LOCUS       NZ_CP040274            20717 bp    DNA     circular CON 31-AUG-2024
DEFINITION  Nostoc sp. C052 plasmid pC052_B, complete sequence.
ACCESSION   NZ_CP040274
VERSION     NZ_CP040274.1
KEYWORDS    RefSeq.
SOURCE      Nostoc sp. C052
  ORGANISM  Nostoc sp. C052
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Nostoc.
REFERENCE   1  (bases 1 to 336425)
  AUTHORS   Li,F.-W.
  TITLE     Complete genomes of symbiotic cyanobacteria from bryophytes provide
            new insights into the evolution of Vanadium-type nitrogenase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 336425)
  AUTHORS   Li,F.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2019) Boyce Thompson Institute, Boyce Thompson
            Institute, 533 Tower Rd, Ithaca, NY 14853, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013393905.1-RS_2024_08_31
            Annotation Date                   :: 08/31/2024 08:18:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,527
            CDSs (total)                      :: 8,409
            Genes (coding)                    :: 8,174
            CDSs (with protein)               :: 8,174
            Genes (RNA)                       :: 118
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 84 of 235
            Pseudo Genes (incomplete)         :: 168 of 235
            Pseudo Genes (internal stop)      :: 38 of 235
            Pseudo Genes (multiple problems)  :: 50 of 235
            CRISPR Arrays                     :: 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:15:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 51883
            Orig. end                         :: 72600
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP040274.1.
            Bacteria and source DNA available from Fay-Wei Li, Boyce Thompson
            Institute, 533 Tower Rd., Ithaca, NY 14853, USA.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20717
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="proteusin"
                     /region_number="2"
                     /rules="(PoyD or TIGR03793)"
                     /tool="antismash"
     cand_cluster    1..20717
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PoyD or TIGR03793)"
                     /kind="single"
                     /product="proteusin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20717
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10717](-)"
                     /cutoff="20000"
                     /detection_rule="(PoyD or TIGR03793)"
                     /neighbourhood="10000"
                     /product="proteusin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10717)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PoyD or TIGR03793)"
                     /neighbourhood="10000"
                     /product="proteusin"
                     /protocluster_number="1"
     misc_feature    970..985
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.48"
                     /tool="antismash"
     misc_feature    1096..1111
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.16"
                     /tool="antismash"
     misc_feature    1729..1739
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    2685..2701
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     misc_feature    3164..3177
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.85"
                     /tool="antismash"
     gene            4072..4224
                     /locus_tag="FD723_RS34900"
                     /old_locus_tag="FD723_34775"
     CDS             4072..4224
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013561.5"
                     /locus_tag="FD723_RS34900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34775"
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_218651870.1"
                     /transl_table=11
                     /translation="MARKNAANLTIQLSLEEKIILEEYCDKMQRTKSDVIREMVRSLKK
                     YLEGS"
     gene            4558..4983
                     /locus_tag="FD723_RS34905"
                     /old_locus_tag="FD723_34780"
     CDS             4558..4983
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FD723_RS34905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FD723_34780"
                     /product="hypothetical protein"
                     /protein_id="WP_179069830.1"
                     /transl_table=11
                     /translation="MQNTKELKFILSELTYELLETFNLPTKWINQLKLLPTLTAFTETE
                     FNELVDQYLIKLGSVSRNRIYEAAAITFYHQQSAIPVIPILLCDDAPQFKLITDNLALC
                     WVHEGRHYKKLSPFIASHQKILDDFLSRFWKYYRKLY"
     gene            4996..5169
                     /locus_tag="FD723_RS34910"
                     /old_locus_tag="FD723_34785"
     CDS             4996..5169
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025469.5"
                     /locus_tag="FD723_RS34910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34785"
                     /product="DUF4277 domain-containing protein"
                     /protein_id="WP_256875336.1"
                     /transl_table=11
                     /translation="MDYQKEEFESKKLDHLGIVTGIIDDIVIVEKIKNIFLIDSRENIN
                     TGEVVKAIISYM"
     gene            5514..6437
                     /locus_tag="FD723_RS34915"
                     /old_locus_tag="FD723_34790"
     CDS             5514..6437
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1242: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /locus_tag="FD723_RS34915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34790"
                     /product="IS110 family transposase"
                     /protein_id="WP_256875356.1"
                     /transl_table=11
                     /translation="MRTFTTMTQDLLKLSDWLTSHDCTHVAMESTGEYWRPVFNILEGN
                     FEVMLVNARHIKAVPGRKTDIKDSQWIAELLQHGLLRASFIPPVEQRDLRDLTRHRSNF
                     IRERVNLVNRVQKVLEAANIKLASVASDVMGVSGRAMLAAIVEGSASPELMADLAKGTM
                     RKKHDLLIQALEGRVRPHQRFILAHLLCQIDSIDETIKCFDQQIEEYCCPFEQAVELID
                     TIPGIARRTAEIIVSEIGIDMSRFPSAQHFAAWAGVAPGNYESAGKKLSTSTRKGNRSL
                     RTILVQAAHALAHTKTYLADLKILLR"
     gene            complement(6667..7383)
                     /locus_tag="FD723_RS34920"
                     /old_locus_tag="FD723_34795"
     CDS             complement(6667..7383)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (6-179). E-value: 4.5e-13. Score:
                     41.4. Matches aSDomain:
                     nrpspksdomains_FD723_RS34920_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154214.1"
                     /locus_tag="FD723_RS34920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34795"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_179069831.1"
                     /sec_met_domain="adh_short (E-value: 4.6e-32, bitscore:
                     105.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLQNKVAIITGASGGIGEAVAKELDAAGMNLVLTARSQEKLERL
                     ASNLQHAKVIPGEITNPELPQKLVDFAIQTFGQLDVVFNNAGVMTVGPIEGVDIEAICQ
                     MVRVNVEAAYRIGYTALRHFKESGSGFLLNTSSIAGLKTVPQLAAYCGTKFAIEAFTDS
                     LRVELAGTDIRVSSIAPGTVDTGLYDKWGEEQKNFVYSGGALQSADIARCVRFILEQPG
                     NVLIPRLLVVPAAEPV"
     aSDomain        complement(6847..7365)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FD723_RS34920_PKS_KR.1"
                     /evalue="4.50E-13"
                     /label="FD723_RS34920_PKS_KR.1"
                     /locus_tag="FD723_RS34920"
                     /protein_end="179"
                     /protein_start="6"
                     /score="41.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAIITGASGGIGEAVAKELDAAGMNLVLTARSQEKLERLASNLQH
                     AKVIPGEITNPELPQKLVDFAIQTFGQLDVVFNNAGVMTVGPIEGVDIEAICQMVRVNV
                     EAAYRIGYTALRHFKESGSGFLLNTSSIAGLKTVPQLAAYCGTKFAIEAFTDSLRVELA
                     GTDIRVSSIA"
     CDS_motif       complement(7270..7359)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FD723_RS34920_0001"
                     /evalue="2.40E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="FD723_RS34920"
                     /protein_end="38"
                     /protein_start="8"
                     /score="17.0"
                     /tool="antismash"
                     /translation="IITGASGGIGEAVAKELDAAGMNLVLTARS"
     misc_feature    7373..7386
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.86"
                     /tool="antismash"
     gene            complement(7656..>7742)
                     /locus_tag="FD723_RS34925"
                     /old_locus_tag="FD723_34800"
                     /pseudo=""
     CDS             complement(7656..>7742)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611877.1"
                     /locus_tag="FD723_RS34925"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34800"
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="RAIATRYDKLKFNFLGAIYLAATVIWLN"
     gene            complement(8062..9663)
                     /locus_tag="FD723_RS34930"
                     /old_locus_tag="FD723_34805"
     CDS             complement(8062..9663)
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873533.1"
                     /locus_tag="FD723_RS34930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34805"
                     /product="GMC oxidoreductase"
                     /protein_id="WP_179069832.1"
                     /transl_table=11
                     /translation="MFDKKFHRRHFIQSAALFSASLAASVASPIRTRASDDYIDALVIG
                     SGFGGAIAALRLGQAGVNTLILERGKRWPITASQNTFATFNNPDGRASWLNSTSVFGDT
                     VDIYTGVFETLQEKGIIVVSGAGVGGGSLVYNAITYKPKREIFYKVFDRATVDYDELDS
                     VYYPRVQQMLKSSPIPQDVLATTYYGKTRFFLENADKAGLPSFLLNLAVNWDTVRQEIN
                     GSKVPSVIDGQCWWGINSGAKNSLDQNYLPQAENTGHVDILPLHVVTDIAEIRGEKKYR
                     VFCNEINESGQIIRKKTFTCRYLFVAAGSVGTSKLLVKAREKGTLPYLNNSVGKDWGTN
                     GDNITIRSDLGAVTGNGGTAGAVIEQLDGPTPIVVESLELGNNADGIQSCLGLGIPRPA
                     GKFNYDPSTDTVNLNWPTSVDDRIVKKTEKLFSTLDNSINNFGRRPRHKIKEGMISTSP
                     IATSQNNDAAITGHPLGGVPIGKACDPFGRVYGYNRLYVVDGSLIPGSAACTNPSFTIA
                     ALAERCLDKIIAQDIK"
     gene            complement(10001..10717)
                     /locus_tag="FD723_RS34935"
                     /old_locus_tag="FD723_34810"
     CDS             complement(10001..10717)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     proteusin: TIGR03793"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03793.1"
                     /locus_tag="FD723_RS34935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34810"
                     /product="NHLP leader peptide family RiPP precursor"
                     /protein_id="WP_179069833.1"
                     /sec_met_domain="TIGR03793 (E-value: 1.8e-18, bitscore:
                     60.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTTNAPISDTSTNFPEVRKLWSGVDITNFGPYYEALQTRLIDRI
                     WSDEALKQEILTNPRTVFERETGITFPADTEVKVLEEPENTFYFVLPATPPVEEHWYRY
                     EQFSSWWPIIDTFYYFYNRLGGTTKARAYRECLQALWIVRSWTNETFRQQLLSDPRSIF
                     EGEIGIPFPAELKIQSLEETSNLIYFILPKNPQFEKFDSSATVGEWWKASHGWWWWCTS
                     LRFRQPAMNTVTGVVG"
     misc_feature    10894..10907
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.39"
                     /tool="antismash"
     gene            11330..12835
                     /gene="malQ"
                     /locus_tag="FD723_RS34940"
                     /old_locus_tag="FD723_34815"
     CDS             11330..12835
                     /EC_number="2.4.1.25"
                     /GO_function="GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="malQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012412055.1"
                     /locus_tag="FD723_RS34940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34815"
                     /product="4-alpha-glucanotransferase"
                     /protein_id="WP_179069834.1"
                     /transl_table=11
                     /translation="MPFPRSSGILLHPTSFPSQFGIGDFGSEAYRFVDFLVESKQQLWQ
                     ILPLGPTGDTNSPYASYSAIAGNPLLISPELLYNKGLLTEDFTNLPKFSTERIDFSQVI
                     QAKIPILQKACKNFKISAKSEQEREFKEFCQSQAYWLDDYALFMALKDAHNGASWNTWE
                     MAIAQRKPEAIEQWEQRLADEVFYHKYLQFEFFSQWSQLKRYANERGIQILGDIAIYVA
                     HESADVWANPGNFCLDEQTGEPALMAGTPPDYFSTTGQLWGNPVYNWVRLQQENFKWWV
                     QRFQSMLNNVDLIRIDHFRGFQAYWAVKQGETTAMNGTWLEAPGEAFFEVLKQKLGNLP
                     IIAEDLGEITPEVYALRDRFQFPGMKILQFAFGDASQAEKRFLPFNYQSNCVVYTGTHD
                     NDTTVGWFNQLLPPAREQLLSYLGCQSESGIHWDLIRLALSSVANQAIIPLQDVLGLDT
                     GARMNFPGKTEGSWGWRYQSTALTPEISDRLGTMTEIYGRVSE"
     gene            13285..14679
                     /locus_tag="FD723_RS34945"
                     /old_locus_tag="FD723_34820"
     CDS             13285..14679
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1242: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006509772.1"
                     /locus_tag="FD723_RS34945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34820"
                     /product="IS110 family transposase"
                     /protein_id="WP_179069835.1"
                     /transl_table=11
                     /translation="MKLPPNQGHNQPVLTEKVSSTLEEINQNAAGIDLGGGEHWVCVPA
                     DRTDKNVRRFGCFTPDLIAMADWLIECRVDTVAMEATGVYWIPVFQILEAKGFEVKLVN
                     AHHVKTVPGRKTDVLDCQWLQKLHTYGLLSGSFRPQDQVCVLRSYIRQRDSLIKSASTH
                     VQRMQKALIQMNLHLHKVISDVTGLTGMKIIKAIVAGEQDPQVLATLKDPRIKSSTADI
                     AKALTGDYRREHLFVLQQELTLYEVYQQQISAIDAEIEKCLASFQPQTLDEPPTTPKKR
                     RKKPTANHPNFDLHKYLYRMAGVDFTLINGLDALTVQTILSEVGLDPKRFPTVKHFTSW
                     LGLCPGQKVTGGKVKSSKTRTVVNRAANAFRMAAFSLTHSRSALGGFYRRLRSRLGAPK
                     AITATAHKLARLFYRMWITAGQYSDPGMDYYEQKYQELILKHLQQKAQAFGLELIPISD
                     STQCVS"
     misc_feature    complement(15053..15066)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.43"
                     /tool="antismash"
     gene            15234..15917
                     /locus_tag="FD723_RS34950"
                     /old_locus_tag="FD723_34825"
     CDS             15234..15917
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154221.1"
                     /locus_tag="FD723_RS34950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34825"
                     /product="DJ-1/PfpI family protein"
                     /protein_id="WP_179069836.1"
                     /transl_table=11
                     /translation="MIPRSLGGKKIAILLESEFIPEEIEAYQKRFSELQATVHLMSRLW
                     NQPSVRFFSDEDTGNTPKTIDVNIDFQNVDVNDYAAVIMSANYTSVRLRYFEPPTGQPI
                     SAEQVRTSPAVQFYASAMVNPRIIKGALCHGLWIVTPIPELLKGRQVICHEVVLADIIN
                     AGAIYTPSPSGVVVDGDLVTGRSKHEVEPFIDAITEQIQQLSIATNRFSGRRATTSVSK
                     LKVAS"
     gene            16073..16900
                     /locus_tag="FD723_RS34955"
                     /old_locus_tag="FD723_34830"
     CDS             16073..16900
                     /GO_function="GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154220.1"
                     /locus_tag="FD723_RS34955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34830"
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="WP_179069837.1"
                     /transl_table=11
                     /translation="MKKILIILSEYGYWGEELVGPLETFDAAGYQVDFATPTGKRPVAL
                     PPSMDPTYVDPPLGRPVVSQEMAEKVKRLEDPKNPRLDNPIILSQWLPDRPYWSAEKFL
                     REMETYNNALDEIQPDLDQYDALLIVGGSGPIVDLVNNHRVHDLILSFYKKGKAIAAEC
                     YGVTCLAFARDLADRKSIIQGKHVTGHCKEYDYKDGTGFVGADINMGPPPYPLEYILHD
                     ATAPDGAYHGNFGKEISVIVDYPFITGRSTADSYTTGQKLVEVLDKGLRRYGW"
     misc_feature    complement(16903..16918)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.44"
                     /tool="antismash"
     gene            16933..18642
                     /locus_tag="FD723_RS34960"
                     /old_locus_tag="FD723_34835"
     CDS             16933..18642
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154219.1"
                     /locus_tag="FD723_RS34960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34835"
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="WP_179070045.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.3e-47, bitscore:
                     155.9, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 8.4e-35, bitscore:
                     114.1, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANRNGRFAIIEQLLADGIEYMFGNPGTVEQGFLDALKDYYPEFK
                     YIFALQETIAVGAGDGYARATKKPTIVQLHSGVGLGNGIGMIYQAMRGHAPLVVLAGES
                     GICYDAMDAQMAADLVSMAKPVTKWSTRVVDPSSLLRVLRRAIKIAATPPMGPVFVSLP
                     MDILDAVNYEEVVATSFPVTRVAPEPEQLETAANLLAAAKHPLIIMGDGVAFSDAQAEL
                     TSVAELIGGQVWGADSSEANMSASHPLFGGLLGHMFGDDSRRVTSQADVVLIVGTYVFP
                     EVFPALSGVFAPSAKVIHIDLNTYEIAKNFPVDLGLLGDPKTSLSKLAIALESTLTPEQ
                     KKEVFVRASQIAETKKQESVARLEADKAVRDAVPLHMTRFAEELAAHLPADAIIFDEAI
                     TNSPELCRYLPPTKLGHYFQTRGGSLGVGIPGAIGIKLANPDKTVFAFTGDGGSMYTIQ
                     ALWTAAHHNIDAKFVICNNHSYQILKLNILHYWGEQQIPEHDYPSSFNLCNPSIDFAEL
                     AKSLGVQGIRVETPDQIAPAIQKALDHNGPFLIDLVLTNEIVGTKIGVKCGQ"
     gene            18682..19131
                     /locus_tag="FD723_RS34965"
                     /old_locus_tag="FD723_34840"
     CDS             18682..19131
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154218.1"
                     /locus_tag="FD723_RS34965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34840"
                     /product="hypothetical protein"
                     /protein_id="WP_094346164.1"
                     /transl_table=11
                     /translation="MTVAKLAPLTELEIKQLAADWYLKLDVHAPLEDYIPFLAEDGLEM
                     RFPEATVYGFDGFKGWYDRVIGIFFDEVHTLKEVNVTPSGDEASVQVIVKWEASVWNPP
                     AAKSQRIVLDAYQTWIVKRSPNTGKPVIATYIVDSLSYYEGSARL"
     gene            19218..19616
                     /locus_tag="FD723_RS34970"
                     /old_locus_tag="FD723_34845"
     CDS             19218..19616
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024092.5"
                     /locus_tag="FD723_RS34970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FD723_34845"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_094346163.1"
                     /transl_table=11
                     /translation="MKITEDKKMTVSTISLGRKFFDKHIETISAGKIDEMVDRDYAEDA
                     VLITFFNGFENTPAPITVNGRENIKTFFHDYLRTIEYIDIKSLDFTEAEDAIFFQAKFN
                     CKLGQVSVGDAWTLRNGQIAYHFGFWVS"
     gene            19823..20644
                     /locus_tag="FD723_RS43455"
     CDS             19823..20644
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024092.5"
                     /locus_tag="FD723_RS43455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_256875337.1"
                     /transl_table=11
                     /translation="MSTPIEQLLVSPISKLYKEHIGFIKAKNVDGLLNQYADDALLIST
                     LTEDRQPLYVQGRQALKEFFEGRIFGLEDFEIRLNQWAETENTLMMVEELKTASQTGEV
                     GSVEFYDNWFLQDGKIAIHFAGVIQYPDKTYVNAGTVKSEPPDSPLGKFYKEHIGFIKA
                     KNVDGLLNQYADDVLLISTLTENRKPLYVRGRQALKQFFESRIFSLEDLEVKLDQWAET
                     ENTLMIVESLKTRSVNGDVGEVSFYDNWVLRDRKIAVHFAGVVQYPDGTYA"
ORIGIN
        1 gggctaaaag ctactgacca gacgtaggcc gaatgtccac gtaagatttt tttgcactca
       61 ccagtactga catcccacaa ctttattgta tgatcgggac tgctactggc tagggtctga
      121 ccatcttcac taaaggcgac tgttttaacg atagaattat tctcaccaac ctgaaaaata
      181 ttgaaacaag tactattttt gacactccac aaccgaattg ttccgtcata accactgctt
      241 gccagcagtt caccatctgg actaaaagca attgaccaaa cccaattttt atgtcctttt
      301 agggtttctt tgcactcacc agtactaaca tcccacaact ttattgtgtg atcccatcca
      361 ccacttgcca ctgtcctccc atctggactg aaggcgactg atgaaaccgc agcatgatgt
      421 cccaggaacg tttgtaaggc ttgacccgta ttcacatccc atagcttcac ggtttgatcg
      481 tcactaccgc ttaccaacat ttgcccacct tgactgaagg cgactgacgt aaccgcagca
      541 cgatgtccca ggaacgtttg caaggcttga cccgtattca cattccatag cttcatcgtt
      601 tgatcgtcac tgccgcttat caacatttgc ccatctcgac tgaaggcaac cgatcgaact
      661 gcaccacaat gtctcaggaa agtttgcaag gctttacccg tattaacatc ccatagtctc
      721 actgcgcggt cttgactgcc acttgccagc gtccttctat ctgaactaaa ggcaactgac
      781 caggaattgc tagtataccc aatgaaagtt ttaagacatt gaccagtgct aacgttccaa
      841 acttttatag tttggtcatc actgctactg gcaagaagat cgccttgcaa attaaaggca
      901 acagaaagca cccaaccaga atgtccttca agagttctaa gacattgacc agtgctaatg
      961 ttccatagcc tcaccgtctg gtcgagacta ccactagcga tgaaattgcc ttgaggacta
     1021 attgcagatg accaaactgg agcacgatgc ccatgtagac ttttgataca ttcaccagtg
     1081 tttaagtccc ataatttcac tgtgtggtca tcgctactac tcactagtat ttggccgtct
     1141 ggactgaaag tgattgaccg taccccatca tcatgtccat gaaaaattct tttgcattca
     1201 ccagtaccaa tatcccacaa cctgactgtg ttgtcatcgc taccgctggc tagggtctgt
     1261 ccgtctggac taaaggctac tgatattacc caatttgtat gtcccgaaaa agttctaagg
     1321 cattcaccag tgctaatact ccatagcctt attgtgcgat cgttactggc actgactaac
     1381 gtgttaccat ccggactaaa ggctactgac caaatctcat tactatgttt ttgaaacgtt
     1441 ttcagacatt gccctctact aatatcccac aggcgcacgg tatagtcact gcttccacta
     1501 gcaaggatac taccatccgg actaaaggct agtgacggaa tccaattagt gtgcccctta
     1561 caagttagaa ggtgcttcca atccgaaact tggtaaaagt ggatctcacc accagtatca
     1621 cccattgcca aaagttttcc atcagggcta aaggctaccg acgaaacacc accaaaggtt
     1681 tcagcaaaaa cagacctatc aaaattggcg ttttgaaaat tgacatagtg caatttcaca
     1741 ttccgcaggt ctgcttgcca aacacttaga aaagaaaaat cataaccgct gaggtcagtt
     1801 cctaaataac aaagcagatt gagaatattc ccagttgtat aactgtgttc tagcggtgat
     1861 gtttctcgtg actttcctat aattttagtc agctgatttt caaggtttct tttgcttctc
     1921 aagacagcca gcaacccatc tataactggc ttcagaatca agcgaatttg agtttccctc
     1981 acataatctt tggctgtcgc ctttattaag gcatgacacc taaacaaatc aagattctga
     2041 gtttcaatct cctgacaaac tatctctatc aatcgctgag taacatactc catgactaca
     2101 ggttgtagtg tgaaaagtgc tgtgcttttc tctaccaacg atcgccttac cagagactcc
     2161 agagcctcca gtaaattttg tggtggaatt ggtgatacaa catcgtctcg tatttctgat
     2221 agcgaaactg gctcacgatt gattgctagc cagtacatga tatccttctc taaatttgcc
     2281 aagcgcgaaa actgctggtc tagcagatcg cgaatgctgc caaaaacagc cgtgtaatgt
     2341 tggagaaatt cagcaacatt accatcaaac acatcttgta tcgttgtcgc cactatcttc
     2401 aaagctaggg cattacctgc ataacgctcg actactgctg gtaattctga ctctgagcca
     2461 gaaaaaccct tggcttcaaa gattttttgc cctgctacta cctctaaacc actgagttgt
     2521 aatgagcgaa cagccagtgt ttctcctcca agtgaggcta catctttggg tttttcacga
     2581 gaagtcagca acaaggtgct ttgatgcatg acttgtccca atcgttttac cagtaagccg
     2641 taatcttcat agccttctcg atagcatcct gcataagcgc ctgtttgcaa aatagtctca
     2701 acattatcta atacgatcag acagggctgt tgttgcaaat attcaattaa ttgtgatatt
     2761 ctactatcta cactttttgg taagtcaatt tctaaaacct gctcattaga gaaaaattga
     2821 attaagttag ccagcaggtc aaaaagtggg ggagcattgt agagtgatcg ccaaataaca
     2881 tactcaaacc atccttgaac tttttgtgct aacttggcag ctagagaggt tttaccgatt
     2941 cctcccattc ccaacagcgc cactactcga cagcgttcct ggatcagcca ttgctccaag
     3001 agagcaagtt catctgtgcg tccataaaaa acagatacat ccaccatctc tccccaatca
     3061 aggcgcttgg ggttattcaa gccttcctgc ccttttaaat caaagtctgg gcttgtataa
     3121 tcgcttttat ccagttctaa attaaacatc ataaacaaac gaacaagcgt ccgtttgtca
     3181 accccggttt cgcgagttag tatcttccta aaggtgacag gagtgatccc agcctgttcg
     3241 cttaattctt ctaaggtaaa cttggagcca ttgttgttcc taaattccca ctcaagtttg
     3301 gcattctgaa gtttttgcca gccttcacgg gtcagtataa gcccacgatt gcgtttgggt
     3361 ttctcctgtt tcataatttc ttaggaagaa ttgtatttgg agtaatggct tctacagttt
     3421 gcgtatatga aaagggattc ttaataaatt agagttgttg ctaaatcagc taaaaaatcg
     3481 gtaaaaccca gtcacagtta aaatttaact ccacttgatt tagaatagtt aaaacgtaaa
     3541 gattacaaga ttttgagcta ctttaggcgt tatttaagga acgtggattt attaacttac
     3601 aattcttgat cgtcggctaa cctggtattt aaaagtcttt aaaccaagtt ttgcagttta
     3661 tttaatccac atttctaagg gacaattcta ctattgccaa acttagagtt tattgataaa
     3721 atacatctta agttttagac tacattaatg cagtataaag caacatgaat agcggtgtgt
     3781 taaggggcaa atattcgtga attaatccag taattacacc tcccattgcg cctaaagcac
     3841 cctacttaaa ttgaatgttt gttttctaaa ttacctctaa agaaggaata aatagagcaa
     3901 tctgttaccc aaaaccccta attatatgga taaatatcca ccgcataggt tagcaggtat
     3961 tgactctaat gggagcaaat ttgaatgcac tatcctaatt ttaggatcat ctttgcatcc
     4021 agtaggattc gtatgtaata gtaattgaaa agtaattaaa agtcaatgac aatggccaga
     4081 aaaaatgctg ctaatttaac gattcagttg tcgctggagg agaaaatcat tttggaggaa
     4141 tattgtgata aaatgcagcg aacaaagagc gatgtaattc gagagatggt acgctcactt
     4201 aaaaaatatt tagaaggcag ctaaaattgt cgataagttt ggaaactcat atttagaaag
     4261 taagctttca catatttaat ttttccgagc gaaattcgcc agatagggaa ttcagcctag
     4321 ggcggccgca taatgtcaat aagcaaagca tactctcaac agagttaaag ctaatgtcat
     4381 taaatattgc ttattgcgaa aattttatga cttggcgtag cccgccgcag gcagcacttt
     4441 gaccaaactg gtgcgcgtgt tggcagagtc aaccacacaa gaaatgtaat ttgtaaccct
     4501 ttatatacaa tctatcaaac gacacgaaaa aaagaccgct tgagcggatt aaaagtattg
     4561 cagaatacta aagagttgaa gtttatcctt tcggagttga catacgaatt attagagact
     4621 ttcaatctcc caactaagtg gattaatcaa ctcaaattat taccgactct taccgccttt
     4681 accgaaactg agtttaatga gttagttgat cagtatttaa ttaaattagg ctcggtaagt
     4741 cgtaaccgga tatacgaagc agcagctatt actttttacc atcagcaatc ggctattcct
     4801 gtcatcccaa ttctactgtg tgacgatgct ccacagttca agctcataac cgataattta
     4861 gctctttgtt gggtgcatga aggtagacat tataaaaagt taagtccatt tattgcctct
     4921 caccaaaaga ttctagatga ttttctctca cgattctgga aatattaccg aaaattatat
     4981 tgaggggata caaatatgga ttaccaaaaa gaagagtttg agagtaaaaa attagatcac
     5041 ttaggaatag taacaggaat aattgatgat atagtaattg tcgaaaaaat caagaatata
     5101 ttcttaattg atagtagaga aaacatcaat acaggagaag tagtcaaagc aattattagc
     5161 tatatgtagc gacctgcaat tataaatcac ccaacctgca atcatcgttg ttctcagcca
     5221 ggattatttg caactataat tgcgcttcag ccaggattaa ggttcttggc aacttttggt
     5281 gatcttggtt ggggtaaggc agacggcaga cggcagtccc aatgaaaaaa tttcatcacc
     5341 atgaatgaaa atgtggtaac ttccaagata aggctgatac aagcgaggtt gtttccttat
     5401 tagagcacca gagcctgaag ggcagtctaa cctggatatc gagaggaatt accaattgtc
     5461 gccactgagt aattttttgg ttttaatgag cttcgtccgg atggcacaag gaaatacgta
     5521 cattcactac aatgactcag gacttgttaa aactttctga ctggttaaca agtcacgatt
     5581 gcactcatgt agcaatggag agtactggcg agtactggcg acctgtattt aatatcctag
     5641 agggaaactt tgaagttatg ttagttaatg cccgtcatat aaaagcggtg ccaggacgta
     5701 aaacagatat caaagactcg cagtggattg ccgaactact acaacatggg ttgttacgtg
     5761 cgagttttat tccccctgtg gagcaaagag atttgcggga tttaactcgt catcgtagca
     5821 attttattcg agaacgagtc aatttggtca atcgagtcca aaaagtgctt gaagctgcca
     5881 acattaaact tgcttcagtt gccagtgatg taatgggtgt atcaggacgg gcaatgctag
     5941 ctgcgattgt tgaaggtagc gctagtcctg aactaatggc ggacttggca aaaggaacta
     6001 tgcgaaaaaa gcatgattta ctgattcaag cactcgaagg tagagttcgt cctcatcaac
     6061 gatttattct cgcacaccta ctgtgtcaaa ttgatagcat agacgaaaca attaaatgtt
     6121 ttgatcaaca aattgaggaa tactgctgtc cttttgagca agcggtagag ttaattgata
     6181 ctataccagg tatcgcacgt cgaactgcgg agattattgt ctccgaaatc ggcatcgata
     6241 tgagccgttt cccaagtgcc cagcattttg ctgcttgggc tggggttgct cctggtaact
     6301 acgaaagtgc tgggaaaaag ctttcaacta gtacccgtaa aggcaatcgc tccttacgaa
     6361 caattctggt tcaagccgct cacgctctag ctcatactaa aacttacctt gctgacttga
     6421 aaatactcct tcgatgagta tctatataga tccgtctcac atcacatttt cattcatggt
     6481 gatgaaattt tttcattggg actgccctct gcctcctgcc ttctgcctta aagcgcgtaa
     6541 cctttcatca ccaaaatctg acaagaacct taatcccggt ttgtaaatgt cttgattgca
     6601 ggtttgagcg attagtattg caggtcgcta cagctatagt aaagaaaaat taagtcctgt
     6661 ttggtattac actggttcag cagcgggaac tactaaaagt cttggtatga ggacgtttcc
     6721 tggctgctct aaaatgaaac gaacacaacg agcgatgtcc gcagattgta gtgcacctcc
     6781 cgaatagacg aaattttttt gctcctcacc ccatttgtcg tataagccag tgtccacagt
     6841 acctggggca atgcttgata cacgaatatc ggttcctgcg agttccacgc gcagcgaatc
     6901 cgtaaaggct tcaatggcaa atttagtacc gcaataagca gctagttgag gaactgtttt
     6961 tagtccagca atacttgaag tattaagtaa aaatccacta ccggattcct tgaagtgccg
     7021 taacgctgtg tagcctatcc tataagcagc ttctacattg actcgaacca tctgacagat
     7081 tgcttcgata tcaacacctt caattggtcc cacagtcatc acgcctgcat tattgaaaac
     7141 tacgtcaagc tgaccaaagg tttggatggc aaagtctact aatttttgcg gcaattcagg
     7201 gtttgtgatc tcaccaggaa tgactttggc atgttgtaga tttgacgcaa ggcgttcaag
     7261 tttctcttgc gatcgcgctg tgagtactaa attcatgcct gctgcgtcta gctctttggc
     7321 aactgcttcg ccaataccac cactagcccc agtgataata gcaactttat tttgtaaatt
     7381 catgcatttc tcctttaagt ctgtcgatgg atatcttact tttgacttcg gtgattttaa
     7441 tgttttctca ctaataactg gaattaatag cagaaaaact gtagtcactt aagcatgtat
     7501 ttatcgtcta aggaaaaatc aggacaacca tagttattta cctatttttg ttgattgcaa
     7561 acctgtattt cctttagtct ataaattaca aaacacaatt gcaatggtta aagttttgaa
     7621 gaaagtttgt attctttcgg ttctagagcg tgttatcagt tgagccaaat cacagtagca
     7681 gcgaggtaaa ttgcaccgag aaagttgaat ttgagcttgt catagcgggt ggcgatcgca
     7741 cgcaatcagg ttggcagttc accgagagta aaacgcactc tctctcaacc ttagcagtga
     7801 gcgatgccta cggtggcaag ctacgcagca ttgcatcaac gataatcacc tgttccatac
     7861 caataccttc actgttcaat agctttaaaa tctgtagcgc ttatcccgca tagcttagag
     7921 cttttcttag tgccattcgg ctattaagta agttggtaca ataaaaccaa actatgcaaa
     7981 gaaagataaa ttaggctaca atctttttgc ctgttacctc ttgcgttttg atttattcca
     8041 cctagaaatg ttgacaccaa cctacttaat atcttgtgca atgattttgt ctaaacaacg
     8101 ttctgctaaa gctgcaatgg taaaagaagg attagtacaa gcagcagaac ctggaataag
     8161 tgagccatcc acaacataaa gtctgttgta tccatatact cgcccaaatg ggtcgcaagc
     8221 ttttcctatg ggaactcctc ctaaaggatg accagtaata gcagcatcat tattttgaga
     8281 tgttgctatt ggagatgtag agatcatacc ttctttaatt ttatgccgag gtcgtctacc
     8341 aaaattattg atgctattgt caagggtact gaaaagtttc tctgtctttt tgacgatgcg
     8401 atcgtctaca gatgttggcc aatttaagtt gacggtatct gtagatggat cgtagttaaa
     8461 cttaccagct ggacgaggaa tacccagacc cagacaagat tgtattccgt cagcattgtt
     8521 ccccagttcc aaactctcta caactatcgg agtcggcccg tcaagctgtt caataacagc
     8581 acccgcagtt cctccattac ctgtgacagc acctaagtcc gatctaatgg taatgttgtc
     8641 tccattagtt ccccaatctt tcccaacaga gttgtttaag taaggtaatg tccctttttc
     8701 tctggccttc actaaaagtt tagaagtacc aacagagcca gctgctacga ataaataacg
     8761 gcaagtaaag gtttttttcc gaataatttg ccctgattca ttgatttcat tgcagaaaac
     8821 acgatatttt ttctcaccac ggatctcggc aatatctgta actacgtgta gaggtaaaat
     8881 atctacgtga ccagtatttt ctgcctgggg aagataattt tgatctaaac tattttttgc
     8941 tccgctatta attccccacc aacattgacc atctataaca gaaggtactt ttgagccatt
     9001 aatttcttga cgaactgtat cccaatttac agctagattc aggagaaaac ttggcaagcc
     9061 tgccttgtct gcattctcta ggaaaaatcg agttttgcca tagtaagtag tagctaaaac
     9121 atcctgtgga attggtgatg atttcagcat ttgttgaact cgaggataat aaaccgaatc
     9181 tagttcatca tagtcaacag tagcacggtc aaatactttg taaaatattt cgcgcttagg
     9241 tttataagta atagcgttat aaactagtga accacctcca acaccagcac cactgacaac
     9301 aataattcct ttttcttgta gtgtttcaaa gactcctgta tatatatcta cagtatcacc
     9361 aaacacagat gttgaattta gccaagaggc tctaccatca ggattattga aagtagcaaa
     9421 agtattttgg ctagctgtaa taggccaacg cttgcctcgt tctaaaatca atgtgttaac
     9481 accagcttgt cccaaacgta aagcagcaat agccccacca aaaccgctac caattactag
     9541 tgcatctata taatcatcac tagcacgagt ccgaatggga gaggcaactg aggctgctaa
     9601 actcgcagaa aatagggcag cactctgaat aaagtggcga cgatgaaatt ttttatcaaa
     9661 catacaagtt attcaaatgt tgatgataga ttcttactag aaatagctga agcaattcaa
     9721 aacagtttag gagtttattc tcttcttaaa actgctagat gatagacact aatatagagg
     9781 agattacagt tatcttttct cagtaaacaa acaaattaga ttactcaaaa agtatcgcct
     9841 aaatattttt taagagcagt actagtgtaa ttaattctat ttttactcag atttatcatt
     9901 aataacaatc gccttaatat cagtgaatta ttgtagtttt tagacttaga taaagagtaa
     9961 tttcgggcgt gtttgctaac acgccgtatt tattctctag ttacccaact actcctgtta
    10021 ctgtattcat cgcaggttga cggaagcgca aggaagtaca ccaccaccac caaccatgtg
    10081 atgctttcca ccactcacca acagtcgcac tgctatcaaa tttctcaaat tggggattct
    10141 taggaagaat aaagtagata agatttgagg tttcttctaa tgactgaatc ttcagttcag
    10201 cagggaaggg tattccgatt tctccttcaa atattgatct gggatcgctc aggagttgct
    10261 gacggaaagt ttcattagtc caagaacgaa ctatccataa cgcttgtaaa cactctctgt
    10321 aagctctagc tttggtagta ccaccaagtc ggttataaaa atagtagaaa gtatcgataa
    10381 ttggccacca gctagaaaac tgttcatatc gataccaatg ttcttccact ggtggagtcg
    10441 caggaagtac aaagtagaaa gtgttttctg gttcttctaa tactttgact tctgtatccg
    10501 caggaaatgt aattccggtt tctctttcaa aaacagttct tgggttagtt aatatctctt
    10561 gcttgagtgc ttcatcactc caaatacgat caatcagtcg tgtttgtaaa gcttcataat
    10621 aagggccaaa attggtaata tctacacctg accaaagctt tctaacttca gggaagttag
    10681 tagaagtatc tgaaatagga gcgtttgttg taaccatgat tagctatcct tgaaattaat
    10741 ggtatgggaa agatcaaact tgtttggtat tacacggttt aactgaccgc aactaccaat
    10801 tttctggatt tagggtgctg taaatttgac gctaaatctt ctgaatttgt cttatttcgc
    10861 cgtcgttaga tgagcttcta ttagtttgac gattatcatt attttaaaac ctaaaaaaaa
    10921 tgactacaag ggttaaaatt tcgtagctag gttacattgt tttggtgcag tggtttcaag
    10981 cgattattta ctcaggagta atcaaaaagg gtcaagaaag tctataaaac ttgctcaaca
    11041 ggctttgtcg cccttaaata tggttgataa tacttttgtc aatctaacta atttagtatt
    11101 taaattgatg attaaatgag attttactga ttaacataat ttctaaaatt agatccaaaa
    11161 aaatacaacc ttgctacgaa aaattgacct ttgcatctgt gttggatgcc tcataatctt
    11221 agattgtgac tcagaaagta ttcagcttca acttctatgt gaaagctata ctaatagcct
    11281 ctatgaagcc atattagctc gaatctatcc tctaaaatag aggtttatta tgccttttcc
    11341 cagatctagc ggcattttgc tacacccaac ttcttttcct agtcaatttg gtattggtga
    11401 cttcggatcg gaagcttatc gctttgttga ctttttagta gaaagtaaac agcagctttg
    11461 gcagatattg ccattgggcc ctacgggcga tactaattct ccctatgctt cttattcagc
    11521 gatcgccggt aatcccctgt taattagccc agaactgctg tataacaagg gtttactaac
    11581 tgaagatttc actaatttac caaagttttc cactgaaaga atagattttt cccaggttat
    11641 tcaagcaaag ataccaatac tgcaaaaagc ctgtaaaaac ttcaaaatca gtgcaaaatc
    11701 agaacaagaa cgggaattta aagagttttg tcagagtcag gcttactggc tagatgatta
    11761 tgccttgttt atggcactta aggatgccca taacggcgct agttggaata cttgggagat
    11821 ggcgatcgct caacgcaaac cagaagcgat cgaacaatgg gagcaacgac ttgcagatga
    11881 agttttttac cataaatatc tccagtttga gtttttctcc cagtggtcac aattgaagcg
    11941 ctatgctaac gagcgcggga ttcaaatact aggagatatc gccatttatg tggctcacga
    12001 gagcgctgat gtctgggcta atcctggaaa cttttgcctt gatgaacaaa caggtgaacc
    12061 agcattaatg gcaggaacac caccagatta cttcagtact actggacagc tttggggtaa
    12121 cccagtctat aactgggtgc ggctacaaca ggagaatttc aagtggtggg tgcaacgctt
    12181 ccagtccatg cttaataatg tagacttgat tcgcatcgat cactttcgag gatttcaagc
    12241 ttattgggca gtcaagcagg gcgaaacaac tgctatgaat ggtacgtggc ttgaagctcc
    12301 tggagaggct ttctttgaag tactcaaaca gaagttaggt aacttaccaa ttatcgctga
    12361 ggatttggga gaaattacac cagaggtata cgctctgcga gacagatttc aatttcctgg
    12421 catgaaaatt cttcagtttg cttttggcga tgcttcacaa gctgaaaagc gttttttacc
    12481 gttcaattat cagtctaatt gtgttgtata tacgggtact catgataatg acacgacagt
    12541 tggctggttt aatcaattgt tacccccagc aagagaacaa ctcttaagtt atttgggttg
    12601 tcagagtgag tctggaatcc actgggattt gattcgattg gcactgagtt ctgtagccaa
    12661 tcaggcgatt attcctctac aggacgtttt gggattagac accggagcta ggatgaattt
    12721 tcctggtaaa actgaaggta gttggggttg gcgatatcag tctacagctt taacgccaga
    12781 gattagcgat cgccttggaa ctatgactga aatttatgga agagtgagtg aataattcgt
    12841 aactcgtaat tacagcagtt ttcgacttaa acccttttat ccagaagaat aaaagggttt
    12901 atctactact tgcttaatcc ggaaattgac agaagctgtt tacggacgcg ctcgcttccg
    12961 ttatggatgg aagaaacaag tcaaatgctc ttcccttctg cctcctgcct tgtttcttag
    13021 gagcaaccgc actggcggcg ctgccacccg ctatgaatga aagaaactat tttgaggcga
    13081 ctaatcaacg ggtacattga taattatcct gacagttaaa accgaaccca caatagtgtg
    13141 ttgagcctga ttaggagcat ggttctgggg ctttatggct atgacattgg tgaaggaaaa
    13201 gtttcctatc ccgtttgaaa gattatctat aaatccatcc ccaggtcagg acttatccat
    13261 aataagcata tcagagttgc agctatgaaa ttgccaccaa atcaaggtca taaccagcct
    13321 gtacttacgg aaaaagtatc atcaacgtta gaagaaatta accaaaatgc agcaggaata
    13381 gaccttggtg gtggagaaca ttgggtttgt gtacccgcag atagaactga caaaaatgtt
    13441 cgcagattcg gatgttttac ccctgattta attgcaatgg ccgattggtt aatagaatgc
    13501 cgtgtagata cagtagcaat ggaagccaca ggagtttatt ggattccagt gtttcaaatc
    13561 ttagaagcca agggttttga ggtcaaatta gtcaatgctc atcacgtaaa aacagtacct
    13621 ggtcgcaaaa cagatgtgtt agactgccag tggttacaaa agttacatac ttatggacta
    13681 ctgtctggtt ctttccgccc acaagaccaa gtttgtgtgc tacgcagtta tatccgtcag
    13741 cgagacagct taatcaagag tgctagtacc catgtacaac ggatgcaaaa agcacttatc
    13801 cagatgaatt tacatcttca caaagtcatc agtgatgtca cgggtttgac cggaatgaag
    13861 attattaaag ccatcgtcgc tggcgaacaa gacccacaag ttttagccac actcaaagat
    13921 ccacgcatca aaagcagcac agcagatatt gctaaggcat taactggtga ttatcgtcgt
    13981 gaacatttat ttgtcctcca gcaagaatta accttatatg aggtttacca acaacagatt
    14041 agtgctattg acgctgaaat tgaaaaatgt ttagcatctt ttcaacctca gactttagat
    14101 gaaccgccaa caacgccaaa aaaacgtcgg aaaaaaccga ctgcaaatca cccaaatttt
    14161 gacttgcata agtatcttta tcggatggca ggagtagact ttacactgat taatggtctt
    14221 gatgctttaa ctgttcaaac cattttatct gaagtgggat tagacccaaa acgctttccc
    14281 acagttaaac acttcacatc ttggttgggt ttatgtcctg gtcaaaaagt gactggtggt
    14341 aaggtcaaaa gctctaaaac tcgtactgtt gtcaatcgtg ctgccaatgc tttccgcatg
    14401 gctgcttttt ctctgactca cagtcgttct gcgttgggtg ggttttatcg ccgtttacgt
    14461 tctcgcttgg gcgcacccaa agccattact gctactgctc acaaactggc tcgtttattt
    14521 taccggatgt ggatcactgc tgggcaatat tctgatcctg gtatggacta ctatgagcaa
    14581 aaataccagg aactgattct caaacacctt cagcaaaaag ctcaggcttt tggtttggaa
    14641 cttatcccta tttctgactc aacgcaatgt gtttcttgaa agaggggtaa gagggatgtc
    14701 aggaaaacac aaattaaaag cttggggttc aatcgaatgg acatgagaaa accaatctga
    14761 accacttaat cttatgccca ttgcaccagt ttcacttaga gcttttcgta ttagccccag
    14821 ttgatgataa atttcttggt tgatcgaatt catagtaagt caaaatcaat ctataaaaaa
    14881 ttgctagtca ttatttataa tgctccaaaa caactgatca taaaaatgta taaaataata
    14941 tgtattactg aattttttta aattatgaaa ttaattataa aataagtttc agtattttta
    15001 gagatattat taccaaaaaa agcaacctta aagcgaaaaa ataaccgttg tgtttttgtt
    15061 taaaattaat tgatttagta tttaatcaat taaataaaaa atgtcaacct cctacaaaac
    15121 attaaccctt gtagttatat atttatctct ctaaactttt agataaagac ttaaaagtaa
    15181 gtcaaataca ttccaaatta agaatctagt gaccctaaga atattgaacc aatatgatac
    15241 ctagatccct tggtgggaaa aaaatcgcaa ttcttctaga aagtgagttc attcctgaag
    15301 aaattgaggc atatcaaaag cgcttttcag aacttcaagc aacagtacat ctgatgtcta
    15361 ggctctggaa tcagccgagt gttcgctttt tcagtgatga agatacgggc aatacaccta
    15421 aaaccataga cgtaaacatt gattttcaaa atgtagatgt taacgattat gcggctgtga
    15481 ttatgtccgc taactatacc agtgttcgtc tacgttactt tgagccacca acaggtcagc
    15541 caattagtgc agaacaagtt cgcacttcac cggctgtaca attttatgcc agcgcaatgg
    15601 taaatcccag gattattaaa ggcgcacttt gccacggtct ttggattgtg acaccaatac
    15661 cagaattgct caaagggcga caagttatct gccacgaggt agtccttgca gatatcatta
    15721 acgctggtgc aatttatact ccatcacctt caggtgttgt ggtggacggt gaccttgtga
    15781 ctggtcgttc aaaacacgag gttgaaccat tcattgatgc gattactgaa caaatccaac
    15841 aattatctat tgccactaat cgtttctcag gcagaagagc tactacctca gtttcaaagc
    15901 tgaaagttgc cagctaaacc ccatcatttt gaaaccgaga gtttttaagg agagtatatt
    15961 attcggtcgt ggcttgagtt ctgagcttta ccttaattcc aaaaactatg gttatttaag
    16021 gtaaacgcgg tttaattatc tcaaaacaaa ttcatgatta ggagacaatc ttgtgaaaaa
    16081 aattctcata attttatcgg aatatggtta ttggggtgaa gaactggtag gtcccctaga
    16141 aacttttgat gctgcggggt atcaggttga ttttgcaact ccaacaggaa aaagaccagt
    16201 agcgcttcct ccgagcatgg accctactta tgttgatcca cctttaggtc ggccagttgt
    16261 ctctcaagag atggctgaaa aagtcaagcg cttagaagat cctaaaaatc cacggttaga
    16321 caacccaatc atcctgtcac aatggttgcc cgatcgccct tactggagtg ccgaaaaatt
    16381 cctgcgggaa atggaaacat ataacaatgc tctcgacgaa atacagccag atttggatca
    16441 atacgatgca ctattgatcg tgggtggtag tggcccgatc gttgatttag tcaataacca
    16501 tcgcgttcat gacttgattc tcagctttta caaaaagggc aaagcgatcg ccgcagaatg
    16561 ctacggtgta acttgtctag cctttgctcg tgacttagca gaccgcaaga gcattattca
    16621 gggtaagcac gtcacaggtc attgcaagga atatgattac aaagatggta cagggtttgt
    16681 cggcgcagac atcaacatgg gtccacctcc ctatcctcta gagtatattc tccatgatgc
    16741 tactgcccca gacggagcat atcacggtaa ttttggcaaa gagatctctg tgattgttga
    16801 ttatcccttt atcacaggtc gatctaccgc agactcttat acaaccggtc agaagttagt
    16861 ggaagttctc gataaaggac tgaggcggta cggatggtaa gcttgaccaa acagaccaac
    16921 gaaaagcctt tgatggctaa cagaaatggt cgctttgcaa ttattgaaca acttctggca
    16981 gatggtatag agtacatgtt tggtaaccct ggcacagttg agcagggttt cctagatgcg
    17041 ttaaaggact attatccaga atttaagtat atctttgcac tgcaagaaac aatcgcagtc
    17101 ggtgctggcg atggctatgc ccgcgccacc aaaaagccta cgattgtcca actccatagt
    17161 ggagtcggtt taggaaatgg catcggaatg atttatcagg ctatgcgcgg tcatgcacca
    17221 ttagtagtcc tagctggcga atctggtatc tgctacgatg cgatggatgc ccaaatggca
    17281 gcagatttag tcagcatggc aaagcctgta acaaaatggt caactagggt cgtcgatccc
    17341 tcttctttgt tacgcgtgtt acgccgagcc atcaaaatag ctgctacgcc gccgatgggg
    17401 ccggtgttcg tctccctacc gatggatatc cttgatgctg ttaattatga agaggttgtt
    17461 gcgacttctt ttccagtgac aagggttgct cctgaaccag agcaactgga aacagcagca
    17521 aatttattgg ccgcggctaa acatcccctg attattatgg gcgatggtgt agctttttcc
    17581 gatgcccagg ctgaattgac atctgtggcc gaactcatcg gcggacaagt ctggggagca
    17641 gactcatcgg aagcgaatat gagtgctagc catcctctgt tcggagggtt actaggtcac
    17701 atgtttggtg atgatagtcg tcgggttaca tcccaagcag acgtagtctt gattgttgga
    17761 acctacgttt ttccagaggt attcccagcg ctgtctggag tttttgctcc ttcagccaaa
    17821 gtaattcaca ttgacttgaa tacttacgaa atagcgaaga attttccggt ggatcttggg
    17881 ctgcttggcg atccgaaaac tagtctgagc aaattagcca tcgcccttga atcaactctg
    17941 acacccgaac agaaaaaaga ggtttttgtc agagctagcc aaatagctga gactaaaaag
    18001 caggaatcgg tggctcgact tgaggctgac aaggcagttc gggatgcagt acctctgcac
    18061 atgactcgtt ttgccgagga attagctgct catttaccag cagatgcgat tattttcgat
    18121 gaagccatta ccaattctcc ggaactttgt cggtatctac caccgacaaa actcggacat
    18181 tacttccaaa cacgaggcgg ttcgctgggt gttgggattc cgggagcgat cggtattaag
    18241 ctggctaatc cagataaaac tgtgtttgcc ttcactggtg atggcggcag tatgtatacc
    18301 atccaagccc tctggactgc tgcccaccac aatatagatg ccaaatttgt catctgtaat
    18361 aatcacagct atcaaattct caagttgaat attctgcact actggggcga gcaacaaata
    18421 ccagaacacg actacccatc ttcttttaac ctctgcaacc caagtatcga tttcgctgaa
    18481 ttagcaaagt ctttaggggt tcaggggatt cgggtagaaa ccccagatca gattgcccca
    18541 gctatccaaa aagcattgga tcataacggg ccatttctga ttgatttggt actgacaaat
    18601 gaaattgtcg gtacaaagat cggtgtcaag tgtggtcaat agtagaccgg aatttttact
    18661 gaattaacag ggagaaaagc aatgacagta gctaaattag cacctttaac agaattagaa
    18721 atcaagcaat tagcagcaga ctggtatttg aaactagatg ttcacgctcc gctagaagat
    18781 tatattcctt ttctagctga agatggtcta gaaatgcgct ttcctgaagc tacagtttat
    18841 ggttttgatg gatttaaagg ttggtacgat cgtgttatcg ggattttctt tgatgaagtc
    18901 catactttaa aggaagtcaa cgttacgcct tcaggagacg aggcgagtgt tcaagtcatc
    18961 gtcaaatggg aagctagcgt gtggaatcca ccagcagcca aaagtcaacg cattgtttta
    19021 gatgcttatc aaacttggat cgtgaagcga tcgcctaata ctggtaagcc agtaattgca
    19081 acctacattg tcgattcact tagttattat gaaggatctg cacgtcttta agattgcaga
    19141 catccgccaa aaaacaaagt tttggggtgc atataacaga ttgtatgtag ttaatagcgc
    19201 tccaaactta taattacatg aaaattacag aagataaaaa aatgacggtt tcaacaattt
    19261 cacttgggcg gaaattcttt gacaaacata tcgagactat ttctgccgga aaaatcgatg
    19321 aaatggtgga tagggattat gcagaagatg ctgtattaat aacctttttt aatgggtttg
    19381 aaaatacacc ggcacccatc acagttaatg gtcgtgaaaa tatcaaaaca ttctttcacg
    19441 attacctgag aacaatcgaa tatattgata tcaaatctct ggacttcact gaagcggaag
    19501 acgctatatt ttttcaagcg aaattcaact gtaaacttgg tcaagtctca gttggagatg
    19561 cttggactct tcgtaatgga caaattgctt atcactttgg tttttgggtt tcctagaata
    19621 gcactaattt taggagatgt ttacggcagt aaactacgcg cttgtttgta gtttgcaaat
    19681 agtagtctac cacagtaact ttacctggta tgtagtaaac actaaagtgt ttactacata
    19741 cttaacttaa gtgcaactcg gttttatttc catcctcacc ataatttgtt tcagtcggaa
    19801 taccctacaa tagaggatta ctatgagtac acccatagaa caattgcttg tatctcccat
    19861 cagcaagctc tacaaagagc atatcggttt cattaaagcg aagaatgttg atggtttact
    19921 caatcagtat gctgatgatg ctctgctgat tagcacctta actgaagacc gccagccgct
    19981 ctacgtgcaa ggtcgtcaag cactcaaaga gttctttgaa ggtcgcattt tcggtttaga
    20041 agattttgaa atcaggctca accagtgggc tgaaactgaa aacacgctga tgatggtaga
    20101 agagttaaaa accgcaagtc aaactggtga agtcggtagt gtagagtttt acgataactg
    20161 gtttttgcaa gatggaaaaa tcgctatcca ctttgccggt gtaattcagt atcctgataa
    20221 aacttatgta aatgcaggaa cagttaaatc agaaccacct gactctccat tgggtaagtt
    20281 ttataaagag catatcggct tcattaaagc gaagaatgtt gatggtttac tcaaccaata
    20341 tgctgatgat gtgctgctaa ttagcacctt aactgaaaac cgtaaacctc tttatgtacg
    20401 tggtcgtcaa gcgctcaaac aattctttga aagtcggatt ttcagtctag aagatttgga
    20461 agtcaagctt gaccagtggg ctgaaacaga aaatactctg atgatagttg aaagcctgaa
    20521 aaccagaagc gtcaatggcg atgttggcga agtcagtttc tatgataact gggtgttgcg
    20581 cgatcgcaaa attgctgttc actttgctgg tgtagttcag taccccgatg gaacatacgc
    20641 ataactacag cggaattcag gagcggagcc agagacgctg cgcggcttgg cgtcagaatg
    20701 ggctacgccc cgctgcg
//
