LOCUS       NZ_CP054919            10801 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Kitasatospora sp. NA04385 chromosome, complete genome.
ACCESSION   NZ_CP054919
VERSION     NZ_CP054919.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora sp. NA04385
  ORGANISM  Kitasatospora sp. NA04385
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 8400742)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Genome mining for natural products
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8400742)
  AUTHORS   Zhang,B., Shi,J. and Ge,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2020) Institute of Functional Biomolecules, State
            Key Laboratory of Pharmaceutical Biotechnology, Nanjing University,
            No.163 Xianlin Avenue, Nanjing, Jiangsu 210046, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_013364235.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 08:11:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,532
            CDSs (total)                      :: 7,423
            Genes (coding)                    :: 7,206
            CDSs (with protein)               :: 7,206
            Genes (RNA)                       :: 109
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 218
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 218
            Pseudo Genes (frameshifted)       :: 81 of 218
            Pseudo Genes (incomplete)         :: 153 of 218
            Pseudo Genes (internal stop)      :: 9 of 218
            Pseudo Genes (multiple problems)  :: 25 of 218
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:10:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1867568
            Orig. end                         :: 1878369
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP054919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5801)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    686..700
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.97"
                     /tool="antismash"
     gene            759..2081
                     /locus_tag="HUT16_RS08090"
                     /old_locus_tag="HUT16_08090"
     CDS             759..2081
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135058.1"
                     /locus_tag="HUT16_RS08090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08090"
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="WP_176186861.1"
                     /transl_table=11
                     /translation="MVAQAPAPAVRAPDRPVRWTRPATWPREVLALAAYWVASRVLMVG
                     LIRSGRAEPAVEVHQLYRGWYRVLRTGTFPVDDVTWQYPPGAALPMLAPGALPLLSYLQ
                     AFVLLMLLADAAVQVALLAAGRGPGRSVWGAWAWALALPLLLGLPYGRYDLAATALAVG
                     ALLLFPARPRLGGALAGIGALVKVWPALTVLGTPRGRTTRQAWTAAAVSALALLLVFAA
                     FFDHTFDFLHAQRGRGVEIESLGGSVLQLARQFGWSGRVEQHYGSFEFLGRYVHRLSQL
                     SLLLTAAAFGWLLLWRVRARRWTAATPYDAALSAVLLFTVTSRVISPQYLVWLIGLVAV
                     CLTVEGTTQRPVAFLLLPAVGVTTVDYPLFFDSMQAGGWWPTGVILLRNGLLLAASCWS
                     AALLWRATVTPPPAARPVAGEPGGPRAEPRADPDQDAVPAA"
     gene            2110..2493
                     /locus_tag="HUT16_RS08095"
                     /old_locus_tag="HUT16_08095"
     CDS             2110..2493
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009342530.1"
                     /locus_tag="HUT16_RS08095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08095"
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="WP_176186863.1"
                     /transl_table=11
                     /translation="MADTALDRLAAGKYLLITSYKKDGTGVPTPVWVVRDGDALGAWTT
                     AHSWKVKRIRRRADVLVGPCDVRGNPRGEALPARAEIADAETTERYRRLLARKYGLVGR
                     LTLLGSRLRRGRTGTVGLTLRLT"
     gene            complement(2516..3298)
                     /locus_tag="HUT16_RS08100"
                     /old_locus_tag="HUT16_08100"
     CDS             complement(2516..3298)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135060.1"
                     /locus_tag="HUT16_RS08100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08100"
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_176186865.1"
                     /transl_table=11
                     /translation="MRQYRAMVAVGDSFTEGMCDDVLPDGHFRGWADRVAAALAAEAGS
                     AGFRYANLAVRGKLIGQIRAEQVGPAAATDADLVTLAGGLNDVLRPGCDVAQVERLLGE
                     CVRGLLAEGRTVVLFTSTDPTRRMAGSRRLLPAILRMKAYVEGLVREHPGQVVLVDLFS
                     APVFDDRRLWAEDRLHLSPEGHRRVALAVLEALGRPADRDWRAPLAEPDTRTRGRKARE
                     DVRWLGTHVGPWIGRRLRGRSSGDGRPAKRPDLLPYGG"
     gene            complement(3399..4652)
                     /locus_tag="HUT16_RS08105"
                     /old_locus_tag="HUT16_08105"
     CDS             complement(3399..4652)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135061.1"
                     /locus_tag="HUT16_RS08105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08105"
                     /product="cytochrome P450"
                     /protein_id="WP_176186867.1"
                     /sec_met_domain="p450 (E-value: 2.3e-17, bitscore: 61.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAPQPEPHPSFHGCPVLDPTASAPHAEAAALRERGPAALVELPG
                     GVRAWSVTRHAVIQALTTDPRVSRDYRRHWPGAAQVPEGWVLGSVAFQDSFFNQYGAEH
                     RAARGRIAPTFLPRRAERMRPQVQETADRLVDELGALAPGERADLREALARPLTMTVIC
                     DLFGVPEKMRETLGELVDTVLDSSLDETAALAAQTELYGRLGELVEYKRARPASDLTSD
                     LLLSDRPGEQPLTEHQLLGTLLTMIAAGFETAVNLITSAALELLTHPEESARLRAGAIG
                     WADVIEETLRLDGPVMHLPLRYAVEDIDLGEGVLIRAGDPIIIGFAAAGRDPERHPDRP
                     DAFDPSRPDKGHLAFGHGPHFCLGAPLARLEAEIALRTLFERLPDLALAEPGREPARLP
                     SMIVSGPAALEVVPRPAA"
     misc_feature    complement(4645..4656)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     misc_feature    4759..4774
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.07"
                     /tool="antismash"
     gene            complement(5001..5801)
                     /locus_tag="HUT16_RS08110"
                     /old_locus_tag="HUT16_08110"
     CDS             complement(5001..5801)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894062.1"
                     /locus_tag="HUT16_RS08110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08110"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_176186869.1"
                     /sec_met_domain="Linocin_M18 (E-value: 6e-92, bitscore:
                     306.1, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRDLAPVSAAAWAEIEEEARRTFRLHVAGRRVVDLAGPDGP
                     GLAAVGTGHLADIAAPANGIRARARESLQVVELRVPFTVTRAAVDDVERGSKDSDWDPV
                     KDAARTIALAEDRAVFDGYQAAGITGIRDGSSNHPVALPADVREYPNAVSRALTALRLA
                     GVGGPYSLLLGADTFTAVNETSDHGYPVHNPLARLLDGEIIWAPAVEGAVLLSARGGDF
                     ELHLGEDLSIGYLSHDDTTVHLYLQESLTFQLHTPEAAVTLTSA"
     gene            complement(5798..6823)
                     /locus_tag="HUT16_RS08115"
                     /old_locus_tag="HUT16_08115"
     CDS             complement(5798..6823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135065.1"
                     /locus_tag="HUT16_RS08115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08115"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_176186871.1"
                     /transl_table=11
                     /translation="MPQPQPVLATLTGAAVFLVLGIEDGGEQTVRDLLPDLGGLARSVG
                     TRAPREEVSLVLGIGSDAWDRLFAGPRPADLHPFVELAGPRHLAPATPGDLLLHIRAER
                     MDLCFELATLTSRRLGDRVTVLDETHGFRYFDCRDLIGFVDGTENPTGRDAAEAVLIGG
                     EDPAFAGGSYVIVQKYLHDMAGWNAIGTEHQERVIGRTKLDNVELDDAAKPADSHVALN
                     TVTGPDGEDRDIWRLNMPFGSFAEGGECGTYFIGYARDPGVTEEMLRNMFLGRGPAAHD
                     RLLDFSTAVTGTFFHVPSADFLDDPPPAPGAPAPAAEAVPVPAVRDGSLHIGSLKRSTA
                     P"
     gene            complement(7015..7467)
                     /locus_tag="HUT16_RS08120"
                     /old_locus_tag="HUT16_08120"
     CDS             complement(7015..7467)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135066.1"
                     /locus_tag="HUT16_RS08120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08120"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_254897704.1"
                     /transl_table=11
                     /translation="MAQREFGRLLQGLASEMNLLGHDFAAASGLHATDVQALLAVLRAA
                     DPPGITPGGLRAELGLTSGAVTAVLDRLERAGHVHRTRDEADRRQVHVHYHPDAARIAG
                     EWFAPVAECTDRVRAEFAPEQVATVVRFLTRMTEELAELRLARRAE"
     gene            complement(7527..8447)
                     /locus_tag="HUT16_RS08125"
                     /old_locus_tag="HUT16_08125"
     CDS             complement(7527..8447)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135067.1"
                     /locus_tag="HUT16_RS08125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08125"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_176186875.1"
                     /transl_table=11
                     /translation="MPEELPEVTASTGEVARRLGVSPTTVRSWERRYGIGPAERAEGRH
                     RRWTVRDVAVLEAMCRLTARGTPPAEAARLAPALAGRPAEPAAVEAPAGQVRPEVRGLA
                     RAARRLDAPEVARLLHASVGRLGAVAAWTDVMTPALRAAGRSWAAGGGEHHVEVEHLLS
                     WHVSSALRRIAEPPAAAAGPPVLLAAMPGEQHTLPLEAVAAALSERGLPFRIFGAALPV
                     EALAAAVRRTGPAAVLLWSQTRATADPAPARAVAATAWGTRGARVRPALLAAGPGWQRA
                     TRPPGALLPRDLPGALDLLARALGS"
     gene            complement(8573..9475)
                     /locus_tag="HUT16_RS08130"
                     /old_locus_tag="HUT16_08130"
     CDS             complement(8573..9475)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658073.1"
                     /locus_tag="HUT16_RS08130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08130"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_176186876.1"
                     /transl_table=11
                     /translation="MALDVHTRDLRYFLAVAEELHFTRAAERLHVSQPALSRQVRALEQ
                     RLGTELFRRDRHRVTLTAAGHTLLPHAREVLDGWTRGADALAAAKAEQRSTLTVGMSTS
                     PARGGLLPAVRSRLGATHPQVTTRLRQVSWDDPTAGLADGSTDVAFVWLPLPDPHRYDW
                     TVVAEEPRRIALPAAHPLAARPAVDFADLLDEPFLALPPDAGPLRDHWLALDARAGRPP
                     RIGAEVAGVEETYEALIAGLGVCLVADGNAPLIAHAGVTTRPVTGIGPSHYALAWRRED
                     ARHPLVRAYAEACRATVGG"
     gene            9572..10402
                     /locus_tag="HUT16_RS08135"
                     /old_locus_tag="HUT16_08135"
     CDS             9572..10402
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (3-161). E-value: 2.5e-11. Score:
                     35.8. Matches aSDomain:
                     nrpspksdomains_HUT16_RS08135_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019072711.1"
                     /locus_tag="HUT16_RS08135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HUT16_08135"
                     /product="oxidoreductase"
                     /protein_id="WP_176186878.1"
                     /sec_met_domain="adh_short (E-value: 1.6e-30, bitscore:
                     105.2, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNRVWLVTGAGSGFGRALTLAAVAAGDVVVGAARRPESLDDLVAA
                     HPDQVEALGLDVTDTAAVGAAVERVVARHGRIDVLVNNAGRTHVGAVEETTDEELRALF
                     DVHVFGPAALVRAVLPHMRERRAGAVVQMSSVGGQLSFAGFGAYSATKFALEGMSEALA
                     DEVREYGVRVLVVEPGAFRTSLFAASRAGVSADGGRYAAVAATRAAVAGGDGAQPGDPD
                     KAAALILAALEAERPPLRLVLGEDAVDGVLGHLEQVREDVLAWEERSRATAFDA"
     aSDomain        9581..10054
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HUT16_RS08135_PKS_KR.1"
                     /evalue="2.50E-11"
                     /label="HUT16_RS08135_PKS_KR.1"
                     /locus_tag="HUT16_RS08135"
                     /protein_end="161"
                     /protein_start="3"
                     /score="35.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VWLVTGAGSGFGRALTLAAVAAGDVVVGAARRPESLDDLVAAHPD
                     QVEALGLDVTDTAAVGAAVERVVARHGRIDVLVNNAGRTHVGAVEETTDEELRALFDVH
                     VFGPAALVRAVLPHMRERRAGAVVQMSSVGGQLSFAGFGAYSATKFALEGMSEA"
     CDS_motif       9680..9793
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_HUT16_RS08135_0001"
                     /evalue="5.90E-03"
                     /label="C2_DCL_024-062"
                     /locus_tag="HUT16_RS08135"
                     /protein_end="74"
                     /protein_start="36"
                     /score="8.9"
                     /tool="antismash"
                     /translation="ESLDDLVAAHPDQVEALGLDVTDTAAVGAAVERVVARH"
ORIGIN
        1 agcgagccga ccaggtcggc gtcctcgccg aagcgctcga ccaggatggc gcgggcgccc
       61 tccagcgcgg cgggcgcgtc ggcgacctgc tcgttgacgt accgggcggc gagggcggac
      121 gggtcctggc ccgggtcggc gagcagggtg tcggcgagcg gttcgaggcc ggcctcgcgg
      181 gcgatctgcg ccttggtgcg ccgcttgggc ttgtacggga ggtagatgtc ctccagccgg
      241 gccttggagt cggcggcgag gacctgggcg cgcagcgcgt cgtccagctt cccctgggcc
      301 tcgatggact ccaggacggc ggtgcggcgc tcctccagct cgcgcaggta ccgcagccgc
      361 tcctccaggg tgcgcagctg ggtgtcgtcg agggcgccgg tggcctcctt gcggtaccgg
      421 gcgatgaagg gcacggtcgc tccgccgtcg agcaggccga cggcggcctg cacctggttc
      481 tcgcggacgc cgagttcttc ggcgatcttg cgttcgacgg gcatggtcac ggtgccggtc
      541 accccttcat gacggttgcg cggtgtcgcc cgagcattct ggcaggtccg accgacggga
      601 gtccggtgga ccgggtggcc gcccggggcg tggcgcgtcc cggggacgtc cggggacacg
      661 cgccgagcga cccggagcct gcccgatttg tctgatatga gagacggacg accttttcga
      721 gcccgccccc gggacggcat cagagcgcac tggaggacat ggtggcccag gcccccgccc
      781 cggccgtccg ggctcccgac cggccggtcc gctggacccg ccccgccacc tggccccgcg
      841 aggtgctcgc cctcgccgcg tactgggtcg ccagccgggt gctgatggtc ggtctgatcc
      901 gctccggccg ggccgagccc gccgtcgagg tgcaccagct gtaccggggc tggtaccggg
      961 tcctgcgcac cggcaccttc ccggtcgacg acgtcacctg gcagtacccg cccggcgcgg
     1021 cgctgccgat gctcgcgccg ggcgcgctgc cgctgctgtc ctacctccag gcgttcgtgc
     1081 tgctgatgct gctggccgac gccgccgtgc aggtcgcgct gctggcggcc ggccggggcc
     1141 cggggcgctc ggtgtggggc gcctgggcct gggcgctggc cctgccgctg ctgctcggcc
     1201 tgccctacgg ccgctacgac ctggcggcca ccgcgctcgc ggtcggcgcg ctgctgctgt
     1261 tcccggcccg gccccggctg ggcggggcgc tggccgggat cggcgcgctg gtgaaggtgt
     1321 ggccggcgct gaccgtcctg ggcaccccgc gcggccgcac cacccgccag gcctggacgg
     1381 ccgccgccgt gtcggcgctc gccctgctgc tggtgttcgc cgcgttcttc gaccacacct
     1441 tcgacttcct gcacgcccag cgcggccggg gcgtggagat cgagtcgctg ggcggcagcg
     1501 tcctccagct ggcccggcag ttcggctggt ccggccgggt cgagcagcac tacggctcgt
     1561 tcgagttcct cggccggtac gtgcaccgcc tctcgcagct gtcgctgctg ctgaccgccg
     1621 ccgcgttcgg ctggctgctg ctgtggcggg tgcgggcccg gcgctggacg gccgccaccc
     1681 cgtacgacgc ggcgctgagc gcggtgctgc tgttcaccgt caccagccgg gtgatctccc
     1741 cgcagtacct ggtgtggctg atcggcctgg tggcggtctg cctgaccgtg gagggcacca
     1801 cgcagcggcc ggtggcgttc ctgctgctgc ccgcggtcgg cgtgaccacc gtcgactacc
     1861 cgctgttctt cgactcgatg caggccggcg ggtggtggcc gaccggggtc atcctgctcc
     1921 gcaacgggct gctgctggcc gcctcctgct ggtcggcggc cctgctgtgg cgggccaccg
     1981 tgacgccgcc gcccgcggcc cggccggtcg ccggggagcc cggcggaccg cgcgccgaac
     2041 cgcgcgccga cccggaccag gacgccgtgc cggcggcctg acggccggtc ggcgccgtac
     2101 actcccccaa tggccgacac cgcgctggac cggctcgccg ccggcaagta cctgctgatc
     2161 accagttaca agaaggacgg caccggcgtg cccacccccg tctgggtggt ccgggacggc
     2221 gacgcgctcg gggcgtggac caccgcgcac tcctggaagg tcaagcggat ccggcggcgc
     2281 gccgacgtcc tggtcggccc gtgcgacgtg cgcggcaacc cgcgcggcga ggcgctgccc
     2341 gcgcgcgccg agatcgccga cgccgagacc accgagcgct accggcggct gctcgcccgc
     2401 aagtacgggc tggtcgggcg gctcaccctg ctcggcagcc ggctgcgccg cggccggacg
     2461 ggcacggtcg gcctcaccct gcggctcacc taggcgcggg cccgcccggc cccgctcagc
     2521 ccccgtacgg cagcaggtcc gggcgcttgg ccgggcggcc gtccccggag gagcgcccgc
     2581 gcagtcggcg gccgatccac gggccgacgt gggtgcccag ccagcggacg tcctcgcgcg
     2641 ccttccggcc gcgggtgcgg gtgtccggct cggccagcgg cgcccgccag tcccggtccg
     2701 ccgggcggcc gagcgcctcc agcaccgcca gcgcgacccg gcggtggccc tccggggaca
     2761 ggtgcagccg gtcctccgcc cagagccggc ggtcgtcgaa caccggcgcc gagaacaggt
     2821 cgaccagcac cacctggccc gggtgctcgc gcaccaggcc ctccacgtac gccttcatcc
     2881 gcaggatcgc gggtagcagc cggcggcttc cggccatccg gcgggtcggg tcggtggagg
     2941 tgaacagcac cacggtgcgg ccctcggcga gcaggccgcg gacgcactcg ccgagcagcc
     3001 gctcgacctg cgcgacgtcg cagcccgggc gcagcacgtc gttcaggccg cccgccagcg
     3061 tcaccaggtc ggcgtcggtg gcggcggccg ggccgacctg ctcggcgcgg atctggccga
     3121 tcagcttccc gcgcaccgcc aggttggcgt accggaagcc ggccgacccg gcctcggcgg
     3181 ccagtgcggc cgcgacccgg tccgcccacc cgcggaagtg cccgtcgggc aggacgtcgt
     3241 cgcacatccc ctccgtgaac gagtcgccca ccgccaccat cgcccggtac tgccgcacac
     3301 cgcctcctgg gtcgttcccg accgcccacc ctaccgaggc gtgaccggtg cgttcgggcg
     3361 gccgccgggc cgggtgctca ggccgccggg cggggtgctc aggccgccgg gcggggcacc
     3421 acctccagcg ccgccggacc gctgacgatc atcgagggca gccgtgcggg ctcccggccg
     3481 ggctcggcca gcgcgaggtc gggcagccgc tcgaacagcg tgcgcagcgc gatctcggcc
     3541 tccagccggg cgagcggcgc gcccaggcag aagtgcgggc cgtggccgaa cgccaggtgg
     3601 cccttgtcgg gacgggaggg gtcgaacgcg tccgggcggt cggggtggcg ctccgggtcg
     3661 cgtccggcgg cggcgaaccc gatgatgatc ggatcgcccg cgcggatcag cacgccctcg
     3721 ccgaggtcga tgtcctcgac cgcgtaccgc agcgggaggt gcatgaccgg gccgtccagc
     3781 cgcagcgtct cctcgatcac gtccgcccag ccgatcgcgc ccgcccgcag ccgggccgac
     3841 tcctcggggt gggtgagcag ttcgagggcc gcgctggtga tcaggttgac ggcggtctcg
     3901 aacccggcgg cgatcatcgt cagcagcgtg ccgagcaact ggtgctcggt cagcggctgt
     3961 tcgccgggac ggtcggacag cagcaggtcg ctggtcaggt cggacgccgg acgggcccgc
     4021 ttgtactcca ccaactcgcc caggcgtccg tagagttcgg tctgcgcggc cagcgccgcg
     4081 gtctcgtcga gggaggagtc cagcaccgtg tcgaccagct cgcccagcgt ctcgcgcatc
     4141 ttctccggga caccgaacag gtcgcagatc accgtcatcg tcagcggccg cgccagggcc
     4201 tcccgcaggt cggcgcgctc cccgggggcg agcgcgccga gttcgtccac cagccggtcg
     4261 gcggtctcct gcacctgcgg gcgcatccgc tccgcccggc gcggcaggaa ggtcggcgcg
     4321 atccggcccc gcgcggcgcg gtgctcggcc ccgtactggt tgaagaagct gtcctggaac
     4381 gcgaccgacc ccagcaccca gccctccggc acctgcgccg cccccggcca gtgccggcgg
     4441 tagtcgcggg agacccgcgg atcggtggtc agcgcctgga tgaccgcgtg ccgggtcacc
     4501 gaccaggccc gcacgcctcc cggcagttcg accagcgcgg cggggccccg ctcccgcagc
     4561 gcggcggcct cggcgtgcgg tgcgctcgcc gtcgggtcga gcacgggaca gccgtggaag
     4621 gacggatggg gttcgggctg cggagcgttc atgtgcgggc ctccttcggg tggggtacca
     4681 cgtccagggc cgccgggccg ttgacgatca tcgacgggac gcgggcgggc tcccggccgg
     4741 gttcggccgg atcggggaaa ggacacctgg aaacatcgga accgggcacg gcgcgctccc
     4801 ctcaccggaa tccccgcacg agtcctcaca tcgtgcggcc ccgcgccccg ccccggaagg
     4861 cttcccccgg cattgtccgg attccgcccc cggctctccc aactcccctt ccagtagcca
     4921 cagttggccc ggaaaaaccc acccgccccg gccgggcggg ccggagcggg gcggggtggg
     4981 cgggggtgcg ggcggccggg tcaggcggag gtgagggtga cggccgcctc cggggtgtgg
     5041 agctggaagg tgagggattc ctggaggtag aggtggacgg tggtgtcgtc gtgggagagg
     5101 tagccgatgg agaggtcctc gccgaggtgg agttcgaagt cgccgccgcg ggcggagagc
     5161 aggacggcgc cctcgacggc gggggcccag atgatctcgc cgtcgaggag gcgggcgagg
     5221 gggttgtgga cggggtagcc gtggtcggag gtctcgttga cggcggtgaa ggtgtcggcg
     5281 ccgagcagca gcgagtaggg gccgccgaca ccggccaggc gcagcgcggt gagggcgcgg
     5341 ctgacggcgt tggggtactc gcggacgtcg gcggggaggg cgacggggtg gttggaggag
     5401 ccgtcgcgga tgccggtgat gcccgcggcc tggtagccgt cgaagacggc gcggtcctcg
     5461 gcgagggcga tggtgcgggc ggcgtccttg accgggtccc agtcggagtc cttggagccg
     5521 cgctcgacgt cgtcgacggc ggcccgggtg acggtgaagg ggacgcggag ctcgacgacc
     5581 tggagggact cccgggcgcg ggcccggatg ccgttggcgg gggcggcgat gtcggcgagg
     5641 tgcccggtgc cgacggcggc gagtccgggg ccgtcggggc cggccaggtc gaccacccgg
     5701 cggccggcga cgtgcaggcg gaaggtgcgc cgggcctcct cctcgatctc ggcccaggcg
     5761 gcggcggaga cgggcgccag gtcgcggtgc aggttgttca cggtgcggtg ctccttttca
     5821 ggctgccgat gtggaggctg ccgtcccgga ccgccgggac gggcacggct tcggcggccg
     5881 gggcgggcgc gccgggggcg ggtggcgggt cgtccaggaa gtccgcgctg gggacgtgga
     5941 agaaggtgcc ggtgacggcg gtggagaagt ccagcagccg gtcgtgcgcg gcggggccgc
     6001 ggccgaggaa catgttgcgc agcatctcct cggtgacccc ggggtcgcgg gcgtacccga
     6061 tgaagtaggt gccgcactcg ccgccctcgg cgaaggagcc gaacggcatg ttcagccgcc
     6121 agatgtcgcg gtcctcgccg tccgggccgg tgacggtgtt cagcgcgacg tgcgagtcgg
     6181 cgggcttggc ggcgtcgtcg agttcgacgt tgtcgagctt ggtgcgaccg atcacgcgtt
     6241 cctggtgctc ggtgccgatc gcgttccaac ccgccatgtc gtgcaggtac ttctgcacga
     6301 tcacgtagct gccgccggcg aacgccgggt cctcgccgcc gatcaggacg gcctcggcgg
     6361 cgtcccgtcc ggtggggttc tcggtgccgt cgacgaagcc gatcaggtcg cggcagtcga
     6421 agtaccggaa gccgtgcgtc tcgtcgagca cggtgacccg gtcgccgagc cggcggctgg
     6481 tcagggtggc cagttcgaag cagaggtcca tgcgttcggc gcggatgtgc agcagcaggt
     6541 cgccgggggt ggcgggggcg aggtgccggg ggccggcgag ttcgacgaag gggtgcaggt
     6601 cggccgggcg ggggccggcg aacaggcggt cccaggcgtc ggagccgatg ccgagcacca
     6661 ggctgacctc ctcgcgcggg gcccgggtgc cgacgctgcg ggccaggccg ccgaggtcgg
     6721 gcagcaggtc gcggacggtc tgctcgccgc cgtcctcgat cccgagcacc aggaacacgg
     6781 cggccccggt cagcgtggcg aggaccggtt ggggctgcgg catggcgggt gctcccaggg
     6841 ggctgccgga cgttcggacg cccgccgacc ataccgggca aaaggttcga gaacccgcga
     6901 tggcgcccga atcgggcgtg tccggcggga cggttccggg gcccgtccgg gaacccgccc
     6961 ggggcctgtc cgggaacccg tccggggccc gccgccggcc gcccggtccg gcggctactc
     7021 ggcccgtctg gccagccgca gttcggccag ttcctcggtc atccgggtga ggaagcggac
     7081 cacggtggcg acctgctccg gcgcgaactc ggcccgcacc cggtcggtgc actcggcgac
     7141 gggggcgaac cactcgccgg cgatccgggc cgcgtccggg tggtagtgga cgtgcacctg
     7201 gcgccggtcc gcctcgtccc tggtccggtg gacgtggccg gcccgctcca gccggtccag
     7261 caccgcggtc accgcgccgg aggtcagccc gagttcggcc cgcagcccgc cgggcgtgat
     7321 cccgggcggg tcggccgccc gcagcaccgc cagcagcgcc tggacgtcgg tggcgtgcag
     7381 ccccgaggcc gccgcgaagt cgtgccccag caggttcatc tcggaggcca gcccctgcaa
     7441 caagcgcccg aactcccgct gcgccaccgc ccgctcctcc accaccgcac ccgtccgccc
     7501 gcctgcccgg tcacccgccg gaccggtcac gaccctagcg cccgcgccag caggtcgagc
     7561 gcacccggca ggtcccgggg gagcagggcg ccgggcgggc gggtggcccg ctgccagccc
     7621 gggcccgcgg cgagcagggc gggacggacc cgggcgccgc gggtgcccca ggcggtggcg
     7681 gcgacggcgc gggcgggggc cgggtcggcg gtggcgcggg tctgcgacca gagcaggacg
     7741 gcggcggggc cggtgcgccg gacggcggcg gcgagcgcct cgacgggcag cgcggcgccg
     7801 aagatccgga acggcaggcc gcgttcggag agcgcggcgg ccaccgcctc cagcggcagg
     7861 gtgtgctgct cgccgggcat cgcggccagc agcacgggcg gtccggccgc ggccgccggg
     7921 ggttcggcga tccggcgcag cgcgctggag acgtgccagg acagcaggtg ctcgacctcg
     7981 acgtggtgtt cgccgccccc ggcggcccag ctgcgtccgg cggcgcgcag cgcgggcgtc
     8041 atcacgtccg tccaggcggc gacggcgccc agccgtccga cgctggcgtg cagcaggcgg
     8101 gccacctcgg gcgcgtccag ccgccgggcg gcccgggcca gcccgcgcac ctcgggccgc
     8161 acctgcccgg ccggggcctc gacggcggcc ggttcggcgg gccgcccggc cagcgcgggg
     8221 gcgagccggg cggcttcggc gggcggcgtg ccgcgcgcgg tgagccggca catcgcctcc
     8281 agcacggcca cgtcccgcac cgtccagcgc cggtgccgcc cctcggcccg ttctgccggg
     8341 ccgatgccgt agcggcgctc ccaggagcgg acggtggtcg gcgacacgcc gagccgccgg
     8401 gcgacctccc cggtgctcgc cgtcacttcg ggcagctctt ctggcaccgc gtcagcctac
     8461 ggcctgttcc ccggtgcccc gccgccgtcc cgggagcgcg ggcggggtag ccggaccgtc
     8521 cgccgggggc aggaccgtcc gccgggggca ggaccgtccg ccggggtccg gcctacccgc
     8581 ccaccgtcgc gcggcaggct tccgcgtagg cgcggacgag ggggtggcgg gcgtcctcgc
     8641 ggcgccaggc gagggcgtag tggctggggc cgatgccggt gacggggcgg gtggtgacgc
     8701 cggcgtgggc gatgaggggg gcgttgccgt cggcgaccag gcagacgccg aggccggcga
     8761 tcagggcctc gtaggtctcc tcgaccccgg cgacctcggc gccgatccgg ggcgggcggc
     8821 cggcccgggc gtccagggcc agccagtggt cgcgcagcgg gccggcgtcc ggggggaggg
     8881 cgaggaaggg ttcgtcgagc aggtcggcga agtcgaccgc ggggcgggcg gcgagcgggt
     8941 gggcggcggg gagggcgatc cggcggggtt cctcggcgac gaccgtccag tcgtagcggt
     9001 gcgggtcggg cagcggcagc cagacgaagg cgacgtcggt gctgccgtcg gccaggcccg
     9061 cggtggggtc gtcccaactg acctggcgca gccgggtggt gacctgcgga tgggtggcgc
     9121 cgaggcggga gcggacggcg gggagcagtc cgccgcgggc ggggctggtg ctcatgccga
     9181 cggtgagggt gctgcgctgt tcggccttgg cggcggccag ggcgtccgcg ccgcgggtcc
     9241 atccgtcgag gacctcgcgg gcgtgcggga gcagggtgtg gccggcggcg gtgagggtga
     9301 cgcggtgccg gtcgcggcgg aacagctcgg tgcccagccg ctgttcgagg gcccggacct
     9361 gtctgctgag cgcgggctgg gagacgtgca gccgttcggc ggcgcgggtg aagtgcaact
     9421 cctcggccac ggcgaggaag tagcgcaggt cgcgggtgtg gacgtccagg gccatgccga
     9481 ttggttatca ccgcgggtct tggaccggca acggggttcg gcggcaggct ggtgtccgtt
     9541 cggaagtacg gcgggcgaaa agggagttgc cgtgaacagg gtgtggctgg tgacgggtgc
     9601 gggcagcggt ttcggacggg cgctgacgct ggcggcggtg gccgcgggtg acgtggtggt
     9661 gggtgcggcg cggcgcccgg agtccttgga cgacctggtg gccgcgcacc cggaccaggt
     9721 ggaggcgctg ggcctggacg tcacggacac cgcggcggtc ggggcggcgg tggagcgggt
     9781 ggtcgcccgg cacgggcgga tcgacgtgct ggtgaacaac gcgggccgca cccacgtcgg
     9841 cgcggtcgag gagaccacgg acgaggagct gcgcgccctg ttcgacgtgc acgtcttcgg
     9901 gccggcggcg ctggtgcggg cggtgctgcc gcacatgcgg gagcggcgtg cgggcgcggt
     9961 cgtgcagatg agcagcgtgg gcgggcagct gtccttcgcg ggcttcgggg cgtacagcgc
    10021 gacgaagttc gcgctggagg ggatgtccga ggcgctcgcg gacgaggtgc gcgagtacgg
    10081 cgtcagggtg ctcgtcgtgg agccgggcgc gttccggacc tcgctgttcg cggcctcccg
    10141 ggccggggtg agcgcggacg gcggccggta cgcggcggtg gccgcgaccc gggcggccgt
    10201 ggcgggcggg gacggcgcgc agcccggcga cccggacaag gctgcggcgc tgatcctggc
    10261 cgcgctggag gcggagcggc cgccgctgcg gctggtgctg ggcgaggacg cggtggacgg
    10321 cgtgctgggg cacctggagc aggtccggga ggacgtgctg gcctgggagg agcggtccag
    10381 ggcgacggcc ttcgacgcct gatccggtgc gggcgtccgt gcctgatcag gtgcgggtgt
    10441 ccgcgtcgtc cgagagatct ctcaatgatt gagatatcct ccggccatgc cgacgcccgc
    10501 gccatccgcc ccgtccacct cccccgcgcc cgcccccggc cccgcgcccg ggcggcggct
    10561 gcgcttcctg ctgccgctcg tcctgctgct ggtctggctg gcgctcggcg gcgcgctcgg
    10621 gccgttcgcc gggcggctgg gcgaggtgtc gaccaacgac ggcgcggcgt tcctgccgcg
    10681 cagcgccgag tcgacccggg tcacccggct ggaggaggag ttccgcaccg agtcgacgct
    10741 gccggtggtg ctggtctggg agtccggctc cgggcaggtc gacgacgcgc tgcgggccga
    10801 g
//
