LOCUS       NZ_CP040752            10399 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces rectiverticillatus strain B15-08 chromosome, complete
            genome.
ACCESSION   NZ_CP040752
VERSION     NZ_CP040752.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces rectiverticillatus
  ORGANISM  Streptomyces rectiverticillatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7679243)
  AUTHORS   Park,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2019) Laboratory of Bioorganic Chemistry, NIDDK,
            NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2018
            Assembly Method                   :: HGAP v. Dec-2018
            Assembly Name                     :: DDK_LBC_B15-08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013394065.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:49:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,642
            CDSs (total)                      :: 6,543
            Genes (coding)                    :: 6,394
            CDSs (with protein)               :: 6,394
            Genes (RNA)                       :: 99
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 55 of 149
            Pseudo Genes (incomplete)         :: 125 of 149
            Pseudo Genes (internal stop)      :: 12 of 149
            Pseudo Genes (multiple problems)  :: 41 of 149
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:46:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1838495
            Orig. end                         :: 1848894
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040752.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from LBC, NIDDK.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="20"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            147..1097
                     /locus_tag="FGW37_RS07780"
                     /old_locus_tag="FGW37_07900"
     CDS             147..1097
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986841.1"
                     /locus_tag="FGW37_RS07780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07900"
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_179080390.1"
                     /transl_table=11
                     /translation="MPTPRIAPTAADRTARLAVTVFPLLVVAAGGVGLAAPSHFTGWAP
                     AVPWLLGVVMFTMGLTLTGEDFRAVAKRPWAVGLGLAAHYVIMPGLGWLIATVLGLPPQ
                     LAAGVILVGCAPSGTASNVVTYLARGDVALSVSVATVSTLVAPLVTPPLTLLLADRFLD
                     VDAGAMLTDILKTVLVPVLGGTLVRMAAGRWVAKVLRALPVLSALTIAVLVLIVVSGSA
                     SRIKDAAALVLLAVVLHNGLGLALGYAAGRLARLGRPSSRAMAFEVGMQNSGLAASLAT
                     AHFSPAAALPAAIFSVWHNVSGAALAAWMSRRPHP"
     gene            complement(1584..2684)
                     /locus_tag="FGW37_RS07785"
                     /old_locus_tag="FGW37_07905"
     CDS             complement(1584..2684)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="FGW37_RS07785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07905"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_179080391.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.3e-15, bitscore:
                     54.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSGVLHIKGRVLVGADPDGGVRDELWAVDGRITFERPVGARDV
                     TTVEGWALPGLVDAHCHVGLDAHGPVDESTAEKQALTDRDAGALLLRDAGSPSDTRWID
                     DREDLPRIIRAGRHIARTRRYIRNYAHEIEPDDLVAYVAREARRGDGWVKLVGDWIDRE
                     TGDLSFCWPRGEVAAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPRLADGGEGKFPRWADHMRRLHARRYETVRAAYDAGIPV
                     FVGTDAGGSLAHGLVGQEVDELVKAGLPPLAALSATTWSARSWLGRPGLEEGAPADIVV
                     YETDPRADVRVLGSPKRVILRGRVIR"
     misc_feature    2902..2917
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3074..3643
                     /gene="ectA"
                     /locus_tag="FGW37_RS07790"
                     /old_locus_tag="FGW37_07910"
     CDS             3074..3643
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="FGW37_RS07790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07910"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_179080392.1"
                     /transl_table=11
                     /translation="MTAAQADLARARPASLREFPEMPEDLTLDVPRVEDGAAIWRIARD
                     SGTLDLNSSYSYLLWCRDFAATSAVARRTGDGEPAGFITGYVRPARPRTLVVWQVAVDR
                     PHRGRGLAAALLEGLVTRVARESESGIDGIETTVTPGNTASNRLFASFAERHRAPVERE
                     VLFHGGLFPETGHDPEVLHRIGPLAH"
     gene            3691..4959
                     /gene="ectB"
                     /locus_tag="FGW37_RS07795"
                     /old_locus_tag="FGW37_07915"
     CDS             3691..4959
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 7.5e-73.
                     Score: 237.4. Matches aSDomain:
                     nrpspksdomains_FGW37_RS07795_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="FGW37_RS07795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07915"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_179080393.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-75, bitscore:
                     252.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRSWPAVFDRAQGSRMYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGVTHGLDMGTTAKRAFLEAFQDVVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDHYLDDQVPDFLWFERLLGDQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRGLAALCERHDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSIGGYGLPMS
                     LTLFRPELDVWEPGEHNGTFRGNNPSFVTAAAALDAYWTDGQMEKQTLARGEQAEEALR
                     AICAEHPELGAHYRGRGLVWGLEFADKTRASAVARRAFELGLLVETSGPEGEVLKLLPA
                     LTISPEELDEGLRIVARAVRETA"
     aSDomain        3775..4785
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FGW37_RS07795_Aminotran_3.1"
                     /evalue="7.50E-73"
                     /label="FGW37_RS07795_Aminotran_3.1"
                     /locus_tag="FGW37_RS07795"
                     /protein_end="365"
                     /protein_start="28"
                     /score="237.4"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMGTTAKRAFLEAFQDVVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDHYLDDQVPDFLWFER
                     LLGDQGSGLNTPAAVIVETVQGEGGINVARPEWLRGLAALCERHDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSIGGYGLPMSLTLFRPELDVWEPGEHNGTFRGNNPSFV
                     TAAAALDAYWTDGQMEKQTLARGEQAEEALRAICAEHPELGAHYRGRGLVWGLEFA"
     gene            5001..5399
                     /locus_tag="FGW37_RS07800"
                     /old_locus_tag="FGW37_07920"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="FGW37_RS07800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07920"
                     /product="ectoine synthase"
                     /protein_id="WP_058044967.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.3e-54, bitscore:
                     179.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQDIENTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTDDETGEKHWISPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            5403..6326
                     /gene="thpD"
                     /locus_tag="FGW37_RS07805"
                     /old_locus_tag="FGW37_07925"
     CDS             5403..6326
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436210.1"
                     /locus_tag="FGW37_RS07805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07925"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_179080394.1"
                     /sec_met_domain="PhyH (E-value: 5.9e-36, bitscore: 123.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAVTDLYPTRGTHEVAVPRRDPVVWPGSAPASGAGIGIGTGTPG
                     PIDAAGLAGYERDGFLTVDEMLAPDEVQVLRAELDRLIADPAVRADERAVVEPHSQDVR
                     SVFEVHRISEVFARLVRDPRVVGRARQILGSDVYVHQSRVNVKPGFGASGFYWHSDFET
                     WHAEDGLPRMRTVSVSIALTENLDTNGSLMIMPGSHRTFLGCAGVTPRDNYKRSLRMQE
                     AGTPSDSALAALAGRHGIRLFTGAAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVE
                     NTAVEPFAAPSRRPDFVGARDFTPVG"
     gene            complement(6372..7412)
                     /locus_tag="FGW37_RS07810"
                     /old_locus_tag="FGW37_07930"
     CDS             complement(6372..7412)
                     /NRPS_PKS="Domain: Aminotran_5 (13-341). E-value: 2.5e-27.
                     Score: 87.6. Matches aSDomain:
                     nrpspksdomains_FGW37_RS07810_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059809.1"
                     /locus_tag="FGW37_RS07810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07930"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_179080395.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.1e-20, bitscore:
                     71.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAPAHFAPETVYLNTAATGLVPAPAVAAMTEAATAFSLGRPG
                     AEDHYPVVEAVRAAFARLVGVPARQVATGGSAAVFCGLVAQSLPEGSEVLCAEGDFSSL
                     VNPFHVRPGLRVRTVPLEKIADAVGPGTALVAVSAVQSADGRLAGLGAIGEAARAHGAR
                     TLVDVSQAAGWLPVDAGAHDFTVCVGYKWLMGPRGTAYLTVPEDLGGLTPVFAGWVAGE
                     DPWNSTYGQVSLARSARRYDENPALLPYTGALAGLRLVEELGTDRIAAHDRALAGRFRT
                     GLAALGHRPAPAEDSAVVSVPGLGHAAERLAAAGVRVSARAGGLRASFHLYNSSSDVDR
                     VLEALV"
     aSDomain        complement(6390..7373)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FGW37_RS07810_Aminotran_5.1"
                     /evalue="2.50E-27"
                     /label="FGW37_RS07810_Aminotran_5.1"
                     /locus_tag="FGW37_RS07810"
                     /protein_end="341"
                     /protein_start="13"
                     /score="87.6"
                     /tool="antismash"
                     /translation="VYLNTAATGLVPAPAVAAMTEAATAFSLGRPGAEDHYPVVEAVRA
                     AFARLVGVPARQVATGGSAAVFCGLVAQSLPEGSEVLCAEGDFSSLVNPFHVRPGLRVR
                     TVPLEKIADAVGPGTALVAVSAVQSADGRLAGLGAIGEAARAHGARTLVDVSQAAGWLP
                     VDAGAHDFTVCVGYKWLMGPRGTAYLTVPEDLGGLTPVFAGWVAGEDPWNSTYGQVSLA
                     RSARRYDENPALLPYTGALAGLRLVEELGTDRIAAHDRALAGRFRTGLAALGHRPAPAE
                     DSAVVSVPGLGHAAERLAAAGVRVSARAGGLRASFHLYNSSSDVDRV"
     misc_feature    7638..7656
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.4"
                     /tool="antismash"
     misc_feature    complement(7639..7657)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.55"
                     /tool="antismash"
     gene            7648..7839
                     /locus_tag="FGW37_RS07815"
                     /old_locus_tag="FGW37_07935"
     CDS             7648..7839
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FGW37_RS07815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FGW37_07935"
                     /product="hypothetical protein"
                     /protein_id="WP_179080396.1"
                     /transl_table=11
                     /translation="MTSTTTVPDDHATKRRVLRAAAILTDAAATALAYAVQVIIGPLVL
                     AAGGARFFVRRSARAAAL"
     gene            7836..9119
                     /locus_tag="FGW37_RS07820"
                     /old_locus_tag="FGW37_07940"
     CDS             7836..9119
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002232.1"
                     /locus_tag="FGW37_RS07820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07940"
                     /product="histidine kinase"
                     /protein_id="WP_246430306.1"
                     /transl_table=11
                     /translation="MTALLRRGAVALVGGRARLRWVHLLLGGALLMPYYLLASTLTAVF
                     VSDTNILFPSSLGRQFGVFALALVPAAVTALVPFVRTLESAAARALCGVPEEALATGPG
                     ESRAARGRTAVWYTLHLLAGGIVSGMSLATPPFALMLIALPFTGVPGGEDAPYGLGNLA
                     FPWTLLSVPAGLAVLVLLAGVSGGAGALLARCAPVLLGPTAADRLAEAEQHAMRLALRN
                     RLARELHDSVGHALSAVTLQASAARKVLDADPEFARQALAAIEETTREAVGELDTVLGL
                     LREDGAASAAPAPTLADLDGLLERTRAAGGTVTLAASPALDGVPPVVSREAYRIVQEGL
                     TNALRHAGRVPVLLRITVHDDAVEVALENPVNPDKAPGRAHRRTGGGRGLTGIAERARL
                     LRGSSSSGERDGVWRLAVRLPLSGGAGR"
     gene            9116..9781
                     /locus_tag="FGW37_RS07825"
                     /old_locus_tag="FGW37_07945"
     CDS             9116..9781
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718906.1"
                     /locus_tag="FGW37_RS07825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_07945"
                     /product="response regulator transcription factor"
                     /protein_id="WP_179080397.1"
                     /transl_table=11
                     /translation="MTAPLRIVLADDERMVRTALRAILGAEEDLEVVGEATTGAEAVSV
                     VRELRPDVVLMDVRMPEVDGIRATEQLLGGMDSPPRVIVVTTFENDSYVYDALRAGASG
                     FLLKRAGADELVQAVRLVARTDSLLFPAAVRALAAEHAEARRARAAAGKLRAQLSDREA
                     EVLRLMARGLSNAEIAEQLVLGAATVKTHVAGALAKLGVRNRTQAVIAAYECGFVSPR"
     misc_feature    complement(10152..10166)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
ORIGIN
        1 agggtgaagg ggcgccctcc tgaggtcagt tcaccccaac gggttgtttc cgcaacggct
       61 ttcgattcgc tgggcgcggg agttgatcct tgtcactggc gtcacccctg ttcgggaggc
      121 gggcttgatc gctaaggtgc cgacgtatgc cgaccccgcg gatagccccg acagccgccg
      181 accggacggc gcggctggct gtcaccgtgt tccccctgct cgtcgtcgcc gccggagggg
      241 tggggctcgc cgccccctcc cacttcaccg gctgggcgcc ggccgtgccc tggctgctgg
      301 gcgtcgtgat gttcacgatg ggcctcacgc tcaccggcga ggacttccgc gccgtcgcca
      361 agcggccctg ggcggtcggg ctcggcctcg ccgcccacta cgtgatcatg ccggggctgg
      421 gctggctgat cgccacggtg ctcgggctgc cgccgcagct ggccgccggg gtgatcctcg
      481 tcggctgcgc gccgagcggc acggcgtcca acgtcgtcac gtacctcgcg cgcggggacg
      541 tcgccctgtc cgtgtccgtc gccaccgtct ccacgctcgt cgcgccgctc gtcaccccgc
      601 cgttgacgct gctgctggcg gaccggttcc tggacgtgga cgcgggcgcg atgctcaccg
      661 acatcctcaa gaccgtgctc gtgccggtgc tcggcgggac gctcgtgcgg atggccgcgg
      721 ggcggtgggt cgccaaggtg ctgcgcgccc tgccggtgct ctcggcgctg acgatcgccg
      781 tcctcgtcct catcgtggtc tcgggcagcg cgtcccgcat caaggacgcc gccgcgctgg
      841 tcctcctcgc cgtcgtcctc cacaacggcc tcggcctggc cctcggctac gcggcgggcc
      901 ggctggcccg gctcggccgc ccgtcgagcc gggcgatggc cttcgaggtc ggcatgcaga
      961 actcgggcct ggcggcgtca ctggccacgg cccacttcag cccggcggcg gcgctgccgg
     1021 cggcgatctt ctcggtatgg cacaacgtct cgggcgcggc gctagcagcc tggatgtcaa
     1081 ggcgccccca cccctagcgg tctgcaccgc catgacacgc cccccggcgt cgccgagccg
     1141 accgcaggcc gtcaggcacc gccgcgaacc accatcgtgg cgcatcgtcg ccgcggcgga
     1201 ggaagacgcc ggcctcaggc ccgcacaccg accggtgcgg ctcaccgccc ccggcgggtg
     1261 cccggccggc cccaggcagg ccaggcaccg ccggacacca ccaccgtggc cgatcaccgc
     1321 cgcggcggag gaagttgccc ggcccgcagc cacaccggaa gcaacccgcc acaccacccg
     1381 ggccacgccc ctcttccccg ccgccacggc gcgcaacccc caccaaacgc acccggcggt
     1441 gcccagccga ccccaggcag gccaggcacc gccggaaacc atcaccgtgg cggaacacca
     1501 ccgcggcgga ggaagacgcc gaccccggcg acacacactg aaccccgggc cccgggccaa
     1561 gccgaccgca ggtcaccccg tggctagcgg atcaccctcc cccgcagaat cacccgcttc
     1621 ggcgagccca ggactctcac gtccgccctc gggtccgtct cgtagacgac gatgtcggcc
     1681 ggggcgccct cctccaggcc cgggcggccg agccaggacc gggccgacca cgtggtggcc
     1741 gacagggccg cgagcggggg caggccggct ttgaccagtt cgtctacctc ctggcctacc
     1801 aggccgtgcg caagagagcc gcccgcgtcc gtgccgacga agaccgggat gcccgcgtcg
     1861 tacgccgccc gcaccgtctc gtagcgccgg gcgtgcaggc gccgcatgtg gtccgcccag
     1921 cgcgggaact tcccttcgcc gccgtccgcg agccgcggaa acgtcgcgat gttgacgagg
     1981 gtcggcacga tggcgacccc gcgctcggcg aacagcggaa tggtctcctc ggtgagcccg
     2041 gtggcgtgct cgatgcagtc gatgcccgct tccacgagcg gcgccagcga ctcctccgcg
     2101 aagcagtgcg cggtcacgcg cgccccgagg cggtgcgcct cggcgatcgc ggccgcgacc
     2161 tccccgcgcg gccagcagaa ggacaggtcg cccgtctcgc ggtcgatcca gtccccgacg
     2221 agcttgaccc acccgtcccc gcgccgcgcc tcccgcgcca cgtacgcgac gaggtcgtcc
     2281 ggctcgatct cgtgcgcgta gttgcggatg taccggcggg tgcgcgcgat gtggcgcccg
     2341 gcacggatga tgcgcggcag gtcctcgcgg tcatcgatcc agcgcgtgtc cgagggggag
     2401 cctgcgtcgc gcagcagcag cgctcccgcg tcccggtcgg tgagcgcctg cttctcggcg
     2461 gtggattcgt cgaccggacc gtgggcgtcg aggcccacgt ggcagtgcgc gtccacgagg
     2521 ccgggcagcg cccagccctc gacggtggtg acgtcccgcg cccccaccgg ccgttcgaag
     2581 gtgatccggc cgtcgacggc ccacagctcg tcgcgcaccc cgccgtccgg gtccgcgccg
     2641 acgagcaccc gccccttgat gtgcagcacc ccactgtcgc tcatggaccg cactgtacga
     2701 gcccgctcct cccgcccgcc aaggggccgt cgccggggcg ctgccggggt ggcgtccagc
     2761 cgcgtagggc aattccccgg aactcctctc cagtaattcc ggagacaact ctccgcgcat
     2821 tctcatgccc gctttcccgc accctaaacg cagcattttc cttacgcttc agcaagcgaa
     2881 ttcacgaccc ccgcgatcgc ctgtgacgca ctccacacag cccgcgattt gcccggatat
     2941 taacgggcaa actatcccat cccccacccg ctttgcgcgc ccccgcgcct ccgcgcgata
     3001 acacaaccac ctgcctccgg gtaacccgga cccccggtgc gatttccgat ccgcatcggt
     3061 aaattccctc cgcatgaccg cagcacaagc agaccttgca cgtgcccgtc ccgcctccct
     3121 ccgggaattc ccggaaatgc cggaggacct cacgctcgac gtcccgcgcg tcgaggacgg
     3181 agccgcgatc tggcgcatcg cccgcgactc gggaaccctg gacctcaact cctcgtacag
     3241 ctacctgctg tggtgccgcg acttcgcggc cacctcggcc gtggcccgcc gcaccggcga
     3301 cggggagccc gccggcttca tcaccggcta cgtccggccc gcccgccccc gcaccctcgt
     3361 ggtgtggcag gtcgccgtgg accgcccgca ccggggccgc ggcctggccg ccgcgctgct
     3421 ggagggcctc gtcacacggg tcgcccgcga gagcgagagc ggcatcgacg ggatcgagac
     3481 caccgtcacg cccggcaaca ccgcctccaa ccgcctgttc gcctccttcg ccgagcggca
     3541 ccgcgccccc gtggagcgtg aagtcctctt ccacggcggg ctgttccccg agacggggca
     3601 cgaccccgag gtcctgcacc gcatcggccc cctcgcgcac tgagacatct cgcccactga
     3661 gaaccctcac gcaccgacag gagcaccccc atgaccatca ccccgcccgc actcagcgtc
     3721 ttcgaggccc tggagtcgga ggtgcgcagc tactgccgca gctggcccgc ggtcttcgac
     3781 cgggcccagg gcagccggat gtacgacgag gacggccaca cctacctcga cttcttcgcc
     3841 ggcgccgggg cgctcaacta cgggcacaac aacccggtcc tcaaacgcgc cctgatcgat
     3901 tacatcgaac gcgacggcgt cacccacggc ctggacatgg gcaccaccgc caagcgcgcc
     3961 ttcctggagg ccttccagga cgtcgtgctg cgcccgcgcg acctgccgta caaggtgatg
     4021 ttccccggcc cgacgggcac caacgccgtg gaggccgcgc tgaagctggc ccgcaaggtc
     4081 aagggccgcg aggcgatcgt gtccttcacc aacgccttcc acggcatgtc gctgggctcg
     4141 ctcgccgtca ccggcaacgc cttcaagcgg gccggcgcgg gcatccccct ggtgcacggc
     4201 acgccgatgc cgttcgacca ctacctcgac gaccaggtgc cggacttcct gtggttcgag
     4261 cggctgctgg gagaccaggg ctcgggcctg aacacccccg ccgccgtcat cgtcgagacg
     4321 gtgcagggcg agggcggcat caacgtggcc cgccccgagt ggctgcgcgg gctcgccgcg
     4381 ctgtgcgagc gccacgacat gctgctgatc gtggacgaca tccagatggg ctgcggccgc
     4441 accggcgcct tcttctcgtt cgaggaggcg ggcatcaccc cggacatcgt gacgctctcg
     4501 aagtccatcg gcggctacgg cctgcccatg tcgctcactc tcttccgccc ggagctcgac
     4561 gtctgggagc ccggcgagca caacggcacg ttccgcggca acaacccctc cttcgtcacc
     4621 gccgccgcgg cgctcgacgc gtactggacc gacgggcaga tggagaagca gaccctcgcc
     4681 cgcggcgagc aggccgagga ggcgctgcgc gcgatctgcg ccgaacaccc ggagctgggc
     4741 gcgcactacc ggggccgcgg gctcgtgtgg ggactggagt tcgccgacaa gacccgggcc
     4801 tcggcggtcg cccggcgggc gttcgagctc ggcctgctcg tggagacatc gggccccgag
     4861 ggcgaggtcc tcaagctgct gcccgccctg acgatctccc ccgaggagct ggacgagggc
     4921 ctgcggatcg tcgcccgcgc ggtccgcgag acggcctgac ggccctgacc accgcaagta
     4981 ccgaaagaaa ggaacaaccc gtgatcgtcc gttccctcca ggacatcgag aacaccgacc
     5041 gccacgtcaa ggccaagtcc ggtacgtggg agagcaagcg gatcgtgctc gccaaggagc
     5101 gggtcggctt ctccctgcac gagacggtcc tctacgccgg cacggaaacc tccatgtggt
     5161 acgccaatca catcgaggcc gtgctgtgcg tggagggcga ggccgagctc accgacgacg
     5221 agaccggcga gaagcactgg atctcccccg gcacgatgta cctgctcgac ggccacgagc
     5281 ggcacaccct gcgccccaag accgacttcc gctgcgtgtg cgtcttcaac ccgccggtca
     5341 cgggacggga ggaccacgac gagaacggcg tctacccgct gctgacggag gagggctgaa
     5401 gcatggccgc ggtcaccgac ctctacccca cgcgcggcac gcacgaggtg gccgtcccgc
     5461 gccgtgaccc cgtggtgtgg cccgggtccg cacccgcctc cggtgccggc atcggcatcg
     5521 gcaccggcac gcccgggccc atcgacgcgg ccggtctcgc gggctacgaa cgcgacgggt
     5581 tcctcaccgt cgacgagatg ctcgcaccgg acgaggtgca ggtcctgcgc gccgagctcg
     5641 accggctgat cgccgacccg gcggtgcggg ccgacgagag agcggtcgtg gaaccgcact
     5701 cgcaggacgt gcggtcggtg ttcgaagtgc accggatcag cgaggtgttc gcacggctcg
     5761 tgcgcgatcc gcgcgtggtg gggcgggcgc ggcagatcct cggctcggac gtctacgtcc
     5821 accagtcgcg ggtcaacgtc aagccgggct tcggggcctc cgggttctac tggcactcgg
     5881 acttcgagac ctggcacgcg gaggacgggc tgccgcggat gcggacggtg tcggtgtcga
     5941 tcgcgctgac ggagaacctc gacaccaacg gcagcttgat gatcatgccg gggtcgcacc
     6001 ggacgttcct gggctgcgcg ggcgtgacgc cgcgggacaa ctacaagcgg tccctgcgga
     6061 tgcaggaggc cggcacgccg tcggacagcg cgctcgccgc gctggccggg cggcacggca
     6121 tccggctctt cacgggcgcg gccggttccg cgacgtggtt cgactgcaac tgcatgcacg
     6181 ggtccggcga caacatcacg cccttcccgc gcagcaacgt cttcatcgtc ttcaacagcg
     6241 tggagaacac ggcggtcgag ccgttcgcgg ccccgtcccg ccgcccggac ttcgtggggg
     6301 cgcgggactt cacgccggtg gggtaaccgg tccgggcggt gacgcggcgg ggccgccgcg
     6361 tcaccgctcg gtcagaccag cgcctccagg acgcggtcca cgtcggacga gctgttgtag
     6421 aggtggaagg acgcccgcag ccctccggcg cgggcgctga cgcgtacgcc tgccgcggcg
     6481 aggcgctccg ccgcgtggcc gaggccgggg accgagacga cggcggagtc ctccgccgga
     6541 gcgggccggt gcccgagcgc ggcgagcccc gtgcggaagc ggccggcgag ggcccggtcg
     6601 tgcgcggcga tccggtccgt cccgagttcc tcgacgagcc ggaggccggc cagggcaccg
     6661 gtgtagggca ggagggcggg gttctcgtcg tagcggcggg ccgagcgggc gagcgagacc
     6721 tgcccgtagg tgctgttcca ggggtcctcc cccgccaccc acccggcgaa gacgggggtg
     6781 aggccgccga ggtcctcggg gacggtgagg taggccgtcc cgcggggccc catcagccac
     6841 ttgtagccga cgcagacggt gaagtcgtgc gcgcccgcgt cgacgggcag ccagcccgcg
     6901 gcctggctga cgtcgacgag cgtacgggcg ccgtgggcgc gggcggcctc gccgatcgcc
     6961 ccgaggccgg cgaggcggcc gtcggccgac tggacggcac tgacggcgac gagcgcggtc
     7021 ccgggcccca ccgcgtccgc gatcttctcc aggggaacgg tgcggacgcg gagcccgggc
     7081 cgcacgtgaa aggggttgac gagggagctg aagtccccct ccgcgcagag gacttccgat
     7141 ccctcgggca acgactgggc gacgagcccg cagaagacgg cggccgaacc cccggtggcg
     7201 acctgccggg cgggcacgcc gacgagccgg gcgaaggcgg cgcggacggc ctccacgacc
     7261 gggtagtggt cctccgcacc agggcggccc agcgagaacg cggtggcggc ctcggtcatg
     7321 gcggcgacgg cgggcgcggg cacgagcccg gtggcggcgg tgttgaggta gacggtctcg
     7381 ggcgcgaagt gtgcgggggc caggctctcc atgcccaccc actatgcgcg tccggcgatc
     7441 gacccgtcca ccagtccgcc catcagtccg gccatcagcc cgttccacca gtccgtccgg
     7501 tcgtccctac cggtggtcca tccaccggtc tgtccatgcg ggcctcccgt cccctgcctg
     7561 agaactagcc gcccctcccc ctctcggcgg aggaagtccg ccggtcagag cgacgcgccg
     7621 gcgcccgccg gacggaacgc tggtcacatg accagtacga cgaccgtccc ggacgaccac
     7681 gccaccaagc gccgcgtgct ccgcgcggcg gccatcctga cggacgccgc ggcgacggct
     7741 ctcgcctacg ctgtgcaagt gatcatcggt ccgctcgtcc tcgccgccgg cggcgcacgc
     7801 ttcttcgtcc ggcgctccgc ccgggccgcc gcgctgtgac ggctctcctc cggcgcggtg
     7861 cggtcgccct ggtgggtggc cgcgcccggc tgcggtgggt gcacctgctg ctcggcgggg
     7921 cgctgctcat gccgtactac ctgctggcga gcacgctgac ggcggtcttc gtcagcgaca
     7981 cgaacatcct cttcccctcc tcgctcggcc ggcagttcgg cgtcttcgcg ctggcgctgg
     8041 tgccggcggc ggtgaccgcg ctggtgccct tcgtgcggac gctggagtcg gcggcggcac
     8101 gcgccctgtg cggggtgccg gaggaggcgc tcgcgacggg cccgggcgag tcgcgggccg
     8161 cgcgcgggcg gaccgccgtc tggtacaccc tgcacctgct ggccggcggc atcgtgagcg
     8221 gcatgtcgct ggccacgccc ccgttcgcgc tgatgctcat cgccctgccg ttcaccggcg
     8281 tccccggcgg cgaggacgcc ccgtacgggc tgggaaacct ggccttcccg tggacactgc
     8341 tgtcggtgcc cgccggcttg gcggtgctgg tcctgctcgc cggggtgtcg gggggcgcgg
     8401 gcgcgctcct cgcgcgctgc gcgcccgtcc tcctcggccc gacggccgcc gaccggctgg
     8461 ccgaggccga gcagcacgcc atgcggctgg ccctgcgcaa ccggctggcg cgcgaactgc
     8521 acgactccgt ggggcacgcg ctgagcgcgg tcaccctgca ggcgagcgcg gcgcgcaagg
     8581 tcctcgacgc cgacccggag ttcgcgcggc aggcgctggc cgcgatcgag gagacgacgc
     8641 gcgaggcggt cggcgaactg gacaccgtcc tggggctgct gcgcgaggac ggcgccgcgt
     8701 ccgccgcgcc ggcgccgacc ctcgccgacc tcgacgggct gctggagcgc acccgcgccg
     8761 cgggcggcac cgtcaccctc gccgcatctc cggccctgga cggcgtgccg ccggtcgtct
     8821 cccgcgaggc gtaccggatc gtgcaggagg gcctcaccaa tgccttgcgg cacgcgggcc
     8881 gggtgcccgt gctgctgcgg atcaccgtgc acgacgacgc cgtggaggtc gccttggaga
     8941 atccggtcaa ccccgacaag gcccccggcc gcgcgcaccg ccgcaccggc ggcggccgcg
     9001 gtctgacggg catagccgaa cgggcccggc tgctgcgcgg cagcagcagc tccggcgagc
     9061 gggacggggt gtggcggctc gccgtccgcc tgccgctgag cgggggtgcg ggccggtgac
     9121 ggcgcccctg cggatcgtgc tcgccgacga cgagcgcatg gtgcggaccg ccctgcgggc
     9181 gatcctgggc gccgaggagg acctggaggt cgtcggcgag gccacgaccg gcgcggaagc
     9241 ggtctccgtc gtccgcgaac tgcgccccga cgtcgtcctg atggacgtac ggatgcccga
     9301 ggtcgacggg atccgggcca ccgagcagct gctcggcggc atggactccc cgccgcgcgt
     9361 catcgtcgtc accacgttcg agaacgacag ctacgtgtac gacgcgctgc gcgccggggc
     9421 gagcggcttc ctcctcaaac gggcgggcgc cgacgaactc gtccaggccg tccggctcgt
     9481 ggcccgcacg gactcgctgc tcttccccgc cgccgtgcgc gccctcgcgg cggaacacgc
     9541 ggaggcccgc cgggcgaggg cggccgcggg caagctgcgc gcgcagctgt cggaccggga
     9601 ggcggaggtg ctccgcctga tggcccgggg cctgtcgaac gccgagatcg ccgaacagct
     9661 cgtcctgggc gcggcgacgg tgaagaccca cgtggcaggc gccctggcga aactgggcgt
     9721 ccgcaaccgc actcaagcgg tcatcgcggc ctacgaatgc ggtttcgtgt ccccgcgctg
     9781 agagcgcggg cgggcacccg ggcccttact tttgccgctg cggcggaagg aagggcccgg
     9841 cggggtcagc ggcccaccgc ctcgccgacc gtcctcacca ggcgcgtgac ctgcgcgtcg
     9901 ttgtcctcgc ggaagccgta ccggcggcgg ttgaagccga aggccaggcc gttggacggg
     9961 tcggcgaagg cctgggagcc ggcggcgccg ccgtggccga aggcgcgggc gccgaggaag
    10021 gggtacttgt cccccatggc accgaagccc aggccgaagg ccatgtgctg gcgcaggacc
    10081 gcgtcgtggc cgatggagtg gatctgggcg aagtcggcgg cggtggcggg cgtcagcagc
    10141 gcctccttgc cgtcgacgcc gctgatggcg gccgcgtaca tgcgggccag gccgcgcgcg
    10201 gaggccatgc cgccgacgga ggcggggccg cccgcgcgga tgacgcggtg gttcggcaga
    10261 tcctccagaa ccgtgggctg cgggtggttg aggttgaagg cgatgcccat caggctctgc
    10321 gggcccgagg cgccctccag cagggcgcgc ttctggccgg gcgtcgggtc catgggctgg
    10381 atgtcgagga cgcggtgct
//
