADLSAEDSRTGNGAGNGADAAGAGPGAGASGRIAKELVHLSFDDAVYLTGCEQQDATH
                     YTVSAVWPAHTAHRIPGHGTHYETLAVAQTIRQAGLFLAHTAYAAPLDHATLLRTFSYT
                     LNTDAAVDTHRATPLHINITATPTTTRNKRTTALHLHMTVTAAGSGSGDAGDADSETVL
                     ATGETDFEWIPPMVYRRLRGAYHRPPTTLPPVTPPLPPVQVGRTTPDEVVLTPTAQPRT
                     WQLRHTFDNTVLYDHAVDHVPGLVLIEAANQAAYTVTAPVTEAVTAGTDADTDADTDTD
                     TGPCACAPDTAGTGYRPQPTTTHTTFHRYAEFDAPLTVTTETRTTSHTVTHTTVGLQND
                     QPVFTTTHTAPAPVA"
     gene            6551..7216
                     /locus_tag="FGW37_RS31410"
                     /old_locus_tag="FGW37_31690"
     CDS             6551..7216
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047020.1"
                     /locus_tag="FGW37_RS31410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31690"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_179084172.1"
                     /transl_table=11
                     /translation="MAKQARAIHTRRLILEAAGEVFDEYGYESATIAEILGRAGVTKGA
                     LYFHFASKEELARGVLSEALTTEGVMPQESRLQEWVDVGMVLAHRLPREPLLSAAIRLS
                     ADRKARDLFGTSWPGWISFIAGQLAAAKEQGELLPHVDADRIAVLFVSAWTGVEVLSTA
                     LDGGDSLEDRVALLYDTILPGIAVPGALRALDTAPDRGTRVWDAHQAQREQEAASAAS"
     gene            complement(7726..8403)
                     /locus_tag="FGW37_RS31415"
                     /old_locus_tag="FGW37_31695"
     CDS             complement(7726..8403)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985133.1"
                     /locus_tag="FGW37_RS31415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31695"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_179084173.1"
                     /transl_table=11
                     /translation="MTKQERAMNTRHALIRSAAQAFDQFGYTRAKLAGISAGAGVSSGA
                     LHFHFDTKAAMAAAVESEAARTLHALARHAHRKSPSPLQALVDSSHVIAQQLRWDVVVR
                     AGFHLNCRTAHHSETNLRQEWLECVRRTLERSATHGQTPPQAVREELTAAIVGATAGFE
                     ALARHDRSWLSRHTLTGLWHTLLPQLATPKALTQLQPAGTESVINATGSTADRSAGADA
                     LRG"
     misc_feature    complement(8417..8429)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(8462..8480)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.3"
                     /tool="antismash"
     misc_feature    complement(8755..8768)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.13"
                     /tool="antismash"
     misc_feature    8819..8833
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            8930..9592
                     /locus_tag="FGW37_RS31420"
                     /old_locus_tag="FGW37_31700"
     CDS             8930..9592
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546904.1"
                     /locus_tag="FGW37_RS31420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_31700"
                     /product="response regulator transcription factor"
                     /protein_id="WP_343067211.1"
                     /transl_table=11
                     /translation="MRILVVDSDTRAAETLARRLQRHGCETFSVATGEAALAEYRSADL
                     VLLDPELPDLDGLEVCRAVRAASEVPIIAVTALAGELDRVLGLQAGADDYVVEPYGFRE
                     LMARIEAVMRRARPAARPAAEVITSGPLTIDPDLREVRLGGRRIDVTRKEFDLLHLLAS
                     RPEKVVSRKQLMTQVWDDRWSRPGRTIDTHVSSLRGKLGSAGWIITVRGVGFRFGHA"
     misc_feature    complement(9640..9654)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.83"
                     /tool="antismash"
     misc_feature    9733..9744
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    10159..10172
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 aagcgaactc cgcgatctgc ttgtcggaga ggttctgcgc gaggttggcc tccgagatca
       61 tgccgacgag gcggtggccg ttctgcacat cgatcaccgg cagccgcttg atctggtgct
      121 gctccatgga ctccagggcg tccgtcgcgc tcgcgtccgc gtcgatccag tgcagctccc
      181 cgccgagcga gcccgcctcc acactcgccg gatccacacc gtcggccacg cactggatga
      241 cgatgtcccg gtcggtgacc aggccctgga gcctgtcgtc gtcaccgcag atgggcagcg
      301 cgcccacgtg cagctcgcgc atcatcttcg ccgcgtcctg cagcgattcg tgctcgccga
      361 cgcacttcgc gcccgccgtc atgatgtcgc gggccttgag cggcttgttg cgtgccatga
      421 tcgctcctcc cgtcgtggcc ggttttcctt tctccatcct ccatcgcaac acggatggcc
      481 ccccgacgcc atgcgcgcgc acgctcactg cccgcggtcg gccatgtgct gttcgaacat
      541 gctgatcttt ccgtccagca tgccgggctt cccgggccac agatcggcct tcaggaccag
      601 ctggacccgt ggcgccctcg cggcgacggc ctccgtggcc cgccggacga cgtccatgac
      661 ctcctcccac tcgccctcga tcatggtgta catggaatcc gagcggtacg gcagcccgga
      721 ttcgcggatg attctcacgg cgtcggcgat ctcgtcggga aagctctctc cgacgccgac
      781 gggggtcagg gtgaaggcga tgagcacggt ggtccgccgt cctttctctt ctgtctcttc
      841 tctaactctt catctcattt gcccgcgggt catctcattt gcccgcgggc tgcggggcgg
      901 tcgtggtcgc gtgataggcg gacgcgacga gcaggagacg gtcgatctcg tgggtcaggc
      961 tgcgcgccga ggactcgtcc gcgtcccggt ccgtgggccc cgtgaggttc gccgtctggg
     1021 catggggctt gtggccgtcg tgcttgttcc gcagggcggc cgtgagccag gacgccgtga
     1081 tgtgcgcctc caccaggtgt tccggcaggg actgccgccc cacgctctga cggatggatt
     1141 cgagcgtggc cggcggaatg tagttgcgca ggacgatcat cgcgtcacgg atctcgatga
     1201 cccggcggta gagccgcagc tggaggggga cggccgccgc gccgatgcgg ttcgccgggc
     1261 gcaggacgac gtccggcgtg tgcgtggtca ggtcctgcca cagctgttcc aggccccggt
     1321 aggactttcg gtactgcagg tgggtccgga tccgcgcgaa caccggaagc gccactccga
     1381 gcgccatgaa cgccgcctcg acaccggtca cgggcgagaa cagcggcgcg aacaccggtg
     1441 aacgcgtaga caggtagacg agtttgagcg accacagcac ggaggccagc aggatgccgg
     1501 tgccgaacag gcgcagcccg tagcgcagga ggcgcggcgt gccgtgccgg ccgtaactcc
     1561 agcacacgaa tccgcagaag gtgtcggccg tgaggtggaa ggcgaagaac acccaccagt
     1621 aagcgtccgg cacgctcgcc gtgctccggg acggcgcgat ctgattgcgc gcgtgcgccg
     1681 gccccagcac gtccagcagc acgagggcgg ccatgacggc gaccgccgtg ccgaacagca
     1741 ggtgcgtgcg gcgatggcga ccggccgccc cgtggatgaa ccacaggacc gccgccgcat
     1801 cgacgatgct gaacagatgg gtggtcagat ccacccagtg cgccccgggg acgatgcgcg
     1861 cgagcacggc cgtcacgggt tcgaggtgca tggacatcgc cagggcgatc atgacgacgg
     1921 cgaaccacag gggccgctgc tcccggtgcc ggacggcctg cggggccctg acgaccgccg
     1981 ccgaccacag ggcggcgatg cccgcgttct ctatccaggt gagcacgaag cccccgcagg
     2041 acggcccacc cgcaggccca tcgccgattc caggccgccg agcatccccg acagcagcgt
     2101 cggcgccggc acccgcccgg ccgcctccag gatcagggtg gcgatcatct ccgcctcctg
     2161 ctcctgctcg tcgccgtagc ggatccgcgg caggacccgg cgcaccgagc ggggatcggt
     2221 gatgtcgaga tggcggtgga gccgggtgtc gtcgagcgcg gagcggtggt tgcacaccag
     2281 atgcccgatc tcgtggacga tgatgtgctc ctggtgcaac gggctggtct gcccgtcgta
     2341 gaggatgaag tcctgagtct cgacggacag cagcattccc gacggagtcc cggggacggt
     2401 cggtgtgggc agtgccagta gacgcagcgg ccgatcgcgt tcggccgcaa gggcttcgca
     2461 caggtcctgt gcggagaaag ggtccggcac aggaatccgg ccgatcacct tctggcatcg
     2521 gcggcgcaga tgcctccagt gcatctcagt cgcttctccc gcggtcgtag ggacgggcgg
     2581 gcacatgacc cggtagccgc gccgggtcat cgcggccccc cggagccggt ggtgggcggg
     2641 caggcccgca cggcatgctt caaggcggtc aatggcagta ccacgagccc ccctcgcaca
     2701 tatgcccaag ccgcctgcga tcgtagatcg ccgcgccgcg tcacgccagg gttgcggaac
     2761 gtcgccgggc tacttgagcg tgctgcgacg cccgtagcga ctcttgcgga gccgctcggc
     2821 gacgcggtgc aggcgcggat agcgctcgcg ctgctcccgg gagtggcggt cgagctcctg
     2881 cgtcagccgc tcggtgcgct tctccacgtc gagctcgtcg aggatgcggt cgatctccgc
     2941 gagcagcgct ccgtgcagct gccactgccg gggatgcagc tgcacctggc gcaggaggag
     3001 gtccgcggcg cggtcacggg tcctgcgggc ggcgtcgagt tcgcgcgtga ggttctcctc
     3061 ggcgtgttcg gcgctggtgg tgacgtgcgt cgtgatgacg atggcgaagt gctcgaccgc
     3121 cagggccatg ttcgtgaaga ccttccggag cgcggcggcg gtctcttcgg ggaagagcga
     3181 ttcctccctg cggtgggtcg cgaggtcggt gagggtccgg caggtcacgc gcagcaccac
     3241 cgcggtgatt tcgagtgcgt ccagcccggt gcgcagcacg acccggtaga ggagcccctc
     3301 cttcacacgc ggattgaggc ggagactgtc ctcggcctgc cgcagcgagg cgtccacctg
     3361 gacgatgtcg tggtcgaggc gccgggccgc gtgcagccgg gccgcggcct cctccacggg
     3421 gatgtggccg ctgatctcct caccgatgtc ctgcaagagg tggcgcatcc ggcgggcgag
     3481 gtcctcgatg gcctcccccg cgggctgcac ccacaccggg gggacgagca ggacgttgaa
     3541 cagcagcccg acgcccgcgc cgatcagggt ctccagcacc cggccccagg ccgtctccgc
     3601 gacctgcgcc accccgagga cgagcatcgc gctgatcgcg acctcgggga cgaactcgcc
     3661 cgccttcacg aaccgcccga tgacgaggga ggccaggatg atcagcccga ggctccacca
     3721 cgacagcccg accagcacgc tgaagccgat cgcgatcagg acgccggcca ccacggaatt
     3781 gacccggcgg acccccgtgg tgagcgtggc gtacagggtg acctggacga ccagcagcgc
     3841 ggtgagcggc gcggtcagcg gggccggctc gttgctcagc cacagcgcca cgacataggc
     3901 gatcaccgcg gctgcggtgg accggaccgt ctgcacgacc gcgggttccc tgccgcgcct
     3961 caccagcatc gtcagggggt cacgaagccc gggcacatcg gggttccttc cccttcccgc
     4021 ccgcctgcga gccatccgat gcgccactcg ggtggcggga ggggccgacc gggtctgcgc
     4081 agcgggatgg caggattccc gccatgactg aacatcaggg ttttcttgcc ggtaagtgca
     4141 ttctggtgac gggggcgagc agcggtatcg gtgctgccgc agcgaaggtg ttcgcgcgcg
     4201 agggcgccgc ggtggtgctg acggcgcgcc gggaagagcg cctggcggtg ctgtgcgagg
     4261 agatccgcgg gggcggtggc gacgccgcgt tcgtcgtggg cgacatacgg gacgaggggg
     4321 atgcgcgccg ggcggtggaa ttcgcggtgg agcggcacgg gcgcctcgac gccgcgttca
     4381 acaacgccgg aatcgggtgg gaccaggagc cgatgcacct gatgaaggac gacgcctatg
     4441 acgcgatcat ggatacgaac gtgcggggcg tctggaacgt gatgcggcac gaactcgcgg
     4501 ccatggtgga ggccgggtcg ggcggatccg tcgtcaacaa cagcagcgtc gccgggatgg
     4561 tggcgatacc cgccgccgcg ccgtacatcg cgtcgaagca cgcggtgatc ggcctgacga
     4621 aggcggcggc ggccgagtac gcgccgcacg gcatccgggt gaacacggtc gcgccgggga
     4681 cgacgcggag cgagatcatc gccggctggt tcgagcggaa cccgcacatc gagccggagc
     4741 tccaccgttt gacgccgcag gggcgcacgg ccgagccggc cgagatcgcg gaggctgcgg
     4801 cctggctgtg cagcgaccgg gcgtcgttcg tgacgggagc ggtgctgccc gtggacggcg
     4861 gattcacgat ggtgtgacgc tgcacggcgg tgtgacgcgg gcggagcccg gcggcctgat
     4921 ccggctgccg ggctccgccc gtgattccgc tgtggtgcgc cttgccttgc cttgctcgcc
     4981 tcgcccccgt ccccgtcccg tcaggcgacg ggtgcgggag cggtgtgcgt ggtggtgaag
     5041 acgggctggt cgttctggag cccgaccgtc gtgtgggtga cggtgtgcga ggtcgtacgg
     5101 gtctcggtcg tgacggtgag gggggcgtcg aactccgcgt accggtggaa cgtggtgtgc
     5161 gtggtggtgg gctgggggcg gtagccggtg cccgcggtgt ccggggcgca ggcgcacggg
     5221 cccgtgtccg tgtctgtgtc cgcgtccgtg tccgcgtccg tgcctgcggt gacggcctcg
     5281 gtgaccggtg cggtgacggt gtacgcggcc tggttggcgg cctcgatgag cacgagtccg
     5341 gggacgtggt cgacggcgtg gtcgtagagg acggtgttgt cgaaggtgtg gcgcagctgc
     5401 caggtgcggg gctgtgccgt gggggtgagc acgacctcgt cgggcgtggt gcggccgacc
     5461 tgcacgggcg ggagcggcgg cgtgacgggc ggcagcgtcg tgggcggccg gtggtacgca
     5521 ccccggaggc ggcggtagac catgggcgga atccactcga agtcggtctc gcccgtggcg
     5581 agaacggtct cggagtccgc gtcgcccgcg tcgccggagc cggagccggc ggcggtgacg
     5641 gtcatgtgga ggtggagggc ggtggtgcgc ttgttgcgcg tcgtggtggg agtggccgtg
     5701 atgttgatgt gcagcggcgt cgcccggtgg gtgtcgacgg cggcgtcggt gttgagggtg
     5761 taggagaagg tacgcaggag agtggcgtgg tccagcgggg ccgcgtaggc ggtgtgggcg
     5821 aggaagagac ctgcctggcg aatggtctgg gcgacggcga gggtctcgta gtgggtgccg
     5881 tggccgggta tgcgatgggc cgtgtgggcg ggccagaccg cggagacggt gtaatgggtg
     5941 gcgtcctgct gctcgcagcc ggtcaggtac acggcgtcgt cgaagctgag gtggaccagt
     6001 tccttggcga tgcggccgct ggcgccggcc ccggggccgg cgccagcggc gtcggcaccg
     6061 ttgccggcac cgttgccggt ccgcgagtct tcggcgctaa ggtctgcggc tgaggtgcgg
     6121 agccggccgg acgcgtcgat ctgatcggcc tgcagccgga cgttccggca cgggacggcc
     6181 ccgtcggcgt gctgcggggt gctctcgtcg gcacggggag cggggctgag caggatctga
     6241 gacacgtctc tcctctaagt ggattcgtgc gtcccggtgg ttagccggct cggtgataag
     6301 atacgtacta tccggtttgc ttttgggtga gtggcagggt atggagccga ccgcccggtg
     6361 gagccgggtg agaaagaccc cggattcgcc gcaccggggg cggaccgcag gttccgccgg
     6421 tgcgccggtg tcgtcgtgct tgctcaggcc gttgtccgcg ggccccgccc ggctcgtccg
     6481 gctgtgcccg gcgatcctgc cgggtgagcg gtcttgtagc ggttggctgt cggtgattgg
     6541 gaggtaatca atggcgaagc aggcacgggc gattcatacg cgccgtctca ttctcgaggc
     6601 ggcgggcgag gtcttcgacg agtacggata cgagtcggcg acgatcgcgg agatcctcgg
     6661 gcgcgcgggc gtgaccaagg gtgcgctgta cttccacttc gcctccaagg aggagctggc
     6721 gcgtggcgtc ctcagtgagg cgttgacgac cgagggcgtc atgccccagg agtcgcgcct
     6781 tcaggagtgg gtggacgtcg gcatggtgct ggcgcaccgg ctgcccaggg agcccctgct
     6841 gagcgccgcg atccggctgt cggccgaccg taaggcacgg gacctgttcg gtacgtcctg
     6901 gcccgggtgg atctccttca tcgcggggca gctggccgcg gcgaaggagc agggggagct
     6961 gctgccgcac gtcgacgcgg accggatcgc cgtgctgttc gtgagcgcct ggacgggcgt
     7021 cgaggtcctc tcgacggcgc tggacggggg cgactccctg gaggaccggg tcgcgctgct
     7081 ctacgacacg atcctgccgg gcatcgcggt gccgggtgcg ctgcgggcgc tggacacggc
     7141 ccccgaccgc gggacgcggg tctgggacgc gcaccaggcc cagcgcgagc aggaagcggc
     7201 atccgccgcg tcgtaacgac cgaggcggtt ccccggcggt gaggccgggt gggcaggaga
     7261 tgtgcgggcg gcgggctgtg caggaccgct gccgcatgga gggccggccc tccgcgctgc
     7321 cacggatgac gacgtccacg cttccccctg tgtttgtggc cgatgccggc gcgggcgctt
     7381 ggtgccgatg gacacgagcc cccctcgtca ctcccggcac ggcatcgaaa ggctgtcccg
     7441 gagccgctgc tcgtgacagc ggggcctcgg gtgatccgac gtcagggacg ctaacaaacc
     7501 ggccatcctg tttgtcaagg ctcgacggga atcccatact catccggtga cctgggtgaa
     7561 cgctccagcg ggaaggcgac cgggcgcagt tggaggagaa accgcatggt tggtttctct
     7621 attcccccaa gggaacggca aaggcccgca acgtgcattg cgggcctggt ggttggttgc
     7681 gcggtcgtga ctgctgtgct ccgtccgaag gggcgccgcg ggagctcagc cgcggagcgc
     7741 gtcggccccg gcggaccggt cggcggtgct gccggtcgca ttgatgacgg attccgtacc
     7801 ggcgggctgc agttgggtca gcgccttggg cgtcgccagc tgcggcagca gagtgtgcca
     7861 gagaccggtg agggtgtggc gtgagagcca gctgcggtcg tgccgcgcca gggcctcgaa
     7921 gccggcggtc gcccccacga tcgcggcggt cagctcctcc cgcacggctt gcggcggcgt
     7981 ctgtccgtgg gtcgcggacc gttccagcgt gcggcgtacg cactcaagcc attcctggcg
     8041 cagattcgtc tcggagtggt gcgcggtgcg gcagttgagg tggaagcctg cgcgcaccac
     8101 gacgtcccat cgcagctgct gggcgatgac gtgcgacgag tcgacgagcg cctgcaaggg
     8161 ggaggggctc ttgcggtggg cgtgccgggc cagggcgtgc agggtccggg cggcttcgga
     8221 ctccacggcc gcggccatgg ccgccttggt gtcgaagtgg aagtgcaggg cgcccgagct
     8281 caccccggcg ccggcgctga tgccggcgag cttcgcgcgg gtgtagccga actggtcgaa
     8341 ggcctgggct gcggagcgga tcaatgcgtg ccgggtgttc atggcccgtt cttgcttcgt
     8401 caccggcaac tcctggggga caacgtcatg gggcacgtca tgcgccacat gaaaccaact
     8461 aactggtttt atttctactg gcgctgtccg gtatgggtgt taagtgaaat atctggcgcc
     8521 gggaagcccc cacgggccgt gcctcccacg ttgcgcaccg tactgatcgg cggtgtggcg
     8581 caagggctca ccttcttgcg gatgccggta aatcggccta ctggggggca agttgcttta
     8641 atttttccgc ctgcgtcctt cattcgcgac cgaaatgcga cttctgttgg tgaaggtgcc
     8701 gtctcgccct attagcgctt tgacaaaccg acagcccggt ttgtaatgtc gcggcgaacg
     8761 gttgtccgcg gcgccgtcgc cgcgcgaacg tgcaggagca gcgatggatc agcaggtcag
     8821 cagccttctc gatgcgcacc cgggggaagc gccccggcgc cgtgtgccca ggccgagggc
     8881 cgggcatcct gcagaacggg ccccggccct gacacggccg ggcgaaacct tgcggatcct
     8941 cgtcgtcgac agcgataccc gagccgccga gacgcttgcc cggagactgc agcgccacgg
     9001 ctgcgagacc ttctccgtcg ccaccgggga ggccgcccta gccgagtacc gttcggccga
     9061 cctggtgctg ctggatccgg aactgcccga tttggacggc ctggaggtct gccgggccgt
     9121 gagagccgcc agcgaggttc ccatcatcgc ggtgaccgcc ctggccggag agctcgaccg
     9181 ggtcctcggc ctgcaggccg gtgccgacga ctacgtcgtc gagccctacg gcttccgcga
     9241 actgatggcc cgtatcgagg cggtcatgcg ccgtgcgcgc cccgcagcgc gccccgcggc
     9301 ggaggtcatc accagcgggc cgctgaccat cgaccccgac ctgcgcgaag tgcgcctcgg
     9361 gggacgccgg atcgacgtca cccgcaagga attcgacctg ctccacctgc tggcctcccg
     9421 ccccgagaag gtcgtctccc gcaaacagct catgacccag gtgtgggacg accggtggtc
     9481 ccggcccggc cggaccatcg acacccacgt cagcagcctg cggggcaagc tcgggtccgc
     9541 cggctggatc atcaccgtcc gcggcgtcgg cttccgcttc gggcacgcct gagcccgccc
     9601 ctcccgcaca cgagtgcctc gtcgatcacc actcgaccgc tctctgattg agtctgccga
     9661 catccgatga cttccgatcg tctttgaaag aggacattca tcgtgcgcaa ggtgctcatc
     9721 gccaaccgtg gcgaaatcgc tgtccgcgta gctcgcgcgt gccgggatgc cgggattgcc
     9781 agtgtggcgg tctacgcgga tccggaccgg gatgcgctgc atgtccgcgc cgctgatgag
     9841 gcgtatgcgc tgggcggtga caccccggcg acgagctacc ttgatgtggc caaggtgctt
     9901 gccgcggcgg ccgagtcggg ggcggatgcc gttcatcccg ggtacggctt cctttccgag
     9961 aacgccgagt tcgcgcaggc ggtgctggat gcgggtctga cgtggatcgg tccgccgccg
    10021 caggcgatcc gggacctggg tgacaaggtg gcggcccgtc atatcgctca gcgtgcgggt
    10081 gcgccgctgg tggccggtac tccggacccg gtctcgggtg cggaggaggt cgtggcgttc
    10141 gcccgcgagc acggtctgcc gatcgcgatc aaggcggcct tcggtggtgg cgggcgcggt
    10201 ctgaaggtcg cccgcaccct ggaagaggtc cccgagctct acgactccgc cgtccgcgag
    10261 gcggtcgccg ccttcgggcg cggggagtgc ttcgtcgagc gttacctcga ccggccccgc
    10321 cacgtcgaga cccagtgcct ggcggacaag cacggcaacg tcgtggtcgt ctccacgcgt
    10381 gactgctcgc tgcagcgccg ccaccaaaag ctcgtcgagg aggccccggc gccgttcttg
    10441 tcggcggagc agaacgccga gctctaccgc gcctcgaagg cgatcttgcg tgaggccggc
    10501 tacgagggcg cgggcacgtg cgagttcctc gtcggccagg acggcacgat ctccttcctg
    10561 gaggtcaaca cccgcctgca ggtcgagcac ccggtcaccg aagaggtcac cggcatcgac
    10621 ctggtccgcg agatgttccg catcgccgac ggcgaagagc tcggctacga cgacccgccg
    10681 ctgcgcggtc actccttcga gttccggatc aatggtgagg atgcgggccg taacttcctg
    10741 cccgctccgg gcacggtcac ccgcttcgac gccccgacgg gtccgggtgt gcgcctggat
    10801 gcgggggtgg aggcgggcag tgtcatcggc ccggcgtggg actcgctgct ggcgaagctg
    10861 atcgtcaccg gtgccacgcg tgagcaggcg ctgcagcggg cggcgcgtgc gctgagcgag
    10921 ttcaaggtcg agggcatggc cacggccatt cccttccacc gggccgtggt ggccgatccc
    10981 gctttcaccg ccgatccctt ccgggtccac acgcggtgga tcgagacgga gttcgtcaac
    11041 accatcgagc cgttcgcggg tgccgcggcc gaggaagagg gcgaggaggc cggccgcgag
    11101 accgtcgtcg tcgaggtcgg cggcaagcgc ctggaagtct ccctgccctc ttccctcggt
    11161 acgaccgtcg cccgcaccac ctcggccgga ggcacgaagc ccaagcgccg cgccgccaag
    11221 aagtccggct tcgcggtctc cggcg
//
