LOCUS       NZ_CP054919            11276 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Kitasatospora sp. NA04385 chromosome, complete genome.
ACCESSION   NZ_CP054919
VERSION     NZ_CP054919.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora sp. NA04385
  ORGANISM  Kitasatospora sp. NA04385
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 8400742)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Genome mining for natural products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8400742)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Institute of Functional Biomolecules, State
            Key Laboratory of Pharmaceutical Biotechnology, Nanjing University,
            No.163 Xianlin Avenue, Nanjing, Jiangsu 210046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013364235.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 08:11:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,532
            CDSs (total)                      :: 7,423
            Genes (coding)                    :: 7,206
            CDSs (with protein)               :: 7,206
            Genes (RNA)                       :: 109
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 81 of 218
            Pseudo Genes (incomplete)         :: 153 of 218
            Pseudo Genes (internal stop)      :: 9 of 218
            Pseudo Genes (multiple problems)  :: 25 of 218
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:10:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5782780
            Orig. end                         :: 5794056
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6276](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6276)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            690..2102
                     /gene="hemG"
                     /locus_tag="HUT16_RS25690"
                     /old_locus_tag="HUT16_25690"
     CDS             690..2102
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138585.1"
                     /locus_tag="HUT16_RS25690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25690"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_176190415.1"
                     /sec_met_domain="DAO (E-value: 4e-08, bitscore: 32.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKPHVVVIGGGIAGLAAAAFLGDAAVRVTVLEASGRFGGKLRGG
                     EVGGVRVDLGAESMLARRPEAVELARRVGLGERLEPPTTAKAAIWTREGLRPLPGGQLM
                     GVPGDLDALRASGVLTPEGLERALREQPAEIGEDAPIGRYIADRLGREVVDRLVEPLLG
                     GVYAGHADEISIRAAVPQLLQLAGQGGSLTTAIGELGRRATATGPVFQGLDGGLGTLPG
                     AVAAHDRAHGAELLTDHPVDALRRTPDGWRVTAAGRELAADAVVLAVPAPQAAALLAAD
                     APGAAAELRQVEYASMALVTLAFRRADLAGSPLTGSGFLVPPVDRRSIKASTFSSNKWG
                     WLERSAPDAFLLRTSLGRHREDAALDLDDAELVARSLADLRAAVGLRATPYDHAVTRWR
                     AGLPQYPVGHLDRVARIRTRLDALGAIAVCGAAYDGVGIPAVVAGARRAAEHLAPATPG
                     TGTTRTEGTMGP"
     gene            2099..2809
                     /gene="hemQ"
                     /locus_tag="HUT16_RS25695"
                     /old_locus_tag="HUT16_25695"
     CDS             2099..2809
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138586.1"
                     /locus_tag="HUT16_RS25695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25695"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_176190416.1"
                     /transl_table=11
                     /translation="MTESTEQPVKKKARDLNQVIRYTMWSVFKLKDALPEDRTALAAEV
                     DALFEQLTAKDVVIRGTYDVSGLRADADVMVWWHAESSDDLQEAYNRFRRTGLGRHLEP
                     VWSNMALHRPAEFNKSHIPAFLADENPREYVCVYPFVRSYEWYLLPDEERRAMLAEHGM
                     MARGYPDVRANTVASFALGDYEWLLAFEADELHRIVDLMRDLRPSRARLHVREEVPFFT
                     GRRKPIADLLAGLY"
     gene            complement(3054..3815)
                     /locus_tag="HUT16_RS25700"
                     /old_locus_tag="HUT16_25700"
     CDS             complement(3054..3815)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847913.1"
                     /locus_tag="HUT16_RS25700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25700"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_176190417.1"
                     /transl_table=11
                     /translation="MWVLLLIPSCAAAVLSCLRLVRVASIADALAGGTPHPVHRPAGVG
                     LYETAYLAGGPARVVDLALVRMAGVGRLHLAHTGWTTVVDPRGRGRLERALIGAIGPEG
                     QCRTAQLKTVLAAHPTVRDIGDRLTSAGLATPAVVRDHALRAVRQVRYALVGAAAMLLL
                     TVLLTAPDGTAARTAAAWYALPLVLTGGTLLMARIDVHAYTAWAAPAGQTLLRALPCRH
                     APATDDELLTAVAISGPAAVRDPRLRAALDS"
     misc_feature    complement(3820..3835)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.4"
                     /tool="antismash"
     gene            complement(3897..4964)
                     /locus_tag="HUT16_RS25705"
                     /old_locus_tag="HUT16_25705"
     CDS             complement(3897..4964)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="HUT16_RS25705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25705"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_176190418.1"
                     /transl_table=11
                     /translation="MWNFRFVLAFALVAFTLALAAVRNRRSRRVTDPQGLPGRGQPLLD
                     VAFLAGGPARAVDTALVRMEREGRVAISRAGRVTVTDDRTSDPVERTLITAVGGRAGRD
                     LSVLRASVTGSPEVQRIGDGLAARGLMRHPGALRSARRARLLVVLALLVAIVLGTVSTV
                     QWAGSAGWDTTAPPFIPFIPLVLLTVVVLVASRVPRRRITPAGLRQLQLVRGKGPWRPH
                     EVDRAHAAVVGAFALDGAGSLRKPELRALRAALEGPAAATRSGRSSRGSGADGTPEFAA
                     GAGAGSAAWCGSTPASSCGSSGHHSGGHSGGHSCGSSSHSCGSSSSHSCGSSSHSCGSS
                     SSCGSSCGGGGCGSS"
     gene            complement(5001..6276)
                     /locus_tag="HUT16_RS25715"
                     /old_locus_tag="HUT16_25715"
                     /pseudo=""
     CDS             complement(5001..6276)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958301.1"
                     /locus_tag="HUT16_RS25715"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="HUT16_25715"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 9.2e-88, bitscore: 292.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSSDIPRLGTGIGWRPEIDTVVERLPGLDWVEVVAENVCPGHLP
                     PSLAELRARGVTVVPHGVSLGLGDATAPDPARLARLAAVAESLGAPLVSEHLAFVRAGG
                     LEAGHLLPVPRTREALRVVAANVRIAQRELPVPLALENIAAQLAWPGDELTEAQFLAEL
                     VERTGVRLLVDVANLHTNRVNLGVDPAAELDRLPLEALAYVHVAGGVERDGVWHDTHTH
                     PVTAPVLEVLAGLCARTDPPGVLLERDGAYPPPAELSAELDAIRRVQHLHRRETARV"
     misc_feature    complement(6471..6485)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.01"
                     /tool="antismash"
     gene            6476..7396
                     /locus_tag="HUT16_RS25720"
                     /old_locus_tag="HUT16_25720"
     CDS             6476..7396
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573014.1"
                     /locus_tag="HUT16_RS25720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25720"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_176190421.1"
                     /transl_table=11
                     /translation="MTSTPYRIRPFAPADAADAAAAYRAGRPHLVTTPEVVAWQAELPH
                     YHLLVAERADRVVGTARFGPVPESTTSHQGFLNISVLPEHRGTGAGTALLAAAERDLAA
                     LGVRDAHSWVDDTPEALGFAAARGYRRSGRAGRFSGIDLTAPLPPLPPTPPGVELRTAA
                     DFLDDPYPIYLVDIDGTRAEPGDLVLDDQPYEGWLAEIWQRPDQDHTLTAVVLADGEPV
                     AFSAAQTDGHRTYWSAFTATRSSHRGRGLAGLAKAHSLHRAKERGFTAAYTQNDAGNAP
                     MLAINARLGYRECAAEWKHTRAICE"
     gene            complement(7441..9648)
                     /locus_tag="HUT16_RS25725"
                     /old_locus_tag="HUT16_25725"
     CDS             complement(7441..9648)
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1296: tellurium
                     resistance protein, TerD"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF014404.4"
                     /locus_tag="HUT16_RS25725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25725"
                     /product="restriction endonuclease"
                     /protein_id="WP_176190422.1"
                     /transl_table=11
                     /translation="MWSVLAQAQREQHRRAEAQRRAADARQRENERARREAQRTAARGE
                     REALKAYQQGRESDAARRSAELDARVAELRGVLAAGLAGPGFSLAAGGRPAVPPFDPGP
                     LGVPVPMPDQAWYLVPPPAGHLAYDPGARRQWEEHSARARAAFEHDWQAAWAAEQQRQR
                     QLADYRAQYDAWAAERHRLLAGQSAQAGQLAARLRAGEAAAVAEYFEAVVDWREDWPDG
                     FPADGDTAWDAPARRLVVRWELPPYEVVPAVGRYRYVRSDDREDEVARPATQRRELYRE
                     MLAQCALRVLAEVFRADTGGLLASVGLNGVVVATDPATGREGERCLLAVEADRAVFEAL
                     ALDRVAPLDCFVEALGGRIAARPERADTVAEVPAAAPAPGVQDPDGAEGANGPDGADGP
                     DGPDGADPDLFEMDPLEFEELIAELFRRRGLRTSTTARSGDEGVDVLAEDPDPITGGKI
                     VIQAKRYRKTVPPSAVRDLESTMRHQGANRGILVTTAGFGPSSRRHVENKPLTLVDGPM
                     LLELLREQGLPGRLGPAAPAAPAVVPAQRSAPAAPTTPATPTLPAVPAAPAVPATELAP
                     GQNLVLPDGEVRVRFRAGGAAADLTLLLLDADGKVRRDEDFVFYHQPVAEGGAVALRPD
                     DRSATVLTGRLPDAVRRVAISVNLDADGDGTCADLVDPAVELASGTGRWVFRPPADPAV
                     SAMLVAEVYRHPADGWKLRAVGQGWADGLAGLARDHGVDVA"
     gene            9812..10618
                     /locus_tag="HUT16_RS25730"
                     /old_locus_tag="HUT16_25730"
     CDS             9812..10618
                     /NRPS_PKS="Domain: Abhydrolase_1 (22-247). E-value:
                     7.8e-33. Score: 106.0. Matches aSDomain:
                     nrpspksdomains_HUT16_RS25730_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105916.1"
                     /locus_tag="HUT16_RS25730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25730"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_176190423.1"
                     /sec_met_domain="PF00561 (E-value: 4.5e-25, bitscore: 87.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.5e-22, bitscore:
                     80.0, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHTTVNGATLHHDESGPADGTPVVLLHGHPFDRTLWEPQVRALA
                     AAGYRVITPDLRGYGRSEVTPGKVLLADFADDTAALLDRLGVERAVVGGVSMGGQIAME
                     FHLRHPGRLLALVLSDSSAPAETEEGKEVRNRMADRLLAEGMAGYADEVIDKMLAPYNV
                     TALPDTAARVLAMMRATDPRGAAAALRGRAERPDYRETLRTVTVPTLLLVGADDVYTPV
                     ADSEAIRALVPHADLTVVPDAGHLPGAEQPERFNAALLDFLRTRLS"
     aSDomain        9878..10552
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUT16_RS25730_Abhydrolase_1.1"
                     /evalue="7.80E-33"
                     /label="HUT16_RS25730_Abhydrolase_1.1"
                     /locus_tag="HUT16_RS25730"
                     /protein_end="247"
                     /protein_start="22"
                     /score="106.0"
                     /tool="antismash"
                     /translation="PVVLLHGHPFDRTLWEPQVRALAAAGYRVITPDLRGYGRSEVTPG
                     KVLLADFADDTAALLDRLGVERAVVGGVSMGGQIAMEFHLRHPGRLLALVLSDSSAPAE
                     TEEGKEVRNRMADRLLAEGMAGYADEVIDKMLAPYNVTALPDTAARVLAMMRATDPRGA
                     AAALRGRAERPDYRETLRTVTVPTLLLVGADDVYTPVADSEAIRALVPHADLTVVPDAG
                     HLP"
     gene            complement(10692..11096)
                     /gene="msrB"
                     /locus_tag="HUT16_RS25735"
                     /old_locus_tag="HUT16_25735"
     CDS             complement(10692..11096)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138594.1"
                     /locus_tag="HUT16_RS25735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_25735"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_176190424.1"
                     /transl_table=11
                     /translation="MSYEVQKSDAEWRAELTPEEYHVLREAGTERPFVGEYTDTKTVGV
                     YACRACGAELFSSETKFDSHCGWPSYYSPLAGDSVEYLEDSSLGMRRVEVRCKKCGSHL
                     GHVFEGEGYDTPTDQRYCINSISLTLKPAE"
ORIGIN
        1 gtaggacgcg gacgggacct tgagggtcag cgtggactgc agggtgtcgc cggtgccgcc
       61 gcgcaggttc tccccggcga cgtacccgcc cgccccggtg gccaggtcga ccgccttggt
      121 gcgggcctcg tcgaccgaac cgacctgcac ggtcagcttc gcctggtagg cgatctggcg
      181 ggcggccgcg ggcgcggcgc tcccggccgt gctcccggcc gttcccgtgc ctgtgccggt
      241 tcccgtcccc gcgccggtgc ccgtccccgt ggtcgggccc gccgccggcc cgccgccggg
      301 cccgccttcg ccgcgtcccc gctcccggcc ccgggcggcc ccgccgcgtc ggccgcctcc
      361 ccgtccgccg cccgggcgtc gttccgggcc accgccgggg cgcccgccga ggccgacgag
      421 ccgtcggagc ccgcgctgca ccccgccagc agcaggaccg ccgccaccgc ccccgccgcg
      481 cccgctctgc gtacccgaag gtccatgtcg catcctcccc gtgtcccggg acgggccgac
      541 cgcccgcccc cacgcggttg tgacgcggcg gcccccggaa cggacccgca cccccggtac
      601 cggatcgatc acgccgcgga ctcgcccggg tctcgggcgc cgcggggtcg atcccgggga
      661 tccggataag gactggaaga ctgatcgaca tggcgaaacc tcacgtcgtg gtgatcggtg
      721 gcgggatcgc gggcctggcc gcggccgcgt tcctcgggga cgcggcggtg cgggtgaccg
      781 tgctggaggc gtccggacgc ttcggcggga aactgcgtgg cggcgaggtc ggcggcgtcc
      841 gggtcgacct cggcgccgag tcgatgctcg cccgccgccc cgaagcggtc gaactggccc
      901 gccgggtcgg cctcggcgaa cggctcgaac cgcccaccac cgccaaggcc gcgatctgga
      961 cccgcgaggg cctgcgcccg ctgcccggcg gccaactcat gggcgtaccg ggcgacctgg
     1021 acgcgctcag ggcctccggc gtgctcaccc ccgagggcct ggagcgggcc ttgcgcgagc
     1081 agcccgccga gatcggcgag gacgccccga tcggccgcta catcgccgac cggctcggcc
     1141 gcgaggtcgt cgaccggctg gtcgaaccgc tgctcggcgg cgtgtacgcc gggcacgccg
     1201 acgagatctc gatccgcgcc gccgtcccgc aactgctcca actcgccggg cagggcggca
     1261 gcctgaccac cgccatcggt gagctcggcc gccgcgccac cgccaccggc ccggtcttcc
     1321 agggcctgga cggcgggctc ggcaccctgc ccggggccgt cgccgcgcac gaccgcgcgc
     1381 acggcgccga actgctcacc gaccaccccg tcgacgccct gcgccgcacc cccgacggct
     1441 ggcgggtcac cgccgccgga cgcgaactcg ccgccgacgc cgtcgtgctg gccgtccccg
     1501 ccccgcaggc cgccgccctg ctcgccgccg acgcccccgg cgccgccgcc gaactccggc
     1561 aggtcgagta cgcgtccatg gccctggtca ccctcgcctt ccgccgcgcc gacctcgcgg
     1621 gcagcccgct caccggcagc ggcttcctcg tccctccggt cgaccggcgc agcatcaagg
     1681 cctccacctt ctccagcaac aagtggggct ggctggagcg ttccgccccc gacgccttcc
     1741 tgctgcgcac ctcgctcggc cgccaccgcg aggacgccgc gctcgacctg gacgacgccg
     1801 aactcgtcgc ccgctcgctc gccgacctgc gcgccgccgt cggcctgcgc gccaccccgt
     1861 acgaccacgc cgtcacccgc tggcgggccg ggctcccgca gtaccccgtc gggcacctcg
     1921 accgggtcgc ccggatccgc acccgcctcg acgccctcgg cgcgatcgcg gtctgcggcg
     1981 ccgcgtacga cggcgtcggc atccccgccg tcgtcgccgg tgcccggcgg gccgccgagc
     2041 acctcgcccc cgccaccccc ggcaccggaa ccacccgcac ggaagggaca atgggcccat
     2101 gaccgaaagc actgagcagc ccgtgaagaa gaaggcccgc gacctcaacc aggtcatccg
     2161 ctacaccatg tggtcggtgt tcaagctcaa ggacgccctc cccgaggacc gcaccgcgct
     2221 cgccgccgag gtcgacgcac tcttcgagca gctcaccgcc aaggacgtcg tcatccgcgg
     2281 cacctacgac gtgtccggcc tgcgcgccga cgccgacgtc atggtctggt ggcacgccga
     2341 gagctccgac gacctgcagg aggcgtacaa ccgcttccgc cgcaccgggc tcggccgcca
     2401 cctggagccg gtctggtcga acatggcgct gcaccgcccc gccgagttca acaagtcgca
     2461 catcccggcg ttcctcgccg acgagaaccc ccgcgagtac gtctgcgtct accccttcgt
     2521 ccgctcctac gagtggtacc tgctcccgga cgaggagcgc cgggcgatgc tcgccgagca
     2581 cggcatgatg gcccgcggct acccggacgt ccgggccaac accgtcgcct ccttcgcgct
     2641 cggcgactac gagtggctgc tcgccttcga ggccgacgaa ctgcaccgca tcgtcgacct
     2701 gatgcgcgac ctgcgcccct cccgggcccg cctgcacgtc cgcgaggagg tccccttctt
     2761 caccggccgc cgcaagccga tcgccgacct gctggccggt ctgtactgag cgagcaccga
     2821 atcaggggcc cgcggtaaga acggcgggac gtgacatcag gggcccggta tgaacggcgg
     2881 gacgtgacat caggggcccg gggaacggcg ggtcgatgct gctgacggtc ggagcccatc
     2941 gaaggtccgt gctgctgcgt gtctttgacc gaacccgaag cagatgcgct gggcggcagt
     3001 gcaccgccag cagccacggg ctcgccgttc cccgagcccc tgatttcgct cccctaggag
     3061 tcgagggccg cgcgcagccg ggggtcccgg acggcggcgg ggccggagat ggcgacggcg
     3121 gtgagcagtt cgtcgtcggt ggcgggggcg tgccggcagg ggagggcgcg cagcagggtc
     3181 tggccggcgg gggcggccca cgcggtgtag gcgtggacgt cgatccgggc catcagcagg
     3241 gtgccgccgg tgaggaccag cgggagcgcg taccaggcgg ccgcggtacg ggcggcggtg
     3301 ccgtcggggg cggtgagcag gacggtcagc agcagcatcg ccgccgcccc gacgagcgcg
     3361 tagcggacct gccggaccgc ccgcagggcg tggtcccgga cgacggccgg ggtggccagc
     3421 cccgccgagg tcagccggtc gccgatgtcg cggacggtgg ggtgcgcggc cagcacggtc
     3481 ttcaactggg cggtgcggca ctggccctcg gggccgatcg cgccgatcag cgcccgctcc
     3541 aggcggccgc ggccccgcgg gtcgacgacg gtggtccagc cggtgtgggc caggtgcagg
     3601 cggccgaccc cggccatccg gaccagggcc aggtcgacca cccgggccgg gccgccggcc
     3661 aggtacgcgg tctcgtacag gccgacgccc gcggggcggt ggaccgggtg cggggtgccc
     3721 ccggcgaggg cgtccgcgat cgaggcgacc cgtaccagcc ggaggcagga gagcaccgcg
     3781 gcggcacagg acgggatcag cagcaacacc cacatgcttt ttgttctagt cctgtgcgcg
     3841 ccgggaacgg aacacgtacc cgggccgcga taccgaactg tgactcgtcg ggcggctcag
     3901 gaactgccgc acccgccgcc cccgcaactg gacccgcagg agctcgacga cccgcaggag
     3961 tgcgaggagg agccgcacga gtgcgaggag gaggagccgc aggagtgcga ggacgacccg
     4021 caggaatggc cgcccgaatg cccgcccgag tggtggccgg aggagccgca ggacgaggcg
     4081 ggcgtcgacc cgcaccacgc cgccgagccc gcgcccgcgc cggccgcgaa ctccggcgtg
     4141 ccgtccgcgc ccgagccgcg ggaggagcgc ccggagcggg tggcggcggc cgggccctcc
     4201 aacgccgcgc gcagcgcccg gagttccggc ttgcgcagcg acccggcgcc gtccagggcg
     4261 aacgcgccga ccaccgccgc gtgcgcccgg tccacctcgt gcggccgcca ggggcccttg
     4321 ccgcggacca gttgcagctg gcgcaggccg gccggggtga tccggcgcct cgggacgcgg
     4381 gaggcgacca ggacgacgac cgtgagcagg acgagcggga tgaacgggat gaacgggggc
     4441 gcggtggtgt cccagccggc cgagcccgcc cactggacgg tggagaccgt gccgaggacg
     4501 atcgcgacca gcagcgccag caccaccagc aggcgggccc gccgggcgct gcgcagcgcg
     4561 ccggggtggc gcatcagccc gcgggcggcc agcccgtcgc cgatccgctg cacctccggg
     4621 ctgcccgtca cggaggcgcg cagcaccgac aggtcgcgcc cggcccggcc gccgacggcg
     4681 gtgatcagcg tccgctcgac cgggtcggac gtccggtcgt cggtgacggt gacccggccg
     4741 gcccgggaga tcgccacccg gccctcgcgc tccatccgga ccagcgcggt gtcgaccgcc
     4801 cgggccgggc cgccggccag gaaggcgacg tccagcagcg gctggccgcg accgggcagg
     4861 ccctgcggat cggtgacccg gcggctgcgc cggttgcgga cggcggccag ggcgagggtg
     4921 aacgcgacga gcgcgaacgc caggacgaac cggaagttcc acatcccccg tgattccttt
     4981 ctctcggtgc tgcggggtgg ttacgggtgg gcgcgggcgg cgcgggcgcg gcgcggcagc
     5041 aggcggctcc accgggaggc ggcggggtgc agcaggcggc gcagcgcggg gtgccagtgg
     5101 gcgtccgggg cggtgtccag ggcccattcg gcgaaggccc gggcgtcggc gcggtagccg
     5161 ccggtgagcg gccggccggc gcagtagcgg gcgaacagcg gggcgaagtc cgggccgagc
     5221 agctcgggga gctccggggc caggtggccg acggtctccc ggcgcttggc gagcaggccc
     5281 tcggcctgca cccgcagctg ctccgggtcg aagccgccgg gggcgggggc gccggccacc
     5341 agggcggcca gcagcttctc ctggcggtgc gccaggtgga cgcgggcgag ggcgagttcg
     5401 gcgtcctcgc ggtcgcggat caggtactcg tgctgctcag acacgggcgg tctccctgcg
     5461 gtggaggtgc tggacgcggc ggatggcgtc gagttcggcg gacagttcgg cgggcggcgg
     5521 gtacgcgccg tcccgctcca gcagcacccc gggcgggtcg gtgcgggcgc agagcccggc
     5581 cagcacctcc agcacgggcg cggtgacggg gtgggtgtgg gtgtcgtgcc agacgccgtc
     5641 gcgctcgacg cccccggcga cgtgcacgta ggcgagggcc tccagcggca gccggtccag
     5701 ctcggcggcc gggtcgacgc cgaggttgac ccggttggtg tgcaggttgg cgacgtccac
     5761 cagcaggcgg acgccggtgc gctcgaccag ctcggccagg aactgcgcct ccgtcaactc
     5821 gtctcccggc caggcgagtt gggcggcgat gttctccagg gcgagcggca ccggcagctc
     5881 ccgctgggcg atccgcacgt tcgcggccac cacccgcagc gcctcccggg tgcgcggcac
     5941 cggcagcagg tggccggcct ccaggccgcc cgcccggacg aacgccaggt gctcgctgac
     6001 cagcggcgcc ccgagcgact cggcgacggc ggccagccgg gccagccggg ccgggtcggg
     6061 cgcggtggcg tcccccagac cgagcgagac gccgtgcggg acgaccgtga ccccgcgggc
     6121 gcgcaactcg gccagcgagg gcggcaggtg gccggggcag acgttctcgg cgaccacctc
     6181 cacccagtcc agacccggca gccgttccac cacggtgtcg atctccgggc gccagccgat
     6241 gccggtgccc aatcgcggaa tatcggacga agacatattc caccccctct tcacgggctt
     6301 ttccggccgc gccgaccccc gttgccgagg gtgtatccat gccgcaccga tgccaagcgg
     6361 gacaccggaa actcagagct gaacttgagg tttcaccgcg gccggacgaa cttccgcccg
     6421 ggtgggggga aattcagcgg cgcacggacg aacgactggc taacgtgaca tgcctatgac
     6481 ttccacgccg taccgcatcc gcccgttcgc ccccgccgac gccgccgacg ccgccgcggc
     6541 ctaccgggcc ggccgcccgc acctggtcac cacgcccgag gtggtcgcct ggcaggccga
     6601 actgccgcac taccacctgc tggtggccga acgggccgac cgggtggtcg gcaccgcccg
     6661 gttcgggccg gtgcccgaga gcaccacctc ccaccagggc ttcctcaaca tcagcgtgct
     6721 ccccgagcac cgcggcaccg gcgccggcac cgccctgctc gccgccgccg aacgggacct
     6781 cgccgccctc ggcgtccggg acgcgcacag ctgggtcgac gacacccccg aggccctcgg
     6841 cttcgccgcc gcccgcggct accggcgcag cggccgggcc ggccgcttca gcggcatcga
     6901 cctcaccgcc ccgctgcccc cgctgccgcc caccccgccg ggcgtcgaac tgcgcaccgc
     6961 cgccgacttc ctggacgacc cgtacccgat ctacctggtc gacatcgacg gcacccgcgc
     7021 cgaacccggc gacctggtcc tcgacgacca gccgtacgag ggctggctcg ccgagatctg
     7081 gcagcgcccc gaccaggacc acaccctcac cgcggtcgtc ctggcggacg gtgaaccggt
     7141 cgccttctcc gccgcccaga ccgacggcca ccgcacctac tggtccgcct tcaccgccac
     7201 ccgctccagc caccgcggcc gcggcctggc cgggctcgcc aaggcccact cgctgcaccg
     7261 ggccaaggaa cgcggcttca ccgccgcgta tacccagaac gacgcgggca acgccccgat
     7321 gctcgccatc aacgcccggc tgggctaccg cgagtgcgcg gcggagtgga agcacacccg
     7381 ggcgatctgc gagtagccgc gcggcggggc gggccccggc gcgcgctccg cggcccgcgc
     7441 tcacgcgacg tccacgccgt ggtcgcgggc caggccggcc aggccgtcgg cccagccctg
     7501 gccgaccgcg cggagcttcc agccgtcggc cgggtggcgg tacacctcgg cgaccagcat
     7561 cgcggagacc gccgggtcgg ccggcgggcg gaacacccag cgaccggtgc cggaggcgag
     7621 ttcgaccgcc gggtcgacca ggtccgcgca ggtgccgtcg ccgtccgcgt ccaggttcac
     7681 cgagatcgcc acccgccgca ccgcgtccgg gagccggccg gtcagcacgg tcgcgctgcg
     7741 gtcgtcgggc cgcagcgcca cggcgccgcc ctccgcgacg ggctggtggt agaagacgaa
     7801 gtcctcgtcc cgccggacct tcccgtccgc gtcgagcagc agcagcgtca ggtcggccgc
     7861 cgccccgccg gcccggaacc ggacgcgcac ctcgccgtcg ggcagcacca ggttctgacc
     7921 cggcgccaac tccgtcgcgg ggacggcggg ggcggcgggg acggcgggga gggtcggggt
     7981 ggcgggggtg gtcggagcgg ccggggcgct ccgctgggcc gggaccaccg ccggggccgc
     8041 cggggccgcc gggccgagcc gtcccggcag gccctgctcg cggagcagct ccagcagcat
     8101 cgggccgtcc accagggtga gcggcttgtt ctcgacgtgc cgccgggagc tggggccgaa
     8161 gcccgccgtg gtcaccagga tgccgcggtt ggcgccctgg tggcgcatgg tcgactccag
     8221 gtcgcggacg gcgctcggcg ggacggtctt ccggtagcgc ttggcctgga tgacgatctt
     8281 cccgccggtg atcgggtccg ggtcctcggc cagcacgtcc acgccctcgt cgccgctgcg
     8341 cgcggtggtg ctggtgcgca ggccccggcg gcggaacaac tcggcgatca gctcctcgaa
     8401 ctccagcggg tccatctcga acaggtcggg gtccgcgccg tccgggccgt ccgggccgtc
     8461 cgcgccgtcc gggccgttcg cgccttctgc gccgtccggg tcctgcacgc cgggggccgg
     8521 ggcggcggcc gggacttcgg cgacggtgtc ggcccgctcc ggccgggcgg cgatccgccc
     8581 gccgagggcc tcgacgaagc agtccagcgg ggccacccgg tccagcgcga gcgcctcgaa
     8641 gacggcgcgg tcggcctcga cggcgagcag gcagcgctcg ccctcccgcc cggtcgccgg
     8701 gtcggtggcg accaccacgc cgttcaggcc caccgaggcc agcagccccc cggtatccgc
     8761 ccggaacacc tccgccagca cccgcagcgc gcactgcgcc agcatctcgc ggtacagctc
     8821 cctgcgctgg gtggcgggcc gggccacctc gtcctcgcgg tcgtccgagc ggacgtagcg
     8881 gtaccggccc accgccggca ccacctcgta cggcggcagc tcccagcgca ccaccaggcg
     8941 gcgggccggg gcgtcccagg cggtgtcgcc gtcggcgggg aagccgtccg gccagtcctc
     9001 gcgccagtcg accaccgcct cgaagtactc ggcgaccgcc gccgcctcgc ccgcccgcag
     9061 ccgcgcggcc aactgcccgg cctgcgcgga ctgcccggcc agcagccggt gccgctcggc
     9121 cgcccacgcg tcgtactggg cgcggtagtc ggcgagttgg cgctgccgct gctgctcggc
     9181 cgcccaggcg gcctgccagt cgtgctcgaa ggcggcccgg gcccgcgcgg agtgctcctc
     9241 ccactgccgg cgcgcccccg ggtcgtacgc caggtggccc gccgggggcg gcaccaggta
     9301 ccaggcctgg tccggcatcg gcaccggcac gcccagcgga ccgggatcga acggcggcac
     9361 cgccgggcgg cccccggcgg ccagcgagaa cccggggccg gccagccccg ccgccagcac
     9421 cccgcgcaac tcggccaccc gcgcgtccag ttcggcgctg cgccgggcgg cgtccgactc
     9481 ccgcccctgc tggtacgcct tcagcgcctc gcgctcgccg cgcgccgccg tccgctgcgc
     9541 ctcccgccgg gcgcgctcgt tctcgcgctg ccgggcgtcc gccgccctgc gctgcgcctc
     9601 cgcgcggcgg tgctgctcgc gctgcgcctg agccagtacc gaccacaccc cggaagaccg
     9661 ccgccgcgcc acgcccgccc cacccacgtc caagaccgtt cggaagacga tcagtcttcc
     9721 acgccgaccg gtacccggat caaccgcggc cgtaccctgg ccggaaaccc cggacgcgcc
     9781 cggttccggg acgcgagacg gaaggccctc gatgagccac acgacggtga acggcgccac
     9841 cctgcaccac gacgagtccg gcccggcgga cggcaccccc gtcgtcctgc tccacggcca
     9901 cccgttcgac cggacgctgt gggaaccgca ggtccgcgcg ctcgccgcgg ccgggtaccg
     9961 ggtgatcacc cccgacctgc gcggctacgg gcggagcgag gtgacgccgg gcaaggtgct
    10021 gctcgccgac ttcgcggacg acaccgcggc cctgctcgac cggctgggcg tcgagcgcgc
    10081 ggtggtcggc ggcgtctcga tgggcggcca gatcgccatg gagttccacc tccgccaccc
    10141 cgggcggctg ctggcgctcg tcctgtccga cagctcggcg cccgccgaga ccgaggaggg
    10201 caaggaggtc cgcaaccgga tggccgaccg gctgctcgcc gagggcatgg cgggctacgc
    10261 ggacgaggtg atcgacaaga tgctcgcccc ctacaacgtc accgccctcc ccgacacggc
    10321 cgcccgggtc ctggccatga tgcgcgccac cgacccgcgc ggcgccgccg ccgccctgcg
    10381 cggccgggcc gagcgccccg actaccgcga gaccctgcgc accgtcaccg tccccaccct
    10441 gctgctggtc ggcgccgacg acgtctacac ccccgtcgcc gactccgagg cgatccgggc
    10501 cctcgtcccg cacgccgacc tgaccgtcgt ccccgacgcc ggacacctcc ccggcgccga
    10561 acagcccgaa cgcttcaacg ccgccctgct ggacttcctg cgcacccggc tctcctgacc
    10621 gccggcccct cccgacaacc cgcgagggcc ctccccggca ggcggggagg gccctcgtgc
    10681 ggtcgcggcc gctactcggc gggcttcagc gtcagcgaga tcgagttgat gcagtaccgc
    10741 tggtcggtcg gggtgtcgta cccctcgccc tcgaagacgt gcccgaggtg gctgccgcac
    10801 ttcttgcagc gcacctcgac ccggcgcatg ccgagactgc tgtcctccag gtactccacc
    10861 gagtcgccgg ccagcggcga gtagtacgac ggccagccgc agtgcgagtc gaacttcgtc
    10921 tcgctgctga acagctcggc gccgcacgcc cggcaggcgt agacgccgac cgtcttggtg
    10981 tcggtgtact cgccgacgaa cgggcgctcg gtgccggcct cgcggagcac gtggtactcc
    11041 tccggggtca gctcggcgcg ccactcggcg tcggacttct ggacctcgta gctcacggcg
    11101 actctccagc tcggacggga acgtcagaca gcaggtgcaa cgtccgccag ggcggccaga
    11161 atttccgggc ccaggttggt gacgtcgccc gcacccatgg tcagcaccag gtcgccgggg
    11221 gcggccagcg cggccagcag cgccggggcg gctgcgaagt cgtgctcggc ccggac
//
