LOCUS       NZ_CP054919            10912 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Kitasatospora sp. NA04385 chromosome, complete genome.
ACCESSION   NZ_CP054919
VERSION     NZ_CP054919.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora sp. NA04385
  ORGANISM  Kitasatospora sp. NA04385
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 8400742)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Genome mining for natural products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8400742)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Institute of Functional Biomolecules, State
            Key Laboratory of Pharmaceutical Biotechnology, Nanjing University,
            No.163 Xianlin Avenue, Nanjing, Jiangsu 210046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013364235.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 08:11:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,532
            CDSs (total)                      :: 7,423
            Genes (coding)                    :: 7,206
            CDSs (with protein)               :: 7,206
            Genes (RNA)                       :: 109
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 81 of 218
            Pseudo Genes (incomplete)         :: 153 of 218
            Pseudo Genes (internal stop)      :: 9 of 218
            Pseudo Genes (multiple problems)  :: 25 of 218
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:10:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4677722
            Orig. end                         :: 4688634
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10912
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="17"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10912
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10912
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5912](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5912)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(567..581)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            1360..1815
                     /locus_tag="HUT16_RS20985"
                     /old_locus_tag="HUT16_20985"
     CDS             1360..1815
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /locus_tag="HUT16_RS20985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_20985"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_176189655.1"
                     /transl_table=11
                     /translation="MSTPQHTPQPRRIEVVEGIFDDGLDGAGITWTPYVQPGRDAVDVH
                     WLYTSEETGPDGAEAYVAQFRPGAHGDLHRHLGFELIVVLDGELVNDNGDRYGRGTLVV
                     ERPDSVHRVTSPLGCRLLVVREKRTLPLHPGEEADTGLALDGARSAA"
     misc_feature    1406..1421
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.86"
                     /tool="antismash"
     gene            1849..3156
                     /locus_tag="HUT16_RS20990"
                     /old_locus_tag="HUT16_20990"
     CDS             1849..3156
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="HUT16_RS20990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_20990"
                     /product="MFS transporter"
                     /protein_id="WP_176189656.1"
                     /transl_table=11
                     /translation="MTDQLASPLRPPAPVDAVPVGPLVGQSGKVHTPRFKWGILGIGIA
                     AQAAFSAAFQGIPTSGSLLQDAYRLTPSQLGLVLAAVTCGAFVTDVFWGLMSDRIGERK
                     VLITGLTGTTLALAAAALFLSPGGGAVPSYTALAVVLFMAGCLGGSVNGASGRAVMGWF
                     PVHQRGFAISLRVAAVPAGGAIGAAVLPPLAMNGGFRWVFAFLTAFSLLATVAVVIWLD
                     EPPLAKTKAQGGTAAPLAPNPLKRWDIWRIAITAFLLDLPQFTVLTFGSVFLHTVKHVS
                     LGTIAVLLVVVQVLSAVSRVWGGRWTDRRGGRHRRTMVTVYSWIIAGGFTAVALLESGP
                     AWLVAALLVVSGILACGWHGVHYAEIATMAGAERSGTALGLENTLVFAGAFVTPLLIPA
                     LLSASSWPVVMLLIGALPAVLSALIMPREPKLPARA"
     gene            3336..3947
                     /locus_tag="HUT16_RS20995"
                     /old_locus_tag="HUT16_20995"
     CDS             3336..3947
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574798.1"
                     /locus_tag="HUT16_RS20995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_20995"
                     /product="ABATE domain-containing protein"
                     /protein_id="WP_176189657.1"
                     /transl_table=11
                     /translation="MSASTDLRFDTGRICLDLLATVGGNLSSSPVERLDGVARLDAWLH
                     GAGVVPVDEPLDLDAEALDGFLALRARLHRIVHAELDGTGPTDDDLAALNRTAAAGPLA
                     LRLDRTPGAGLHCRTVTPPDLADLLGAVAQDAVRLFGSPDRALLRECEGPTCDLVYVDA
                     SRGRRRRWCSASACANRHYVAAHRARRSEAGPPSPSGEPA"
     gene            complement(4030..4929)
                     /locus_tag="HUT16_RS21000"
                     /old_locus_tag="HUT16_21000"
     CDS             complement(4030..4929)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="HUT16_RS21000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_21000"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_176189658.1"
                     /transl_table=11
                     /translation="METRWLQTFTLAARTGSNSAAAERLGVARSTVTGHIQGLERALGA
                     RLFDRNLPGSPLTRSGAALLEHAETILDDMERARLAVARAAGSGDPAALHLGATPSVCA
                     YQLPSFVKMMNRFLPELSLEIETATPGRLREQVLSGGTSVVLVNRTKEPWRAGNDGDDP
                     VHSRVLWEEDALLVGTRDAAARPRRVLLTGRDCVYRELTETDVLERLPDAEPMQVGSLE
                     GVKSAVLAGLGVGLLPEVAVRPWLAKGHLVEMPLETGHVVVTEVLWNRRTCPAGVSAHL
                     RRLRPAPSRRGTPTLQAV"
     gene            complement(5001..5912)
                     /locus_tag="HUT16_RS21005"
                     /old_locus_tag="HUT16_21005"
     CDS             complement(5001..5912)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="HUT16_RS21005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_21005"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_176189659.1"
                     /sec_met_domain="AfsA (E-value: 1.9e-20, bitscore: 71.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKASTLPGALVHKRDTAEILLTDWRELGPDRFELSARWPASHPFY
                     RTADGGHSPLLLVETVRQAIPLLSHAAYGAPLDHRQSWCDLSLTVDPAALAAADSRHPV
                     ELRVSCSDVVRRAGRLVSLRMAVQVLADGVPLARVHTGFVNHPPAIYRRLRGDRADPER
                     ARAGAPPLLPPLPPARVGREHHTDVVLAPARRDDRTQLRVDLSHPVLFDHPVDHVPGML
                     LLEAAQQAAHAACRSRPGLVVGMDSVFARYAEFDAVCDVTAAPLDPHPEDRPGRRRFVV
                     SATQDRTCVFSTVVTLDDAPGR"
     misc_feature    complement(5962..5977)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     misc_feature    complement(5986..6000)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 15.7"
                     /tool="antismash"
     gene            6138..6764
                     /locus_tag="HUT16_RS21010"
                     /old_locus_tag="HUT16_21010"
     CDS             6138..6764
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264896.1"
                     /locus_tag="HUT16_RS21010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_21010"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_176189660.1"
                     /transl_table=11
                     /translation="MHKPKQERAIQTRAAILRAAAEVFDECGYTGASISKIIARAGLTQ
                     GAVYFHFASKEEMARAVMLEQANNLRFPDGVPGLQHLVDLTMFLAVQLQEDSLLRAGVR
                     LAVEQGEIFGNRDDTAYRDWVERFAADLYAATGRSELLPDVVVPDLAQFLVGAYSGTQL
                     FSNISTGRADLPQRLVVMWRYLLPGIATPEARARMVVRPWGAARP"
     gene            6893..7897
                     /locus_tag="HUT16_RS21015"
                     /old_locus_tag="HUT16_21015"
     CDS             6893..7897
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (1-139). E-value: 8.2e-11.
                     Score: 33.4. Matches aSDomain:
                     nrpspksdomains_HUT16_RS21015_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="HUT16_RS21015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_21015"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_254897914.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.2e-12, bitscore:
                     45.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGATGFLGSAVVSQLALQPVDLRLGMRLPRPAGDKGLSGAEAET
                     GGEGPPRAGGEPLARVHRVRADLDDRASLDALCADADVVLHLASYVGPDRDRAEAVNHR
                     GAERLAAAARATARPHCRILHLSTCAVYGAGPHRGAAVGEVVPDPVSPASRTRLAGERA
                     TRAAGATVLRAALVTGPGERWVVPALRELVHRVPAHWDGGRALLSMIDAADLGRLVAAL
                     ALEPAAAAPGAVLHAAHPAPVRNRDLLRALADLGLLPPLPDGADRPWSWCLDRLARTPG
                     SATERQLALLGQDHWYRSEDAWRLARTDPGPGPLARLAAAAPWYRAHLALPGG"
     aSDomain        6896..7309
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUT16_RS21015_NAD_binding_4.1"
                     /evalue="8.20E-11"
                     /label="HUT16_RS21015_NAD_binding_4.1"
                     /locus_tag="HUT16_RS21015"
                     /protein_end="139"
                     /protein_start="1"
                     /score="33.4"
                     /tool="antismash"
                     /translation="TGATGFLGSAVVSQLALQPVDLRLGMRLPRPAGDKGLSGAEAETG
                     GEGPPRAGGEPLARVHRVRADLDDRASLDALCADADVVLHLASYVGPDRDRAEAVNHRG
                     AERLAAAARATARPHCRILHLSTCAVYGAGPHRG"
     misc_feature    6959..6961
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7970..7987)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.94"
                     /tool="antismash"
     gene            8939..9709
                     /locus_tag="HUT16_RS21020"
                     /old_locus_tag="HUT16_21020"
     CDS             8939..9709
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137142.1"
                     /locus_tag="HUT16_RS21020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_21020"
                     /product="hypothetical protein"
                     /protein_id="WP_176189662.1"
                     /transl_table=11
                     /translation="MADLASGFDSLPEAQTQAQAQAEHAAKAGPEVGPEPGSEAGPGPG
                     PEAGPEAEPAVEAPAVDEEIRAAMGLLYRVIQPEPRLRIGEVEALALAPLVAEWQRRGG
                     TPEDLARALLPGLPSLVHSAPGMLRSRLERKMPPAPDVPPEPARVASSGSGRSPGLSGP
                     FARSGLSARSGSPGSSGMPVVPRPRASYAECAKCHDPVPSPGICGPCAGLAPRPVAVGG
                     GAGVVRVGAQRARAALRAARDVLPISHCLPAVTG"
     misc_feature    complement(10076..10091)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.57"
                     /tool="antismash"
     misc_feature    complement(10202..10216)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
ORIGIN
        1 cttcgagcgc ggcggggtgc gccacctggt ggtccgcagc cgggacgtgg accggttcgc
       61 ggcggtcgac gcggccgtcc gggtcgcggt cggcgggccg gtgccgggct ggcacgacca
      121 cgcggacgcc cgccgcacgc ccggcgcgtt caccccggac gccgccaccc cggccgggga
      181 cgtggccttc ggctacttca cctcggggac gacctccgcc ccgaagctgg tcctgcacac
      241 ccacgcctcg tacccggtcg ggcacctctc ctcgctgtac ttccacgggc tgctgccggg
      301 cgaccggcac ctgaacgtct cggcaccggg ctgggccaag cactcctggt cgagcttctt
      361 cgtcccgttc acggcgcagg ccacgctggt cgtcgcggcc gacgagggcc tcggcccgga
      421 ggggctgccg gggctgctgg cggagcggga ggtcaccagc gtctgcgcgc cgccctcgta
      481 ctgggcggcg gtggtgccgc acctgggcac ggccgtcccg cggctgcggg aggccaccag
      541 cgcgggggag ccgctgcccg atccggtggc cgacgcggtc gcggcggcct ggggcgtgac
      601 ggtgcgcgac ggctacggcc agaccgagac gaccgcgctg atcggcacca cccccggcac
      661 gccccgcacc cccggctggc tgggccggcc gctgcccggc tgggacctcg tcctggacga
      721 gggccacctg tgcgtggacc tgaccggcgc cccggtcggg atgatggccg gctacgacga
      781 ccccgagcac acccgccggg tgctcggcgg cgaccgctac cgcaccggcg acgtggccga
      841 ggcgggcccg gacgggctgc tgcgggtcct cggccgggac gacgacgtgt tcaagtccgg
      901 cggccaccgc gtctcgccgt acgagctgga ggcggtgctg cgcacccacc cggcggtccg
      961 ggacgccgcg gtcgtcccgg tgccgcacgc cgggctgggc cgggccgcgc acgcggtggt
     1021 cgagccggag ccgggccgtc cggcggtgac ggccgaggaa ctgctggcgc acgtggacgc
     1081 ccgggtgggc gcggcggtgc gggtccacag cgtggagttc accgaccgcc tgccgcgcac
     1141 cgtctccggc aaggtccgcc gggcggcgct ggcccgctga gccgaccggt cgtttcggtc
     1201 ggccgtcgta gcggtgaacc cggcggtccc gcaagccggt tcacgattcc cgaacactcc
     1261 ggtcctccgg gccggcgcgg ttgcgccggc gccggaagac cgccactcta gggagtaagg
     1321 gttttgaacc cttactcgac ctcatcggag gagagcaccg tgtccacgcc ccagcacacc
     1381 ccccagcccc gccggatcga ggtcgtcgaa gggatcttcg acgacgggct cgacggcgcc
     1441 gggatcacct ggaccccgta cgtccagccc ggccgcgacg ccgtcgacgt ccactggctc
     1501 tacacctcgg aggagaccgg ccccgacggc gccgaggcgt acgtcgccca gttccgcccc
     1561 ggagcccacg gcgacctgca ccgccacctc ggcttcgagc tgatcgtggt gctggacggc
     1621 gagctggtga acgacaacgg cgaccgctac ggccgcggca cgctcgtcgt cgaacgcccc
     1681 gacagcgtgc accgggtcac cagcccgctg ggctgccggc tcctggtcgt ccgcgagaag
     1741 cgcaccctgc cgctgcaccc cggcgaggag gccgacaccg gcctggccct cgacggcgcg
     1801 cggtccgccg cctgaccgac ccctccacca cgcctccagg agagcaccgt gaccgaccaa
     1861 ctcgcctccc ccctccggcc gcccgccccc gtcgacgccg tgcccgtcgg gccgctggtc
     1921 ggccagtccg gcaaggtgca caccccgcgc ttcaagtggg gcatcctcgg catcgggatc
     1981 gccgcccagg ccgcgttcag cgccgccttc cagggcatcc ccacctccgg ctcgctgctc
     2041 caggacgcct accggctcac cccctcccag ctcggcctgg tgctggccgc ggtcacctgc
     2101 ggcgcgttcg tcaccgacgt gttctggggg ctgatgtcgg accggatcgg cgagcgcaag
     2161 gtgctgatca ccggcctgac cggcaccacc ctcgcgctcg ccgccgccgc cctcttcctc
     2221 agccccggcg gcggcgccgt cccctcctac acggcgctgg cggtggtcct cttcatggcc
     2281 ggctgcctcg gcggctccgt caacggggcc tccggccggg cggtgatggg ctggttcccg
     2341 gtccaccagc gcggcttcgc catcagcctc cgggtggccg ccgtcccggc cggcggggcg
     2401 atcggcgccg ccgtgctgcc cccgctcgcg atgaacggcg gcttccgctg ggtgttcgcc
     2461 ttcctgacgg ccttctcgct gctggccacc gtcgccgtgg tgatctggct ggacgagccc
     2521 ccgctggcca agaccaaggc ccagggcggc accgccgccc cgctggcgcc caacccgctc
     2581 aagcgctggg acatctggcg gatcgccatc accgccttcc tgctcgacct gccgcagttc
     2641 accgtcctga ccttcggctc ggtcttcctg cacaccgtca agcacgtctc gctcggcacg
     2701 atcgccgtcc tgctggtcgt cgtgcaggtc ctcagcgcgg tctcccgggt ctggggcggc
     2761 cgctggaccg accgccgcgg cggacgccac cgccgcacca tggtcaccgt ctacagctgg
     2821 atcatcgccg ggggcttcac cgccgtcgcc ctcctggagt ccggcccggc ctggctggtc
     2881 gccgccctgc tggtggtctc cggcatcctc gcctgcggct ggcacggcgt gcactacgcc
     2941 gagatcgcca ccatggcggg ggccgaacgc tcgggcaccg cactggggtt ggagaacacg
     3001 ctggtcttcg cgggcgcctt cgtcaccccg ctgctgatcc cggccctgct gtccgcctcg
     3061 tcctggccgg tggtgatgct gctgatcggc gcgctcccgg ccgtgctgtc ggcgctcatc
     3121 atgccgcgcg agccgaagct cccggcgcgg gcctgaccgg gctccgcgcc cggcgccccg
     3181 tccgtggcac catcggcccg tgggggtcgg tggtgccggt ggtgccggtg cgcccggccc
     3241 tggcctcggc ctcggctccg gctccggctc cggccccggc ctcggccccg gccccggaac
     3301 ggcaactgga actggaacag gaaggagagc gtcccgtgag tgccagcacc gacctgcgct
     3361 tcgacaccgg ccggatctgc ctcgacctgc tggccaccgt cggcggcaac ctctcgtcga
     3421 gcccggtcga acggctcgac ggcgtcgccc gcctggacgc ctggctgcac ggcgcgggcg
     3481 tggtgcccgt cgacgaaccg ctcgacctgg acgcggaagc gctggacggc ttcctggccc
     3541 tgcgcgcccg cctccaccgg atcgtccacg ccgaactcga cggcaccggc cccaccgacg
     3601 acgacctggc cgcgctcaac cgcaccgccg cggccggccc gctcgccctc cgcctggacc
     3661 gcacccccgg ggcgggcctg cactgccgga cggtcacccc gcccgacctc gccgacctgc
     3721 tcggcgcggt cgcccaggac gccgtccggc tgttcggctc gccggaccgc gcgctgctgc
     3781 gcgagtgcga gggcccgacc tgcgacctgg tctacgtgga cgcctcgcgc ggccgacgcc
     3841 gccgctggtg ctccgcctcg gcctgcgcca accggcacta cgtggccgcc caccgggccc
     3901 gccggtcgga ggcgggcccc ccgtccccgt ccggggagcc cgcctagccg cgcaccccgc
     3961 accccggagc cccgtccgct ctcgtcggac ggggctccgg ggtgcgcggc gtaggacgcc
     4021 gggggtggtt cagacggcct ggagggtcgg ggtgccgcgg cgggacggcg cggggcgcag
     4081 tcgtctcagg tgggccgaga cgccggcggg gcaggtgcgg cggttccaga gcacctcggt
     4141 gacgaccacg tgcccggtct ccagcggcat ctccaccagg tggcccttgg ccagccaggg
     4201 ccgcaccgcg acctccggca gcagcccgac gccgagaccg gccagcaccg ccgacttcac
     4261 gccctccagg ctgcccacct gcatcggctc ggcgtccggc agccgctcca gcacgtcggt
     4321 ctcggtcaac tcccggtaga cgcagtcccg tccggtcagc agcacgcggc ggggccgggc
     4381 cgcggcgtcg cgggtgccga ccagcagcgc gtcctcctcc cacagcaccc ggctgtggac
     4441 cgggtcgtcg ccgtcgttcc cggcccgcca gggctccttg gtccggttga ccagcaccac
     4501 gctcgtcccg ccggagagca cctgctcgcg caggcgtccg ggggtggcgg tctcgatctc
     4561 caggctcagt tccggcagga agcggttcat catcttcacg aagctcggca gctggtaggc
     4621 gcagaccgac ggggtcgctc ccaggtgcag ggctgcgggg tcgccgctcc cggcggcccg
     4681 ggccaccgcg agccgggcgc gctccatgtc gtcgaggatc gtctcggcgt gctccagcag
     4741 ggccgccccg gaccgggtga gcgggctccc cggcaggttg cggtcgaaca gccgggcgcc
     4801 cagggcccgt tccaggccct ggatgtgtcc ggtgacggtc gagcgggcga cgccgagtcg
     4861 ttcggcggcg gcgctgttgc tcccggtgcg ggcggccagc gtgaaggtct gcagccagcg
     4921 ggtttccatg tccgtcctct cccttgctcg tctgttgcct tgctcgcctg ttcccctgct
     4981 cgtctgtcgg tgcctgcccg tcagcggccg ggcgcgtcgt ccagggtgac gacggtggag
     5041 aacacgcagg tccggtcctg cgtggcggag accacgaacc tgcgccgccc gggccggtcc
     5101 tccgggtgcg ggtcgagcgg ggccgcggtg acgtcgcaga ccgcgtcgaa ctcggcgtac
     5161 cgggcgaaga cgctgtccat gccgacgacc agcccgggcc gcgagcggca ggcggcgtgc
     5221 gcggcctgct gggcggcctc cagcagcagc atgccgggga cgtggtcgac cgggtggtcg
     5281 aagagcaccg ggtggctgag gtcgacgcgc agctgggtgc ggtcgtcccg gcgggccggg
     5341 gccagcacca cgtcggtgtg gtgctcgcgg ccgacccggg ccggcgggag cggcgggagc
     5401 agcggcggcg ccccggcgcg ggcccgctcg gggtcggcgc ggtcgccgcg cagccgccgg
     5461 tagatcgcgg gcgggtggtt gacgaagccg gtgtgcacgc gggccagcgg caccccgtcc
     5521 gccaggacct gcaccgccat ccgcagggac accagccggc cggcccggcg caccacgtcc
     5581 gagcaggaca cccgcagttc gacggggtgc cgggagtcgg ccgcggccag cgcggcgggg
     5641 tcgacggtca gcgacaggtc gcaccaggac tggcggtggt ccagcggcgc gccgtacgcg
     5701 gcgtgcgaga gcagcgggat ggcctggcgc accgtctcga cgaggagcag cggggagtgg
     5761 ccgccgtcgg cggtgcggta gaaagggtgc gaggcgggcc agcgggcgga gagttcgaac
     5821 cggtccggtc cgagctcgcg ccagtcggtg agcaggatct ccgccgtgtc gcgcttgtgg
     5881 accagcgcac ccggcagcgt gcttgctttc actcttcccc ccgagaagac gcggggcgac
     5941 gcccggtggg gcgccgcggc tctggaaaaa tagtgaccgt cctgattttt agcacgagaa
     6001 ggtgagcatc agaagggcac tcgggatcat ttgaactcct ctatacccaa gggttgtccc
     6061 gccacgggca agggctgtgg gagcgtgcag gtgccggtta tgatggcgaa cgttcggatt
     6121 tttttggagg tgggatcttg cacaagccca agcaggagcg ggcgatccag acgcgggcgg
     6181 cgatccttcg cgcggcagcc gaggtcttcg acgagtgcgg ctacacgggg gcgagcatca
     6241 gcaagatcat cgcccgggcc ggactcaccc agggcgccgt gtacttccac ttcgcctcga
     6301 aggaggagat ggcccgggcg gtcatgctgg agcaggccaa caacctgcgc ttccccgacg
     6361 gcgtgcccgg cctccagcac ctggtcgacc tcaccatgtt cctcgccgtc cagctccagg
     6421 aggactcgct gctccgggcc ggcgtccggc tcgccgtcga acagggcgag atattcggca
     6481 accgggacga caccgcgtac cgcgactggg tcgaacgctt cgccgccgac ctgtacgcgg
     6541 cgaccggccg cagcgaactg ctgcccgacg tggtggtgcc cgacctggcc cagttcctgg
     6601 tcggcgccta cagcggcacc cagctcttct ccaacatctc caccggccgg gccgacctgc
     6661 cgcagcgcct ggtcgtgatg tggcgctacc tgctgcccgg catcgccacc cccgaggccc
     6721 gcgcgcgcat ggtggtccgg ccgtgggggg cggcgcggcc gtgagacggg acgccggggc
     6781 gcgggacgcc gggcgttcgc gggttgccgg ggtgcggggc gctgagcgtt catcgccggt
     6841 tgtggtggca cgggatgccg tgagacgggg cgccggacgt ccgttggtcg cggtgaccgg
     6901 tgccacgggc ttcctcggtt cggccgtcgt ctctcaactc gccctccaac cggtcgattt
     6961 acgcctcggg atgcgcttgc cccgtccggc cggggacaag ggtctgtccg gggctgaggc
     7021 tgagaccggg ggtgagggcc cgcctcgggc cgggggcgag ccgctcgcgc gcgtgcaccg
     7081 cgtccgcgcg gacctggacg accgggcgag cctggacgcg ctgtgcgccg acgcggacgt
     7141 ggtgctgcac ctcgcctcct acgtcggccc ggaccgggac cgcgccgagg ccgtcaacca
     7201 ccgcggcgcc gaacggctcg cggccgcggc ccgcgccacc gcccggccgc actgccggat
     7261 cctgcacctg tcgacctgcg ccgtgtacgg agccgggccg caccggggcg ccgccgtggg
     7321 cgaggtcgtc cccgaccccg tctcgcccgc gagccgcacc cggctggccg gtgagcgggc
     7381 cacccgcgcc gccggggcga cggtgctgcg cgccgcactg gtcaccggcc cgggcgaacg
     7441 ctgggtggtg cccgccctgc gcgaactcgt ccaccgggta ccggcccact gggacggcgg
     7501 ccgcgccctg ctctccatga tcgacgccgc ggacctgggc cgactcgtcg ccgccctggc
     7561 cctcgaaccc gccgcggccg cccccggcgc ggtcctgcac gccgcgcacc ccgccccggt
     7621 gcgcaaccgc gacctgctcc gggccctggc cgacctcggc ctgctgccgc ccctcccgga
     7681 cggtgccgac cggccctggt cgtggtgcct ggaccggctc gcccgcaccc ccggctccgc
     7741 caccgaacgg caactcgccc tgctcggcca ggaccactgg taccgcagcg aggacgcctg
     7801 gcgactcgcc cgcaccgacc ccggccccgg cccgctcgcc cggctggccg ccgccgcacc
     7861 ctggtaccgc gcccacctcg cactcccggg cggctgactc gcgagcgttg gcctcgcggg
     7921 cgccggtctc gcggtcgtcg gtctcgtgcc gggggagctg atggggtcga ttacaccagg
     7981 tgaacatttc tggatggtga tgcgtcatca aatgaccgat gctgaatcat cgttgacgca
     8041 gcactgaccc tgtttgcgca ctgtgcgcgg gggagtgatg ggtgtgttac gttccgtact
     8101 cggaccaccg aaaaattgac cccggcggtg ctaccaacac cccggggccc ggcactagga
     8161 gttcacgctc cccatgcaca tccatcgtag cgggcgcccg cgcggcttcg accccttccc
     8221 gatgctccga cccgagcgtt ccggcctcag caagggcgcg tacggactcg ccgtccagat
     8281 cctcaccagc cccaccggct gccaggagga cggccgacag ctggccaccc acggcccgga
     8341 cggccgggcg ggcatcgcca acatgctgcg cgagctgaag gccaccaggt tctactgggt
     8401 catcaccttc cggctcccgg acggtcggat cgtcagccag tcccacctct tcgacactcc
     8461 gcagcacgtc gcaccggatc ccgtcctccc gggccccggc gaaggcggac ccggccgacc
     8521 tgacgcccta ttgaaaaccc gggagggaaa accctccctc ccgggcgccg aaccaccgcc
     8581 tgcggcgaag gccgcggcgg gctccgggcc ggagcccgat ccgaagttgg agcccgggcc
     8641 gggggccggg ccgagggccg ggtcgagggc agaagccggg tccgggtgcg ggtgcgggcc
     8701 gggggccgcg ccgggggccg ggtccggggc cggtctcggc ccggagcctg aggccgtggc
     8761 cgatctcgcg tcggggtccg actcgttgcc agaggcccag gcccaggccc aggccgagca
     8821 tgcggcggag gccgggccct acgggtcggg ggccgggtgc gggtacgggc cgggggccgg
     8881 gtcgggggcc gggtccgggt acgggtcggg ggcaggtctc ggcccggagc ctgaggccgt
     8941 ggccgatctc gcgtcggggt tcgactcgtt gccagaggcc cagactcagg cccaggccca
     9001 ggccgagcat gcggcgaagg ccgggccgga agtcgggccc gaacccggat cggaagctgg
     9061 gcccggaccc ggaccggaag ccgggcccga ggccgaaccg gctgtcgagg caccggcggt
     9121 ggacgaggag atccgggcgg cgatgggcct gctgtaccgg gtgattcagc cggagccccg
     9181 gctgcggatc ggcgaggtgg aagcgctcgc gctggccccg ctggtggcgg agtggcagcg
     9241 gcgcggcggc acgccggagg atctggcgcg ggccctgctg cccggcctgc cgtcgctggt
     9301 ccactcggcg ccgggcatgc tgcgctccag gctggagcgc aagatgccgc ccgcgccgga
     9361 cgtaccgccg gagcccgccc gggttgcgtc gtccggttcg ggcaggtcgc ccgggctgtc
     9421 tgggccgttt gcccggtccg ggctgtctgc gcggtccggg tcgcccgggt cgtccgggat
     9481 gcccgtggtg cccaggccga gggcgagcta cgccgagtgc gccaagtgcc atgacccggt
     9541 gccgagtccg ggcatctgtg ggccctgtgc gggactggcg cccaggcccg tcgcggtcgg
     9601 cggtggcgcg ggcgtcgtcc gggtcggcgc gcagcgggcc agggccgccc tgcgcgccgc
     9661 gcgggacgta ctgccgatca gccactgcct gcccgccgtc accgggtagt ccccgggcgg
     9721 cagttggggc actgtgggcc tccgggcctc cgggcctccg ggcctccggg cctccgggcc
     9781 tccgggtgga cggtggtggt ggggcctccg ggcctccggg cctccgcgtg ggcggcggtg
     9841 gtggggcctc atccggcctg gccgtgggag cccggcttca gggtcgtgcg gagggcggct
     9901 gggggcgagg cgatgtggtc agcgcggcga gctcgctctc gatcgcgggg cgggcggcgg
     9961 tggccggtcg ccccgcggcc tcgggcgtga gcgtgcggcg gaggatgggc gagcggcagt
    10021 cctgggggtg cacgacccgc tcgcagctcc gacaccagtg cgctggcatc ggtgcgttga
    10081 cacctgtgtt ccggcatcgg tgcgtcgaca tcgatgcgtc ggcacctgtg ttctgacatc
    10141 ggtgtgccga catcgatgcg tcggcatccg tgtcccgaca tcggcgtgcc gacaccgatg
    10201 catcgacacc cgtgttccga catcgatgag tcggcatcga tgcgccgaca tctgtgttcc
    10261 gacatcgatg cactgacacc gacccaccgg cccaccgaca ccgacccgcc gacccgccga
    10321 cccgccgacc cgccagcgtc gttcagggcg cggccaagcg gtggcgcagg ccgagcagga
    10381 agacgtccag cccggtggtg aaccaggcgt cgtagtagga gtgttggggc gccgctccgg
    10441 tggggtcgcc atcgctgtcg gacgggctgc cgggcggggt gccggtctgg gccagggcgt
    10501 ggccgaggac gtagacggcc acggtctgga cggccgtgac ggcctcctcg tcggacactc
    10561 cggcggtggt gaaggcccgt agcagtgggg cgatcaggcg gcggccgtgg gtctcgtcca
    10621 ccgggtgggt ggcgagcagc gggacggcgc gggggtgggc gaggagcatt cggcggtacg
    10681 cggtggcgaa ggcgtgtagc gcggtgggcc agtcggtgtc cggcggcagg tcgagccgga
    10741 cttcggcggc cagccggttg accaccgagg cgagtacggc gtcccggttg ggcaggtggc
    10801 gatagagcga catggcggcc acgcccagtt cggtgcccag gcggcgcatg gtgagcgcct
    10861 ccgtcccctc ggcgtcgatc acgccgatcg cggcctcggt gacggcggcg gc
//
