LOCUS       NZ_CP040752            11392 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces rectiverticillatus strain B15-08 chromosome, complete
            genome.
ACCESSION   NZ_CP040752
VERSION     NZ_CP040752.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces rectiverticillatus
  ORGANISM  Streptomyces rectiverticillatus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7679243)
  AUTHORS   Park,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAY-2019) Laboratory of Bioorganic Chemistry, NIDDK,
            NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2018
            Assembly Method                   :: HGAP v. Dec-2018
            Assembly Name                     :: DDK_LBC_B15-08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013394065.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 03:49:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,642
            CDSs (total)                      :: 6,543
            Genes (coding)                    :: 6,394
            CDSs (with protein)               :: 6,394
            Genes (RNA)                       :: 99
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 55 of 149
            Pseudo Genes (incomplete)         :: 125 of 149
            Pseudo Genes (internal stop)      :: 12 of 149
            Pseudo Genes (multiple problems)  :: 41 of 149
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:46:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1235621
            Orig. end                         :: 1247013
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040752.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from LBC, NIDDK.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11392
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11392
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11392
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6392](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6392
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    109..119
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(496..511)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.68"
                     /tool="antismash"
     misc_feature    1181..1195
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.9"
                     /tool="antismash"
     misc_feature    1267..1280
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.27"
                     /tool="antismash"
     gene            complement(1344..3242)
                     /locus_tag="FGW37_RS04950"
                     /old_locus_tag="FGW37_05025"
     CDS             complement(1344..3242)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547891.1"
                     /locus_tag="FGW37_RS04950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05025"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_246430272.1"
                     /transl_table=11
                     /translation="MTNAAAPSIPASAPAAAAQPDLDLGWDDPHFHRVASASLRQMAGR
                     LPAVLGRAARLGWAADRAATVQILVAQCVAAAAAAVALAATARVMAGLFGPGTAAEHLY
                     AALPALTVAAAAAAVRACADATAQYAALRLAPEVTALADERILADTPEVELTAYDRPDF
                     NDALQAAGTGAQATQDLITDAQVLLSAVAQLIAAAVVLTALHPLLLPLLALAALPKGAA
                     AVAAARIAHRAAHTSLADDRLRYLLRRFSTEPRTAAEIRAGTMAPYLRQWYAHLTDRIR
                     TRHRDAAPRVLLVTLCGAAGSGTMLALTWLTVGALVLGGAMGLAVAATAVVAVRASTAA
                     LTSLVMAGARLFRTSLYLQDWQDFLDLAEELRAVRGPQRLGAGGPREIRAREVSYRHPG
                     APSGRPALDRVSFTLRSGELVALVGENGSGKTTLSRLLTGLYLPTAGQVTWDGTDLAET
                     DPQDVWSRVGLVPQDYTRWPMAARENITLGQPRGGDDLVRTAARAAGADSVLDGLPGGL
                     DTLLAKSFLGGHDLSGGQWQRFAVARAFYREAAVLVLDEPTSAMDPRAEHTVISRFKEL
                     AAGRAAVFVTHNLTNTRIADRIVVLAEGRIVEEGTFEELAVGGGLFAELYKLSQDR"
     gene            3473..3724
                     /locus_tag="FGW37_RS04955"
                     /old_locus_tag="FGW37_05030"
     CDS             3473..3724
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FGW37_RS04955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FGW37_05030"
                     /product="hypothetical protein"
                     /protein_id="WP_179079940.1"
                     /transl_table=11
                     /translation="MSHSRSAARSRPSWSRTVGTDREPGVEGVRFSEVRQGEDAGRPVV
                     VAQGREAAVAGAQGGRVARVPKGNEARSKPGSCSAVVS"
     gene            complement(4069..4809)
                     /locus_tag="FGW37_RS04960"
                     /old_locus_tag="FGW37_05035"
     CDS             complement(4069..4809)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (extras)
                     Sulfotransfer_4: Sulfotransferase domain"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /locus_tag="FGW37_RS04960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05035"
                     /product="sulfotransferase family protein"
                     /protein_id="WP_179079941.1"
                     /transl_table=11
                     /translation="MTPDRAVRPGSVPHPGSVPRPGPLLPAARPKIFGIGLSRTGTRSL
                     TVALRTLGFDVAHYPADRGTYAALLAGTARFPLLDHRDGITDITVVPYYQDLDRAWPGA
                     KFILTVREEEGWLASCRTHWKRAIDGKTGRSEVYPQLQRFLRAAVYASHEFDPERFRTV
                     ARRHTAEVLRHFAGRPGDLLVLDITAGQGYERLAPFLGVPAPGDPFPHSDDYARVPAEG
                     PAAKGAAGPPATPAGPATPAGPAR"
     misc_feature    complement(4991..5005)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            5001..6392
                     /locus_tag="FGW37_RS04965"
                     /old_locus_tag="FGW37_05040"
     CDS             5001..6392
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="FGW37_RS04965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05040"
                     /product="YcaO-like family protein"
                     /protein_id="WP_179079942.1"
                     /sec_met_domain="YcaO (E-value: 4.3e-20, bitscore: 71.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPHPDAPGDAPSPDGSGRAQRWRPEVFAPFAEGPGVVFARTAA
                     RSPEFGRTSASGAGRVLIGSAVGDDRADVALRARGELLERMGNVLAGRAAEGAARWVGD
                     HDGWRRRGVPALDPAELGDGTGGTAGRSVRQLWVPARSALTGAELLVPAGEVFLQHRPP
                     PGCGTTSGAGSTGLGARPDRAAAAGHAALELLERDVIRRSWYGPGAPPQRAVPADDLLP
                     GPVRILLTALGLRAALLTLPAPAGTECAVVCVHGGATPGRPGQSFGARCARAGDRAVTV
                     ARAAYEALMVRWSMTSPVALRTWEGWGGRTPPRTALEHALWAFHGQDALGHWTGRRRGR
                     GMTGDALDSGRGDAAGARPGRAGPESRTDGVTDGHGGATRPRPGQEEPGPGPQPSGAVD
                     VPGGRDEGLAALAAHTGGDVLLVDTDTPGIRAEGVHVVRVIAPGARPLPADDSRMPGAL
                     PHPFG"
     gene            6432..7202
                     /locus_tag="FGW37_RS04970"
                     /old_locus_tag="FGW37_05045"
     CDS             6432..7202
                     /EC_number="2.1.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528447.1"
                     /locus_tag="FGW37_RS04970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05045"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_179079943.1"
                     /transl_table=11
                     /translation="MTSSGSPDYTAFAARYDSWFAASGTTAATVSALAGLAEGAGPGPA
                     LELGIGTGRIAVPLAARGVEVHGIDASPAMVEQLRAKPGGEHIPVTMGDFSAGAGADAG
                     IPGAYALVYVVSGTFAELPSQDAQVDCFAGAARRLRPGGLFVLDAHVPEALVADRSTGG
                     QVLPTAGGELVLRFRETDRAAQRYSSHYVVFDKGPGGLGMHRTTVSFRYAAPGELDLMA
                     RLAGLRLRERWGNWTGAPFTSSSAYHVSVYERPE"
     gene            7707..8666
                     /locus_tag="FGW37_RS04975"
                     /old_locus_tag="FGW37_05050"
     CDS             7707..8666
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="FGW37_RS04975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05050"
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_179079944.1"
                     /transl_table=11
                     /translation="MDVEPDLTAAHADQQALQRLLTAVAEDAGLPPLPLHPAAVEAAHR
                     AQAAVRRAGGGAGAGTAPANGWAGPPAPAATGPAAWPVELAGPPDHPHLLRSLRRALAQ
                     LPSRPGADGQPVTAALGPWRTADREALKAAVGLLQGVWPEMLDELRTVVRQVALLDGMA
                     IDGFTDFAVHGAVLINRRRLSATRSGLPGPARLAEALVHEGTHNRCNVAGVQAPFLRPA
                     ADDTAGPLATPLRSDPRPLSGLFQQTVVLARCALLHRRTADEGALPAAGATAVRTRHDR
                     LLTDLGTAVTSLAAHRTGLTEHGAELLRQCAAIARGTA"
     gene            complement(9118..10104)
                     /locus_tag="FGW37_RS04980"
                     /old_locus_tag="FGW37_05055"
     CDS             complement(9118..10104)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /locus_tag="FGW37_RS04980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05055"
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_179079945.1"
                     /sec_met_domain="PhyH (E-value: 2.3e-06, bitscore: 26.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGPDAGPDAGAAVPGPAPGSAPGPVPGAASRPPAGPAALHTVEP
                     GPYGIPVSLRHVLDGALVAVKGGLRHIGALGTIRREAVRAVAAALPAAEASRVSGALEN
                     VHRHADTAQIEAVRTDLETRLRPFSRTVLHDVAGALEPQGARLYLSGHLGIRVMPPQRS
                     VGSGGGELAGLEGFLTPRDAHVDSWFNTAVNSVNLWMAVGPVRSGNGLLFYPDAYRRPL
                     RHDGHTLLPGQRIGAPVDIELEPGDILLFAGDHLHASQPNATDETRFVITKRLCVGVPR
                     YPSRATGWVPYEDPRLWGTRWERWATWRSRLTPGGVRDALRTARGRT"
     gene            complement(10101..11042)
                     /locus_tag="FGW37_RS04985"
                     /old_locus_tag="FGW37_05060"
     CDS             complement(10101..11042)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: TD (4-195). E-value: 1.9e-10. Score:
                     32.3. Matches aSDomain: nrpspksdomains_FGW37_RS04985_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /locus_tag="FGW37_RS04985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FGW37_05060"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_179079946.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 4.4e-08, bitscore:
                     31.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVVTGAAGFIGSHLCAHLLAAGDSVAGIDALTDSYDPALKERN
                     LRGPMSCPAFTFHRADLLDAALDPVLDGADVVYHLAAQPGIRPSWGREFALYADRNVLA
                     TQVLLEAVRGRPLVKFVYASSSSVYGDARAFPTAETACPRPVSPYGVTKLAAEHLCELY
                     RTAYGVPAVALRLFTVYGPRQRPDMAFARLLAAARGGAPFPLYGDGEQTRDFTYVQDVV
                     TGMRDAALSGFTGVANIGGGSPVSMKQAIAAVESVAGPVAVVPRPPGPGDARHTGADIT
                     LAARAFGFRPRTALHDGIAAMAGGRGHRDGAP"
     aSDomain        complement(10458..11030)
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FGW37_RS04985_TD.1"
                     /evalue="1.90E-10"
                     /label="FGW37_RS04985_TD.1"
                     /locus_tag="FGW37_RS04985"
                     /protein_end="195"
                     /protein_start="4"
                     /score="32.3"
                     /tool="antismash"
                     /translation="VTGAAGFIGSHLCAHLLAAGDSVAGIDALTDSYDPALKERNLRGP
                     MSCPAFTFHRADLLDAALDPVLDGADVVYHLAAQPGIRPSWGREFALYADRNVLATQVL
                     LEAVRGRPLVKFVYASSSSVYGDARAFPTAETACPRPVSPYGVTKLAAEHLCELYRTAY
                     GVPAVALRLFTVYGPRQRPDMAFARLLA"
     CDS_motif       complement(10971..11033)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FGW37_RS04985_0001"
                     /evalue="1.90E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="FGW37_RS04985"
                     /protein_end="24"
                     /protein_start="3"
                     /score="10.9"
                     /tool="antismash"
                     /translation="VVTGAAGFIGSHLCAHLLAAG"
ORIGIN
        1 tccatgcccg cgcgcacgcc gagcacgacc acggcaccgg ccccacctgc actgccttca
       61 tcgacacctg gctcgccgcc gacccccacg ccgtgcagcg ccgtcacctg caatggcacg
      121 ccgccctgca gtccattgcc gccggcgact tcgtcgacgc ccgccgtcgt gccgataccg
      181 agctccacca cggggacgtg ggcatgcgct cggccaccaa ctggcgcctg ctgctcgccg
      241 gacagacccc cgcccgcacc gctgggcttg accatatcca ccggctgctg gccgagcccg
      301 gcggctgggc ggaggtcttc cacaccttcc agctcgccct gggcctcgcc gtcgccgccg
      361 acaccgacgc cctgcacgcc ctcgccagcc gcgccgcggg agatccccgc ccggactacg
      421 ccgaggtcct cgcccccgtc gctctggcct tggcccatct caccgcaggc cgccccggac
      481 cggccgtcga cctcctgacc gctctcggcg accggaccga acgcatcggc ggtgtccgtg
      541 tcgaacgtga aatcatccag gacactctcg cgcgcgccct catcgatgcc ggccagccac
      601 agcgtgccgc tcacctgctg caccaccgca ccaccacccg ccggcaccac acctacgaaa
      661 acctcctcct caccacgccg gcccctgccg cgaacacagc caggcgccca gtggtcccgc
      721 ccggtgacgc ggtgccggca cctcgggacc attccggtga ggtctcgtaa ggcccggcgg
      781 agccccggag ccagggagag acacctgcgg gcccaggccg aagcggttct atgcctgtct
      841 cgcccgctgg tccctggtcc ctgatccatg cgtccggctg gacgggacgg ggtacccgga
      901 gtgcggggcc cggtggcggg gctggaccgg cggtcgacag ccgtcttctg ctcgcggcgg
      961 tgcaaagtcc gggcctggcg gaggcggaag gaaccgttca ctagacagtc acagtaaccg
     1021 ggagggtagg gcgtccgccc cgccgcactg attgagaggt ccggcaacag gcaggcccgc
     1081 ctggttttct ccggcggtgc cgggcccgcc cgatgccgtg ccgcgggctt ctgcgccgta
     1141 ccggatggcg tagccgttgc cgatggccct ggtcggtgcc ggaagcgccg ttccggcacc
     1201 tgcgggtggc ggacttcgct gggtcggcac cccgacttgc gcacacgacg aacgccctat
     1261 gtgctccgca cagccatgca tggaaggtac ggcgccttca ttgctaacgg aatcgcggat
     1321 tcctaaaccc ttcccgcgcc gcgtcaccgg tcctgggaca gtttgtacag ctccgcgaag
     1381 agtcctcccc cgacggcgag ttcttcgaag gtgccctcct ccacgatgcg gccctcggcg
     1441 aggacgacga tgcggtcggc gatacgggtg ttggtcagat tgtgcgtgac gaacacggcg
     1501 gcacgtccgg cggcgagctc cttgaagcgg ctgatgacgg tgtgctcggc gcgcgggtcc
     1561 atcgcggagg tgggttcgtc gaggacgagg acggcggcct cgcggtagaa ggcgcgcgcc
     1621 acggcgaagc gctgccactg gccgccggag aggtcgtggc cgcccaggaa cgacttggcg
     1681 aggagggtgt ccaggccgcc cgggaggccg tcgaggacgc tgtcggcgcc cgcggcgcgg
     1741 gcagcggtgc gcaccaggtc atcgccgccg cggggctggc ccagggtgat gttctcgcgg
     1801 gccgccatcg gccagcgcgt gtagtcctgc gggaccagcc cgacgcgcga ccagacgtcc
     1861 tgggggtcgg tctcggcgag gtcggtgccg tcccaggtga cctgcccggc ggtgggcagg
     1921 tagagccccg tcagcagccg ggagagggtg gtcttcccgg agccgttctc cccgaccagc
     1981 gcgacgagtt cgcccgagcg cagggtgaag ctgacacggt ccagggcggg cctgccggac
     2041 ggggcgccgg ggtggcggta ggagacctcg cgggcgcgga tctcccgggg gccgccggcc
     2101 ccgagccgct gcgggccgcg tacggcccgc agctcctcgg ccaggtccag gaaatcctgc
     2161 cagtcctgga ggtagagcga ggtgcggaag agccgggccc ccgccatgac cagggaggtc
     2221 agcgcggccg tggaggcccg tacggccaca accgcggtgg cggcgaccgc caggcccatg
     2281 gcgccgccga ggacgagcgc gccgacagtc agccaggtga gggcgagcat ggtgccggaa
     2341 cccgccgccc cgcagagcgt caccagcagc acgcgggggg cggcgtcgcg gtgccgggtg
     2401 cggatcctgt cggtgaggtg ggcgtaccac tggcgcaggt acggggccat ggtgccggcc
     2461 cggatctcgg cggccgtgcg cggctcggtg ctgaagcggc gcaggaggta gcgcaaccgg
     2521 tcgtcggcga ggctggtgtg ggcggcgcgg tgggcgatgc gggccgcggc gacggcggcg
     2581 gctcccttgg gcagcgcggc gagggccagc agcggcagca gcagggggtg cagggcggtg
     2641 agcacgaccg ccgcggcgat cagctgggcg acggcggaga gcagcacctg ggcgtcggtg
     2701 atcaggtcct gggtggcctg ggcgcccgtg ccggcggcct gcagggcgtc gttgaagtcg
     2761 ggccggtcgt aggccgtcag ctcgacctcc ggggtgtcgg ccaggatgcg ctcgtcggcc
     2821 agggcggtca cctcgggggc cagccgcagg gcggcgtact gggccgtggc gtccgcgcag
     2881 gcgcgcacgg cggcggccgc ggcggcgacg gtgagcgcgg gcagcgccgc gtacaggtgc
     2941 tcggcggccg tgccgggacc gaagagcccg gccatgacgc gcgcggtggc ggcgagcgcg
     3001 accgccgcgg ccgcggccgc cacgcactgg gcgaccagta tctgcaccgt ggccgcccgg
     3061 tccgccgccc accccagccg ggccgcccgg ccgaggacgg cgggcagccg gcccgccatc
     3121 tgccgcaggc tcgcggaggc cacgcggtgg aagtgcgggt cgtcccagcc gagatcgagg
     3181 tcgggctgcg cggcggcggc gggggcggag gcggggatgc tcggggcggc ggcgttcgtc
     3241 accgggtcat ggtgccgtca caccggccgc cgcgtggcgg aactctgccc ccgtggccct
     3301 ttaggggatg caggaccgct tcagcagggc aggaggcccg gcggattcgt cctggtgtac
     3361 gggaaagagt gcggcgcacg ccgcagacgt cgagggccgg tggctcgccg gggcgggggg
     3421 ccaagtacgc gcggacgcgg gcgtactgag cttcgtacgc ccgggccggc cggtgtccca
     3481 cagccgctcg gcggcgcgca gccggccctc ctggtcgcgc acggtcggca cggaccggga
     3541 accgggggtg gagggcgtcc ggttcagcga ggtgcggcag ggcgaagacg ccggccgacc
     3601 cgtcgtggtg gcgcagggcc gggaagcggc ggtcgccggg gcgcagggcg gccgtgtcgc
     3661 ccgcgtgccg aaggggaacg aggcgaggtc gaagcccggc tcctgcagcg cggtggtgag
     3721 ctgaggatcc ccggggcggg cgggccgagc ccgaagacct tggcccgctc cttgcacggg
     3781 agggcctcgg cggcctcgta cacgtccgcc atggccgggg gcggcagcgg cagcggcggc
     3841 agcccgaggg ccgcagtctc gcgccccgcg acggcgggca gctcttcgat gcgggccgcg
     3901 agctgatcgg gcgtcacgtc gtatggcagg cgctcgcggc cgtgccggac cgcctcccgc
     3961 cgcgaccgtg aagtcggggc cgtcgcccca ctgcgccatg gcggccgacg gggcggccgc
     4021 cggacgggga gaagtccccg gggagggtac ggatccggcc ggacccgctc accgcgcggg
     4081 cccggcgggc gtggcgggcc cggcgggcgt ggcgggcggc ccggcggcgc ccttggctgc
     4141 ggggccctcg gccgggaccc tcgcgtagtc gtcggagtgc gggaaggggt cgccgggggc
     4201 ggggacgccg aggaagggcg ccagccgctc gtagccctgc ccggcggtga tatcgaggac
     4261 cagcaggtca ccggggcgtc cggcgaagtg gcggagcacc tcggcggtgt ggcggcgggc
     4321 gaccgtgcgg aaccgctcgg ggtcgaactc gtggctcgcg tagacggcgg cccgcaggaa
     4381 gcgctgcagc tgcggataga cctcgctccg gccggtcttg ccgtcgatcg cccgcttcca
     4441 gtgggtgcgg caggaggcca gccagccctc ctcctcgcgg acggtgagga tgaacttggc
     4501 gcccggccag gcccggtcca ggtcctggta gtacggcacg acggtgatgt cggtgatgcc
     4561 gtcgcggtgg tcgagcagcg ggaagcgggc ggtaccggcg aggagcgccg cgtaggtgcc
     4621 ccgatcggcc gggtagtggg ccacgtcgaa gccgagcgtg cgcagggcga cggtcaggga
     4681 ccgggttccg gtgcgggaca ggccgatgcc gaagatcttc ggccgggcgg cggggagcag
     4741 cggtccgggg cgggggacgg aacccgggtg gggaacggat ccggggcgga ctgcgcggtc
     4801 gggtgtcatg tgttcgcggc ctcctcgcgg gggggggatg gcagggcggt acgaggccct
     4861 gcggcccgtg cgctgtcgcc accgtgccgg atcgcacgca aatgtcaacg gcacgccggg
     4921 cggctggaat tcaccgccgc gacgccggtt gcgccgggtg ccgttgtgcc ggcgctgctc
     4981 ctttggtcca atcgagggcg atgaccacac cgcacccgga cgccccggga gacgcgccgt
     5041 ccccggacgg ctccggacgt gcgcagcgct ggcggcccga agtgttcgcc ccgttcgccg
     5101 agggcccggg cgtggtcttc gcgcgcacgg ccgcgcggtc accggagttc ggccggacga
     5161 gcgcgagcgg cgcgggccgg gtcctcatcg gcagcgccgt cggcgacgac cgcgccgacg
     5221 tcgcgctgcg cgcccggggc gagttgctgg agcgtatggg caacgtgctg gccggacggg
     5281 ccgccgaggg cgccgcgcgc tgggtgggcg accacgacgg ctggcggcgg cgcggcgtgc
     5341 ccgccctgga cccggccgag ctcggcgacg gcacgggcgg gacggccggc cggtcggtgc
     5401 gccagctgtg ggttccggcc cgctccgcgc tcaccggcgc cgaactgctc gtcccggcgg
     5461 gcgaggtgtt cctccagcac cgcccgccgc ccggctgcgg cacgacgtcg ggagcgggat
     5521 cgacgggcct gggagcccgg ccggaccggg cggccgcggc cggtcacgcc gccctcgaac
     5581 tcctcgaacg ggacgtgatc cggcgcagct ggtacgggcc cggggcacca ccgcagcgcg
     5641 ccgtccccgc ggacgacctg ctgcccgggc ccgtacggat cctcctcacc gccctgggtc
     5701 tgcgggccgc gctcctgacg ctgcccgccc cggccggcac ggagtgcgcc gtggtctgcg
     5761 tccacggcgg ggcgacgccc ggccgccccg gccagagctt cggcgcccgc tgcgcgcggg
     5821 cgggcgaccg cgcggtgacg gtggccagag ccgcctacga ggccctgatg gtgcgctgga
     5881 gcatgaccag ccccgtggcc ctgcgcacgt gggaaggctg gggcggccgg accccacccc
     5941 gcacggccct ggaacacgcc ctgtgggcct tccacgggca ggacgccctg gggcactgga
     6001 cggggcggag gcgggggcgg ggcatgacgg gggatgcgct cgacagcggg cggggcgatg
     6061 cggccggggc gcggccggga cgggccgggc cggagagccg gacggacggg gtgacggatg
     6121 ggcacggcgg cgccacccgg ccccggccgg gtcaggagga gccggggccg gggccgcaac
     6181 caagcggcgc cgtggatgtg cccggcggca gggacgaggg gcttgccgcg ctcgccgcgc
     6241 acaccggcgg cgacgtgctg ctcgtcgaca ccgacacccc cggcatccgc gcggaaggcg
     6301 tccacgtggt gcgggtgatc gcccccggtg cccggcccct gcccgccgac gacagccgga
     6361 tgcccggcgc gctcccccat cccttcggct gaccgcccac cggccggccg accgcgagaa
     6421 agggctcgac catgacctca tccggctcgc cggactacac cgctttcgcc gcccgctacg
     6481 actcctggtt cgccgcatcc ggcaccaccg cggccaccgt gtcggccctc gccgggctcg
     6541 ccgagggggc cgggccgggg ccggccctgg agctgggcat cggcaccggc cggatcgcgg
     6601 tgccgctcgc ggcgcgcggc gtggaggtcc acggcatcga cgcctccccg gccatggtgg
     6661 agcagctgcg ggccaagccc ggcggcgagc acatcccggt gacgatgggg gacttcagcg
     6721 cgggagcggg agccgacgcc ggcatcccgg gggcgtacgc gctcgtctac gtcgtcagcg
     6781 gcacgttcgc cgaactgccc agccaggacg cccaggtgga ctgcttcgcg ggcgcggccc
     6841 gacggctgcg ccccggcggc ctgttcgtcc tggacgcgca cgtaccggag gccctcgtgg
     6901 cggaccgctc cacgggcggc caggtgctgc cgaccgcggg cggcgagctc gtcctgcggt
     6961 tccgggagac cgaccgggcg gcgcagcgct acagctccca ctacgtcgtc ttcgacaagg
     7021 gcccgggcgg cctcggcatg caccgtacga cggtgtcgtt ccggtacgcg gcgccggggg
     7081 agctggacct gatggcgcgc ctcgcggggc tgcggctgcg cgagcgctgg ggcaactgga
     7141 cgggcgcgcc gttcacgtcg tccagcgcgt accacgtctc cgtctacgaa cgcccggagt
     7201 gacccgaccc gggggaaagg agggcggcat gggcgcgcgt gaccggcgtc ggcttctccg
     7261 ccgaggggac ggccacggcg cgctccctgg ttttgagcca cggcgaccgc ggatacgcgg
     7321 aaaccgctgg ccggtgggcg ggtgcgggtg cctcggaacg gcccgttcca ccatggcgat
     7381 catgctgccc tgtgggcacc atggcatccg ggtgcgccgg tattccggcg cctcgtcgaa
     7441 cacaagacgc ccagacccct caccgccgaa ggagcggaaa tgccccagga catcgtagaa
     7501 gtccaggaag tcccggccga ggaactcccc gagctcgccc tcatgatcga cataccgttc
     7561 gaggagtaac ccggccgcgc tgcgcggacc gcactccggc ggccgaccgg gcacgggccc
     7621 ggtcggccgc tccgtgcgac ccgagggcga gggcaggggc aagggcaggg gcaagggtgg
     7681 ctacgggggc gaggcgggag gactgcgtgg acgtcgaacc ggacctcacg gcggcgcacg
     7741 ccgaccagca ggcgctgcag cggctgctga cggcggtggc ggaggacgcg ggactgccgc
     7801 cgctccccct gcacccggcc gccgtggagg ccgcgcaccg ggcgcaggcc gcggtgcggc
     7861 gcgcgggcgg gggcgcgggt gcgggcaccg cgccggcgaa cgggtgggcc gggccgcccg
     7921 cccctgccgc gaccgggccc gccgcgtggc cggtcgagct cgccggcccg ccggaccacc
     7981 cccacctgct gcgcagcctg cggcgcgccc tggcccagct gccgtccagg ccgggggccg
     8041 acgggcagcc ggtcacggcc gcgctcggcc cgtggcggac cgccgaccgg gaggccctga
     8101 aggcggcggt agggctgctt cagggcgtgt ggcccgagat gctggacgaa ctccgtaccg
     8161 tggtccgcca agtggcgctc ctggacggca tggcgatcga cggattcacc gacttcgccg
     8221 tccacggcgc ggtcctgatc aaccgcaggc ggctctcggc gacccgctcc gggctgcccg
     8281 gcccggcccg gctggccgag gcgctggtcc acgagggcac gcacaaccgc tgcaacgtgg
     8341 cgggagtaca ggcgccgttc ctgcgccccg cggccgacga cacggcgggc cctctggcca
     8401 cgcccctgcg gtccgatccg cgcccgctca gcgggctgtt ccagcagacg gtggtcctgg
     8461 cccggtgcgc cctcctccac cggcggacgg cggacgaggg tgcgctccct gccgctgggg
     8521 cgacggcggt acgcacccgc cacgaccgcc tgctgacgga cctgggaacg gcggtaacgt
     8581 ccctcgccgc gcacaggacg ggcctgacgg agcacggggc ggagctgctg cggcagtgcg
     8641 cggcgatcgc gcggggcacc gcgtagggcc gtccggcccg gcagcgccgg actactcgtg
     8701 cccacgtccc accgtcgcgg cgcgaacaga tgcctgcggc accacacgcc cggcagcaac
     8761 aaccactgcc caccacaagc cgggaccaac ggcaccccac cccgccgacg gcgatcgcac
     8821 agacgccgcg tagggccgtc cggcccggca gcgccggact actcgtgccc acgtcccacc
     8881 gtcgcggcgc gaacagatgc ctacgacacc gcacgcccgg cagcaacagc cgctacccac
     8941 cacaggccag gacccacgca agcccggccg acccggcagc gccgaactgc ttgtgtccac
     9001 atctcgccgt cgccgcgcga acagatgcct gcggcagcgg agtacgggcc cccgcggcag
     9061 cacacacccg tgcacgactt cccgcgcgcc ggaccggcgg ggcccggtcg tctcccccta
     9121 cgtccgcccc cgcgccgtcc tcagcgcgtc tctcacgccc cccggtgtca ggcgtgaccg
     9181 ccacgtcgcc cagcgttccc agcgtgtccc ccagagacgg gggtcctcgt acgggaccca
     9241 gcccgtcgct cgcgacgggt agcgcggtac ccctacgcac agccgcttcg tgatgacgaa
     9301 gcgggtctcg tccgtggcgt tgggctggga cgcgtgcagg tggtcgccgg cgaagaggag
     9361 gatgtccccg ggctccagtt cgatgtcgac cggggcgccg atccgctggc cgggcagcag
     9421 tgtgtgtccg tcgtggcgga gggggcgccg gtacgcgtcg gggtagaaca gcagaccgtt
     9481 gccgctgcgg accgggccga cggccatcca gaggttgacg gagttgacgg cggtgttgaa
     9541 ccaggagtcg acgtgcgcgt cacgcggggt caggaagccc tcgagtccgg ccagttcccc
     9601 tcccccggac ccaacggagc gctgcggcgg catgacgcgg atcccgaggt gcccgctgag
     9661 atagagccgg gctccttgcg gctccagggc cccggccacg tcgtgcagga cggtgcggga
     9721 gaagggccgc agccgtgtct ccaggtcggt gcggacggcc tcgatctgcg cggtgtccgc
     9781 gtggcggtgc acgttttcca gcgccccgga gacccgggac gcctcggccg ccggcagggc
     9841 ggcggccacg gcgcggacgg cctcccgccg gatcgtcccc agcgcgccga tgtggcgcaa
     9901 tccgcccttg acggccacga gggcgccgtc gaggacgtgc cgcagtgaga cggggatgcc
     9961 gtacgggccg ggttcgacgg tgtgcagggc ggcgggcccg gccgggggcc gggaggccgc
    10021 acccgggacc ggcccgggag cagatccggg agcaggcccg ggaaccgccg ccccggcgtc
    10081 gggcccggcg tccggccccg tcacggcgcc ccgtcccggt gcccccggcc gccggccatg
    10141 gccgcgatcc cgtcgtgcag cgccgtccgc gggcggaagc cgaaggcgcg ggcggcgagg
    10201 gtgatgtcgg ccccggtgtg ccgggcgtcg cccggccccg gtggccgcgg gaccacggcg
    10261 acgggcccgg cgacgctttc cacggcggcg atggcctgtt tcatggacac cggcgagccg
    10321 ccgccgatgt tggccactcc ggtgaagccg gacagggcgg cgtcccgcat gccggtgacg
    10381 acgtcctgga cgtaggtgaa gtcgcgggtc tgctcgccgt ccccgtagag cgggaacggc
    10441 gccccgcccc gcgccgcggc caggaggcgg gcgaaggcca tgtcgggccg ctgccggggc
    10501 ccgtagacgg tgaacagccg cagggcgacg gcgggcaccc cgtaggccgt gcggtacagc
    10561 tcgcacaggt gctcggccgc gagcttggtc accccgtagg gcgagacggg ccgggggcac
    10621 gccgtctcgg ccgtcgggaa ggcacgggcg tcaccgtaga cggaggagct ggacgcgtag
    10681 acgaacttga ccagcgggcg cccccggacg gcttccagca gcacctgggt ggccaggacg
    10741 ttccggtcgg cgtagagggc gaactcgcgc ccccaggaag gccggatgcc gggctgggcg
    10801 gcgaggtggt agacgacgtc cgcgccgtcg agcacggggt cgagcgcggc gtccagcagg
    10861 tcggcgcggt ggaaggtgaa ggccgggcag ctcatcggac cgcgcaggtt gcgctctttg
    10921 agggccgggt cgtaggaatc ggtgagcgcg tcgatgccgg cgacgctgtc gccggcggcc
    10981 aggaggtgcg cacagaggtg ggagcctatg aagccggccg cgccggtgac cacggcgcgc
    11041 actacggacg cccttcccgg ggcaggtccg cgggttcccc ggggacccgt ggtgccgccc
    11101 gggccccggg cggcaggaca cggtcggcgc gcccccgcca cgtgtgacgc tccagcagct
    11161 gccgccgggc cgcctccccg agggcctggc gccgcgccgg gtcggcggcc agggccatga
    11221 cggcgtccgc ccacgccgcg acgtcgccgg gcgggcacat caggcaggtg tgcccgtcgg
    11281 ccatgacctc ccggagcacc gggaggtcgg agatgacgat cgccttgccg tacgacatgt
    11341 actcgaagag cttcatcggg gagacccagc gggcgatgtc tccgatgccc cc
//
